Addgene pCMV-dLbCpf1-BE-YE2 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCMV-dLbCpf1-BE-YE2 Sequencing Result


Map View

SpeI (7932) MluI (7911) NruI (7891) MfeI (7844) pRS-marker (7727 .. 7746) BspDI * - ClaI * (7694) SgrDI (7677) AmpR promoter SspI (7560) Amp-R (7306 .. 7325) PvuI (7126) AhdI (6756) pBR322ori-F (6004 .. 6023) L4440 (5753 .. 5770) CAP binding site lac promoter M13/pUC Reverse (5535 .. 5557) lac operator M13 Reverse (5522 .. 5538) SphI (5427) bGH poly(A) signal BGH-rev (5221 .. 5238) BclI * (5214) PmeI (5205) 6xHis SV40 NLS SV40 NLS AfeI (4446) NdeI (1) SnaBI (107) NcoI (127) CMV promoter CMV-F (286 .. 306) EagI - NotI - SacII (370) T7 (377 .. 396) T7 promoter SV40 NLS BsmBI - Esp3I (468) NgoMIV (569) NaeI (571) DraIII (674) CsiI - SexAI * (685) EcoRI (736) StuI * (821) TspMI - XmaI (1135) SmaI (1137) BspEI (1409) RsrII (1424) Bsu36I (1468) BstZ17I (2048) PstI (2369) NsiI (3123) PaqCI (3978) pCMV-dLbCpf1-BE-YE2 8166 bp

Sequence View

NdeI 5ʹ 3ʹ 60 CMV enhancer a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c

SnaBI 120 CMV enhancer c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c

NcoI 180 CMV enhancer CMV promoter c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a

240 CMV promoter c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t

300 CMV promoter C G C A A A T G G G C G G T A CMV-F a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t

360 CMV promoter G G C G T G CMV-F g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a

SacIIEagINotI 420 PKK T7 promoter SV40 NLS T A A T A C G A C T C A C T A T A G G G T7 MPKK a g a g a t c c g c g g c c g c t a a t a c g a c t c a c t a t a g g g a g a g c c g c c a c c a t g c c c a a g a a g t c t c t a g g c g c c g g c g a t t a t g c t g a g t g a t a t c c c t c t c g g c g g t g g t a c g g g t t c t t c

BsmBIEsp3I 480 130 KRKV (in frame with SV40 NLS) MSSETGPVAVDPTLRR SV40 NLS KRKVMSSETGPVAVDPTLRR a a g a g g a a a g t c a t g a g c t c a g a g a c t g g c c c a g t g g c t g t g g a c c c c a c a t t g a g a c g g t t c t c c t t t c a g t a c t c g a g t c t c t g a c c g g g t c a c c g a c a c c t g g g g t g t a a c t c t g c c

540 (in frame with SV40 NLS) RIEPHEFEVFFDPRELRKET RIEPHEFEVFFDPRELRKET c g g a t c g a g c c c c a t g a g t t t g a g g t a t t c t t c g a t c c g a g a g a g c t c c g c a a g g a g a c c g c c t a g c t c g g g g t a c t c a a a c t c c a t a a g a a g c t a g g c t c t c t c g a g g c g t t c c t c t g g

NgoMIVNaeI 600 (in frame with SV40 NLS) CLLYEINWGGRHSIWRHTSQ CLLYEINWGGRHSIWRHTSQ t g c c t g c t t t a c g a a a t t a a t t g g g g g g g c c g g c a c t c c a t t t g g c g a c a t a c a t c a c a g a c g g a c g a a a t g c t t t a a t t a a c c c c c c c g g c c g t g a g g t a a a c c g c t g t a t g t a g t g t c

660 (in frame with SV40 NLS) NTNKHVEVNFIEKFTTERYF NTNKHVEVNFIEKFTTERYF a a c a c t a a c a a g c a c g t c g a a g t c a a c t t c a t c g a g a a g t t c a c g a c a g a a a g a t a t t t c t t g t g a t t g t t c g t g c a g c t t c a g t t g a a g t a g c t c t t c a a g t g c t g t c t t t c t a t a a a g

SexAI*CsiIDraIII 720 (in frame with SV40 NLS) CPNTRCSITWFLSYSPCGEC CPNTRCSITWFLSYSPCGEC t g t c c g a a c a c a a g g t g c a g c a t t a c c t g g t t t c t c a g c t a c a g c c c a t g c g g c g a a t g t a c a g g c t t g t g t t c c a c g t c g t a a t g g a c c a a a g a g t c g a t g t c g g g t a c g c c g c t t a c a

EcoRI 780 (in frame with SV40 NLS) SRAITEFLSRYPHVTLFIYI SRAITEFLSRYPHVTLFIYI a g t a g g g c c a t c a c t g a a t t c c t g t c a a g g t a t c c c c a c g t c a c t c t g t t t a t t t a c a t c t c a t c c c g g t a g t g a c t t a a g g a c a g t t c c a t a g g g g t g c a g t g a g a c a a a t a a a t g t a g

StuI* 840 (in frame with SV40 NLS) ARLYHHADPRNRQGLEDLIS ARLYHHADPRNRQGLEDLIS g c a a g g c t g t a c c a c c a c g c t g a c c c c c g c a a t c g a c a a g g c c t g g a a g a t t t g a t c t c t c g t t c c g a c a t g g t g g t g c g a c t g g g g g c g t t a g c t g t t c c g g a c c t t c t a a a c t a g a g a

900 (in frame with SV40 NLS) SGVTIQIMTEQESGYCWRNF SGVTIQIMTEQESGYCWRNF t c a g g t g t g a c t a t c c a a a t t a t g a c t g a g c a g g a g t c a g g a t a c t g c t g g a g a a a c t t t a g t c c a c a c t g a t a g g t t t a a t a c t g a c t c g t c c t c a g t c c t a t g a c g a c c t c t t t g a a a

960 (in frame with SV40 NLS) VNYSPSNEAHWPRYPHLWVR VNYSPSNEAHWPRYPHLWVR g t g a a t t a t a g c c c g a g t a a t g a a g c c c a c t g g c c t a g g t a t c c c c a t c t g t g g g t a c g a c a c t t a a t a t c g g g c t c a t t a c t t c g g g t g a c c g g a t c c a t a g g g g t a g a c a c c c a t g c t

1020 (in frame with SV40 NLS) LYVLELYCIILGLPPCLNIL LYVLELYCIILGLPPCLNIL c t g t a c g t t c t t g a a c t g t a c t g c a t c a t a c t g g g c c t g c c t c c t t g t c t c a a c a t t c t g g a c a t g c a a g a a c t t g a c a t g a c g t a g t a t g a c c c g g a c g g a g g a a c a g a g t t g t a a g a c

1080 (in frame with SV40 NLS) RRKQPQLTFFTIALQSCHYQ RRKQPQLTFFTIALQSCHYQ a g a a g g a a g c a g c c a c a g c t g a c a t t c t t t a c c a t c g c t c t t c a g t c t t g t c a t t a c c a g t c t t c c t t c g t c g g t g t c g a c t g t a a g a a a t g g t a g c g a g a a g t c a g a a c a g t a a t g g t c

TspMIXmaISmaI 1140 (in frame with SV40 NLS) RLPPHILWATGLKSGSETPG RLPPHILWATGLKSGSETPG c g a c t g c c c c c a c a c a t t c t c t g g g c c a c c g g g t t g a a a a g c g g c a g c g a g a c t c c c g g g g c t g a c g g g g g t g t g t a a g a g a c c c g g t g g c c c a a c t t t t c g c c g t c g c t c t g a g g g c c c

1200 (in frame with SV40 NLS) TSESATPESMSKLEKFTNCY TSESATPESMSKLEKFTNCY a c c t c a g a g t c c g c c a c a c c c g a a a g t a t g t c a a a g c t g g a g a a a t t c a c c a a c t g t t a t t g g a g t c t c a g g c g g t g t g g g c t t t c a t a c a g t t t c g a c c t c t t t a a g t g g t t g a c a a t a

1260 (in frame with SV40 NLS) SLSKTLRFKAIPVGKTQENI SLSKTLRFKAIPVGKTQENI a g c c t g t c t a a g a c c c t g c g c t t c a a g g c a a t c c c a g t g g g c a a g a c a c a a g a g a a c a t t t c g g a c a g a t t c t g g g a c g c g a a g t t c c g t t a g g g t c a c c c g t t c t g t g t t c t c t t g t a a

1320 (in frame with SV40 NLS) DNKRLLVEDEKRAEDYKGVK DNKRLLVEDEKRAEDYKGVK g a c a a c a a a c g g c t c c t g g t g g a g g a t g a g a a g a g g g c t g a a g a t t a c a a g g g c g t t a a g c t g t t g t t t g c c g a g g a c c a c c t c c t a c t c t t c t c c c g a c t t c t a a t g t t c c c g c a a t t c

1380 (in frame with SV40 NLS) KLLDRYYLSFINDVLHSIKL KLLDRYYLSFINDVLHSIKL a a g c t g c t g g a t a g g t a c t a t c t g t c a t t c a t c a a c g a t g t c c t c c a c a g t a t c a a g c t g t t c g a c g a c c t a t c c a t g a t a g a c a g t a a g t a g t t g c t a c a g g a g g t g t c a t a g t t c g a c

RsrIIBspEI 1440 (in frame with SV40 NLS) KNLNNYISLFRKKTRTEKEN KNLNNYISLFRKKTRTEKEN a a g a a t c t g a a c a a t t a c a t t t c t c t g t t c c g g a a g a a g a c a c g g a c c g a g a a g g a g a a c t t c t t a g a c t t g t t a a t g t a a a g a g a c a a g g c c t t c t t c t g t g c c t g g c t c t t c c t c t t g

Bsu36I 1500 (in frame with SV40 NLS) KELENLEINLRKEIAKAFKG KELENLEINLRKEIAKAFKG a a a g a g c t g g a g a a t c t g g a g a t c a a c c t g a g g a a a g a a a t a g c t a a g g c t t t c a a a g g g t t t c t c g a c c t c t t a g a c c t c t a g t t g g a c t c c t t t c t t t a t c g a t t c c g a a a g t t t c c c

1560 (in frame with SV40 NLS) NEGYKSLFKKDIIETILPEF NEGYKSLFKKDIIETILPEF a a c g a g g g t t a c a a g t c c c t g t t c a a g a a a g a c a t t a t c g a g a c t a t t c t g c c t g a g t t c t t g c t c c c a a t g t t c a g g g a c a a g t t c t t t c t g t a a t a g c t c t g a t a a g a c g g a c t c a a g

1620 (in frame with SV40 NLS) LDDKDEIALVNSFNGFTTAF LDDKDEIALVNSFNGFTTAF c t g g a c g a t a a a g a t g a g a t c g c c c t c g t c a a t t c c t t c a a t g g g t t t a c c a c a g c c t t t g a c c t g c t a t t t c t a c t c t a g c g g g a g c a g t t a a g g a a g t t a c c c a a a t g g t g t c g g a a a

1680 (in frame with SV40 NLS) TGFFDNRENMFSEEAKSTSI TGFFDNRENMFSEEAKSTSI a c c g g c t t c t t c g a c a a t a g a g a g a a t a t g t t c t c t g a a g a g g c c a a a t c c a c t a g c a t c t g g c c g a a g a a g c t g t t a t c t c t c t t a t a c a a g a g a c t t c t c c g g t t t a g g t g a t c g t a g

1740 (in frame with SV40 NLS) AFRCINENLTRYISNMDIFE AFRCINENLTRYISNMDIFE g c c t t t c g c t g c a t a a a c g a g a a c c t g a c t a g g t a c a t c a g c a a t a t g g a c a t c t t t g a g c g g a a a g c g a c g t a t t t g c t c t t g g a c t g a t c c a t g t a g t c g t t a t a c c t g t a g a a a c t c

1800 (in frame with SV40 NLS) KVDAIFDKHEVQEIKEKILN KVDAIFDKHEVQEIKEKILN a a a g t c g a t g c c a t a t t c g a c a a a c a t g a g g t g c a g g a g a t t a a g g a g a a g a t c c t g a a c t t t c a g c t a c g g t a t a a g c t g t t t g t a c t c c a c g t c c t c t a a t t c c t c t t c t a g g a c t t g

1860 (in frame with SV40 NLS) SDYDVEDFFEGEFFNFVLTQ SDYDVEDFFEGEFFNFVLTQ t c a g a t t a c g a t g t c g a a g a t t t c t t c g a g g g a g a g t t c t t c a a c t t c g t g c t c a c a c a a a g t c t a a t g c t a c a g c t t c t a a a g a a g c t c c c t c t c a a g a a g t t g a a g c a c g a g t g t g t t

1920 (in frame with SV40 NLS) EGIDVYNAIIGGFVTESGEK EGIDVYNAIIGGFVTESGEK g a g g g c a t t g a t g t g t a c a a t g c a a t c a t t g g a g g g t t c g t g a c a g a g a g t g g t g a g a a g c t c c c g t a a c t a c a c a t g t t a c g t t a g t a a c c t c c c a a g c a c t g t c t c t c a c c a c t c t t c

1980 (in frame with SV40 NLS) IKGLNEYINLYNQKTKQKLP IKGLNEYINLYNQKTKQKLP a t a a a g g g c c t g a a c g a g t a t a t c a a c c t c t a c a a c c a g a a a a c c a a g c a g a a a c t g c c t t a t t t c c c g g a c t t g c t c a t a t a g t t g g a g a t g t t g g t c t t t t g g t t c g t c t t t g a c g g a

2040 (in frame with SV40 NLS) KFKPLYKQVLSDRESLSFYG KFKPLYKQVLSDRESLSFYG a a g t t c a a g c c a c t g t a c a a a c a a g t g c t c t c a g a t a g g g a a a g c c t g a g c t t c t a c g g t t t c a a g t t c g g t g a c a t g t t t g t t c a c g a g a g t c t a t c c c t t t c g g a c t c g a a g a t g c c a

BstZ17I 2100 (in frame with SV40 NLS) EGYTSDEEVLEVFRNTLNKN EGYTSDEEVLEVFRNTLNKN g a a g g g t a t a c a t c a g a t g a a g a a g t g c t c g a a g t g t t c c g c a a c a c c c t c a a t a a g a a c c t t c c c a t a t g t a g t c t a c t t c t t c a c g a g c t t c a c a a g g c g t t g t g g g a g t t a t t c t t g

2160 (in frame with SV40 NLS) SEIFSSIKKLEKLFKNFDEY SEIFSSIKKLEKLFKNFDEY a g t g a a a t c t t c t c t t c a a t c a a g a a g c t g g a g a a a c t g t t c a a g a a t t t c g a t g a g t a c t c a c t t t a g a a g a g a a g t t a g t t c t t c g a c c t c t t t g a c a a g t t c t t a a a g c t a c t c a t g

2220 (in frame with SV40 NLS) SSAGIFVKNGPAISTISKDI SSAGIFVKNGPAISTISKDI t c c t c t g c c g g a a t c t t t g t g a a g a a t g g c c c t g c a a t a t c c a c t a t t a g c a a a g a c a t c a g g a g a c g g c c t t a g a a a c a c t t c t t a c c g g g a c g t t a t a g g t g a t a a t c g t t t c t g t a g

2280 (in frame with SV40 NLS) FGEWNVIRDKWNAEYDDIHL FGEWNVIRDKWNAEYDDIHL t t t g g c g a g t g g a a c g t t a t c a g g g a t a a g t g g a a t g c c g a g t a c g a t g a t a t t c a t c t c a a a c c g c t c a c c t t g c a a t a g t c c c t a t t c a c c t t a c g g c t c a t g c t a c t a t a a g t a g a g

2340 (in frame with SV40 NLS) KKKAVVTEKYEDDRRKSFKK KKKAVVTEKYEDDRRKSFKK a a g a a g a a a g c c g t g g t t a c a g a g a a a t a c g a g g a t g a t a g a c g c a a g a g c t t t a a g a a g t t c t t c t t t c g g c a c c a a t g t c t c t t t a t g c t c c t a c t a t c t g c g t t c t c g a a a t t c t t c

PstI 2400 (in frame with SV40 NLS) IGSFSLEQLQEYADADLSVV IGSFSLEQLQEYADADLSVV a t t g g t a g c t t c t c t c t c g a a c a g c t g c a g g a g t a c g c c g a c g c t g a c c t g t c a g t c g t g t a a c c a t c g a a g a g a g a g c t t g t c g a c g t c c t c a t g c g g c t g c g a c t g g a c a g t c a g c a c

2460 (in frame with SV40 NLS) EKLKEIIIQKVDEIYKVYGS EKLKEIIIQKVDEIYKVYGS g a g a a a c t c a a g g a g a t c a t a a t c c a g a a g g t g g a t g a a a t c t a c a a a g t g t a t g g a a g c c t c t t t g a g t t c c t c t a g t a t t a g g t c t t c c a c c t a c t t t a g a t g t t t c a c a t a c c t t c g

2520 (in frame with SV40 NLS) SEKLFDADFVLEKSLKKNDA SEKLFDADFVLEKSLKKNDA t c t g a g a a a c t c t t c g a t g c a g a c t t t g t t c t g g a g a a g a g t c t g a a g a a g a a c g a c g c a a g a c t c t t t g a g a a g c t a c g t c t g a a a c a a g a c c t c t t c t c a g a c t t c t t c t t g c t g c g t

2580 (in frame with SV40 NLS) VVAIMKDLLDSVKSFENYIK VVAIMKDLLDSVKSFENYIK g t g g t t g c t a t c a t g a a g g a c c t g c t g g a t t c t g t t a a g t c t t t c g a g a a t t a c a t t a a g c a c c a a c g a t a g t a c t t c c t g g a c g a c c t a a g a c a a t t c a g a a a g c t c t t a a t g t a a t t c

2640 (in frame with SV40 NLS) AFFGEGKETNRDESFYGDFV AFFGEGKETNRDESFYGDFV g c a t t c t t t g g t g a a g g g a a g g a g a c a a a t a g g g a c g a g a g c t t c t a t g g c g a c t t t g t t c g t a a g a a a c c a c t t c c c t t c c t c t g t t t a t c c c t g c t c t c g a a g a t a c c g c t g a a a c a a

2700 (in frame with SV40 NLS) LAYDILLKVDHIYDAIRNYV LAYDILLKVDHIYDAIRNYV c t g g c c t a c g a c a t c c t c c t c a a g g t t g a c c a c a t c t a t g a c g c t a t a c g g a a t t a c g t t g a c c g g a t g c t g t a g g a g g a g t t c c a a c t g g t g t a g a t a c t g c g a t a t g c c t t a a t g c a a

2760 (in frame with SV40 NLS) TQKPYSKDKFKLYFQNPQFM TQKPYSKDKFKLYFQNPQFM a c c c a g a a g c c c t a t a g c a a a g a c a a g t t c a a g c t g t a t t t c c a g a a t c c a c a g t t t a t g t g g g t c t t c g g g a t a t c g t t t c t g t t c a a g t t c g a c a t a a a g g t c t t a g g t g t c a a a t a c

2820 (in frame with SV40 NLS) GGWDKDKETDYRATILRYGS GGWDKDKETDYRATILRYGS g g t g g g t g g g a t a a a g a c a a a g a a a c a g a t t a c a g g g c c a c t a t c c t g c g g t a c g g c a g c c c a c c c a c c c t a t t t c t g t t t c t t t g t c t a a t g t c c c g g t g a t a g g a c g c c a t g c c g t c g

2880 (in frame with SV40 NLS) KYYLAIMDKKYAKCLQKIDK KYYLAIMDKKYAKCLQKIDK a a a t a c t a t c t g g c t a t c a t g g a t a a g a a g t a c g c c a a a t g c c t c c a g a a g a t c g a c a a g t t t a t g a t a g a c c g a t a g t a c c t a t t c t t c a t g c g g t t t a c g g a g g t c t t c t a g c t g t t c

2940 (in frame with SV40 NLS) DDVNGNYEKINYKLLPGPNK DDVNGNYEKINYKLLPGPNK g a c g a c g t g a a c g g t a a c t a c g a g a a g a t c a a t t a c a a g c t c c t g c c a g g a c c t a a c a a g c t g c t g c a c t t g c c a t t g a t g c t c t t c t a g t t a a t g t t c g a g g a c g g t c c t g g a t t g t t c

3000 (in frame with SV40 NLS) MLPKVFFSKKWMAYYNPSED MLPKVFFSKKWMAYYNPSED a t g c t g c c c a a g g t g t t c t t c t c c a a g a a a t g g a t g g c c t a c t a t a a c c c a a g c g a g g a c t a c g a c g g g t t c c a c a a g a a g a g g t t c t t t a c c t a c c g g a t g a t a t t g g g t t c g c t c c t g

3060 (in frame with SV40 NLS) IQKIYKNGTFKKGDMFNLND IQKIYKNGTFKKGDMFNLND a t t c a g a a g a t a t a c a a g a a t g g g a c a t t c a a g a a g g g c g a t a t g t t c a a c c t c a a c g a c t a a g t c t t c t a t a t g t t c t t a c c c t g t a a g t t c t t c c c g c t a t a c a a g t t g g a g t t g c t g

3120 (in frame with SV40 NLS) CHKLIDFFKDSISRYPKWSN CHKLIDFFKDSISRYPKWSN t g c c a c a a g c t g a t t g a t t t c t t c a a g g a t a g c a t t t c t c g c t a t c c c a a g t g g t c t a a t a c g g t g t t c g a c t a a c t a a a g a a g t t c c t a t c g t a a a g a g c g a t a g g g t t c a c c a g a t t a

NsiI 3180 (in frame with SV40 NLS) AYDFNFSETEKYKDIAGFYR AYDFNFSETEKYKDIAGFYR g c a t a c g a t t t c a a c t t c a g c g a g a c t g a g a a g t a c a a a g a c a t c g c t g g c t t c t a c c g g c g t a t g c t a a a g t t g a a g t c g c t c t g a c t c t t c a t g t t t c t g t a g c g a c c g a a g a t g g c c

3240 (in frame with SV40 NLS) EVEEQGYKVSFESASKKEVD EVEEQGYKVSFESASKKEVD g a g g t g g a a g a g c a a g g c t a t a a g g t g t c a t t c g a a t c c g c t t c t a a g a a g g a a g t g g a t c t c c a c c t t c t c g t t c c g a t a t t c c a c a g t a a g c t t a g g c g a a g a t t c t t c c t t c a c c t a

3300 (in frame with SV40 NLS) KLVEEGKLYMFQIYNKDFSD KLVEEGKLYMFQIYNKDFSD a a g c t c g t g g a a g a g g g t a a g c t g t a c a t g t t c c a g a t a t a c a a c a a a g a c t t c a g c g a t t t c g a g c a c c t t c t c c c a t t c g a c a t g t a c a a g g t c t a t a t g t t g t t t c t g a a g t c g c t a

3360 (in frame with SV40 NLS) KSHGTPNLHTMYFKLLFDEN KSHGTPNLHTMYFKLLFDEN a a g a g c c a c g g c a c t c c a a a c c t c c a t a c t a t g t a t t t c a a g c t g c t g t t t g a c g a g a a c t t c t c g g t g c c g t g a g g t t t g g a g g t a t g a t a c a t a a a g t t c g a c g a c a a a c t g c t c t t g

3420 (in frame with SV40 NLS) NHGQIRLSGGAELFMRRASL NHGQIRLSGGAELFMRRASL a a c c a c g g a c a g a t t a g g c t g t c a g g a g g c g c a g a a c t c t t c a t g c g c a g a g c t t c a c t g t t g g t g c c t g t c t a a t c c g a c a g t c c t c c g c g t c t t g a g a a g t a c g c g t c t c g a a g t g a c

3480 (in frame with SV40 NLS) KKEELVVHPANSPIANKNPD KKEELVVHPANSPIANKNPD a a g a a g g a g g a a c t c g t t g t c c a c c c a g c c a a t a g c c c t a t a g c c a a t a a g a a t c c a g a c t t c t t c c t c c t t g a g c a a c a g g t g g g t c g g t t a t c g g g a t a t c g g t t a t t c t t a g g t c t g

3540 (in frame with SV40 NLS) NPKKTTTLSYDVYKDKRFSE NPKKTTTLSYDVYKDKRFSE a a t c c t a a g a a a a c c a c t a c t c t g t c t t a c g a t g t g t a t a a g g a t a a g a g a t t c t c t g a a t t a g g a t t c t t t t g g t g a t g a g a c a g a a t g c t a c a c a t a t t c c t a t t c t c t a a g a g a c t t

3600 (in frame with SV40 NLS) DQYELHIPIAINKCPKNIFK DQYELHIPIAINKCPKNIFK g a t c a g t a c g a a c t g c a c a t a c c c a t t g c c a t t a a c a a g t g c c c t a a g a a c a t c t t c a a g c t a g t c a t g c t t g a c g t g t a t g g g t a a c g g t a a t t g t t c a c g g g a t t c t t g t a g a a g t t c

3660 (in frame with SV40 NLS) INTEVRVLLKHDDNPYVIGI INTEVRVLLKHDDNPYVIGI a t t a a c a c a g a g g t t a g a g t g c t c c t g a a a c a c g a c g a t a a c c c t t a t g t t a t a g g c a t t t a a t t g t g t c t c c a a t c t c a c g a g g a c t t t g t g c t g c t a t t g g g a a t a c a a t a t c c g t a a

3720 (in frame with SV40 NLS) ARGERNLLYIVVVDGKGNIV ARGERNLLYIVVVDGKGNIV g c t c g c g g a g a g a g a a a c c t g c t g t a c a t c g t c g t g g t g g a c g g c a a a g g c a a c a t c g t g c g a g c g c c t c t c t c t t t g g a c g a c a t g t a g c a g c a c c a c c t g c c g t t t c c g t t g t a g c a c

3780 (in frame with SV40 NLS) EQYSLNEIINNFNGIRIKTD EQYSLNEIINNFNGIRIKTD g a a c a g t a c a g t c t c a a t g a a a t c a t t a a c a a t t t c a a c g g a a t c c g c a t t a a g a c c g a c c t t g t c a t g t c a g a g t t a c t t t a g t a a t t g t t a a a g t t g c c t t a g g c g t a a t t c t g g c t g

3840 (in frame with SV40 NLS) YHSLLDKKEKERFEARQNWT YHSLLDKKEKERFEARQNWT t a c c a t t c t c t c c t c g a c a a g a a g g a g a a a g a a a g g t t c g a a g c a a g a c a g a a t t g g a c a a t g g t a a g a g a g g a g c t g t t c t t c c t c t t t c t t t c c a a g c t t c g t t c t g t c t t a a c c t g t

3900 (in frame with SV40 NLS) SIENIKELKAGYISQVVHKI SIENIKELKAGYISQVVHKI a g t a t a g a g a a t a t c a a a g a a c t g a a g g c t g g g t a c a t c t c t c a g g t t g t g c a c a a g a t a t c a t a t c t c t t a t a g t t t c t t g a c t t c c g a c c c a t g t a g a g a g t c c a a c a c g t g t t c t a t

3960 (in frame with SV40 NLS) CELVEKYDAVIALADLNSGF CELVEKYDAVIALADLNSGF t g t g a g c t g g t g g a g a a g t a c g a c g c t g t t a t c g c c c t c g c g g a c c t g a a t a g c g g c t t c a c a c t c g a c c a c c t c t t c a t g c t g c g a c a a t a g c g g g a g c g c c t g g a c t t a t c g c c g a a g

PaqCI 4020 (in frame with SV40 NLS) KNSRVKVEKQVYQKFEKMLI KNSRVKVEKQVYQKFEKMLI a a g a a c t c c a g g g t g a a g g t g g a g a a g c a g g t g t a t c a g a a g t t c g a g a a g a t g c t g a t c t t c t t g a g g t c c c a c t t c c a c c t c t t c g t c c a c a t a g t c t t c a a g c t c t t c t a c g a c t a g

4080 (in frame with SV40 NLS) DKLNYMVDKKSNPCATGGAL DKLNYMVDKKSNPCATGGAL g a c a a g c t c a a c t a t a t g g t g g a c a a g a a a t c c a a t c c t t g c g c t a c t g g t g g a g c c c t g c t g t t c g a g t t g a t a t a c c a c c t g t t c t t t a g g t t a g g a a c g c g a t g a c c a c c t c g g g a c

4140 (in frame with SV40 NLS) KGYQITNKFESFKSMSTQNG KGYQITNKFESFKSMSTQNG a a g g g c t a t c a a a t c a c c a a t a a g t t c g a a t c t t t c a a g t c t a t g a g c a c c c a g a a t g g c t t c c c g a t a g t t t a g t g g t t a t t c a a g c t t a g a a a g t t c a g a t a c t c g t g g g t c t t a c c g

4200 (in frame with SV40 NLS) FIFYIPAWLTSKIDPSTGFV FIFYIPAWLTSKIDPSTGFV t t c a t c t t c t a c a t a c c c g c a t g g c t g a c a t c c a a g a t t g a t c c c t c t a c c g g a t t t g t t a a g t a g a a g a t g t a t g g g c g t a c c g a c t g t a g g t t c t a a c t a g g g a g a t g g c c t a a a c a a

4260 (in frame with SV40 NLS) NLLKTKYTSIADSKKFISSF NLLKTKYTSIADSKKFISSF a a t c t g c t c a a g a c t a a g t a c a c c t c t a t t g c t g a c t c a a a g a a g t t c a t a t c a t c a t t t t t a g a c g a g t t c t g a t t c a t g t g g a g a t a a c g a c t g a g t t t c t t c a a g t a t a g t a g t a a a

4320 (in frame with SV40 NLS) DRIMYVPEEDLFEFALDYKN DRIMYVPEEDLFEFALDYKN g a c c g c a t c a t g t a c g t g c c a g a a g a g g a c c t g t t c g a g t t t g c c c t g g a t t a c a a g a a t c t g g c g t a g t a c a t g c a c g g t c t t c t c c t g g a c a a g c t c a a a c g g g a c c t a a t g t t c t t a

4380 (in frame with SV40 NLS) FSRTDADYIKKWKLYSYGNR FSRTDADYIKKWKLYSYGNR t t c t c t c g g a c t g a c g c c g a c t a c a t c a a g a a g t g g a a g c t c t a c t c t t a t g g t a a t c g g a a g a g a g c c t g a c t g c g g c t g a t g t a g t t c t t c a c c t t c g a g a t g a g a a t a c c a t t a g c c

4440 (in frame with SV40 NLS) IRIFRNPKKNNVFDWEEVCL IRIFRNPKKNNVFDWEEVCL a t t c g c a t a t t c c g c a a t c c c a a g a a g a a t a a c g t g t t c g a t t g g g a g g a a g t t t g c c t c t a a g c g t a t a a g g c g t t a g g g t t c t t c t t a t t g c a c a a g c t a a c c c t c c t t c a a a c g g a g

AfeI 4500 (in frame with SV40 NLS) TSAYKELFNKYGINYQQGDI TSAYKELFNKYGINYQQGDI a c c a g c g c t t a c a a g g a g c t g t t c a a t a a g t a t g g g a t t a a c t a c c a g c a g g g c g a c a t a t g g t c g c g a a t g t t c c t c g a c a a g t t a t t c a t a c c c t a a t t g a t g g t c g t c c c g c t g t a t

4560 (in frame with SV40 NLS) RALLCEQSDKAFYSSFMALM RALLCEQSDKAFYSSFMALM a g a g c c c t g c t g t g c g a a c a a t c t g a t a a g g c a t t c t a t t c c t c t t t c a t g g c a c t g a t g t c t c g g g a c g a c a c g c t t g t t a g a c t a t t c c g t a a g a t a a g g a g a a a g t a c c g t g a c t a c

4620 (in frame with SV40 NLS) SLMLQMRNSITGRTDVAFLI SLMLQMRNSITGRTDVAFLI t c a c t g a t g c t g c a a a t g c g c a a t t c c a t c a c c g g a a g a a c a g a c g t g g c c t t t c t g a t c a g t g a c t a c g a c g t t t a c g c g t t a a g g t a g t g g c c t t c t t g t c t g c a c c g g a a a g a c t a g

4680 (in frame with SV40 NLS) SPVKNSDGIFYDSRNYEAQE SPVKNSDGIFYDSRNYEAQE t c t c c t g t c a a g a a c t c a g a t g g c a t c t t c t a c g a t t c c c g c a a c t a t g a a g c a c a g g a g a g a g g a c a g t t c t t g a g t c t a c c g t a g a a g a t g c t a a g g g c g t t g a t a c t t c g t g t c c t c

4740 (in frame with SV40 NLS) NAILPKNADANGAYNIARKV NAILPKNADANGAYNIARKV a a t g c t a t c c t g c c t a a g a a t g c c g a t g c a a a t g g a g c c t a t a a c a t c g c c a g a a a g g t c t t a c g a t a g g a c g g a t t c t t a c g g c t a c g t t t a c c t c g g a t a t t g t a g c g g t c t t t c c a g

4800 (in frame with SV40 NLS) LWAIGQFKKAEDEKLDKVKI LWAIGQFKKAEDEKLDKVKI c t c t g g g c c a t a g g a c a a t t c a a g a a a g c t g a a g a t g a g a a g c t g g a c a a g g t g a a g a t c g a g a c c c g g t a t c c t g t t a a g t t c t t t c g a c t t c t a c t c t t c g a c c t g t t c c a c t t c t a g

4860 (in frame with SV40 NLS) AISNKEWLEYAQTSVKHGS P SV40 NLS AISNKEWLEYAQTSVKHGSP g c c a t t t c a a a c a a a g a g t g g c t c g a a t a t g c t c a g a c c t c a g t g a a g c a t g g a t c a c c c c g g t a a a g t t t g t t t c t c a c c g a g c t t a t a c g a g t c t g g a g t c a c t t c g t a c c t a g t g g g

4920 130 KKKRKV SGGS 5 10 TNLSDIIEKE SV40 NLS UGI KKKRKVSGGSTNLSDIIEKE a a g a a g a a a c g g a a a g t g t c t g g t g g t t c t a c t a a t c t g t c a g a t a t t a t t g a a a a g g a g t t c t t c t t t g c c t t t c a c a g a c c a c c a a g a t g a t t a g a c a g t c t a t a a t a a c t t t t c c t c

4980 15 20 25 30 TGKQLVIQESILMLPEEVEE UGI TGKQLVIQESILMLPEEVEE a c c g g t a a g c a a c t g g t t a t c c a g g a a t c c a t c c t c a t g c t c c c a g a g g a g g t g g a a g a a t g g c c a t t c g t t g a c c a a t a g g t c c t t a g g t a g g a g t a c g a g g g t c t c c t c c a c c t t c t t

5040 35 40 45 50 VIGNKPESDILVHTAYDEST UGI VIGNKPESDILVHTAYDEST g t c a t t g g g a a c a a g c c g g a a a g c g a t a t a c t c g t g c a c a c c g c c t a c g a c g a g a g c a c c c a g t a a c c c t t g t t c g g c c t t t c g c t a t a t g a g c a c g t g t g g c g g a t g c t g c t c t c g t g g

5100 55 60 65 70 DENVMLLTSDAPEYKPWALV UGI DENVMLLTSDAPEYKPWALV g a c g a g a a t g t c a t g c t t c t g a c t a g c g a c g c c c c t g a a t a c a a g c c t t g g g c t c t g g t c c t g c t c t t a c a g t a c g a a g a c t g a t c g c t g c g g g g a c t t a t g t t c g g a a c c c g a g a c c a g

5160 75 80 IQDSNGENKIKML (in frame with UGI) SGGS PKK UGI SV40 NLS IQDSNGENKIKMLSGGSPKK a t a c a g g a t a g c a a c g g t g a g a a c a a g a t t a a g a t g c t c t c t g g t g g t t c t c c c a a g a a g t a t g t c c t a t c g t t g c c a c t c t t g t t c t a a t t c t a c g a g a g a c c a c c a a g a g g g t t c t t c

BclI*PmeI 5220 130 KRKV * 1 5 HHHHHH * SV40 NLS 6xHis KRKV* a a g a g g a a a g t c t a a c c g g t c a t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g c t g a t c a g t t c t c c t t t c a g a t t g g c c a g t a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c g a c t a g t c

5280 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a

5340 bGH poly(A) signal t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g

5400 bGH poly(A) signal a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c

SphI 5460 bGH poly(A) signal a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c

5520 c g g a a a g a a c c a g c t g g g g c t c g a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g c c t t t c t t g g t c g a c c c c g a g c t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a

5580 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c

5640 -35 lac promoter CAP binding site c c g g a a g c a t a a a g t g t a a a g c c t a g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g g g c c t t c g t a t t t c a c a t t t c g g a t c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c

5700 c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t

5760 G A G C G A G T L4440 t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t

5820 G A C T G A G C G A L4440 c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c

5880 t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g

5940 ori a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g

6000 ori c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g

6060 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g

6120 ori t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t

6180 ori g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g

6240 ori a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t

6300 ori a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c

6360 ori c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t

6420 ori g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c

6480 ori g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g

6540 ori a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a

6600 c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t

6660 g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a

6720 285 280 275 *WHKILSAGIEAI AmpR *WHKILSAGIEAI a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t

AhdI 6780 270 265 260 255 QRNREDMTAQSGTTYIVVIR AmpR QRNREDMTAQSGTTYIVVIR t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g

6840 250 245 240 235 SPKGDPGLAAIIGRSGREGA AmpR SPKGDPGLAAIIGRSGREGA g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c

6900 230 225 220 215 GSKDAIFWGAPLASRLLPGA AmpR GSKDAIFWGAPLASRLLPGA c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c

6960 210 205 200 195 VKDAEMWDILQQRSALTLLE AmpR VKDAEMWDILQQRSALTLLE c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a

7020 190 185 180 175 GTLLKRLTTAMAVPMTTDRE AmpR GTLLKRLTTAMAVPMTTDRE c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a

7080 170 165 160 155 DNPIAENLEPEWRDLRTVHD AmpR DNPIAENLEPEWRDLRTVHD c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a

PvuI 7140 150 145 140 135 GMNHLFATLEKPGGITTLLL AmpR GMNHLFATLEKPGGITTLLL c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t

7200 130 125 120 115 NAATNDSMTIAASCLERVTM AmpR NAATNDSMTIAASCLERVTM a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t

7260 110 105 100 95 GDTLHKETVPSYEVLDNQSY AmpR GDTLHKETVPSYEVLDNQSY t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a

7320 90 85 80 75 HIRRGLQEQGADIRSLVAGC AmpR A T A A T A C C G C G C C A C Amp-R HIRRGLQEQGADIRSLVAGC a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g

7380 70 65 60 55 LLVKFTSMMPFREEPRFSEL AmpR A T A G C Amp-R LLVKFTSMMPFREEPRFSEL a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t

7440 50 45 40 35 IKGSNLDLEIYGVRAGLQDE AmpR IKGSNLDLEIYGVRAGLQDE g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a

7500 30 25 ADKVKVLTEPH 20 15 signal sequence AFVPLCFAA AmpR ADKVKVLTEPHAFVPLCFAA c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c

SspI 7560 10 5 1 signal sequence FFPILAVRFHQISM AmpR AmpR promoter FFPILAVRFHQISM c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a

7620 AmpR promoter a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a

SgrDI 7680 AmpR promoter a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c

BspDI*ClaI* 7740 A T G T T A G A C G A G A C pRS-marker a c g g a t c g g g a g a t c g a t c t c c c g a t c c c c t a g g g t c g a c t c t c a g t a c a a t c t g c t c t g t g c c t a g c c c t c t a g c t a g a g g g c t a g g g g a t c c c a g c t g a g a g t c a t g t t a g a c g a g a c

7800 T A C G G C pRS-marker a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a

MfeI 7860 g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g

MluINruI 7920 CMV enhancer t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g

SpeI 7980 CMV enhancer a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a

8040 CMV enhancer a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c

8100 CMV enhancer a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a

8160 CMV enhancer t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c

3ʹ 5ʹ 8166 CMV enhancer t g t a t c a c a t a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.