Addgene pLenti CMV/TO Zeo DEST (644-1) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLenti CMV/TO Zeo DEST (644-1) Sequencing Result


Map View

EcoO109I - KflI - PpuMI (7413) AleI (7056) MfeI (6668) EcoNI (6649) NruI * (6316) PluTI (6124) SfoI (6122) NarI (6121) KasI (6120) PvuI (5828) T3 promoter T3 (5662 .. 5682) M13 rev M13 Reverse (5627 .. 5643) lac operator M13/pUC Reverse (5608 .. 5630) lac promoter CAP binding site L4440 (5395 .. 5412) PciI (5297) DrdI (5195) pBR322ori-F (5142 .. 5161) BssSI - BssSαI (5124) EBV-rev (4428 .. 4447) SV40pA-R (4374 .. 4393) BmtI (4043) NheI (4039) KpnI (3965) Acc65I (3961) SwaI (3956) PmlI (3942) DraIII (3918) FseI (3841) HpaI (47) BspDI - ClaI (169) CMV enhancer NdeI (363) SnaBI (469) CMV-F (648 .. 668) tet operator tet operator SpeI (745) lac UV5 promoter CAT-R (1078 .. 1097) BspEI (1221) AclI (1312) BsmBI - Esp3I (1449) PasI (1457) SspI (1537) ScaI (1642) BstZ17I (1760) SrfI (2096) ccdB-fwd (2197 .. 2216) BfuAI - BspMI (2340) WPRE-R (2567 .. 2587) SacII (3017) pBABE 3' (3153 .. 3173) SV40 promoter StuI (3471) AvrII (3472) MauBI (3601) SgrAI (3679) pLenti CMV/TO Zeo DEST (644-1) 7460 bp

Sequence View

HpaI 60 (in frame with gp41 peptide) RDRSIRLVNGSRRYRLTFKR cPPT/CTS RDRSIRLVNGSRRYRLTFKR a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g g t t a a c t t t t a a a a g a t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c c a a t t g a a a a t t t t c t

120 (in frame with gp41 peptide) KGGIGGYSAGERIVDIIATD cPPT/CTS KGGIGGYSAGERIVDIIATD a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g

BspDIClaI 180 (in frame with gp41 peptide) IQTKELQKQITKFKILSISL cPPT/CTS IQTKELQKQITKFKILSISL a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a t t c a a a a t t t t a t c g a t a a g c t t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t a a g t t t t a a a a t a g c t a t t c g a a c

240 (in frame with gp41 peptide) GVPRYITYGKWPAWLTAQRP CMV enhancer GVPRYITYGKWPAWLTAQRP g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g

300 (in frame with gp41 peptide) PPIDVNNDVCSHSNANRDFP CMV enhancer PPIDVNNDVCSHSNANRDFP c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t

360 (in frame with gp41 peptide) LTSMGGVFTVNCPLGSTSSV CMV enhancer LTSMGGVFTVNCPLGSTSSV t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t

NdeI 420 (in frame with gp41 peptide) SYAKYAPY* CMV enhancer SYAKYAPY* t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t

SnaBI 480 CMV enhancer t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a

540 CMV enhancer CMV promoter c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t

600 CMV promoter c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t

660 CMV promoter C G C A A A T G G G C G G CMV-F a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c

720 CMV promoter tet operator tet operator T A G G C G T G CMV-F t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c c c t a t c a g t g a t a g a g a t c t a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g g g a t a g t c a c t a t c t c t a g a

SpeI 780 tet operator attR1 c c c t a t c a g t g a t a g a g a t c g t c g a c t a g t c c a g t g t g g t g g a a t t c t g c a g a t a t c a a c g g g a t a g t c a c t a t c t c t a g c a g c t g a t c a g g t c a c a c c a c c t t a a g a c g t c t a t a g t t g

840 attR1 a a g t t t g t a c a a a a a a g c t g a a c g a g a a a c g t a a a a t g a t a t a a a t a t c a a t a t a t t a a a t t c a a a c a t g t t t t t t c g a c t t g c t c t t t g c a t t t t a c t a t a t t t a t a g t t a t a t a a t t t

900 attR1 t t a g a t t t t g c a t a a a a a a c a g a c t a c a t a a t a c t g t a a a a c a c a a c a t a t c c a g t c a c t a a t c t a a a a c g t a t t t t t t g t c t g a t g t a t t a t g a c a t t t t g t g t t g t a t a g g t c a g t g a

960 -35 -10 attR1 lac UV5 promoter a t g g c g g c c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a a t g t g t a c c g c c g g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a t t a c a c

1020 1 MEK CmR MEK t g g a t t t t g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t g g a g a a a a c c t a a a a c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a c c t c t t t

1080 5 10 15 20 KITGYTTVDISQWHRKEHFE CmR C T C CAT-R KITGYTTVDISQWHRKEHFE a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a t t t t g a g t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t a a a a c t c

1140 25 30 35 40 AFQSVAQCTYNQTVQLDITA CmR C G T A A A G T C A G T C A A C G CAT-R AFQSVAQCTYNQTVQLDITA g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t t a c g g c c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a a t g c c g g

1200 45 50 55 60 FLKTVKKNKHKFYPAFIHIL CmR FLKTVKKNKHKFYPAFIHIL t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a c a t t c t t a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t g t a a g a a

BspEI 1260 65 70 75 80 ARLMNAHPEFRMAMKDGELV CmR ARLMNAHPEFRMAMKDGELV g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a c g g t g a g c t g g t g c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t g c c a c t c g a c c a c

AclI 1320 85 90 95 100 IWDSVHPCYTVFHEQTETFS CmR IWDSVHPCYTVFHEQTETFS a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c g t t t t c a t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g c a a a a g t

1380 105 110 115 120 SLWSEYHDDFRQFLHIYSQD CmR SLWSEYHDDFRQFLHIYSQD t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c g c a a g a t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g c g t t c t a

1440 125 130 135 140 VACYGENLAYFPKGFIENMF CmR VACYGENLAYFPKGFIENMF g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a t a t g t t t c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t a t a c a a a

PasIBsmBIEsp3I 1500 145 150 155 160 FVSANPWVSFTSFDLNVANM CmR FVSANPWVSFTSFDLNVANM t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c c a a t a t g a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g g t t a t a c

SspI 1560 165 170 175 180 DNFFAPVFTMGKYYTQGDKV CmR DNFFAPVFTMGKYYTQGDKV g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a c a a g g t g c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t g t t c c a c

1620 185 190 195 200 LMPLAIQVHHAVCDGFHVGR CmR LMPLAIQVHHAVCDGFHVGR c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a g a g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t c t

ScaI 1680 205 210 215 220 MLNELQQYCDEWQGGA* CmR MLNELQQYCDEWQGGA* a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g t a a a g a t c t g g a t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g c a t t t c t a g a c c t

1740 t c c g g c t t a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c t g a t t t t t g c g g t a t a a g g c c g a a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g a c t a a a a a c g c c a t a t

BstZ17I 1800 a g a a t a t a t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g t a t g c t a t g a a g c a g c t c t t a t a t a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c a t a c g a t a c t t c g t c g

1860 g t a t t a c a g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g g c a t a t a t g a t g t c a a c a t a a t g t c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c c g t a t a t a c t a c a g t t

1920 t a t c t c c g g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c g t c t g c g t g c c g a a c g a t a g a g g c c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g c a g a c g c a c g g c t t g c

1980 c t g g a a a g c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t t t a t t g a a a t g a a c g g g a c c t t t c g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a a a t a a c t t t a c t t g c c

2040 1 5 MQFKVYTYK ccdB c t c t t t t g c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g g t t t a c a c c t a t a a a a g a g a a a a c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c c a a a t g t g g a t a t t t t

SrfI 2100 10 15 20 25 RESRYRLFVDVQSDIIDTPG ccdB g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t t g a c a c g c c c g g g c c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a a c t g t g c g g g c c c g

2160 30 35 40 45 RRMVIPLASARLLSDKVSRE ccdB g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a g t c t c c c g t g a a c c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t t c a g a g g g c a c t t g

2220 50 55 60 65 LYPVVHIGDESWRMMTTDMA ccdB G C A T G A T G A C C A C C G A T A T G ccdB-fwd t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c c a c c g a t a t g g c c a a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g g t g g c t a t a c c g g t

2280 70 75 80 85 SVPVSVIGEEVADLSHREND ccdB g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a c c g c g a a a a t g a c a c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t g g c g c t t t t a c t g t

2340 90 95 100 IKNAINLMFWGI* ccdB t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g c t c c c t t a t a c a c a a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c g a g g g a a t a t g t g t

BspMIBfuAI 2400 attR2 g c c a g t c t g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t t a t g t a g t c c g g t c a g a c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a a t a c a t c a g

2460 attR2 t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t t c t c g t a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a a g a g c a

2520 attR2 WPRE t c a g c t t t c t t g t a c a a a g t g g t t g a t a t c c a g c a c a g t g g c g g c c g c t c g a c a a t c a a c a g t c g a a a g a a c a t g t t t c a c c a a c t a t a g g t c g t g t c a c c g c c g g c g a g c t g t t a g t t g

2580 WPRE A C A A C G A G G A A A A T WPRE-R c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t

2640 WPRE (in frame with Factor Xa site) *HRQIMSNSGTHS G C G A T A C WPRE-R MPLYHAIASRMA c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a

2700 WPRE (in frame with Factor Xa site) ENEGGQIFGPQQRKILLQPG FIFSSLYKSWLLSLYEELWP t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c

2760 WPRE (in frame with Factor Xa site) NDPLTAHHASHKSVCGGSTP VVRQRGVVCTVFADATPTGW t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c

2820 WPRE (in frame with Factor Xa site) ANGGGTLEKGPSESEGERNG GIATTCQLLSGTFAFPLPIA g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t

2880 WPRE (in frame with Factor Xa site) RRFEDGGAKGAAPCPSPQQA TAELIAACLARCWTGARLLG c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t

2940 WPRE (in frame with Factor Xa site) SVIGHHQRPLQRGKWPQEGT TDNSVVLSGKLTSFPWLLAC c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c

3000 WPRE (in frame with Factor Xa site) NGGPNQAPRGEAVDR 1 RGEI Factor Xa site VATWILRGTSFCYVPSALNP t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c

SacII 3060 WPRE ADLPSRGLLPALRPLPRLRL c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g

3120 WPRE RPQTSRISLWAASPPGILQI g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g g a a t t c t g c a g a t a t c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c c t t a a g a c g t c t a t a

3180 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' SG* c c g g t t a g t a a t g a g t t t g g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g g c c a a t c a t t a c t c a a a c c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t

3240 SV40 promoter g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g

3300 SV40 promoter c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t

3360 SV40 promoter SV40 ori t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c

3420 SV40 promoter SV40 ori t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g

StuIAvrII 3480 SV40 promoter SV40 ori c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a

3540 SV40 promoter EM7 promoter t t g c a a a a a g c t c c c c c t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a a a c g t t t t t c g a g g g g g a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t

3600 1 5 10 MAKLTSAVPVLT EM7 promoter BleoR t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g

MauBI 3660 15 20 25 30 ARDVAGAVEFWTDRLGFSRD BleoR g c g c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c c g c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g

SgrAI 3720 35 40 45 50 FVEDDFAGVVRDDVTLFISA BleoR t t c g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g a a g c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c

3780 55 60 65 70 VQDQVVPDNTLAWVWVRGLD BleoR g t c c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c c a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g

3840 75 80 85 90 ELYAEWSEVVSTNFRDASGP BleoR g a g c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g c t c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c

FseI 3900 95 100 105 110 AMTEIGEQPWGREFALRDPA BleoR g c c a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c c g g t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g

SwaIPmlIDraIII 3960 115 120 125 GNCVHFVAEEQD* BleoR g g c a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t a a a t c c g t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a t t t a

Acc65IKpnI 4020 g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t

BmtINheI 4080 3' LTR ( Δ U3) a a a g g g g g g a c t g g a a g g g c t a g c t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t t t c c c c c c t g a c c t t c c c g a t c g a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c

4140 3' LTR ( Δ U3) t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t

4200 3' LTR ( Δ U3) c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a

4260 3' LTR ( Δ U3) g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g

4320 3' LTR ( Δ U3) t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a

4380 SV40 poly(A) signal C G T T A T C SV40pA-R a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c

4440 SV40 poly(A) signal G T G G T T T G T C C A A EBV-rev G T A G T G T T T A A A G SV40pA-R c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t

4500 SV40 poly(A) signal A C T C A T C EBV-rev a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g c c c c t a a c t c c g c c c a t c t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g c g g g g a t t g a g g c g g g t a g

4560 c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a

4620 a t t t a t g c a g a g g c c g a g g c c g c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t a a a t a c g t c t c c g g c t c c g g c g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c

4680 ori t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g

4740 ori t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c

4800 ori c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a

4860 ori c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g

4920 ori c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g

4980 ori g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a

5040 ori t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a

5100 ori g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g

BssSαIBssSI 5160 ori G G G A A A C G C C T G G T A T C T T pBR322ori-F g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a

DrdI 5220 ori T pBR322ori-F t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t

5280 ori g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a

PciI 5340 t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a

5400 A G C G A G L4440 a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c

5460 T C A G T G A G C G A G L4440 t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c

5520 c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g

5580 -35 CAP binding site lac promoter a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a

5640 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T M13 Reverse c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a

5700 M13 rev T3 promoter G A C M13 Reverse G C A A T T A A C C C T C A C T A A A G G T3 g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c

5760 RSV promoter c a a g c t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t g t t c g a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a

5820 RSV promoter a g c a a c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t t c g t t g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a

PvuI 5880 RSV promoter g g t a c g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c c a t g c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t

5940 RSV promoter 5' LTR (truncated) c t g a a t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t g a c t t a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a

6000 5' LTR (truncated) c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g

6060 5' LTR (truncated) t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c

6120 5' LTR (truncated) a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c

KasINarISfoIPluTI 6180 HIV-1 Ψ g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g

6240 HIV-1 Ψ t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t

6300 HIV-1 Ψ a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c

NruI* 6360 g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t

6420 t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c

6480 c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t

6540 g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t

6600 t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t

EcoNI 6660 c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t

MfeI 6720 MRDNWRSELYKYKVVKIEPL t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c

6780 GVAPTKAKRRVVQREKRAVG g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t

6840 RRE IGALFLGFLGAAGSTMGAAS t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t

6900 RRE MTLTVQARQLLSGIVQQQNN t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a

6960 RRE LLRAIEAQQHLLQLTVWGIK t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g

7020 RRE QLQARILAVERYLKDQQLLG a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t

AleI 7080 IWGCSGKLICTTAVPWNASW t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t

7140 SNKSLEQIWNHTTWMEWDRE g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t

7200 gp41 peptide INNYTSLIHSLIEESQNQQE t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t

7260 1 5 10 15 KNEQELLELDKWASL WNWFN gp41 peptide KNEQELLELDKWASLWNWFN a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t

7320 (in frame with gp41 peptide) ITNWLWYIKLFIMIVGGLVG ITNWLWYIKLFIMIVGGLVG t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a

7380 (in frame with gp41 peptide) LRIVFAVLSIVNRVRQGYSP LRIVFAVLSIVNRVRQGYSP t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a

EcoO109IPpuMIKflI 7440 (in frame with gp41 peptide) LSFQTHLPTPRGPDRPEGIE LSFQTHLPTPRGPDRPEGIE t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c

7460 (in frame with gp41 peptide) EEGGERD EEGGERD a a g a a g g t g g a g a g a g a g a c t t c t t c c a c c t c t c t c t c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.