Addgene pDONR221_SLC4A2 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pDONR221_SLC4A2 Sequencing Result


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PciI (6267) pBR322ori-F (6112 .. 6131) BciVI (6069) ApaLI (5953) BsmBI - Esp3I (5108) AsiSI - PvuI (5092) SspI (5017) Kan-R (4734 .. 4753) NruI (4749) pENTR-R (4656 .. 4675) M13 Reverse (4538 .. 4554) T7 (4514 .. 4533) T7 promoter EcoRV (4510) BstXI (4393) FspAI (4148) BbvCI (4001) RsrII (3852) SphI (3748) XcmI (3508) BtgZI (3481) BspEI * (3465) BamHI (3462) AgeI (3318) L4440 (92 .. 109) BspQI - SapI (111) rrnB T2 terminator AclI (364) HincII - HpaI (501) M13/pUC Forward (522 .. 544) M13 Forward (536 .. 553) M13 fwd AhdI (549) AflII (554) Eco53kI (678) SacI (680) PasI (796) AccI (1112) AleI (1256) PshAI (1363) HindIII (3109) pDONR221_SLC4A2 6273 bp

Sequence View

5ʹ 3ʹ 60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t

BspQISapI 120 A G C G A G T C A G T G A G C G A G L4440 t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

180 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t

240 c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g

300 rrnB T2 terminator t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t

360 g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g

AclI 420 rrnB T1 terminator a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t

480 rrnB T1 terminator c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c

HincIIHpaI 540 M13 fwd C C C A G T C A C G A C G T T G T A A M13/pUC Forward T G T A A M13 Forward g c a g t t c c c t a c t c t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a c g t c a a g g g a t g a g a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t

AflIIAhdI 600 M13 fwd attL1 A A C G M13/pUC Forward A A C G A C G G C C A G T M13 Forward *KSQYHG a a c g a c g g c c a g t c t t a a g c t c g g g c c c c a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c t t g c t g c c g g t c a g a a t t c g a g c c c g g g g t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g

660 attL1 MPTLYKK TRQLVNILLAKKYHWSQVFF c t g t t c g t t g c a a c a c a t t g a t g a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a g a c a a g c a a c g t t g t g t a a c t a c t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t

Eco53kISacI 720 attL1 AGSTMSSAPRRPAKGADSFC CAGGHAGGWPSGCLSCIREA a g c a g g c t c c a c c a t g a g c t c c g c c c c a c g g a g a c c c g c a a a g g g a g c a g a t t c t t t c t g t c g t c c g a g g t g g t a c t c g a g g c g g g g t g c c t c t g g g c g t t t c c c t c g t c t a a g a a a g a c

780 TPEPESLGPGTPGFPEQEED CWLWLAQSRSGWSKWFLLLV c a c a c c a g a g c c a g a g a g c c t g g g a c c t g g a a c c c c a g g a t t t c c a g a a c a g g a g g a g g a g t g t g g t c t c g g t c t c t c g g a c c c t g g a c c t t g g g g t c c t a a a g g t c t t g t c c t c c t c c t

PasI 840 ELHRTLGVERFEEILQEAGS LQVAGQSHLPELLDQLLCSA c g a g c t g c a c c g c a c c c t g g g a g t g g a g c g g t t c g a g g a g a t c c t g c a g g a g g c a g g a a g g c t c g a c g t g g c g t g g g a c c c t c a c c t c g c c a a g c t c c t c t a g g a c g t c c t c c g t c c t t c

900 RGGEEPGRSYGEEDFEYHRQ SSSLLWAPRVSLLIKLIVSL c a g a g g a g g a g a g g a g c c a g g c a g g t c t t a c g g a g a g g a g g a t t t t g a g t a t c a c a g a c a g t c t c c t c c t c t c c t c g g t c c g t c c a g a a t g c c t c t c c t c c t a a a a c t c a t a g t g t c t g t

960 SSHHIHHPLSTHLPPDARRR RAVVDVVWQACVQWRVCPAP g t c t a g c c a c c a c a t c c a c c a c c c a c t g a g c a c a c a c c t g c c a c c t g a c g c a a g g c g c c g c a g a t c g g t g g t g t a g g t g g t g g g t g a c t c g t g t g t g g a c g g t g g a c t g c g t t c c g c g g c

1020 KTPQGPGRKPRRRPGASPTG LGRLAWSPLWSPAGSCRRGS g a a g a c c c c t c a g g g c c c a g g a a g g a a g c c a a g a a g g c g c c c c g g a g c a t c t c c t a c c g g c t t c t g g g g a g t c c c g g g t c c t t c c t t c g g t t c t t c c g c g g g g c c t c g t a g a g g a t g g c c

1080 ETPTIEEGEEDEDEASEAEG LGWCDLLSLLVLILCALCLS a g a g a c c c c a a c a a t c g a g g a g g g a g a g g a g g a c g a g g a t g a g g c a a g c g a g g c a g a g g g t c t c t g g g g t t g t t a g c t c c t c c c t c t c c t c c t g c t c c t a c t c c g t t c g c t c c g t c t c c c

AccI 1140 ARALTQPSPVSTPSSVQFFL CSGQGLRGWHRCRGRHLEKQ a g c a a g a g c c c t g a c c c a g c c t t c c c c a g t g t c t a c a c c t t c c t c t g t g c a g t t c t t t c t t c g t t c t c g g g a c t g g g t c g g a a g g g g t c a c a g a t g t g g a a g g a g a c a c g t c a a g a a a g a

1200 QEDDSADRKAERTSPSSPAP LLVIAGVSLGLPCGWAGWCR g c a g g a g g a c g a t a g c g c c g a c a g a a a g g c c g a g a g g a c a t c c c c a a g c t c c c c a g c a c c c g t c c t c c t g c t a t c g c g g c t g t c t t t c c g g c t c t c c t g t a g g g g t t c g a g g g g t c g t g g

AleI 1260 LPHQEATPRASKGAQAGTQV QWVLLGCRPGALAGLGAGLH t c t g c c a c a c c a g g a g g c c a c a c c t a g g g c c a g c a a g g g c g c c c a g g c c g g c a c c c a g g t a g a c g g t g t g g t c c t c c g g t g t g g a t c c c g g t c g t t c c c g c g g g t c c g g c c g t g g g t c c a

1320 EEAEAEAVAVASGTAGGDDG LLGLGLCHCHCASGCAAVIA g g a g g a g g c c g a g g c c g a g g c a g t g g c a g t g g c a a g c g g a a c c g c a g g c g g c g a c g a t g g c c t c c t c c g g c t c c g g c t c c g t c a c c g t c a c c g t t c g c c t t g g c g t c c g c c g c t g c t a c c

PshAI 1380 GASGRPLPKAQPGHRSYNLQ AGGASGQRLCLRSVPRVVQL c g g c g c c t c c g g c a g a c c c c t g c c t a a g g c a c a g c c t g g a c a c a g g t c t t a c a a c c t g c a g c c g c g g a g g c c g t c t g g g g a c g g a t t c c g t g t c g g a c c t g t g t c c a g a a t g t t g g a c g t

1440 ERRRIGSMTGAEQALLPRVP LPSPDSAHCSCLLGQQWSHR g g a g c g g a g a a g g a t c g g a a g c a t g a c a g g a g c a g a g c a g g c c c t g c t g c c a a g a g t g c c c c t c g c c t c t t c c t a g c c t t c g t a c t g t c c t c g t c t c g t c c g g g a c g a c g g t t c t c a c g g

1500 TDEIEAQTLATADLDLMKSH GILDLCLGQCCCVQIQHLGV t a c c g a t g a g a t c g a g g c a c a g a c c c t g g c a a c a g c a g a c c t g g a t c t g a t g a a g t c c c a a t g g c t a c t c t a g c t c c g t g t c t g g g a c c g t t g t c g t c t g g a c c t a g a c t a c t t c a g g g t

1560 RFEDVPGVRRHLVRKNAKGS SELVHWAHAPVQHPLIGLAA c a g a t t c g a g g a c g t g c c a g g c g t g c g c c g g c a c c t g g t g a g g a a g a a t g c c a a g g g c a g g t c t a a g c t c c t g c a c g g t c c g c a c g c g g c c g t g g a c c a c t c c t t c t t a c g g t t c c c g t c

1620 TQSGREGREPGPTPRARPRA CLGASLAPLWSWGRPCPWPC c a c a c a g t c c g g c a g a g a g g g c a g g g a g c c a g g a c c a a c c c c t a g g g c a a g g c c a a g g g c g t g t g t c a g g c c g t c t c t c c c g t c c c t c g g t c c t g g t t g g g g a t c c c g t t c c g g t t c c c g

1680 PHKPHEVFVELNELLLDKNQ GVLRVLHEHLQVLQQQILIL a c c c c a c a a g c c t c a c g a g g t g t t c g t g g a g c t g a a c g a g c t g c t g c t g g a t a a g a a t c a t g g g g t g t t c g g a g t g c t c c a c a a g c a c c t c g a c t t g c t c g a c g a c g a c c t a t t c t t a g t

1740 EPQWRETARWIKFEEDVEEE LRLPPLCCPPDLELLVHLLL g g a g c c t c a g t g g a g g g a g a c a g c a a g g t g g a t c a a g t t c g a g g a g g a c g t g g a g g a g g a c c t c g g a g t c a c c t c c c t c t g t c g t t c c a c c t a g t t c a a g c t c c t c c t g c a c c t c c t c c t

1800 TERWGKPHVASLSFRSLLEL GLPPALWVHGRQAKPAQQLQ g a c c g a g c g g t g g g g c a a g c c a c a c g t g g c c t c t c t g a g c t t t c g g a g c c t g c t g g a g c t c t g g c t c g c c a c c c c g t t c g g t g t g c a c c g g a g a g a c t c g a a a g c c t c g g a c g a c c t c g a

1860 RRTLAHGAVLLDLDQQTLPG SPCQCVSGHQQVQILLGQWS g a g a a g g a c a c t g g c a c a c g g a g c c g t g c t g c t g g a c c t g g a t c a g c a g a c c c t g c c a g g c t c t t c c t g t g a c c g t g t g c c t c g g c a c g a c g a c c t g g a c c t a g t c g t c t g g g a c g g t c c

1920 VAHQVVEQMVISDQIKAEDR HCVLHHLLHDDGILDLCLVP a g t g g c a c a c c a g g t g g t g g a g c a g a t g g t c a t c t c c g a t c a g a t c a a g g c a g a g g a c a g t c a c c g t g t g g t c c a c c a c c t c g t c t a c c a g t a g a g g c t a g t c t a g t t c c g t c t c c t g t c

1980 ANVLRALLLKHSHPSDEKDF CVHQPGQQQLVRVGAVLLIE g g c a a a c g t g c t g c g g g c c c t g c t g c t g a a g c a c t c t c a c c c c a g c g a c g a g a a g g a t t t c c g t t t g c a c g a c g c c c g g g a c g a c g a c t t c g t g a g a g t g g g g t c g c t g c t c t t c c t a a a

2040 SFPRNISAGSLGSLLGHHHG RKRPIDGGSRQSAQQSVVVS c t c t t t t c c t c g g a a t a t c t c c g c c g g a t c t c t g g g a a g c c t g c t g g g a c a c c a c c a c g g g a g a a a a g g a g c c t t a t a g a g g c g g c c t a g a g a c c c t t c g g a c g a c c c t g t g g t g g t g c c

2100 QGAESDPHVTEPLMGGVPET LSGLAIWVHCLWQHSSHRLG a c a g g g a g c c g a g a g c g a t c c a c a c g t g a c a g a g c c a c t g a t g g g a g g a g t g c c t g a g a c t g t c c c t c g g c t c t c g c t a g g t g t g c a c t g t c t c g g t g a c t a c c c t c c t c a c g g a c t c t g

2160 RLEVERERELPPPAPPAGIT AQLHLSLPLQWWRCWWCADG c c g c c t g g a g g t g g a g a g a g a g a g g g a g c t g c c a c c a c c t g c a c c a c c a g c a g g c a t c a c g g c g g a c c t c c a c c t c t c t c t c t c c c t c g a c g g t g g t g g a c g t g g t g g t c g t c c g t a g t g

2220 RSKSKHELKLLEKIPENAEA PGLRLVLQLQQLLDGLVCLC c c g g t c c a a g t c t a a g c a c g a g c t g a a g c t g c t g g a g a a g a t c c c c g a g a a c g c a g a g g c g g c c a g g t t c a g a t t c g t g c t c g a c t t c g a c g a c c t c t t c t a g g g g c t c t t g c g t c t c c g

2280 TVVLVGCVEFLSRPTMAFVR CHHQHSAHLEQAPRGHGKHA a a c a g t g g t g c t g g t g g g a t g c g t g g a g t t c c t g a g c a g g c c t a c c a t g g c c t t t g t g c g t t g t c a c c a c g a c c a c c c t a c g c a c c t c a a g g a c t c g t c c g g a t g g t a c c g g a a a c a c g c

2340 LREAVELDAVLEVPVPVRFL QPLCHLQIGHQLHWHGHSEQ c c t g a g g g a g g c a g t g g a g c t g g a t g c c g t g c t g g a g g t g c c a g t g c c c g t g a g a t t c c t g g a c t c c c t c c g t c a c c t c g a c c t a c g g c a c g a c c t c c a c g g t c a c g g g c a c t c t a a g g a

2400 FLLLGPSSANMDYHEIGRSI KQQQAGRAGVHIVVLDAPAD g t t t c t g c t g c t g g g c c c c t c t a g c g c c a a c a t g g a t t a c c a c g a g a t c g g c a g g a g c a t c a a a g a c g a c g a c c c g g g g a g a t c g c g g t t g t a c c t a a t g g t g c t c t a g c c g t c c t c g t a

2460 STLMSDKQFHEAAYLADERE GCQHGVLLEVLGGIQCILPL c t c c a c a c t g a t g t c c g a c a a g c a g t t c c a c g a g g c c g c c t a t c t g g c a g a t g a g a g g g a g a g g t g t g a c t a c a g g c t g t t c g t c a a g g t g c t c c g g c g g a t a g a c c g t c t a c t c t c c c t

2520 DLLTAINAFLDCSVVLPPSE VQQGGDIGKQVTGHHQRWRL g g a c c t g c t g a c c g c c a t c a a t g c c t t t c t g g a c t g t t c c g t g g t g c t g c c t c c a t c t g a c c t g g a c g a c t g g c g g t a g t t a c g g a a a g a c c t g a c a a g g c a c c a c g a c g g a g g t a g a c t

2580 VQGEELLRSVAHFQRQMLKK HLSLLQQAAHCVELPLHQLL g g t g c a g g g a g a g g a g c t g c t g c g c a g c g t g g c a c a c t t c c a g c g g c a g a t g c t g a a g a a c c a c g t c c c t c t c c t c g a c g a c g c g t c g c a c c g t g t g a a g g t c g c c g t c t a c g a c t t c t t

2640 REEQGRLLPTGAGLEPKSAQ SLLLAPQQRCSCSQLWLGGL g a g a g a g g a g c a g g g c a g g c t g c t g c c t a c a g g a g c a g g a c t g g a g c c a a a g t c c g c c c a c t c t c t c c t c g t c c c g t c c g a c g a c g g a t g t c c t c g t c c t g a c c t c g g t t t c a g g c g g g t

2700 DKALLQMVEAAGAAEDDPLR ILGQQLHHLCCSCCLVIWQP g g a t a a g g c c c t g c t g c a g a t g g t g g a g g c a g c a g g a g c a g c a g a g g a c g a t c c a c t g a g c c t a t t c c g g g a c g a c g t c t a c c a c c t c c g t c g t c c t c g t c g t c t c c t g c t a g g t g a c t c

2760 RTGRPFGGLIRDVRRRYPHY PGASREAAQDPVHSPAVRVI g a g g a c c g g c a g a c c t t t c g g c g g c c t g a t c a g g g a c g t g a g a a g g c g c t a c c c t c a c t a c t c c t g g c c g t c t g g a a a g c c g c c g g a c t a g t c c c t g c a c t c t t c c g c g a t g g g a g t g a t

2820 LSDFRDALDPQCLAAVIFIY QAVKSICQVRLAQCGHDEDV t c t g a g c g a c t t t a g a g a t g c a c t g g a c c c t c a g t g c c t g g c a g c c g t g a t c t t c a t c t a a g a c t c g c t g a a a t c t c t a c g t g a c c t g g g a g t c a c g g a c c g t c g g c a c t a g a a g t a g a t

2880 FAALSPAITFGGLLGEKTQD KGGQGWGDCEASQQSLLGLV c t t t g c c g c c c t g t c c c c a g c c a t c a c a t t c g g c g g a c t g c t g g g a g a g a a g a c c c a g g a g a a a c g g c g g g a c a g g g g t c g g t a g t g t a a g c c g c c t g a c g a c c c t c t c t t c t g g g t c c t

2940 LIGVSELIMSTALQGVVFCL QDAHALQDHGGGQLAHHKAQ c c t g a t c g g c g t g a g c g a g c t g a t c a t g t c c a c c g c c c t g c a g g g c g t g g t g t t t t g c c t g g a c t a g c c g c a c t c g c t c g a c t a g t a c a g g t g g c g g g a c g t c c c g c a c c a c a a a a c g g a

3000 LGAQPLLVIGFSGPLLVFEE QSCLWQQDDAKASWQQHELL g c t g g g a g c a c a g c c a c t g c t g g t c a t c g g c t t t a g c g g a c c a c t g c t g g t g t t c g a g g a c g a c c c t c g t g t c g g t g a c g a c c a g t a g c c g a a a t c g c c t g g t g a c g a c c a c a a g c t c c t

3060 AFFSFCSSNHLEYLVGRVWI GEKGKTGRIVQLIQHAPHPD g g c c t t c t t t t c c t t t t g t t c c t c t a a t c a c c t g g a g t a t c t g g t g g g c a g g g t g t g g a t c c g g a a g a a a a g g a a a a c a a g g a g a t t a g t g g a c c t c a t a g a c c a c c c g t c c c a c a c c t a

HindIII 3120 GFWLVFLALLMVALEGSFLV AEPQHKQGQQHHGQLSAEQH c g g c t t c t g g c t g g t g t t t c t g g c c c t g c t g a t g g t g g c c c t g g a g g g a a g c t t c c t g g t g c c g a a g a c c g a c c a c a a a g a c c g g g a c g a c t a c c a c c g g g a c c t c c c t t c g a a g g a c c a

3180 RFVSRFTQEIFAFLISLIFI SEHAPECLLDEGKQDAQDKD g a g a t t c g t g a g c a g g t t c a c a c a g g a g a t c t t c g c c t t t c t g a t c a g c c t g a t c t t t a t c t c t a a g c a c t c g t c c a a g t g t g t c c t c t a g a a g c g g a a a g a c t a g t c g g a c t a g a a a t a

3240 YETFYKLVKIFQEHPLHGCS VLGEILQHLDELLVRQVATR c t a c g a g a c c t t c t a t a a g c t g g t g a a g a t c t t c c a g g a g c a c c c t c t g c a c g g c t g t t c g a t g c t c t g g a a g a t a t t c g a c c a c t t c t a g a a g g t c c t c g t g g g a g a c g t g c c g a c a a g

3300 ASNSSEVDGGENMTWAGARP GAVAGLHISSLIHGPCSCPW t g c c a g c a a c a g c t c c g a g g t g g a t g g a g g a g a g a a t a t g a c c t g g g c a g g a g c a a g g c c a c g g t c g t t g t c g a g g c t c c a c c t a c c t c c t c t c t t a t a c t g g a c c c g t c c t c g t t c c g g

AgeI 3360 TLGPGNRSLAGQSGQGKPRG CQSWSVPRQCSLASLALRSS a a c a c t g g g a c c a g g a a a c c g g t c t c t g g c a g g a c a g a g c g g a c a g g g c a a g c c t a g a g g t t g t g a c c c t g g t c c t t t g g c c a g a g a c c g t c c t g t c t c g c c t g t c c c g t t c g g a t c t c c

3420 QPNTALLSLVLMAGTFFIAF LWICGQQGQHQHCAGEKDGE a c a g c c a a a t a c a g c c c t g c t g t c c c t g g t g c t g a t g g c a g g c a c c t t c t t t a t c g c c t t t g t c g g t t t a t g t c g g g a c g a c a g g g a c c a c g a c t a c c g t c c g t g g a a g a a a t a g c g g a a

BamHIBspEI* 3480 FLRKFKNSRFFPGRIRRVIG KQPLELVRAEKWAPDPSHDA c t t t c t g a g g a a g t t c a a g a a c t c t c g c t t c t t t c c a g g c c g g a t c c g g a g a g t g a t c g g g a a a g a c t c c t t c a a g t t c t t g a g a g c g a a g a a a g g t c c g g c c t a g g c c t c t c a c t a g c c

XcmIBtgZI 3540 DFGVPIAILIMVLVDYSIED VKAHGDGDQDHHQHIVGDLV c g a c t t t g g c g t g c c c a t c g c c a t c c t g a t c a t g g t g c t g g t g g a t t a c t c c a t c g a g g a g c t g a a a c c g c a c g g g t a g c g g t a g g a c t a g t a c c a c g a c c a c c t a a t g a g g t a g c t c c t

3600 TYTQKLSVPSGFSVTAPEKR GICLLQGHWRAEAHCCWLLP *VCFSDTGDPKLTVAGSFL c a c c t a t a c a c a g a a g c t g t c c g t g c c a t c t g g c t t c a g c g t g a c a g c a c c a g a g a a g a g g t g g a t a t g t g t c t t c g a c a g g c a c g g t a g a c c g a a g t c g c a c t g t c g t g g t c t c t t c t c

3660 GWVINPLGEKSPFPVWMMVA SPDDVRQALLRRKWHPHHHG PHTMLGRPSFDGKGTHIITA g g g a t g g g t c a t c a a c c c t c t g g g c g a g a a g t c t c c t t t t c c a g t g t g g a t g a t g g t g g c c c c t a c c c a g t a g t t g g g a g a c c c g c t c t t c a g a g g a a a a g g t c a c a c c t a c t a c c a c c g

3720 SLLPAILVFILIFMETQITT AQQWGDQHKDQDEHLGLDGC LRSGAMRTNIRIKMSVWIVV c a g c c t g c t g c c a g c c a t c c t g g t g t t t a t c c t g a t c t t c a t g g a g a c c c a g a t c a c c a c g t c g g a c g a c g g t c g g t a g g a c c a c a a a t a g g a c t a g a a g t a c c t c t g g g t c t a g t g g t g

SphI 3780 LIISKKERMLQKGSGFHLDL QDDGLLLAHQLLARAEVQIQ SIMELFSRMSCFPEPKWRSR a c t g a t c a t c t c c a a g a a g g a g c g c a t g c t g c a g a a g g g c t c t g g c t t c c a c c t g g a t c t t g a c t a g t a g a g g t t c t t c c t c g c g t a c g a c g t c t t c c c g a g a c c g a a g g t g g a c c t a g a

3840 LLIVAMGGICALFGLPWLAA QQDHGHAADTGQKSQWPQCC SSITAMPPMQARNPSGHSAA g c t g c t g a t c g t g g c c a t g g g c g g c a t c t g t g c c c t g t t t g g a c t g c c a t g g c t g g c a g c c g a c g a c t a g c a c c g g t a c c c g c c g t a g a c a c g g g a c a a a c c t g a c g g t a c c g a c c g t c g

RsrII 3900 ATVRSVTHANALTVMSKAVA CGHPGHCVCVCQCHHRLGHG AVTRDTVCAFASVTIDLATA a g c a a c c g t g c g g t c c g t g a c a c a c g c a a a c g c a c t g a c a g t g a t g t c t a a g g c c g t g g c t c g t t g g c a c g c c a g g c a c t g t g t g c g t t t g c g t g a c t g t c a c t a c a g a t t c c g g c a c c g

3960 PGDKPKIQEVKEQRVTGLLV GAILRLDLLHLLLAHGSQQH GPSLGLIWSTFSCRTVPSST c c c c g g c g a t a a g c c t a a g a t c c a g g a g g t g a a g g a g c a g c g c g t g a c c g g a c t g c t g g t g g g g c c g c t a t t c g g a t t c t a g g t c c t c c a c t t c c t c g t c g c g c a c t g g c c t g a c g a c c a

BbvCI 4020 ALLVGLSIVIGDLLRQIPLA GQQHSQGDHDAVQQPLDWQG ARSTPSDMTIPSRSLCIGSA g g c c c t g c t g g t g g g a c t g t c c a t c g t g a t c g g c g a c c t g c t g a g g c a g a t c c c a c t g g c c c g g g a c g a c c a c c c t g a c a g g t a g c a c t a g c c g c t g g a c g a c t c c g t c t a g g g t g a c c g

4080 VLFGIFLYMGVTSLNGIQFY HQEADKQVHAHGRQIADLEI TSNPMKRYMPTVERFPM c g t g c t g t t c g g c a t c t t t c t g t a c a t g g g c g t g a c c t c t c t g a a t g g c a t c c a g t t c t a g c a c g a c a a g c c g t a g a a a g a c a t g t a c c c g c a c t g g a g a g a c t t a c c g t a g g t c a a g a t

4140 ERLHLLLMPPKHHPDVTYVK LPQVQQQHGRLVVGVHGVHL t g a g c g g c t g c a c c t g c t g c t g a t g c c c c c t a a g c a c c a c c c c g a c g t g a c c t a c g t g a a a c t c g c c g a c g t g g a c g a c g a c t a c g g g g g a t t c g t g g t g g g g c t g c a c t g g a t g c a c t t

FspAI 4200 KVRTLRMHLFTALQLLCLAL LHACQPHVQKGGQLQQAQGQ g a a g g t g c g c a c a c t g c g g a t g c a c c t g t t t a c c g c c c t g c a g c t g c t g t g c c t g g c c c t c t t c c a c g c g t g t g a c g c c t a c g t g g a c a a a t g g c g g g a c g t c g a c g a c a c g g a c c g g g a

4260 LWAVMSTAASLAFPFILILT QPGHHGCGGRQGERKDQDQG g c t g t g g g c c g t g a t g t c c a c a g c c g c c t c t c t g g c c t t c c c t t t t a t c c t g a t c c t g a c c g a c a c c c g g c a c t a c a g g t g t c g g c g g a g a g a c c g g a a g g g a a a a t a g g a c t a g g a c t g

4320 VPLRMVVLTRIFTDREMKCL HWQAHHHQGSDECIPLHLTQ c g t g c c a c t g c g c a t g g t g g t g c t g a c c a g a a t c t t c a c a g a t a g g g a g a t g a a g t g t c t g c a c g g t g a c g c g t a c c a c c a c g a c t g g t c t t a g a a g t g t c t a t c c c t c t a c t t c a c a g a

4380 DANEAEPVFDEREGVDEYNE VGVLGLGHKILPLAHVLIIL g g a c g c c a a c g a g g c c g a g c c c g t g t t t g a t g a g c g g g a g g g c g t g g a c g a g t a t a a t g a c c t g c g g t t g c t c c g g c t c g g g c a c a a a c t a c t c g c c c t c c c g c a c c t g c t c a t a t t a c t

BstXI 4440 attL2 MPMPVDPAFLYKVGIIRKHC HGHRHVWSEQVFNANYSLM g a t g c c c a t g c c t g t g g a c c c a g c t t t c t t g t a c a a a g t t g g c a t t a t a a g a a a g c a t t g c t a c g g g t a c g g a c a c c t g g g t c g a a a g a a c a t g t t t c a a c c g t a a t a t t c t t t c g t a a c

4500 attL2 LSICCNEQVTISQNKIIICH c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c c a g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g g t

EcoRV 4560 T7 promoter M13 rev G G G A T A T C A C T C A G C A T A A T T7 C A G T A T C G A C A A A G G A C M13 Reverse PADIPYSESYYMVIAVSWQL t c c a g c t g a t a t c c c c t a t a g t g a g t c g t a t t a c a t g g t c a t a g c t g t t t c c t g g c a g c t a g g t c g a c t a t a g g g g a t a t c a c t c a g c a t a a t g t a c c a g t a t c g a c a a a g g a c c g t c g a

4620 WPVSQNL* c t g g c c c g t g t c t c a a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g g a c c g g g c a c a g a g t t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c

4680 1 5 MSHIQ KanR G T T C C C C A C A A T A C T C G G T A pENTR-R MSHIQ a a c a a t a a a a c t g t c t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t a t g a g c c a t a t t c a t t g t t a t t t t g a c a g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a t a c t c g g t a t a a g t

4740 10 15 20 25 RETSRPRLNSNMDADLYGYK KanR C C A T A T T Kan-R RETSRPRLNSNMDADLYGYK a c g g g a a a c g t c g a g g c c g c g a t t a a a t t c c a a c a t g g a t g c t g a t t t a t a t g g g t a t a a t g c c c t t t g c a g c t c c g g c g c t a a t t t a a g g t t g t a c c t a c g a c t a a a t a t a c c c a t a t t

NruI 4800 30 35 40 45 WARDNVGQSGATIYRLYGKP KanR T A C C C G A G C G C T A Kan-R WARDNVGQSGATIYRLYGKP a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t c t a t c g c t t g t a t g g g a a g c c t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a g a t a g c g a a c a t a c c c t t c g g

4860 50 55 60 65 DAPELFLKHGKGSVANDVTD KanR DAPELFLKHGKGSVANDVTD c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g c g t t g c c a a t g a t g t t a c a g a g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c g c a a c g g t t a c t a c a a t g t c t

4920 70 75 80 85 EMVRLNWLTEFMPLPTIKHF KanR EMVRLNWLTEFMPLPTIKHF t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c t c t t c c g a c c a t c a a g c a t t t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g a g a a g g c t g g t a g t t c g t a a a

4980 90 95 100 105 IRTPDDAWLLTTAIPGKTAF KanR IRTPDDAWLLTTAIPGKTAF t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c g a t c c c c g g a a a a a c a g c a t t a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g c t a g g g g c c t t t t t g t c g t a a

SspI 5040 110 115 120 125 QVLEEYPDSGENIVDALAVF KanR QVLEEYPDSGENIVDALAVF c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t t g t t g a t g c g c t g g c a g t g t t g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a a c a a c t a c g c g a c c g t c a c a a

AsiSIPvuI 5100 130 135 140 145 LRRLHSIPVCNCPFNSDRVF KanR LRRLHSIPVCNCPFNSDRVF c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c t t t t a a c a g c g a t c g c g t a t t g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g a a a a t t g t c g c t a g c g c a t a a

BsmBIEsp3I 5160 150 155 160 165 RLAQAQSRMNNGLVDASDFD KanR RLAQAQSRMNNGLVDASDFD t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t g g t t g a t g c g a g t g a t t t t g a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a c c a a c t a c g c t c a c t a a a a c t

5220 170 175 180 185 DERNGWPVEQVWKEMHKLLP KanR DERNGWPVEQVWKEMHKLLP t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a a g a a a t g c a t a a a c t t t t g c c a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t t c t t t a c g t a t t t g a a a a c g g

5280 190 195 200 205 FSPDSVVTHGDFSLDNLIFD KanR FSPDSVVTHGDFSLDNLIFD a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c a c t t g a t a a c c t t a t t t t t g a t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g t g a a c t a t t g g a a t a a a a a c t

5340 210 215 220 225 EGKLIGCIDVGRVGIADRYQ KanR EGKLIGCIDVGRVGIADRYQ c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t c g g a a t c g c a g a c c g a t a c c a g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a g c c t t a g c g t c t g g c t a t g g t

5400 230 235 240 245 DLAILWNCLGEFSPSLQKRL KanR DLAILWNCLGEFSPSLQKRL g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c t c c t t c a t t a c a g a a a c g g c t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g a g g a a g t a a t g t c t t t g c c g a

5460 250 255 260 265 FQKYGIDNPDMNKLQFHLML KanR FQKYGIDNPDMNKLQFHLML t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a t t g c a g t t t c a t t t g a t g c t a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t t a a c g t c a a a g t a a a c t a c g a

5520 270 DEFF* KanR DEFF* c g a t g a g t t t t t c t a a t c a g a a t t g g t t a a t t g g t t g t a a c a c t g g c a g a g c a t t a c g c t g c t a c t c a a a a a g a t t a g t c t t a a c c a a t t a a c c a a c a t t g t g a c c g t c t c g t a a t g c g a

5580 g a c t t g a c g g g a c g g c g c a a g c t c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c t g a a c t g c c c t g c c g c g t t c g a g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a

5640 ori c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a

5700 ori g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g

5760 ori g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g

5820 ori a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g

5880 ori g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g

5940 ori g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c

ApaLI 6000 ori a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t

6060 ori c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t

BciVI 6120 ori G G G A A A C G C pBR322ori-F t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g

6180 ori C T G G T A T C T T T pBR322ori-F c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c

6240 ori a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a

PciI 3ʹ 5ʹ 6273 c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.