Addgene p6268 pDEST-59-His-Trx-HPV16E6 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: p6268 pDEST-59-His-Trx-HPV16E6 Sequencing Result

Map View

600 400 200 End (792) DraI (707) BsaBI (677) NsiI (661) BmgBI (625) AlwNI (618) MslI (549) PpuMI - EcoO109I (516) BsrDI (512) BsiEI (473) NotI - EaeI - EagI - SacII (470) MspA1I (469) BsrGI (449) PmlI - BsaAI (430) SacI - BanII (420) Eco53kI (418) AvaI - BsoBI (413) XmnI (391) BaeGI - Bme1580I (369) ApaLI (365) BciVI (322) BtsI - BtsαI (284) ClaI * - BspDI * (269) RsrII (187) BmtI (92) NheI (88) TsoI (76) StyI - NcoI (47) ApoI - EcoRI (4) Start (0) 6xHis TrxA thrombin site attB1 p6268 pDEST-59-His-Trx-HPV16E6 792 bp

Sequence View

NcoIStyIStart (0) EcoRIApoI 5ʹ 3ʹ 60 VEFPLNILFTLRRRYTMGSS g t n g a a t t c c c t c t g a a t a t t t t g t t t a c t t t a a g a a g g a g a t a t a c c a t g g g c a g c a g c c a n c t t a a g g g a g a c t t a t a a a a c a a a t g a a a t t c t t c c t c t a t a t g g t a c c c g t c g t c g

BmtINheITsoI 120 1 5 HHHHHH SH 1 5 10 MASDKIIHLTDD 6xHis TrxA HHHHHHSHMASDKIIHLTDD c a t c a t c a t c a t c a t c a c a g c c a t a t g g c t a g c g a t a a a a t t a t t c a c c t g a c t g a c g a c g t a g t a g t a g t a g t a g t g t c g g t a t a c c g a t c g c t a t t t t a a t a a g t g g a c t g a c t g c t g

180 15 20 25 30 SFDTDVLKADGAILVDFWAE TrxA SFDTDVLKADGAILVDFWAE a g t t t t g a c a c g g a t g t a c t c a a a g c g g a c g g g g c g a t c c t c g t c g a t t t c t g g g c a g a g t c a a a a c t g t g c c t a c a t g a g t t t c g c c t g c c c c g c t a g g a g c a g c t a a a g a c c c g t c t c

RsrII 240 35 40 45 50 WCGPCKMIAPILDEIADEYQ TrxA WCGPCKMIAPILDEIADEYQ t g g t g c g g t c c g t g c a a a a t g a t c g c c c c g a t t c t g g a t g a a a t c g c t g a c g a a t a t c a g a c c a c g c c a g g c a c g t t t t a c t a g c g g g g c t a a g a c c t a c t t t a g c g a c t g c t t a t a g t c

BtsαIBtsIBspDI*ClaI* 300 55 60 65 70 GKLTVAKLNIDQNPGTAPKY TrxA GKLTVAKLNIDQNPGTAPKY g g c a a a c t g a c c g t t g c a a a a c t g a a c a t c g a t c a a a a c c c t g g c a c t g c g c c g a a a t a t c c g t t t g a c t g g c a a c g t t t t g a c t t g t a g c t a g t t t t g g g a c c g t g a c g c g g c t t t a t a

BciVI 360 75 80 85 90 GIRGIPTLLLFKNGEVAATK TrxA GIRGIPTLLLFKNGEVAATK g g c a t c c g t g g t a t c c c g a c t c t g c t g c t g t t c a a a a a c g g t g a a g t g g c g g c a a c c a a a c c g t a g g c a c c a t a g g g c t g a g a c g a c g a c a a g t t t t t g c c a c t t c a c c g c c g t t g g t t t

BsoBIAvaIEco53kIBanIISacIXmnIApaLIBme1580IBaeGI 420 95 100 105 110 VGALSKGQLKEFLDANLA RA TrxA VGALSKGQLKEFLDANLARA g t g g g t g c a c t g t c t a a a g g t c a g t t g a a a g a g t t c c t c g a c g c t a a c c t g g c c c g a g c t c a c c c a c g t g a c a g a t t t c c a g t c a a c t t t c t c a a g g a g c t g c g a t t g g a c c g g g c t c g a

MspA1ISacIIEagIEaeINotIBsiEIBsrGIBsaAIPmlI 480 1 5 LVPRGS (in frame with thrombin site) VTSLYKKAGSAAAP thrombin site attB1 LVPRGSVTSLYKKAGSAAAP c t t g t t c c a c g t g g t t c t g t c a c a a g t t t g t a c a a a a a a g c a g g c t c c g c g g c c g c c c c c g a a c a a g g t g c a c c a a g a c a g t g t t c a a a c a t g t t t t t t c g t c c g a g g c g c c g g c g g g g g

BsrDIEcoO109IPpuMI 540 (in frame with thrombin site) FTMHQKRTAMFQDPQERPRK FTMHQKRTAMFQDPQERPRK t t c a c c a t g c a c c a a a a g a g a a c t g c a a t g t t t c a g g a c c c a c a g g a g c g a c c c a g a a a g a a g t g g t a c g t g g t t t t c t c t t g a c g t t a c a a a g t c c t g g g t g t c c t c g c t g g g t c t t t c

MslI 600 (in frame with thrombin site) LPQLCTELQTTIHDIILECV LPQLCTELQTTIHDIILECV t t a c c a c a g t t a t g c a c a g a g c t g c a a a c a a c t a t a c a t g a t a t a a t a t t a g a a t g t g t g a a t g g t g t c a a t a c g t g t c t c g a c g t t t g t t g a t a t g t a c t a t a t t a t a a t c t t a c a c a c

BmgBIAlwNI 660 (in frame with thrombin site) YCKQQLLRREVYDFAFRDLC YCKQQLLRREVYDFAFRDLC t a c t g c a a g c a a c a g t t a c t g c g a c g t g a g g t a t a t g a c t t t g c t t t t c g g g a t t t a t g c a t g a c g t t c g t t g t c a a t g a c g c t g c a c t c c a t a t a c t g a a a c g a a a a g c c c t a a a t a c g

DraIBsaBINsiI 720 (in frame with thrombin site) IVYRDGNPYAVCDKCLKFYS IVYRDGNPYAVCDKCLKFYS a t a g t a t a t a g a g a t g g g a a t c c a t a t g c t g t a t g t g a t a a a t g t t t a a a g t t t t a t t c t t a t c a t a t a t c t c t a c c c t t a g g t a t a c g a c a t a c a c t a t t t a c a a a t t t c a a a a t a a g a

780 (in frame with thrombin site) KISEYRXIVIVCMDT* KISEYRXIVIVCMDT* a a a a t t a g t g a g t a t a g a c n t a t t g t t a t a g t t t g t a t g g a c a c n t a g a c a g c a t a c a c a t t t t a a t c a c t c a t a t c t g n a t a a c a a t a t c a a a c a t a c c t g t g n a t c t g t c g t a t g t g t

End (792) 3ʹ 5ʹ 792 c c g t t t g g c n t t g g c a a a c c g n a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.