Addgene pEGFP - FLAG-APEX2- hs Lamin B1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pEGFP - FLAG-APEX2- hs Lamin B1 Sequencing Result


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NdeI (6369) CMV enhancer AseI (6142) AflIII - PciI (6077) pBR322ori-F (5922 .. 5941) ApaLI (5763) TK-pA-R (5013 .. 5032) Neo-F (4695 .. 4714) RsrII (4675) BsrDI (4392) PflFI - Tth111I (4277) MscI (4241) Neo-R (4085 .. 4104) StuI (3980) SfiI (3934) pBABE 3' (3734 .. 3754) F1ori-R (3472 .. 3491) F1ori-F (3260 .. 3281) CMV-F (144 .. 164) NheI (216) BmtI (220) AfeI (221) AgeI (225) FspAI (357) PspOMI (588) ApaI (592) EcoRV (726) KflI - PpuMI (790) AhdI (919) BspEI (1042) BglII (1051) PaeR7I - XhoI (1055) SacII (1132) BlpI (1168) BsrGI (1238) BsmBI - Esp3I (1258) PaqCI (1281) HindIII (1504) EcoRI (1530) BsgI (1541) EcoNI (1708) BbvCI (2412) BamHI (2866) MfeI (2981) HpaI (2994) SV40pA-R (3032 .. 3051) BtsI - BtsαI (3070) EBV-rev (3086 .. 3105) AflII (3113) pEGFP - FLAG-APEX2- hs Lamin B1 6510 bp

Sequence View

5ʹ 3ʹ 60 CMV promoter t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t

120 CMV promoter c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c

180 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t

AgeINheIBmtIAfeI 240 CMV promoter Kozak sequence M t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g t c g c c a c c a t g a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c a g c g g t g g t a c

300 1 5 DYKDDDDK (in frame with FLAG) GKSYPTVSADYQ FLAG Kozak sequence DYKDDDDKGKSYPTVSADYQ *VWCHGCVVL g a c t a c a a g g a t g a c g a c g a t a a g g g g a a a t c a t a c c c a a c a g t g t c c g c a g a c t a c c a g c t g a t g t t c c t a c t g c t g c t a t t c c c c t t t a g t a t g g g t t g t c a c a g g c g t c t g a t g g t c

FspAI 360 (in frame with FLAG) DAVEKAKKKLRGFIAEKRCA DAVEKAKKKLRGFIAEKRCA IGHLFGLLLQSPKDCFLPAC g a t g c c g t g g a g a a a g c c a a g a a g a a g c t g a g a g g g t t t a t c g c a g a a a a g a g g t g c g c a c t a c g g c a c c t c t t t c g g t t c t t c t t c g a c t c t c c c a a a t a g c g t c t t t t c t c c a c g c g t

420 (in frame with FLAG) PLMLRLAFHSAGTFDKGTKT PLMLRLAFHSAGTFDKGTKT RQHQSQSEVACAGKVLSGFC c c t c t g a t g c t g a g a c t g g c t t t c c a c a g c g c a g g c a c c t t t g a c a a g g g a a c c a a a a c a g g a g a c t a c g a c t c t g a c c g a a a g g t g t c g c g t c c g t g g a a a c t g t t c c c t t g g t t t t g t

480 (in frame with FLAG) GGPFGTIKHPAELAHSANNG GGPFGTIKHPAELAHSANNG ASGKSCDLVRSFQCMTGVIP g g c g g a c c c t t t g g a a c a a t c a a g c a c c c t g c t g a a c t g g c a c a t a g t g c c a a c a a t g g g c c g c c t g g g a a a c c t t g t t a g t t c g t g g g a c g a c t t g a c c g t g t a t c a c g g t t g t t a c c c

540 (in frame with FLAG) LDIAVRLLEPLKAEFPILSY LDIAVRLLEPLKAEFPILSY QVDGHPQQFRQFCLEWNQGV c t g g a c a t c g c c g t g c g g c t g c t g g a a c c t c t g a a a g c a g a g t t c c c a a t t c t g t c c t a c g a c c t g t a g c g g c a c g c c g a c g a c c t t g g a g a c t t t c g t c t c a a g g g t t a a g a c a g g a t g

PspOMIApaI 600 (in frame with FLAG) ADFYQLAGVVAVEVTGGPKV ADFYQLAGVVAVEVTGGPKV GIKILQCSHDSHLDSPAGLH g c c g a t t t t t a t c a g c t g g c a g g a g t g g t c g c t g t g g a g g t c a c t g g g g g c c c c a a g g t g c g g c t a a a a a t a g t c g a c c g t c c t c a c c a g c g a c a c c t c c a g t g a c c c c c g g g g t t c c a c

660 (in frame with FLAG) PFHPGREDKPEPPPEGRLPD PFHPGREDKPEPPPEGRLPD REVWSPLVFWFWRWLPAQWI c c t t t c c a c c c a g g a c g g g a g g a c a a a c c a g a a c c a c c t c c a g a g g g g c g c c t g c c a g a t g g a a a g g t g g g t c c t g c c c t c c t g t t t g g t c t t g g t g g a g g t c t c c c c g c g g a c g g t c t a

720 (in frame with FLAG) PTKGSDHLRDVFGKAMGLTD PTKGSDHLRDVFGKAMGLTD RCLAGVMQSIHKPFSHAQSV c c g a c a a a g g g c t c c g a c c a t c t g c g a g a t g t g t t t g g g a a a g c t a t g g g c c t g a c t g a c g g c t g t t t c c c g a g g c t g g t a g a c g c t c t a c a c a a a c c c t t t c g a t a c c c g g a c t g a c t g

EcoRV 780 (in frame with FLAG) QDIVALSGGHTIGAAHKERS QDIVALSGGHTIGAAHKERS LIDDCQRSPVGNAGSM c a g g a t a t c g t c g c a c t g t c t g g a g g g c a c a c c a t t g g c g c c g c t c a t a a g g a a a g g t c a g t c c t a t a g c a g c g t g a c a g a c c t c c c g t g t g g t a a c c g c g g c g a g t a t t c c t t t c c a g t

PpuMIKflI 840 (in frame with FLAG) GFEGPWTSNPLIFDNSYFTE GFEGPWTSNPLIFDNSYFTE g g c t t c g a g g g a c c c t g g a c a a g c a a c c c c c t g a t t t t c g a c a a t t c t t a c t t t a c t g a a c c g a a g c t c c c t g g g a c c t g t t c g t t g g g g g a c t a a a a g c t g t t a a g a a t g a a a t g a c t t

900 (in frame with FLAG) LLSGEKEGLLQLPSDKALLS LLSGEKEGLLQLPSDKALLS c t g c t g a g t g g a g a g a a g g a a g g g c t g c t g c a g c t g c c c a g c g a c a a a g c c c t g c t g t c c g a c g a c t c a c c t c t c t t c c t t c c c g a c g a c g t c g a c g g g t c g c t g t t t c g g g a c g a c a g g

AhdI 960 (in frame with FLAG) DPVFRPLVDKYAADEDAFFA DPVFRPLVDKYAADEDAFFA g a t c c c g t g t t c a g a c c t c t g g t c g a t a a g t a t g c a g c c g a c g a g g a t g c t t t t t t c g c a c t a g g g c a c a a g t c t g g a g a c c a g c t a t t c a t a c g t c g g c t g c t c c t a c g a a a a a a g c g t

1020 (in frame with FLAG) DYAEAHQKLSELGFADAKGS DYAEAHQKLSELGFADAKGS g a t t a c g c a g a a g c a c a t c a g a a g c t g t c a g a a c t g g g a t t t g c c g a c g c c a a g g g c t c g c t a a t g c g t c t t c g t g t a g t c t t c g a c a g t c t t g a c c c t a a a c g g c t g c g g t t c c c g a g c

BglIIPaeR7IXhoIBspEI 1080 (in frame with FLAG) GSTSGSGSGLRSRGGGGSGG GSTSGSGSGLRSRGGGGSGG g g c t c g a c c t c g g g c t c g g g c t c c g g a c t c a g a t c t c g a g g a g g t g g g g g a t c t g g t g g c c c g a g c t g g a g c c c g a g c c c g a g g c c t g a g t c t a g a g c t c c t c c a c c c c c t a g a c c a c c g

SacII 1140 (in frame with FLAG) GGSGGGGSMATATPVPPRMG GGSGGGGSMATATPVPPRMG g g a g g t t c a g g a g g t g g a g g a t c g a t g g c g a c t g c g a c c c c c g t g c c g c c g c g g a t g g g c c c t c c a a g t c c t c c a c c t c c t a g c t a c c g c t g a c g c t g g g g g c a c g g c g g c g c c t a c c c g

BlpI 1200 (in frame with FLAG) SRAGGPTTPLSPTRLSRLQE SRAGGPTTPLSPTRLSRLQE a g c c g c g c t g g c g g c c c c a c c a c g c c g c t g a g c c c c a c g c g c c t g t c g c g g c t c c a g g a g t c g g c g c g a c c g c c g g g g t g g t g c g g c g a c t c g g g g t g c g c g g a c a g c g c c g a g g t c c t c

BsmBIEsp3IBsrGI 1260 (in frame with FLAG) KEELRELNDRLAVYIDKVRS KEELRELNDRLAVYIDKVRS a a g g a g g a g c t g c g c g a g c t c a a t g a c c g g c t g g c g g t g t a c a t c g a c a a g g t g c g c a g c t t c c t c c t c g a c g c g c t c g a g t t a c t g g c c g a c c g c c a c a t g t a g c t g t t c c a c g c g t c g

PaqCI 1320 (in frame with FLAG) LETENSALQLQVTEREEVRG LETENSALQLQVTEREEVRG c t g g a g a c g g a g a a c a g c g c g c t g c a g c t g c a g g t g a c g g a g c g c g a g g a g g t g c g c g g c g a c c t c t g c c t c t t g t c g c g c g a c g t c g a c g t c c a c t g c c t c g c g c t c c t c c a c g c g c c g

1380 (in frame with FLAG) RELTGLKALYETELADARRA RELTGLKALYETELADARRA c g t g a g c t c a c c g g c c t c a a g g c g c t c t a c g a g a c c g a g c t g g c c g a c g c g c g a c g c g c g g c a c t c g a g t g g c c g g a g t t c c g c g a g a t g c t c t g g c t c g a c c g g c t g c g c g c t g c g c g c

1440 (in frame with FLAG) LDDTARERAKLQIELGKCKA LDDTARERAKLQIELGKCKA c t c g a c g a c a c g g c c c g c g a g c g c g c c a a g c t g c a g a t c g a g c t g g g c a a g t g c a a g g c g g a g c t g c t g t g c c g g g c g c t c g c g c g g t t c g a c g t c t a g c t c g a c c c g t t c a c g t t c c g c

1500 (in frame with FLAG) EHDQLLLNYAKKESDLNGAQ EHDQLLLNYAKKESDLNGAQ g a a c a c g a c c a g c t g c t c c t c a a c t a t g c t a a g a a g g a a t c t g a t c t t a a t g g c g c c c a g c t t g t g c t g g t c g a c g a g g a g t t g a t a c g a t t c t t c c t t a g a c t a g a a t t a c c g c g g g t c

BsgIEcoRIHindIII 1560 (in frame with FLAG) IKLREYEAALNSKDAALATA IKLREYEAALNSKDAALATA a t c a a g c t t c g a g a a t a t g a a g c a g c a c t g a a t t c g a a a g a t g c a g c t c t t g c t a c t g c a t a g t t c g a a g c t c t t a t a c t t c g t c g t g a c t t a a g c t t t c t a c g t c g a g a a c g a t g a c g t

1620 (in frame with FLAG) LGDKKSLEGDLEDLKDQIAQ LGDKKSLEGDLEDLKDQIAQ c t t g g t g a c a a a a a a a g t t t a g a g g g a g a t t t g g a g g a t c t g a a g g a t c a g a t t g c c c a g g a a c c a c t g t t t t t t t c a a a t c t c c c t c t a a a c c t c c t a g a c t t c c t a g t c t a a c g g g t c

1680 (in frame with FLAG) LEASLAAAKKQLADETLLKV LEASLAAAKKQLADETLLKV t t g g a a g c c t c c t t a g c t g c a g c c a a a a a a c a g t t a g c a g a t g a a a c t t t a c t t a a a g t a a a c c t t c g g a g g a a t c g a c g t c g g t t t t t t g t c a a t c g t c t a c t t t g a a a t g a a t t t c a t

EcoNI 1740 (in frame with FLAG) DLENRCQSLTEDLEFRKSMY DLENRCQSLTEDLEFRKSMY g a t t t g g a g a a t c g t t g t c a g a g c c t t a c t g a g g a c t t g g a g t t t c g c a a a a g c a t g t a t c t a a a c c t c t t a g c a a c a g t c t c g g a a t g a c t c c t g a a c c t c a a a g c g t t t t c g t a c a t a

1800 (in frame with FLAG) EEEINETRRKHETRLVEVDS EEEINETRRKHETRLVEVDS g a a g a g g a g a t t a a c g a g a c c a g a a g g a a g c a t g a a a c g c g c t t g g t a g a g g t g g a t t c t c t t c t c c t c t a a t t g c t c t g g t c t t c c t t c g t a c t t t g c g c g a a c c a t c t c c a c c t a a g a

1860 (in frame with FLAG) GRQIEYEYKLAQALHEMREQ GRQIEYEYKLAQALHEMREQ g g g c g t c a a a t t g a g t a t g a g t a c a a g c t g g c g c a a g c c c t t c a t g a g a t g a g a g a g c a a c c c g c a g t t t a a c t c a t a c t c a t g t t c g a c c g c g t t c g g g a a g t a c t c t a c t c t c t c g t t

1920 (in frame with FLAG) HDAQVRLYKEELEQTYHAKL HDAQVRLYKEELEQTYHAKL c a t g a t g c c c a a g t g a g g c t g t a t a a g g a g g a g c t g g a g c a g a c t t a c c a t g c c a a a c t t g t a c t a c g g g t t c a c t c c g a c a t a t t c c t c c t c g a c c t c g t c t g a a t g g t a c g g t t t g a a

1980 (in frame with FLAG) ENARLSSEMNTSTVNSAREE ENARLSSEMNTSTVNSAREE g a g a a t g c c a g a c t g t c a t c a g a g a t g a a t a c t t c t a c t g t c a a c a g t g c c a g g g a a g a a c t c t t a c g g t c t g a c a g t a g t c t c t a c t t a t g a a g a t g a c a g t t g t c a c g g t c c c t t c t t

2040 (in frame with FLAG) LMESRMRIESLSSQLSNLQK LMESRMRIESLSSQLSNLQK c t g a t g g a a a g c c g c a t g a g a a t t g a g a g c c t t t c a t c c c a g c t t t c t a a t c t a c a g a a a g a c t a c c t t t c g g c g t a c t c t t a a c t c t c g g a a a g t a g g g t c g a a a g a t t a g a t g t c t t t

2100 (in frame with FLAG) ESRACLERIQELEDLLAKEK ESRACLERIQELEDLLAKEK g a g t c t a g a g c a t g t t t g g a a a g g a t t c a a g a a t t a g a g g a c t t g c t t g c t a a a g a a a a a c t c a g a t c t c g t a c a a a c c t t t c c t a a g t t c t t a a t c t c c t g a a c g a a c g a t t t c t t t t t

2160 (in frame with FLAG) DNSRRMLTDKEREMAEIRDQ DNSRRMLTDKEREMAEIRDQ g a c a a c t c t c g t c g c a t g c t g a c a g a c a a a g a g a g a g a g a t g g c g g a a a t a a g g g a t c a a c t g t t g a g a g c a g c g t a c g a c t g t c t g t t t c t c t c t c t c t a c c g c c t t t a t t c c c t a g t t

2220 (in frame with FLAG) MQQQLNDYEQLLDVKLALDM MQQQLNDYEQLLDVKLALDM a t g c a g c a a c a g c t g a a t g a c t a t g a a c a g c t t c t t g a t g t a a a g t t a g c c c t g g a c a t g t a c g t c g t t g t c g a c t t a c t g a t a c t t g t c g a a g a a c t a c a t t t c a a t c g g g a c c t g t a c

2280 (in frame with FLAG) EISAYRKLLEGEEERLKLSP EISAYRKLLEGEEERLKLSP g a a a t c a g t g c t t a c a g g a a a c t c t t a g a a g g c g a a g a a g a g a g g t t g a a g c t g t c t c c a c t t t a g t c a c g a a t g t c c t t t g a g a a t c t t c c g c t t c t t c t c t c c a a c t t c g a c a g a g g t

2340 (in frame with FLAG) SPSSRVTVSRASSSRSVRTT SPSSRVTVSRASSSRSVRTT a g c c c t t c t t c c c g t g t g a c a g t a t c c c g a g c a t c c t c a a g t c g t a g t g t a c g t a c a a c t t c g g g a a g a a g g g c a c a c t g t c a t a g g g c t c g t a g g a g t t c a g c a t c a c a t g c a t g t t g a

2400 (in frame with FLAG) RGKRKRVDVEESEASSSVSI RGKRKRVDVEESEASSSVSI a g a g g a a a g c g g a a g a g g g t t g a t g t g g a a g a a t c a g a g g c g a g t a g t a g t g t t a g c a t c t c t c c t t t c g c c t t c t c c c a a c t a c a c c t t c t t a g t c t c c g c t c a t c a t c a c a a t c g t a g

BbvCI 2460 (in frame with FLAG) SHSASATGNVCIEEIDVDGK SHSASATGNVCIEEIDVDGK t c t c a t t c c g c c t c a g c c a c t g g a a a t g t t t g c a t c g a a g a a a t t g a t g t t g a t g g g a a a a g a g t a a g g c g g a g t c g g t g a c c t t t a c a a a c g t a g c t t c t t t a a c t a c a a c t a c c c t t t

2520 (in frame with FLAG) FIRLKNTSEQDQPMGGWEMI FIRLKNTSEQDQPMGGWEMI t t t a t c c g c t t g a a g a a c a c t t c t g a a c a g g a t c a a c c a a t g g g a g g c t g g g a g a t g a t c a a a t a g g c g a a c t t c t t g t g a a g a c t t g t c c t a g t t g g t t a c c c t c c g a c c c t c t a c t a g

2580 (in frame with FLAG) RKIGDTSVSYKYTSRYVLKA RKIGDTSVSYKYTSRYVLKA a g a a a a a t t g g a g a c a c a t c a g t c a g t t a t a a a t a t a c c t c a a g a t a t g t g c t g a a g g c a t c t t t t t a a c c t c t g t g t a g t c a g t c a a t a t t t a t a t g g a g t t c t a t a c a c g a c t t c c g t

2640 (in frame with FLAG) GQTVTIWAANAGVTASPPTD GQTVTIWAANAGVTASPPTD g g c c a g a c t g t t a c a a t t t g g g c t g c a a a c g c t g g t g t c a c a g c c a g c c c c c c a a c t g a c c c g g t c t g a c a a t g t t a a a c c c g a c g t t t g c g a c c a c a g t g t c g g t c g g g g g g t t g a c t g

2700 (in frame with FLAG) LIWKNQNSWGTGEDVKVILK LIWKNQNSWGTGEDVKVILK c t c a t c t g g a a g a a c c a g a a c t c g t g g g g c a c t g g c g a a g a t g t g a a g g t t a t a t t g a a a g a g t a g a c c t t c t t g g t c t t g a g c a c c c c g t g a c c g c t t c t a c a c t t c c a a t a t a a c t t t

2760 (in frame with FLAG) NSQGEEVAQRSTVFKTTIPE NSQGEEVAQRSTVFKTTIPE a a t t c t c a g g g a g a g g a g g t t g c t c a a a g a a g t a c a g t c t t t a a a a c a a c c a t a c c t g a a t t a a g a g t c c c t c t c c t c c a a c g a g t t t c t t c a t g t c a g a a a t t t t g t t g g t a t g g a c t t

2820 (in frame with FLAG) EEEEEEEAAGVVVEEELFHQ EEEEEEEAAGVVVEEELFHQ g a a g a g g a g g a g g a g g a a g a a g c a g c t g g a g t g g t t g t t g a g g a a g a a c t t t t c c a c c a g c t t c t c c t c c t c c t c c t t c t t c g t c g a c c t c a c c a a c a a c t c c t t c t t g a a a a g g t g g t c

BamHI 2880 (in frame with FLAG) QGTPRASNRSCAIM* QGTPRASNRSCAIM* c a g g g a a c c c c a a g a g c a t c c a a t a g a a g c t g t g c a a t t a t g t a a g g a t c c a c c g g a t c t g t c c c t t g g g g t t c t c g t a g g t t a t c t t c g a c a c g t t a a t a c a t t c c t a g g t g g c c t a g a

2940 a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g

HpaIMfeI 3000 SV40 poly(A) signal c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c

3060 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t

AflIIBtsαIBtsI 3120 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g

3180 f1 ori g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g

3240 f1 ori a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t

3300 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g

3360 f1 ori c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g

3420 f1 ori c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c

3480 f1 ori A G G G A A G A A F1ori-R c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t

3540 f1 ori A G C G A A A G G A G F1ori-R a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g

3600 f1 ori c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t

3660 AmpR promoter t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a

3720 AmpR promoter g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t

3780 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t

3840 SV40 promoter SV40 promoter g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a

3900 SV40 promoter SV40 promoter SV40 ori c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t

SfiI 3960 SV40 promoter SV40 promoter SV40 ori a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c

StuI 4020 SV40 promoter SV40 promoter SV40 ori t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c

4080 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t

4140 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a

4200 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a

MscI 4260 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c

Tth111IPflFI 4320 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a

4380 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g

BsrDI 4440 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g

4500 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t

4560 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR LDEEHQGLAPAELFARLKAS QVFLMLPERWGFQEGPELRA t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c

4620 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a

RsrII 4680 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c

4740 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g

4800 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t

4860 260 265 RLLDEFF* NeoR/KanR 260 265 RLLDEFF* NeoR/KanR MTDQA RLLDEFF* AKKVLEESRSEPTRFSRGLS t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c

4920 TPNLPSRDFDSTAAFYERLG AWGAM a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g

4980 FGIVFRDAGWMILQRGDLML t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c

5040 A C T T T G T G C C T T C C T C T G T T TK-pA-R EFFAHPRGRLTETRKETIPE g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t

5100 HSV TK poly(A) signal GTRAMTAIKRQNKTHGVGSF g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a

5160 HSV TK poly(A) signal VHKRGVRSQGWHSVDTPPRP g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g

5220 HWGQYARVSSFSPPHPPSSG c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a

5280 EGPGLAANVGAAGPAIASGY g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g

5340 SYIL* t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c

5400 a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c

5460 ori t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g

5520 ori t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c

5580 ori c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a

5640 ori c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g

5700 ori c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g

5760 ori g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a

ApaLI 5820 ori t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a

5880 ori g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g

5940 ori G G G A A A C G C C T G G T A T C T T pBR322ori-F g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a

6000 ori T pBR322ori-F t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t

6060 ori g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a

AflIIIPciI 6120 t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a

AseI 6180 a t t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c t a a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g

6240 CMV enhancer a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t

6300 CMV enhancer c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g

6360 CMV enhancer t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t

NdeI 6420 CMV enhancer a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c

6480 CMV enhancer g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a

3ʹ 5ʹ 6510 CMV enhancer CMV promoter a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.