Addgene TFORF3206 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: TFORF3206


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2000 1500 1000 500 End (2255) SpeI (2170) BsaAI - SnaBI (2159) TaqII (2134) EcoRV (2115) SmlI (1999) BpuEI (1984) Esp3I BsmBI (1879) AfeI (1816) EcoNI (1786) NotI - SacII (1748) ScaI (1696) AscI (1469) SbfI (1337) NcoI (1316) BtgZI (1122) TsoI (1105) BspEI - BsaWI (906) NsiI (905) PflMI (827) SspI (792) SacI (600) Eco53kI (598) MmeI (548) BciVI (535) PflFI - Tth111I (304) AatII (272) ZraI (270) DrdI (222) BstXI (45) ClaI - BspDI (2) Start (0) attB1 attB2 V5 tag TFORF3206 2255 bp

Sequence View

BstXIStart (0) BspDIClaI 60 attB1 MQLEHCL a t c g a t t g a t c a a c a a g t t t g t a c a a a a a a g t t g g c a c c a t g c a g c t g g a g c a c t g c c t t t a g c t a a c t a g t t g t t c a a a c a t g t t t t t t c a a c c g t g g t a c g t c g a c c t c g t g a c g g a a

120 SPSIMLSKKFLNVSSSYPHS t c t c c t t c t a t c a t g c t c t c c a a g a a a t t t c t c a a t g t g a g c a g c a g c t a c c c a c a t t c a a g a g g a a g a t a g t a c g a g a g g t t c t t t a a a g a g t t a c a c t c g t c g t c g a t g g g t g t a a g t

180 GGSELVLHDHPIISTTDNLE g g c g g a t c c g a g c t t g t c t t g c a c g a t c a t c c c a t t a t c t c g a c c a c t g a c a a c c t g g a g c c g c c t a g g c t c g a a c a g a a c g t g c t a g t a g g g t a a t a g a g c t g g t g a c t g t t g g a c c t c

DrdI 240 RSSPLKKITRGMTNQSDTDN a g a a g t t c a c c t t t g a a a a a a a t t a c c a g g g g g a t g a c g a a t c a g t c a g a t a c a g a c a a t t c t t c a a g t g g a a a c t t t t t t t a a t g g t c c c c c t a c t g c t t a g t c a g t c t a t g t c t g t t a

ZraIAatII 300 FPDSKDSPGDVQRSKLSPVL t t t c c t g a c t c c a a g g a c t c a c c a g g g g a c g t c c a g a g a a g t a a a c t c t c t c c t g t c t t g a a a g g a c t g a g g t t c c t g a g t g g t c c c c t g c a g g t c t c t t c a t t t g a g a g a g g a c a g a a c

Tth111IPflFI 360 DGVSELRHSFDGSAADRYLL g a c g g g g t c t c t g a g c t t c g t c a c a g t t t c g a t g g c t c t g c t g c a g a t c g c t a c c t c c t c c t g c c c c a g a g a c t c g a a g c a g t g t c a a a g c t a c c g a g a c g a c g t c t a g c g a t g g a g g a g

420 SQSSQPQSAATAPSAMFPYP t c t c a g t c c a g c c a g c c a c a g t c t g c g g c c a c t g c t c c c a g t g c c a t g t t c c c g t a c c c c a g a g t c a g g t c g g t c g g t g t c a g a c g c c g g t g a c g a g g g t c a c g g t a c a a g g g c a t g g g g

480 GQHGPAHPAFSIGSPSRYMA g g c c a g c a c g g a c c g g c g c a c c c c g c c t t c t c c a t c g g c a g c c c t a g c c g c t a c a t g g c c c c g g t c g t g c c t g g c c g c g t g g g g c g g a a g a g g t a g c c g t c g g g a t c g g c g a t g t a c c g g

BciVI 540 HHPVITNGAYNSLLSNSSPQ c a c c a c c c g g t c a t c a c c a a c g g a g c c t a c a a c a g c c t c c t g t c c a a c t c c t c g c c g c a g g t g g t g g g c c a g t a g t g g t t g c c t c g g a t g t t g t c g g a g g a c a g g t t g a g g a g c g g c g t c

Eco53kISacIMmeI 600 GYPTAGYPYPQQYGHSYQGA g g a t a c c c c a c g g c c g g c t a c c c c t a c c c a c a g c a g t a c g g c c a c t c c t a c c a a g g a g c t c c t a t g g g g t g c c g g c c g a t g g g g a t g g g t g t c g t c a t g c c g g t g a g g a t g g t t c c t c g a

660 PFYQFSSTQPGLVPGKAQVY c c g t t c t a c c a g t t c t c c t c c a c c c a g c c g g g g c t g g t g c c c g g c a a a g c a c a g g t g t a c g g c a a g a t g g t c a a g a g g a g g t g g g t c g g c c c c g a c c a c g g g c c g t t t c g t g t c c a c a t g

720 LCNRPLWLKFHRHQTEMIIT c t g t g c a a c a g g c c c c t t t g g c t g a a a t t t c a c c g g c a c c a a a c g g a g a t g a t c a t c a c c g a c a c g t t g t c c g g g g a a a c c g a c t t t a a a g t g g c c g t g g t t t g c c t c t a c t a g t a g t g g

780 KQGRRMFPFLSFNISGLDPT a a a c a g g g a a g g c g c a t g t t t c c t t t t t t a a g t t t t a a c a t t t c t g g t c t c g a t c c c a c g t t t g t c c c t t c c g c g t a c a a a g g a a a a a a t t c a a a a t t g t a a a g a c c a g a g c t a g g g t g c

PflMISspI 840 AHYNIFVDVILADPNHWRFQ g c t c a t t a c a a t a t t t t t g t g g a t g t g a t t t t g g c g g a t c c c a a t c a c t g g a g g t t t c a a c g a g t a a t g t t a t a a a a a c a c c t a c a c t a a a a c c g c c t a g g g t t a g t g a c c t c c a a a g t t

900 GGKWVPCGKADTNVQGNRVY g g a g g c a a a t g g g t t c c t t g c g g c a a a g c g g a c a c c a a t g t g c a a g g a a a t c g g g t c t a t c c t c c g t t t a c c c a a g g a a c g c c g t t t c g c c t g t g g t t a c a c g t t c c t t t a g c c c a g a t a

NsiIBsaWIBspEI 960 MHPDSPNTGAHWMRQEISFG a t g c a t c c g g a t t c c c c c a a c a c t g g g g c t c a c t g g a t g c g c c a a g a a a t c t c t t t t g g a t a c g t a g g c c t a a g g g g g t t g t g a c c c c g a g t g a c c t a c g c g g t t c t t t a g a g a a a a c c t

1020 KLKLTNNKGASNNNGQMVVL * a a a t t a a a a c t t a c g a a c a a c a a a g g a g c t t c a a a t a a c a a t g g g c a g a t g g t g g t t t t a t t t a a t t t t g a a t g c t t g t t g t t t c c t c g a a g t t t a t t g t t a c c c g t c t a c c a c c a a a a t

1080 QSLHKYQPRLHVVEVNEDGT LGQVLVLGAQMHHFHVLVAR c a g t c c t t g c a c a a g t a c c a g c c c c g c c t g c a t g t g g t g g a a g t g a a c g a g g a c g g c a c g g t c a g g a a c g t g t t c a t g g t c g g g g c g g a c g t a c a c c a c c t t c a c t t g c t c c t g c c g t g c

BtgZITsoI 1140 EDTSQPGRVQTFTFPETQFI LVSALGAAHLRESERLSLED g a g g a c a c t a g c c a g c c c g g c c g c g t g c a g a c g t t c a c t t t c c c t g a g a c t c a g t t c a t c c t c c t g t g a t c g g t c g g g c c g g c g c a c g t c t g c a a g t g a a a g g g a c t c t g a g t c a a g t a g

1200 AVTAYQNTDITQLKIDHNPF GDGGVLVRINCLQFYIVVRK g c c g t c a c c g c c t a c c a g a a c a c g g a t a t t a c a c a a c t g a a a a t a g a t c a c a a c c c t t t t c g g c a g t g g c g g a t g g t c t t g t g c c t a t a a t g t g t t g a c t t t t a t c t a g t g t t g g g a a a a

1260 AKGFRDNYDTIYTGCDMDRL CFSKPIIIVRDVGATVHVAQ g c a a a a g g a t t t c g g g a t a a t t a t g a c a c g a t c t a c a c c g g c t g t g a c a t g g a c c g c c t g c g t t t t c c t a a a g c c c t a t t a a t a c t g t g c t a g a t g t g g c c g a c a c t g t a c c t g g c g g a c

NcoI 1320 TPSPNDSPRSQIVPGARYAM GGRGVVRRARLDHGPGAVGH a c c c c c t c g c c c a a c g a c t c g c c g c g c t c g c a g a t c g t g c c c g g g g c c c g c t a c g c c a t g t g g g g g a g c g g g t t g c t g a g c g g c g c g a g c g t c t a g c a c g g g c c c c g g g c g a t g c g g t a c

SbfI 1380 AGSFLQDQFVSNYAKARFHP GAREQLVLEHAVVGLGAEVR g c c g g c t c t t t c c t g c a g g a c c a g t t c g t g a g c a a c t a c g c c a a g g c c c g c t t c c a c c c g c g g c c g a g a a a g g a c g t c c t g g t c a a g c a c t c g t t g a t g c g g t t c c g g g c g a a g g t g g g c

1440 GAGAGPGPGTDRSVPHTNGL ARARAGPRTRVAAHRVGVPQ g g c g c g g g c g c g g g c c c c g g g c c g g g t a c g g a c c g c a g c g t g c c g c a c a c c a a c g g g c t g c c g c g c c c g c g c c c g g g g c c c g g c c c a t g c c t g g c g t c g c a c g g c g t g t g g t t g c c c g a c

AscI 1500 LSPQQAEDPGAPSPQRWFVT QRRLLGLVRARGRRLAPKHR c t g t c g c c g c a g c a g g c c g a g g a c c c g g g c g c g c c c t c g c c g c a a c g c t g g t t t g t g a c g g a c a g c g g c g t c g t c c g g c t c c t g g g c c c g c g c g g g a g c g g c g t t g c g a c c a a a c a c t g c

1560 PANNRLDFAASAYDTATDFA RGVVPQVERGRGIVRGRVER c c g g c c a a c a a c c g g c t g g a c t t c g c g g c c t c g g c c t a t g a c a c g g c c a c g g a c t t c g c g g g c c g g t t g t t g g c c g a c c t g a a g c g c c g g a g c c g g a t a c t g t g c c g g t g c c t g a a g c g c

1620 GNAATLLSYAAAGVKALPLQ AVRGRQERVRRRAHLRQRQL g g c a a c g c g g c c a c g c t g c t c t c t t a c g c g g c g g c g g g c g t g a a g g c g c t g c c g c t g c a g c c g t t g c g c c g g t g c g a c g a g a g a a t g c g c c g c c g c c c g c a c t t c c g c g a c g g c g a c g t c

1680 AAGCTGRPLGYYADPSGWGA SCAASAAREAVVGVRRAPAG g c t g c a g g c t g c a c t g g c c g c c c g c t c g g c t a c t a c g c c g a c c c g t c g g g c t g g g g c g c c c g a c g t c c g a c g t g a c c g g c g g g c g a g c c g a t g a t g c g g c t g g g c a g c c c g a c c c c g c g g

ScaI 1740 RSPPQYCGTKSGSVLPCWPN ATGRLVAAGLRARHQGAPGV c g c a g t c c c c c g c a g t a c t g c g g c a c c a a g t c g g g c t c g g t g c t g c c c t g c t g g c c c a a c g c g t c a g g g g g c g t c a t g a c g c c g t g g t t c a g c c c g a g c c a c g a c g g g a c g a c c g g g t t g

EcoNISacIINotI 1800 SAAAAARMAGANPYLGEEAE AGRGGRAHGAGIGVQALLGL a g c g c c g c g g c c g c c g c g c g c a t g g c c g g c g c c a a t c c c t a c c t g g g c g a g g a g g c c g a g t c g c g g c g c c g g c g g c g c g c g t a c c g g c c g c g g t t a g g g a t g g a c c c g c t c c t c c g g c t c

AfeI 1860 GLAAERSPLPPGAAEDAKPK AQGGLARRQRGAGGLVGLGL g g c c t g g c c g c c g a g c g c t c g c c g c t g c c g c c c g g c g c c g c c g a g g a c g c c a a g c c c a a g c c g g a c c g g c g g c t c g c g a g c g g c g a c g g c g g g c c g c g g c g g c t c c t g c g g t t c g g g t t c

BsmBIEsp3I 1920 DLSDSSWIETPSSIKSIDSS VQGIGAPDLRGGRDLGDVGA g a c c t g t c c g a t t c c a g c t g g a t c g a g a c g c c c t c c t c g a t c a a g t c c a t c g a c t c c a g c c t g g a c a g g c t a a g g t c g a c c t a g c t c t g c g g g a g g a g c t a g t t c a g g t a g c t g a g g t c g

1980 DSGIYEQAKRRRISPADTPV VRPNVLLGLPPPDRRGVRGH g a c t c g g g g a t t t a c g a g c a g g c c a a g c g g a g g c g g a t c t c g c c g g c c g a c a c g c c c g t g c t g a g c c c c t a a a t g c t c g t c c g g t t c g c c t c c g c c t a g a g c g g c c g g c t g t g c g g g c a c

SmlIBpuEI 2040 SESSSPLKSEVLAQRDCEKN GLTRGRELALHQGLPVALLV t c c g a g a g t t c g t c c c c g c t c a a g a g c g a g g t g c t g g c c c a g c g g g a c t g c g a g a a g a a c a g g c t c t c a a g c a g g g g c g a g t t c t c g c t c c a c g a c c g g g t c g c c c t g a c g c t c t t c t t g

2100 attB2 CAKDISGYYGFYSHSLPTFL AGLVNAAVIAEVRVAQWSEQ t g c g c c a a g g a c a t t a g c g g c t a c t a t g g c t t c t a c t c g c a c a g c t t g c c a a c t t t c t t g a c g c g g t t c c t g t a a t c g c c g a t g a t a c c g a a g a t g a g c g t g t c g a a c g g t t g a a a g a a c

SnaBIBsaAITaqIIEcoRV 2160 1 5 10 GKPIPNPLLGLDST attB2 V5 tag YKVVDIGKPIPNPLLGLDST VFHNIDTLRDRVREETEIRR t a c a a a g t g g t t g a t a t c g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g a t g t t t c a c c a a c t a t a g c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c

SpeI 2220 ** LLSSTRLKFTNRNRPKIYRT t a g t a a t g a a c t a g t c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t a t c a t t a c t t g a t c a g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a

End (2255) 2255 SLVVYDQFNSK g g a a a g g a c g a c g t a a t c c t g a a a a t t a g a t t t t a c c t t t c c t g c t g c a t t a g g a c t t t t a a t c t a a a a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.