Addgene HT041_FCMV_CheRiff_CFP - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: HT041_FCMV_CheRiff_CFP

Map View

pBR322ori-F (9665 .. 9684) L4440 (9414 .. 9431) CAP binding site lac promoter M13/pUC Reverse (9196 .. 9218) lac operator M13 Reverse (9183 .. 9199) M13 rev BstZ17I (9145) EBV-rev (9104 .. 9123) SV40pA-R (9050 .. 9069) FseI (8785) SgrAI (8623) MauBI (8545) AvrII (8380) StuI (8379) SV40pro-F (8308 .. 8327) pBABE 3' (8061 .. 8081) F1ori-F (7921 .. 7942) F1ori-R (7711 .. 7730) BGH-rev (7348 .. 7365) PmeI (7332) 5' LTR (truncated) BspEI (7129) KpnI (6755) Acc65I (6751) Bsu36I (6747) pBluescriptKS (6621 .. 6637) PaeR7I - XhoI (6633) SacII (6532) WPRE-R (6082 .. 6102) EcoRI (6005) BsrGI (5994) EGFP-C (5938 .. 5959) EXFP-R (5591 .. 5610) EGFP-N (5330 .. 5351) BsaBI * (5276) FspI (367) PvuI (515) ScaI (625) Amp-R (695 .. 714) SgrDI (1066) pRS-marker (1112 .. 1131) MluI (1296) SpeI (1317) CMV enhancer KasI (2083) NarI (2084) SfoI (2085) PluTI (2087) BbvCI (2866) KflI - PpuMI (3376) PstI (3479) PacI (3663) CMV promoter LNCX (4185 .. 4209) BsmBI - Esp3I (4209) XbaI (4259) BamHI (4265) BfuAI - BspMI (5067) HT041_FCMV_CheRiff_CFP 10,272 bp

Sequence View

5ʹ 3ʹ 60 t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a

120 285 280 275 *WHKILSAGIEAIQRN AmpR *WHKILSAGIEAIQRN t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a

180 270 265 260 255 REDMTAQSGTTYIVVIRSPK AmpR REDMTAQSGTTYIVVIRSPK c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t

240 250 245 240 235 GDPGLAAIIGRSGREGAGSK AmpR GDPGLAAIIGRSGREGAGSK c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t

300 230 225 220 215 DAIFWGAPLASRLLPGAVKD AmpR DAIFWGAPLASRLLPGAVKD t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g

360 210 205 200 195 AEMWDILQQRSALTLLEGTL AmpR AEMWDILQQRSALTLLEGTL g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a

FspI 420 190 185 180 175 LKRLTTAMAVPMTTDREDNP AmpR LKRLTTAMAVPMTTDREDNP a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a

480 170 165 160 155 IAENLEPEWRDLRTVHDGMN AmpR IAENLEPEWRDLRTVHDGMN a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c

PvuI 540 150 145 140 135 HLFATLEKPGGITTLLLNAA AmpR HLFATLEKPGGITTLLLNAA t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t

600 130 125 120 115 TNDSMTIAASCLERVTMGDT AmpR TNDSMTIAASCLERVTMGDT g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t

ScaI 660 110 105 100 95 LHKETVPSYEVLDNQSYHIR AmpR LHKETVPSYEVLDNQSYHIR a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c

720 90 85 80 75 RGLQEQGADIRSLVAGCLLV AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RGLQEQGADIRSLVAGCLLV c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a

780 70 65 60 55 KFTSMMPFREEPRFSELIKG AmpR KFTSMMPFREEPRFSELIKG t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c

840 50 45 40 35 SNLDLEIYGVRAGLQDEADK AmpR SNLDLEIYGVRAGLQDEADK c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a

900 30 25 VKVLTEPH 20 15 signal sequence AFVPLCFAAFFP AmpR VKVLTEPHAFVPLCFAAFFP a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t

960 10 5 1 signal sequence ILAVRFHQISM AmpR AmpR promoter ILAVRFHQISM a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g

1020 AmpR promoter a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t

SgrDI 1080 AmpR promoter c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t

1140 A T G T T A G A C G A G A C T A C G G C pRS-marker g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t

1200 g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a

1260 a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g

SpeIMluI 1320 CMV enhancer g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t

1380 CMV enhancer g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c

1440 CMV enhancer t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t

1500 CMV enhancer c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a

1560 CMV enhancer g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t

1620 CMV enhancer g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t

1680 CMV enhancer t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t

1740 CMV enhancer CMV promoter t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a

1800 CMV promoter t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c

1860 CMV promoter a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g

1920 CMV promoter 5' LTR (truncated) g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c

1980 5' LTR (truncated) a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g

2040 5' LTR (truncated) t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t

KasINarISfoIPluTI 2100 5' LTR (truncated) t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a

2160 HIV-1 Ψ t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g

2220 HIV-1 Ψ g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c

2280 HIV-1 Ψ g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g

2340 g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c

2400 t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c

2460 a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g

2520 t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t

2580 a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c

2640 MRDNWR c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t

2700 SELYKYKVVKIEPLGVAPTK a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c

2760 RRE AKRRVVQREKRAVGIGALFL g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a

2820 RRE GFLGAAGSTMGAASMTLTVQ g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c

BbvCI 2880 RRE ARQLLSGIVQQQNNLLRAIE g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c

2940 RRE AQQHLLQLTVWGIKQLQARI g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g

3000 RRE LAVERYLKDQQLLGIWGCSG c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t

3060 KLICTTAVPWNASWSNKSLE a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t

3120 QIWNHTTWMEWDREINNYTS c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g

3180 1 5 KNEQEL gp41 peptide LIHSLIEESQNQQEKNEQEL t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t

3240 10 15 LELDKWASL (in frame with gp41 peptide) WNWFNITNWLW gp41 peptide LELDKWASLWNWFNITNWLW t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c

3300 (in frame with gp41 peptide) YIKLFIMIVGGLVGLRIVFA YIKLFIMIVGGLVGLRIVFA t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a

3360 (in frame with gp41 peptide) VLSIVNRVRQGYSPLSFQTH VLSIVNRVRQGYSPLSFQTH g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g

PpuMIKflI 3420 (in frame with gp41 peptide) LPTPRGPDRPEGIEEEGGER LPTPRGPDRPEGIEEEGGER c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t

PstI 3480 (in frame with gp41 peptide) DRDRSIRLVNGSALRAPILQ DRDRSIRLVNGSALRAPILQ g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g c g c c a a t t c t g c a g c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c g c g g t t a a g a c g t c

3540 (in frame with gp41 peptide) TNGSIHPQF* cPPT/CTS TNGSIHPQF* a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g

3600 cPPT/CTS a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t

3660 cPPT/CTS a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g t t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c a a

PacI 3720 CMV enhancer a a t t a a g c t t g g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g t t a a t t c g a a c c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c

3780 CMV enhancer c c c a a c g a c c c c c g c c c a t t g a c g a c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c t g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t

3840 CMV enhancer g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t

3900 CMV enhancer c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g

3960 CMV enhancer g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g

4020 CMV enhancer CMV promoter g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t

4080 CMV promoter *HSNT t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c

4140 CMV promoter KAGFDVPSELIDYCSRGMSA t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c

4200 CMV promoter A G C T C G T T T A G T G A A C LNCX FPRYAHVTPPRYLCLENLSG c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g

XbaIBsmBIEsp3I 4260 C G T C A G A T C LNCX DSRRSVGDVSNQGGYFVGVR c g t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g a c t c g c a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c t g a g

BamHI 4320 1 5 10 15 MGGAPAPDAHSAPPGND CheRiff MGGAPAPDAHSAPPGND SSGHASSRSWVSVAGWRSVI t a g a g g a t c c a t g g g c g g a g c t c c t g c t c c a g a c g c t c a c a g c g c c c c a c c t g g a a a c g a a t c t c c t a g g t a c c c g c c t c g a g g a c g a g g t c t g c g a g t g t c g c g g g g t g g a c c t t t g c t

4380 20 25 30 35 SAGGSEYHAPAGYQVNPPYH CheRiff SAGGSEYHAPAGYQVNPPYH RGSATLVMGWSSILHIWGVV t t c t g c c g g a g g c a g t g a g t a c c a t g c c c c a g c t g g a t a t c a a g t g a a t c c a c c c t a c c a a a g a c g g c c t c c g t c a c t c a t g g t a c g g g g t c g a c c t a t a g t t c a c t t a g g t g g g a t g g t

4440 40 45 50 55 PVHGYEEQCSSIYIYYGALW CheRiff PVHGYEEQCSSIYIYYGALW GHMPILFLAAGDVDVIPGQP c c c c g t g c a t g g g t a t g a g g a a c a g t g c a g c t c c a t c t a c a t c t a c t a t g g g g c c c t g t g g g g g c a c g t a c c c a t a c t c c t t g t c a c g t c g a g g t a g a t g t a g a t g a t a c c c c g g g a c a c

4500 60 65 70 75 EQETARGFQWFAVFLSALFL CheRiff EQETARGFQWFAVFLSALFL LLFCSPAELPKGHEQRGQKQ g g a g c a g g a a a c a g c t a g g g g c t t c c a g t g g t t t g c c g t g t t c c t g t c t g c c c t g t t t c t c c t c g t c c t t t g t c g a t c c c c g a a g g t c a c c a a a c g g c a c a a g g a c a g a c g g g a c a a a g a

4560 80 85 90 95 AFYGWHAYKASVGWEEVYVC CheRiff AFYGWHAYKASVGWEEVYVC SEVAPVGILGAHSPLFHVHA g g c t t t c t a c g g c t g g c a c g c c t a t a a g g c c a g c g t g g g a t g g g a g g a a g t g t a c g t g t g c c g a a a g a t g c c g a c c g t g c g g a t a t t c c g g t c g c a c c c t a c c c t c c t t c a c a t g c a c a c

4620 100 105 110 115 SVELIKVILEIYFEFTSPAM CheRiff SVELIKVILEIYFEFTSPAM GHLQDFHNQLDIELEGTRSH c t c c g t g g a g c t g a t c a a a g t g a t t c t g g a g a t c t a t t t c g a g t t c a c c a g t c c t g c t a t g a g g c a c c t c g a c t a g t t t c a c t a a g a c c t c t a g a t a a a g c t c a a g t g g t c a g g a c g a t a

4680 120 125 130 135 LFLYGGNITPWLRYAEWLLT CheRiff LFLYGGNITPWLRYAEWLLT QEQVSPVNGWPQSIGFPQQC g c t g t t c c t g t a c g g a g g g a a c a t t a c c c c a t g g c t g a g a t a t g c c g a a t g g c t g c t g a c c g a c a a g g a c a t g c c t c c c t t g t a a t g g g g t a c c g a c t c t a t a c g g c t t a c c g a c g a c t g

4740 140 145 150 155 CPVILIHLSNITGLSEAYNK CheRiff CPVILIHLSNITGLSEAYNK TGHDQNMQRVDGAQTLCVIL a t g t c c c g t g a t c c t g a t t c a t c t g t c t a a c a t c a c c g g c c t g a g t g a g g c a t a c a a t a a t a c a g g g c a c t a g g a c t a a g t a g a c a g a t t g t a g t g g c c g g a c t c a c t c c g t a t g t t a t t

4800 160 165 170 175 RTMALLVSDLGTICMGVTAA CheRiff RTMALLVSDLGTICMGVTAA PCHSQQHGVQSSNAHSHCGS g c g g a c a a t g g c t c t g c t g g t g t c c g a c c t g g g a a c t a t t t g c a t g g g a g t g a c a g c c g c c g c c t g t t a c c g a g a c g a c c a c a g g c t g g a c c c t t g a t a a a c g t a c c c t c a c t g t c g g c g

4860 180 185 190 195 LATGWVKWLFYCIGLVYGTQ CheRiff LATGWVKWLFYCIGLVYGTQ QGSPPHLPQKVTDAQHISGL t c t g g c c a c t g g g t g g g t g a a g t g g c t g t t t t a c t g t a t c g g c c t g g t g t a t g g a a c c c a a g a c c g g t g a c c c a c c c a c t t c a c c g a c a a a a t g a c a t a g c c g g a c c a c a t a c c t t g g g t

4920 200 205 210 215 TFYNAGIIYVESYYIMPAGG CheRiff TFYNAGIIYVESYYIMPAGG CEVVSSDDVHLRVIDHRGAA g a c a t t c t a c a a c g c t g g a a t c a t c t a c g t g g a g t c t t a c t a t a t c a t g c c t g c c g g c g g c t g t a a g a t g t t g c g a c c t t a g t a g a t g c a c c t c a g a a t g a t a t a g t a c g g a c g g c c g c c

4980 220 225 230 235 CKKLVLAMTAVYYSSWLMFP CheRiff CKKLVLAMTAVYYSSWLMFP TLFQHQGHSGHVIRTPQHKG c t g t a a g a a a c t g g t g c t g g c c a t g a c t g c c g t g t a c t a t t c t a g t t g g c t g a t g t t t c c g a c a t t c t t t g a c c a c g a c c g g t a c t g a c g g c a c a t g a t a a g a t c a a c c g a c t a c a a a g g

5040 240 245 250 255 GLFIFGPEGMHTLSVAGSTI CheRiff GLFIFGPEGMHTLSVAGSTI AQEDKPRFAHVGQAHSPGSN c g g c c t g t t c a t c t t t g g g c c t g a a g g c a t g c a c a c c c t g a g c g t g g c t g g g t c c a c t a t g c c g g a c a a g t a g a a a c c c g g a c t t c c g t a c g t g t g g g a c t c g c a c c g a c c c a g g t g a t a

BspMIBfuAI 5100 260 265 270 275 GHTIADLLSKNIWGLLGHFL CheRiff GHTIADLLSKNIWGLLGHFL AMGDGVQQGLINPSQQPVEQ t g g c c a t a c c a t c g c c g a c c t g c t g t c c a a g a a t a t t t g g g g a c t g c t g g g g c a c t t c c t a c c g g t a t g g t a g c g g c t g g a c g a c a g g t t c t t a t a a a c c c c t g a c g a c c c c g t g a a g g a

5160 280 285 290 295 RIKIHEHIIMYGDIRRPVSS CheRiff RIKIHEHIIMYGDIRRPVSS PDFNVLM g c g g a t c a a a a t t c a c g a g c a t a t c a t t a t g t a c g g c g a t a t c a g g a g a c c a g t g a g c t c c g c c t a g t t t t a a g t g c t c g t a t a g t a a t a c a t g c c g c t a t a g t c c t c t g g t c a c t c g a g

5220 300 305 310 315 QFLGRKVDVLAFVTEEDKV A CheRiff QFLGRKVDVLAFVTEEDKVA c c a g t t t c t g g g a c g c a a g g t g g a c g t g c t g g c c t t c g t g a c a g a g g a a g a t a a a g t g g c g g t c a a a g a c c c t g c g t t c c a c c t g c a c g a c c g g a a g c a c t g t c t c c t t c t a t t t c a c c g

BsaBI* 5280 (in frame with CheRiff) AAKSRITSEGEYIPLDQIDI AAKSRITSEGEYIPLDQIDI g g c c g c c a a g a g c a g g a t c a c c a g c g a g g g c g a g t a c a t c c c c c t g g a c c a g a t c g a c a t c c g g c g g t t c t c g t c c t a g t g g t c g c t c c c g c t c a t g t a g g g g g a c c t g g t c t a g c t g t a

5340 NV 1a V 5 10 15 SKGEELFTGVVPILVEL CFP G A C C A G C T C G A EGFP-N NVVSKGEELFTGVVPILVEL c a a c g t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g t t g c a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a

5400 20 25 30 35 DGDVNGHRFSVSGEGEGDAT CFP C C T G C C G C T G C EGFP-N DGDVNGHRFSVSGEGEGDAT g g a c g g c g a c g t a a a c g g c c a c a g g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c c t g c c g c t g c a t t t g c c g g t g t c c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g

5460 40 45 50 55 YGKLTLKFICTTGKLPVPWP CFP YGKLTLKFICTTGKLPVPWP c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g

5520 60 65 70 75 TLVTTLTWGVQCFSRYPDHM CFP TLVTTLTWGVQCFSRYPDHM c a c c c t c g t g a c c a c c c t g a c c t g g g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g t g g g a g c a c t g g t g g g a c t g g a c c c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a

5580 80 85 90 95 KQHDFFKSAMPEGYVQERTI CFP KQHDFFKSAMPEGYVQERTI g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g t a c c a t c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c a t g g t a

5640 100 105 110 115 FFKDDGNYKTRAEVKFEGDT CFP C T G C T G C C G T T G A T G T T C T G EXFP-R FFKDDGNYKTRAEVKFEGDT c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g

5700 120 125 130 135 LVNRIELKGIDFKEDGNILG CFP LVNRIELKGIDFKEDGNILG c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c

5760 140 145 150 155 HKLEYNYISHNVYITADKQK CFP HKLEYNYISHNVYITADKQK g c a c a a g c t g g a g t a c a a c t a c a t c a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a c g t g t t c g a c c t c a t g t t g a t g t a g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t

5820 160 165 170 175 NGIKAHFKIRHNIEDGSVQL CFP NGIKAHFKIRHNIEDGSVQL g a a c g g c a t c a a g g c c c a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c t t g c c g t a g t t c c g g g t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a

5880 180 185 190 195 ADHYQQNTPIGDGPVLLPDN CFP ADHYQQNTPIGDGPVLLPDN c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t

5940 200 205 210 215 HYLSTQSALSKDPNEKRDHM CFP C A T EGFP-C HYLSTQSALSKDPNEKRDHM c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a

BsrGI 6000 220 225 230 235 VLLEFVTAAGITLGMDELYK CFP G G T C C T G C T G G A G T T C G T G EGFP-C VLLEFVTAAGITLGMDELYK g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t

EcoRI 6060 * CFP WPRE * g t a a g a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g c a t t c t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c

6120 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R M a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a

6180 WPRE PLYHAIASRMAFIFSSLYKS MLLLPVWLSFSPPCIN g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g

6240 WPRE WLLSLYEELWPVVRQRGVVC PGCCLFMRSCGPLSGNVAWC c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c

6300 WPRE TVFADATPTGWGIATTCQLL ALCLLTQPPLVGALPPPVSS c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a

6360 WPRE SGTFAFPLPIATAELIAACL FPGLSLSPSLLPRRNSSPPA t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a

6420 WPRE ARCWTGARLLGTDNSVVLSG LPAAGQGLGCWALTIPWCCR t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c

6480 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPNQAPR KSSSFPWLLACVATWILRGT GNHRPFLGCSPVLPPGFCAG g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g

SacII 6540 WPRE (in frame with Factor Xa site) GEAVDR 1 RGEI Factor Xa site SFCYVPSALNPADLPSRGLL RPSATSLRPSIQRTFLPAAC g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a

6600 WPRE PALRPLPRLRLRPQTSRISL CRLCGLFRVFAFALRRVGSP g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a

PaeR7IXhoI 6660 WPRE KS primer C T A T G G C A G C T G G A G C T pBluescriptKS WAASPHRYRRPRDLEKHGAI FGPPPRIDTVDLET* t t g g g c c g c c t c c c c g c a t c g a t a c c g t c g a c c t c g a g a c c t a g a a a a a c a t g g a g c a a t a a c c c g g c g g a g g g g c g t a g c t a t g g c a g c t g g a g c t c t g g a t c t t t t t g t a c c t c g t t a

6720 TSSNTAATNADCAWLEAQEE c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c a a g a g g a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c g t g t t c t c c t

Bsu36IAcc65IKpnI 6780 EEVGFPVTPQVPLRPMTYKA g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g

6840 AVDLSHFLKEKGGLEGLIHS a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g

6900 QRRQDILDLWIYHTQGYFPD c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t t c c c t g a g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g a a g g g a c t

6960 WQNYTPGPGIRYPLTFGWCY t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t c c a c t g a c c t t t g g a t g g t g c t a a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a g g t g a c t g g a a a c c t a c c a c g a t

7020 KLVPVEQEKVEEANEGENTR c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g c c a a t g a a g g a g a g a a c a c c c g g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t c g g t t a c t t c c t c t c t t g t g g g c

7080 LLHPVSLHGMDDPEREVLEW c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t a t t a g a g t g g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c a t a a t c t c a c

BspEI 7140 RFDSRLAFHHMARELHPDCT g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c c g a g a g c t g c a t c c g g a c t g t a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g g c t c t c g a c g t a g g c c t g a c a t g

7200 5' LTR (truncated) GSLWLDQI* t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g

7260 5' LTR (truncated) a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a

7320 5' LTR (truncated) g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c

PmeI 7380 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c

7440 bGH poly(A) signal t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a

7500 bGH poly(A) signal c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c

7560 bGH poly(A) signal g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c

7620 bGH poly(A) signal a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g

7680 f1 ori c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t

7740 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c

7800 f1 ori c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a

7860 f1 ori t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c

7920 f1 ori g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t

7980 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a

8040 f1 ori t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a

8100 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g

8160 SV40 promoter c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t

8220 SV40 promoter g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g

8280 SV40 promoter SV40 ori c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g

8340 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g

StuIAvrII 8400 SV40 promoter SV40 ori t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a

8460 EM7 promoter c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c

8520 1 MAKL EM7 promoter BleoR g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g t t g c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c a a c

MauBI 8580 5 10 15 20 TSAVPVLTARDVAGAVEFWT BleoR a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g

SgrAI 8640 25 30 35 40 DRLGFSRDFVEDDFAGVVRD BleoR g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g

8700 45 50 55 60 DVTLFISAVQDQVVPDNTLA BleoR g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g

8760 65 70 75 80 WVWVRGLDELYAEWSEVVST BleoR t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c

FseI 8820 85 90 95 100 NFRDASGPAMTEIGEQPWGR BleoR a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c

8880 105 110 115 120 EFALRDPAGNCVHFVAEEQD BleoR g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g

8940 125 * BleoR t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t

9000 a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g

9060 SV40 poly(A) signal C G T T A T C G T A G SV40pA-R t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g

9120 SV40 poly(A) signal G T G G T T T G T C C A A A C T C EBV-rev T G T T T A A A G SV40pA-R a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g

BstZ17I 9180 SV40 poly(A) signal A T C EBV-rev a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t

9240 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t

9300 -35 lac promoter CAP binding site g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a

9360 g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t

9420 G A G C G A G L4440 a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g

9480 T G A C T G A G C G A L4440 a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c

9540 g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g

9600 ori c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g

9660 ori c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t

9720 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g

9780 ori c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a

9840 ori a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c

9900 ori c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g

9960 ori a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t

10,020 ori g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a

10,080 ori a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a

10,140 ori g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c

10,200 ori c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c

10,260 t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t

3ʹ 5ʹ 10,272 a g g a t c t t c a c c t c c t a g a a g t g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.