Addgene pEGFP-sfCherry11-ß-actin Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pEGFP-sfCherry11-ß-actin Sequencing Result


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PciI (5133) pBR322ori-F (4978 .. 4997) ApaLI (4819) TK-pA-R (4069 .. 4088) PfoI (3990) BstBI (3897) Neo-F (3751 .. 3770) RsrII (3731) FspI (3317) Neo-R (3141 .. 3160) BspDI * - ClaI * (3055) StuI (3036) SfiI (2990) CsiI - SexAI * (2804) pBABE 3' (2718 .. 2738) AseI (14) NdeI (241) SnaBI (347) CMV-F (526 .. 546) NheI (598) BmtI (602) AfeI (603) AgeI (607) BsrGI (621) AleI (629) BglII (680) EcoRV (733) SalI (749) AccI (750) TspMI - XmaI (801) BbsI (802) SmaI (803) BstEII (1195) PpuMI (1270) BmgBI (1579) Bpu10I (1719) ScaI (1730) BamHI (1850) XbaI * (1862) BclI * (1872) MfeI (1965) HpaI (1978) SV40pA-R (2016 .. 2035) EBV-rev (2070 .. 2089) MluI (2101) F1ori-F (2244 .. 2265) F1ori-R (2456 .. 2475) pEGFP-sfCherry11-ß-actin 5184 bp

Sequence View

AseI 60 c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c

120 CMV enhancer a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g

180 CMV enhancer g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a

240 CMV enhancer g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g

NdeI 300 CMV enhancer a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c

SnaBI 360 CMV enhancer c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c

420 CMV enhancer CMV promoter c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a

480 CMV promoter c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t

540 CMV promoter C G C A A A T G G G C G G T A CMV-F a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t

NheI 600 CMV promoter G G C G T G CMV-F g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a

AleIBsrGIAgeIBmtIAfeI 660 MYTIVEQYERAEGR a g c g c t a c c g g t c g c c a c c a t g t a c a c c a t c g t g g a g c a g t a c g a g a g a g c c g a g g g c a g t c g c g a t g g c c a g c g g t g g t a c a t g t g g t a g c a c c t c g t c a t g c t c t c t c g g c t c c c g t c

BglII 720 HSTVRLRSGSGGGSASGGSG a c a c a g c a c c g t c c g g c t c a g a t c t g g c a g c g g t g g a g g c a g c g c a t c c g g c g g a a g c g g t g t g t c g t g g c a g g c c g a g t c t a g a c c g t c g c c a c c t c c g t c g c g t a g g c c g c c t t c g c c

SalIAccIEcoRV 780 SDDDIAALVVDNGSGMCKAG a a g c g a t g a t g a t a t c g c c g c g c t c g t c g t c g a c a a c g g c t c c g g c a t g t g c a a g g c c g g t t c g c t a c t a c t a t a g c g g c g c g a g c a g c a g c t g t t g c c g a g g c c g t a c a c g t t c c g g c c

TspMIXmaIBbsISmaI 840 FAGDDAPRAVFPSIVGRPRH c t t c g c g g g c g a c g a t g c c c c c c g g g c c g t c t t c c c c t c c a t c g t g g g g c g c c c c a g g c a g a a g c g c c c g c t g c t a c g g g g g g c c c g g c a g a a g g g g a g g t a g c a c c c c g c g g g g t c c g t

900 QGVMVGMGQKDSYVGDEAQS c c a g g g c g t g a t g g t g g g c a t g g g t c a g a a g g a t t c c t a t g t g g g c g a c g a g g c c c a g a g g g t c c c g c a c t a c c a c c c g t a c c c a g t c t t c c t a a g g a t a c a c c c g c t g c t c c g g g t c t c

960 KRGILTLKYPIEHGIVTNWD c a a g a g a g g c a t c c t c a c c c t g a a g t a c c c c a t c g a g c a c g g c a t c g t c a c c a a c t g g g a g t t c t c t c c g t a g g a g t g g g a c t t c a t g g g g t a g c t c g t g c c g t a g c a g t g g t t g a c c c t

1020 DMEKIWHHTFYNELRVAPEE c g a c a t g g a g a a a a t c t g g c a c c a c a c c t t c t a c a a t g a g c t g c g t g t g g c t c c c g a g g a g c t g t a c c t c t t t t a g a c c g t g g t g t g g a a g a t g t t a c t c g a c g c a c a c c g a g g g c t c c t

1080 HPVLLTEAPLNPKANREKMT g c a c c c c g t g c t g c t g a c c g a g g c c c c c c t g a a c c c c a a g g c c a a c c g c g a g a a g a t g a c c g t g g g g c a c g a c g a c t g g c t c c g g g g g g a c t t g g g g t t c c g g t t g g c g c t c t t c t a c t g

1140 QIMFETFNTPAMYVAIQAVL * c c a g a t c a t g t t t g a g a c c t t c a a c a c c c c a g c c a t g t a c g t t g c t a t c c a g g c t g t g c t g g t c t a g t a c a a a c t c t g g a a g t t g t g g g g t c g g t a c a t g c a a c g a t a g g t c c g a c a c g a

BstEII 1200 SLYASGRTTGIVMDSGDGVT GQVGRATGSADHHVGTVPDG a t c c c t g t a c g c c t c t g g c c g t a c c a c t g g c a t c g t g a t g g a c t c c g g t g a c g g g g t c a c t a g g g a c a t g c g g a g a c c g g c a t g g t g a c c g t a g c a c t a c c t g a g g c c a c t g c c c c a g t g

1260 HTVPIYEGYALPHAILRLDL VSHGDVLPIGEGMGDQTQVQ c c a c a c t g t g c c c a t c t a c g a g g g g t a t g c c c t c c c c c a t g c c a t c c t g c g t c t g g a c c t g g t g t g a c a c g g g t a g a t g c t c c c c a t a c g g g a g g g g g t a c g g t a g g a c g c a g a c c t g g a

PpuMI 1320 AGRDLTDYLMKILTERGYSF SAPVQSVVEHLDEGLAAVAE g g c t g g c c g g g a c c t g a c t g a c t a c c t c a t g a a g a t c c t c a c c g a g c g c g g c t a c a g c t t c c g a c c g g c c c t g g a c t g a c t g a t g g a g t a c t t c t a g g a g t g g c t c g c g c c g a t g t c g a a

1380 TTTAEREIVRDIKEKLCYVA GGRGLPFDHTVNLLLQAVDG c a c c a c c a c g g c c g a g c g g g a a a t c g t g c g t g a c a t t a a g g a g a a g c t g t g c t a c g t c g c g t g g t g g t g c c g g c t c g c c c t t t a g c a c g c a c t g t a a t t c c t c t t c g a c a c g a t g c a g c g

1440 LDFEQEMATAASSSSLEKSY QVELLLHGRSSGAGGQLLAV c c t g g a c t t c g a g c a a g a g a t g g c c a c g g c t g c t t c c a g c t c c t c c c t g g a g a a g a g c t a g g a c c t g a a g c t c g t t c t c t a c c g g t g c c g a c g a a g g t c g a g g a g g g a c c t c t t c t c g a t

1500 ELPDGQVITIGNERFRCPEA LQRVALDDGNAILPEAARLC c g a g c t g c c t g a c g g c c a g g t c a t c a c c a t t g g c a a t g a g c g g t t c c g c t g c c c t g a g g c g c t c g a c g g a c t g c c g g t c c a g t a g t g g t a a c c g t t a c t c g c c a a g g c g a c g g g a c t c c g

1560 LFQPSFLGMESCGIHETTFN EELRGEQAHLGTADVFSGEV a c t c t t c c a g c c t t c c t t c c t g g g c a t g g a g t c c t g t g g c a t c c a c g a a a c t a c c t t c a a t g a g a a g g t c g g a a g g a a g g a c c c g t a c c t c a g g a c a c c g t a g g t g c t t t g a t g g a a g t t

BmgBI 1620 SIMKCDVDIRKDLYANTVLS GDHLTVHVDAFVQVGVCHQR c t c c a t c a t g a a g t g t g a c g t g g a c a t c c g c a a a g a c c t g t a c g c c a a c a c a g t g c t g t c g a g g t a g t a c t t c a c a c t g c a c c t g t a g g c g t t t c t g g a c a t g c g g t t g t g t c a c g a c a g

1680 GGTTMYPGIADRMQKEITAL AAGGHVRANGVPHLLLDSGQ t g g c g g c a c c a c c a t g t a c c c t g g c a t t g c c g a c a g g a t g c a g a a g g a g a t c a c t g c c c t a c c g c c g t g g t g g t a c a t g g g a c c g t a a c g g c t g t c c t a c g t c t t c c t c t a g t g a c g g g a

ScaIBpu10I 1740 APSTMKIKIIAPPERKYSVW CGACHLDLDNSRRLALVGHP g g c a c c c a g c a c a a t g a a g a t c a a g a t c a t t g c t c c t c c t g a g c g c a a g t a c t c c g t g t g c c g t g g g t c g t g t t a c t t c t a g t t c t a g t a a c g a g g a g g a c t c g c g t t c a t g a g g c a c a c

1800 IGGSILASLSTFQQMWISKQ DAAGDQGRQGGELLHPDALL g a t c g g c g g c t c c a t c c t g g c c t c g c t g t c c a c c t t c c a g c a g a t g t g g a t c a g c a a g c a c t a g c c g c c g a g g t a g g a c c g g a g c g a c a g g t g g a a g g t c g t c t a c a c c t a g t c g t t c g t

BamHI 1860 EYDESGPSIVHRKCF* LIVLGAGGDDVAFAELSGGS g g a g t a t g a c g a g t c c g g c c c c t c c a t c g t c c a c c g c a a a t g c t t c t a g g g a t c c a c c g g c c t c a t a c t g c t c a g g c c g g g g a g g t a g c a g g t g g c g t t t a c g a a g a t c c c t a g g t g g c c

BclI*XbaI* 1920 RSLQDYDAM a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t

HpaIMfeI 1980 SV40 poly(A) signal a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t

2040 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t

2100 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t

MluI 2160 f1 ori a c g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a t g c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t

2220 f1 ori g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t

2280 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c

2340 f1 ori a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a

2400 f1 ori c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c

2460 f1 ori A G G G A F1ori-R g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t

2520 f1 ori A G A A A G C G A A A G G A G F1ori-R a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t

2580 f1 ori c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c

2640 AmpR promoter g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g

2700 AmpR promoter SV40 promoter SV40 enhancer t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c

2760 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g

SexAI*CsiI 2820 SV40 promoter SV40 enhancer a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g

2880 SV40 promoter SV40 enhancer c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a

2940 SV40 promoter SV40 enhancer SV40 ori a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g

SfiI 3000 SV40 promoter SV40 ori g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t

BspDI*ClaI*StuI 3060 SV40 promoter SV40 ori g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g

3120 1 5 10 MIEQDGLHAGS NeoR/KanR 1 5 10 MIEQDGLHAGS NeoR/KanR MIEQDGLHAGS a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g

3180 15 20 25 30 PAAWVERLFGYDWAQQTIGC NeoR/KanR 15 20 25 30 PAAWVERLFGYDWAQQTIGC NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R PAAWVERLFGYDWAQQTIGC c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a

3240 35 40 45 50 SDAAVFRLSAQGRPVLFVKT NeoR/KanR 35 40 45 50 SDAAVFRLSAQGRPVLFVKT NeoR/KanR SDAAVFRLSAQGRPVLFVKT c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c

3300 55 60 65 70 DLSGALNELQDEAARLSWLA NeoR/KanR 55 60 65 70 DLSGALNELQDEAARLSWLA NeoR/KanR DLSGALNELQDEAARLSWLA a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t

FspI 3360 75 80 85 90 TTGVPCAAVLDVVTEAGRDW NeoR/KanR 75 80 85 90 TTGVPCAAVLDVVTEAGRDW NeoR/KanR TTGVPCAAVLDVVTEAGRDW c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g

3420 95 100 105 110 LLLGEVPGQDLLSSHLAPAE NeoR/KanR 95 100 105 110 LLLGEVPGQDLLSSHLAPAE NeoR/KanR LLLGEVPGQDLLSSHLAPAE *RVKSRGL t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t

3480 115 120 125 130 KVSIMADAMRRLHTLDPATC NeoR/KanR 115 120 125 130 KVSIMADAMRRLHTLDPATC NeoR/KanR KVSIMADAMRRLHTLDPATC FYGDHSICHPPQMRKIRSGA a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g

3540 135 140 145 150 PFDHQAKHRIERARTRMEAG NeoR/KanR 135 140 145 150 PFDHQAKHRIERARTRMEAG NeoR/KanR PFDHQAKHRIERARTRMEAG WEVVLRFMADLSCTSPHFGT c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g

3600 155 160 165 170 LVDQDDLDEEHQGLAPAELF NeoR/KanR 155 160 165 170 LVDQDDLDEEHQGLAPAELF NeoR/KanR LVDQDDLDEEHQGLAPAELF KDILIIQVFLMLPERWGFQE t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c

3660 175 180 185 190 ARLKASMPDGEDLVVTHGDA NeoR/KanR 175 180 185 190 ARLKASMPDGEDLVVTHGDA NeoR/KanR ARLKASMPDGEDLVVTHGDA GPELRAHGVALIEDHGMAIG c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a

3720 195 200 205 210 CLPNIMVENGRFSGFIDCGR NeoR/KanR 195 200 205 210 CLPNIMVENGRFSGFIDCGR NeoR/KanR CLPNIMVENGRFSGFIDCGR AQRIDHHFIAAKRSEDVTAP g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g

RsrII 3780 215 220 225 230 LGVADRYQDIALATRDIAEE NeoR/KanR 215 220 225 230 LGVADRYQDIALATRDIAEE NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F LGVADRYQDIALATRDIAEE QTHRVAILVYRQSGTINSFL t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g

3840 235 240 245 250 LGGEWADRFLVLYGIAAPDS NeoR/KanR 235 240 245 250 LGGEWADRFLVLYGIAAPDS NeoR/KanR LGGEWADRFLVLYGIAAPDS KAAFPSVAEEHKVTDGSGIR t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g

BstBI 3900 255 260 265 QRIAFYRLLDEFF* NeoR/KanR 255 260 265 QRIAFYRLLDEFF* NeoR/KanR QRIAFYRLLDEFF* LADGEIAKKVLEESRSEPTR a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t

3960 MTDQATPNLPSRDFDSTAAF FSRGLSAWGAM a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a

PfoI 4020 YERLGFGIVFRDAGWMILQR c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c

4080 A C T T T G T G C C T T TK-pA-R GDLMLEFFAHPRGRLTETRK c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t

4140 HSV TK poly(A) signal C C T C T G T T TK-pA-R ETIPEGTRAMTAIKRQNKTH g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t

4200 HSV TK poly(A) signal GVGSFVHKRGVRSQGWHSVD c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t

4260 TPPRPHWGQYARVSSFSPPH t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t

4320 PPSSGEGPGLAANVGAAGPA c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g

4380 IASGYSYIL* c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t

4440 a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a

4500 ori t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a

4560 ori t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c

4620 ori t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t

4680 ori g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a

4740 ori a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t

4800 ori t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g

ApaLI 4860 ori g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a

4920 ori g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t

4980 ori G G G pBR322ori-F c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c

5040 ori A A A C G C C T G G T A T C T T T pBR322ori-F a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a

5100 ori t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a

PciI 5160 a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t

5184 t t c t g t g g a t a a c c g t a t t a c c g c a a g a c a c c t a t t g g c a t a a t g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.