Addgene pRRL-GFPGO2(NGC)-PGK-Neo Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRRL-GFPGO2(NGC)-PGK-Neo Sequencing Result

Map View

T3 (8761 .. 8781) M13 rev M13 Reverse (8726 .. 8742) lac operator M13/pUC Reverse (8707 .. 8729) lac promoter CAP binding site L4440 (8494 .. 8511) PciI (8396) pBR322ori-F (8241 .. 8260) AhdI (7508) ScaI (7027) Amp-R (6939 .. 6958) F1ori-F (6432 .. 6453) DraIII (6368) F1ori-R (6222 .. 6241) M13/pUC Forward (5986 .. 6008) M13 Forward (5977 .. 5994) M13 fwd T7 (5948 .. 5967) T7 promoter AvrII (5927) SfiI (5880) EBV-rev (5723 .. 5742) SV40pA-R (5669 .. 5688) KpnI (5260) Acc65I (5256) Bsu36I (5252) SacII (5035) PflMI (4773) WPRE-R (4585 .. 4605) BstBI (4514) SalI (4507) BamHI (4501) T3 promoter NruI * (611) NotI (922) EcoNI (944) BstAPI (1227) KflI (1708) HpaI (1802) BspDI * - ClaI * (1925) NheI (1951) BmtI (1955) EcoRV (2063) TspMI - XmaI (2091) SmaI (2093) BstEII (2110) AscI (2334) EcoRI (2353) EGFP-N (2473 .. 2494) EXFP-R (2734 .. 2753) EGFP-C (3081 .. 3102) BsrGI (3137) NsiI (3158) MSCV-rev (3217 .. 3240) AgeI (3292) BsmBI - Esp3I - SpeI (3427) BlpI (3455) BspEI (3601) mPGK-F (3614 .. 3633) Neo-R (3758 .. 3777) MscI (3914) PflFI - Tth111I (3950) RsrII (4348) Neo-F (4368 .. 4387) pRRL-GFPGO2(NGC)-PGK-Neo 8804 bp

Sequence View

5ʹ 3ʹ 60 RSV promoter t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t

120 RSV promoter c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g

180 RSV promoter g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t

240 RSV promoter 5' LTR (truncated) t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g

300 5' LTR (truncated) t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c

360 5' LTR (truncated) c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a

420 5' LTR (truncated) g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g

480 HIV-1 Ψ c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g

540 HIV-1 Ψ t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a

600 HIV-1 Ψ t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t

NruI* 660 g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a

720 t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a

780 t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c

840 g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t

900 g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t

EcoNINotI 960 MR g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c

1020 DNWRSELYKYKVVKIEPLGV g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t

1080 RRE APTKAKRRVVQREKRAVGIG g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t

1140 RRE ALFLGFLGAAGSTMGAASMT g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c

1200 RRE LTVQARQLLSGIVQQQNNLL c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c

BstAPI 1260 RRE RAIEAQQHLLQLTVWGIKQL a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g

1320 RRE QARILAVERYLKDQQLLGIW c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c

1380 GCSGKLICTTAVPWNASWSN g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a

1440 KSLEQIWNHTTWMEWDREIN a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g

1500 1 KN gp41 peptide NYTSLIHSLIEESQNQQEKN a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a

1560 5 10 15 EQELLELDKWASL (in frame with gp41 peptide) WNWFNIT gp41 peptide EQELLELDKWASLWNWFNIT g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t

1620 (in frame with gp41 peptide) NWLWYIKLFIMIVGGLVGLR NWLWYIKLFIMIVGGLVGLR a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t

1680 (in frame with gp41 peptide) IVFAVLSIVNRVRQGYSPLS IVFAVLSIVNRVRQGYSPLS a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c

KflI 1740 (in frame with gp41 peptide) FQTHLPTPRGPDRPEGIEEE FQTHLPTPRGPDRPEGIEEE t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t

1800 (in frame with gp41 peptide) GGERDRDRSIRLVNGSRRYR GGERDRDRSIRLVNGSRRYR g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g g c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c c

HpaI 1860 (in frame with gp41 peptide) LTFKRKGGIGGYSAGERIVD cPPT/CTS LTFKRKGGIGGYSAGERIVD t t a a c t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a a t t g a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g

1920 (in frame with gp41 peptide) IIATDIQTKELQKQITKIQN cPPT/CTS IIATDIQTKELQKQITKIQN a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a

BmtINheIBspDI*ClaI* 1980 (in frame with gp41 peptide) FIDHETSLDRASCSNAILQG cPPT/CTS FIDHETSLDRASCSNAILQG t t t a t c g a t c a c g a g a c t a g c c t c g a t c g a g c t a g c t g c a g t a a c g c c a t t t t g c a a g g c a a a t a g c t a g t g c t c t g a t c g g a g c t a g c t c g a t c g a c g t c a t t g c g g t a a a a c g t t c c g

2040 (in frame with gp41 peptide) MEKYQTKNREVQIKGGYMKI MEKYQTKNREVQIKGGYMKI a t g g a a a a a t a c c a a a c c a a g a a t a g a g a a g t t c a g a t c a a g g g c g g g t a c a t g a a a a t a t a c c t t t t t a t g g t t t g g t t c t t a t c t c t t c a a g t c t a g t t c c c g c c c a t g t a c t t t t a t

TspMIXmaISmaIEcoRV 2100 (in frame with gp41 peptide) ANVGPNRISAVSSFGPGPGP ANVGPNRISAVSSFGPGPGP g c t a a c g t t g g g c c a a a c a g g a t a t c t g c g g t g a g c a g t t t c g g c c c c g g c c c g g g g c c a c g a t t g c a a c c c g g t t t g t c c t a t a g a c g c c a c t c g t c a a a g c c g g g g c c g g g c c c c g g t

BstEII 2160 (in frame with gp41 peptide) RTDGHRSFGPGPRPRTDGPQ RTDGHRSFGPGPRPRTDGPQ a g a a c a g a t g g t c a c c g c a g t t t c g g c c c c g g c c c g a g g c c a a g a a c a g a t g g t c c c c a g t c t t g t c t a c c a g t g g c g t c a a a g c c g g g g c c g g g c t c c g g t t c t t g t c t a c c a g g g g t c

2220 (in frame with gp41 peptide) IWPNPQQFLKTHQMFPGSPK IWPNPQQFLKTHQMFPGSPK a t a t g g c c c a a c c c t c a g c a g t t t c t t a a g a c c c a t c a g a t g t t t c c a g g c t c c c c c a a g t a t a c c g g g t t g g g a g t c g t c a a a g a a t t c t g g g t a g t c t a c a a a g g t c c g a g g g g g t t c

2280 DLK* DLK* g a c c t g a a a t g a c c c t g c g c c t t a t t t g a a t t a a c c a a t c a g c c t g c t t c t c g c t t c t g t c t g g a c t t t a c t g g g a c g c g g a a t a a a c t t a a t t g g t t a g t c g g a c g a a g a g c g a a g a c a

AscI 2340 *LGESPAG t c g c g c g c t t c t g c t t c c c g a g c t c t a t a a a a g a g c t c a c a a c c c c t c a c t c g g c g c g c c a g c g c g c g a a g a c g a a g g g c t c g a g a t a t t t t c t c g a g t g t t g g g g a g t g a g c c g c g c g g

EcoRI 2400 130 PKKKRK SV40 NLS TRRCFEARRYALAAWLLFPF a g t c c t c c g a c a g a a t t c a g c a c g a c g g t a a g c a a g g g c g g c c c a a a g a a g a a a c g g a a a t c a g g a g g c t g t c t t a a g t c g t g c t g c c a t t c g t t c c c g c c g g g t t t c t t c t t t g c c t t t

2460 V GGS 130 PKKKRKV (in frame with SV40 NLS) SKGEELFTG SV40 NLS SV40 NLS 1a V 5 10 SKGEELFTG EGFP 1a V 5 10 SKGEELFTG EGFP YAAGGLLLPLYALAFLQEGP g t a g g c g g c t c c c c c a a g a a g a a g c g g a a g g t a a g c a a g g g c g a a g a g c t g t t c a c c g g g c a t c c g c c g a g g g g g t t c t t c t t c g c c t t c c a t t c g t t c c c g c t t c t c g a c a a g t g g c c c

2520 (in frame with SV40 NLS) VVPILVELDGDVNGHKFSVS 15 20 25 30 VVPILVELDGDVNGHKFSVS EGFP 15 20 25 30 VVPILVELDGDVNGHKFSVS EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N HHGDQDLQVAVYVAVLEAHG g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g

2580 (in frame with SV40 NLS) GEGEGDATYGKLTLKFICTT 35 40 45 50 GEGEGDATYGKLTLKFICTT EGFP 35 40 45 50 GEGEGDATYGKLTLKFICTT EGFP ALALAIGGVALQGQLEDAGG g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g

2640 (in frame with SV40 NLS) GKLPVPWPTLVTTLTYGVQC 55 60 65 70 GKLPVPWPTLVTTLTYGVQC EGFP 55 60 65 70 GKLPVPWPTLVTTLTYGVQC EGFP ALQGHGPGGEHGGQGVAHLA g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g

2700 (in frame with SV40 NLS) FSRYPDHMKQHDFFKSAMPE 75 80 85 90 FSRYPDHMKQHDFFKSAMPE EGFP 75 80 85 90 FSRYPDHMKQHDFFKSAMPE EGFP MKQHDFFKSAMPE EAAVGVVHLLVVEELGGHGF t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t

2760 (in frame with SV40 NLS) GYVQERTIFFKDDGNYKTRA 95 100 105 110 GYVQERTIFFKDDGNYKTRA EGFP 95 100 105 110 GYVQERTIFFKDDGNYKTRA EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R GYVQERTIFFKDDGNYKTRA AVDLLAGDEELVVAVVLGAG g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g

2820 (in frame with SV40 NLS) EVKFEGDTLVNRIELKGIDF 115 120 125 130 EVKFEGDTLVNRIELKGIDF EGFP 115 120 125 130 EVKFEGDTLVNRIELKGIDF EGFP EVKFEGDTLVNRIELKGIDF LHLELAVGQHVADLQLADVE g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g

2880 (in frame with SV40 NLS) KEDGNILGHKLEYNYNSHNV 135 140 145 150 KEDGNILGHKLEYNYNSHNV EGFP 135 140 145 150 KEDGNILGHKLEYNYNSHNV EGFP KEDGNILGHKLEYNYNSHNV LLVAVDQPVLQLVVVVAVVD a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g

2940 (in frame with SV40 NLS) YIMADKQKNGIKVNFKIRHN 155 160 165 170 YIMADKQKNGIKVNFKIRHN EGFP 155 160 165 170 YIMADKQKNGIKVNFKIRHN EGFP YIMADKQKNGIKVNFKIRHN IDHGVLLLVADLHVELDAVV t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g

3000 (in frame with SV40 NLS) IEDGSVQLADHYQQNTPIGD 175 180 185 190 IEDGSVQLADHYQQNTPIGD EGFP 175 180 185 190 IEDGSVQLADHYQQNTPIGD EGFP IEDGSVQLADHYQQNTPIGD DLVAAHLEGVVVLLVGGDAV a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g

3060 (in frame with SV40 NLS) GPVLLPDNHYLSTQSALSKD 195 200 205 210 GPVLLPDNHYLSTQSALSKD EGFP 195 200 205 210 GPVLLPDNHYLSTQSALSKD EGFP GPVLLPDNHYLSTQSALSKD AGHQQGVVVVQAGLGGQAFV g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g

3120 (in frame with SV40 NLS) PNEKRDHMVLLEFVTAAGIT 215 220 225 230 PNEKRDHMVLLEFVTAAGIT EGFP 215 220 225 230 PNEKRDHMVLLEFVTAAGIT EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C PNEKRDHMVLLEFVTAAGIT GVLLAIVHDQQLEHGGGPDS c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a

NsiIBsrGI 3180 (in frame with SV40 NLS) LGMDELYK* 235 LGMDELYK * EGFP 235 LGMDELYK* EGFP LGMDELYK* EAHVLQVLLSTHM c t c g g c a t g g a c g a g c t g t a c a a g t a a t g a c g t a t g c a t g c a a t t g a c g c g t a a t t c t a c g a g c c g t a c c t g c t c g a c a t g t t c a t t a c t g c a t a c g t a c g t t a a c t g c g c a t t a a g a t g

3240 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c

AgeI 3300 PGK promoter g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c

3360 PGK promoter c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g

3420 PGK promoter a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t

BlpIBsmBIEsp3ISpeI 3480 PGK promoter c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a

3540 PGK promoter t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c

3600 PGK promoter g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g

BspEI 3660 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a

3720 1 5 MGSAIEQDG PGK promoter NeoR/KanR MGSAIEQDG t c c t c a t c t c c g g g c c t t t c g a c c t g c a g c c a a t a t g g g a t c g g c c a t t g a a c a a g a t g g a g g a g t a g a g g c c c g g a a a g c t g g a c g t c g g t t a t a c c c t a g c c g g t a a c t t g t t c t a c c

3780 10 15 20 25 LHAGSPAAWVERLFGYDWAQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LHAGSPAAWVERLFGYDWAQ a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t

3840 30 35 40 45 QTIGCSDAAVFRLSAQGRPV NeoR/KanR QTIGCSDAAVFRLSAQGRPV a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a

3900 50 55 60 65 LFVKTDLSGALNELQDEAAR NeoR/KanR LFVKTDLSGALNELQDEAAR t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c

Tth111IPflFIMscI 3960 70 75 80 85 LSWLATTGVPCAAVLDVVTE NeoR/KanR LSWLATTGVPCAAVLDVVTE g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t

4020 90 95 100 105 AGRDWLLLGEVPGQDLLSSH NeoR/KanR AGRDWLLLGEVPGQDLLSSH a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t

4080 110 115 120 125 LAPAEKVSIMADAMRRLHTL NeoR/KanR LAPAEKVSIMADAMRRLHTL c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a

4140 130 135 140 145 DPATCPFDHQAKHRIERART NeoR/KanR DPATCPFDHQAKHRIERART t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g

4200 150 155 160 165 RMEAGLVDQDDLDEEHQGLA NeoR/KanR RMEAGLVDQDDLDEEHQGLA t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g

4260 170 175 180 185 PAELFARLKARMPDGEDLVV NeoR/KanR PAELFARLKARMPDGEDLVV g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a

4320 190 195 200 205 THGDACLPNIMVENGRFSGF NeoR/KanR THGDACLPNIMVENGRFSGF g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a

RsrII 4380 210 215 220 225 IDCGRLGVADRYQDIALATR NeoR/KanR C G T T G G C T A C C C G Neo-F IDCGRLGVADRYQDIALATR c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c

4440 230 235 240 245 DIAEELGGEWADRFLVLYGI NeoR/KanR T G A T A T T Neo-F DIAEELGGEWADRFLVLYGI t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a

4500 250 255 260 265 AAPDSQRIAFYRLLDEFF* NeoR/KanR AAPDSQRIAFYRLLDEFF* c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g g g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c c

BstBISalIBamHI 4560 WPRE g g a t c c g t c g a c t t c g a a a c g c g t c t g g a a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a c c t a g g c a g c t g a a g c t t t g c g c a g a c c t t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t

4620 WPRE * A C A A C G A G G A A A A T G C G A T A C WPRE-R a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a

4680 WPRE (in frame with Factor Xa site) HRQIMSNSGTHSENEGGQIF MPLYHAIASRMAFIFSSLYK a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t

4740 WPRE (in frame with Factor Xa site) GPQQRKILLQPGNDPLTAHH SWLLSLYEELWPVVRQRGVV a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a

PflMI 4800 WPRE (in frame with Factor Xa site) ASHKSVCGGSTPANGGGTLE CTVFADATPTGWGIATTCQL g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a

4860 WPRE (in frame with Factor Xa site) KGPSESEGERNGRRFEDGGA LSGTFAFPLPIATAELIAAC c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c

4920 WPRE (in frame with Factor Xa site) KGAAPCPSPQQASVIGHHQR LARCWTGARLLGTDNSVVLS c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g

4980 WPRE (in frame with Factor Xa site) PLQRGKWPQEGTNGGPNQAP GKLTSFPWLLACVATWILRG g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c

SacII 5040 WPRE (in frame with Factor Xa site) RGEAVDR 1 RGEI Factor Xa site TSFCYVPSALNPADLPSRGL g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a

5100 WPRE LPALRPLPRLRLRPQTSRIS g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g

5160 WPRE LWAASPPGINSAVET* c c t t t g g g c c g c c t c c c c g c c t g g a a t t a a t t c t g c a g t c g a g a c c t a g a a a a a c a t g g a g g a a a c c c g g c g g a g g g g c g g a c c t t a a t t a a g a c g t c a g c t c t g g a t c t t t t t g t a c c t

5220 g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c a a c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c g t g t t

Bsu36IAcc65IKpnI 5280 g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a c t t a c c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a a t g

5340 3' LTR ( Δ U3) a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a

5400 3' LTR ( Δ U3) c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c

5460 3' LTR ( Δ U3) a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g

5520 3' LTR ( Δ U3) t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t

5580 3' LTR ( Δ U3) t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a

5640 SV40 poly(A) signal a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a

5700 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t

5760 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g

5820 SV40 ori t g g c t c t a g c t a t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g a c c g a g a t c g a t a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c

SfiI 5880 SV40 ori c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a

AvrII 5940 SV40 ori c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g

6000 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C M13/pUC Forward c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c

6060 A C T G A C C C M13/pUC Forward t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g

6120 c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a

6180 f1 ori g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g

6240 f1 ori G A G G A A A G C G A A A G A A G G G F1ori-R g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g

6300 f1 ori A F1ori-R t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a

6360 f1 ori a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c

DraIII 6420 f1 ori t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g

6480 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t

6540 f1 ori t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a

6600 f1 ori t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a

6660 AmpR promoter t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c

6720 AmpR promoter t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a

6780 1 5 10 15 20 signal sequence MSIQHFRVALIPFFAAFCLP AmpR MSIQHFRVALIPFFAAFCLP a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a

6840 signal sequence VFA 25 30 35 40 HPETLVKVKDAEDQLGA AmpR VFAHPETLVKVKDAEDQLGA g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t

6900 45 50 55 60 RVGYIELDLNSGKILESFRP AmpR RVGYIELDLNSGKILESFRP c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g

6960 65 70 75 80 EERFPMMSTFKVLLCGAVLS AmpR C G A T A C A C C G C G C C A T A A T A Amp-R EERFPMMSTFKVLLCGAVLS g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g

7020 85 90 95 100 RIDAGQEQLGRRIHYSQNDL AmpR RIDAGQEQLGRRIHYSQNDL c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c

ScaI 7080 105 110 115 120 VEYSPVTEKHLTDGMTVREL AmpR VEYSPVTEKHLTDGMTVREL g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t

7140 125 130 135 140 CSAAITMSDNTAANLLLTTI AmpR CSAAITMSDNTAANLLLTTI t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g

7200 145 150 155 160 GGPKELTAFLHNMGDHVTRL AmpR GGPKELTAFLHNMGDHVTRL g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a

7260 165 170 175 180 DRWEPELNEAIPNDERDTTM AmpR DRWEPELNEAIPNDERDTTM g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c

7320 185 190 195 200 PVAMATTLRKLLTGELLTLA AmpR PVAMATTLRKLLTGELLTLA c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a

7380 205 210 215 220 SRQQLIDWMEADKVAGPLLR AmpR SRQQLIDWMEADKVAGPLLR t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g

7440 225 230 235 240 SALPAGWFIADKSGAGERGS AmpR SALPAGWFIADKSGAGERGS t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a

7500 245 250 255 260 RGIIAALGPDGKPSRIVVIY AmpR RGIIAALGPDGKPSRIVVIY c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g

AhdI 7560 265 270 275 280 TTGSQATMDERNRQIAEIGA AmpR TTGSQATMDERNRQIAEIGA a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g

7620 285 SLIKHW* AmpR SLIKHW* t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a

7680 t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c

7740 a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g

7800 ori a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t

7860 ori c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c

7920 ori g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t

7980 ori g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t

8040 ori c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c

8100 ori t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c

8160 ori g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c

8220 ori c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c

8280 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g

8340 ori c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t

PciI 8400 a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c

8460 t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a

8520 A G C G A G T C A G T G A G C G A G L4440 g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t

8580 g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c

8640 CAP binding site c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c

8700 -35 -10 CAP binding site lac promoter c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t

8760 lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g

3ʹ 5ʹ 8804 T3 promoter G C A A T T A A C C C T C A C T A A A G G T3 g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.