Addgene pDUAL_araBAD Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pDUAL_araBAD Sequencing Result


Map View

RBS NcoI (8639) KpnI (8608) Acc65I (8604) AatII (8395) ZraI (8393) MscI (8302) SphI (7823) BmgBI (7802) PfoI * (7744) CsiI - SexAI * (7648) HindIII (7083) EcoRI (6982) LacI-R (6865 .. 6884) BclI * (6534) ApaI (6344) PspOMI (6340) HpaI (6045) BglII (5814) RBS Amp-R (5555 .. 5574) ScaI (5485) PstI (4930) PaeR7I - PspXI - XhoI (4890) BsrGI (4836) EcoNI (4771) RsrII (4523) BamHI (42) pBAD Forward (59 .. 78) BsmI (190) BspEI (566) PaqCI (1262) NotI (1269) BstZ17I (1331) pRS-marker (1348 .. 1367) L4440 (1448 .. 1465) pBR322ori-F (1699 .. 1718) AlwNI (1974) SpeI (2224) T7 (2244 .. 2263) T7 promoter XbaI (2290) RBS SalI (2363) NsiI (3396) KflI - PpuMI (3792) pDUAL_araBAD 8676 bp

Sequence View

BamHI 5ʹ 3ʹ 60 araBAD promoter C C pBAD Forward a t g g a g a a a c a g t a g a g a g t t g c g a t a a a a a g c g t c a g g t a g g a t c c g c t a a t c t t a t g g t a c c t c t t t g t c a t c t c t c a a c g c t a t t t t t c g c a g t c c a t c c t a g g c g a t t a g a a t a c c

120 araBAD promoter T A T T T T T A C G A T A C C G T A pBAD Forward a t a a a a a t g c t a t g g c a t a g c a a a g t g t g a c g c c g t g c a a a t a a t c a a t g t g g a c t t t t c t a t t t t t a c g a t a c c g t a t c g t t t c a c a c t g c g g c a c g t t t a t t a g t t a c a c c t g a a a a g

180 araBAD promoter t g c c g t g a t t a t a g a c a c t t t t g t t a c g c g t t t t t g t c a t g g c t t t g g t c c c g c t t t g t t a c g g c a c t a a t a t c t g t g a a a a c a a t g c g c a a a a a c a g t a c c g a a a c c a g g g c g a a a c a a

BsmI 240 araBAD promoter a c a g a a t g c t t t t a a t a a g c g g g g t t a c c g g t t t g g t t a g c g a g a a g a g c c a g t a a a a g a t g t c t t a c g a a a a t t a t t c g c c c c a a t g g c c a a a c c a a t c g c t c t t c t c g g t c a t t t t c t

300 araBAD promoter MQYGQLVSSLNGG c g c a g t g a c g g c a a t g t c t g a t g c a a t a t g g a c a a t t g g t t t c t t c t c t g a a t g g c g g g a g c g t c a c t g c c g t t a c a g a c t a c g t t a t a c c t g t t a a c c a a a g a a g a g a c t t a c c g c c c t

360 1 5 10 15 MAEAQNDPLLPGYSFN araC SMKSMAEAQNDPLLPGYSFN g t a t g a a a a g t a t g g c t g a a g c g c a a a a t g a t c c c c t g c t g c c g g g a t a c t c g t t t a a t g c a t a c t t t t c a t a c c g a c t t c g c g t t t t a c t a g g g g a c g a c g g c c c t a t g a g c a a a t t a c

420 20 25 30 35 AHLVAGLTPIEANGYLDFFI araC AHLVAGLTPIEANGYLDFFI c c c a t c t g g t g g c g g g t t t a a c g c c g a t t g a g g c c a a c g g t t a t c t c g a t t t t t t t a t c g g g g t a g a c c a c c g c c c a a a t t g c g g c t a a c t c c g g t t g c c a a t a g a g c t a a a a a a a t a g c

480 40 45 50 55 DRPLGMKGYILNLTIRGQGV araC DRPLGMKGYILNLTIRGQGV a c c g a c c g c t g g g a a t g a a a g g t t a t a t t c t c a a t c t c a c c a t t c g c g g t c a g g g g g t g g t g g c t g g c g a c c c t t a c t t t c c a a t a t a a g a g t t a g a g t g g t a a g c g c c a g t c c c c c a c c

540 60 65 70 75 VKNQGREFVCRPGDILLFPP araC VKNQGREFVCRPGDILLFPP t g a a a a a t c a g g g a c g a g a a t t t g t t t g c c g a c c g g g t g a t a t t t t g c t g t t c c c g c c a g a c t t t t t a g t c c c t g c t c t t a a a c a a a c g g c t g g c c c a c t a t a a a a c g a c a a g g g c g g t c

BspEI 600 80 85 90 95 GEIHHYGRHPEAREWYHQWV araC GEIHHYGRHPEAREWYHQWV g a g a g a t t c a t c a c t a c g g t c g t c a t c c g g a g g c t c g c g a a t g g t a t c a c c a g t g g g t t t c t c t c t a a g t a g t g a t g c c a g c a g t a g g c c t c c g a g c g c t t a c c a t a g t g g t c a c c c a a a

660 100 105 110 115 YFRPRAYWHEWLNWPSIFAN araC YFRPRAYWHEWLNWPSIFAN a c t t t c g t c c g c g c g c c t a c t g g c a t g a a t g g c t t a a c t g g c c g t c a a t a t t t g c c a a t a t g a a a g c a g g c g c g c g g a t g a c c g t a c t t a c c g a a t t g a c c g g c a g t t a t a a a c g g t t a t

720 120 125 130 135 TGFFRPDEAHQPHFSDLFGQ araC TGFFRPDEAHQPHFSDLFGQ c g g g g t t c t t t c g c c c g g a t g a a g c g c a c c a g c c g c a t t t c a g c g a c c t g t t t g g g c a a a g c c c c a a g a a a g c g g g c c t a c t t c g c g t g g t c g g c g t a a a g t c g c t g g a c a a a c c c g t t t

780 140 145 150 155 IINAGQGEGRYSELLAINLL araC IINAGQGEGRYSELLAINLL t c a t t a a c g c c g g g c a a g g g g a a g g g c g c t a t t c g g a g c t g c t g g c g a t a a a t c t g c t t g a g t a a t t g c g g c c c g t t c c c c t t c c c g c g a t a a g c c t c g a c g a c c g c t a t t t a g a c g a a c

840 160 165 170 175 EQLLLRRMEAINESLHPPMD araC EQLLLRRMEAINESLHPPMD a g c a a t t g t t a c t g c g g c g c a t g g a a g c g a t t a a c g a g t c g c t c c a t c c a c c g a t g g a t a t c g t t a a c a a t g a c g c c g c g t a c c t t c g c t a a t t g c t c a g c g a g g t a g g t g g c t a c c t a t

900 180 185 190 195 NRVREACQYISDHLADSNFD araC NRVREACQYISDHLADSNFD a t c g g g t a c g c g a g g c t t g t c a g t a c a t c a g c g a t c a c c t g g c a g a c a g c a a t t t t g a t a t a g c c c a t g c g c t c c g a a c a g t c a t g t a g t c g c t a g t g g a c c g t c t g t c g t t a a a a c t a t

960 200 205 210 215 IASVAQHVCLSPSRLSHLFR araC IASVAQHVCLSPSRLSHLFR t c g c c a g c g t c g c a c a g c a t g t t t g c t t g t c g c c g t c g c g t c t g t c a c a t c t t t t c c g c c a g c g g t c g c a g c g t g t c g t a c a a a c g a a c a g c g g c a g c g c a g a c a g t g t a g a a a a g g c g g

1020 220 225 230 235 QQLGISVLSWREDQRISQAK araC QQLGISVLSWREDQRISQAK a g c a g t t a g g g a t t a g c g t c t t a a g c t g g c g c g a g g a c c a a c g t a t c a g c c a g g c g a a g c t c g t c a a t c c c t a a t c g c a g a a t t c g a c c g c g c t c c t g g t t g c a t a g t c g g t c c g c t t c g

1080 240 245 250 255 LLLSTTRMPIATVGRNVGFD araC LLLSTTRMPIATVGRNVGFD t g c t t t t g a g c a c c a c c c g g a t g c c t a t c g c c a c c g t c g g t c g c a a t g t t g g t t t t g a c g a c g a a a a c t c g t g g t g g g c c t a c g g a t a g c g g t g g c a g c c a g c g t t a c a a c c a a a a c t g c

1140 260 265 270 275 DQLYFSRVFKKCTGASPSEF araC DQLYFSRVFKKCTGASPSEF a t c a a c t c t a t t t c t c g c g g g t a t t t a a a a a a t g c a c c g g g g c c a g c c c g a g c g a g t t c c t a g t t g a g a t a a a g a g c g c c c a t a a a t t t t t t a c g t g g c c c c g g t c g g g c t c g c t c a a g g

1200 280 285 290 RAGCEEKVNDVAVKLS* araC rrnB T2 terminator RAGCEEKVNDVAVKLS* g t g c c g g t t g t g a a g a a a a a g t g a a t g a t g t a g c c g t c a a g t t g t c a t a a g g a g c t c a a a c a c g g c c a a c a c t t c t t t t t c a c t t a c t a c a t c g g c a g t t c a a c a g t a t t c c t c g a g t t t

1260 rrnB T2 terminator a g g c c a t c c g t c a g g a t g g c c t t c t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g c a t t c c g g t a g g c a g t c c t a c c g g a a g a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c g t a

NotIPaqCI 1320 bom c g a t t t a g c g g c c g c g t t g g c g g g t g t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t g c t a a a t c g c c g g c g c a a c c g c c c a c a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a

BstZ17I 1380 bom C G G C A T C A G A G C A G A T T G T A pRS-marker a g c g g a g t g t a t a c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c t c g c c t c a c a t a t g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g

1440 bom a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c t c t t t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g a g a a

1500 G A G C G A G T G A C T G A G C G A L4440 c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c

1560 c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t

1620 ori t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a

1680 ori t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g

1740 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a

1800 ori c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c

1860 ori t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t

1920 ori a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a

AlwNI 1980 ori a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t

2040 ori a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t

2100 ori a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a

2160 ori t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a

2220 ori t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t

SpeI 2280 T7 promoter lac operator T A A T A C G A C T C A C T A T A G G G T7 t c t a c t a g t c g a t c c c g c g a a a t t a a t a c g a c t c a c t a t a g g g g a a t t g t g a g c g g a t a a a g a t g a t c a g c t a g g g c g c t t t a a t t a t g c t g a g t g a t a t c c c c t t a a c a c t c g c c t a t t

XbaI 2340 lac operator RBS MGS c a a t t c c c c t c t a g a a a t a a t t t t g t t t a a c t t t a a g a a g g a g a t a t c a t a t g g g c a g c a g t t a a g g g g a g a t c t t t a t t a a a a c a a a t t g a a a t t c t t c c t c t a t a g t a t a c c c g t c g t

SalI 2400 1 5 HHHHHH (in frame with 6xHis) GSTRYGHFDDEAR 6xHis SHHHHHHGSTRYGHFDDEAR g c c a t c a c c a t c a c c a t c a c g g g t c g a c c c g c t a t g g t c a t t t t g a c g a t g a a g c a c g c g c g g t a g t g g t a g t g g t a g t g c c c a g c t g g g c g a t a c c a g t a a a a c t g c t a c t t c g t g c g c

2460 (in frame with 6xHis) EYVITTPHTPYPWINYLGSE EYVITTPHTPYPWINYLGSE a g t a c g t t a t c a c c a c a c c g c a t a c t c c g t a c c c t t g g a t c a a c t a t c t g g g t t c g g a a c t c a t g c a a t a g t g g t g t g g c g t a t g a g g c a t g g g a a c c t a g t t g a t a g a c c c a a g c c t t g

2520 (in frame with 6xHis) QFFSLLSHQAGGYSFYRDAK QFFSLLSHQAGGYSFYRDAK a g t t c t t c a g t c t t t t g t c g c a c c a g g c t g g c g g c t a t t c g t t t t a t c g c g a t g c c a a g a t c a a g a a g t c a g a a a a c a g c g t g g t c c g a c c g c c g a t a a g c a a a a t a g c g c t a c g g t t c t

2580 (in frame with 6xHis) MRRLTRYRYNNIPADAGGRY MRRLTRYRYNNIPADAGGRY t g c g t a g a t t g a c a a g a t a c a g a t a c a a c a a c a t a c c a g c a g a c g c g g g a g g c c g g t a t c a c g c a t c t a a c t g t t c t a t g t c t a t g t t g t t g t a t g g t c g t c t g c g c c c t c c g g c c a t a g

2640 (in frame with 6xHis) LYVNDGGDVWTPSWLPVKAD LYVNDGGDVWTPSWLPVKAD t t t a t g t a a a c g a c g g t g g g g a c g t t t g g a c g c c a t c g t g g c t t c c t g t t a a a g c a g a c t a a a t a c a t t t g c t g c c a c c c c t g c a a a c c t g c g g t a g c a c c g a a g g a c a a t t t c g t c t g a

2700 (in frame with 6xHis) LDHFEARHGLGYSTITGERN LDHFEARHGLGYSTITGERN *IMKFGSMTKAVTGNCTFSI t a g a t c a t t t t g a a g c c a g a c a t g g t c t t g g c t a c a g t a c c a t t a c a g g t g a a a g a a a t g a t c t a g t a a a a c t t c g g t c t g t a c c a g a a c c g a t g t c a t g g t a a t g t c c a c t t t c t t t a c

2760 (in frame with 6xHis) GVRVETLFFVPVGENAEVQK GVRVETLFFVPVGENAEVQK PYPNLGQKEDWDTLVRLDLF g g g t a c g g g t t g a g a c c t t g t t t t t c g t c c c a g t c g g t g a g a a c g c g g a g g t c c a g a a a g c c c a t g c c c a a c t c t g g a a c a a a a a g c a g g g t c a g c c a c t c t t g c g c c t c c a g g t c t t t c

2820 (in frame with 6xHis) VTVTNTSDSYKSLTLFSFVE VTVTNTSDSYKSLTLFSFVE NRDSIGRVRIFGQRQKRKDF t t a c g g t c a c t a a t a c c t c t g a c t c g t a t a a a t c c c t g a c g t t g t t t t c t t t t g t c g a a t a a t g c c a g t g a t t a t g g a g a c t g a g c a t a t t t a g g g a c t g c a a c a a a a g a a a a c a g c t t a

2880 (in frame with 6xHis) FCLWNAQDDQTNYQRNLSIG FCLWNAQDDQTNYQRNLSIG KTKPVRLVILSVVLTIQAYS t t t g t c t t t g g a a c g c g c a a g a c g a t c a a a c t a a c t a c c a g c g t a a t c t g a g c a t a g g a g a a a c a g a a a c c t t g c g c g t t c t g c t a g t t t g a t t g a t g g t c g c a t t a g a c t c g t a t c c t c

2940 (in frame with 6xHis) EVEVEQESPHGSAIYHRTEY EVEVEQESPHGSAIYHRTEY LNFHFLFRRMATCNIVTCLV a g g t t g a a g t g g a a c a a g a a t c t c c g c a t g g c a g t g c a a t t t a t c a c c g t a c a g a g t a c c t c c a a c t t c a c c t t g t t c t t a g a g g c g t a c c g t c a c g t t a a a t a g t g g c a t g t c t c a t g g

3000 (in frame with 6xHis) RERRDHYAVFAVNTQAEGFD RERRDHYAVFAVNTQAEGFD PLSAIVVRYEGNIRLGLTEI g g g a g a g a c g c g a t c a c t a c g c g g t a t t c g c c g t t a a t a c g c a g g c c g a g g g t t t c g a t a c c c t c t c t g c g c t a g t g a t g c g c c a t a a g c g g c a a t t a t g c g t c c g g c t c c c a a a g c t a t

3060 (in frame with 6xHis) TDRDTFVGAYNSLGEAAVPL TDRDTFVGAYNSLGEAAVPL CIAVCKHSGIVRKTFGCNGK c a g a t c g c g a c a c a t t t g t g g g a g c c t a t a a c t c g c t t g g t g a a g c c g c a g t t c c c c t t a g t c t a g c g c t g t g t a a a c a c c c t c g g a t a t t g a g c g a a c c a c t t c g g c g t c a a g g g g a a t

3120 (in frame with 6xHis) KGESANSVASGWYPIGSHSV KGESANSVASGWYPIGSHSV LSFTCIANCTSPIRNARM a g g g a g a a a g t g c a a a t a g c g t t g c a a g t g g a t g g t a t c c t a t t g g c t c t c a t a g c g t g g t c c c t c t t t c a c g t t t a t c g c a a c g t t c a c c t a c c a t a g g a t a a c c g a g a g t a t c g c a c c

3180 (in frame with 6xHis) AVSLAPGESRELVYVLGYVE AVSLAPGESRELVYVLGYVE c a g t g t c t t t a g c g c c t g g a g a a t c c a g a g a a t t g g t t t a t g t g c t t g g g t a c g t g g a a a g t c a c a g a a a t c g c g g a c c t c t t a g g t c t c t t a a c c a a a t a c a c g a a c c c a t g c a c c t t t

3240 (in frame with 6xHis) NPDEEKWADDAKQVVNKERA NPDEEKWADDAKQVVNKERA a t c c t g a t g a g g a g a a g t g g g c a g a c g a c g c a a a a c a g g t c g t g a a t a a a g a a a g a g c t c t a g g a c t a c t c c t c t t c a c c c g t c t g c t g c g t t t t g t c c a g c a c t t a t t t c t t t c t c g a g

3300 (in frame with 6xHis) HALLSRFATSEQTDAAFAAL HALLSRFATSEQTDAAFAAL a c g c t t t a t t a t c a a g a t t t g c t a c g t c c g a a c a g a c t g a c g c a g c t t t c g c c g c t t t g a t g c g a a a t a a t a g t t c t a a a c g a t g c a g g c t t g t c t g a c t g c g t c g a a a g c g g c g a a a c t

3360 (in frame with 6xHis) KDYWTDLLSTYSVSSNDEKL KDYWTDLLSTYSVSSNDEKL a g g a t t a t t g g a c g g a t t t g t t a t c g a c g t a t t c c g t t a g t t c c a a t g a c g a a a a a t t g g t c c t a a t a a c c t g c c t a a a c a a t a g c t g c a t a a g g c a a t c a a g g t t a c t g c t t t t t a a c c

NsiI 3420 (in frame with 6xHis) DRMVNIWNQYQCMVTFNMSR DRMVNIWNQYQCMVTFNMSR a c c g g a t g g t t a a t a t a t g g a a t c a g t a c c a a t g c a t g g t a a c c t t t a a c a t g a g t c g c t t g g c c t a c c a a t t a t a t a c c t t a g t c a t g g t t a c g t a c c a t t g g a a a t t g t a c t c a g c g a

3480 (in frame with 6xHis) SASFFETGIGRGMGFRDSNQ SASFFETGIGRGMGFRDSNQ c g g c g a g c t t t t t c g a g a c c g g t a t t g g g a g a g g g a t g g g g t t t c g t g a t a g c a a c c a a g g c c g c t c g a a a a a g c t c t g g c c a t a a c c c t c t c c c t a c c c c a a a g c a c t a t c g t t g g t t c

3540 (in frame with 6xHis) DLLGFVHLIPERARERIIDI DLLGFVHLIPERARERIIDI a c c t t c t g g g c t t t g t t c a c t t a a t c c c a g a g c g g g c g c g t g a g c g c a t c a t t g a c a t a g t g g a a g a c c c g a a a c a a g t g a a t t a g g g t c t c g c c c g c g c a c t c g c g t a g t a a c t g t a t c

3600 (in frame with 6xHis) ASTQFADGSAYHQYQPLTKR ASTQFADGSAYHQYQPLTKR c g t c a a c c c a g t t c g c g g a c g g t t c c g c t t a t c a c c a a t a c c a a c c t t t a a c g a a g c g c g g c a g t t g g g t c a a g c g c c t g c c a a g g c g a a t a g t g g t t a t g g t t g g a a a t t g c t t c g c g c

3660 (in frame with 6xHis) GNNDIGSGFNDDPLWLIAGT GNNDIGSGFNDDPLWLIAGT g t a a c a a c g a c a t t g g c t c t g g g t t t a a c g a c g a t c c t c t t t g g t t a a t c g c g g g a a c t g c a t t g t t g c t g t a a c c g a g a c c c a a a t t g c t g c t a g g a g a a a c c a a t t a g c g c c c t t g a c

3720 (in frame with 6xHis) AAYIKETGDFSILDEPVPFD AAYIKETGDFSILDEPVPFD c c g c c t a c a t c a a a g a a a c t g g t g a c t t c t c c a t a t t a g a t g a g c c c g t a c c t t t t g a t a g g c g g a t g t a g t t t c t t t g a c c a c t g a a g a g g t a t a a t c t a c t c g g g c a t g g a a a a c t a t

3780 (in frame with 6xHis) NEPGSEVPLFEHLTRSFEFT NEPGSEVPLFEHLTRSFEFT a c g a a c c g g g g t c a g a a g t t c c g t t a t t t g a g c a c c t g a c c c g t a g t t t t g a g t t t a c c g t g c t t g g c c c c a g t c t t c a a g g c a a t a a a c t c g t g g a c t g g g c a t c a a a a c t c a a a t g g c

PpuMIKflI 3840 (in frame with 6xHis) VTHRGPHGLPLIGRADWNDC VTHRGPHGLPLIGRADWNDC t a a c c c a t c g g g g t c c c c a c g g t t t g c c t c t t a t t g g c c g c g c a g a t t g g a a c g a t t g t t a t t g g g t a g c c c c a g g g g t g c c a a a c g g a g a a t a a c c g g c g c g t c t a a c c t t g c t a a c a a

3900 (in frame with 6xHis) LNLNCFSTTPGESFQTTENQ LNLNCFSTTPGESFQTTENQ t g a a t c t t a a t t g c t t c a g t a c a a c c c c g g g c g a g t c a t t t c a g a c a a c g g a g a a c c a a g a c t t a g a a t t a a c g a a g t c a t g t t g g g g c c c g c t c a g t a a a g t c t g t t g c c t c t t g g t t c

3960 (in frame with 6xHis) AGGVAESTFIAAQFVLYGEQ AGGVAESTFIAAQFVLYGEQ c c g g t g g c g t a g c g g a g t c a a c g t t c a t a g c c g c g c a a t t c g t t t t a t a t g g g g a g c a g t g g c c a c c g c a t c g c c t c a g t t g c a a g t a t c g g c g c g t t a a g c a a a a t a t a c c c c t c g t c a

4020 (in frame with 6xHis) YAELAARRGLADVADRARGH YAELAARRGLADVADRARGH a t g c c g a g t t g g c a g c g c g g c g c g g g t t g g c c g a c g t a g c c g a t a g a g c a a g a g g g c a t g t a c g g c t c a a c c g t c g c g c c g c g c c c a a c c g g c t g c a t c g g c t a t c t c g t t c t c c c g t a c

4080 (in frame with 6xHis) VAEMRDALLTDGWDGSWFLR VAEMRDALLTDGWDGSWFLR t g g c t g a a a t g a g a g a c g c a c t t c t g a c c g a c g g g t g g g a c g g c a g c t g g t t c t t g a g a g a c c g a c t t t a c t c t c t g c g t g a a g a c t g g c t g c c c a c c c t g c c g t c g a c c a a g a a c t c t c

4140 (in frame with 6xHis) AYDYYGNPIGTDAHDEGKIW AYDYYGNPIGTDAHDEGKIW c t t a t g a c t a t t a t g g a a a c c c t a t c g g c a c c g a c g c a c a t g a c g a a g g g a a g a t t t g g a g a a t a c t g a t a a t a c c t t t g g g a t a g c c g t g g c t g c g t g t a c t g c t t c c c t t c t a a a c c t

4200 (in frame with 6xHis) IEPQGFAVMAGVGVGEGPQD IEPQGFAVMAGVGVGEGPQD t c g a a c c g c a a g g g t t c g c g g t g a t g g c t g g a g t c g g t g t a g g c g a a g g g c c t c a a g a t a a g c t t g g c g t t c c c a a g c g c c a c t a c c g a c c t c a g c c a c a t c c g c t t c c c g g a g t t c t a t

4260 (in frame with 6xHis) TDAPAIKALDSVNEMLATDH TDAPAIKALDSVNEMLATDH c g g a c g c t c c c g c t a t t a a g g c g c t t g a t t c t g t g a a c g a a a t g t t g g c a a c c g a t c a c g g c c t g c g a g g g c g a t a a t t c c g c g a a c t a a g a c a c t t g c t t t a c a a c c g t t g g c t a g t g c

4320 (in frame with 6xHis) GMVLQYPAYTTYQVHMGEVS GMVLQYPAYTTYQVHMGEVS g t a t g g t c c t t c a a t a c c c a g c a t a c a c t a c a t a c c a a g t a c a c a t g g g t g a a g t a t c c a c a t a c c a g g a a g t t a t g g g t c g t a t g t g a t g t a t g g t t c a t g t g t a c c c a c t t c a t a g g t

4380 (in frame with 6xHis) TYPPGYKENGGIFCHNNPWV TYPPGYKENGGIFCHNNPWV c t t a t c c a c c c g g g t a t a a a g a g a a t g g a g g t a t a t t t t g c c a c a a t a a t c c t t g g g t t a g a a t a g g t g g g c c c a t a t t t c t c t t a c c t c c a t a t a a a a c g g t g t t a t t a g g a a c c c a a t

4440 (in frame with 6xHis) IIAETVVGRGGRAFDYYKRI IIAETVVGRGGRAFDYYKRI t c a t a g c g g a a a c t g t c g t c g g a a g a g g c g g a c g g g c a t t t g a t t a c t a c a a g a g a a t t a a g t a t c g c c t t t g a c a g c a g c c t t c t c c g c c t g c c c g t a a a c t a a t g a t g t t c t c t t a a t

4500 (in frame with 6xHis) TPAYREDISDVHRLEPYVYA TPAYREDISDVHRLEPYVYA c t c c c g c g t a t c g c g a g g a c a t a a g c g a c g t t c a c c g t c t t g a a c c c t a t g t c t a t g c a c g a g g g c g c a t a g c g c t c c t g t a t t c g c t g c a a g t g g c a g a a c t t g g g a t a c a g a t a c g t g

RsrII 4560 (in frame with 6xHis) QMIAGKEAVRHGEAKNSWLT QMIAGKEAVRHGEAKNSWLT a a a t g a t a g c a g g g a a g g a g g c g g t c c g t c a c g g t g a a g c a a a g a a c a g c t g g c t t a c c g t t t a c t a t c g t c c c t t c c t c c g c c a g g c a g t g c c a c t t c g t t t c t t g t c g a c c g a a t g g c

4620 (in frame with 6xHis) GTAAWNFVTVSQYLLGVRPE GTAAWNFVTVSQYLLGVRPE g g a c t g c g g c t t g g a a c t t t g t g a c c g t g t c a c a g t a c c t t c t t g g g g t t c g c c c a g a g t c c t g a c g c c g a a c c t t g a a a c a c t g g c a c a g t g t c a t g g a a g a a c c c c a a g c g g g t c t c a

4680 (in frame with 6xHis) YDGLVVDPQIGPDVPSFTVT YDGLVVDPQIGPDVPSFTVT a c g a c g g c t t g g t g g t a g a c c c g c a g a t c g g a c c t g a c g t a c c t a g c t t t a c g g t t a c g c t g c t g c c g a a c c a c c a t c t g g g c g t c t a g c c t g g a c t g c a t g g a t c g a a a t g c c a a t g c g

4740 (in frame with 6xHis) RVARGATYEITVTNSGTDGS RVARGATYEITVTNSGTDGS g t g t c g c c c g t g g a g c c a c t t a c g a g a t a a c a g t c a c t a a c a g t g g g a c c g a c g g a t c a c c a c a g c g g g c a c c t c g g t g a a t g c t c t a t t g t c a g t g a t t g t c a c c c t g g c t g c c t a g t g

EcoNI 4800 (in frame with 6xHis) RGRLVVDGTPVEGNLVPYAP RGRLVVDGTPVEGNLVPYAP g c g g t c g c c t t g t c g t t g a t g g a a c a c c t g t a g a a g g c a a c t t g g t t c c t t a t g c c c c c g c g c c a g c g g a a c a g c a a c t a c c t t g t g g a c a t c t t c c g t t g a a c c a a g g a a t a c g g g g g c

BsrGI 4860 (in frame with 6xHis) AGSTVRVDVTL* T7 terminator AGSTVRVDVTL* c a g g g t c a a c a g t t c g t g t c g a t g t g a c a t t g t a g t g t a c a c t a g c a t a a c c c c t t g g g g g t c c c a g t t g t c a a g c a c a g c t a c a c t g t a a c a t c a c a t g t g a t c g t a t t g g g g a a c c c c

PaeR7IPspXIXhoI 4920 T7 terminator c c t c t a a a c g g g t c t t g a g g g g t t t t t t g c t c g a g t a a a a a a a a t c c t t a g c t t t c g c t a g g a g a t t t g c c c a g a a c t c c c c a a a a a a c g a g c t c a t t t t t t t t a g g a a t c g a a a g c g a t

PstI 4980 285 280 275 *WHKILSAGIEAIQRN AmpR *WHKILSAGIEAIQRN a g g a t c t g c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t t c c t a g a c g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a

5040 270 265 260 255 REDMTAQSGTTYIVVIRSPK AmpR REDMTAQSGTTYIVVIRSPK c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t

5100 250 245 240 235 GDPGLAAIIGRSGREGAGSK AmpR GDPGLAAIIGRSGREGAGSK c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t

5160 230 225 220 215 DAIFWGAPLASRLLPGAVKD AmpR DAIFWGAPLASRLLPGAVKD t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g

5220 210 205 200 195 AEMWDILQQRSALTLLEGTL AmpR AEMWDILQQRSALTLLEGTL g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a

5280 190 185 180 175 LKRLTTAMAVPMTTDREDNP AmpR LKRLTTAMAVPMTTDREDNP a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a

5340 170 165 160 155 IAENLEPEWRDLRTVHDGMN AmpR IAENLEPEWRDLRTVHDGMN a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c

5400 150 145 140 135 HLFATLEKPGGITTLLLNAA AmpR HLFATLEKPGGITTLLLNAA t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t

5460 130 125 120 115 TNDSMTIAASCLERVTMGDT AmpR TNDSMTIAASCLERVTMGDT g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t

ScaI 5520 110 105 100 95 LHKETVPSYEVLDNQSYHIR AmpR LHKETVPSYEVLDNQSYHIR a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c

5580 90 85 80 75 RGLQEQGADIRSLVAGCLLV AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RGLQEQGADIRSLVAGCLLV c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a

5640 70 65 60 55 KFTSMMPFREEPRFSELIKG AmpR KFTSMMPFREEPRFSELIKG t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c

5700 50 45 40 35 SNLDLEIYGVRAGLQDEADK AmpR SNLDLEIYGVRAGLQDEADK c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a

5760 30 25 VKVLTEPH 20 15 signal sequence AFVPLCFAAFFP AmpR VKVLTEPHAFVPLCFAAFFP a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t

BglII 5820 10 5 1 signal sequence ILAVRFHQISM AmpR RBS ILAVRFHQISM a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t g c t t a a t t t c t c c t c t t t a a t a g a t c t t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a c g a a t t a a a g a g g a g a a a t t a t c t a g a a

5880 *QGSELRSVQRALQMLSDAL c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g

5940 ARPSATQTNPALTTKRKVLS g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c c a g g g t g g t t t t t c t t t t c a c c a g t g a g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g g t c c c a c c a a a a a g a a a a g t g g t c a c t c

6000 VPLLQNGKVAQGQSLQLLRD a c g g g c a a c a g c t g a t t g c c c t t c a c c g c c t g g c c c t g a g a g a g t t g c a g c a a g c g g t c c t g c c c g t t g t c g a c t a a c g g g a a g t g g c g g a c c g g g a c t c t c t c a a c g t c g t t c g c c a g g

HpaI 6060 VSTQGLLRFDQKITTLPPIY a c g c t g g t t t g c c c c a g c a g g c g a a a a t c c t g t t t g a t g g t g g t t a a c g g c g g g a t a t a a t g c g a c c a a a c g g g g t c g t c c g c t t t t a g g a c a a a c t a c c a c c a a t t g c c g c c c t a t a t t

6120 CSSDETDDYGVVSIDAGVRL c a t g a g c t g t c c t c g g t a t c g t c g t a t c c c a c t a c c g a g a t a t c c g c a c c a a c g c g c a g c g t a c t c g a c a g g a g c c a t a g c a g c a t a g g g t g a t g g c t c t a t a g g c g t g g t t g c g c g t c g

6180 GSETIARMAGLAMQDNAVLM c c g g a c t c g g t a a t g g c g c g c a t t g c g c c c a g c g c c a t c t g a t c g t t g g c a a c c a g c a t c g g c c t g a g c c a t t a c c g c g c g t a a c g c g g g t c g c g g t a g a c t a g c a a c c g t t g g t c g t a g

6240 ATPVIGENLMQMTQQFGSMA g c a g t g g g a a c g a t g c c c t c a t t c a g c a t t t g c a t g g t t t g t t g a a a a c c g g a c a t g g c a c g t c a c c c t t g c t a c g g g a g t a a g t c g t a a a c g t a c c a a a c a a c t t t t g g c c t g t a c c g t

6300 SWDGEREAIPQIQNRTLYKH c t c c a g t c g c c t t c c c g t t c c g c t a t c g g c t g a a t t t g a t t g c g a g t g a g a t a t t t a t g c g a g g t c a g c g g a a g g g c a a g g c g a t a g c c g a c t t a a a c t a a c g c t c a c t c t a t a a a t a c g

PspOMIApaI 6360 WGALRLRASVSSLPGALLAI c a g c c a g c c a g a c g c a g a c g c g c c g a g a c a g a a c t t a a t g g g c c c g c t a a c a g c g c g a t t g t c g g t c g g t c t g c g t c t g c g c g g c t c t g t c t t g a a t t a c c c g g g c g a t t g t c g c g c t a a

6420 QQHGLAVLHEVGLRTGDEHS t g c t g g t g a c c c a a t g c g a c c a g a t g c t c c a c g c c c a g t c g c g t a c c g t c c t c a t g g g a g a c g a c c a c t g g g t t a c g c t g g t c t a c g a g g t g c g g g t c a g c g c a t g g c a g g a g t a c c c t c

6480 FIISNIPHRTLSMLFYRRFM a a a a t a a t a c t g t t g a t g g g a t g t c t g g t c a g a g a c a t c a a g a a a t a a c g c c g g a a c a t t t t t t a t t a t g a c a a c t a c c c t a c a g a c c a g t c t c t g t a g t t c t t t a t t g c g g c c t t g t a a

BclI* 6540 LAPLKWLLPMRTM a g t g c a g g c a g c t t c c a c a g c a a t g g c a t c c t g g t c a t c c a g c g g a t a g t t a a t g a t c a g t c a c g t c c g t c g a a g g t g t c g t t a c c g t a g g a c c a g t a g g t c g c c t a t c a a t t a c t a g t c

6600 c c c a c t g a c g c g t t g c g c g a g a a g a t t g t g c a c c g c c g c t t t a c a g g c t t c g a c g c c g c t g g g t g a c t g c g c a a c g c g c t c t t c t a a c a c g t g g c g g c g a a a t g t c c g a a g c t g c g g c g a

6660 t c g t t c t a c c a t c g a c a c c a c c a c g c t g g c a c c c a g t t g a t c g g c g c g a g a t t t a a t c g c a g c a a g a t g g t a g c t g t g g t g g t g c g a c c g t g g g t c a a c t a g c c g c g c t c t a a a t t a g c g

6720 c g c g a c a a t t t g c g a c g g c g c g t g c a g g g c c a g a c t g g a g g t g g c a a c g c c a a t c a g c a a g c g c t g t t a a a c g c t g c c g c g c a c g t c c c g g t c t g a c c t c c a c c g t t g c g g t t a g t c g t t

6780 c g a c t g t t t g c c c g c c a g t t g t t g t g c c a c g c g g t t g g g a a t g t a a t t c a g c t c c g c c a t g c t g a c a a a c g g g c g g t c a a c a a c a c g g t g c g c c a a c c c t t a c a t t a a g t c g a g g c g g t a

6840 c g c c g c t t c c a c t t t t t c c c g c g t t t t c g c a g a a a c g t g g c t g g c c t g g t t c a c c a c g c g g c g g c g a a g g t g a a a a a g g g c g c a a a a g c g t c t t t g c a c c g a c c g g a c c a a g t g g t g c g c

6900 G G C A T A C T C T G C G A C A T C G T LacI-R g g a a a c g g t c t g a t a a g a g a c a c c g g c a t a c t c t g c g a c a t c g t a t a a c g t t a c t g g t t t c c t t t g c c a g a c t a t t c t c t g t g g c c g t a t g a g a c g c t g t a g c a t a t t g c a a t g a c c a a a

6960 lacIq promoter c a c a t t c a c c a c c c t g a a t t g a c t c t c t t c c g g g c g c t a t c a t g c c a t a c c g c g a a a g g t g t g t a a g t g g t g g g a c t t a a c t g a g a g a a g g c c c g c g a t a g t a c g g t a t g g c g c t t t c c a

EcoRI 7020 lacIq promoter lambda t0 terminator t t t g c a c c a t t c g a t g g t g t c g a a t t c a t t c t c a c c a a t a a a a a a c g c c c g g c g g c a a c c a a a c g t g g t a a g c t a c c a c a g c t t a a g t a a g a g t g g t t a t t t t t t g c g g g c c g c c g t t g g

7080 lambda t0 terminator g a g c g t t c t g a a c a a a t c c a g a t g g a g t t c t g a g g t c a t t a c t g g a t c t a t c a a c a g g a g c t c g c a a g a c t t g t t t a g g t c t a c c t c a a g a c t c c a g t a a t g a c c t a g a t a g t t g t c c t c

HindIII 7140 (in frame with Strep-Tag II) *AVVPPNAILDDSRHDG lambda t0 terminator *AVVPPNAILDDSRHDG t c a a g c t t t t a c g c a a c a a c c g g c g g a t t a g c g a t c a g g t c g t c g g a g c g a t g g t c a c c a a g t t c g a a a a t g c g t t g t t g g c c g c c t a a t c g c t a g t c c a g c a g c c t c g c t a c c a g t g g t

7200 (in frame with Strep-Tag II) ASDEWELMAVPTTNDVGLGR ASDEWELMAVPTTNDVGLGR g c a c t g t c t t c c c a t t c c a g c a t t g c g a c c g g c g t g g t a t t a t c a a c a c c c a g g c c g c g t c g t g a c a g a a g g g t a a g g t c g t a a c g c t g g c c g c a c c a t a a t a g t t g t g g g t c c g g c g c a

7260 (in frame with Strep-Tag II) KPEFTLTAQSTEGRWTFSIS KPEFTLTAQSTEGRWTFSIS t t c g g t t c g a a c g t c a g g g t g g c c t g c g a c g t t t c a c c a c g c c a g g t a a a g g a a a t a c t c a a g c c a a g c t t g c a g t c c c a c c g g a c g c t g c a a a g t g g t g c g g t c c a t t t c c t t t a t g a g

7320 (in frame with Strep-Tag II) TDDDTTYSFTGDFADLENRF TDDDTTYSFTGDFADLENRF g t a t c g t c a t c c g t g g t a t a t g a g a a g g t g c c g t c a a a a g c a t c c a g t t c a t t g c g a a a t c a t a g c a g t a g g c a c c a t a t a c t c t t c c a c g g c a g t t t t c g t a g g t c a a g t a a c g c t t t a

7380 (in frame with Strep-Tag II) KALANLAKVVPRKLNEDIEA KALANLAKVVPRKLNEDIEA t t c g c c a g a g c g t t c a g t g c t t t a a c c a c c g g a c g t t t c a g a t t t t c a t c g a t t t c g g c c a a g c g g t c t c g c a a g t c a c g a a a t t g g t g g c c t g c a a a g t c t a a a a g t a g c t a a a g c c g g

7440 (in frame with Strep-Tag II) TSYYHRNIDRGNNTKRLLEM TSYYHRNIDRGNNTKRLLEM g t g c t g t a a t a a t g g c g g t t a a t g t c a c g a c c a t t g t t g g t t t t g c g c a g c a g t t c c a t a c a c g a c a t t a t t a c c g c c a a t t a c a g t g c t g g t a a c a a c c a a a a c g c g t c g t c a a g g t a t

7500 (in frame with Strep-Tag II) DNKGALAGVYYVQPVGPLFF DNKGALAGVYYVQPVGPLFF t c a t t t t t a c c t g c c a g g g c g c c c a c g t a a t a g a c t t g c g g a a c a c c c g g c a g g a a a a a c a g t a a a a a t g g a c g g t c c c g c g g g t g c a t t a t c t g a a c g c c t t g t g g g c c g t c c t t t t t g

7560 (in frame with Strep-Tag II) QVARAAIYHQDNCGLASYYT QVARAAIYHQDNCGLASYYT t g c a c c g c a c g c g c a g c g a t g t a g t g c t g a t c g t t a c a g c c c a g a g c g g a g t a g t a g g t a a c g t g g c g t g c g c g t c g c t a c a t c a c g a c t a g c a a t g t c g g g t c t c g c c t c a t c a t c c a t

7620 (in frame with Strep-Tag II) SNVQYLDLNSAAAGTAAQSE SNVQYLDLNSAAAGTAAQSE c t a t t a a c t t g g t a c a g a t c c a g g t t c g a t g c c g c c g c a c c g g t a g c t g c c t g t g a t t c g g a t a a t t g a a c c a t g t c t a g g t c c a a g c t a c g g c g g c g t g g c c a t c g a c g g a c a c t a a g c

SexAI*CsiI 7680 (in frame with Strep-Tag II) GHTNAHITNVLNDVDEDPVL GHTNAHITNVLNDVDEDPVL c c g t g g g t g t t c g c a t g a a t c g t a t t c a c c a g g t t a t c c a c a t c t t c g t c c g g c a c c a g a g g c a c c c a c a a g c g t a c t t a g c a t a a g t g g t c c a a t a g g t g t a g a a g c a g g c c g t g g t c t

7740 (in frame with Strep-Tag II) GKLSRDLQDSGIDIVGIGDH M GKLSRDLQDSGIDIVGIGDH c c t t t c a g c g a g c g g t c c a g c t g a t c t g a a c c g a t a t c a a t a a c g c c g a t g c c g t c a t g c g g a a a g t c g c t c g c c a g g t c g a c t a g a c t t g g c t a t a g t t a t t g c g g c t a c g g c a g t a c g

PfoI* 7800 (in frame with Strep-Tag II) TDLVTVANNPRIDTWHAVPE RIQDGNSIVRTNIRPMGHRF TDLVTVANNPRIDTWHAVPE g t a t c c a g g a c g g t a a c a g c a t t g t t c g g a c g a a t a t c c g t c c a a t g g g c c a c c g g t t c g c a t a g g t c c t g c c a t t g t c g t a a c a a g c c t g c t t a t a g g c a g g t t a c c c g g t g g c c a a g c

SphIBmgBI 7860 (in frame with Strep-Tag II) VHGTSLAHLLLPPLAFDYVR DVAGTQGMQQQRRQGKVINA VHGTSLAHLLLPPLAFDYVR a c g t g g c c g g t a c t c a g g g c a t g c a g c a g c a g c g g c g g c a g g g c a a a g t c a t a a a c g c g a t g c a c c g g c c a t g a g t c c c g t a c g t c g t c g t c g c c g c c g t c c c g t t t c a g t a t t t g c g c t

7920 (in frame with Strep-Tag II) DVKSAIEVQKKYYSHVEILI IHFRRDFHLFFVIAVDFDQN DVKSAIEVQKKYYSHVEILI t c c a c t t t a g a c g c g a t t t c c a c c t g t t t t t t g t a a t a g c t g t g g a c t t c g a t c a g a a t t a g g t g a a a t c t g c g c t a a a g g t g g a c a a a a a a c a t t a t c g a c a c c t g a a g c t a g t c t t a a

7980 (in frame with Strep-Tag II) ELGRKVGEERLRSILKFTKP FQTAFHAFFAQTGNQFERFR ELGRKVGEERLRSILKFTKP t c c a g a c c g c g t t t c a c g c c t t c t t c g c g c a g a c g g g a a a t c a g t t t g a a c g t t t t c g g g a g g t c t g g c g c a a a g t g c g g a a g a a g c g c g t c t g c c c t t t a g t c a a a c t t g c a a a a g c c c

8040 (in frame with Strep-Tag II) TMFCSTGAEKAGYGVADLRI GHKATGACFFGTVANRIQAD TMFCSTGAEKAGYGVADLRI g t c a t a a a g c a a c t g g t g c c t g c t t c t t t g g c a c c g t a g c c a a c c g c a t c c a g g c g g a t a c a g t a t t t c g t t g a c c a c g g a c g a a g a a a c c g t g g c a t c g g t t g g c g t a g g t c c g c c t a t

8100 (in frame with Strep-Tag II) YSVHSAAMQDFISMLYEWGK IRHMAGRHLVENGHQIFPTF YSVHSAAMQDFISMLYEWGK t a a g a c a c a t g g c t g g c c g c c a t t t g g t c g a a a a t g g a c a t c a g a t a t t c c c a a c c t t t a a t t c t g t g t a c c g a c c g g c g g t a a a c c a g c t t t t a c c t g t a g t c t a t a a g g g t t g g a a a t

8160 (in frame with Strep-Tag II) DSDTDIDVQQPTFSVWVLRT ITVRIDVHLLRGKRHPDQTR DSDTDIDVQQPTFSVWVLRT t c a c t g t c c g t a t c g a t g t c c a c t t g c t g c g g g g t a a a a g a c a c c c a g a c c a g a c g c g t t a g t g a c a g g c a t a g c t a c a g g t g a a c g a c g c c c c a t t t t c t g t g g g t c t g g t c t g c g c a a

8220 (in frame with Strep-Tag II) KGAFKYHTFPLGPRPRYIGA FACEFVVGKRQTRTRTINAC KGAFKYHTFPLGPRPRYIGA t t g c c t g c g a a t t t g t a g t g g g t a a a c g g c a g a c c c g g a c g c g g a c g a t a a a t g c c t g c c a a c g g a c g c t t a a a c a t c a c c c a t t t g c c g t c t g g g c c t g c g c c t g c t a t t t a c g g a c g g

8280 (in frame with Strep-Tag II) LDEETAGNPFVSSMTLFMPY QVFFRGTVREHAAHGQKHRV LDEETAGNPFVSSMTLFMPY a g g t c t t c t t c c g t g g c a c c g t t c g g g a a c a c g c t g c t c a t g g t c a g a a a c a t c g g g t a a t c c a g a a g a a g g c a c c g t g g c a a g c c c t t g t g c g a c g a g t a c c a g t c t t t g t a g c c c a t t

MscI 8340 (in frame with Strep-Tag II) YESEEGKALVDQFQKSEWSM IF* YESEEGKALVDQFQKSEWSM t a t t c t g a t t c t t c g c c t t t g g c c a g a a c a t c t t g g a a c t g t t t c g a t t c c c a t g a c a t g a t a a g a c t a a g a a g c g g a a a c c g g t c t t g t a g a a c c t t g a c a a a g c t a a g g g t a c t g t a c

ZraIAatII 8400 (in frame with Strep-Tag II) HNVIADVMINHTKSLEAVDD HNVIADVMINHTKSLEAVDD t g a t t a a c g a t t g c a t c c a c c a t a a t g t t g t g g g t t t t a g a c a g t t c g g c g a c g t c a t c c a c t a a t t g c t a a c g t a g g t g g t a t t a c a a c a c c c a a a a t c t g t c a a g c c g c t g c a g t a g g

8460 (in frame with Strep-Tag II) WSGLREDVKTHDIPDFGADA WSGLREDVKTHDIPDFGADA c a g c t a c c c a g a c g t t c a t c a a c t t t g g t a t g g t c g a t c g g a t c g a a a c c t g c g t c g g c g g t c g a t g g g t c t g c a a g t a g t t g a a a c c a t a c c a g c t a g c c t a g c t t t g g a c g c a g c c g c

8520 (in frame with Strep-Tag II) GDFPTFFPLIHVGDYVGDFR GDFPTFFPLIHVGDYVGDFR c c a t c a a a c g g g g t g a a a a a c g g c a g a a t g t g g a c a c c g t c a t a a a c g c c a t c a a a g c g g g g t a g t t t g c c c c a c t t t t t g c c g t c t t a c a c c t g t g g c a g t a t t t g c g g t a g t t t c g c c

8580 (in frame with Strep-Tag II) TRLIDTMSKITGDGLRDAYT TRLIDTMSKITGDGLRDAYT g t a c g c a g g a t g t c c g t c a t g c t t t t a a t g g t g c c a t c a c c c a g a c g a t c c g c a t a g g t a c a t g c g t c c t a c a g g c a g t a c g a a a a t t a c c a c g g t a g t g g g t c t g c t a g g c g t a t c c a t

NcoIAcc65IKpnI 8640 (in frame with Strep-Tag II) ILQVKNKMTG 5 1 KEFQPHSW Strep-Tag II ILQVKNKMTGKEFQPHSWSA a t c a g t t g g a c t t t a t t t t t c a t g g t a c c c t t t t c g a a c t g c g g g t g g c t c c a g c t t g c c t a g t c a a c c t g a a a t a a a a a g t a c c a t g g g a a a a g c t t g a c g c c c a c c g a g g t c g a a c g g

3ʹ 5ʹ 8676 RBS M a t g g a g t c t t t c t c c t c t t t a t c c a a a a a a a c g g g t t a c c t c a g a a a g a g g a g a a a t a g g t t t t t t t g c c c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.