Addgene Flag-HA-LOC220594 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Flag-HA-LOC220594 Sequencing Result

Map View

800 600 400 200 End (888) BpuEI (851) NdeI (844) BbvCI (836) PvuII - MspA1I (655) AfeI (596) NspI (566) BsgI (558) BstXI (509) SacI - BanII - BsiHKAI - Bsp1286I (493) Eco53kI (491) PasI (479) BssSI - BssSαI (439) KpnI (438) BsaI (436) Acc65I (434) ApoI (396) BclI * (365) AflII (342) BtsI BtsαI (280) TatI BsrGI (232) XhoI PspXI PaeR7I (211) BciVI (174) StyI - NcoI (142) PluTI (124) SfoI (122) NarI - BsaHI (121) KasI (120) EcoNI (115) pBABE 5' (93 .. 109) pLXSN 5' (55 .. 77) Esp3I - BsmBI (52) Start (0) FLAG HA attB1 Kozak sequence Flag-HA-LOC220594 888 bp

Sequence View

BsmBIEsp3IStart (0) 5ʹ 3ʹ 60 C C C T T G pLXSN 5' FVXPKPPPPLPPSAPSLPL c c t t t g t a c n c c c t a a g c c t c c g c c t c c t c t t c c t c c a t c c g c c c c g t c t c t c c c c c t t g g g a a a c a t g n g g g a t t c g g a g g c g g a g g a g a a g g a g g t a g g c g g g g c a g a g a g g g g g a a c

EcoNI 120 A A C C T C C T C G T T C G A C C pLXSN 5' C T T T A T C C A G C C C T C A C pBABE 5' EPPRSTPPRSSLYPALTPSL a a c c t c c t c g t t c g a c c c c g c c t c g a t c c t c c c t t t a t c c a g c c c t c a c t c c t t c t c t a g t t g g a g g a g c a a g c t g g g g c g g a g c t a g g a g g g a a a t a g g t c g g g a g t g a g g a a g a g a t c

BciVINcoIStyIKasIBsaHINarISfoIPluTI 180 1 5 DYKDDDDK LDG FLAG Kozak sequence GAGIRSATMDYKDDDDKLDG g c g c c g g a a t t a g a t c t g c c a c c a t g g a c t a c a a g g a t g a c g a t g a c a a g c t c g a t g g a g c g c g g c c t t a a t c t a g a c g g t g g t a c c t g a t g t t c c t a c t g c t a c t g t t c g a g c t a c c t c

BsrGITatIPaeR7IPspXIXhoI 240 G 1 5 YPYDVPDYA (in frame with HA) ARGYQTSLYK HA attB1 GYPYDVPDYAARGYQTSLYK g a t a c c c a t a c g a t g t t c c a g a t t a c g c t g c t c g a g g t t a t c a a a c a a g t t t g t a c a a a a c t a t g g g t a t g c t a c a a g g t c t a a t g c g a c g a g c t c c a a t a g t t t g t t c a a a c a t g t t t t

BtsαIBtsI 300 (in frame with HA) KVGMRYTTVPSVRPTALATK attB1 KVGMRYTTVPSVRPTALATK a a g t t g g c a t g a g a t a t a c t a c t g t t c c a a g t g t a a g a c c c a c t g c t t t a g c a a c a a a g a t t c a a c c g t a c t c t a t a t g a t g a c a a g g t t c a c a t t c t g g g t g a c g a a a t c g t t g t t t c t

AflII 360 (in frame with HA) KLDLWRLPPFLIIHLKRFQF KLDLWRLPPFLIIHLKRFQF a g c t g g a t c t c t g g a g g c t t c c a c c c t t c c t g a t t a t t c a c c t t a a g c g a t t t c a a t t t g t c g a c c t a g a g a c c t c c g a a g g t g g g a a g g a c t a a t a a g t g g a a t t c g c t a a a g t t a a a c

ApoIBclI* 420 (in frame with HA) VNDQWIKSQKIVKFPRESFD VNDQWIKSQKIVKFPRESFD t a a a t g a t c a g t g g a t a a a a t c a c a g a a a a t t g t c a a a t t t c c t c g g g a a a g t t t t g a t c a t t t a c t a g t c a c c t a t t t t a g t g t c t t t t a a c a g t t t a a a g g a g c c c t t t c a a a a c t a g

PasIAcc65IBsaIKpnIBssSαIBssSI 480 (in frame with HA) PSAFLVPRDPALCQHQPLTP PSAFLVPRDPALCQHQPLTP c g a g t g c t t t t t t g g t a c c a c g a g a c c c g g c t c t c t g c c a g c a t c a a c c a c t c a c a c c c c g c t c a c g a a a a a a c c a t g g t g c t c t g g g c c g a g a g a c g g t c g t a g t t g g t g a g t g t g g g g

BstXIEco53kIBsp1286IBsiHKAIBanIISacI 540 (in frame with HA) QGDELSKPRILAREVKKVDV QGDELSKPRILAREVKKVDV a g g g g g a t g a g c t c t c c a a g c c c a g g a t t c t g g c a a g a g a g g t g a a g a a a g t g g a t g t g c t c c c c c t a c t c g a g a g g t t c g g g t c c t a a g a c c g t t c t c t c c a c t t c t t t c a c c t a c a c g

AfeINspIBsgI 600 (in frame with HA) QSLAGEEDMLLSKSPSSLSA QSLAGEEDMLLSKSPSSLSA a g a g t t t g g c t g g g g a a g a g g a c a t g c t c c t g a g c a a a a g c c c a t c c t c a c t c a g c g c t a t c t c a a a c c g a c c c c t t c t c c t g t a c g a g g a c t c g t t t t c g g g t a g g a g t g a g t c g c g a t

MspA1IPvuII 660 (in frame with HA) NISSSPKGSPSSSRKSGTSC NISSSPKGSPSSSRKSGTSC a c a t c a g c a g c a g c c c a a a a g g t t c t c c t t c t t c a t c a a g a a a a a g t g g a a c c a g c t g t c t g t a g t c g t c g t c g g g t t t t c c a a g a g g a a g a a g t a g t t c t t t t t c a c c t t g g t c g a c a g

720 (in frame with HA) PSSKNSSPNSCPRTLGRSKG PSSKNSSPNSCPRTLGRSKG c c t c c a g c a a a a a c a g c a g c c c t a a t a g c t g c c c a c g g a c t t t g g g g a g g a g c a a a g g g a g g a g g t c g t t t t t g t c g t c g g g a t t a t c g a c g g g t g c c t g a a a c c c c t c c t c g t t t c c c t

780 (in frame with HA) RLXLPQIGSKNKLSSSKXNL RLXLPQIGSKNKLSSSKXNL g g c t c c n g c t g c c c c a g a t t g g c a g c a a a a a t a a a c t g t c a a g t a g t a a g a a n a a c t t g g c c g a g g n c g a c g g g g t c t a a c c g t c g t t t t t a t t t g a c a g t t c a t c a t t c t t n t t g a a c c

BbvCI 840 (in frame with HA) DASKXNGAGQICXLAXXLS* DASKXNGAGQICXLAXXLS* a t g c c a g c a a a g a n a a t g g g g c t g g g c a g a t c t g t g a n c t g g c t g a n g n c t t g a g c t g a g t a c g g t c g t t t c t n t t a c c c c g a c c c g t c t a g a c a c t n g a c c g a c t n c n g a a c t c g a c t c

End (888) BpuEINdeI 3ʹ 5ʹ 888 g g c a t a t g c g g g g g g g c a g c c a a c c a g a n c t g g n c a c t c c t c n n n a c c c c g t a t a c g c c c c c c c g t c g g t t g g t c t n g a c c n g t g a g g a g n n n t g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.