Addgene pRSFDuetG-HsDCP1a_1-135 (MCSII)_E - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRSFDuetG-HsDCP1a_1-135 (MCSII)_E

Map View

T7 promoter PfoI (4858) AclI (4766) BstAPI (4750) LacI-R (4743 .. 4762) MluI (4426) BstEII (4244) HpaI (3924) PluTI (3791) SfoI (3789) NarI * (3788) KasI (3787) XbaI (3506) BssSI - BssSαI (3036) pBR322ori-F (3004 .. 3023) L4440 (2764 .. 2781) AfeI (2750) SphI (2746) NruI (2557) Kan-R (2554 .. 2573) BspDI - ClaI (2521) 6xHis BamHI (136) EcoRI (142) Eco53kI (150) SacI (152) BfuAI - BspMI (154) AscI (155) PstI - SbfI (165) SalI (167) NotI (180) AflII (193) T7 promoter MscI (540) BstBI (730) SwaI (760) ScaI (906) pGEX 5' (944 .. 966) NdeI (1018) BglII (1025) PpuMI (1186) BseRI (1428) Acc65I (1440) KpnI (1444) PaeR7I - PspXI - XhoI (1446) PacI (1521) AvrII (1525) T7 Term (1540 .. 1558) Bsu36I (1609) AgeI (1658) DrdI (1729) BsaI (1737) Bpu10I (2194) TspMI - XmaI (2338) SmaI (2340) pRSFDuetG-HsDCP1a_1-135 (MCSII)_E 4921 bp

Sequence View

5ʹ 3ʹ 60 T7 promoter lac operator SNHASLLEGTSIIKNLKLL g g g g a a t t g t g a g c g g a t a a c a a t t c c c c t g t a g a a a t a a t t t t g t t t a a c t t t a a t a a g c c c c t t a a c a c t c g c c t a t t g t t a a g g g g a c a t c t t t a t t a a a a c a a a t t g a a a t t a t t c

120 1 5 HHHHHH SAG 1 LEVL 6xHis HRV 3C site SIYWPCCGDGDDGCRPDPLE g a g a t a t a c c a t g g g c a g c a g c c a t c a c c a t c a t c a c c a c a g c g c g g g t c t g g a a g t t c t c t c t a t a t g g t a c c c g t c g t c g g t a g t g g t a g t a g t g g t g t c g c g c c c a g a c c t t c a a g a

SbfIPstISalIBamHIEcoRIEco53kISacIBspMIBfuAIAscI 180 5 FQGP (in frame with HRV 3C site) QDPNSSSARLQVDKLA HRV 3C site TGPAGPDSNSSPAGAPRCAQ g t t c c a g g g g c c c c a g g a t c c g a a t t c g a g c t c g g c g c g c c t g c a g g t c g a c a a g c t t g c c a a g g t c c c c g g g g t c c t a g g c t t a a g c t c g a g c c g c g c g g a c g t c c a g c t g t t c g a a c g

AflIINotI 240 AA* PRM g g c c g c a t a a t g c t t a a g t c g a a c a g a a a g t a a t c g t a t t g t a c a c g g c c g c a t a a t c g a c c g g c g t a t t a c g a a t t c a g c t t g t c t t t c a t t a g c a t a a c a t g t g c c g g c g t a t t a g c t

300 T7 promoter lac operator a a t t a a t a c g a c t c a c t a t a g g g g a a t t g t g a g c g g a t a a c a a t t c c c c a t c t t a g t a t a t t a a t t a t g c t g a g t g a t a t c c c c t t a a c a c t c g c c t a t t g t t a a g g g g t a g a a t c a t a t

360 1 5 MSPILGYWK GST MSPILGYWK t t a g t t a a g t a t a a g a a g g a g a t a t a c a t a a t a t g t c c c c t a t a c t a g g t t a t t g g a a a a a a t c a a t t c a t a t t c t t c c t c t a t a t g t a t t a t a c a g g g g a t a t g a t c c a a t a a c c t t t t

420 10 15 20 25 IKGLVQPTRLLLEYLEEKYE GST IKGLVQPTRLLLEYLEEKYE t t a a g g g c c t t g t g c a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a a t t c c c g g a a c a c g t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c

480 30 35 40 45 EHLYERDEGDKWRNKKFELG GST EHLYERDEGDKWRNKKFELG a g c a t t t g t a t g a g c g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t c g t a a a c a t a c t c g c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a

MscI 540 50 55 60 65 LEFPNLPYYIDGDVKLTQSM GST LEFPNLPYYIDGDVKLTQSM t g g a g t t t c c c a a t c t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g a c c t c a a a g g g t t a g a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c

600 70 75 80 85 AIIRYIADKHNMLGGCPKER GST AIIRYIADKHNMLGGCPKER c c a t c a t a c g t t a t a t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g g g t a g t a t g c a a t a t a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c

660 90 95 100 105 AEISMLEGAVLDIRYGVSRI GST AEISMLEGAVLDIRYGVSRI c a g a g a t t t c a a t g c t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g g t c t c t a a a g t t a c g a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c

720 110 115 120 125 AYSKDFETLKVDFLSKLPEM GST AYSKDFETLKVDFLSKLPEM c a t a t a g t a a a g a c t t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c g t a t a t c a t t t c t g a a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g

SwaIBstBI 780 130 135 140 145 LKMFEDRLCHKTYLNGDHVT GST LKMFEDRLCHKTYLNGDHVT t g a a a a t g t t c g a a g a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a c t t t t a c a a g c t t c t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g

840 150 155 160 165 HPDFMLYDALDVVLYMDPMC GST HPDFMLYDALDVVLYMDPMC a t c c t g a c t t c a t g t t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t a g g a c t g a a g t a c a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g

900 170 175 180 185 LDAFPKLVCFKKRIEAIPQI GST LDAFPKLVCFKKRIEAIPQI t g g a t g c g t t c c c a a a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a c c t a c g c a a g g g t t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c

ScaI 960 190 195 200 205 DKYLKSSKYIAWPLQGWQAT GST G G G C T G G C A A G C C A C G T pGEX 5' DKYLKSSKYIAWPLQGWQAT a t a a g t a c t t g a a a t c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t a t t c a t g a a c t t t a g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a

NdeI 1020 210 215 FGGGDHPPK SD 1 5 LEVLFQGP H GST HRV 3C site T T G G T G pGEX 5' FGGGDHPPKSDLEVLFQGPH t t g g t g g t g g c g a c c a t c c t c c a a a a t c g g a t c t g g a a g t t c t g t t c c a g g g g c c c c a t a a a c c a c c a c c g c t g g t a g g a g g t t t t a g c c t a g a c c t t c a a g a c a a g g t c c c c g g g g t a t

BglII 1080 (in frame with HRV 3C site) MADLMEALSRAGQEMSLAAL MADLMEALSRAGQEMSLAAL t g g c a g a t c t c a t g g a g g c g c t g a g t c g a g c t g g g c a g g a g a t g a g c c t a g c g g c c c t g a a c c g t c t a g a g t a c c t c c g c g a c t c a g c t c g a c c c g t c c t c t a c t c g g a t c g c c g g g a c t

1140 (in frame with HRV 3C site) KQHDPYITSIADLTGQVALY KQHDPYITSIADLTGQVALY a g c a a c a c g a c c c c t a t a t c a c c a g c a t c g c a g a c c t c a c g g g c c a g g t c g c t c t g t a c a t c g t t g t g c t g g g g a t a t a g t g g t c g t a g c g t c t g g a g t g c c c g g t c c a g c g a g a c a t g t

PpuMI 1200 (in frame with HRV 3C site) TFCPKANQWEKTDIEGTLFV TFCPKANQWEKTDIEGTLFV c c t t c t g c c c c a a g g c c a a c c a g t g g g a g a a g a c t g a t a t a g a a g g g a c c t t a t t c g t a t g g a a g a c g g g g t t c c g g t t g g t c a c c c t c t t c t g a c t a t a t c t t c c c t g g a a t a a g c a t a

1260 (in frame with HRV 3C site) YRRSASPYHGFTIVNRLNMH YRRSASPYHGFTIVNRLNMH a t c g a a g g t c a g c t t c c c c t t a c c a t g g t t t t a c c a t t g t g a a t c g a c t a a a t a t g c a c a t a g c t t c c a g t c g a a g g g g a a t g g t a c c a a a a t g g t a a c a c t t a g c t g a t t t a t a c g t g t

1320 (in frame with HRV 3C site) NLVEPVNKDLEFQLHEPFLL NLVEPVNKDLEFQLHEPFLL a t c t a g t t g a a c c a g t g a a t a a a g a t t t g g a a t t t c a g c t c c a t g a a c c a t t t c t t c t g t t a g a t c a a c t t g g t c a c t t a t t t c t a a a c c t t a a a g t c g a g g t a c t t g g t a a a g a a g a c a

1380 (in frame with HRV 3C site) YRNASLSIYSIWFYDKNDCH YRNASLSIYSIWFYDKNDCH a t a g a a a t g c a a g c t t g t c g a t a t a t a g t a t c t g g t t t t a t g a c a a g a a t g a c t g t c a c c t a t c t t t a c g t t c g a a c a g c t a t a t a t c a t a g a c c a a a a t a c t g t t c t t a c t g a c a g t g g

BseRI 1440 (in frame with HRV 3C site) RIAKLMADVVEEETRRSQQ* RIAKLMADVVEEETRRSQQ* g c a t a g c a a a a c t c a t g g c t g a t g t g g t a g a a g a g g a g a c a c g g c g a t c c c a g c a a t a g g c g t a t c g t t t t g a g t a c c g a c t a c a c c a t c t t c t c c t c t g t g c c g c t a g g g t c g t t a t c c

Acc65IKpnIPaeR7IPspXIXhoI 1500 1 5 10 KETAAAKFERQHMD S-Tag g t a c c c t c g a g t c t g g t a a a g a a a c c g c t g c t g c g a a a t t t g a a c g c c a g c a c a t g g a c t c a t g g g a g c t c a g a c c a t t t c t t t g g c g a c g a c g c t t t a a a c t t g c g g t c g t g t a c c t g a

AvrIIPacI 1560 15 S (in frame with S-Tag) STSAA* S-Tag T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term c g t c t a c t a g c g c a g c t t a a t t a a c c t a g g c t g c t g c c a c c g c t g a g c a a t a a c t a g c a t g c a g a t g a t c g c g t c g a a t t a a t t g g a t c c g a c g a c g g t g g c g a c t c g t t a t t g a t c g t a

Bsu36I 1620 T7 terminator a a c c c c t t g g g g c c t c t a a a c g g g t c t t g a g g g g t t t t t t g c t g a a a c c t c a g g c a t t t g t t g g g g a a c c c c g g a g a t t t g c c c a g a a c t c c c c a a a a a a c g a c t t t g g a g t c c g t a a a c

AgeI 1680 a g a a g c a c a c g g t c a c a c t g c t t c c g g t a g t c a a t a a a c c g g t a a a c c a g c a a t a g a c a t t c t t c g t g t g c c a g t g t g a c g a a g g c c a t c a g t t a t t t g g c c a t t t g g t c g t t a t c t g t a

BsaIDrdI 1740 a a g c g g c t a t t t a a c g a c c c t g c c c t g a a c c g a c g a c a a g c t g a c g a c c g g g t c t c c g c a t t c g c c g a t a a a t t g c t g g g a c g g g a c t t g g c t g c t g t t c g a c t g c t g g c c c a g a g g c g t

1800 a g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a

1860 270 265 *FFEDLMLHF KanR 270 265 *FFEDLMLHF KanR *FFEDLMLHF t c a a a t a t g t a t c c g c t c a t g a a t t a a t t c t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a a g t t t a t a c a t a g g c g a g t a c t t a a t t a a g a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t

1920 260 255 250 245 QLKNMDPNDIGYKQFLRKQL KanR 260 255 250 245 QLKNMDPNDIGYKQFLRKQL KanR QLKNMDPNDIGYKQFLRKQL c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t

1980 240 235 230 225 SPSFEGLCNWLIALDQYRDA KanR 240 235 230 225 SPSFEGLCNWLIALDQYRDA KanR SPSFEGLCNWLIALDQYRDA t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g

2040 220 215 210 205 IGVRGVDICGILKGEDFILN KanR 220 215 210 205 IGVRGVDICGILKGEDFILN KanR IGVRGVDICGILKGEDFILN g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a

2100 200 195 190 185 DLSFDGHTVVSDPSFPLLKH KanR 200 195 190 185 DLSFDGHTVVSDPSFPLLKH KanR DLSFDGHTVVSDPSFPLLKH a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t a t g t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a t a c

2160 180 175 170 165 MEKWVQEVPWGNREDDFDSA KanR 180 175 170 165 MEKWVQEVPWGNREDDFDSA KanR MEKWVQEVPWGNREDDFDSA c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g

Bpu10I 2220 160 155 150 145 DVLGNNMRSQAQALRFVRDS KanR 160 155 150 145 DVLGNNMRSQAQALRFVRDS KanR DVLGNNMRSQAQALRFVRDS a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g g t c g c t t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c c a g c g a

2280 140 135 130 125 NFPCNCVPISHLRRLFVALA KanR 140 135 130 125 NFPCNCVPISHLRRLFVALA KanR NFPCNCVPISHLRRLFVALA g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g

TspMIXmaISmaI 2340 120 115 110 105 DVINEGSDPYEELVQFATKG KanR 120 115 110 105 DVINEGSDPYEELVQFATKG KanR DVINEGSDPYEELVQFATKG a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g

2400 100 95 90 85 PIATTLLWADDPTRIFHKIT KanR 100 95 90 85 PIATTLLWADDPTRIFHKIT KanR PIATTLLWADDPTRIFHKIT g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a

2460 80 75 70 65 PLPMFETLWNLRVMEDTVDN KanR 80 75 70 65 PLPMFETLWNLRVMEDTVDN KanR PLPMFETLWNLRVMEDTVDN c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a

2520 60 55 50 45 AVSGKGHKLFLEPADPKGYL KanR 60 55 50 45 AVSGKGHKLFLEPADPKGYL KanR AVSGKGHKLFLEPADPKGYL g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t

NruIBspDIClaI 2580 40 35 30 25 RYITAGSQGVNDRAWKYGYL KanR 40 35 30 25 RYITAGSQGVNDRAWKYGYL KanR A T C G C G A G C C C A T T T A T A C C Kan-R RYITAGSQGVNDRAWKYGYL t c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t

2640 20 15 10 5 DADMNSNLRPRSCSTERQIH KanR 20 15 10 5 DADMNSNLRPRSCSTERQIH KanR DADMNSNLRPRSCSTERQIH a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t a g a g c a a g a c g t t t c c c g t t g a a t a t g t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a t c t c g t t c t g c a a a g g g c a a c t t a t a c

2700 1 SM KanR AmpR promoter 1 SM KanR SM g c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c

AfeISphI 2760 AmpR promoter RSF ori c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g c a t g c a g c g c t c t t c c g c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c g t a c g t c g c g a g a a g g c g

2820 RSF ori G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t a c g c t c g g t c g t t c g a c t g c g g c g a g c g g t g t c a g c t c a a a g g a g c g a g t g a c t g a g c g a t g c g a g c c a g c a a g c t g a c g c c g c t c g c c a c a g t c g a g t

2880 RSF ori c t c a a a a g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a a g c c g g a a a g a a c a t g t g g a g t t t t c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t t c g g c c t t t c t t g t a c a c

2940 RSF ori a g c a a a a a g c a a a g c a c c g g a a g a a g c c a a c g c c g c a g g c g t t t t t c c a t a g g c t c c g c c t c g t t t t t c g t t t c g t g g c c t t c t t c g g t t g c g g c g t c c g c a a a a a g g t a t c c g a g g c g g

3000 RSF ori c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g c c a g a g g t g g c g a a a c c c g a c a g g a c g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c g g t c t c c a c c g c t t t g g g c t g t c c t g

BssSαIBssSI 3060 RSF ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g

3120 RSF ori t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t

3180 RSF ori g c t c a c g c t g t t g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c c g a g t g c g a c a a c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g

3240 RSF ori a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t

3300 RSF ori a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a t t g g t a a c t g a t t t a g a g g a c t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t a a c c a t t g a c t a a a t c t c c t g

3360 RSF ori t t t g t c t t g a a g t t a t g c a c c t g t t a a g g c t a a a c t g a a a g a a c a g a t t t t g g t g a g t g c a a a c a g a a c t t c a a t a c g t g g a c a a t t c c g a t t t g a c t t t c t t g t c t a a a a c c a c t c a c g

3420 RSF ori g g t c c t c c a a c c c a c t t a c c t t g g t t c a a a g a g t t g g t a g c t c a g c g a a c c t t g a g a a a a c c a g g a g g t t g g g t g a a t g g a a c c a a g t t t c t c a a c c a t c g a g t c g c t t g g a a c t c t t t t

3480 RSF ori c c a c c g t t g g t a g c g g t g g t t t t t c t t t a t t t a t g a g a t g a t g a a t c a a t c g g t c t a t c a g g t g g c a a c c a t c g c c a c c a a a a a g a a a t a a a t a c t c t a c t a c t t a g t t a g c c a g a t a g t

XbaI 3540 RSF ori a g t c a a c g a a c a g c t a t t c c g t t a c t c t a g a t t t c a g t g c a a t t t a t c t c t t c a a a t g t a t c a g t t g c t t g t c g a t a a g g c a a t g a g a t c t a a a g t c a c g t t a a a t a g a g a a g t t t a c a t

3600 g c a c c t g a a g t c a g c c c c a t a c g a t a t a a g t t g t a a t t c t c a t g t t a g t c a t g c c c c g c g c g t g g a c t t c a g t c g g g g t a t g c t a t a t t c a a c a t t a a g a g t a c a a t c a g t a c g g g g c g c

3660 c c c a c c g g a a g g a g c t g a c t g g g t t g a a g g c t c t c a a g g g c a t c g g t c g a g a t c c c g g t g g g g t g g c c t t c c t c g a c t g a c c c a a c t t c c g a g a g t t c c c g t a g c c a g c t c t a g g g c c a c

3720 360 355 *QGSELR lacI *QGSELR c c t a a t g a g t g a g c t a a c t t a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g g a t t a c t c a c t c g a t t g a a t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c

3780 350 345 340 335 SVQRALQMLSDALARPSATQ lacI SVQRALQMLSDALARPSATQ g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g

KasINarI*SfoIPluTI 3840 330 325 320 315 TNPALTTKRKVLSVPLLQNG lacI TNPALTTKRKVLSVPLLQNG g t a t t g g g c g c c a g g g t g g t t t t t c t t t t c a c c a g t g a g a c g g g c a a c a g c t g a t t g c c c c a t a a c c c g c g g t c c c a c c a a a a a g a a a a g t g g t c a c t c t g c c c g t t g t c g a c t a a c g g g

3900 310 305 300 295 KVAQGQSLQLLRDVSTQGLL lacI KVAQGQSLQLLRDVSTQGLL t t c a c c g c c t g g c c c t g a g a g a g t t g c a g c a a g c g g t c c a c g c t g g t t t g c c c c a g c a g g a a g t g g c g g a c c g g g a c t c t c t c a a c g t c g t t c g c c a g g t g c g a c c a a a c g g g g t c g t c c

HpaI 3960 290 285 280 275 RFDQKITTLPPIYCSSDETD lacI RFDQKITTLPPIYCSSDETD c g a a a a t c c t g t t t g a t g g t g g t t a a c g g c g g g a t a t a a c a t g a g c t g t c t t c g g t a t c g g c t t t t a g g a c a a a c t a c c a c c a a t t g c c g c c c t a t a t t g t a c t c g a c a g a a g c c a t a g c

4020 270 265 260 255 DYGVVSIDAGVRLGSETIAR lacI DYGVVSIDAGVRLGSETIAR t c g t a t c c c a c t a c c g a g a t g t c c g c a c c a a c g c g c a g c c c g g a c t c g g t a a t g g c g c g c a g c a t a g g g t g a t g g c t c t a c a g g c g t g g t t g c g c g t c g g g c c t g a g c c a t t a c c g c g c g

4080 250 245 240 235 MAGLAMQDNAVLMATPVIGE lacI MAGLAMQDNAVLMATPVIGE a t t g c g c c c a g c g c c a t c t g a t c g t t g g c a a c c a g c a t c g c a g t g g g a a c g a t g c c c t c a t a a c g c g g g t c g c g g t a g a c t a g c a a c c g t t g g t c g t a g c g t c a c c c t t g c t a c g g g a g t

4140 230 225 220 215 NLMQMTQQFGSMASWDGERE lacI NLMQMTQQFGSMASWDGERE t t c a g c a t t t g c a t g g t t t g t t g a a a a c c g g a c a t g g c a c t c c a g t c g c c t t c c c g t t c c a a g t c g t a a a c g t a c c a a a c a a c t t t t g g c c t g t a c c g t g a g g t c a g c g g a a g g g c a a g g

4200 210 205 200 195 AIPQIQNRTLYKHWGALRLR lacI AIPQIQNRTLYKHWGALRLR g c t a t c g g c t g a a t t t g a t t g c g a g t g a g a t a t t t a t g c c a g c c a g c c a g a c g c a g a c g c c g a t a g c c g a c t t a a a c t a a c g c t c a c t c t a t a a a t a c g g t c g g t c g g t c t g c g t c t g c g

BstEII 4260 190 185 180 175 ASVSSLPGALLAIQQHGLAV lacI ASVSSLPGALLAIQQHGLAV g c c g a g a c a g a a c t t a a t g g g c c c g c t a a c a g c g c g a t t t g c t g g t g a c c c a a t g c g a c c c g g c t c t g t c t t g a a t t a c c c g g g c g a t t g t c g c g c t a a a c g a c c a c t g g g t t a c g c t g g

4320 170 165 160 155 LHEVGLRTGDEHSFIISNIP lacI LHEVGLRTGDEHSFIISNIP a g a t g c t c c a c g c c c a g t c g c g t a c c g t c t t c a t g g g a g a a a a t a a t a c t g t t g a t g g g t t c t a c g a g g t g c g g g t c a g c g c a t g g c a g a a g t a c c c t c t t t t a t t a t g a c a a c t a c c c a

4380 150 145 140 135 TQDSVDLFLAPVNTCAAEVA lacI TQDSVDLFLAPVNTCAAEVA g t c t g g t c a g a g a c a t c a a g a a a t a a c g c c g g a a c a t t a g t g c a g g c a g c t t c c a c a g c a c a g a c c a g t c t c t g t a g t t c t t t a t t g c g g c c t t g t a a t c a c g t c c g t c g a a g g t g t c g t

MluI 4440 130 125 120 115 IADQDDLPYNIILGSVRQAL lacI IADQDDLPYNIILGSVRQAL a t g g c a t c c t g g t c a t c c a g c g g a t a g t t a a t g a t c a g c c c a c t g a c g c g t t g c g c g a g a t a c c g t a g g a c c a g t a g g t c g c c t a t c a a t t a c t a g t c g g g t g a c t g c g c a a c g c g c t c t

4500 110 105 100 95 LNHVAAKCAEVGSREVMSVV lacI LNHVAAKCAEVGSREVMSVV a g a t t g t g c a c c g c c g c t t t a c a g g c t t c g a c g c c g c t t c g t t c t a c c a t c g a c a c c a c c t c t a a c a c g t g g c g g c g a a a t g t c c g a a g c t g c g g c g a a g c a a g a t g g t a g c t g t g g t g g

4560 90 85 80 75 VSAGLQDARSKIAAVIQSPA lacI VSAGLQDARSKIAAVIQSPA *DGRCNAVAR a c g c t g g c a c c c a g t t g a t c g g c g c g a g a t t t a a t c g c c g c g a c a a t t t g c g a c g g c g c g t g c g a c c g t g g g t c a a c t a g c c g c g c t c t a a a t t a g c g g c g c t g t t a a a c g c t g c c g c g c

4620 70 65 60 55 HLALSSTAVGILLSQKGALQ lacI HLALSSTAVGILLSQKGALQ APGSQLHCRWDAVVTQGGTT t g c a g g g c c a g a c t g g a g g t g g c a a c g c c a a t c a g c a a c g a c t g t t t g c c c g c c a g t t g t a c g t c c c g g t c t g a c c t c c a c c g t t g c g g t t a g t c g t t g c t g a c a a a c g g g c g g t c a a c a

4680 50 45 40 35 QAVRNPIYNLEAMAAEVKER lacI QAVRNPIYNLEAM TGRPQSHLEAGGDGSGSKGA t g t g c c a c g c g g t t g g g a a t g t a a t t c a g c t c c g c c a t c g c c g c t t c c a c t t t t t c c c g c a c a c g g t g c g c c a a c c c t t a c a t t a a g t c g a g g c g g t a g c g g c g a a g g t g a a a a a g g g c g

4740 30 25 20 15 TKASVHSAQNVVRSVTQYSV lacI NECFRPQGPEGRPFRDSLLC g t t t t c g c a g a a a c g t g g c t g g c c t g g t t c a c c a c g c g g g a a a c g g t c t g a t a a g a g a c a c a a a a g c g t c t t t g c a c c g a c c g g a c c a a g t g g t g c g c c c t t t g c c a g a c t a t t c t c t g t

AclIBstAPI 4800 10 5 1 GAYEAVDYLTVPKM lacI lacI promoter G G C A T A C T C T G C G A C A T C G T LacI-R RCVRRCRIVNSTECEGGQIS c c g g c a t a c t c t g c g a c a t c g t a t a a c g t t a c t g g t t t c a c a t t c a c c a c c c t g a a t t g a g g c c g t a t g a g a c g c t g t a g c a t a t t g c a a t g a c c a a a g t g t a a g t g g t g g g a c t t a a c t

PfoI 4860 lacI promoter ERGPAIMGYRSLNQAMRHHG c t c t c t t c c g g g c g c t a t c a t g c c a t a c c g c g a a a g g t t t t g c g c c a t t c g a t g g t g t c c g a g a g a a g g c c c g c g a t a g t a c g g t a t g g c g c t t t c c a a a a c g c g g t a a g c t a c c a c a g g

4920 T7 promoter PDRREGKHSEQMLFNIRSVI g g g a t c t c g a c g c t c t c c c t t a t g c g a c t c c t g c a t t a g g a a a t t a a t a c g a c t c a c t a t c c c t a g a g c t g c g a g a g g g a a t a c g c t g a g g a c g t a a t c c t t t a a t t a t g c t g a g t g a t a

3ʹ 5ʹ 4921 T7 promoter a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.