Addgene TFORF3066 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: TFORF3066


Map View

2000 1500 1000 500 End (2186) SpeI (2101) SnaBI (2090) BsaI (2083) TaqII (2065) EcoRV (2046) BsiEI (2013) MslI (1921) FspI - FspAI (1915) SacI (1772) Eco53kI (1770) KflI (1710) AatII - XcmI (1588) ZraI (1586) AfeI (1558) PmlI (1534) AflIII (1533) BglII (1496) BstAPI (1443) BpuEI (1429) SmlI (1408) NmeAIII (1374) SapI - BspQI (1310) PflMI * (1173) BmrI (1161) EcoNI (907) BamHI (894) Bpu10I (863) Bsu36I (845) SfiI - BglI (744) ScaI (688) PshAI (578) StuI (369) BspEI * (200) BspHI * (152) BclI * (7) ClaI - BspDI (2) Start (0) attB1 attB2 V5 tag TFORF3066 2186 bp

Sequence View

BclI*Start (0) BspDIClaI 60 attB1 MEGDGSDP a t c g a t t g a t c a a c a a g t t t g t a c a a a a a a g t t g g c a t g g a g g g a g a t g g t t c a g a c c c a t a g c t a a c t a g t t g t t c a a a c a t g t t t t t t c a a c c g t a c c t c c c t c t a c c a a g t c t g g g t

120 EPPDAGEDSKSENGENAPIY g a g c c t c c a g a t g c c g g g g a g g a c a g c a a g t c c g a g a a t g g g g a g a a t g c g c c c a t c t a c c t c g g a g g t c t a c g g c c c c t c c t g t c g t t c a g g c t c t t a c c c c t c t t a c g c g g g t a g a t g

BspHI* 180 CICRKPDINCFMIGCDNCNE t g c a t c t g c c g c a a a c c g g a c a t c a a c t g c t t c a t g a t c g g g t g t g a c a a c t g c a a t g a g a c g t a g a c g g c g t t t g g c c t g t a g t t g a c g a a g t a c t a g c c c a c a c t g t t g a c g t t a c t c

BspEI* 240 WFHGDCIRITEKMAKAIREW t g g t t c c a t g g g g a c t g c a t c c g g a t c a c t g a g a a g a t g g c c a a g g c c a t c c g g g a g t g g a c c a a g g t a c c c c t g a c g t a g g c c t a g t g a c t c t t c t a c c g g t t c c g g t a g g c c c t c a c c

300 YCRECREKDPKLEIRYRHKK t a c t g t c g g g a g t g c a g a g a g a a a g a c c c c a a g c t a g a g a t t c g c t a t c g g c a c a a g a a a a t g a c a g c c c t c a c g t c t c t c t t t c t g g g g t t c g a t c t c t a a g c g a t a g c c g t g t t c t t t

360 SRERDGNERDSSEPRDEGGG t c a c g g g a g c g g g a t g g c a a t g a g c g g g a c a g c a g t g a g c c c c g g g a t g a g g g t g g a g g g a g t g c c c t c g c c c t a c c g t t a c t c g c c c t g t c g t c a c t c g g g g c c c t a c t c c c a c c t c c c

StuI 420 RKRPVPDPDLQRRAGSGTGV c g c a a g a g g c c t g t c c c t g a t c c a g a c c t g c a g c g c c g g g c a g g g t c a g g g a c a g g g g t t g c g t t c t c c g g a c a g g g a c t a g g t c t g g a c g t c g c g g c c c g t c c c a g t c c c t g t c c c c a a

480 GAMLARGSASPHKSSPQPLV g g g g c c a t g c t t g c t c g g g g c t c t g c t t c g c c c c a c a a a t c c t c t c c g c a g c c c t t g g t g c c c c g g t a c g a a c g a g c c c c g a g a c g a a g c g g g g t g t t t a g g a g a g g c g t c g g g a a c c a c

540 ATPSQHHQQQQQQIKRSARM g c c a c a c c c a g c c a g c a t c a c c a g c a g c a g c a g c a g c a g a t c a a a c g g t c a g c c c g c a t g c g g t g t g g g t c g g t c g t a g t g g t c g t c g t c g t c g t c g t c t a g t t t g c c a g t c g g g c g t a c

PshAI 600 CGECEACRRTEDCGHCDFCR t g t g g t g a g t g t g a g g c a t g t c g g c g c a c t g a g g a c t g t g g t c a c t g t g a t t t c t g t c g g a c a c c a c t c a c a c t c c g t a c a g c c g c g t g a c t c c t g a c a c c a g t g a c a c t a a a g a c a g c c

660 DMKKFGGPNKIRQKCRLRQC g a c a t g a a g a a g t t c g g g g g c c c c a a c a a g a t c c g g c a g a a g t g c c g g c t g c g c c a g t g c c t g t a c t t c t t c a a g c c c c c g g g g t t g t t c t a g g c c g t c t t c a c g g c c g a c g c g g t c a c g

ScaI 720 QLRARESYKYFPSSLSPVTP c a g c t g c g g g c c c g g g a a t c g t a c a a g t a c t t c c c t t c c t c g c t c t c a c c a g t g a c g c c c g t c g a c g c c c g g g c c c t t a g c a t g t t c a t g a a g g g a a g g a g c g a g a g t g g t c a c t g c g g g

BglISfiI 780 SESLPRPRRPLPTQQQPQPS t c a g a g t c c c t g c c a a g g c c c c g c c g g c c a c t g c c c a c c c a a c a g c a g c c a c a g c c a t c a a g t c t c a g g g a c g g t t c c g g g g c g g c c g g t g a c g g g t g g g t t g t c g t c g g t g t c g g t a g t

840 QKLGRIREDEGAVASSTVKE c a g a a g t t a g g g c g c a t c c g t g a a g a t g a g g g g g c a g t g g c g t c a t c a a c a g t c a a g g a g g t c t t c a a t c c c g c g t a g g c a c t t c t a c t c c c c c g t c a c c g c a g t a g t t g t c a g t t c c t c

BamHIBpu10IBsu36I 900 PPEATATPEPLSDEDLPLDP c c t c c t g a g g c t a c a g c c a c a c c t g a g c c a c t c t c a g a t g a g g a c c t a c c t c t g g a t c c t g g a g g a c t c c g a t g t c g g t g t g g a c t c g g t g a g a g t c t a c t c c t g g a t g g a g a c c t a g g a

EcoNI 960 DLYQDFCAGAFDDHGLPWMS g a c c t g t a t c a g g a c t t c t g t g c a g g g g c c t t t g a t g a c c a t g g c c t g c c c t g g a t g a g c c t g g a c a t a g t c c t g a a g a c a c g t c c c c g g a a a c t a c t g g t a c c g g a c g g g a c c t a c t c g

1020 DTEESPFLDPALRKRAVKVK g a c a c a g a a g a g t c c c c a t t c c t g g a c c c c g c g c t g c g g a a g a g g g c a g t g a a a g t g a a g c t g t g t c t t c t c a g g g g t a a g g a c c t g g g g c g c g a c g c c t t c t c c c g t c a c t t t c a c t t c

1080 HVKRREKKSEKKVMERKEER c a t g t g a a g c g t c g g g a g a a g a a g t c t g a g a a g a a g g t g a t g g a g a g g a a g g a g g a g c g a g t a c a c t t c g c a g c c c t c t t c t t c a g a c t c t t c t t c c a c t a c c t c t c c t t c c t c c t c g c t

1140 YKRHRQKQKHKDKWKHPERA t a c a a g c g g c a t c g g c a g a a g c a g a a g c a c a a g g a t a a a t g g a a a c a c c c a g a g a g g g c t a t g t t c g c c g t a g c c g t c t t c g t c t t c g t g t t c c t a t t t a c c t t t g t g g g t c t c t c c c g a

PflMI*BmrI 1200 DAKDPASLPQCLGPGCVRPA g a t g c c a a g g a c c c t g c g t c a c t g c c c c a g t g c c t g g g g c c c g g c t g t g t g c g c c c c g c c c t a c g g t t c c t g g g a c g c a g t g a c g g g g t c a c g g a c c c c g g g c c g a c a c a c g c g g g g c g g

1260 QPSSKYCSDDCGMKLAANRI c a g c c c a g c t c c a a g t a t t g c t c a g a t g a c t g t g g c a t g a a g c t g g c a g c c a a c c g c a t c g t c g g g t c g a g g t t c a t a a c g a g t c t a c t g a c a c c g t a c t t c g a c c g t c g g t t g g c g t a g

BspQISapI 1320 YEILPQRIQQWQQSPCIAEE t a c g a g a t c c t c c c c c a g c g c a t c c a g c a g t g g c a g c a g a g c c c t t g c a t t g c t g a a g a g a t g c t c t a g g a g g g g g t c g c g t a g g t c g t c a c c g t c g t c t c g g g a a c g t a a c g a c t t c t c

NmeAIII 1380 HGKKLLERIRREQQSARTRL c a c g g c a a g a a g c t g c t c g a a c g c a t t c g c c g a g a g c a g c a g a g t g c c c g c a c c c g c c t t g t g c c g t t c t t c g a c g a g c t t g c g t a a g c g g c t c t c g t c g t c t c a c g g g c g t g g g c g g a a

BpuEISmlI 1440 QEMERRFHELEAIILRAKQQ MSLRPSFYVPSSR c a g g a a a t g g a a c g c c g a t t c c a t g a g c t t g a g g c c a t c a t t c t a c g t g c c a a g c a g c a g g t c c t t t a c c t t g c g g c t a a g g t a c t c g a a c t c c g g t a g t a a g a t g c a c g g t t c g t c g t c

BglIIBstAPI 1500 AVREDEESNEGDSDDTDLQI LCARMRRATRVTVMTQTCRS g c t g t g c g c g a g g a t g a g g a g a g c a a c g a g g g t g a c a g t g a t g a c a c a g a c c t g c a g a t c c g a c a c g c g c t c c t a c t c c t c t c g t t g c t c c c a c t g t c a c t a c t g t g t c t g g a c g t c t a g

AfeIAflIIIPmlI 1560 FCVSCGHPINPRVALRHMER SVFPVGTPSTHVLPCATWSA t t c t g t g t t t c c t g t g g g c a c c c c a t c a a c c c a c g t g t t g c c t t g c g c c a c a t g g a g c g c a a g a c a c a a a g g a c a c c c g t g g g g t a g t t g g g t g c a c a a c g g a a c g c g g t g t a c c t c g c g

ZraIXcmIAatII 1620 CYAKYESQTSFGSMYPTRIE ATPSMRARRPLGPCTPHALK t g c t a c g c c a a g t a t g a g a g c c a g a c g t c c t t t g g g t c c a t g t a c c c c a c a c g c a t t g a a a c g a t g c g g t t c a t a c t c t c g g t c t g c a g g a a a c c c a g g t a c a t g g g g t g t g c g t a a c t t

1680 GATRLFCDVYNPQSKTYCKR GPHDSSVMCIILRAKHTVSG g g g g c c a c a c g a c t c t t c t g t g a t g t g t a t a a t c c t c a g a g c a a a a c a t a c t g t a a g c g g c c c c g g t g t g c t g a g a a g a c a c t a c a c a t a t t a g g a g t c t c g t t t t g t a t g a c a t t c g c c

KflI 1740 LQVLCPEHSRDPKVPADEVC SRCCAPSTHGTPKCQLTRYA *PVGFHWSVLYA c t c c a g g t g c t g t g c c c c g a g c a c t c a c g g g a c c c c a a a g t g c c a g c t g a c g a g g t a t g c g a g g t c c a c g a c a c g g g g c t c g t g a g t g c c c t g g g g t t t c a c g g t c g a c t g c t c c a t a c g

Eco53kISacI 1800 GCPLVRDVFELTGDFCRLPK GAPLYVMSLSSRVTSAACPS PAGKYTIDKLERTVEAAQGL g g g t g c c c c c t t g t a c g t g a t g t c t t t g a g c t c a c g g g t g a c t t c t g c c g c c t g c c c a a g c c c a c g g g g g a a c a t g c a c t a c a g a a a c t c g a g t g c c c a c t g a a g a c g g c g g a c g g g t t c

1860 RQCNRHYCWEKLRRAEVDLE ASAIAITAGRSCGVRKWTWS ALAIAMVAPLLQPTRFHVQL c g c c a g t g c a a t c g c c a t t a c t g c t g g g a g a a g c t g c g g c g t g c g g a a g t g g a c t t g g a g g c g g t c a c g t t a g c g g t a a t g a c g a c c c t c t t c g a c g c c g c a c g c c t t c a c c t g a a c c t c

FspAIFspI 1920 RVRVWYKLDELFEQERNVRT ACVCGTSWTSCLSRSAMCAQ AHTHPVLQVLQKLLLAIHAC c g c g t g c g t g t g t g g t a c a a g c t g g a c g a g c t g t t t g a g c a g g a g c g c a a t g t g c g c a c a g c g c a c g c a c a c a c c a t g t t c g a c c t g c t c g a c a a a c t c g t c c t c g c g t t a c a c g c g t g t

MslI 1980 AMTNRAGLLALMLHQTIQHD P* GHCVARSQQGQHQVLRDLVI g c c a t g a c a a a c c g c g c g g g a t t g c t g g c c c t g a t g c t g c a c c a g a c g a t c c a g c a c g a t c g g t a c t g t t t g g c g c g c c c t a a c g a c c g g g a c t a c g a c g t g g t c t g c t a g g t c g t g c t a

BsiEI 2040 attB2 PLTTDLRSSADRCPTFLYKV GESGVQAGTGVAAWSEQVFH c c c c t c a c t a c c g a c c t g c g c t c c a g t g c c g a c c g c t g c c c a a c t t t c t t g t a c a a a g t g g g g g a g t g a t g g c t g g a c g c g a g g t c a c g g c t g g c g a c g g g t t g a a a g a a c a t g t t t c a c

SnaBIBsaITaqIIEcoRV 2100 1 5 10 GKPIPNPLLGLDST * attB2 V5 tag VDIGKPIPNPLLGLDST* NIDTLRDRVREETEIRRLLS g t t g a t a t c g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g t a g t a a t g a c a a c t a t a g c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c a t c a t t a c t

SpeI 2160 STRLKFTNRNRPKIYRTSLV a c t a g t c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c t g a t c a g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g

End (2186) 2186 FMVVASLV g a a c a t t a c a a c a g c t g a c a a c a c t c c t t g t a a t g t t g t c g a c t g t t g t g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.