Addgene pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R


Map View

pBRforEco (7927 .. 7945) Amp-R (7517 .. 7536) AhdI (6967) pBR322ori-F (6215 .. 6234) PshAI (5686) HpaI (5513) SV40pA-R (5457 .. 5476) EBV-rev (5403 .. 5422) BamHI (5378) SacI (5357) Eco53kI (5355) StuI (5349) BGH-rev (5329 .. 5346) PmeI (5313) 6xHis MluI (5277) XbaI (5216) Bsu36I (4663) PflFI - Tth111I (4471) BmgBI (4470) BglII (132) AsiSI (391) TspMI - XmaI (425) SmaI (427) BbvCI (502) BsiWI (1096) SphI (1105) KasI (1388) NarI (1389) SfoI (1390) PluTI (1392) NsiI (1406) BspDI - ClaI (1708) AflII (1934) SpeI (1958) NdeI (2120) Acc65I (2579) KpnI (2583) EGFP-N (2633 .. 2654) EXFP-R (2894 .. 2913) EGFP-C (3241 .. 3262) HindIII (3307) XcmI (3435) BspQI - SapI - SgrDI (3537) NotI (3751) pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R 7953 bp

Sequence View

5ʹ 3ʹ 60 Ac5 promoter c t t c a a g g a a t t a a t t c t a t a t t c t a a a a a c a c a a a t g a t a c t t c t a a a a a a a a t c a t g a g a a g t t c c t t a a t t a a g a t a t a a g a t t t t t g t g t t t a c t a t g a a g a t t t t t t t t a g t a c t

120 Ac5 promoter a t g g c a t c a a c t c t g a a t c a a a t c t t t g c a g a t g c a c c t a c t t c t c a t t t c c a c t g t c a c t a c c g t a g t t g a g a c t t a g t t t a g a a a c g t c t a c g t g g a t g a a g a g t a a a g g t g a c a g t g

BglII 180 Ac5 promoter a t c a t t t t t c c a g a t c t c g c t g c c t g t t a t g t g g c c c a c a a a c c a a g a c a c g t t t t a t g g t a g t a a a a a g g t c t a g a g c g a c g g a c a a t a c a c c g g g t g t t t g g t t c t g t g c a a a a t a c c

240 Ac5 promoter c c a t t a a a g c t g g c t g a t c g t c g c c a a a c a c c a a a t a c a t a t c a a t a t g t a c a t t c g a g a g g t a a t t t c g a c c g a c t a g c a g c g g t t t g t g g t t t a t g t a t a g t t a t a c a t g t a a g c t c t

300 Ac5 promoter a a g a a g c g a t c a a a g a a g c g t c t t c g g g c g a g t a g g a g a a t g c g g a g g a g a a g g a g a a c g t t c t t c g c t a g t t t c t t c g c a g a a g c c c g c t c a t c c t c t t a c g c c t c c t c t t c c t c t t g c

360 Ac5 promoter a g c t g a t c t a g t a t c t c t c c a c a a t c c a a t g c c a a c t g a c c a a c t g g c c a t a t t c g g a g c t c g a c t a g a t c a t a g a g a g g t g t t a g g t t a c g g t t g a c t g g t t g a c c g g t a t a a g c c t c g

AsiSI 420 Ac5 promoter a a t t t g a a g c c a a t t t c c a t c g c c t g g c g a t c g c t c c a t t c t t g g c t a t a t g t t t t t c a c t t a a a c t t c g g t t a a a g g t a g c g g a c c g c t a g c g a g g t a a g a a c c g a t a t a c a a a a a g t g

TspMIXmaISmaI 480 Ac5 promoter c g t t c c c g g g g c c a t t t t c a a a g a c t c g t c g g t a a g a t a a g a t t g t g t c a c t c g c t g t c t g c a a g g g c c c c g g t a a a a g t t t c t g a g c a g c c a t t c t a t t c t a a c a c a g t g a g c g a c a g a

BbvCI 540 Ac5 promoter c t c t t c a t t t g t c g a a g a a t g c t g a g g a a t t t c g c g a t g a c g t c g g c g a g t a t t t t g a a g g a g a a g t a a a c a g c t t c t t a c g a c t c c t t a a a g c g c t a c t g c a g c c g c t c a t a a a a c t t c

600 Ac5 promoter a a t g a g a a t a a t t t g t a t t t a t a c g a a a a t c a g t t a g t g g a a t t t t c t a c a a a a a c a t g t t t a c t c t t a t t a a a c a t a a a t a t g c t t t t a g t c a a t c a c c t t a a a a g a t g t t t t t g t a c a

660 Ac5 promoter t a t c t a t a g a t a a t t t t g t t g c a a a a t a t g t t g a c t a t g a c a a a g a t t g t a t g t a t a t a c a t a g a t a t c t a t t a a a a c a a c g t t t t a t a c a a c t g a t a c t g t t t c t a a c a t a c a t a t a t g

720 Ac5 promoter c t t t a a t g t a t t c t c a t t t t c t t a t g t a t t t a t a a t g g c a a t g a t g a t a c t g a t g a t a t t g a a a t t a c a t a a g a g t a a a a g a a t a c a t a a a t a t t a c c g t t a c t a c t a t g a c t a c t a t a a

780 Ac5 promoter MMPDHRLISASFAERSAC t t a a g a t g a t g c c a g a c c a c a g g c t g a t t t c t g c g t c t t t t g c c g a a c g c a g t g c a t g t g a a t t c t a c t a c g g t c t g g t g t c c g a c t a a a g a c g c a g a a a a c g g c t t g c g t c a c g t a c a c

840 Ac5 promoter AVVVFWNSFNFRTVRFDFSF c g g t t g t t g t t t t t t g g a a t a g t t t c a a t t t t c g g a c t g t c c g c t t t g a t t t c a g t t t c t g c c a a c a a c a a a a a a c c t t a t c a a a g t t a a a a g c c t g a c a g g c g a a a c t a a a g t c a a a g a

900 Ac5 promoter LAYSKSKVKPKKRDGNTKCG t g g c t t a t t c a a a a a g c a a a g t a a a g c c a a a a a a g c g a g a t g g c a a t a c c a a a t g c g g c a a c c g a a t a a g t t t t t c g t t t c a t t t c g g t t t t t t c g c t c t a c c g t t a t g g t t t a c g c c g t

960 Ac5 promoter KTVVEGKGCGAAEGRVGRGV a a a c g g t a g t g g a a g g a a a g g g g t g c g g g g c a g c g g a a g g a a g g g t g g g g c g g g g c g t g g t t t g c c a t c a c c t t c c t t t c c c c a c g c c c c g t c g c c t t c c t t c c c a c c c c g c c c c g c a c c

1020 Ac5 promoter AGSVAGRDVTDVGATPLTMC c g g g g t c t g t g g c t g g g c g c g a c g t c a c c g a c g t t g g a g c c a c t c c t t t g a c c a t g t g t g g c c c c a g a c a c c g a c c c g c g c t g c a g t g g c t g c a a c c t c g g t g a g g a a a c t g g t a c a c a c

1080 Ac5 promoter ACVYYSCLATRRLFFSFYLA c g t g t g t g t a t t a t t c g t g t c t c g c c a c t c g c c g g t t g t t t t t t t c t t t t t a t c t c g c t c g c a c a c a c a t a a t a a g c a c a g a g c g g t g a g c g g c c a a c a a a a a a a g a a a a a t a g a g c g a g

SphIBsiWI 1140 Ac5 promoter LSSAISYACSTHRMLPCPLC t c t c t a g c g c c a t c t c g t a c g c a t g c t c a a c g c a c c g c a t g t t g c c g t g t c c t t t a t g c g a g a g a t c g c g g t a g a g c a t g c g t a c g a g t t g c g t g g c g t a c a a c g g c a c a g g a a a t a c g c

1200 Ac5 promoter VILARNRQLFKQRLVNENAK t c a t t t t g g c t c g a a a t a g g c a a t t a t t t a a a c a a a g a t t a g t c a a c g a a a a c g c t a a a a a g t a a a a c c g a g c t t t a t c c g t t a a t a a a t t t g t t t c t a a t c a g t t g c t t t t g c g a t t t t

1260 Ac5 promoter INKSTIWLLIAMCVQPTIAT t a a a t a a g t c t a c a a t a t g g t t a c t t a t t g c c a t g t g t g t g c a g c c a a c g a t a g c a a c a a a t t t a t t c a g a t g t t a t a c c a a t g a a t a a c g g t a c a c a c a c g t c g g t t g c t a t c g t t g t t

1320 Ac5 promoter KATTQWLSLFHFLFASACEQ a a g c a a c a a c a c a g t g g c t t t c c c t c t t t c a c t t t t t g t t t g c a a g c g c g t g c g a g c a a g t t c g t t g t t g t g t c a c c g a a a g g g a g a a a g t g a a a a a c a a a c g t t c g c g c a c g c t c g t t c

1380 Ac5 promoter DGTTGKRNYADKEQTKFWPK a c g g c a c g a c c g g c a a a c g c a a t t a c g c t g a c a a a g a g c a g a c g a a g t t t t g g c c g a a a a t g c c g t g c t g g c c g t t t g c g t t a a t g c g a c t g t t t c t c g t c t g c t t c a a a a c c g g c t t t t

NsiIKasINarISfoIPluTI 1440 Ac5 promoter NIKAPDTNAFAITIAIFNIV a c a t c a a g g c g c c t g a t a c g a a t g c a t t t g c a a t a a c a a t t g c g a t a t t t a a t a t t g t t t t g t a g t t c c g c g g a c t a t g c t t a c g t a a a c g t t a t t g t t a a c g c t a t a a a t t a t a a c a a a

1500 Ac5 promoter YEAV* a t g a a g c t g t t t g a c t t c a a a a c a c a c a a a a a a a a a a a t a a a a c a a a t t a t t t g a a a g a g t a c t t c g a c a a a c t g a a g t t t t g t g t g t t t t t t t t t t t a t t t t g t t t a a t a a a c t t t c t c

1560 Ac5 promoter a a t t a g g a a t c g g a c a g c t t a t c g t t a c g g g c t a a c a g c a c a c c g a g a c g a a a t a g c t t a t t a a t c c t t a g c c t g t c g a a t a g c a a t g c c c g a t t g t c g t g t g g c t c t g c t t t a t c g a a t

1620 Ac5 promoter c c t g a c g t c a c a g c c t c t g g a a g a a c t g c c g c c a a g c a g a g a g a g a g a g a a a a a g a g g g a g g a c t g c a g t g t c g g a g a c c t t c t t g a c g g c g g t t c g t c t c t c t c t c t c t t t t t c t c c c t

1680 Ac5 promoter g a g c a g c t t a g a c c g c a t g t g c t t g t g t g t g a g g c g t c t c t c t c t t c g t c t c c t g t t t g c c t c g t c g a a t c t g g c g t a c a c g a a c a c a c a c t c c g c a g a g a g a g a a g c a g a g g a c a a a c g

BspDIClaI 1740 Ac5 promoter g c a a a c g c a t a g a c t g c a c t g a g a a a a t c g a t t a c c t a t t t t t t a t g a a t g a a t a t t t g c c g t t t g c g t a t c t g a c g t g a c t c t t t t a g c t a a t g g a t a a a a a a t a c t t a c t t a t a a a c g

1800 Ac5 promoter a c t a t t a c t a t t c a a a a c t a t t a a g a t a g c a a t c a c a t t c a a t a g c c a a a t a c t a t a c c a t g a t a a t g a t a a g t t t t g a t a a t t c t a t c g t t a g t g t a a g t t a t c g g t t t a t g a t a t g g t

1860 Ac5 promoter c c t g a g c g a t g c a a c g a a a t g a t c a a t t t g a g c a a a a a t g c t g c a t a t t t a g g a c g g c a t g g a c t c g c t a c g t t g c t t t a c t a g t t a a a c t c g t t t t t a c g a c g t a t a a a t c c t g c c g t a

1920 Ac5 promoter c a t t a t a g a a a t g c t t c t t g c t g t g t a c t t t t c t c t c g t c t g g c a g c t g t t t c g c c g t t a g t a a t a t c t t t a c g a a g a a c g a c a c a t g a a a a g a g a g c a g a c c g t c g a c a a a g c g g c a a t

SpeIAflII 1980 Ac5 promoter *THKRRSTIGRSSS t t g t t a a a a c c g g c t t a a g t t a g g t g t g t t t t c t a c g a c t a g t g a t g c c c c t a c t a g a a g a a c a a t t t t g g c c g a a t t c a a t c c a c a c a a a a g a t g c t g a t c a c t a c g g g g a t g a t c t t c

2040 Ac5 promoter THQVFTGSYGIQLASLLLRL a t g t g t g t t g c a c a a a t g t c c c t g a a t a a c c a a t t t g a a g t g c a g a t a g c a g t a a a c g t a t a c a c a c a a c g t g t t t a c a g g g a c t t a t t g g t t a a a c t t c a c g t c t a t c g t c a t t t g c a t

2100 Ac5 promoter SIHINNLQLTKIDSSMVLLG a g c t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c g c t g g a c a t t a c t a a t a a a c t c g a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a g c g a c c t g t a a t g a t t a t t t g

NdeI 2160 Ac5 promoter VIFVHVYTHKPMCHTTPSFI c c a c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c a a t g a g t a g t t g g g g a a a a a a g g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g t t a c t c a t c a a c c c c t t t t t t

2220 Ac5 promoter HLLVTVMVAYLYGASTDFIL t g t g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c c a g c t g a a g t a t c g a a t a t g a a c a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g g t c g a c t t c a t a g c t t a t a c t

2280 Ac5 promoter LGWISDCAAVSLVWPLVSKM g t a a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a c c c a t g g a a g t a c a c t c t t c a c a t t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t g g g t a c c t t c a t g t g a g a a g t

2340 Ac5 promoter AIYLVCVLVFVWNRLFNETF t g g c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t t g c g g a g g a a a t t c t c c g t a a a c c g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a a c g c c t c c t t t a a g a g g c a t t

2400 Ac5 promoter SFGIPSCNMGMYPLLKSRSK a t g a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g g t a a a a g t t t t g a a c g c g a c t t a c t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c c a t t t t c a a a a c t t g c g c t g a

2460 Ac5 promoter LASLM t g a g a g c g g a g a g c a t t g c g g c t g a t a a g g t t t t a g c g c t a a g c g g g c t t t a t a a a a c g g a c t c t c g c c t c t c g t a a c g c c g a c t a t t c c a a a a t c g c g a t t c g c c c g a a a t a t t t t g c c

2520 Ac5 promoter g c t g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c t t g t g c t g t g t g g a t a c t c c t c g a c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g a a c a c g a c a c a c c t a t g a g g a

Acc65I 2580 Ac5 promoter c c c g a c a c a a a g c c g c t c c a t c a g c c a g c a g t c g t c t a a t c c a g a g a c c c c g g a t c g g g g g g g c t g t g t t t c g g c g a g g t a g t c g g t c g t c a g c a g a t t a g g t c t c t g g g g c c t a g c c c c

KpnI 2640 1 M 1a V 5 10 15 SKGEELFTGVVPILVE EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPILVE EGFP G A C C A G C T EGFP-N MVSKGEELFTGVVPILVE t a c c a a c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a a t g g t t g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t

2700 20 25 30 35 LDGDVNGHKFSVSGEGEGDA EGFP 20 25 30 35 LDGDVNGHKFSVSGEGEGDA EGFP C G A C C T G C C G C T G C EGFP-N LDGDVNGHKFSVSGEGEGDA g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g

2760 40 45 50 55 TYGKLTLKFICTTGKLPVPW EGFP 40 45 50 55 TYGKLTLKFICTTGKLPVPW EGFP TYGKLTLKFICTTGKLPVPW c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c

2820 60 65 70 75 PTLVTTLTYGVQCFSRYPDH EGFP 60 65 70 75 PTLVTTLTYGVQCFSRYPDH EGFP PTLVTTLTYGVQCFSRYPDH g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t

2880 80 85 90 95 MKQHDFFKSAMPEGYVQERT EGFP 80 85 90 95 MKQHDFFKSAMPEGYVQERT EGFP MKQHDFFKSAMPEGYVQERT c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g

2940 100 105 110 115 IFFKDDGNYKTRAEVKFEGD EGFP 100 105 110 115 IFFKDDGNYKTRAEVKFEGD EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R IFFKDDGNYKTRAEVKFEGD c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t

3000 120 125 130 135 TLVNRIELKGIDFKEDGNIL EGFP 120 125 130 135 TLVNRIELKGIDFKEDGNIL EGFP TLVNRIELKGIDFKEDGNIL c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a

3060 140 145 150 155 GHKLEYNYNSHNVYIMADKQ EGFP 140 145 150 155 GHKLEYNYNSHNVYIMADKQ EGFP GHKLEYNYNSHNVYIMADKQ g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t

3120 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ EGFP 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ EGFP KNGIKVNFKIRHNIEDGSVQ g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t

3180 180 185 190 195 LADHYQQNTPIGDGPVLLPD EGFP 180 185 190 195 LADHYQQNTPIGDGPVLLPD EGFP LADHYQQNTPIGDGPVLLPD g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t

3240 200 205 210 215 NHYLSTQSALSKDPNEKRDH EGFP 200 205 210 215 NHYLSTQSALSKDPNEKRDH EGFP NHYLSTQSALSKDPNEKRDH c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t

3300 220 225 230 235 MVLLEFVTAAGITLGMDELY EGFP 220 225 230 235 MVLLEFVTAAGITLGMDELY EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C MVLLEFVTAAGITLGMDELY c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t

HindIII 3360 K (in frame with EGFP) PSLMDDYSMWTDENDEDAK EGFP K (in frame with EGFP) PSLMDDYSMWTDENDEDAK EGFP KPSLMDDYSMWTDENDEDAK c a a g c c a a g c t t a a t g g a t g a t t a t t c a a t g t g g a c t g a t g a g a a t g a t g a a g a c g c g a a g t t c g g t t c g a a t t a c c t a c t a a t a a g t t a c a c c t g a c t a c t c t t a c t a c t t c t g c g c t t

3420 (in frame with EGFP) KKKSLRDKGLSGWGDADVSN (in frame with EGFP) KKKSLRDKGLSGWGDADVSN KKKSLRDKGLSGWGDADVSN a a a g a a a a a a t c g t t g a g a g a t a a g g g a t t g a g t g g a t g g g g t g a t g c t g a t g t a t c a a a t t t c t t t t t t a g c a a c t c t c t a t t c c c t a a c t c a c c t a c c c c a c t a c g a c t a c a t a g t t t

XcmI 3480 (in frame with EGFP) SKPIRRWIVPEGQEEDFETA (in frame with EGFP) SKPIRRWIVPEGQEEDFETA SKPIRRWIVPEGQEEDFETA c a g t a a a c c a a t t c g c c g t t g g a t t g t g c c a g a a g g c c a a g a a g a a g a c t t t g a a a c g g c g t c a t t t g g t t a a g c g g c a a c c t a a c a c g g t c t t c c g g t t c t t c t t c t g a a a c t t t g c c g

SgrDIBspQISapI 3540 (in frame with EGFP) MERCPSHLKKKALNEEALRR (in frame with EGFP) MERCPSHLKKKALNEEALRR MERCPSHLKKKALNEEALRR a a t g g a a c g a t g t c c a a g t c a t t t g a a a a a g a a a g c a t t g a a t g a a g a a g c t c t t c g t c g t t a c c t t g c t a c a g g t t c a g t a a a c t t t t t c t t t c g t a a c t t a c t t c t t c g a g a a g c a g c

3600 (in frame with EGFP) RLTSENPQIVAQAQQQAEEE (in frame with EGFP) RLTSENPQIVAQAQQQAEEE RLTSENPQIVAQAQQQAEEE a c g t t t a a c a t c t g a a a a t c c a c a a a t a g t g g c a c a a g c t c a a c a a c a a g c c g a a g a a g a t g c a a a t t g t a g a c t t t t a g g t g t t t a t c a c c g t g t t c g a g t t g t t g t t c g g c t t c t t c t

3660 (in frame with EGFP) ANAMMRIGRRPIVPSGWGDI (in frame with EGFP) ANAMMRIGRRPIVPSGWGDI ANAMMRIGRRPIVPSGWGDI g g c g a a t g c t a t g a t g a g g a t t g g a c g a a g a c c c a t t g t t c c c a g t g g a t g g g g t g a t a t c c g c t t a c g a t a c t a c t c c t a a c c t g c t t c t g g g t a a c a a g g g t c a c c t a c c c c a c t a t a

3720 (in frame with EGFP) PSEISTDKSEFDQSSSRGWD (in frame with EGFP) PSEISTDKSEFDQSSSRGWD PSEISTDKSEFDQSSSRGWD t c c a t c t g a a a t t a g t a c t g a c a a g t c a g a a t t c g a t c a a a g c t c g t c a c g t g g t t g g g a a g g t a g a c t t t a a t c a t g a c t g t t c a g t c t t a a g c t a g t t t c g a g c a g t g c a c c a a c c c t

NotI 3780 (in frame with EGFP) EGSISGGGSCGRAQALLVKQ (in frame with EGFP) EGSISGGGSCGRAQALLVKQ EGSISGGGSCGRAQALLVKQ t g a a g g a a g t a t a a g c g g t g g t g g a a g t t g c g g c c g c g c c c a g g c c c t g c t c g t g a a g c a a c t t c c t t c a t a t t c g c c a c c a c c t t c a a c g c c g g c g c g g g t c c g g g a c g a g c a c t t c g t

3840 (in frame with EGFP) PPPPPPPPHLSLHPSAGKSA (in frame with EGFP) PPPPPPPPHLSLHPSAGKSA PPPPPPPPHLSLHPSAGKSA g c c a c c a c c g c c a c c g c c c c c g c c g c a c c t g t c t c t g c a c c c c t c t g c a g g c a a a t c g g c c g g t g g t g g c g g t g g c g g g g g c g g c g t g g a c a g a g a c g t g g g g a g a c g t c c g t t t a g c c g

3900 (in frame with EGFP) MDSFPSHPQTPGLPDLQTKE (in frame with EGFP) MDSFPSHPQTPGLPDLQTKE MDSFPSHPQTPGLPDLQTKE c a t g g a c a g c t t c c c c t c g c a c c c a c a g a c t c c c g g c c t a c c t g a c c t g c a g a c c a a a g a g t a c c t g t c g a a g g g g a g c g t g g g t g t c t g a g g g c c g g a t g g a c t g g a c g t c t g g t t t c t

3960 (in frame with EGFP) QQSSPNTFAPYPLAGLNPNM (in frame with EGFP) QQSSPNTFAPYPLAGLNPNM QQSSPNTFAPYPLAGLNPNM g c a g c a g t c t t c a c c c a a c a c c t t t g c t c c t t a c c c t c t c g c t g g a c t g a a c c c a a a c a t c g t c g t c a g a a g t g g g t t g t g g a a a c g a g g a a t g g g a g a g c g a c c t g a c t t g g g t t t g t a

4020 (in frame with EGFP) NVNSMDMTGGLSVKDPSQSQ (in frame with EGFP) NVNSMDMTGGLSVKDPSQSQ NVNSMDMTGGLSVKDPSQSQ g a a t g t c a a c a g c a t g g a c a t g a c c g g t g g c t t g t c g g t g a a g g a c c c a t c c c a g t c c c a c t t a c a g t t g t c g t a c c t g t a c t g g c c a c c g a a c a g c c a c t t c c t g g g t a g g g t c a g g g t

4080 (in frame with EGFP) SRLPQWTHPNSMDNLPSAAS (in frame with EGFP) SRLPQWTHPNSMDNLPSAAS SRLPQWTHPNSMDNLPSAAS g t c a c g c c t c c c c c a g t g g a c g c a c c c c a a c t c c a t g g a t a a c t t g c c c a g t g c c g c t t c c a g t g c g g a g g g g g t c a c c t g c g t g g g g t t g a g g t a c c t a t t g a a c g g g t c a c g g c g a a g

4140 (in frame with EGFP) PLEQNPSKHGAIPGGLSIGP (in frame with EGFP) PLEQNPSKHGAIPGGLSIGP PLEQNPSKHGAIPGGLSIGP c c c c c t g g a g c a g a a c c c t a g c a a g c a t g g t g c t a t c c c t g g a g g t c t a a g c a t t g g g c c g g g g g a c c t c g t c t t g g g a t c g t t c g t a c c a c g a t a g g g a c c t c c a g a t t c g t a a c c c g g

4200 (in frame with EGFP) PGKSSIDDSYGRYDLIQNSE (in frame with EGFP) PGKSSIDDSYGRYDLIQNSE PGKSSIDDSYGRYDLIQNSE t c c a g g t a a g t c c t c c a t t g a t g a c t c c t a t g g c c g g t a c g a t t t a a t c c a g a a c a g t g a a g g t c c a t t c a g g a g g t a a c t a c t g a g g a t a c c g g c c a t g c t a a a t t a g g t c t t g t c a c t

4260 (in frame with EGFP) SPASPPVAVPHSWSRAKSDS (in frame with EGFP) SPASPPVAVPHSWSRAKSDS SPASPPVAVPHSWSRAKSDS g t c a c c a g c c a g t c c t c c c g t a g c t g t t c c c c a t a g c t g g t c a c g t g c c a a a t c t g a c a g c a g t g g t c g g t c a g g a g g g c a t c g a c a a g g g g t a t c g a c c a g t g c a c g g t t t a g a c t g t c

4320 (in frame with EGFP) DKISNGSSINWPPEFHPGVP (in frame with EGFP) DKISNGSSINWPPEFHPGVP DKISNGSSINWPPEFHPGVP MALASTGPQNSIREF t g a t a a a a t c t c a a a t g g c t c t a g c a t c a a c t g g c c c c c a g a a t t c c a t c c g g g a g t t c c a c t a t t t t a g a g t t t a c c g a g a t c g t a g t t g a c c g g g g g t c t t a a g g t a g g c c c t c a a g g

4380 (in frame with EGFP) WKGLQNIDPENDPDVTPGSV (in frame with EGFP) WKGLQNIDPENDPDVTPGSV WKGLQNIDPENDPDVTPGSV HGKDCRILTLRMTLTSLLAV a t g g a a a g g a c t g c a g a a t a t t g a c c c t g a g a a t g a c c c t g a c g t c a c t c c t g g c a g t g t t a c c t t t c c t g a c g t c t t a t a a c t g g g a c t c t t a c t g g g a c t g c a g t g a g g a c c g t c a c a

4440 (in frame with EGFP) PTGPTINTTIQDVNRYLLKS (in frame with EGFP) PTGPTINTTIQDVNRYLLKS PTGPTINTTIQDVNRYLLKS SPLGLPSTPPSRMSTATSSR c c c c a c t g g g c c t a c c a t c a a c a c c a c c a t c c a g g a t g t c a a c c g c t a c c t c c t c a a g a g g g g g t g a c c c g g a t g g t a g t t g t g g t g g t a g g t c c t a c a g t t g g c g a t g g a g g a g t t c t c

BmgBITth111IPflFI 4500 (in frame with EGFP) GGKLSDIKSTWSSGPTSHTQ (in frame with EGFP) GGKLSDIKSTWSSGPTSHTQ GGKLSDIKSTWSSGPTSHTQ VEVNCQTSNRRGPLALPPTR t g g a g g t a a a c t g t c a g a c a t c a a a t c g a c g t g g t c c t c t g g c c c t a c c t c c c a c a c g c a a c c t c c a t t t g a c a g t c t g t a g t t t a g c t g c a c c a g g a g a c c g g g a t g g a g g g t g t g c g t

4560 (in frame with EGFP) ASLSHELWKVPRNSTAPTRP (in frame with EGFP) ASLSHELWKVPRNSTAPTRP ASLSHELWKVPRNSTAPTRP KPLCLMNYGRCPETVLHPRG a g c c t c t c t g t c t c a t g a a c t a t g g a a g g t g c c c a g a a a c a g t a c t g c a c c c a c g a g g c c t c g g a g a g a c a g a g t a c t t g a t a c c t t c c a c g g g t c t t t g t c a t g a c g t g g g t g c t c c g g

4620 (in frame with EGFP) PPAATNPKPSSTWGASPLGW (in frame with EGFP) PPAATNPKPSSTWGASPLGW PPAATNPKPSSTWGASPLGW HLQQQPIPSPPPPGVPAPSA a c c t c c a g c a g c a a c c a a t c c c a a g c c c t c c t c c a c c t g g g g t g c c a g c c c c c t c g g c t g t g g a g g t c g t c g t t g g t t a g g g t t c g g g a g g a g g t g g a c c c c a c g g t c g g g g g a g c c g a c

Bsu36I 4680 (in frame with EGFP) TSSYSSGSAWSTDTSGRTSS (in frame with EGFP) TSSYSSGSAWSTDTSGRTSS TSSYSSGSAWSTDTSGRTSS GPAPTPRVLPGAPTPQEEPA g a c c a g c t c c t a c t c c t c g g g t t c t g c c t g g a g c a c c g a c a c c t c a g g a a g a a c c a g c a g c t g g t c g a g g a t g a g g a g c c c a a g a c g g a c c t c g t g g c t g t g g a g t c c t t c t t g g t c g t c

4740 (in frame with EGFP) WLVLRNLTPQIDGSTLRTLC (in frame with EGFP) WLVLRNLTPQIDGSTLRTLC WLVLRNLTPQIDGSTLRTLC AGSFFETSLPRLMVLHCGHC c t g g c t c g t t c t t c g a a a c c t c a c t c c c c a g a t t g a t g g t t c t a c a c t g c g g a c a t t g t g g a c c g a g c a a g a a g c t t t g g a g t g a g g g g t c t a a c t a c c a a g a t g t g a c g c c t g t a a c a c

4800 (in frame with EGFP) LQHGPLITFHLNLTQGNAVV (in frame with EGFP) LQHGPLITFHLNLTQGNAVV LQHGPLITFHLNLTQGNAVV VCNMGLLSHST* t t t g c a a c a t g g g c c t c t t a t c a c a t t c c a c c t g a a t c t g a c t c a a g g c a a t g c t g t g g t a a a c g t t g t a c c c g g a g a a t a g t g t a a g g t g g a c t t a g a c t g a g t t c c g t t a c g a c a c c a

4860 (in frame with EGFP) RYSSKEEAAKAQKSLHMCVL (in frame with EGFP) RYSSKEEAAKAQKSLHMCVL RYSSKEEAAKAQKSLHMCVL c c g g t a c a g c t c c a a g g a g g a g g c t g c c a a g g c c c a g a a g t c t c t g c a c a t g t g c g t c c t g g c c a t g t c g a g g t t c c t c c t c c g a c g g t t c c g g g t c t t c a g a g a c g t g t a c a c g c a g g a

4920 (in frame with EGFP) GNTTILAEFAGEEEVNRFLA (in frame with EGFP) GNTTILAEFAGEEEVNRFLA GNTTILAEFAGEEEVNRFLA g g g a a a c a c t a c c a t c c t g g c c g a g t t c g c t g g t g a a g a a g a a g t g a a t c g c t t c t t a g c c c c t t t g t g a t g g t a g g a c c g g c t c a a g c g a c c a c t t c t t c t t c a c t t a g c g a a g a a t c g

4980 (in frame with EGFP) QGQALPPTSSWQSSSASSQP (in frame with EGFP) QGQALPPTSSWQSSSASSQP QGQALPPTSSWQSSSASSQP c c a a g g c c a g g c g c t g c c a c c c a c t t c c a g c t g g c a g t c c a g c a g c g c g t c c a g c c a g c c g g t t c c g g t c c g c g a c g g t g g g t g a a g g t c g a c c g t c a g g t c g t c g c g c a g g t c g g t c g g

5040 (in frame with EGFP) RLSAAGSSHGLVRSDAGHWN (in frame with EGFP) RLSAAGSSHGLVRSDAGHWN RLSAAGSSHGLVRSDAGHWN g c g g c t c a g c g c a g c g g g c a g c t c c c a t g g c c t g g t a c g c a g c g a c g c t g g c c a c t g g a a c g c c g a g t c g c g t c g c c c g t c g a g g g t a c c g g a c c a t g c g t c g c t g c g a c c g g t g a c c t t

5100 (in frame with EGFP) APCLGGKGSSELLWGGVPQY (in frame with EGFP) APCLGGKGSSELLWGGVPQY APCLGGKGSSELLWGGVPQY c g c c c c g t g c c t g g g t g g c a a g g g g a g c a g t g a g c t g c t g t g g g g c g g g g t g c c c c a g t a g c g g g g c a c g g a c c c a c c g t t c c c c t c g t c a c t c g a c g a c a c c c c g c c c c a c g g g g t c a t

5160 (in frame with EGFP) SSSLWGPPSADDSRVIGSPT (in frame with EGFP) SSSLWGPPSADDSRVIGSPT SSSLWGPPSADDSRVIGSPT c t c c a g c a g c c t g t g g g g c c c g c c c a g c g c c g a c g a c a g c a g g g t g a t a g g c a g c c c c a c g a g g t c g t c g g a c a c c c c g g g c g g g t c g c g g c t g c t g t c g t c c c a c t a t c c g t c g g g g t g

XbaI 5220 (in frame with EGFP) PLTTLLPGDLLSGESL* (in frame with EGFP) PLTTLLPGDLLSGESL* PLTTLLPGDLLSGESL* g c c g c t a a c c a c c c t g c t g c c t g g g g a c c t g c t c a g c g g g g a g t c c c t g t a g t a a t c t a g c g g c g a t t g g t g g g a c g a c g g a c c c c t g g a c g a g t c g c c c c t c a g g g a c a t c a t t a g a t c

MluI 5280 1 5 10 GKPIPNPLLGLDST V5 tag a g g g c c c g c g g t t c g a a g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g c t c c c g g g c g c c a a g c t t c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c g

PmeI 5340 RTG 1 5 HHHHHH * 6xHis G G A G C T G A C A C G BGH-rev g t a c c g g t c a t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c a t g g c c a g t a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g

BamHIStuIEco53kISacI 5400 SV40 poly(A) signal G A A G A T BGH-rev c t t c t a a g g c c t g a g c t c g c t g a t c a g c c t c g a t c g a g g a t c c a g a c a t g a t a a g a t a c a g a a g a t t c c g g a c t c g a g c g a c t a g t c g g a g c t a g c t c c t a g g t c t g t a c t a t t c t a t g t

5460 SV40 poly(A) signal G A A A SV40pA-R C T A C T C A A A C C T G T T T G G T G EBV-rev t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t

HpaI 5520 SV40 poly(A) signal T T T G T G A T G C T A T T G C SV40pA-R t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t

5580 *TEPEPPPTPPKKFC a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t

5640 MISRPMPLR TFGRCIHYPQNHDTSRHGQS a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c a g t c g a c c g a t g c c c t t g a g a t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g t c a g c t g g c t a c g g g a a c t c t

PshAI 5700 AFNPVSSFRWARGMTIVAAL GEVWDAGEPPRPAHSDDGCK g c c t t c a a c c c a g t c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t a t c g t c g c c g c a c t t c g g a a g t t g g g t c a g t c g a g g a a g g c c a c c c g c g c c c c g t a c t g a t a g c a g c g g c g t g a a

5760 MTVFFIMQLVGQVPAALWVI HSDEKDHLEYSLHRCREPDN a t g a c t g t c t t c t t t a t c a t g c a a c t c g t a g g a c a g g t g c c g g c a g c g c t c t g g g t c a t t t a c t g a c a g a a g a a a t a g t a c g t t g a g c a t c c t g t c c a c g g c c g t c g c g a g a c c c a g t a a

5820 FGEDRFRWSATMIGLSLAVF EALVAKAPARRHDAQRKRYE t t c g g c g a g g a c c g c t t t c g c t g g a g c g c g a c g a t g a t c g g c c t g t c g c t t g c g g t a t t c a a g c c g c t c c t g g c g a a a g c g a c c t c g c g c t g c t a c t a g c c g g a c a g c g a a c g c c a t a a g

5880 GILHALAQAFVTGPATKRFG SDQVGESLGEDSTGGGFTEA g g a a t c t t g c a c g c c c t c g c t c a a g c c t t c g t c a c t g g t c c c g c c a c c a a a c g t t t c g g c c c t t a g a a c g t g c g g g a g c g a g t t c g g a a g c a g t g a c c a g g g c g g t g g t t t g c a a a g c c g

5940 EKQAIIAGMAADALGYVLLA LLLGNDGAHRGVRQAVDQQR g a g a a g c a g g c c a t t a t c g c c g g c a t g g c g g c c g a c g c g c t g g g c t a c g t c t t g c t g g c g c t c t t c g t c c g g t a a t a g c g g c c g t a c c g c c g g c t g c g c g a c c c g a t g c a g a a c g a c c g c

6000 FATRGWMAFPIMILLASGGI ERRSAPHGEGNHNKESGAAD t t c g c g a c g c g a g g c t g g a t g g c c t t c c c c a t t a t g a t t c t t c t c g c t t c c g g c g g c a t c a a g c g c t g c g c t c c g a c c t a c c g g a a g g g g t a a t a c t a a g a a g a g c g a a g g c c g c c g t a g

6060 GMPALQAMLSRQVDDDHQGQ PHGRQLGHQGPLYIVVMLSL g g g a t g c c c g c g t t g c a g g c c a t g c t g t c c a g g c a g g t a g a t g a c g a c c a t c a g g g a c a g c c c t a c g g g c g c a a c g t c c g g t a c g a c a g g t c c g t c c a t c t a c t g c t g g t a g t c c c t g t c

6120 LQGSLAALTSQQKARNRKKA KLSRERSKGALLLGPVTFLG c t t c a a g g a t c g c t c g c g g c t c t t a c c a g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g a a g t t c c t a g c g a g c g c c g a g a a t g g t c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g

6180 ori ALLAFFHRLRPPDEHHKNRR RQQRKEMPEAGRVLM g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g

6240 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F SSQRWRNPTGL* t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t

6300 ori a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a

6360 ori c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c t c a c g c t g t a g g t a t c t c a g t t c g g t g g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t c c a t a g a g t c a a g c c a c

6420 ori t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g

6480 ori g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c

6540 ori g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g

6600 ori t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c

6660 ori c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g

6720 ori g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a

6780 ori c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a

6840 t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t

6900 *W AmpR *W a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t

6960 285 280 275 270 HKILSAGIEAIQRNREDMTA AmpR HKILSAGIEAIQRNREDMTA t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g

AhdI 7020 265 260 255 250 QSGTTYIVVIRSPKGDPGLA AmpR QSGTTYIVVIRSPKGDPGLA t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a

7080 245 240 235 230 AIIGRSGREGAGSKDAIFWG AmpR AIIGRSGREGAGSKDAIFWG g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t

7140 225 220 215 210 APLASRLLPGAVKDAEMWDI AmpR APLASRLLPGAVKDAEMWDI g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a

7200 205 200 195 190 LQQRSALTLLEGTLLKRLTT AmpR LQQRSALTLLEGTLLKRLTT a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a

7260 185 180 175 170 AMAAPMTTDREDNPIAENLE AmpR AMAAPMTTDREDNPIAENLE g c c a t t g c t g c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c c g g t a a c g a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g

7320 165 160 155 150 PEWRDLRTVHDGMNHLFATL AmpR PEWRDLRTVHDGMNHLFATL g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g

7380 145 140 135 130 EKPGGITTLLLNAATNDSMT AmpR EKPGGITTLLLNAATNDSMT t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a

7440 125 120 115 110 IAASCLERVTMGDTLHKETV AmpR IAASCLERVTMGDTLHKETV a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a

7500 105 100 95 90 PSYEVLDNQSYHIRRGLQEQ AmpR PSYEVLDNQSYHIRRGLQEQ g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g

7560 85 80 75 70 GADVRSLVAGCLLVKFTSMM AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GADVRSLVAGCLLVKFTSMM c c g g c g t c a a c a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g c c g c a g t t g t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a

7620 65 60 55 50 PFREEPRFSELIKGSNLDLE AmpR PFREEPRFSELIKGSNLDLE g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c

7680 45 40 35 30 IYGVRAGLQDEADKVKVLTE AmpR IYGVRAGLQDEADKVKVLTE a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a

7740 25 PH 20 15 10 signal sequence AFVPLCFAAFFPILAVRF AmpR PHAFVPLCFAAFFPILAVRF g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t

7800 5 1 signal sequence HQISM AmpR AmpR promoter HQISM t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a

7860 AmpR promoter c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g

7920 a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g

3ʹ 5ʹ 7953 A A T A G G C G T A T C A C G A G G C pBRforEco t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.