Addgene pAc5.1C-Fluc-CG3548_A - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAc5.1C-Fluc-CG3548_A


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BglII (132) Amp-R (6903 .. 6922) PstI (6598) NmeAIII (6501) AhdI (6353) pBR322ori-F (5601 .. 5620) DrdI (5568) EagI (5295) PshAI (5072) SalI (5007) PaqCI (4916) EcoRI (4244) EcoNI (4208) SgrAI (4019) AsiSI (391) TspMI - XmaI (425) SmaI (427) BbvCI (502) KasI (1388) NarI (1389) SfoI (1390) PluTI (1392) BsaBI (1769) BclI * (1820) AflII (1934) SpeI (1958) BtgI - NcoI - StyI (2261) BlpI (2439) Acc65I (2579) KpnI (2583) LucNrev (2657 .. 2675) PfoI * (2698) BsaAI (2745) BstEII (3198) Bsu36I (3204) XcmI (3326) pAc5.1C-Fluc-CG3548_A 7338 bp

Sequence View

60 Ac5 promoter c t t c a a g g a a t t a a t t c t a t a t t c t a a a a a c a c a a a t g a t a c t t c t a a a a a a a a t c a t g a g a a g t t c c t t a a t t a a g a t a t a a g a t t t t t g t g t t t a c t a t g a a g a t t t t t t t t a g t a c t

120 Ac5 promoter a t g g c a t c a a c t c t g a a t c a a a t c t t t g c a g a t g c a c c t a c t t c t c a t t t c c a c t g t c a c t a c c g t a g t t g a g a c t t a g t t t a g a a a c g t c t a c g t g g a t g a a g a g t a a a g g t g a c a g t g

BglII 180 Ac5 promoter a t c a t t t t t c c a g a t c t c g c t g c c t g t t a t g t g g c c c a c a a a c c a a g a c a c g t t t t a t g g t a g t a a a a a g g t c t a g a g c g a c g g a c a a t a c a c c g g g t g t t t g g t t c t g t g c a a a a t a c c

240 Ac5 promoter c c a t t a a a g c t g g c t g a t c g t c g c c a a a c a c c a a a t a c a t a t c a a t a t g t a c a t t c g a g a g g t a a t t t c g a c c g a c t a g c a g c g g t t t g t g g t t t a t g t a t a g t t a t a c a t g t a a g c t c t

300 Ac5 promoter a a g a a g c g a t c a a a g a a g c g t c t t c g g g c g a g t a g g a g a a t g c g g a g g a g a a g g a g a a c g t t c t t c g c t a g t t t c t t c g c a g a a g c c c g c t c a t c c t c t t a c g c c t c c t c t t c c t c t t g c

360 Ac5 promoter a g c t g a t c t a g t a t c t c t c c a c a a t c c a a t g c c a a c t g a c c a a c t g g c c a t a t t c g g a g c t c g a c t a g a t c a t a g a g a g g t g t t a g g t t a c g g t t g a c t g g t t g a c c g g t a t a a g c c t c g

AsiSI 420 Ac5 promoter a a t t t g a a g c c a a t t t c c a t c g c c t g g c g a t c g c t c c a t t c t t g g c t a t a t g t t t t t c a c t t a a a c t t c g g t t a a a g g t a g c g g a c c g c t a g c g a g g t a a g a a c c g a t a t a c a a a a a g t g

TspMIXmaISmaI 480 Ac5 promoter c g t t c c c g g g g c c a t t t t c a a a g a c t c g t c g g t a a g a t a a g a t t g t g t c a c t c g c t g t c t g c a a g g g c c c c g g t a a a a g t t t c t g a g c a g c c a t t c t a t t c t a a c a c a g t g a g c g a c a g a

BbvCI 540 Ac5 promoter c t c t t c a t t t g t c g a a g a a t g c t g a g g a a t t t c g c g a t g a c g t c g g c g a g t a t t t t g a a g g a g a a g t a a a c a g c t t c t t a c g a c t c c t t a a a g c g c t a c t g c a g c c g c t c a t a a a a c t t c

600 Ac5 promoter a a t g a g a a t a a t t t g t a t t t a t a c g a a a a t c a g t t a g t g g a a t t t t c t a c a a a a a c a t g t t t a c t c t t a t t a a a c a t a a a t a t g c t t t t a g t c a a t c a c c t t a a a a g a t g t t t t t g t a c a

660 Ac5 promoter t a t c t a t a g a t a a t t t t g t t g c a a a a t a t g t t g a c t a t g a c a a a g a t t g t a t g t a t a t a c a t a g a t a t c t a t t a a a a c a a c g t t t t a t a c a a c t g a t a c t g t t t c t a a c a t a c a t a t a t g

720 Ac5 promoter c t t t a a t g t a t t c t c a t t t t c t t a t g t a t t t a t a a t g g c a a t g a t g a t a c t g a t g a t a t t g a a a t t a c a t a a g a g t a a a a g a a t a c a t a a a t a t t a c c g t t a c t a c t a t g a c t a c t a t a a

780 Ac5 promoter MMPDHRLISASFAERSAC t t a a g a t g a t g c c a g a c c a c a g g c t g a t t t c t g c g t c t t t t g c c g a a c g c a g t g c a t g t g a a t t c t a c t a c g g t c t g g t g t c c g a c t a a a g a c g c a g a a a a c g g c t t g c g t c a c g t a c a c

840 Ac5 promoter AVVVFWNSFNFRTVRFDFSF c g g t t g t t g t t t t t t g g a a t a g t t t c a a t t t t c g g a c t g t c c g c t t t g a t t t c a g t t t c t g c c a a c a a c a a a a a a c c t t a t c a a a g t t a a a a g c c t g a c a g g c g a a a c t a a a g t c a a a g a

900 Ac5 promoter LAYSKSKVKPKKRDGNTKCG t g g c t t a t t c a a a a a g c a a a g t a a a g c c a a a a a a g c g a g a t g g c a a t a c c a a a t g c g g c a a c c g a a t a a g t t t t t c g t t t c a t t t c g g t t t t t t c g c t c t a c c g t t a t g g t t t a c g c c g t

960 Ac5 promoter KTVVEGKGCGAAEGRVGRGV a a a c g g t a g t g g a a g g a a a g g g g t g c g g g g c a g c g g a a g g a a g g g t g g g g c g g g g c g t g g t t t g c c a t c a c c t t c c t t t c c c c a c g c c c c g t c g c c t t c c t t c c c a c c c c g c c c c g c a c c

1020 Ac5 promoter AGSVAGRDVTDVGATPLTMC c g g g g t c t g t g g c t g g g c g c g a c g t c a c c g a c g t t g g a g c c a c t c c t t t g a c c a t g t g t g g c c c c a g a c a c c g a c c c g c g c t g c a g t g g c t g c a a c c t c g g t g a g g a a a c t g g t a c a c a c

1080 Ac5 promoter ACVYYSCLATRRLFFSFYLA c g t g t g t g t a t t a t t c g t g t c t c g c c a c t c g c c g g t t g t t t t t t t c t t t t t a t c t c g c t c g c a c a c a c a t a a t a a g c a c a g a g c g g t g a g c g g c c a a c a a a a a a a g a a a a a t a g a g c g a g

1140 Ac5 promoter LSSAISYACSTHRMLPCPLC t c t c t a g c g c c a t c t c g t a c g c a t g c t c a a c g c a c c g c a t g t t g c c g t g t c c t t t a t g c g a g a g a t c g c g g t a g a g c a t g c g t a c g a g t t g c g t g g c g t a c a a c g g c a c a g g a a a t a c g c

1200 Ac5 promoter VILARNRQLFKQRLVNENAK t c a t t t t g g c t c g a a a t a g g c a a t t a t t t a a a c a a a g a t t a g t c a a c g a a a a c g c t a a a a a g t a a a a c c g a g c t t t a t c c g t t a a t a a a t t t g t t t c t a a t c a g t t g c t t t t g c g a t t t t

1260 Ac5 promoter INKSTIWLLIAMCVQPTIAT t a a a t a a g t c t a c a a t a t g g t t a c t t a t t g c c a t g t g t g t g c a g c c a a c g a t a g c a a c a a a t t t a t t c a g a t g t t a t a c c a a t g a a t a a c g g t a c a c a c a c g t c g g t t g c t a t c g t t g t t

1320 Ac5 promoter KATTQWLSLFHFLFASACEQ a a g c a a c a a c a c a g t g g c t t t c c c t c t t t c a c t t t t t g t t t g c a a g c g c g t g c g a g c a a g t t c g t t g t t g t g t c a c c g a a a g g g a g a a a g t g a a a a a c a a a c g t t c g c g c a c g c t c g t t c

1380 Ac5 promoter DGTTGKRNYADKEQTKFWPK a c g g c a c g a c c g g c a a a c g c a a t t a c g c t g a c a a a g a g c a g a c g a a g t t t t g g c c g a a a a t g c c g t g c t g g c c g t t t g c g t t a a t g c g a c t g t t t c t c g t c t g c t t c a a a a c c g g c t t t t

KasINarISfoIPluTI 1440 Ac5 promoter NIKAPDTNAFAITIAIFNIV a c a t c a a g g c g c c t g a t a c g a a t g c a t t t g c a a t a a c a a t t g c g a t a t t t a a t a t t g t t t t g t a g t t c c g c g g a c t a t g c t t a c g t a a a c g t t a t t g t t a a c g c t a t a a a t t a t a a c a a a

1500 Ac5 promoter YEAV* a t g a a g c t g t t t g a c t t c a a a a c a c a c a a a a a a a a a a a t a a a a c a a a t t a t t t g a a a g a g t a c t t c g a c a a a c t g a a g t t t t g t g t g t t t t t t t t t t t a t t t t g t t t a a t a a a c t t t c t c

1560 Ac5 promoter a a t t a g g a a t c g g a c a g c t t a t c g t t a c g g g c t a a c a g c a c a c c g a g a c g a a a t a g c t t a t t a a t c c t t a g c c t g t c g a a t a g c a a t g c c c g a t t g t c g t g t g g c t c t g c t t t a t c g a a t

1620 Ac5 promoter c c t g a c g t c a c a g c c t c t g g a a g a a c t g c c g c c a a g c a g a g a g a g a g a g a a a a a g a g g g a g g a c t g c a g t g t c g g a g a c c t t c t t g a c g g c g g t t c g t c t c t c t c t c t c t t t t t c t c c c t

1680 Ac5 promoter g a g c a g c t t a g a c c g c a t g t g c t t g t g t g t g a g g c g t c t c t c t c t t c g t c t c c t g t t t g c c t c g t c g a a t c t g g c g t a c a c g a a c a c a c a c t c c g c a g a g a g a g a a g c a g a g g a c a a a c g

1740 Ac5 promoter g c a a a c g c a t a g a c t g c a c t g a g a a a a t c g a t t a c c t a t t t t t t a t g a a t g a a t a t t t g c c g t t t g c g t a t c t g a c g t g a c t c t t t t a g c t a a t g g a t a a a a a a t a c t t a c t t a t a a a c g

BsaBI 1800 Ac5 promoter a c t a t t a c t a t t c a a a a c t a t t a a g a t a g c a a t c a c a t t c a a t a g c c a a a t a c t a t a c c a t g a t a a t g a t a a g t t t t g a t a a t t c t a t c g t t a g t g t a a g t t a t c g g t t t a t g a t a t g g t

BclI* 1860 Ac5 promoter c c t g a g c g a t g c a a c g a a a t g a t c a a t t t g a g c a a a a a t g c t g c a t a t t t a g g a c g g c a t g g a c t c g c t a c g t t g c t t t a c t a g t t a a a c t c g t t t t t a c g a c g t a t a a a t c c t g c c g t a

1920 Ac5 promoter c a t t a t a g a a a t g c t t c t t g c t g t g t a c t t t t c t c t c g t c t g g c a g c t g t t t c g c c g t t a g t a a t a t c t t t a c g a a g a a c g a c a c a t g a a a a g a g a g c a g a c c g t c g a c a a a g c g g c a a t

SpeIAflII 1980 Ac5 promoter *THKRRSTIGRSSS t t g t t a a a a c c g g c t t a a g t t a g g t g t g t t t t c t a c g a c t a g t g a t g c c c c t a c t a g a a g a a c a a t t t t g g c c g a a t t c a a t c c a c a c a a a a g a t g c t g a t c a c t a c g g g g a t g a t c t t c

2040 Ac5 promoter THQVFTGSYGIQLASLLLRL a t g t g t g t t g c a c a a a t g t c c c t g a a t a a c c a a t t t g a a g t g c a g a t a g c a g t a a a c g t a t a c a c a c a a c g t g t t t a c a g g g a c t t a t t g g t t a a a c t t c a c g t c t a t c g t c a t t t g c a t

2100 Ac5 promoter SIHINNLQLTKIDSSMVLLG a g c t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c g c t g g a c a t t a c t a a t a a a c t c g a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a g c g a c c t g t a a t g a t t a t t t g

2160 Ac5 promoter VIFVHVYTHKPMCHTTPSFI c c a c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c a a t g a g t a g t t g g g g a a a a a a g g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g t t a c t c a t c a a c c c c t t t t t t

2220 Ac5 promoter HLLVTVMVAYLYGASTDFIL t g t g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c c a g c t g a a g t a t c g a a t a t g a a c a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g g t c g a c t t c a t a g c t t a t a c t

NcoIStyIBtgI 2280 Ac5 promoter LGWISDCAAVSLVWPLVSKM g t a a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a c c c a t g g a a g t a c a c t c t t c a c a t t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t g g g t a c c t t c a t g t g a g a a g t

2340 Ac5 promoter AIYLVCVLVFVWNRLFNETF t g g c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t t g c g g a g g a a a t t c t c c g t a a a c c g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a a c g c c t c c t t t a a g a g g c a t t

2400 Ac5 promoter SFGIPSCNMGMYPLLKSRSK a t g a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g g t a a a a g t t t t g a a c g c g a c t t a c t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c c a t t t t c a a a a c t t g c g c t g a

BlpI 2460 Ac5 promoter LASLM t g a g a g c g g a g a g c a t t g c g g c t g a t a a g g t t t t a g c g c t a a g c g g g c t t t a t a a a a c g g a c t c t c g c c t c t c g t a a c g c c g a c t a t t c c a a a a t c g c g a t t c g c c c g a a a t a t t t t g c c

2520 Ac5 promoter g c t g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c t t g t g c t g t g t g g a t a c t c c t c g a c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g a a c a c g a c a c a c c t a t g a g g a

Acc65I 2580 Ac5 promoter c c c g a c a c a a a g c c g c t c c a t c a g c c a g c a g t c g t c t a a t c c a g a g a c c c c g g a t c g g g g g g g c t g t g t t t c g g c g a g g t a g t c g g t c g t c a g c a g a t t a g g t c t c t g g g g c c t a g c c c c

KpnI 2640 1 5 10 15 MAEDAKNIKKGPAPFYPL luciferase MAEDAKNIKKGPAPFYPL t a c c a a c a t g g c c g a a g a c g c c a a a a a c a t a a a g a a a g g c c c g g c a c c a t t c t a t c c a c t a t g g t t g t a c c g g c t t c t g c g g t t t t t g t a t t t c t t t c c g g g c c g t g g t a a g a t a g g t g a

PfoI* 2700 20 25 30 35 EDGTAGEQLHKAMKRYALVP luciferase C C T C T C G T T G A C G T A T T C C LucNrev EDGTAGEQLHKAMKRYALVP a g a g g a t g g a a c c g c t g g a g a g c a a c t g c a t a a g g c t a t g a a g a g a t a c g c c c t g g t t c c t c t c c t a c c t t g g c g a c c t c t c g t t g a c g t a t t c c g a t a c t t c t c t a t g c g g g a c c a a g g

BsaAI 2760 40 45 50 55 GTIAFTDAHIEVNITYAEYF luciferase GTIAFTDAHIEVNITYAEYF t g g a a c a a t t g c t t t t a c a g a t g c a c a t a t c g a g g t g a a c a t c a c g t a c g c g g a a t a c t t a c c t t g t t a a c g a a a a t g t c t a c g t g t a t a g c t c c a c t t g t a g t g c a t g c g c c t t a t g a a

2820 60 65 70 75 EMSVRLAEAMKRYGLNTNHR luciferase EMSVRLAEAMKRYGLNTNHR c g a a a t g t c c g t t c g g t t g g c a g a a g c t a t g a a a c g a t a t g g g c t g a a t a c a a a t c a c a g g c t t t a c a g g c a a g c c a a c c g t c t t c g a t a c t t t g c t a t a c c c g a c t t a t g t t t a g t g t c

2880 80 85 90 95 IVVCSENSLQFFMPVLGALF luciferase IVVCSENSLQFFMPVLGALF a a t c g t c g t a t g c a g t g a a a a c t c t c t t c a a t t c t t t a t g c c g g t g t t g g g c g c g t t a t t t t a g c a g c a t a c g t c a c t t t t g a g a g a a g t t a a g a a a t a c g g c c a c a a c c c g c g c a a t a a

2940 100 105 110 115 IGVAVAPANDIYNERELLNS luciferase IGVAVAPANDIYNERELLNS t a t c g g a g t t g c a g t t g c g c c c g c g a a c g a c a t t t a t a a t g a a c g t g a a t t g c t c a a c a g a t a g c c t c a a c g t c a a c g c g g g c g c t t g c t g t a a a t a t t a c t t g c a c t t a a c g a g t t g t c

3000 120 125 130 135 MNISQPTVVFVSKKGLQKIL luciferase MNISQPTVVFVSKKGLQKIL t a t g a a c a t t t c g c a g c c t a c c g t a g t g t t t g t t t c c a a a a a g g g g t t g c a a a a a a t t t t a t a c t t g t a a a g c g t c g g a t g g c a t c a c a a a c a a a g g t t t t t c c c c a a c g t t t t t t a a a a

3060 140 145 150 155 NVQKKLPIIQKIIIMDSKTD luciferase NVQKKLPIIQKIIIMDSKTD g a a c g t g c a a a a a a a a t t a c c a a t a a t c c a g a a a a t t a t t a t c a t g g a t t c t a a a a c g g a c t t g c a c g t t t t t t t t a a t g g t t a t t a g g t c t t t t a a t a a t a g t a c c t a a g a t t t t g c c t

3120 160 165 170 175 YQGFQSMYTFVTSHLPPGFN luciferase YQGFQSMYTFVTSHLPPGFN t t a c c a g g g a t t t c a g t c g a t g t a c a c g t t c g t c a c a t c t c a t c t a c c t c c c g g t t t t a a a a t g g t c c c t a a a g t c a g c t a c a t g t g c a a g c a g t g t a g a g t a g a t g g a g g g c c a a a a t t

3180 180 185 190 195 EYDFVPESFDRDKTIALIMN luciferase EYDFVPESFDRDKTIALIMN t g a a t a c g a t t t t g t a c c a g a g t c c t t t g a t c g t g a c a a a a c a a t t g c a c t g a t a a t g a a a c t t a t g c t a a a a c a t g g t c t c a g g a a a c t a g c a c t g t t t t g t t a a c g t g a c t a t t a c t t

Bsu36IBstEII 3240 200 205 210 215 SSGSTGLPKGVALPHRTACV luciferase SSGSTGLPKGVALPHRTACV c t c c t c t g g a t c t a c t g g g t t a c c t a a g g g t g t g g c c c t t c c g c a t a g a a c t g c c t g c g t g a g g a g a c c t a g a t g a c c c a a t g g a t t c c c a c a c c g g g a a g g c g t a t c t t g a c g g a c g c a

3300 220 225 230 235 RFSHARDPIFGNQIIPDTAI luciferase RFSHARDPIFGNQIIPDTAI c a g a t t c t c g c a t g c c a g a g a t c c t a t t t t t g g c a a t c a a a t c a t t c c g g a t a c t g c g a t g t c t a a g a g c g t a c g g t c t c t a g g a t a a a a a c c g t t a g t t t a g t a a g g c c t a t g a c g c t a

XcmI 3360 240 245 250 255 LSVVPFHHGFGMFTTLGYLI luciferase LSVVPFHHGFGMFTTLGYLI t t t a a g t g t t g t t c c a t t c c a t c a c g g t t t t g g a a t g t t t a c t a c a c t c g g a t a t t t g a t a a a t t c a c a a c a a g g t a a g g t a g t g c c a a a a c c t t a c a a a t g a t g t g a g c c t a t a a a c t a

3420 260 265 270 275 CGFRVVLMYRFEEELFLRSL luciferase CGFRVVLMYRFEEELFLRSL a t g t g g a t t t c g a g t c g t c t t a a t g t a t a g a t t t g a a g a a g a g c t g t t t t t a c g a t c c c t t a c a c c t a a a g c t c a g c a g a a t t a c a t a t c t a a a c t t c t t c t c g a c a a a a a t g c t a g g g a

3480 280 285 290 295 QDYKIQSALLVPTLFSFFAK luciferase QDYKIQSALLVPTLFSFFAK t c a g g a t t a c a a a a t t c a a a g t g c g t t g c t a g t a c c a a c c c t a t t t t c a t t c t t c g c c a a a g t c c t a a t g t t t t a a g t t t c a c g c a a c g a t c a t g g t t g g g a t a a a a g t a a g a a g c g g t t

3540 300 305 310 315 STLIDKYDLSNLHEIASGGA luciferase STLIDKYDLSNLHEIASGGA a a g c a c t c t g a t t g a c a a a t a c g a t t t a t c t a a t t t a c a c g a a a t t g c t t c t g g g g g c g c t t c g t g a g a c t a a c t g t t t a t g c t a a a t a g a t t a a a t g t g c t t t a a c g a a g a c c c c c g c g

3600 320 325 330 335 PLSKEVGEAVAKRFHLPGIR luciferase PLSKEVGEAVAKRFHLPGIR a c c t c t t t c g a a a g a a g t c g g g g a a g c g g t t g c a a a a c g c t t c c a t c t t c c a g g g a t a c g t g g a g a a a g c t t t c t t c a g c c c c t t c g c c a a c g t t t t g c g a a g g t a g a a g g t c c c t a t g c

3660 340 345 350 355 QGYGLTETTSAILITPEGDD luciferase QGYGLTETTSAILITPEGDD a c a a g g a t a t g g g c t c a c t g a g a c t a c a t c a g c t a t t c t g a t t a c a c c c g a g g g g g a t g a t g t t c c t a t a c c c g a g t g a c t c t g a t g t a g t c g a t a a g a c t a a t g t g g g c t c c c c c t a c t

3720 360 365 370 375 KPGAVGKVVPFFEAKVVDLD luciferase KPGAVGKVVPFFEAKVVDLD t a a a c c g g g c g c g g t c g g t a a a g t t g t t c c a t t t t t t g a a g c g a a g g t t g t g g a t c t g g a a t t t g g c c c g c g c c a g c c a t t t c a a c a a g g t a a a a a a c t t c g c t t c c a a c a c c t a g a c c t

3780 380 385 390 395 TGKTLGVNQRGELCVRGPMI luciferase TGKTLGVNQRGELCVRGPMI t a c c g g g a a a a c g c t g g g c g t t a a t c a g a g a g g c g a a t t a t g t g t c a g a g g a c c t a t g a t a t g g c c c t t t t g c g a c c c g c a a t t a g t c t c t c c g c t t a a t a c a c a g t c t c c t g g a t a c t a

3840 400 405 410 415 MSGYVNNPEATNALIDKDGW luciferase MSGYVNNPEATNALIDKDGW t a t g t c c g g t t a t g t a a a c a a t c c g g a a g c g a c c a a c g c c t t g a t t g a c a a g g a t g g a t g a t a c a g g c c a a t a c a t t t g t t a g g c c t t c g c t g g t t g c g g a a c t a a c t g t t c c t a c c t a c

3900 420 425 430 435 LHSGDIAYWDEDEHFFIVDR luciferase LHSGDIAYWDEDEHFFIVDR g c t a c a t t c t g g a g a c a t a g c t t a c t g g g a c g a a g a c g a a c a c t t c t t c a t a g t t g a c c g c g a t g t a a g a c c t c t g t a t c g a a t g a c c c t g c t t c t g c t t g t g a a g a a g t a t c a a c t g g c

3960 440 445 450 455 LKSLIKYKGYQVAPAELESI luciferase LKSLIKYKGYQVAPAELESI c t t g a a g t c t t t a a t t a a a t a c a a a g g g t a t c a g g t g g c c c c c g c t g a a t t g g a a t c g a t g a a c t t c a g a a a t t a a t t t a t g t t t c c c a t a g t c c a c c g g g g g c g a c t t a a c c t t a g c t a

SgrAI 4020 460 465 470 475 LLQHPNIFDAGVAGLPDDDA luciferase LLQHPNIFDAGVAGLPDDDA a t t g t t a c a a c a c c c c a a c a t c t t c g a c g c g g g c g t g g c a g g t c t t c c c g a c g a t g a c g c t a a c a a t g t t g t g g g g t t g t a g a a g c t g c g c c c g c a c c g t c c a g a a g g g c t g c t a c t g c g

4080 480 485 490 495 GELPAAVVVLEHGKTMTEKE luciferase GELPAAVVVLEHGKTMTEKE c g g t g a a c t t c c c g c c g c c g t t g t t g t t t t g g a g c a c g g a a a g a c g a t g a c g g a a a a a g a g c c a c t t g a a g g g c g g c g g c a a c a a c a a a a c c t c g t g c c t t t c t g c t a c t g c c t t t t t c t

4140 500 505 510 515 IVDYVASQVTTAKKLRGGVV luciferase IVDYVASQVTTAKKLRGGVV g a t c g t g g a t t a c g t c g c c a g t c a a g t a a c a a c c g c g a a a a a g t t g c g c g g a g g a g t t g t c t a g c a c c t a a t g c a g c g g t c a g t t c a t t g t t g g c g c t t t t t c a a c g c g c c t c c t c a a c a

4200 520 525 530 535 FVDEVPKGLTGKLDARKIRE luciferase FVDEVPKGLTGKLDARKIRE g t t t g t g g a c g a a g t a c c g a a a g g t c t t a c c g g a a a a c t c g a c g c a a g a a a a a t c a g a g a c a a a c a c c t g c t t c a t g g c t t t c c a g a a t g g c c t t t t g a g c t g c g t t c t t t t t a g t c t c t

EcoRIEcoNI 4260 540 545 550 ILIKAKKGGKSKL* luciferase ILIKAKKGGKSKL* g a t c c t c a t a a a g g c c a a g a a g g g c g g a a a g t c c a a a t t g t a a g a a t t c g g g c g t g g c t g c t a g g a g t a t t t c c g g t t c t t c c c g c c t t t c a g g t t t a a c a t t c t t a a g c c c g c a c c g a c

4320 t t g g c c c a t a t g c a t a t a c a a t a c g a a g a g a g c c a g c a a t t a g g g t g t a a g g c a t t t a g a a a c c g g g t a t a c g t a t a t g t t a t g c t t c t c t c g g t c g t t a a t c c c a c a t t c c g t a a a t c t

4380 g g a a a c a t c a a c a g t a c t t t a a a a t t t a g c t t g a a a t a t g t t t a g c g a a a t g t a g c t c t t c c t t t g t a g t t g t c a t g a a a t t t t a a a t c g a a c t t t a t a c a a a t c g c t t t a c a t c g a g a a

4440 c c g g t c a g c c a g a g t a a c c g g a a a c g g a a a c g t a a a c g c c g g a c g c g c a t a c a a t a c t c a g g c c a g t c g g t c t c a t t g g c c t t t g c c t t t g c a t t t g c g g c c t g c g c g t a t g t t a t g a g t

4500 t c g a c a c a c t c a a g t t g c c a c t c g g a c g a a t g g a c t g t t g t t t a t t g t g c a a t t c g c a c g a g c t g t g t g a g t t c a a c g g t g a g c c t g c t t a c c t g a c a a c a a a t a a c a c g t t a a g c g t g c

4560 a g a c g a a c t c a c a g g c a t t g c a t t t t c a a g a a a t t a g t a t t a c a t a a t t g t a t c c g t a t c t c t g c t t g a g t g t c c g t a a c g t a a a a g t t c t t t a a t c a t a a t g t a t t a a c a t a g g c a t a g

4620 t g t t a a g t a c t c c g g g c g a a g a g a t c a a t t c a a a a t g c g g t t g t g t c g t c g a a a a a a a a a a c a a t t c a t g a g g c c c g c t t c t c t a g t t a a g t t t t a c g c c a a c a c a g c a g c t t t t t t t t t

4680 c a a a c t c g a a a a t a c t c a t g c a c a g a c t c t g a a t a a g t t c c c c t t t g c a g a a c g c a a t t t g t t t g a g c t t t t a t g a g t a c g t g t c t g a g a c t t a t t c a a g g g g a a a c g t c t t g c g t t a a a

4740 a c t c a t c a g t c a g t a t t c t c t a t g g c c a c t g t a t t a t t t t t a t a c c t t c a t t t g t t a g c t t g a g t a g t c a g t c a t a a g a g a t a c c g g t g a c a t a a t a a a a a t a t g g a a g t a a a c a a t c g a

4800 t t a a g t t t t t t t t a c a c a a c t a c t a c t t a t a c t t g t g c t g c c a c c a g t t g g g c t g c c g t a a a t t c a a a a a a a a t g t g t t g a t g a t g a a t a t g a a c a c g a c g g t g g t c a a c c c g a c g g c a t

4860 g t t g c a g a t c a a t g t a t c c a t t g t c c a t a c a t t g c g a g a a t c t t t t a t a a t t c g t c t a a g c a a c g t c t a g t t a c a t a g g t a a c a g g t a t g t a a c g c t c t t a g a a a a t a t t a a g c a g a t t c

PaqCI 4920 c a t a a g t t a c t g t a c g t c g t g t t g t a t a g c g c t g t a a t a t c c t g t c a c c t g c t t c t g c t t g t a t t c a a t g a c a t g c a g c a c a a c a t a t c g c g a c a t t a t a g g a c a g t g g a c g a a g a c g a a

4980 MKY *IPRSCPVILGNSLSIC a a t t a a t a c c c t a t a t c g g g c g a g a g c a a g g t a c g a t t a a a c c a t t a g a t a a t g a a a t a c t t a a t t a t g g g a t a t a g c c c g c t c t c g t t c c a t g c t a a t t t g g t a a t c t a t t a c t t t a t g

SalI 5040 KYIVYKQMKSTDALESLQPS MPLRAFNPV ICLTYVSSTSRHGQSGEVWD a a a t a c a t a g t g t a t a a a c a g a t g a a g t c g a c c g a t g c c c t t g a g a g c c t t c a a c c c a g t t t t a t g t a t c a c a t a t t t g t c t a c t t c a g c t g g c t a c g g g a a c t c t c g g a a g t t g g g t c a

PshAI 5100 QLLPVGAGHDYRRRTYDCLL SSFRWARGMTIVAALMTVFF AGEPPRPAHSDDGCKHSDEK c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t a t c g t c g c c g c a c t t a t g a c t g t c t t c t t g t c g a g g a a g g c c a c c c g c g c c c c g t a c t g a t a g c a g c g g c g t g a a t a c t g a c a g a a g a a

5160 YHATRRTGAGSALGHFRRGP IMQLVGQVPAALWVIFGEDR DHLEYSLHRCREPDNEALVA t a t c a t g c a a c t c g t a g g a c a g g t g c c g g c a g c g c t c t g g g t c a t t t t c g g c g a g g a c c g a t a g t a c g t t g a g c a t c c t g t c c a c g g c c g t c g c g a g a c c c a g t a a a a g c c g c t c c t g g c

5220 LSLERDDDRPVACGIRNLAR FRWSATMIGLSLAVFGILHA KAPARRHDAQRKRYESDQVG c t t t c g c t g g a g c g c g a c g a t g a t c g g c c t g t c g c t t g c g g t a t t c g g a a t c t t g c a c g c g a a a g c g a c c t c g c g c t g c t a c t a g c c g g a c a g c g a a c g c c a t a a g c c t t a g a a c g t g c g

5280 PRSSLRHWSRHQTFRREAGH LAQAFVTGPATKRFGEKQAI ESLGEDSTGGGFTEALLLGN c c t c g c t c a a g c c t t c g t c a c t g g t c c c g c c a c c a a a c g t t t c g g c g a g a a g c a g g c c a t g g a g c g a g t t c g g a a g c a g t g a c c a g g g c g g t g g t t t g c a a a g c c g c t c t t c g t c c g g t a

EagI 5340 YRRHGGRRAGLRLAGVRDAR IAGMAADALGYVLLAFATRG DGAHRGVRQAVDQQRERRSA t a t c g c c g g c a t g g c g g c c g a c g c g c t g g g c t a c g t c t t g c t g g c g t t c g c g a c g c g a g g a t a g c g g c c g t a c c g c c g g c t g c g c g a c c c g a t g c a g a a c g a c c g c a a g c g c t g c g c t c c

5400 LDGLPHYDSSRFRRHRDARV WMAFPIMILLASGGIGMPAL PHGEGNHNKESGAADPHGRQ c t g g a t g g c c t t c c c c a t t a t g a t t c t t c t c g c t t c c g g c g g c a t c g g g a t g c c c g c g t t g a c c t a c c g g a a g g g g t a a t a c t a a g a a g a g c g a a g g c c g c c g t a g c c c t a c g g g c g c a a

5460 AGHAVQAGR* QAMLSRQVDDDHQGQLQGSL LGHQGPLYIVVMLSLKLSRE g c a g g c c a t g c t g t c c a g g c a g g t a g a t g a c g a c c a t c a g g g a c a g c t t c a a g g a t c g c t c g t c c g g t a c g a c a g g t c c g t c c a t c t a c t g c t g g t a g t c c c t g t c g a a g t t c c t a g c g a

5520 AALTSQQKARNRKKAALLAF RSKGALLLGPVTFLGRQQRK c g c g g c t c t t a c c a g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g c g c c g a g a a t g g t c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a

DrdI 5580 ori FHRLRPPDEHHKNRRSSQRW EMPEAGRVLM t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c

5640 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F RNPTGL* g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c

5700 ori c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c

5760 ori c g t g g c g c t t t c t c a a t g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g

5820 ori c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t

5880 ori c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c

5940 ori t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g

6000 ori t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g

6060 ori c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c

6120 ori t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a

6180 g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a

6240 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t

6300 285 *WHKILS AmpR *WHKILS a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t

AhdI 6360 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a

6420 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c

6480 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t

NmeAIII 6540 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t

PstI 6600 200 195 190 185 ALTLLEGTLLKRLTTAMAAP AmpR ALTLLEGTLLKRLTTAMAAP a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t g c a g g t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a c g t c c

6660 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g

6720 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g

6780 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t

6840 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g

6900 100 95 90 85 LDNQSYHIRRGLQEQGADVR AmpR LDNQSYHIRRGLQEQGADVR c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a c a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t g t g c

6960 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g

7020 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c

7080 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g

7140 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR PLCFAAFFPILAVRFHQISM a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a

7200 AmpR promoter a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t

7260 AmpR promoter c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t

7320 a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c

7338 t a t c a c g a g c c c t t t c g t a t a g t g c t c g g g a a a g c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.