Addgene TagBFP_A1aY1 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: TagBFP_A1aY1 Sequencing Result


Map View

MluI (11,789) EcoRI (11,600) KflI - PpuMI (11,506) AleI (11,149) BbvCI (10,996) NotI (10,720) NruI * (10,409) PmlI (9866) EBV-rev (9366 .. 9385) SV40pA-R (9312 .. 9331) HpaI (9274) SV40 NLS AgeI (7923) AvrII (7433) SfiI (7386) pBABE 3' (7114 .. 7134) L4440 (7027 .. 7044) pBR322ori-F (6774 .. 6793) AhdI (6041) NdeI (250) SnaBI (356) AscI (503) chicken β-actin promoter BlpI (1154) BsiWI (1186) MreI (1312) pCAGGS-5 (1598 .. 1620) XbaI (1627) pCAG-F (1682 .. 1701) NheI (1733) BmtI (1737) BsrGI (1782) PasI (1963) MscI (2399) BamHI (2657) SalI (2669) Acc65I (2793) KpnI (2797) PacI (3415) PflFI - Tth111I (3579) pBRrevBam (4651 .. 4670) pRS-marker (4884 .. 4903) pGEX 3' (5003 .. 5025) pBRforEco (5063 .. 5081) Amp-R (5472 .. 5491) PvuI (5672) FspI (5818) TagBFP_A1aY1 11,795 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g

120 CMV enhancer c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t

180 CMV enhancer a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t

240 CMV enhancer c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g

NdeI 300 CMV enhancer a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a

SnaBI 360 CMV enhancer g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a

420 CMV enhancer chicken β- actin promoter t a g t c a t c g c t a t t a c c a t g g t c g a g g t g a g c c c c a c g t t c t g c t t c a c t c t c c c c a t c t a t c a g t a g c g a t a a t g g t a c c a g c t c c a c t c g g g g t g c a a g a c g a a g t g a g a g g g g t a g a

480 chicken β- actin promoter c c c c c c c c t c c c c a c c c c c a a t t t t g t a t t t a t t t a t t t t t t a a t t a t t t t g t g c a g c g a g g g g g g g g a g g g g t g g g g g t t a a a a c a t a a a t a a a t a a a a a a t t a a t a a a a c a c g t c g c t

AscI 540 chicken β- actin promoter t g g g g g c g g g g g g g g g g g g g g g g c g c g c c r g g g g g g g g g g g g g g g g g s g r g g g g s g g g g s a c c c c c g c c c c c c c c c c c c c c c c g c g c g g y c c c c c c c c c c c c c c c c c s c y c c c c s c c c c s

600 chicken β- actin promoter g g g g c g a g g c g g a g a g g t g c g g c g g c a g c c a a t c a g a g c g g c g c g c t c c g a a a g t t t c c t c c c c g c t c c g c c t c t c c a c g c c g c c g t c g g t t a g t c t c g c c g c g c g a g g c t t t c a a a g g a

660 chicken β- actin promoter chimeric intron t t t a t g g c g a g g c g g c g g c g g c g g c g g c c c t a t a a a a a g c g a a g c g c g c g g c g g g c g g g a a a a t a c c g c t c c g c c g c c g c c g c c g c c g g g a t a t t t t t c g c t t c g c g c g c c g c c c g c c c t

720 chimeric intron g t c g c t g c g c g c t g c c t t c g c c c c g t g c c c c g c t c c g c c g c c g c c t c g c g c c g c c c g c c c c a g c g a c g c g c g a c g g a a g c g g g g c a c g g g g c g a g g c g g c g g c g g a g c g c g g c g g g c g g g

780 chimeric intron *EWLHAPPVARRRR c g g c t c t g a c t g a c c g c g t t a c t c c c a c a g g t g a g c g g g c g g g a c g g c c c t t c t c c t c c g g c c g a g a c t g a c t g g c g c a a t g a g g g t g t c c a c t c g c c c g c c c t g c c g g g a a g a g g a g g c

840 chimeric intron ATILAQNLSPKNRKQPQTFA g g c t g t a a t t a g c g c t t g g t t t a a t g a c g g c t t g t t t c t t t t c t g t g g c t g c g t g a a a g c c c g a c a t t a a t c g c g a a c c a a a t t a c t g c c g a a c a a a g a a a a g a c a c c g a c g c a c t t t c g

900 chimeric intron KLPEPLARQAPPAARPTRAH c t t g a g g g g c t c c g g g a g g g c c c t t t g t g c g g g g g g a g c g g c t c g g g g g g t g c g t g c g t g g a a c t c c c c g a g g c c c t c c c g g g a a a c a c g c c c c c c t c g c c g a g c c c c c c a c g c a c g c a c

960 chimeric intron THAHPAGRAAGRQGAATLAA t g t g t g t g c g t g g g g a g c g c c g c g t g c g g c t c c g c g c t g c c c g g c g g c t g t g a g c g c t g c a c a c a c a c g c a c c c c t c g c g g c g c a c g c c g a g g c g c g a c g g g c c g c c g a c a c t c g c g a c g

1020 chimeric intron PAARPKTREATHALPARGPA g g g c g c g g c g c g g g g c t t t g t g c g c t c c g c a g t g t g c g c g a g g g g a g c g c g g c c g g g g g c c c c g c g c c g c g c c c c g a a a c a c g c g a g g c g t c a c a c g c g c t c c c c t c g c g c c g g c c c c c g

1080 chimeric intron TGRPAPPAALPVFAAHPTHA g g t g c c c c g c g g t g c g g g g g g g g c t g c g a g g g g a a c a a a g g c t g c g t g c g g g g t g t g t g c c c a c g g g g c g c c a c g c c c c c c c c g a c g c t c c c c t t g t t t c c g a c g c a c g c c c c a c a c a c g

1140 chimeric intron HPPSCPTHARRDPQLGGQVG g t g g g g g g g t g a g c a g g g g g t g t g g g c g c g t c g g t c g g g c t g c a a c c c c c c c t g c a c c c c c a c c c c c c c a c t c g t c c c c c a c a c c c g c g c a g c c a g c c c g a c g t t g g g g g g g a c g t g g g g

BsiWIBlpI 1200 chimeric intron RGSNSLVARSRTRPETRPTA c c t c c c c g a g t t g c t g a g c a c g g c c c g g c t t c g g g t g c g g g g c t c c g t a c g g g g c g t g g c g g a g g g g c t c a a c g a c t c g t g c c g g g c c g a a g c c c a c g c c c c g a g g c a t g c c c c g c a c c g

1260 chimeric intron RPEGHRAPPPPLHPHRAPRP g c g g g g c t c g c c g t g c c g g g c g g g g g g t g g c g g c a g g t g g g g g t g c c g g g c g g g g c g g g g c g c c c c g a g c g g c a c g g c c c g c c c c c c a c c g c c g t c c a c c c c c a c g g c c c g c c c c g c c c c

MreI 1320 chimeric intron RRPGPLARPLPAAAGPAGAA c c g c c t c g g g c c g g g g a g g g c t c g g g g g a g g g g c g c g g c g g c c c c c g g a g c g c c g g c g g c g g c g g a g c c c g g c c c c t c c c g a g c c c c c t c c c c g c g c c g c c g g g g g c c t c g c g g c c g c c g

1380 chimeric intron TSARRAAAM t g t c g a g g c g c g g c g a g c c g c a g c c a t t g c c t t t t a t g g t a a t c g t g c g a g a g g g c g c a g a c a g c t c c g c g c c g c t c g g c g t c g g t a a c g g a a a a t a c c a t t a g c a c g c t c t c c c g c g t c

1440 chimeric intron g g a c t t c c t t t g t c c c a a a t c t g t g c g g a g c c g a a a t c t g g g a g g c g c c g c c g c a c c c c c c c t g a a g g a a a c a g g g t t t a g a c a c g c c t c g g c t t t a g a c c c t c c g c g g c g g c g t g g g g g

1500 chimeric intron t c t a g c g g g c g c g g g g c g a a g c g g t g c g g c g c c g g c a g g a a g g a a a t g g g c g g g g a g g g c a g a t c g c c c g c g c c c c g c t t c g c c a c g c c g c g g c c g t c c t t c c t t t a c c c g c c c c t c c c g

1560 chimeric intron c t t c g t g c g t c g c c g c g c c g c c g t c c c c t t c t c c c t c t c c a g c c t c g g g g c t g t c c g c g g g a a g c a c g c a g c g g c g c g g c g g c a g g g g a a g a g g g a g a g g t c g g a g c c c c g a c a g g c g c c

1620 chimeric intron G G T T C G G C T T C T G G C G T G T G A C C pCAGGS-5 g g g g a c g g c t g c c t t c g g g g g g g a c g g g g c a g g g c g g g g t t c g g c t t c t g g c g t g t g a c c c c c c t g c c g a c g g a a g c c c c c c c t g c c c c g t c c c g c c c c a a g c c g a a g a c c g c a c a c t g g

XbaI 1680 chimeric intron MFMPSSFSYSSW g g c g g c t c t a g a g c c t c t g c t a a c c a t g t t c a t g c c t t c t t c t t t t t c c t a c a g c t c c t g c c g c c g a g a t c t c g g a g a c g a t t g g t a c a a g t a c g g a a g a a g a a a a a g g a t g t c g a g g a c

BmtINheI 1740 G C A A C G T G C T G G T T A T T G T G pCAG-F ATCWLLCCLIILAKNYSASA *QATEDNQCLIIGSAG g g c a a c g t g c t g g t t a t t g t g c t g t c t c a t c a t t t t g g c a a a g a a t t a t t c c g c t a g c g c c c g t t g c a c g a c c a a t a a c a c g a c a g a g t a g t a a a a c c g t t t c t t a a t a a g g c g a t c g c g

BsrGI 1800 1 5 10 15 MSELIKENMHMKLYMEGTV TagBFP TMSELIKENMHMKLYMEGTV GHALQNLLVHVHLQVHLAGH c a c c a t g a g c g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a c a t g g a g g g c a c c g t g t g g t a c t c g c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a t g t a c c t c c c g t g g c a

1860 20 25 30 35 DNHHFKCTSEGEGKPYEGTQ TagBFP DNHHFKCTSEGEGKPYEGTQ VVMVELACGLAFALGVLAGL g g a c a a c c a t c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c a a g c c c t a c g a g g g c a c c c a c c t g t t g g t a g t g a a g t t c a c g t g t a g g c t c c c g c t t c c g t t c g g g a t g c t c c c g t g g g t

1920 40 45 50 55 TMRIKVVEGGPLPFAFDILA TagBFP TMRIKVVEGGPLPFAFDILA GHSDLHDLAAREGEGEVDQS g a c c a t g a g a a t c a a g g t g g t c g a g g g c g g c c c t c t c c c c t t c g c c t t c g a c a t c c t g g c c t g g t a c t c t t a g t t c c a c c a g c t c c c g c c g g g a g a g g g g a a g c g g a a g c t g t a g g a c c g

PasI 1980 60 65 70 75 TSFLYGSKTFINHTQGIPDF TagBFP TSFLYGSKTFINHTQGIPDF SAEEVAALGEDVVGLADGVE t a c t a g c t t c c t c t a c g g c a g c a a g a c c t t c a t c a a c c a c a c c c a g g g c a t c c c c g a c t t a t g a t c g a a g g a g a t g c c g t c g t t c t g g a a g t a g t t g g t g t g g g t c c c g t a g g g g c t g a a

2040 80 85 90 95 FKQSFPEGFTWERVTTYEDG TagBFP FKQSFPEGFTWERVTTYEDG ELLGERLAECPLSDGCVFVP c t t c a a g c a g t c c t t c c c t g a g g g c t t c a c a t g g g a g a g a g t c a c c a c a t a c g a a g a c g g g a a g t t c g t c a g g a a g g g a c t c c c g a a g t g t a c c c t c t c t c a g t g g t g t a t g c t t c t g c c

2100 100 105 110 115 GVLTATQDTSLQDGCLIYNV TagBFP GVLTATQDTSLQDGCLIYNV AHQGSGLVGAELVAAEDVVD g g g c g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g c c t c a t c t a c a a c g t c c c g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a c g g a g t a g a t g t t g c a

2160 120 125 130 135 KIRGVNFTSNGPVMQKKTLG TagBFP KIRGVNFTSNGPVMQKKTLG LDSPHVECGVARHHLLFCEA c a a g a t c a g a g g g g t g a a c t t c a c a t c c a a c g g c c c t g t g a t g c a g a a g a a a a c a c t c g g g t t c t a g t c t c c c c a c t t g a a g t g t a g g t t g c c g g g a c a c t a c g t c t t c t t t t g t g a g c c

2220 140 145 150 155 WEAFTETLYPADGGLEGRND TagBFP WEAFTETLYPADGGLEGRND PLGEGLRQVGSVAAQFASVV c t g g g a g g c c t t c a c c g a g a c g c t g t a c c c c g c t g a c g g c g g c c t g g a a g g c a g a a a c g a g a c c c t c c g g a a g t g g c t c t g c g a c a t g g g g c g a c t g c c g c c g g a c c t t c c g t c t t t g c t

2280 160 165 170 175 MALKLVGGSHLIANIKTTYR TagBFP MALKLVGGSHLIANIKTTYR HGQLEHAPAM c a t g g c c c t g a a g c t c g t g g g c g g g a g c c a t c t g a t c g c a a a c a t c a a g a c c a c a t a t a g g t a c c g g g a c t t c g a g c a c c c g c c c t c g g t a g a c t a g c g t t t g t a g t t c t g g t g t a t a t c

2340 180 185 190 195 SKKPAKNLKMPGVYYVDYRL TagBFP SKKPAKNLKMPGVYYVDYRL a t c c a a g a a a c c c g c t a a g a a c c t c a a g a t g c c t g g c g t c t a c t a t g t g g a c t a c a g a c t t a g g t t c t t t g g g c g a t t c t t g g a g t t c t a c g g a c c g c a g a t g a t a c a c c t g a t g t c t g a

MscI 2400 200 205 210 215 ERIKEANNETYVEQHEVAVA TagBFP ERIKEANNETYVEQHEVAVA g g a a a g a a t c a a g g a g g c c a a c a a c g a g a c c t a c g t c g a g c a g c a c g a g g t g g c a g t g g c c c t t t c t t a g t t c c t c c g g t t g t t g c t c t g g a t g c a g c t c g t c g t g c t c c a c c g t c a c c g

2460 220 225 230 RYCDLPSKLGHKLN (in frame with TagBFP) SGLRSR TagBFP RYCDLPSKLGHKLNSGLRSR c a g a t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a a g c t t a a t t c c g g a c t c a g a t c t c g g t c t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t t c g a a t t a a g g c c t g a g t c t a g a g c

2520 (in frame with TagBFP) VATVKFKYKGEEKQVDISKI VATVKFKYKGEEKQVDISKI a g t g g c a a c a g t c a a g t t c a a a t a c a a g g g g g a g g a a a a g c a g g t g g a c a t t a g t a a g a t t c a c c g t t g t c a g t t c a a g t t t a t g t t c c c c c t c c t t t t c g t c c a c c t g t a a t c a t t c t a

2580 (in frame with TagBFP) LSVGRYGKLIHFLYDLGGGK LSVGRYGKLIHFLYDLGGGK t c t g a g c g t c g g a a g a t a c g g g a a g c t g a t c c a c t t c c t g t a c g a c c t g g g a g g a g g c a a a g a c t c g c a g c c t t c t a t g c c c t t c g a c t a g g t g a a g g a c a t g c t g g a c c c t c c t c c g t t

2640 (in frame with TagBFP) AGMGMVSEKDAPKELLQMLE AGMGMVSEKDAPKELLQMLE g g c a g g a a t g g g c a t g g t g a g c g a g a a g g a t g c c c c c a a g g a g c t g c t c c a g a t g c t g g a c c g t c c t t a c c c g t a c c a c t c g c t c t t c c t a c g g g g g t t c c t c g a c g a g g t c t a c g a c c t

SalIBamHI 2700 (in frame with TagBFP) KQKK* KQKK* g a a g c a g a a g a a g t a a g g a t c c a c t a g t g t c g a c c t c g a g a c c t a g a a a a a c a t g g a g c a c t t c g t c t t c t t c a t t c c t a g g t g a t c a c a g c t g g a g c t c t g g a t c t t t t t g t a c c t c g t

2760 a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c a a g a g t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c g t g t t c t c

Acc65IKpnI 2820 g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a c t t a c a a g c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a a t g t t c

2880 g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g

2940 t c c c a a c g a a g a c a a a a t c g t c g a g a g a t g c t g c a t a t a a g c a g c t g c t t t t t g c t t g t a a g g g t t g c t t c t g t t t t a g c a g c t c t c t a c g a c g t a t a t t c g t c g a c g a a a a a c g a a c a t

3000 5' LTR (truncated) c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g

3060 5' LTR (truncated) c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a

3120 5' LTR (truncated) t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t

3180 5' LTR (truncated) g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a

3240 c a g a g a g t g a g a g g c c t t g a c a t t a t a a t a g a t t t a g c a g g a a t t g a a c t a g g a g t g g a g g t c t c t c a c t c t c c g g a a c t g t a a t a t t a t c t a a a t c g t c c t t a a c t t g a t c c t c a c c t c

3300 c a c a c a g g c a a a g c t g c a g a a g t a c t t g g a a g a a g c c a c c a g a g a t a c t c a c g a t t c t g c g t g t g t c c g t t t c g a c g t c t t c a t g a a c c t t c t t c g g t g g t c t c t a t g a g t g c t a a g a c g

3360 a c a t a c c t g g c t a a t c c c a g a t c c t a a g g a t t a c a t t a a g t t t a c t a a c a t t t a t a t a a t t g t a t g g a c c g a t t a g g g t c t a g g a t t c c t a a t g t a a t t c a a a t g a t t g t a a a t a t a t t a

PacI 3420 g a t t t a t a g t t t a a a g t a t a a a c t t a t c t a a t t t a c t a t t c t g a c a g a t a t t a a t t a a t c c t a a a t a t c a a a t t t c a t a t t t g a a t a g a t t a a a t g a t a a g a c t g t c t a t a a t t a a t t a g

3480 c t c a a a t a t c a t a a g a g a t g a t t a c t a t t a t c c c c a t t t a a c a c a a g a g g a a a c t g a g a g g a g t t t a t a g t a t t c t c t a c t a a t g a t a a t a g g g g t a a a t t g t g t t c t c c t t t g a c t c t c

3540 g g a a a g a t g t t g a a g t a a t t t t c c c a c a a t t a c a g c a t c c g t t a g t t a c g a c t c t a t g a t c c t t t c t a c a a c t t c a t t a a a a g g g t g t t a a t g t c g t a g g c a a t c a a t g c t g a g a t a c t a

Tth111IPflFI 3600 c t t c t g a c a c a a a t t c c a t t t a c t c c t c a c c c t a t g a c t c a g t c g a a t a t a t c a a a g t t a g a a g a c t g t g t t t a a g g t a a a t g a g g a g t g g g a t a c t g a g t c a g c t t a t a t a g t t t c a a t

3660 t g g a c a t t a t g c t a a g t a a c a a a t t a c c c t t t t a t a t a g t a a a t a c t g a g t a g a t t g a g a a c c t g t a a t a c g a t t c a t t g t t t a a t g g g a a a a t a t a t c a t t t a t g a c t c a t c t a a c t c t

3720 g a a g a a a t t g t t t g c a a a c c t g a a t a g c t t c a a g a a g a a g a g a a g t g a g g a t a a g a a t a a c t t c t t t a a c a a a c g t t t g g a c t t a t c g a a g t t c t t c t t c t c t t c a c t c c t a t t c t t a t t

3780 c a g t t g t c a t t t a a c a a g t t t t a a c a a g t a a c t t g g t t a g a a a g g g a t t c a a a t g c a t a a g t c a a c a g t a a a t t g t t c a a a a t t g t t c a t t g a a c c a a t c t t t c c c t a a g t t t a c g t a t t

3840 a g c a a g g g a t a a a t t t t t c t g g c a a c a a g a c t a t a c a a t a t a a c c t t a a a t a t g a c t t c a t c g t t c c c t a t t t a a a a a g a c c g t t g t t c t g a t a t g t t a t a t t g g a a t t t a t a c t g a a g t

3900 a a t a a t t g t t g g a a c t t g a t a a a a c t a a t t a a a t a t t a t t g a a g a t t a t c a a t a t t a t a a t t a t t a a c a a c c t t g a a c t a t t t t g a t t a a t t t a t a a t a a c t t c t a a t a g t t a t a a t a t t

3960 a t g t a a t t t a c t t t t a a a a a g g g a a c a t a g a a a t g t g t a t c a t t a g a g t a g a a a a c a a t c t a c a t t a a a t g a a a a t t t t t c c c t t g t a t c t t t a c a c a t a g t a a t c t c a t c t t t t g t t a g

4020 c t t a t t a t c a c a a t t t g t c a a a a c a a g t t t g t t a t t a a c a c a a g t a g a a t a c t g c a t t c a g a a t a a t a g t g t t a a a c a g t t t t g t t c a a a c a a t a a t t g t g t t c a t c t t a t g a c g t a a g t

4080 a t t a a g t t g a c t g c a g a t t t t g t g t t t t g t t a a a a t t a g a a a g a g a t a a c a a c a a t t t g a t a a t t c a a c t g a c g t c t a a a a c a c a a a a c a a t t t t a a t c t t t c t c t a t t g t t g t t a a a c t

4140 a t t a t t g a a a g t a a c a t g t a a a t a g t t c t a c a t a c g t t c t t t t g a c a t c t t g t t c a a t c a t a a t a a c t t t c a t t g t a c a t t t a t c a a g a t g t a t g c a a g a a a a c t g t a g a a c a a g t t a g t

4200 t t g a t c g a a g t t c t t t a t c t t g g a a g a a t t t g t t c c a a a g a c t c t g a a a t a a g g a a a a c a a a c t a g c t t c a a g a a a t a g a a c c t t c t t a a a c a a g g t t t c t g a g a c t t t a t t c c t t t t g t

4260 a t c t a t t a t a t a g t c t c a c a c c t t t g t t t t a c t t t t a g t g a t t t c a a t t t a a t a a t g t a a t a g a t a a t a t a t c a g a g t g t g g a a a c a a a a t g a a a a t c a c t a a a g t t a a a t t a t t a c a t t

4320 a t g g t t a a a a t t t a t t c t t c t c t g a g a t c a t t t c a c a t t g c a g a t a g a a a a c c t g a g a c t t a c c a a t t t t a a a t a a g a a g a g a c t c t a g t a a a g t g t a a c g t c t a t c t t t t g g a c t c t g a

4380 g g g g t a a t t t t t a t t a a a a t c t a a t t t a a t c t c a g a a a c a c a t c t t t a t t c t a a c a t c a a c c c c a t t a a a a a t a a t t t t a g a t t a a a t t a g a g t c t t t g t g t a g a a a t a a g a t t g t a g t t

4440 t t t t t c c a g t t t g a t a t t a t c a t a t a a a g t c a g c c t t c c t c a t c t g c a g g t t c c a c a a c a a a a a a g g t c a a a c t a t a a t a g t a t a t t t c a g t c g g a a g g a g t a g a c g t c c a a g g t g t t g t

4500 a a a a t c c a a c c a a c t g t g g a t c a a a a a t a t t g g g a a a a a a t t a a a a a t a g c a a t a c a a c a t t t t a g g t t g g t t g a c a c c t a g t t t t t a t a a c c c t t t t t t a a t t t t t a t c g t t a t g t t g t

4560 a t a a a a a a a t a c a a a t c a g a a a a a c a g c a c a g t a t a a c a a c t t t a t t t a g c a t t t a c a a t t a t t t t t t t a t g t t t a g t c t t t t t g t c g t g t c a t a t t g t t g a a a t a a a t c g t a a a t g t t a

4620 c t a t t a g g t a t t a t a a g t a a t c t a g c c a g a t c c t c t a c g c c g g a c g c a t c g t g g c c g g c a g a t a a t c c a t a a t a t t c a t t a g a t c g g t c t a g g a g a t g c g g c c t g c g t a g c a c c g g c c g t

4680 G G A T A T A G C G G C T G T A G T G G pBRrevBam t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g a c a t c a c c g a t g g g g a a g a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c t g t a g t g g c t a c c c c t t c

4740 a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t t g t t t c g g c g t g g g t a t g g t g g c a g g c c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a a c a a a g c c g c a c c c a t a c c a c c g t c c g g

4800 c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c t t g c a t g c a c c a t t c c t t g c g g c g g c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g a a c g t a c g t g g t a a g g a a c g c c g c c g c c

4860 t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c t t c c t a a t g c a g g a g t c g c a t a a g g g a g a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g a a g g a t t a c g t c c t c a g c g t a t t c c c t c

4920 A T G T T A G A C G A G A C T A C G G C pRS-marker a g c g t c g a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c t c g c a g c t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g

4980 c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a

5040 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t

5100 C G G A G C A C T A T G C G G A T A A pBRforEco c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c

5160 AmpR promoter a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a

5220 AmpR promoter a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t

5280 1 5 signal sequence MSIQHFRVA AmpR promoter AmpR MSIQHFRVA t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g

5340 10 15 20 signal sequence LIPFFAAFCLPVFA 25 HPETLV AmpR LIPFFAAFCLPVFAHPETLV c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c

5400 30 35 40 45 KVKDAEDQLGARVGYIELDL AmpR KVKDAEDQLGARVGYIELDL a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g

5460 50 55 60 65 NSGKILESFRPEERFPMMST AmpR NSGKILESFRPEERFPMMST a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a

5520 70 75 80 85 FKVLLCGAVLSRIDAGQEQL AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FKVLLCGAVLSRIDAGQEQL t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g

5580 90 95 100 105 GRRIHYSQNDLVEYSPVTEK AmpR GRRIHYSQNDLVEYSPVTEK g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c

5640 110 115 120 125 HLTDGMTVRELCSAAITMSD AmpR HLTDGMTVRELCSAAITMSD c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a

PvuI 5700 130 135 140 145 NTAANLLLTTIGGPKELTAF AmpR NTAANLLLTTIGGPKELTAF a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a

5760 150 155 160 165 LHNMGDHVTRLDRWEPELNE AmpR LHNMGDHVTRLDRWEPELNE t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t

FspI 5820 170 175 180 185 AIPNDERDTTMPVAMATTLR AmpR AIPNDERDTTMPVAMATTLR g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g

5880 190 195 200 205 KLLTGELLTLASRQQLIDWM AmpR KLLTGELLTLASRQQLIDWM a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c

5940 210 215 220 225 EADKVAGPLLRSALPAGWFI AmpR EADKVAGPLLRSALPAGWFI g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a

6000 230 235 240 245 ADKSGAGERGSRGIIAALGP AmpR ADKSGAGERGSRGIIAALGP g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t

AhdI 6060 250 255 260 265 DGKPSRIVVIYTTGSQATMD AmpR DGKPSRIVVIYTTGSQATMD g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a

6120 270 275 280 285 ERNRQIAEIGASLIKHW* AmpR ERNRQIAEIGASLIKHW* g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t

6180 g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c

6240 a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c

6300 ori t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a

6360 ori c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c

6420 ori c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t

6480 ori c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t

6540 ori c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c

6600 ori t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g

6660 ori t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t

6720 ori t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c

6780 ori G G G A A A C pBR322ori-F t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g

6840 ori G C C T G G T A T C T T T pBR322ori-F g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c

6900 ori t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c

6960 t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a

7020 g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g

7080 A G C G A G T C A G T G A G C G A G L4440 g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g

7140 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t

7200 SV40 promoter a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t

7260 SV40 promoter c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t

7320 SV40 promoter SV40 ori a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

7380 SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

AvrIISfiI 7440 SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

7500 SV40 promoter t t t g c a a a a a g c t t g g a c a c a a g a c a g g c t t g c g a g a t a t g t t t g a g a a t a c c a c t t t a t a a a c g t t t t t c g a a c c t g t g t t c t g t c c g a a c g c t c t a t a c a a a c t c t t a t g g t g a a a t a

7560 c c c g c g t c a g g g a g a g g c a g t g c g t a a a a a g a c g c g g a c t c a t g t g a a a t a c t g g t t t t t g g g c g c a g t c c c t c t c c g t c a c g c a t t t t t c t g c g c c t g a g t a c a c t t t a t g a c c a a a a a

7620 a g t g c g c c a g a t c t c t a t a a t c t c g c g c a a c c t a t t t t c c c c t c g a a c a c t t t t t a a g c c t c a c g c g g t c t a g a g a t a t t a g a g c g c g t t g g a t a a a a g g g g a g c t t g t g a a a a a t t c g g

7680 g t a g a t a a a c a g g c t g g g a c a c t t c a c a t g a g c g a a a a a t a c a t c g t c a c c t g g g a c a t g c a t c t a t t t g t c c g a c c c t g t g a a g t g t a c t c g c t t t t t a t g t a g c a g t g g a c c c t g t a c

7740 t t g c a g a t c c a t g c a c g t a a a c t c g c a a g c c g a c t g a t g c c t t c t g a a c a a t g g a a a g g c a a c g t c t a g g t a c g t g c a t t t g a g c g t t c g g c t g a c t a c g g a a g a c t t g t t a c c t t t c c g

7800 a t t a t t g c c g t a a g c c g t g g c g g t c t g t a c c g g g t g c g t t a c t g g c g c g t g a a c t g g g t a t a a t a a c g g c a t t c g g c a c c g c c a g a c a t g g c c c a c g c a a t g a c c g c g c a c t t g a c c c a t

7860 t t c g t c a t g t c g a t a c c g t t t g t a t t t c c a g c t a c g a t c a c g a c a a c c a g c g c g a g c t t a a a g c a g t a c a g c t a t g g c a a a c a t a a a g g t c g a t g c t a g t g c t g t t g g t c g c g c t c g a a t

7920 a a g t g c t g a a a c g c g c a g a a g g c g a t g g c g a a g g c t t c a t c g t t a t t g a t g a c c t g g t g g t t c a c g a c t t t g c g c g t c t t c c g c t a c c g c t t c c g a a g t a g c a a t a a c t a c t g g a c c a c c

AgeI 7980 a t a c c g g t g g t a c t g c g g t t g c g a t t c g t g a a a t g t a t c c a a a a g c g c a c t t t g t c a c c a t a t g g c c a c c a t g a c g c c a a c g c t a a g c a c t t t a c a t a g g t t t t c g c g t g a a a c a g t g g t

8040 t c t t c g c a a a a c c g g c t g g t c g t c c g c t g g t t g a t g a c t a t g t t g t t g a t a t c c c g c a a g a g a a g c g t t t t g g c c g a c c a g c a g g c g a c c a a c t a c t g a t a c a a c a a c t a t a g g g c g t t c

8100 a t a c c t g g a t t g a a c a g c c g t g g g a t a t g g g c g t c g t a t t c g t c c c g c c a a t c t c c g g t c t a t g g a c c t a a c t t g t c g g c a c c c t a t a c c c g c a g c a t a a g c a g g g c g g t t a g a g g c c a g

8160 g c t a a t c t t t t c a a c g c c t g g c a c t g c c g g g c g t t g t t c t t t t t a a c t t c a g g c g g g t t a c g a t t a g a a a a g t t g c g g a c c g t g a c g g c c c g c a a c a a g a a a a a t t g a a g t c c g c c c a a t

8220 c a a t a g t t t c c a g t a a g t a t t c t g g a g g c t g c a t c c a t g a c a c a g g c a a a c c t g a g c g a a g t t a t c a a a g g t c a t t c a t a a g a c c t c c g a c g t a g g t a c t g t g t c c g t t t g g a c t c g c t t

8280 a c c c t g t t c a a a c c c c g c t t t a a a c a t c c t g a a a c c t c g a c g c t a g t c c g c c g c t t t a a t t g g g a c a a g t t t g g g g c g a a a t t t g t a g g a c t t t g g a g c t g c g a t c a g g c g g c g a a a t t a

8340 c a c g g c g c a c a a c c g c c t g t g c a g t c g g c c c t t g a t g g t a a a a c c a t c c c t c a c t g g t a t g t g c c g c g t g t t g g c g g a c a c g t c a g c c g g g a a c t a c c a t t t t g g t a g g g a g t g a c c a t a

8400 c g c a t g a t t a a c c g t c t g a t g t g g a t c t g g c g c g g c a t t g a c c c a c g c g a a a t c c t c g a c g c g t a c t a a t t g g c a g a c t a c a c c t a g a c c g c g c c g t a a c t g g g t g c g c t t t a g g a g c t g

8460 g t c c a g g c a c g t a t t g t g a t g a g c g a t g c c g a a c g t a c c g a c g a t g a t t t a t a c g a t a c g c a g g t c c g t g c a t a a c a c t a c t c g c t a c g g c t t g c a t g g c t g c t a c t a a a t a t g c t a t g c

8520 g t g a t t g g c t a c c g t g g c g g c a a c t g g a t t t a t g a g t g g g c c c c g g a t c t t t g t g a a g g a c a c t a a c c g a t g g c a c c g c c g t t g a c c t a a a t a c t c a c c c g g g g c c t a g a a a c a c t t c c t

8580 a c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c a a a c t a c c t a c a g a g a t t t a a a g c t c t a a t g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g t t t g a t g g a t g t c t c t a a a t t t c g a g a t t

8640 small t intron g g y w r w t r t r w r a y w t w w w w g k r y a w a m t r y s t a c a g a g a t t t a a a g c t c t a a g g t a a a t c c r w y w a y a y w y t r w a w w w w c m y r t w t k a y r s a t g t c t c t a a a t t t c g a g a t t c c a t t t a

8700 small t intron a t a a a a t t t t t a a g t g t a t a a t g t g t t a a a c t a c t g a t t c t a a t t g t t t g t g t a t t t t a g t a t t t t a a a a a t t c a c a t a t t a c a c a a t t t g a t g a c t a a g a t t a a c a a a c a c a t a a a a t c

8760 a t t c c a a c c t a t g g a a c t g a t g a a t g g g a g c a g t g g t g g a a t g c c t t t a a t g a g g a a a a c t a a g g t t g g a t a c c t t g a c t a c t t a c c c t c g t c a c c a c c t t a c g g a a a t t a c t c c t t t t g

8820 MPSSDDEATADSQH c t g t t t t g c t c a g a a g a a a t g c c a t c t a g t g a t g a t g a g g c t a c t g c t g a c t c t c a a c a t g a c a a a a c g a g t c t t c t t t a c g g t a g a t c a c t a c t a c t c c g a t g a c g a c t g a g a g t t g t a

8880 130 PKKKRKV (in frame with SV40 NLS) EDPKDFPSEL SV40 NLS STPPKKKRKVEDPKDFPSEL t c t a c t c c t c c a a a a a a g a a g a g a a a g g t a g a a g a c c c c a a g g a c t t t c c t t c a g a a t t g a g a t g a g g a g g t t t t t t c t t c t c t t t c c a t c t t c t g g g g t t c c t g a a a g g a a g t c t t a a c

8940 (in frame with SV40 NLS) LSFLSHAVFSNRTLACFAIY LSFLSHAVFSNRTLACFAIY c t a a g t t t t t t g a g t c a t g c t g t g t t t a g t a a t a g a a c t c t t g c t t g c t t t g c t a t t t a c g a t t c a a a a a a c t c a g t a c g a c a c a a a t c a t t a t c t t g a g a a c g a a c g a a a c g a t a a a t g

9000 (in frame with SV40 NLS) TTKEKAALLYKKIMEKYSVT TTKEKAALLYKKIMEKYSVT a c c a c a a a g g a a a a a g c t g c a c t g c t a t a c a a g a a a a t t a t g g a a a a a t a t t c t g t a a c c t g g t g t t t c c t t t t t c g a c g t g a c g a t a t g t t c t t t t a a t a c c t t t t t a t a a g a c a t t g g

9060 (in frame with SV40 NLS) FISRHNSYNHNILFFLTPHR FISRHNSYNHNILFFLTPHR t t t a t a a g t a g g c a t a a c a g t t a t a a t c a t a a c a t a c t g t t t t t t c t t a c t c c a c a c a g g a a a t a t t c a t c c g t a t t g t c a a t a t t a g t a t t g t a t g a c a a a a a a g a a t g a g g t g t g t c c

9120 (in frame with SV40 NLS) HRVSAINNYAQKLCTFSFLI HRVSAINNYAQKLCTFSFLI c a t a g a g t g t c t g c t a t t a a t a a c t a t g c t c a a a a a t t g t g t a c c t t t a g c t t t t t a a t t g t a t c t c a c a g a c g a t a a t t a t t g a t a c g a g t t t t t a a c a c a t g g a a a t c g a a a a a t t a a

9180 (in frame with SV40 NLS) CKGVNKEYLMYSALTRDHNQ CKGVNKEYLMYSALTRDHNQ t g t a a a g g g g t t a a t a a g g a a t a t t t g a t g t a t a g t g c c t t g a c t a g a g a t c a t a a t c a g a c a t t t c c c c a a t t a t t c c t t a t a a a c t a c a t a t c a c g g a a c t g a t c t c t a g t a t t a g t c

9240 (in frame with SV40 NLS) PYHICRGFTCFKKPPTPPPE PYHICRGFTCFKKPPTPPPE c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t

HpaI 9300 PET* SV40 poly(A) signal PET* c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c

9360 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g

9420 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a c t g g a t a a c a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t g a c c t a t t g

9480 t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c c a t a c c c t a t t a c c a c t g c c a a t t a a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g g t a t g g g a t a a t g g t g a c g g t t a a t

9540 c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t c t g a a t t a t t t t c a t t t t a a a g a a a g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a g a c t t a a t a a a a g t a a a a t t t c t t t

9600 3' LTR t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g t t g g a a g g g c t a a t t c a c t c c c a a a a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c a a c c t t c c c g a t t a a g t g a g g g t t t

9660 3' LTR g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t t c c c t g a t t a g c c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g a a g g g a c t a a t c g

9720 3' LTR a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c t g a c c t t t g g a t g g t g c t a c a a g c t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g a c t g g a a a c c t a c c a c g a t g t t c g

9780 3' LTR t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a t a a a g g a g a g a a c a c c a g c t t g t a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t t a t t t c c t c t c t t g t g g t c g a a c a

9840 3' LTR t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t g t t a g a g t g g a g g t a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c a c a a t c t c a c c t c c a

PmlI 9900 3' LTR t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g a g c t g c a t c c g g a g t a c t t c a a g a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c t c g a c g t a g g c c t c a t g a a g t t c t

9960 3' LTR a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g c t g g g g a c t t t c c a g g g a g g c g t g t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c g a c c c c t g a a a g g t c c c t c c g c a c

10,020 3' LTR g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t c c t g c a t a t a a g c a g c t g c t t t t t c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a g g a c g t a t a t t c g t c g a c g a a a a a

10,080 3' LTR g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t

10,140 3' LTR g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g

10,200 3' LTR c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t

10,260 3' LTR HIV-1 Ψ a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a

10,320 HIV-1 Ψ c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c

10,380 HIV-1 Ψ t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c

NruI* 10,440 HIV-1 Ψ t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c

10,500 g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c

10,560 c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g

10,620 a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g

10,680 t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c

NotI 10,740 a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a

10,800 MRDNWRSELYKYKVVK g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t

10,860 IEPLGVAPTKAKRRVVQREK a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t

10,920 RRE RAVGIGALFLGFLGAAGSTM a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c

10,980 RRE GAASMTLTVQARQLLSGIVQ g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c

BbvCI 11,040 RRE QQNNLLRAIEAQQHLLQLTV c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g

11,100 RRE WGIKQLQARILAVERYLKDQ t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t

AleI 11,160 RRE QLLGIWGCSGKLICTTAVPW c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c

11,220 NASWSNKSLEQIWNHTTWME a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c

11,280 WDREINNYTSLIHSLIEESQ t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t

11,340 1 5 10 15 KNEQELLELDKWASL W gp41 peptide NQQEKNEQELLELDKWASLW a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c

11,400 (in frame with gp41 peptide) NWFNITNWLWYIKLFIMIVG NWFNITNWLWYIKLFIMIVG a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t

11,460 (in frame with gp41 peptide) GLVGLRIVFAVLSIVNRVRQ GLVGLRIVFAVLSIVNRVRQ g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c

PpuMIKflI 11,520 (in frame with gp41 peptide) GYSPLSFQTHLPTPRGPDRP GYSPLSFQTHLPTPRGPDRP g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g

11,580 (in frame with gp41 peptide) EGIEEEGGERDRDRSIRLVN EGIEEEGGERDRDRSIRLVN g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g

EcoRI 11,640 (in frame with gp41 peptide) GSRRYRRIHKWQYSSTILKE cPPT/CTS GSRRYRRIHKWQYSSTILKE g g a t c t c g a c g g t a t c g c c g a a t t c a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a c c t a g a g c t g c c a t a g c g g c t t a a g t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t

11,700 (in frame with gp41 peptide) KGGLGGTVQGKE* cPPT/CTS KGGLGGTVQGKE* a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t

11,760 cPPT/CTS t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t

MluI 3ʹ 5ʹ 11,795 g g g a c a g c a g a g a t c c a c t t t g g c t g a t a c g c g t t c c c t g t c g t c t c t a g g t g a a a c c g a c t a t g c g c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.