Addgene mCherry-FKBP-PI4KB - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mCherry-FKBP-PI4KB


Map View

pBR322ori-F (7344 .. 7363) TK-pA-R (6435 .. 6454) PfoI (6356) Neo-F (6117 .. 6136) RsrII (6097) PflFI - Tth111I (5699) FspI (5683) Neo-R (5507 .. 5526) BspDI * - ClaI * (5421) CsiI - SexAI * (5170) pBABE 3' (5084 .. 5104) F1ori-R (4822 .. 4841) F1ori-F (4610 .. 4631) MluI (4467) EBV-rev (4436 .. 4455) SV40pA-R (4382 .. 4401) HpaI (4344) BclI * (4238) XbaI * (4228) ApaI (4213) PspOMI (4209) KpnI (4205) Acc65I (4201) AseI (7) NdeI (234) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) AgeI (600) mCherry-R (758 .. 776) AhdI (807) SbfI (968) mCherry-F (1026 .. 1045) BspEI (1321) BglII (1330) NotI (1343) PaeR7I - PspXI - XhoI (1367) SalI (1789) AccI (1790) KflI - PpuMI (1874) PvuI (2753) PmlI (2880) MreI (3269) mCherry-FKBP-PI4KB 7557 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

BmtINheI 600 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

AgeI 660 1 5 10 15 MVSKGEEDNMAIIKEF mCherry Kozak sequence MVSKGEEDNMAIIKEF c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g

720 20 25 30 35 MRFKVHMEGSVNGHEFEIEG mCherry MRFKVHMEGSVNGHEFEIEG a t g c g c t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c t a c g c g a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g

780 40 45 50 55 EGEGRPYEGTQTAKLKVTKG mCherry G G T T C G A C T T C C A C T G G T T mCherry-R EGEGRPYEGTQTAKLKVTKG g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a

AhdI 840 60 65 70 75 GPLPFAWDILSPQFMYGSKA mCherry GPLPFAWDILSPQFMYGSKA g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g

900 80 85 90 95 YVKHPADIPDYLKLSFPEGF mCherry YVKHPADIPDYLKLSFPEGF t a c g t g a a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g

960 100 105 110 115 KWERVMNFEDGGVVTVTQDS mCherry KWERVMNFEDGGVVTVTQDS a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g

SbfI 1020 120 125 130 135 SLQDGEFIYKVKLRGTNFPS mCherry SLQDGEFIYKVKLRGTNFPS t c c c t g c a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c a g g g a c g t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g

1080 140 145 150 155 DGPVMQKKTMGWEASSERMY mCherry C C C C G T A A T G C A G A A G A A G A mCherry-F DGPVMQKKTMGWEASSERMY g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g

1140 160 165 170 175 PEDGALKGEIKQRLKLKDGG mCherry PEDGALKGEIKQRLKLKDGG c c c g a g g a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c g g g c t c c t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g

1200 180 185 190 195 HYDAEVKTTYKAKKPVQLPG mCherry HYDAEVKTTYKAKKPVQLPG c a c t a c g a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g t g a t g c t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g

1260 200 205 210 215 AYNVNIKLDITSHNEDYTIV mCherry AYNVNIKLDITSHNEDYTIV g c c t a c a a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g c g g a t g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c

1320 220 225 230 235 EQYERAEGRHSTGGMDELYK mCherry EQYERAEGRHSTGGMDELYK g a a c a g t a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g c t t g t c a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c

PaeR7IPspXIXhoINotIBglIIBspEI 1380 (in frame with mCherry) SGLRSRSAAAGAGGAARAAL SGLRSRSAAAGAGGAARAAL t c c g g a c t c a g a t c t c g a a g c g c g g c c g c g g g a g c a g g a g g a g c a g c t c g a g c g g c g c t g a g g c c t g a g t c t a g a g c t t c g c g c c g g c g c c c t c g t c c t c c t c g t c g a g c t c g c c g c g a c

1440 1 5 10 15 20 GVQVETISPGDGRTFPKRGQ FKBP GVQVETISPGDGRTFPKRGQ g g a g t g c a g g t g g a a a c c a t c t c c c c a g g a g a c g g g c g c a c c t t c c c c a a g c g c g g c c a g c c t c a c g t c c a c c t t t g g t a g a g g g g t c c t c t g c c c g c g t g g a a g g g g t t c g c g c c g g t c

1500 25 30 35 40 TCVVHYTGMLEDGKKFDSSR FKBP TCVVHYTGMLEDGKKFDSSR a c c t g c g t g g t g c a c t a c a c c g g g a t g c t t g a a g a t g g a a a g a a a t t t g a t t c c t c c c g g t g g a c g c a c c a c g t g a t g t g g c c c t a c g a a c t t c t a c c t t t c t t t a a a c t a a g g a g g g c c

1560 45 50 55 60 DRNKPFKFMLGKQEVIRGWE FKBP DRNKPFKFMLGKQEVIRGWE g a c a g a a a c a a g c c c t t t a a g t t t a t g c t a g g c a a g c a g g a g g t g a t c c g a g g c t g g g a a c t g t c t t t g t t c g g g a a a t t c a a a t a c g a t c c g t t c g t c c t c c a c t a g g c t c c g a c c c t t

1620 65 70 75 80 EGVAQMSVGQRAKLTISPDY FKBP EGVAQMSVGQRAKLTISPDY g a a g g g g t t g c c c a g a t g a g t g t g g g t c a g a g a g c c a a a c t g a c t a t a t c t c c a g a t t a t c t t c c c c a a c g g g t c t a c t c a c a c c c a g t c t c t c g g t t t g a c t g a t a t a g a g g t c t a a t a

1680 85 90 95 100 AYGATGHPGIIPPHATLVFD FKBP AYGATGHPGIIPPHATLVFD g c c t a t g g t g c c a c t g g g c a c c c a g g c a t c a t c c c a c c a c a t g c c a c t c t c g t c t t c g a t c g g a t a c c a c g g t g a c c c g t g g g t c c g t a g t a g g g t g g t g t a c g g t g a g a g c a g a a g c t a

1740 105 VELLKLE (in frame with FKBP) SAGGSAGGSAGGS FKBP VELLKLESAGGSAGGSAGGS g t g g a g c t t c t a a a a c t g g a a a g t g c t g g t g g t a g t g c t g g t g g t a g t g c t g g t g g t a g t c a c c t c g a a g a t t t t g a c c t t t c a c g a c c a c c a t c a c g a c c a c c a t c a c g a c c a c c a t c a

SalIAccI 1800 (in frame with FKBP) AGGSAGGPRAQASNSAVDMG AGGSAGGPRAQASNSAVDMG *AEFEATSMP g c t g g t g g t a g t g c t g g t g g t c c g c g a g c t c a a g c t t c g a a t t c t g c a g t c g a c a t g g g a c g a c c a c c a t c a c g a c c a c c a g g c g c t c g a g t t c g a a g c t t a a g a c g t c a g c t g t a c c c t

1860 (in frame with FKBP) DTVVEPAPLKPTSEPTSGPP DTVVEPAPLKPTSEPTSGPP SVTTSGAGKFGVESGVEPGG g a t a c a g t a g t g g a g c c t g c c c c c t t g a a g c c a a c t t c t g a g c c c a c t t c t g g c c c a c c a c t a t g t c a t c a c c t c g g a c g g g g g a a c t t c g g t t g a a g a c t c g g g t g a a g a c c g g g t g g t

PpuMIKflI 1920 (in frame with FKBP) GNNGGSLLSVITEGVGELSV GNNGGSLLSVITEGVGELSV PFLPPDRSLTMVSPTPSSDT g g g a a t a a t g g g g g g t c c c t g c t a a g t g t c a t c a c g g a g g g g g t c g g g g a a c t a t c a g t g c c c t t a t t a c c c c c c a g g g a c g a t t c a c a g t a g t g c c t c c c c c a g c c c c t t g a t a g t c a c

1980 (in frame with FKBP) IDPEVAQKACQEVLEKVKLL IDPEVAQKACQEVLEKVKLL ISGSTAWFAQWSTNSFTLSK a t t g a c c c t g a g g t g g c c c a g a a g g c c t g c c a g g a g g t g t t g g a g a a a g t c a a g c t t t t g t a a c t g g g a c t c c a c c g g g t c t t c c g g a c g g t c c t c c a c a a c c t c t t t c a g t t c g a a a a c

2040 (in frame with FKBP) HGGVAVSSRGTPLELVNGDG HGGVAVSSRGTPLELVNGDG CPPTATELLPVGSSNTLPSP c a t g g a g g c g t g g c a g t c t c t a g c a g a g g c a c c c c a c t g g a g t t g g t c a a t g g g g a t g g t g t a c c t c c g c a c c g t c a g a g a t c g t c t c c g t g g g g t g a c c t c a a c c a g t t a c c c c t a c c a

2100 (in frame with FKBP) VDSEIRCLDDPPAQIREEED VDSEIRCLDDPPAQIREEED TSLSIRQRSSGGAWILSSSS g t g g a c a g t g a g a t c c g t t g c c t a g a t g a t c c a c c t g c c c a g a t c a g g g a g g a g g a a g a t c a c c t g t c a c t c t a g g c a a c g g a t c t a c t a g g t g g a c g g g t c t a g t c c c t c c t c c t t c t a

2160 (in frame with FKBP) EMGAAVASGTAKGARRRRQN EMGAAVASGTAKGARRRRQN SIPAATAEPVALPALLRRCF g a g a t g g g g g c c g c t g t g g c c t c a g g c a c a g c c a a a g g a g c a a g a a g a c g g c g g c a g a a c c t c t a c c c c c g g c g a c a c c g g a g t c c g t g t c g g t t t c c t c g t t c t t c t g c c g c c g t c t t g

2220 (in frame with FKBP) NSAKQSWLLRLFESKLFDIS NSAKQSWLLRLFESKLFDIS LEALCDQSSLSNSDFSNSME a a c t c a g c t a a a c a g t c t t g g c t g c t g a g g c t g t t t g a g t c a a a a c t g t t t g a c a t c t c c t t g a g t c g a t t t g t c a g a a c c g a c g a c t c c g a c a a a c t c a g t t t t g a c a a a c t g t a g a g g

2280 (in frame with FKBP) MAISYLYNSKEPGVQAYIGN MAISYLYNSKEPGVQAYIGN MAM a t g g c c a t t t c a t a c c t g t a t a a c t c c a a g g a g c c t g g a g t a c a a g c c t a c a t t g g c a a c t a c c g g t a a a g t a t g g a c a t a t t g a g g t t c c t c g g a c c t c a t g t t c g g a t g t a a c c g t t g

2340 (in frame with FKBP) RLFCFRNEDVDFYLPQLLNM RLFCFRNEDVDFYLPQLLNM c g g c t c t t c t g c t t t c g c a a c g a g g a c g t g g a c t t c t a t c t g c c c c a g t t g c t t a a c a t g g c c g a g a a g a c g a a a g c g t t g c t c c t g c a c c t g a a g a t a g a c g g g g t c a a c g a a t t g t a c

2400 (in frame with FKBP) YIHMDEDVGDAIKPYIVHRC YIHMDEDVGDAIKPYIVHRC t a c a t c c a c a t g g a t g a g g a c g t g g g t g a t g c c a t t a a g c c c t a c a t a g t c c a c c g t t g c a t g t a g g t g t a c c t a c t c c t g c a c c c a c t a c g g t a a t t c g g g a t g t a t c a g g t g g c a a c g

2460 (in frame with FKBP) RQSINFSLQCALLLGAYSSD RQSINFSLQCALLLGAYSSD c g c c a g a g c a t t a a c t t t t c c c t c c a g t g t g c c c t g t t g c t t g g g g c c t a t t c t t c a g a c g c g g t c t c g t a a t t g a a a a g g g a g g t c a c a c g g g a c a a c g a a c c c c g g a t a a g a a g t c t g

2520 (in frame with FKBP) MHISTQRHSRGTKLRKLILS MHISTQRHSRGTKLRKLILS a t g c a c a t t t c c a c t c a a c g a c a c t c c c g t g g g a c c a a g c t a c g g a a g c t g a t c c t c t c a t a c g t g t a a a g g t g a g t t g c t g t g a g g g c a c c c t g g t t c g a t g c c t t c g a c t a g g a g a g t

2580 (in frame with FKBP) DELKPAHRKRELPSLSPAPD DELKPAHRKRELPSLSPAPD g a t g a g c t a a a g c c a g c t c a c a g g a a g a g g g a g c t g c c c t c c t t g a g c c c g g c c c c t g a c c t a c t c g a t t t c g g t c g a g t g t c c t t c t c c c t c g a c g g g a g g a a c t c g g g c c g g g g a c t g

2640 (in frame with FKBP) TGLSPSKRTHQRSKSDATAS TGLSPSKRTHQRSKSDATAS a c a g g g c t g t c t c c c t c c a a a a g g a c t c a c c a g c g c t c t a a g t c a g a t g c c a c t g c c a g c t g t c c c g a c a g a g g g a g g t t t t c c t g a g t g g t c g c g a g a t t c a g t c t a c g g t g a c g g t c g

2700 (in frame with FKBP) ISLSSNLKRTASNPKVENED ISLSSNLKRTASNPKVENED a t a a g t c t c a g c a g c a a c c t g a a a c g a a c a g c c a g c a a c c c t a a a g t g g a g a a t g a g g a t t a t t c a g a g t c g t c g t t g g a c t t t g c t t g t c g g t c g t t g g g a t t t c a c c t c t t a c t c c t a

PvuI 2760 (in frame with FKBP) EPVRLAPEREFIKSLMAIGK EPVRLAPEREFIKSLMAIGK g a g c c t g t t c g a c t g g c t c c t g a g a g a g a a t t c a t c a a g t c c c t g a t g g c g a t c g g c a a g c t c g g a c a a g c t g a c c g a g g a c t c t c t c t t a a g t a g t t c a g g g a c t a c c g c t a g c c g t t c

2820 (in frame with FKBP) RLATLPTKEQKTQRLISELS RLATLPTKEQKTQRLISELS *LEG c g g c t g g c c a c g c t c c c c a c c a a a g a g c a g a a a a c a c a g a g g c t g a t c t c a g a g c t c t c c g c c g a c c g g t g c g a g g g g t g g t t t c t c g t c t t t t g t g t c t c c g a c t a g a g t c t c g a g a g g

PmlI 2880 (in frame with FKBP) LLNHKLPARVWLPTAGFDHH LLNHKLPARVWLPTAGFDHH QEVMLERGSDPQGSSAKVVV c t g c t c a a c c a t a a g c t c c c t g c c c g a g t c t g g c t g c c c a c t g c t g g c t t t g a c c a c c a c g a c g a g t t g g t a t t c g a g g g a c g g g c t c a g a c c g a c g g g t g a c g a c c g a a a c t g g t g g t g

2940 (in frame with FKBP) VVRVPHTQAVVLNSKDKAPY VVRVPHTQAVVLNSKDKAPY HDTYGVCLSNDEVGLVLSGV g t g g t c c g t g t a c c c c a c a c a c a g g c t g t t g t c c t c a a c t c c a a g g a c a a g g c t c c c t a c c a c c a g g c a c a t g g g g t g t g t g t c c g a c a a c a g g a g t t g a g g t t c c t g t t c c g a g g g a t g

3000 (in frame with FKBP) LIYVEVLECENFDTTSVPAR LIYVEVLECENFDTTSVPAR QNIHFDKFTFVKVGGTDRGP c t g a t t t a t g t g g a a g t c c t t g a a t g t g a a a a c t t t g a c a c c a c c a g t g t c c c t g c c c g g g a c t a a a t a c a c c t t c a g g a a c t t a c a c t t t t g a a a c t g t g g t g g t c a c a g g g a c g g g c c

3060 (in frame with FKBP) IPENRIRSTRSVENLPECGI IPENRIRSTRSVENLPECGI DGLVSNPTRPGYFVQGFTTN a t c c c c g a g a a c c g a a t t c g g a g t a c g a g g t c c g t a g a a a a c t t g c c c g a a t g t g g t a t t t a g g g g c t c t t g g c t t a a g c c t c a t g c t c c a g g c a t c t t t t g a a c g g g c t t a c a c c a t a a

3120 (in frame with FKBP) THEQRAGSFSTVPNYDNDDE THEQRAGSFSTVPNYDNDDE GMLLSSAAEASHGVIVVIIL a c c c a t g a g c a g c g a g c t g g c a g c t t c a g c a c t g t g c c c a a c t a t g a c a a c g a t g a t g a g t g g g t a c t c g t c g c t c g a c c g t c g a a g t c g t g a c a c g g g t t g a t a c t g t t g c t a c t a c t c

3180 (in frame with FKBP) AWSVDDIGELQVELPEVHTN AWSVDDIGELQVELPEVHTN GPRHIVYALQLHLEGFHMGV g c c t g g t c g g t g g a t g a c a t a g g c g a g c t g c a a g t g g a g c t c c c c g a a g t g c a t a c c a a c c g g a c c a g c c a c c t a c t g t a t c c g c t c g a c g t t c a c c t c g a g g g g c t t c a c g t a t g g t t g

3240 (in frame with FKBP) SCDNISQFSVDSITSQESKE SCDNISQFSVDSITSQESKE ATVVDGLERHVADGALLALL a g c t g t g a c a a c a t c t c c c a g t t c t c t g t g g a c a g c a t c a c c a g c c a g g a g a g c a a g g a g t c g a c a c t g t t g t a g a g g g t c a a g a g a c a c c t g t c g t a g t g g t c g g t c c t c t c g t t c c t c

MreI 3300 (in frame with FKBP) PVFIAAGDIRRRLSEQLAHT PVFIAAGDIRRRLSEQLAHT RHENCCPVDAPAKRFLQSMG c c t g t g t t c a t t g c a g c a g g g g a c a t c c g c c g g c g c c t t t c g g a a c a g c t g g c t c a t a c c g g a c a c a a g t a a c g t c g t c c c c t g t a g g c g g c c g c g g a a a g c c t t g t c g a c c g a g t a t g g

3360 (in frame with FKBP) PTAFKRDPEDPSAVALKEPW PTAFKRDPEDPSAVALKEPW RCGEFSVWFIRRCNSEFLGP c c g a c a g c c t t c a a a c g a g a c c c a g a a g a t c c t t c t g c a g t t g c t c t c a a a g a g c c c t g g g g c t g t c g g a a g t t t g c t c t g g g t c t t c t a g g a a g a c g t c a a c g a g a g t t t c t c g g g a c c

3420 (in frame with FKBP) QEKVRRIREGSPYGHLPNWR QEKVRRIREGSPYGHLPNWR LLFYPPDSLAGGVAM c a g g a g a a a g t a c g g c g g a t c a g a g a g g g c t c c c c c t a c g g c c a t c t c c c c a a t t g g c g g g t c c t c t t t c a t g c c g c c t a g t c t c t c c c g a g g g g g a t g c c g g t a g a g g g g t t a a c c g c c

3480 (in frame with FKBP) LLSVIVKCGDDLRQELLAFQ LLSVIVKCGDDLRQELLAFQ c t c c t g t c a g t c a t t g t c a a g t g t g g g g a t g a c c t t c g g c a a g a g c t t c t g g c c t t t c a g g a g g a c a g t c a g t a a c a g t t c a c a c c c c t a c t g g a a g c c g t t c t c g a a g a c c g g a a a g t c

3540 (in frame with FKBP) VLKQLQSIWEQERVPLWIKP VLKQLQSIWEQERVPLWIKP g t g t t g a a g c a a c t g c a g t c c a t t t g g g a a c a g g a g c g a g t g c c c c t t t g g a t c a a g c c a c a c a a c t t c g t t g a c g t c a g g t a a a c c c t t g t c c t c g c t c a c g g g g a a a c c t a g t t c g g t

3600 (in frame with FKBP) YKILVISADSGMIEPVVNAV YKILVISADSGMIEPVVNAV t a c a a g a t t c t t g t g a t t t c g g c t g a t a g t g g c a t g a t t g a a c c a g t g g t c a a t g c t g t g a t g t t c t a a g a a c a c t a a a g c c g a c t a t c a c c g t a c t a a c t t g g t c a c c a g t t a c g a c a c

3660 (in frame with FKBP) SIHQVKKQSQLSLLDYFLQE SIHQVKKQSQLSLLDYFLQE t c c a t c c a t c a g g t g a a g a a a c a g t c a c a g c t c t c c t t g c t c g a t t a c t t c c t a c a g g a g a g g t a g g t a g t c c a c t t c t t t g t c a g t g t c g a g a g g a a c g a g c t a a t g a a g g a t g t c c t c

3720 (in frame with FKBP) HGSYTTEAFLSAQRNFVQSC HGSYTTEAFLSAQRNFVQSC c a c g g c a g t t a c a c c a c t g a g g c a t t c c t c a g t g c a c a g c g c a a t t t t g t g c a a a g t t g t g t g c c g t c a a t g t g g t g a c t c c g t a a g g a g t c a c g t g t c g c g t t a a a a c a c g t t t c a a c a

3780 (in frame with FKBP) AGYCLVCYLLQVKDRHNGNI AGYCLVCYLLQVKDRHNGNI g c t g g g t a c t g c t t g g t c t g c t a c c t g c t g c a a g t c a a g g a c a g a c a c a a t g g g a a t a t c c g a c c c a t g a c g a a c c a g a c g a t g g a c g a c g t t c a g t t c c t g t c t g t g t t a c c c t t a t a g

3840 (in frame with FKBP) LLDAEGHIIHIDFGFILSSS LLDAEGHIIHIDFGFILSSS c t t t t g g a c g c a g a a g g c c a c a t c a t c c a c a t c g a c t t t g g c t t c a t c c t c t c c a g c t c a g a a a a c c t g c g t c t t c c g g t g t a g t a g g t g t a g c t g a a a c c g a a g t a g g a g a g g t c g a g t

3900 (in frame with FKBP) PRNLGFETSAFKLTTEFVDV PRNLGFETSAFKLTTEFVDV c c c c g a a a t c t g g g c t t t g a g a c g t c a g c c t t t a a g c t g a c c a c a g a g t t t g t g g a t g t g g g g g c t t t a g a c c c g a a a c t c t g c a g t c g g a a a t t c g a c t g g t g t c t c a a a c a c c t a c a c

3960 (in frame with FKBP) MGGLDGDMFNYYKMLMLQGL MGGLDGDMFNYYKMLMLQGL a t g g g c g g c c t g g a t g g c g a c a t g t t c a a c t a c t a t a a g a t g c t g a t g c t g c a a g g g c t g t a c c c g c c g g a c c t a c c g c t g t a c a a g t t g a t g a t a t t c t a c g a c t a c g a c g t t c c c g a c

4020 (in frame with FKBP) IAARKHMDKVVQIVEIMQQG IAARKHMDKVVQIVEIMQQG a t t g c c g c t c g g a a a c a c a t g g a c a a g g t g g t g c a g a t c g t g g a g a t c a t g c a g c a a g g t t a a c g g c g a g c c t t t g t g t a c c t g t t c c a c c a c g t c t a g c a c c t c t a g t a c g t c g t t c c a

4080 (in frame with FKBP) SQLPCFHGSSTIRNLKERFH SQLPCFHGSSTIRNLKERFH t c t c a g c t t c c t t g c t t c c a t g g c t c c a g c a c c a t t c g a a a c c t c a a a g a g a g g t t c c a c a g a g t c g a a g g a a c g a a g g t a c c g a g g t c g t g g t a a g c t t t g g a g t t t c t c t c c a a g g t g

4140 (in frame with FKBP) MSMTEEQLQLLVEQMVDGSM MSMTEEQLQLLVEQMVDGSM a t g a g c a t g a c t g a g g a g c a g c t g c a g c t g c t g g t g g a g c a g a t g g t g g a t g g c a g t a t g t a c t c g t a c t g a c t c c t c g t c g a c g t c g a c g a c c a c c t c g t c t a c c a c c t a c c g t c a t a c

4200 (in frame with FKBP) RSITTKLYDGFQYLTNGIM* RSITTKLYDGFQYLTNGIM* c g g t c t a t c a c c a c c a a a c t c t a t g a c g g c t t c c a g t a c c t c a c c a a c g g c a t c a t g t g a g c c a g a t a g t g g t g g t t t g a g a t a c t g c c g a a g g t c a t g g a g t g g t t g c c g t a g t a c a c t

BclI*XbaI*Acc65IKpnIPspOMIApaI 4260 g g t a c c g c g g g c c c g g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a c c a t g g c g c c c g g g c c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t

4320 t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a

HpaI 4380 SV40 poly(A) signal a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t

4440 SV40 poly(A) signal G T G G T EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a

MluI 4500 SV40 poly(A) signal f1 ori T T G T C C A A A C T C A T C EBV-rev t t g t c c a a a c t c a t c a a t g t a t c t t a a c g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a a c a g g t t t g a g t a g t t a c a t a g a a t t g c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t

4560 f1 ori a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t

4620 f1 ori G G T C A A A C C T T F1ori-F a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t

4680 f1 ori G T T C T C A G G T G F1ori-F c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t

4740 f1 ori g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c

4800 f1 ori t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g

4860 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g

4920 f1 ori a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t

4980 f1 ori AmpR promoter g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a

5040 AmpR promoter c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t

5100 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T pBABE 3' a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t

5160 SV40 promoter SV40 enhancer C C C A pBABE 3' g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a

SexAI*CsiI 5220 SV40 promoter SV40 enhancer t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g

5280 SV40 promoter SV40 enhancer SV40 ori a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t

5340 SV40 promoter SV40 ori a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t

5400 SV40 promoter SV40 ori g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t

BspDI*ClaI* 5460 1 MIE SV40 promoter NeoR/KanR 1 MIE SV40 ori NeoR/KanR MIE g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t

5520 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR G A T A A G C C G A T A C T Neo-R QDGLHAGSPAAWVERLFGYD a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t

5580 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR G A C C C G Neo-R WAQQTIGCSDAAVFRLSAQG c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c

5640 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR RPVLFVKTDLSGALNELQDE g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t

Tth111IPflFIFspI 5700 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR AARLSWLATTGVPCAAVLDV g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a

5760 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR VTEAGRDWLLLGEVPGQDLL t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a

5820 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR SSHLAPAEKVSIMADAMRRL *RVKSRGLFYGDHSICHPPQ g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a

5880 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR HTLDPATCPFDHQAKHRIER MRKIRSGAWEVVLRFMADLS g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c

5940 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR ARTRMEAGLVDQDDLDEEHQ CTSPHFGTKDILIIQVFLML a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t

6000 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR GLAPAELFARLKASMPDGED PERWGFQEGPELRAHGVALI g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t

6060 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR LVVTHGDACLPNIMVENGRF EDHGMAIGAQRIDHHFIAAK t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a

RsrII 6120 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR C G T T Neo-F SGFIDCGRLGVADRYQDIAL RSEDVTAPQTHRVAILVYRQ t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a

6180 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR G G C T A C C C G T G A T A T T Neo-F ATRDIAEELGGEWADRFLVL SGTINSFLKAAFPSVAEEHK g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a

6240 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR YGIAAPDSQRIAFYRLLDEF VTDGSGIRLADGEIAKKVLE t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a

6300 265 F* NeoR/KanR 265 F* NeoR/KanR MTDQATPNLPS F* ESRSEPTRFSRGLSAWGAM c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t

PfoI 6360 RDFDSTAAFYERLGFGIVFR c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c

6420 DAGWMILQRGDLMLEFFAHP g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a

6480 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R RGRLTETRKETIPEGTRAMT a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c

6540 HSV TK poly(A) signal AIKRQNKTHGVGSFVHKRGV g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a

6600 RSQGWHSVDTPPRPHWGQYA c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g

6660 RVSSFSPPHPPSSGEGPGLA c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t

6720 ANVGAAGPAIASGYSYIL* g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a

6780 g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g

6840 a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c

6900 ori a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t

6960 ori a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c

7020 ori a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c

7080 ori t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c

7140 ori t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t

7200 ori t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g

7260 ori t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g

7320 ori a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t

7380 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a

7440 ori c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c

7500 ori a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g

3ʹ 5ʹ 7557 a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.