Addgene pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE) Sequencing Result

Map View

T7 (8848 .. 9) M13 fwd M13 Forward (8821 .. 8838) M13/pUC Forward (8807 .. 8829) F1ori-R (8574 .. 8593) F1ori-F (8362 .. 8383) Amp-R (7857 .. 7876) ScaI (7787) BsaI (7368) AhdI (7307) pBR322ori-F (6555 .. 6574) L4440 (6304 .. 6321) CAP binding site M13/pUC Reverse (6086 .. 6108) lac operator M13 Reverse (6073 .. 6089) M13 rev T3 (6034 .. 6054) PmeI (6001) piggyBac right (3') inverted repeat SpeI (5739) AgeI (5718) StuI * (5402) EF-1α intron A BstAPI (5039) BbvCI (4911) EF1a-F (4671 .. 4691) MscI (4607) MauBI (4570) SmaI (4520) AscI (19) piggyBac left (5') inverted repeat NsiI (74) NheI (356) BmtI (360) AfeI (361) tet operator tet operator tet operator tet operator tet operator tet operator tet operator LNCX (670 .. 694) EcoRI (762) NcoI (769) LucNrev (837 .. 855) BstEII (1378) Bsu36I (1384) PpuMI (1950) BamHI (2424) MluI (2580) SV40pA-R (2621 .. 2640) EBV-rev (2675 .. 2694) NdeI (2748) WPRE-R (3283 .. 3303) SbfI (3391) BsaBI * (3481) BlpI (3890) PmlI (4032) T2A TspMI - XmaI (4518) pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE) 8858 bp

Sequence View

AscI 5ʹ 3ʹ 60 T7 promoter piggyBac left (5') inverted repeat A C T A T A G G G T7 a c t a t a g g g c g a a t t g g g g c g c g c c a t t c t a g a t t a a c c c t a g a a a g a t a g t c t g c g t a a t g a t a t c c c g c t t a a c c c c g c g c g g t a a g a t c t a a t t g g g a t c t t t c t a t c a g a c g c a t t

NsiI 120 piggyBac left (5') inverted repeat a a t t g a c g c a t g c a t t c t t g a a a t a t t g c t c t c t c t t t c t a a a t a g c g c g a a t c c g t c g c t t a a c t g c g t a c g t a a g a a c t t t a t a a c g a g a g a g a a a g a t t t a t c g c g c t t a g g c a g c g

180 t g t g c a t t t a g g a c a t c t c a g t c g c c g c t t g g a g c t c c c g t g a g g c g t g c t t g t c a a t g c a c a c g t a a a t c c t g t a g a g t c a g c g g c g a a c c t c g a g g g c a c t c c g c a c g a a c a g t t a c g

240 g g t a a g t g t c a c t g a t t t t g a a c t a t a a c g a c c g c g t g a g t c a a a a t g a c g c a t g a t t a t c c a t t c a c a g t g a c t a a a a c t t g a t a t t g c t g g c g c a c t c a g t t t t a c t g c g t a c t a a t a

300 c t t t t a c g t g a c t t t t a a g a t t t a a c t c a t a c g a t a a t t a t a t t g t t a t t t c a t g t t c t a g a a a a t g c a c t g a a a a t t c t a a a t t g a g t a t g c t a t t a a t a t a a c a a t a a a g t a c a a g a t

BmtINheI 360 c t t a c g t g a t a a c t t a t t a t a t a t a t a t t t t c t t g t t a t a g a t a t c a a c t a g a a t g c t a g g a a t g c a c t a t t g a a t a a t a t a t a t a t a a a a g a a c a a t a t c t a t a g t t g a t c t t a c g a t c

AfeI 420 tet operator c g c t g a t a t c t c c a c c c c a c a g t g g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t g a a g a g c g a c t a t a g a g g t g g g g t g t c a c c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a c t t c t

480 tet operator tet operator g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t g c a g a c t t t a c t c c c t a t c a g t g a t a g a g c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a c g t c t g a a a t g a g g g a t a g t c a c t a t c t c

540 tet operator tet operator tet operator a a c g t a t a a g g a g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t g a c c a g t t t a c t c c c t a t t g c a t a t t c c t c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a c t g g t c a a a t g a g g g a t

600 tet operator tet operator t c a g t g a t a g a g a a c g t a t c t a c a g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t a t c c a a g t c a c t a t c t c t t g c a t a g a t g t c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a t a g g t

660 tet operator g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t a a g c t t t a g g c g t g t a c g g t g g g c g c c t a c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a t t c g a a a t c c g c a c a t g c c a c c c g c g g a t

720 A G C T C G T T T A G T G A A C C G T C A G A T C LNCX t a a a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a g c a a t t c c a c a a c a c t t t t a t t t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c t c g t t a a g g t g t t g t g a a a a

NcoIEcoRI 780 1 MED luciferase MED g t c t t a t a c c a a c t t t c c g t a c c a c t t c c t a c c c t c g t a a a g a a t t c a c c a t g g a a g a c g c a g a a t a t g g t t g a a a g g c a t g g t g a a g g a t g g g a g c a t t t c t t a a g t g g t a c c t t c t g c

840 5 10 15 20 AKNIKKGPAPFYPLEDGTAG luciferase C C T C LucNrev AKNIKKGPAPFYPLEDGTAG c c a a a a a c a t a a a g a a a g g c c c g g c g c c a t t c t a t c c a c t a g a g g a t g g a a c c g c t g g a g g g t t t t t g t a t t t c t t t c c g g g c c g c g g t a a g a t a g g t g a t c t c c t a c c t t g g c g a c c t c

900 25 30 35 40 EQLHKAMKRYALVPGTIAFT luciferase T C G T T G A C G T A T T C C LucNrev EQLHKAMKRYALVPGTIAFT a g c a a c t g c a t a a g g c t a t g a a g a g a t a c g c c c t g g t t c c t g g a a c a a t t g c t t t t a c a g t c g t t g a c g t a t t c c g a t a c t t c t c t a t g c g g g a c c a a g g a c c t t g t t a a c g a a a a t g t c

960 45 50 55 60 DAHIEVNITYAEYFEMSVRL luciferase DAHIEVNITYAEYFEMSVRL a t g c a c a t a t c g a g g t g a a c a t c a c g t a c g c g g a a t a c t t c g a a a t g t c c g t t c g g t t g g t a c g t g t a t a g c t c c a c t t g t a g t g c a t g c g c c t t a t g a a g c t t t a c a g g c a a g c c a a c c

1020 65 70 75 80 AEAMKRYGLNTNHRIVVCSE luciferase AEAMKRYGLNTNHRIVVCSE c a g a a g c t a t g a a a c g a t a t g g g c t g a a t a c a a a t c a c a g a a t c g t c g t a t g c a g t g a a a g t c t t c g a t a c t t t g c t a t a c c c g a c t t a t g t t t a g t g t c t t a g c a g c a t a c g t c a c t t t

1080 85 90 95 100 NSLQFFMPVLGALFIGVAVA luciferase NSLQFFMPVLGALFIGVAVA a c t c t c t t c a a t t c t t t a t g c c g g t g t t g g g c g c g t t a t t t a t c g g a g t t g c a g t t g c g c t g a g a g a a g t t a a g a a a t a c g g c c a c a a c c c g c g c a a t a a a t a g c c t c a a c g t c a a c g c g

1140 105 110 115 120 PANDIYNERELLNSMNISQP luciferase PANDIYNERELLNSMNISQP c c g c g a a c g a c a t t t a t a a t g a a c g t g a a t t g c t c a a c a g t a t g a a c a t t t c g c a g c c t a g g c g c t t g c t g t a a a t a t t a c t t g c a c t t a a c g a g t t g t c a t a c t t g t a a a g c g t c g g a t

1200 125 130 135 140 TVVFVSKKGLQKILNVQKKL luciferase TVVFVSKKGLQKILNVQKKL c c g t a g t g t t t g t t t c c a a a a a g g g g t t g c a a a a a a t t t t g a a c g t g c a a a a a a a a t t a c g g c a t c a c a a a c a a a g g t t t t t c c c c a a c g t t t t t t a a a a c t t g c a c g t t t t t t t t a a t g

1260 145 150 155 160 PIIQKIIIMDSKTDYQGFQS luciferase PIIQKIIIMDSKTDYQGFQS c a a t a a t c c a g a a a a t t a t t a t c a t g g a t t c t a a a a c g g a t t a c c a g g g a t t t c a g t c g a g t t a t t a g g t c t t t t a a t a a t a g t a c c t a a g a t t t t g c c t a a t g g t c c c t a a a g t c a g c t

1320 165 170 175 180 MYTFVTSHLPPGFNEYDFVP luciferase MYTFVTSHLPPGFNEYDFVP t g t a c a c g t t c g t c a c a t c t c a t c t a c c t c c c g g t t t t a a t g a a t a c g a t t t t g t a c c a g a c a t g t g c a a g c a g t g t a g a g t a g a t g g a g g g c c a a a a t t a c t t a t g c t a a a a c a t g g t c

BstEII 1380 185 190 195 200 ESFDRDKTIALIMNSSGSTG luciferase ESFDRDKTIALIMNSSGSTG a g t c c t t t g a t c g t g a c a a a a c a a t t g c a c t g a t a a t g a a c t c c t c t g g a t c t a c t g g g t t c a g g a a a c t a g c a c t g t t t t g t t a a c g t g a c t a t t a c t t g a g g a g a c c t a g a t g a c c c a

Bsu36I 1440 205 210 215 220 LPKGVALPHRTACVRFSHAR luciferase LPKGVALPHRTACVRFSHAR t a c c t a a g g g t g t g g c c c t t c c g c a t a g a a c t g c c t g c g t c a g a t t c t c g c a t g c c a g a g a t g g a t t c c c a c a c c g g g a a g g c g t a t c t t g a c g g a c g c a g t c t a a g a g c g t a c g g t c t c

1500 225 230 235 240 DPIFGNQIIPDTAILSVVPF luciferase DPIFGNQIIPDTAILSVVPF a t c c t a t t t t t g g c a a t c a a a t c a t t c c g g a t a c t g c g a t t t t a a g t g t t g t t c c a t t c c t a g g a t a a a a a c c g t t a g t t t a g t a a g g c c t a t g a c g c t a a a a t t c a c a a c a a g g t a a g g

1560 245 250 255 260 HHGFGMFTTLGYLICGFRVV luciferase HHGFGMFTTLGYLICGFRVV a t c a c g g t t t t g g a a t g t t t a c t a c a c t c g g a t a t t t g a t a t g t g g a t t t c g a g t c g t c t t a g t g c c a a a a c c t t a c a a a t g a t g t g a g c c t a t a a a c t a t a c a c c t a a a g c t c a g c a g a

1620 265 270 275 280 LMYRFEEELFLRSLQDYKIQ luciferase LMYRFEEELFLRSLQDYKIQ t a a t g t a t a g a t t t g a a g a a g a g c t g t t t t t a c g a t c c c t t c a g g a t t a c a a a a t t c a a a a t t a c a t a t c t a a a c t t c t t c t c g a c a a a a a t g c t a g g g a a g t c c t a a t g t t t t a a g t t t

1680 285 290 295 300 SALLVPTLFSFFAKSTLIDK luciferase SALLVPTLFSFFAKSTLIDK g t g c g t t g c t a g t a c c a a c c c t a t t t t c a t t c t t c g c c a a a a g c a c t c t g a t t g a c a a a t c a c g c a a c g a t c a t g g t t g g g a t a a a a g t a a g a a g c g g t t t t c g t g a g a c t a a c t g t t t a

1740 305 310 315 320 YDLSNLHEIASGGAPLSKEV luciferase YDLSNLHEIASGGAPLSKEV a c g a t t t a t c t a a t t t a c a c g a a a t t g c t t c t g g g g g c g c a c c t c t t t c g a a a g a a g t c g t g c t a a a t a g a t t a a a t g t g c t t t a a c g a a g a c c c c c g c g t g g a g a a a g c t t t c t t c a g c

1800 325 330 335 340 GEAVAKRFHLPGIRQGYGLT luciferase GEAVAKRFHLPGIRQGYGLT g g g a g g c g g t t g c a a a a c g c t t c c a t c t t c c a g g g a t a c g a c a a g g a t a t g g g c t c a c t g c c c t c c g c c a a c g t t t t g c g a a g g t a g a a g g t c c c t a t g c t g t t c c t a t a c c c g a g t g a c

1860 345 350 355 360 ETTSAILITPEGDDKPGAVG luciferase ETTSAILITPEGDDKPGAVG a g a c t a c a t c a g c t a t t c t g a t t a c a c c c g a g g g g g a t g a t a a a c c g g g c g c g g t c g g t a t c t g a t g t a g t c g a t a a g a c t a a t g t g g g c t c c c c c t a c t a t t t g g c c c g c g c c a g c c a t

1920 365 370 375 380 KVVPFFEAKVVDLDTGKTLG luciferase KVVPFFEAKVVDLDTGKTLG a a g t t g t t c c a t t t t t t g a a g c g a a g g t t g t g g a t c t g g a t a c c g g g a a a a c g c t g g g c g t t c a a c a a g g t a a a a a a c t t c g c t t c c a a c a c c t a g a c c t a t g g c c c t t t t g c g a c c c g c

PpuMI 1980 385 390 395 400 VNQRGELCVRGPMIMSGYVN luciferase VNQRGELCVRGPMIMSGYVN t t a a t c a g a g a g g c g a a t t a t g t g t c a g a g g a c c t a t g a t t a t g t c c g g t t a t g t a a a c a a a t t a g t c t c t c c g c t t a a t a c a c a g t c t c c t g g a t a c t a a t a c a g g c c a a t a c a t t t g t

2040 405 410 415 420 NPEATNALIDKDGWLHSGDI luciferase NPEATNALIDKDGWLHSGDI a t c c g g a a g c g a c c a a c g c c t t g a t t g a c a a g g a t g g a t g g c t a c a t t c t g g a g a c a t a g t a g g c c t t c g c t g g t t g c g g a a c t a a c t g t t c c t a c c t a c c g a t g t a a g a c c t c t g t a t c

2100 425 430 435 440 AYWDEDEHFFIVDRLKSLIK luciferase AYWDEDEHFFIVDRLKSLIK c t t a c t g g g a c g a a g a c g a a c a c t t c t t c a t a g t t g a c c g c t t g a a g t c t t t a a t t a a a t g a a t g a c c c t g c t t c t g c t t g t g a a g a a g t a t c a a c t g g c g a a c t t c a g a a a t t a a t t t a

2160 445 450 455 460 YKGYQVAPAELESILLQHPN luciferase YKGYQVAPAELESILLQHPN a c a a a g g a t a c c a g g t g g c c c c c g c t g a a t t g g a g t c g a t a t t g t t a c a a c a c c c c a a c a t g t t t c c t a t g g t c c a c c g g g g g c g a c t t a a c c t c a g c t a t a a c a a t g t t g t g g g g t t g t

2220 465 470 475 480 IFDAGVAGLPDDDAGELPAA luciferase IFDAGVAGLPDDDAGELPAA t c t t c g a c g c g g g c g t g g c a g g t c t t c c c g a c g a t g a c g c c g g t g a a c t t c c c g c c g c c g a g a a g c t g c g c c c g c a c c g t c c a g a a g g g c t g c t a c t g c g g c c a c t t g a a g g g c g g c g g c

2280 485 490 495 500 VVVLEHGKTMTEKEIVDYVA luciferase VVVLEHGKTMTEKEIVDYVA t t g t t g t t t t g g a g c a c g g a a a g a c g a t g a c g g a a a a a g a g a t c g t g g a t t a c g t c g c c a a a c a a c a a a a c c t c g t g c c t t t c t g c t a c t g c c t t t t t c t c t a g c a c c t a a t g c a g c g g t

2340 505 510 515 520 SQVTTAKKLRGGVVFVDEVP luciferase SQVTTAKKLRGGVVFVDEVP g t c a a g t a a c a a c c g c g a a a a a g t t g c g c g g a g g a g t t g t g t t t g t g g a c g a a g t a c c g a c a g t t c a t t g t t g g c g c t t t t t c a a c g c g c c t c c t c a a c a c a a a c a c c t g c t t c a t g g c t

2400 525 530 535 540 KGLTGKLDARKIREILIKAK luciferase KGLTGKLDARKIREILIKAK a a g g t c t t a c c g g a a a a c t c g a c g c a a g a a a a a t c a g a g a g a t c c t c a t a a a g g c c a a g a t t c c a g a a t g g c c t t t t g a g c t g c g t t c t t t t t a g t c t c t c t a g g a g t a t t t c c g g t t c t

BamHI 2460 545 550 KGGKSKL* luciferase KGGKSKL* a g g g c g g a a a g t c c a a a t t g t a a g g a t c c t t t c t t t c t t t g a a t t t c t a c t a a g t g t a g a t c c c g c c t t t c a g g t t t a a c a t t c c t a g g a a a g a a a g a a a c t t a a a g a t g a t t c a c a t c t

2520 t a g a t g a g a t g g t g a c a g a t a g g c a a t t t c t a c t a a g t g t a g a t a g a t g a g a t g g t g a c a a t c t a c t c t a c c a c t g t c t a t c c g t t a a a g a t g a t t c a c a t c t a t c t a c t c t a c c a c t g t

2580 g a t a g g c a a t t t c t a c t a a g t g t a g a t c g a g t t t t c t t t t t g t t t t g t a c a a g t a a g t t a c t a t c c g t t a a a g a t g a t t c a c a t c t a g c t c a a a a g a a a a a c a a a a c a t g t t c a t t c a a t

MluI 2640 C G T T A T C G T A G T G T T T A A A G SV40pA-R c g c g t c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c g c g c a g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g

2700 G T G G T T T G T C C A A A C T C A T C EBV-rev a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t

NdeI 2760 *GDYMHRRY *VYTSP t c t t a t c a t g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c g t c a t a c a t a t g t c g a c g g t a g a a t a g t a c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g c a g t a t g t a t a c a g c t g c c a

2820 WPRE RHPSAAWLSIRSTQPRLRLR ISAPLRGLPLDSEDSPSPSS a t c g a t g c g g g g a g g c g g c c c a a a g g g a g a t c c g a c t c g t c t g a g g g c g a a g g c g a a g a c t a g c t a c g c c c c t c c g c c g g g t t t c c c t c t a g g c t g a g c a g a c t c c c g c t t c c g c t t c t g

2880 WPRE 1 IEG Factor Xa site PLPRLAPLLGRSPLDAPNLA ASSAASGAAPRPFSPGSSQP g c g g a a g a g g c c g c a g a g c c g g c a g c a g g c c g c g g g a a g g a a g g t c c g c t g g a t t g a g g g c g c c t t c t c c g g c g t c t c g g c c g t c g t c c g g c g c c c t t c c t t c c a g g c g a c c t a a c t c c c

2940 WPRE R (in frame with Factor Xa site) RDVAEGRPRAESRWQHRRA Factor Xa site SPVYCFSTGACFGPPLVPSC GFPRLLLVDGRLIWTAVCAL c c g a a g g g a c g t a g c a g a a g g a c g t c c c c g c g c a g a a t c c a g g t g g c a a c a c a g g c g a g c g g c t t c c c t g c a t c g t c t t c c t g c a g g g g c g c g t c t t a g g t c c a c c g t t g t g t c c g c t c g

3000 WPRE (in frame with Factor Xa site) AKERTMISPTTPRNCQCPTA GLFPRHNGRCCWPITLAWCG LWPFSSSKGSLVVSNDTGLL a g c c a a g g a a a g g a c g a t g a t t t c c c c g a c a a c a c c a c g g a a t t g t c a g t g c c c a a c a g c t c g g t t c c t t t c c t g c t a c t a a a g g g g c t g t t g t g g t g c c t t a a c a g t c a c g g g t t g t c g

3060 WPRE (in frame with Factor Xa site) EPLSSSGQGRRR* LGQGAAPLAPPSSNRRPLLS RAGTWCRALCAAILEATAIP c g a g c c c c t g t c c a g c a g c g g g c a a g g c a g g c g g c g a t g a g t t c c g c c g t g g c a a t a g g g g c t c g g g g a c a g g t c g t c g c c c g t t c c g t c c g c c g c t a c t c a a g g c g g c a c c g t t a t c c c

3120 WPRE PSLSLGPFSSVPPPLAGVLP LPFAFTGSLLQCTTAIGWGT a g g g g g a a a g c g a a a g t c c c g g a a a g g a g c t g a c a g g t g g t g g c a a t g c c c c a a c c a g t g t c c c c c t t t c g c t t t c a g g g c c t t t c c t c g a c t g t c c a c c a c c g t t a c g g g g t t g g t c a c

3180 WPRE PQTLLCLACWAVNGSLPGCS PTADAFVTCVVGRQRVVPWL g g g g t t g c g t c a g c a a a c a c a g t g c a c a c c a c g c c a c g t t g c c t g a c a a c g g g c c a c a a c c c c c a a c g c a g t c g t t t g t g t c a c g t g t g g t g c g g t g c a a c g g a c t g t t g c c c g g t g t t g

3240 WPRE RMFLCCGPNICPPSFSLWVP EEYLSLLWSKYLSSFIFAMR t c c t c a t a a a g a g a c a g c a a c c a g g a t t t a t a c a a g g a g g a g a a a a t g a a a g c c a t a c g g a g g a g t a t t t c t c t g t c g t t g g t c c t a a a t a t g t t c c t c c t c t t t t a c t t t c g g t a t g c c

3300 WPRE C A T A G C G T A A A A G G A G C A WPRE-R LLLM SAIAHYLPM g a a g c a a t a g c a t g a t a c a a a g g c a t t a a a g c a g c g t a t c c a c a t a g c g t a a a a g g a g c a c t t c g t t a t c g t a c t a t g t t t c c g t a a t t t c g t c g c a t a g g t g t a t c g c a t t t t c c t c g t

3360 WPRE A C A WPRE-R a c a t a g t t a a g a a t a c c a g t c a a t c t t t c a c a a a t t t t g t a a t c c a g a g g t t g a t t a t c g t g t a t c a a t t c t t a t g g t c a g t t a g a a a g t g t t t a a a a c a t t a g g t c t c c a a c t a a t a g c

SbfI 3420 * Tet-On® 3G * a t a a g c t t g a t a t c a a g c t t g c a t g c c t g c a g g t c g a c t c t a g a g t c t t a t g c g g c c t t a t a t t c g a a c t a t a g t t c g a a c g t a c g g a c g t c c a g c t g a g a t c t c a g a a t a c g c c g g a a t

3480 245 240 235 230 GPLMDLDFDDLADAPLMDLD Tet-On® 3G GPLMDLDFDDLADAPLMDLD g c c t g g c a g c a t a t c g a g a t c g a a g t c a t c g a g a g c g t c a g c a g g g a g c a t a t c c a g a t c c g g a c c g t c g t a t a g c t c t a g c t t c a g t a g c t c t c g c a g t c g t c c c t c g t a t a g g t c t a g

BsaBI* 3540 225 220 215 210 FDDLADAPLMDLDFDDLADT Tet-On® 3G FDDLADAPLMDLDFDDLADT a a a a t c a t c c a g g g c a t c g g c g g g c a g c a t g t c c a g g t c g a a g t c g t c c a g g g c g t c g g t t t t t a g t a g g t c c c g t a g c c g c c c g t c g t a c a g g t c c a g c t t c a g c a g g t c c c g c a g c c a

3600 205 200 195 190 PGGSECKLQKELGCIILELG Tet-On® 3G PGGSECKLQKELGCIILELG g g g t c c g c c g c t c t c g c a c t t c a g c t g c t t c t c g a g g c c g c a g a t g a t c a g t t c c a g g c c c c c a g g c g g c g a g a g c g t g a a g t c g a c g a a g a g c t c c g g c g t c t a c t a g t c a a g g t c c g g

3660 185 180 175 170 FLFAPEAGQRDFLEIAQKLL Tet-On® 3G FLFAPEAGQRDFLEIAQKLL g a a c a g g a a g g c g g g c t c g g c g c c c t g t c t g t c g a a c a g c t c g a t g g c c t g c t t c a g c a g c t t g t c c t t c c g c c c g a g c c g c g g g a c a g a c a g c t t g t c g a g c t a c c g g a c g a a g t c g t c

3720 165 160 155 150 PPMSDTTPTEREEKAVQHEQ Tet-On® 3G PPMSDTTPTEREEKAVQHEQ g g g g g g c a t g c t g t c g g t g g t g g g t g t c t c t c t t t c c t c t t t g g c g a c c t g g t g t t c c t g c c c c c c g t a c g a c a g c c a c c a c c c a c a g a g a g a a a g g a g a a a c c g c t g g a c c a c a a g g a c

3780 145 140 135 130 EELVCGLTFHGVASLAYLAN Tet-On® 3G EELVCGLTFHGVASLAYLAN t t c t t c c a g c a c g c a g c c c a g g g t g a a g t g g c c c a c g g c g c t c a g g g c g t a c a g g g c g t t a a g a a g g t c g t g c g t c g g g t c c c a c t t c a c c g g g t g c c g c g a g t c c c g c a t g t c c c g c a a

3840 125 120 115 110 ELSFGQQCLFALQNELTEYQ Tet-On® 3G ELSFGQQCLFALQNELTEYQ t t c c a g g c t g a a g c c c t g c t g g c a c a g g a a g g c c a g c t g g t t t t c c a g t g t c t c g t a c t g a a g g t c c g a c t t c g g g a c g a c c g t g t c c t t c c g g t c g a c c a a a a g g t c a c a g a g c a t g a c

BlpI 3900 105 100 95 90 KETPRTGLHVKAGDRHSLLA Tet-On® 3G KETPRTGLHVKAGDRHSLLA c t t c t c g g t g g g t c t g g t g c c c a g g t g c a c t t t g g c g c c g t c c c g g t g g c t c a g c a g g g c g a a g a g c c a c c c a g a c c a c g g g t c c a c g t g a a a c c g c g g c a g g g c c a c c g a g t c g t c c c g

3960 85 80 75 70 CRYSKANNRLFDQWSEGELP Tet-On® 3G CRYSKANNRLFDQWSEGELP g c a t c t g t a g c t c t t g g c g t t g t t c c g c a g g a a g t c c t g c c a g c t c t c g c c t t c c a g a g g c g t a g a c a t c g a g a a c c g c a a c a a g g c g t c c t t c a g g a c g g t c g a g a g c g g a a g g t c t c c

4020 65 60 55 50 CSHTHHRDLMEIPLADLLAR Tet-On® 3G MGRASSRAR CSHTHHRDLMEIPLADLLAR g c a g c t g t g g g t g t g g t g c c g g t c c a g c a t c t c g a t g g g c a g g g c g t c c a g c a g g g c c c g c g t c g a c a c c c a c a c c a c g g c c a g g t c g t a g a g c t a c c c g t c c c g c a g g t c g t c c c g g g c

PmlI 4080 45 40 35 30 KNKVHWYLTPQEVGLKQALK Tet-On® 3G LFFTCQYRVGCSTPSFWASF KNKVHWYLTPQEVGLKQALK c t t g t t c t t c a c g t g c c a g t a c a g g g t g g g c t g t t c c a c g c c c a g t t t c t g g g c c a g c t t g a a c a a g a a g t g c a c g g t c a t g t c c c a c c c g a c a a g g t g c g g g t c a a a g a c c c g g t c g a a

4140 25 20 15 10 RTTLGEIGVGNLLELASNIV Tet-On® 3G RVVRPSMPTPFSSSRALLIT RTTLGEIGVGNLLELASNIV c c g g g t g g t c a g g c c c t c g a t g c c c a c g c c g t t c a g c a g t t c c a g g g c g c t g t t g a t c a c g g c c c a c c a g t c c g g g a g c t a c g g g t g c g g c a a g t c g t c a a g g t c c c g c g a c a a c t a g t g

4200 5 1 KSKDLRSM SGGG 155 150 PGPNEEVD Tet-On® 3G T2A LLLSSRLIDPPPGPGFSSTS KSKDLRSMSGGGPGPNEEVD t t t g c t c t t g t c c a g c c g g c t c a t c g a c c c t c c g c c t g g g c c a g g a t t c t c c t c g a c g t c a a a c g a g a a c a g g t c g g c c g a g t a g c t g g g a g g c g g a c c c g g t c c t a a g a g g a g c t g c a g

4260 145 140 GCTLLSGRGE SGG 120 DQEEAVF T2A BleoR PHVSRLPLPSEPPSCSSATK GCTLLSGRGESGGDQEEAVF a c c g c a t g t t a g c a g a c t t c c t c t g c c c t c g g a a c c g c c g t c c t g c t c c t c g g c c a c g a a t g g c g t a c a a t c g t c t g a a g g a g a c g g g a g c c t t g g c g g c a g g a c g a g g a g c c g g t g c t t

4320 115 110 105 100 HVCNGAPDRLAFERGWPQEG BleoR CTQLPAGSRRANSRPHGCSP HVCNGAPDRLAFERGWPQEG g t g c a c g c a g t t g c c g g c c g g g t c g c g c a g g g c g a a c t c c c g c c c c c a c g g c t g c t c g c c c a c g t g c g t c a a c g g c c g g c c c a g c g c g t c c c g c t t g a g g g c g g g g g t g c c g a c g a g c g g

4380 95 90 85 80 IETMAPGSADRFNTSVVESW BleoR ISVMAGPEASRKFVDTTSDH IETMAPGSADRFNTSVVESW g a t c t c g g t c a t g g c c g g c c c g g a g g c g t c c c g g a a g t t c g t g g a c a c g a c c t c c g a c c a c t a g a g c c a g t a c c g g c c g g g c c t c c g c a g g g c c t t c a a g c a c c t g t g c t g g a g g c t g g t

4440 75 70 65 60 EAYLEDLGRVWVWALTNDPV BleoR SAYSSSRPRTHTQARVLSGT EAYLEDLGRVWVWALTNDPV c t c g g c g t a c a g c t c g t c c a g g c c g c g c a c c c a c a c c c a g g c c a g g g t g t t g t c c g g c a c g a g c c g c a t g t c g a g c a g g t c c g g c g c g t g g g t g t g g g t c c g g t c c c a c a a c a g g c c g t g

4500 55 50 45 40 VQDQVASIFLTVDDRVVGAF BleoR TWSWTALMNRVTSSRTTPAK VQDQVASIFLTVDDRVVGAF c a c c t g g t c c t g g a c c g c g c t g a t g a a c a g g g t c a c g t c g t c c c g g a c c a c a c c g g c g a a g t g g a c c a g g a c c t g g c g c g a c t a c t t g t c c c a g t g c a g c a g g g c c t g g t g t g g c c g c t t

TspMIXmaISmaI 4560 35 30 25 20 DDEVFDRSFGLRDTWFEVAG BleoR SSSTKSRENPSRSVQNSTAP DDEVFDRSFGLRDTWFEVAG g t c g t c c t c c a c g a a g t c c c g g g a g a a c c c g a g c c g g t c g g t c c a g a a c t c g a c c g c t c c c a g c a g g a g g t g c t t c a g g g c c c t c t t g g g c t c g g c c a g c c a g g t c t t g a g c t g g c g a g g

MscIMauBI 4620 15 10 5 1 AVDRATLVPVASTLKAM BleoR ATSRAVSTGTALVNLAIILE AVDRATLVPVASTLKAM g g c g a c g t c g c g c g c g g t g a g c a c c g g a a c g g c a c t g g t c a a c t t g g c c a t a a t t c t c g a c c g c t g c a g c g c g c g c c a c t c g t g g c c t t g c c g t g a c c a g t t g a a c c g g t a t t a a g a g c t

4680 EF-1 α intron A C T T G G T G A C A EF1a-F SSRSPVHDT* g a g c t c c a g a t c c c c t g t t c a c g a c a c c t g a a a t g g a a g a a a a a a a c t t t g a a c c a c t g t c t c g a g g t c t a g g g g a c a a g t g c t g t g g a c t t t a c c t t c t t t t t t t g a a a c t t g g t g a c a

4740 EF-1 α intron A G A C T C C G A A C T EF1a-F c t g a g g c t t g a g a a t g a a c c a a g a t c c a a a c t c a a a a a g g c c a a a t t c c a a g g a g a a t t a g a c t c c g a a c t c t t a c t t g g t t c t a g g t t t g a g t t t t t c c g g t t t a a g g t t c c t c t t a a t

4800 EF-1 α intron A c a t c a a g t g c c a a g c t g g c c t a a c t t c a g t c t c c a c c c a c t c a g t g t g g g g a a a c t c c a t g t a g t t c a c g g t t c g a c c g g a t t g a a g t c a g a g g t g g g t g a g t c a c a c c c c t t t g a g g t a

4860 EF-1 α intron A c g c a t a a a a c c c c t c c c c c c a a c c t a a a g a c g a c g t a c t c c a a a a g c t c c a g a a c t a a t c g c g t a t t t t g g g g a g g g g g g t t g g a t t t c t g c t g c a t g a g g t t t t c g a g g t c t t g a t t a g

BbvCI 4920 EF-1 α intron A MKRRLRT g a g g t g c c t g g a c g g c g c c c g g t a c t c c g t g g a g t c a c a t g a a g c g a c g g c t g a g g a c g g c t c c a c g g a c c t g c c g c g g g c c a t g a g g c a c c t c a g t g t a c t t c g c t g c c g a c t c c t g c c

4980 EF-1 α intron A ERPFSFVWVTHPPALPSAAS a a a g g c c c t t t t c c t t t g t g t g g g t g a c t c a c c c g c c c g c t c t c c c g a g c g c c g c g t c c t t t t c c g g g a a a a g g a a a c a c a c c c a c t g a g t g g g c g g g c g a g a g g g c t c g c g g c g c a g g a

BstAPI 5040 EF-1 α intron A SILSPLEQGREAAIFPLTQL c c a t t t t g a g c c c c c t g g a g c a g g g c c g g g a a g c g g c c a t c t t t c c g c t c a c g c a a c t g g g g t a a a a c t c g g g g g a c c t c g t c c c g g c c c t t c g c c g g t a g a a a g g c g a g t g c g t t g a c c

5100 EF-1 α intron A VPTGPALPPRAGRYTAARGQ t g c c g a c c g g g c c a g c c t t g c c g c c c a g g g c g g g g c g a t a c a c g g c g g c g c g a g g c c a g g a c g g c t g g c c c g g t c g g a a c g g c g g g t c c c g c c c c g c t a t g t g c c g c c g c g c t c c g g t c c

5160 EF-1 α intron A APEQAGQLETTPVRFSVAAL c a c c a g a g c a g g c c g g c c a g c t t g a g a c t a c c c c c g t c c g a t t c t c g g t g g c c g c g c t c g g t g g t c t c g t c c g g c c g g t c g a a c t c t g a t g g g g g c a g g c t a a g a g c c a c c g g c g c g a g c

5220 EF-1 α intron A AGPASPNMCAGTHGPRRRPR c a g g c c c c g c c t c g c c g a a c a t g t g c g c t g g g a c g c a c g g g c c c c g t c g c c g g c c g c g g g g t c c g g g g c g g a g c g g c t t g t a c a c g c g a c c c t g c g t g c c c g g g g c a g c g g c c g g c g c c c

5280 EF-1 α intron A AQKPKYQCADPGPHLQDYLA c c c a a a a a c c g a a a t a c c a g t g t g c a g a t c c t g g c c c g c a t t t a c a a g a c t a t c t t g c c a g g g t t t t t g g c t t t a t g g t c a c a c g t c t a g g a c c g g g c g t a a a t g t t c t g a t a g a a c g g t

5340 EF-1 α intron A RKKASQHVIKNFKWLETYRK g a a a a a a a g c g t c g c a g c a c g t c a t c a a a a a t t t t a a a t g g c t a g a g a c t t a t c g a a a g c c t t t t t t t c g c a g c g t c g t g c a g t a g t t t t t a a a a t t t a c c g a t c t c t g a a t a g c t t t c g

5400 EF-1 α intron A QRDRREGATRFARGGPSAQA a g c g a g a c a g g c g c g a a g g t g c c a c c a g a t t c g c a c g c g g c g g c c c c a g c g c c c a g g c c a t c g c t c t g t c c g c g c t t c c a c g g t g g t c t a a g c g t g c g c c g c c g g g g t c g c g g g t c c g g t

StuI* 5460 EF-1 α intron A RPQLKHEAKGLLKRKARPWL g g c c t c a a c t c a a g c a c g a g g c g a a g g g g c t c c t a a a g c g c a a g g c c c g c c c c t g g c t c c c c g g a g t t g a g t t c g t g c t c c g c t t c c c c g a g g a t t t c g c g t t c c g g g c g g g g a c c g a g g

5520 EF-1 α intron A QLGIKNHVLEPGGSNSRHAR a g c t c g g g a t c a a g a a t c a c g t a c t g g a g c c a g g t g g a a g t a a t t c a a g g c a c g c a a g g g t c g a g c c c t a g t t c t t a g t g c a t g a c c t c g g t c c a c c t t c a t t a a g t t c c g t g c g t t c c c

5580 EF-1 α intron A AITRKEARPAGTTHGTYLCS c c a t a a c c c g t a a a g a g g c c a g g c c c g c g g g a a c c a c a c a c g g c a c t t a c c t g t g t t c t g g g t a t t g g g c a t t t c t c c g g t c c g g g c g c c c t t g g t g t g t g c c g t g a a t g g a c a c a a g a c

5640 GGKPVAKKNVHGDYCTYIRF g c g g c a a a c c c g t t g c g a a a a a g a a c g t t c a c g g c g a c t a c t g c a c t t a t a t a c g g t t c t c g c c g t t t g g g c a a c g c t t t t t c t t g c a a g t g c c g c t g a t g a c g t g a a t a t a t g c c a a g a

5700 SPTLGKKAEPVHDITFPVYP c c c c c a c c c t c g g g a a a a a g g c g g a g c c a g t a c a c g a c a t c a c t t t c c c a g t t t a c c c c g g g g g g t g g g a g c c c t t t t t c c g c c t c g g t c a t g t g c t g t a g t g a a a g g g t c a a a t g g g g c

SpeIAgeI 5760 APPSLGTGSIGCRTSLVKSF c g c c a c c t t c t c t a g g c a c c g g t t c a a t t g g c t g c a g g a c t a g t c t a g t t a a a a g t t t t g g c g g t g g a a g a g a t c c g t g g c c a a g t t a a c c g a c g t c c t g a t c a g a t c a a t t t t c a a a a c

5820 VTL* t t a c t t t a t a g a a g a a a t t t t g a g t t t t t g t t t t t t t t t a a t a a a t a a a t a a a c a t a a a t a a t g a a a t a t c t t c t t t a a a a c t c a a a a a c a a a a a a a a a t t a t t t a t t t a t t t g t a t t t a

5880 a a a t t g t t t g t t g a a t t t a t t a t t a g t a t g t a a g t g t a a a t a t a a t a a a a c t t a a t a t c t t t t a a c a a a c a a c t t a a a t a a t a a t c a t a c a t t c a c a t t t a t a t t a t t t t g a a t t a t a g a

5940 piggyBac right (3') inverted repeat a t t c a a a t t a a t a a a t a a a c c t c g a t a t a c a g a c c g a t a a a a c a c a t g c g t c a a t t t t a c t a a g t t t a a t t a t t t a t t t g g a g c t a t a t g t c t g g c t a t t t t g t g t a c g c a g t t a a a a t g

6000 piggyBac right (3') inverted repeat g c a t g a t t a t c t t t a a c g t a c g t c a c a a t a t g a t t a t c t t t c t a g g g t t a a t c t a g t g t t c g t a c t a a t a g a a a t t g c a t g c a g t g t t a t a c t a a t a g a a a g a t c c c a a t t a g a t c a c a a

PmeI 6060 T3 promoter G G A A A T C A C T C C C A A T T A A C G T3 t a a a c g g c c g c t t a a t t a a t c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t a t t t g c c g g c g a a t t a a t t a g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a

6120 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t

6180 -10 -35 lac promoter CAP binding site c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a

6240 CAP binding site c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a

6300 g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g

6360 G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t

6420 c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c

6480 ori a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t

6540 ori t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t

6600 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g

6660 ori t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g

6720 ori g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a

6780 ori g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c

6840 ori t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c

6900 ori g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t

6960 ori c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c

7020 ori a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a

7080 ori t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a

7140 t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c

7200 a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a

7260 285 280 *WHKILSAG AmpR *WHKILSAG c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g

AhdI 7320 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a

BsaI 7380 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g

7440 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c

7500 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c

7560 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a

7620 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c

7680 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a

7740 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g

ScaI 7800 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g

7860 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A Amp-R NQSYHIRRGLQEQGADIRSL a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t

7920 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR T A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c

7980 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g

8040 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c

8100 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a

8160 AmpR promoter c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a

8220 AmpR promoter t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g

8280 f1 ori a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g

8340 f1 ori t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g

8400 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g

8460 f1 ori t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t

8520 f1 ori c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c

8580 f1 ori A G G G A A G F1ori-R a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c

8640 f1 ori A A A G C G A A A G G A G F1ori-R a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g

8700 f1 ori a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c a g g c t g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g t c c g a c

8760 c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t

8820 C C C A G T C A C G A C G T M13/pUC Forward g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a

3ʹ 5ʹ 8858 M13 fwd T7 promoter T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C T7 t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g a c t c a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c t g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.