Addgene pLJM1-FLAG-GFP-OSBP - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLJM1-FLAG-GFP-OSBP

Map View

hU6-F (10,386 .. 10,406) BspDI * - ClaI * (10,346) KflI (10,257) BbvCI (9747) MfeI (9512) NruI * (9160) SphI (8644) T3 promoter T3 (8506 .. 8526) M13 rev M13 Reverse (8471 .. 8487) lac operator M13/pUC Reverse (8452 .. 8474) lac promoter CAP binding site L4440 (8239 .. 8256) pBR322ori-F (7986 .. 8005) ScaI (6772) Amp-R (6684 .. 6703) F1ori-F (6177 .. 6198) F1ori-R (5967 .. 5986) M13/pUC Forward (5731 .. 5753) M13 Forward (5722 .. 5739) M13 fwd T7 (5693 .. 5712) T7 promoter AvrII (5672) SfiI (5625) EBV-rev (5468 .. 5487) SnaBI (110) CMV-F (289 .. 309) NheI (361) BmtI (365) AgeI (370) EGFP-N (463 .. 484) EXFP-R (724 .. 743) EGFP-C (1071 .. 1092) SalI (1167) PshAI (1321) TspMI - XmaI (1344) SmaI (1346) PmlI (1841) BclI * (1988) EcoRI (2574) XbaI (2580) BstXI (2878) HpaI (3521) SpeI (4069) AscI (4158) hPGK-F (4242 .. 4260) BamHI (4326) Puro-R (4345 .. 4364) PflFI - Tth111I (4387) BsiWI (4401) RsrII (4461) BstEII (4479) Puro-F (4841 .. 4861) NsiI (4998) SV40pA-R (5414 .. 5433) pLJM1-FLAG-GFP-OSBP 10,560 bp

Sequence View

5ʹ 3ʹ 60 a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 120 a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

180 CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

240 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

300 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

360 CMV promoter G T A G G C G T G CMV-F g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g

AgeIBmtINheI 420 1 5 DYKDDDDK (in frame with FLAG) GGGGS FLAG MDYKDDDDKGGGGS g c t a g c g c t a c c g g t g c c a t g g a c t a c a a g g a t g a c g a t g a c a a g g g c g g a g g t g g a t c t c g a t c g c g a t g g c c a c g g t a c c t g a t g t t c c t a c t g c t a c t g t t c c c g c c t c c a c c t a g a

480 1a V 5 10 15 20 SKGEELFTGVVPILVELDG EGFP 1a V 5 10 15 20 SKGEELFTGVVPILVELDG EGFP G A C C A G C T C G A C C T G C C G EGFP-N VSKGEELFTGVVPILVELDG g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g

540 25 30 35 40 DVNGHKFSVSGEGEGDATYG EGFP 25 30 35 40 DVNGHKFSVSGEGEGDATYG EGFP C T G C EGFP-N DVNGHKFSVSGEGEGDATYG g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g

600 45 50 55 60 KLTLKFICTTGKLPVPWPTL EGFP 45 50 55 60 KLTLKFICTTGKLPVPWPTL EGFP KLTLKFICTTGKLPVPWPTL a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g

660 65 70 75 80 VTTLTYGVQCFSRYPDHMKQ EGFP 65 70 75 80 VTTLTYGVQCFSRYPDHMKQ EGFP VTTLTYGVQCFSRYPDHMKQ g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c

720 85 90 95 100 HDFFKSAMPEGYVQERTIFF EGFP 85 90 95 100 HDFFKSAMPEGYVQERTIFF EGFP HDFFKSAMPEGYVQERTIFF c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g

780 105 110 115 120 KDDGNYKTRAEVKFEGDTLV EGFP 105 110 115 120 KDDGNYKTRAEVKFEGDTLV EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R KDDGNYKTRAEVKFEGDTLV a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c

840 125 130 135 140 NRIELKGIDFKEDGNILGHK EGFP 125 130 135 140 NRIELKGIDFKEDGNILGHK EGFP NRIELKGIDFKEDGNILGHK a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c

900 145 150 155 160 LEYNYNSHNVYIMADKQKNG EGFP 145 150 155 160 LEYNYNSHNVYIMADKQKNG EGFP LEYNYNSHNVYIMADKQKNG c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g

960 165 170 175 180 IKVNFKIRHNIEDGSVQLAD EGFP 165 170 175 180 IKVNFKIRHNIEDGSVQLAD EGFP IKVNFKIRHNIEDGSVQLAD a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g

1020 185 190 195 200 HYQQNTPIGDGPVLLPDNHY EGFP 185 190 195 200 HYQQNTPIGDGPVLLPDNHY EGFP HYQQNTPIGDGPVLLPDNHY c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g

1080 205 210 215 220 LSTQSALSKDPNEKRDHMVL EGFP 205 210 215 220 LSTQSALSKDPNEKRDHMVL EGFP C A T G G T C C T G EGFP-C LSTQSALSKDPNEKRDHMVL c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c

1140 225 230 235 LEFVTAAGITLGMDELYK GG EGFP 225 230 235 LEFVTAAGITLGMDELYK GG EGFP C T G G A G T T C G T G EGFP-C LEFVTAAGITLGMDELYKGG c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g a g g c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c t c c g

SalI 1200 (in frame with EGFP) GGSGGGGSKSTMAATELRGV (in frame with EGFP) GGSGGGGSKSTMAATELRGV GGSGGGGSKSTMAATELRGV g g t g g a t c g g g c g g g g g t g g a t c a a a g t c g a c g a t g g c t g c t a c g g a g c t g a g a g g a g t g c c a c c t a g c c c g c c c c c a c c t a g t t t c a g c t g c t a c c g a c g a t g c c t c g a c t c t c c t c a c

1260 (in frame with EGFP) VGPGPAAIAALGGGGAGPPV (in frame with EGFP) VGPGPAAIAALGGGGAGPPV VGPGPAAIAALGGGGAGPPV g t g g g t c c a g g a c c a g c a g c t a t t g c a g c a c t t g g a g g t g g a g g a g c c g g t c c t c c a g t t c a c c c a g g t c c t g g t c g t c g a t a a c g t c g t g a a c c t c c a c c t c c t c g g c c a g g a g g t c a a

1320 (in frame with EGFP) VGGGGGRGDAGPGSGAASGT (in frame with EGFP) VGGGGGRGDAGPGSGAASGT VGGGGGRGDAGPGSGAASGT g t a g g a g g a g g t g g a g g a c g c g g a g a t g c g g g a c c a g g c t c c g g t g c c g c g t c a g g g a c g c a t c c t c c t c c a c c t c c t g c g c c t c t a c g c c c t g g t c c g a g g c c a c g g c g c a g t c c c t g c

TspMIXmaISmaIPshAI 1380 (in frame with EGFP) VVAAAAGGPGPGAGGVAAAG (in frame with EGFP) VVAAAAGGPGPGAGGVAAAG VVAAAAGGPGPGAGGVAAAG g t g g t c g c a g c a g c a g c t g g a g g c c c g g g a c c t g g t g c a g g t g g a g t t g c a g c t g c a g g t c a c c a g c g t c g t c g t c g a c c t c c g g g c c c t g g a c c a c g t c c a c c t c a a c g t c g a c g t c c a

1440 (in frame with EGFP) PAPAPPTGGSGGSGAGGSGS (in frame with EGFP) PAPAPPTGGSGGSGAGGSGS PAPAPPTGGSGGSGAGGSGS c c t g c a c c t g c t c c g c c t a c t g g a g g t t c a g g a g g t t c a g g a g c t g g a g g t t c a g g a t c g g g a c g t g g a c g a g g c g g a t g a c c t c c a a g t c c t c c a a g t c c t c g a c c t c c a a g t c c t a g c

1500 (in frame with EGFP) AREGWLFKWTNYIKGYQRRW (in frame with EGFP) AREGWLFKWTNYIKGYQRRW AREGWLFKWTNYIKGYQRRW g c t c g a g a g g g c t g g c t c t t c a a a t g g a c c a a t t a t a t c a a a g g c t a c c a g c g g c g a t g g c g a g c t c t c c c g a c c g a g a a g t t t a c c t g g t t a a t a t a g t t t c c g a t g g t c g c c g c t a c c

1560 (in frame with EGFP) FVLSNGLLSYYRSKAEMRHT (in frame with EGFP) FVLSNGLLSYYRSKAEMRHT FVLSNGLLSYYRSKAEMRHT t t c g t g c t g a g c a a c g g g c t c c t g a g c t a c t a c a g a t c a a a g g c a g a a a t g a g a c a t a c c a a g c a c g a c t c g t t g c c c g a g g a c t c g a t g a t g t c t a g t t t c c g t c t t t a c t c t g t a t g g

1620 (in frame with EGFP) CRGTINLATANITVEDSCNF (in frame with EGFP) CRGTINLATANITVEDSCNF CRGTINLATANITVEDSCNF t g c c g t g g t a c c a t c a a c c t c g c c a c a g c c a a c a t c a c c g t g g a g g a c t c c t g c a a c t t c a c g g c a c c a t g g t a g t t g g a g c g g t g t c g g t t g t a g t g g c a c c t c c t g a g g a c g t t g a a g

1680 (in frame with EGFP) IISNGGAQTYHLKASSEVER (in frame with EGFP) IISNGGAQTYHLKASSEVER IISNGGAQTYHLKASSEVER a t c a t t t c c a a t g g g g g t g c t c a g a c c t a c c a t c t g a a a g c t a g t t c a g a a g t t g a g c g g t a g t a a a g g t t a c c c c c a c g a g t c t g g a t g g t a g a c t t t c g a t c a a g t c t t c a a c t c g c c

1740 (in frame with EGFP) QRWVTALELAKAKAVKMLAE (in frame with EGFP) QRWVTALELAKAKAVKMLAE QRWVTALELAKAKAVKMLAE c a g c g c t g g g t g a c g g c c c t g g a a c t g g c c a a g g c c a a a g c t g t g a a g a t g c t g g c a g a g g t c g c g a c c c a c t g c c g g g a c c t t g a c c g g t t c c g g t t t c g a c a c t t c t a c g a c c g t c t c

1800 (in frame with EGFP) SDESGDEESVSQTDKTELQN (in frame with EGFP) SDESGDEESVSQTDKTELQN SDESGDEESVSQTDKTELQN t c a g a t g a a t c a g g a g a t g a a g a g t c t g t c t c a c a a a c t g a c a a g a c t g a g c t g c a g a a t a g t c t a c t t a g t c c t c t a c t t c t c a g a c a g a g t g t t t g a c t g t t c t g a c t c g a c g t c t t a

PmlI 1860 (in frame with EGFP) TLRTLSSKVEDLSTCNDLIA (in frame with EGFP) TLRTLSSKVEDLSTCNDLIA TLRTLSSKVEDLSTCNDLIA a c c c t t c g g a c c c t c t c t a g c a a a g t a g a g g a c t t g a g c a c g t g c a a t g a c t t g a t a g c t t g g g a a g c c t g g g a g a g a t c g t t t c a t c t c c t g a a c t c g t g c a c g t t a c t g a a c t a t c g a

1920 (in frame with EGFP) KHGTALQRSLSELESLKLPA (in frame with EGFP) KHGTALQRSLSELESLKLPA KHGTALQRSLSELESLKLPA a a g c a t g g c a c a g c t c t g c a g c g t t c t c t c a g t g a g c t g g a g t c c c t g a a g t t g c c t g c t t t c g t a c c g t g t c g a g a c g t c g c a a g a g a g t c a c t c g a c c t c a g g g a c t t c a a c g g a c g a

1980 (in frame with EGFP) ESNEKIKQVNERATLFRITS (in frame with EGFP) ESNEKIKQVNERATLFRITS ESNEKIKQVNERATLFRITS g a g a g c a a t g a a a a g a t c a a a c a g g t c a a c g a a c g a g c c a c a c t c t t t a g g a t a a c a t c c c t c t c g t t a c t t t t c t a g t t t g t c c a g t t g c t t g c t c g g t g t g a g a a a t c c t a t t g t a g g

BclI* 2040 (in frame with EGFP) NAMINACRDFLMLAQTHSKK (in frame with EGFP) NAMINACRDFLMLAQTHSKK NAMINACRDFLMLAQTHSKK a a t g c c a t g a t c a a c g c c t g c a g a g a t t t c c t c a t g t t a g c c c a g a c c c a t a g t a a a a a a t t a c g g t a c t a g t t g c g g a c g t c t c t a a a g g a g t a c a a t c g g g t c t g g g t a t c a t t t t t t

2100 (in frame with EGFP) WQKSLQYERDQRIRLEETLE (in frame with EGFP) WQKSLQYERDQRIRLEETLE WQKSLQYERDQRIRLEETLE t g g c a a a a g t c a c t a c a g t a t g a a a g a g a c c a g c g t a t c c g a c t g g a a g a a a c c c t c g a g a c c g t t t t c a g t g a t g t c a t a c t t t c t c t g g t c g c a t a g g c t g a c c t t c t t t g g g a g c t c

2160 (in frame with EGFP) QLAKQHNHLERAFRGATVLP (in frame with EGFP) QLAKQHNHLERAFRGATVLP QLAKQHNHLERAFRGATVLP c a g c t g g c g a a g c a g c a t a a t c a c c t g g a g a g g g c c t t c c g a g g a g c c a c g g t g c t g c c g g t c g a c c g c t t c g t c g t a t t a g t g g a c c t c t c c c g g a a g g c t c c t c g g t g c c a c g a c g g c

2220 (in frame with EGFP) ANTPGNVGSGKDQCCSGKGD (in frame with EGFP) ANTPGNVGSGKDQCCSGKGD ANTPGNVGSGKDQCCSGKGD g c a a a c a c t c c t g g c a a t g t g g g t t c t g g t a a a g a t c a g t g c t g c t c t g g c a a a g g g g a c c g t t t g t g a g g a c c g t t a c a c c c a a g a c c a t t t c t a g t c a c g a c g a g a c c g t t t c c c c t g

2280 (in frame with EGFP) MSDEDDENEFFDAPEIITMP (in frame with EGFP) MSDEDDENEFFDAPEIITMP MSDEDDENEFFDAPEIITMP a t g a g c g a t g a a g a t g a t g a g a a t g a a t t t t t t g a t g c a c c t g a g a t c a t c a c c a t g c c t t a c t c g c t a c t t c t a c t a c t c t t a c t t a a a a a a c t a c g t g g a c t c t a g t a g t g g t a c g g a

2340 (in frame with EGFP) ENLGHKRTGSNISGASSDIS (in frame with EGFP) ENLGHKRTGSNISGASSDIS ENLGHKRTGSNISGASSDIS g a a a a t t t g g g c c a c a a a c g t a c t g g c a g c a a t a t c a g t g g a g c c a g c a g t g a c a t c a g c c t t t t a a a c c c g g t g t t t g c a t g a c c g t c g t t a t a g t c a c c t c g g t c g t c a c t g t a g t c g

2400 (in frame with EGFP) LDEQYKHQLEETKKEKRTRI (in frame with EGFP) LDEQYKHQLEETKKEKRTRI LDEQYKHQLEETKKEKRTRI c t t g a t g a a c a g t a c a a g c a t c a g c t g g a g g a g a c c a a a a a g g a a a a g a g a a c c a g a a t a g a a c t a c t t g t c a t g t t c g t a g t c g a c c t c c t c t g g t t t t t c c t t t t c t c t t g g t c t t a t

2460 (in frame with EGFP) PYKPNYSLNLWSIMKNCIGK (in frame with EGFP) PYKPNYSLNLWSIMKNCIGK PYKPNYSLNLWSIMKNCIGK c c a t a c a a g c c a a a c t a t a g c c t c a a t t t a t g g a g c a t c a t g a a g a a c t g c a t t g g a a a a g g t a t g t t c g g t t t g a t a t c g g a g t t a a a t a c c t c g t a g t a c t t c t t g a c g t a a c c t t t t

2520 (in frame with EGFP) ELSKIPMPVNFNEPLSMLQR (in frame with EGFP) ELSKIPMPVNFNEPLSMLQR ELSKIPMPVNFNEPLSMLQR g a a c t c t c t a a g a t c c c c a t g c c g g t a a a c t t t a a t g a g c c c t t g t c c a t g c t t c a g c g c c t t g a g a g a t t c t a g g g g t a c g g c c a t t t g a a a t t a c t c g g g a a c a g g t a c g a a g t c g c g

EcoRI 2580 (in frame with EGFP) LTEDLEYHELLDRAAKCENS (in frame with EGFP) LTEDLEYHELLDRAAKCENS LTEDLEYHELLDRAAKCENS c t t a c t g a a g a t c t g g a a t a c c a t g a g c t g t t a g a c c g a g c t g c a a a a t g t g a g a a t t c t g a a t g a c t t c t a g a c c t t a t g g t a c t c g a c a a t c t g g c t c g a c g t t t t a c a c t c t t a a g a

XbaI 2640 (in frame with EGFP) LEQLCYVAAFTVSSYSTTVF (in frame with EGFP) LEQLCYVAAFTVSSYSTTVF LEQLCYVAAFTVSSYSTTVF c t a g a a c a g c t c t g t t a t g t t g c a g c t t t c a c c g t g t c c t c c t a c t c c a c t a c t g t c t t c g a t c t t g t c g a g a c a a t a c a a c g t c g a a a g t g g c a c a g g a g g a t g a g g t g a t g a c a g a a g

2700 (in frame with EGFP) RTSKPFNPLLGETFELDRLE (in frame with EGFP) RTSKPFNPLLGETFELDRLE RTSKPFNPLLGETFELDRLE c g c a c c a g t a a g c c a t t c a a c c c a c t g c t t g g g g a g a c c t t t g a g c t g g a c c g a t t a g a g g c g t g g t c a t t c g g t a a g t t g g g t g a c g a a c c c c t c t g g a a a c t c g a c c t g g c t a a t c t c

2760 (in frame with EGFP) ENGYRSLCEQVSHHPPAAAH (in frame with EGFP) ENGYRSLCEQVSHHPPAAAH ENGYRSLCEQVSHHPPAAAH g a g a a t g g g t a c c g a t c c c t c t g t g a a c a g g t g a g t c a t c a t c c c c c t g c t g c t g c g c a c c t c t t a c c c a t g g c t a g g g a g a c a c t t g t c c a c t c a g t a g t a g g g g g a c g a c g a c g c g t g

2820 (in frame with EGFP) HAESKNGWTLRQEIKITSKF (in frame with EGFP) HAESKNGWTLRQEIKITSKF HAESKNGWTLRQEIKITSKF c a t g c t g a g t c c a a a a a t g g c t g g a c a t t g c g t c a g g a a a t c a a a a t c a c c a g c a a g t t t g t a c g a c t c a g g t t t t t a c c g a c c t g t a a c g c a g t c c t t t a g t t t t a g t g g t c g t t c a a a

BstXI 2880 (in frame with EGFP) RGKYLSIMPLGTIHCIFHAT (in frame with EGFP) RGKYLSIMPLGTIHCIFHAT RGKYLSIMPLGTIHCIFHAT c g a g g c a a a t a c c t c t c c a t t a t g c c c c t c g g t a c c a t t c a t t g t a t t t t c c a t g c a a c t g c t c c g t t t a t g g a g a g g t a a t a c g g g g a g c c a t g g t a a g t a a c a t a a a a g g t a c g t t g a

2940 (in frame with EGFP) GHHYTWKKVTTTVHNIIVGK (in frame with EGFP) GHHYTWKKVTTTVHNIIVGK GHHYTWKKVTTTVHNIIVGK g g g c a c c a c t a c a c t t g g a a g a a a g t t a c c a c a a c t g t a c a c a a c a t t a t t g t g g g c a a g c c c g t g g t g a t g t g a a c c t t c t t t c a a t g g t g t t g a c a t g t g t t g t a a t a a c a c c c g t t c

3000 (in frame with EGFP) LWIDQSGEIDIVNHKTGDKC (in frame with EGFP) LWIDQSGEIDIVNHKTGDKC LWIDQSGEIDIVNHKTGDKC t t g t g g a t a g a t c a g t c t g g c g a a a t t g a t a t t g t g a a t c a c a a g a c a g g a g a c a a g t g t a a c a c c t a t c t a g t c a g a c c g c t t t a a c t a t a a c a c t t a g t g t t c t g t c c t c t g t t c a c a

3060 (in frame with EGFP) NLKFVPYSYFSRDVARKVTG (in frame with EGFP) NLKFVPYSYFSRDVARKVTG NLKFVPYSYFSRDVARKVTG a a t c t t a a a t t t g t t c c t t a t a g c t a c t t c t c t c g g g a t g t a g c a a g a a a g g t g a c g g g g t t a g a a t t t a a a c a a g g a a t a t c g a t g a a g a g a g c c c t a c a t c g t t c t t t c c a c t g c c c c

3120 (in frame with EGFP) EVTDPSGKVHFALLGTWDEK (in frame with EGFP) EVTDPSGKVHFALLGTWDEK EVTDPSGKVHFALLGTWDEK g a a g t g a c a g a t c c a t c a g g a a a a g t c c a c t t t g c t c t t c t g g g g a c g t g g g a t g a g a a a c t t c a c t g t c t a g g t a g t c c t t t t c a g g t g a a a c g a g a a g a c c c c t g c a c c c t a c t c t t t

3180 (in frame with EGFP) MECFKVQPVIGENGGDARQR (in frame with EGFP) MECFKVQPVIGENGGDARQR MECFKVQPVIGENGGDARQR a t g g a a t g t t t c a a a g t a c a g c c a g t c a t t g g g g a a a a t g g g g g t g a t g c t c g a c a g a g a t a c c t t a c a a a g t t t c a t g t c g g t c a g t a a c c c c t t t t a c c c c c a c t a c g a g c t g t c t c t

3240 (in frame with EGFP) GHEAEESRVMLWKRNPLPKN (in frame with EGFP) GHEAEESRVMLWKRNPLPKN GHEAEESRVMLWKRNPLPKN g g c c a t g a a g c a g a g g a a a g c a g g g t c a t g c t g t g g a a a a g g a a t c c t t t a c c g a a g a a t c c g g t a c t t c g t c t c c t t t c g t c c c a g t a c g a c a c c t t t t c c t t a g g a a a t g g c t t c t t a

3300 (in frame with EGFP) AENMYYFSELALTLNAWESG (in frame with EGFP) AENMYYFSELALTLNAWESG AENMYYFSELALTLNAWESG g c a g a a a a c a t g t a c t a c t t c t c a g a g c t t g c t c t g a c t c t c a a t g c t t g g g a a a g t g g c c g t c t t t t g t a c a t g a t g a a g a g t c t c g a a c g a g a c t g a g a g t t a c g a a c c c t t t c a c c g

3360 (in frame with EGFP) TAPTDSRLRPDQRLMENGRW (in frame with EGFP) TAPTDSRLRPDQRLMENGRW TAPTDSRLRPDQRLMENGRW a c t g c c c c c a c a g a c a g c c g g t t a c g a c c t g a c c a g a g a c t g a t g g a a a a t g g a c g c t g g t g a c g g g g g t g t c t g t c g g c c a a t g c t g g a c t g g t c t c t g a c t a c c t t t t a c c t g c g a c c

3420 (in frame with EGFP) DEANAEKQRLEEKQRLSRKK (in frame with EGFP) DEANAEKQRLEEKQRLSRKK DEANAEKQRLEEKQRLSRKK g a t g a a g c a a a t g c g g a g a a g c a g c g c c t g g a g g a a a a a c a a a g a c t t t c c a g a a a g a a g c t a c t t c g t t t a c g c c t c t t c g t c g c g g a c c t c c t t t t t g t t t c t g a a a g g t c t t t c t t c

3480 (in frame with EGFP) REAEAMKATEDGTPYDPYKA (in frame with EGFP) REAEAMKATEDGTPYDPYKA REAEAMKATEDGTPYDPYKA a g a g a a g c g g a a g c t a t g a a a g c c a c a g a g g a t g g c a c a c c a t a t g a t c c c t a t a a g g c a t c t c t t c g c c t t c g a t a c t t t c g g t g t c t c c t a c c g t g t g g t a t a c t a g g g a t a t t c c g t

HpaI 3540 (in frame with EGFP) LWFERKKDPVTKELTHIYRG (in frame with EGFP) LWFERKKDPVTKELTHIYRG LWFERKKDPVTKELTHIYRG c t g t g g t t t g a g c g g a a g a a g g a c c c t g t t a c c a a g g a g t t a a c c c a t a t t t a t a g g g g a g a c a c c a a a c t c g c c t t c t t c c t g g g a c a a t g g t t c c t c a a t t g g g t a t a a a t a t c c c c t

3600 (in frame with EGFP) EYWECKEKQDWSSCPDIF* (in frame with EGFP) EYWECKEKQDWSSCPDIF* EYWECKEKQDWSSCPDIF* MPGHFLS g a a t a c t g g g a g t g t a a a g a a a a a c a g g a c t g g a g c t c a t g c c c g g a c a t t t t c t g a g c g c t t a t g a c c c t c a c a t t t c t t t t t g t c c t g a c c t c g a g t a c g g g c c t g t a a a a g a c t c g c

3660 cPPT/CTS ADQFSTSRQMAVFIHNFKRK c g g a t c a a t t c t c g a c c t c g a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g c c t a g t t a a g a g c t g g a g c t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c

3720 cPPT/CTS GGIGGYSAGERIVDIIATDI g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g

3780 cPPT/CTS QTKELQKQITKIQNFRVYYR *LFLNCFNLIKPNIVP a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c

3840 hPGK promoter DSRDPLWPRLEGVGVAPFPR VASIWKPRPELPNPNRRKWP a c a g c a g a g a t c c a c t t t g g c c g c g g c t c g a g g g g g t t g g g g t t g c g c c t t t t c c a a g g c t g t c g t c t c t a g g t g a a a c c g g c g c c g a g c t c c c c c a a c c c c a a c g c g g a a a a g g t t c c g

3900 hPGK promoter QPWVCAGTRLLWAWFRETQR LGPNACPRPQEPRPEPFRLP a g c c c t g g g t t t g c g c a g g g a c g c g g c t g c t c t g g g c g t g g t t c c g g g a a a c g c a g c g g c t c g g g a c c c a a a c g c g t c c c t g c g c c g a c g a g a c c c g c a c c a a g g c c c t t t g c g t c g c c g

3960 hPGK promoter RRPWVSHILHVRSQRHPDLR ASGPDRVNKVDTRLTVRIKA g c c g a c c c t g g g t c t c g c a c a t t c t t c a c g t c c g t t c g c a g c g t c a c c c g g a t c t t c g c c c g g c t g g g a c c c a g a g c g t g t a a g a a g t g c a g g c a a g c g t c g c a g t g g g c c t a g a a g c g g

4020 hPGK promoter RYPCGPPGDASCSAPKSGRF AVRTPGGAVSGAGGRLRSPE g c t a c c c t t g t g g g c c c c c c g g c g a c g c t t c c t g c t c c g c c c c t a a g t c g g g a a g g t t c c c g a t g g g a a c a c c c g g g g g g c c g c t g c g a a g g a c g a g g c g g g g a t t c a g c c c t t c c a a g g

SpeI 4080 hPGK promoter LAVRGVPDVTNGSRTSH* KRNAAHRVHCVSAARRVLVR t t g c g g t t c g c g g c g t g c c g g a c g t g a c a a a c g g a a g c c g c a c g t c t c a c t a g t a c c c t c a a c g c c a a g c g c c g c a c g g c c t g c a c t g t t t g c c t t c g g c g t g c a g a g t g a t c a t g g g a g

4140 hPGK promoter MAARRPRWAVAN ASPCRWPAIAARRGRHATAL g c a g a c g g a c a g c g c c a g g g a g c a a t g g c a g c g c g c c g a c c g c g a t g g g c t g t g g c c a a t c g t c t g c c t g t c g c g g t c c c t c g t t a c c g t c g c g c g g c t g g c g c t a c c c g a c a c c g g t t a

AscI 4200 hPGK promoter SGCSAGRAESSGREGAVREA LPQEAPRASLLPRSPATRSA *CPAGLAAAPFPRHPLR a g c g g c t g c t c a g c a g g g c g c g c c g a g a g c a g c g g c c g g g a a g g g g c g g t g c g g g a g g c g t c g c c g a c g a g t c g t c c c g c g c g g c t c t c g t c g c c g g c c c t t c c c c g c c a c g c c c t c c g c

4260 hPGK promoter G T G T T C C G C A T T C T G C A A G hPGK-F GCGAVVWALFLPARCSAFCK PHPATTHARNRGARHEANQL PTPRYHPGQEQGRPTGCEAL g g g t g t g g g g c g g t a g t g t g g g c c c t g t t c c t g c c c g c g c g g t g t t c c g c a t t c t g c a a g c c c a c a c c c c g c c a t c a c a c c c g g g a c a a g g a c g g g c g c g c c a c a a g g c g t a a g a c g t t c

4320 hPGK promoter PPERTSAVGSLVDRITDLSP GGSRVDATPERTSRIVSREG RRLARRCDAGENVSDGVERG c c t c c g g a g c g c a c g t c g g c a g t c g g c t c c c t c g t t g a c c g a a t c a c c g a c c t c t c t c c c g g a g g c c t c g c g t g c a g c c g t c a g c c g a g g g a g c a a c t g g c t t a g t g g c t g g a g a g a g g g

BamHI 4380 1 5 10 MTEYKPTVRLAT hPGK promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R QGDPPELTMTEYKPTVRLAT WPSGGSSVM LPIWRLKGHGLVLGRHAEGG c a g g g g g a t c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c g t c c c c c t a g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g

BsiWITth111IPflFI 4440 15 20 25 30 RDDVPRAVRTLAAAFADYPA PuroR RDDVPRAVRTLAAAFADYPA AVVDGPGYAGEGGREGVVGG c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g

BstEIIRsrII 4500 35 40 45 50 TRHTVDPDRHIERVTELQEL PuroR TRHTVDPDRHIERVTELQEL RAVGDIRVAVDLPDGLQLFE a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g

4560 55 60 65 70 FLTRVGLDIGKVWVADDGAA PuroR FLTRVGLDIGKVWVADDGAA EERADPEVDALHPDRVVAGR t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c

4620 75 80 85 90 VAVWTTPESVEAGAVFAEIG PuroR VAVWTTPESVEAGAVFAEIG HRDPGRRLADFRPRHEGLDA g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g

4680 95 100 105 110 PRMAELSGSRLAAQQQMEGL PuroR PRMAELSGSRLAAQQQMEGL RAHGLQATGPQGRLLLHFAE c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g

4740 115 120 125 130 LAPHRPKEPAWFLATVGVSP PuroR LAPHRPKEPAWFLATVGVSP QRRVPGLLGRPEQGGDADRG c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g

4800 135 140 145 150 DHQGKGLGSAVVLPGVEAAE PuroR DHQGKGLGSAVVLPGVEAAE VVLALTQAAGDHEGSHLRGL g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c

4860 155 160 165 170 RAGVPAFLETSAPRNLPFYE PuroR G C A A C C T C C C C T T C T A C G A G Puro-F RAGVPAFLETSAPRNLPFYE AGPHGGEQLGGRGAVEGEVL c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c

4920 175 180 185 190 RLGFTVTADVEVPEGPRTWC PuroR C Puro-F RLGFTVTADVEVPEGPRTWC PEAEGDGGVDLHGFSRAGPA c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g

4980 195 200 MTRKPGA* PuroR MTRKPGA* HGALGTGSARGVVRLARGFP a t g a c c c g c a a g c c c g g t g c c t g a c g c c c g c c c c a c g a c c c g c a g c g c c c g a c c g a a a g g t a c t g g g c g t t c g g g c c a c g g a c t g c g g g c g g g g t g c t g g g c g t c g c g g g c t g g c t t t c c

NsiI 5040 ACSGM a g c g c a c g a c c c c a t g c a t c g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t t c g c g t g c t g g g g t a c g t a g c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a

5100 3' LTR ( Δ U3) c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t

5160 3' LTR ( Δ U3) c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c

5220 3' LTR ( Δ U3) c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a

5280 3' LTR ( Δ U3) c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a

5340 3' LTR ( Δ U3) t a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a a t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t

5400 SV40 poly(A) signal t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a

5460 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a

5520 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g

5580 SV40 ori c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g

SfiI 5640 SV40 ori a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a

AvrII 5700 SV40 ori T7 promoter G G G A T A T C T7 t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c

5760 T7 promoter M13 fwd A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g

5820 t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c

5880 f1 ori c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t

5940 f1 ori c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g

6000 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g

6060 f1 ori g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c

6120 f1 ori t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g

6180 f1 ori G T G G F1ori-F a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c

6240 f1 ori A C T C T T G T T C C A A A C T G G F1ori-F a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t

6300 f1 ori a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t

6360 f1 ori c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c

6420 AmpR promoter c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t

6480 1 5 signal sequence MSIQH AmpR promoter AmpR MSIQH c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a

6540 10 15 20 signal sequence FRVALIPFFAAFCLPVFA 25 HP AmpR FRVALIPFFAAFCLPVFAHP t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t

6600 30 35 40 45 ETLVKVKDAEDQLGARVGYI AmpR ETLVKVKDAEDQLGARVGYI g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g

6660 50 55 60 65 ELDLNSGKILESFRPEERFP AmpR ELDLNSGKILESFRPEERFP g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t

6720 70 75 80 85 MMSTFKVLLCGAVLSRIDAG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MMSTFKVLLCGAVLSRIDAG a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c

ScaI 6780 90 95 100 105 QEQLGRRIHYSQNDLVEYSP AmpR QEQLGRRIHYSQNDLVEYSP c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t

6840 110 115 120 125 VTEKHLTDGMTVRELCSAAI AmpR VTEKHLTDGMTVRELCSAAI g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t

6900 130 135 140 145 TMSDNTAANLLLTTIGGPKE AmpR TMSDNTAANLLLTTIGGPKE a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c

6960 150 155 160 165 LTAFLHNMGDHVTRLDRWEP AmpR LTAFLHNMGDHVTRLDRWEP c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c

7020 170 175 180 185 ELNEAIPNDERDTTMPVAMA AmpR ELNEAIPNDERDTTMPVAMA g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t

7080 190 195 200 205 TTLRKLLTGELLTLASRQQL AmpR TTLRKLLTGELLTLASRQQL a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t

7140 210 215 220 225 IDWMEADKVAGPLLRSALPA AmpR IDWMEADKVAGPLLRSALPA a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a

7200 230 235 240 245 GWFIADKSGAGERGSRGIIA AmpR GWFIADKSGAGERGSRGIIA g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t

7260 250 255 260 265 ALGPDGKPSRIVVIYTTGSQ AmpR ALGPDGKPSRIVVIYTTGSQ g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c

7320 270 275 280 285 ATMDERNRQIAEIGASLIKH AmpR ATMDERNRQIAEIGASLIKH g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a

7380 W* AmpR W* t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a

7440 t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t

7500 ori c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t

7560 ori g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c

7620 ori g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g

7680 ori a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c

7740 ori a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g

7800 ori a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c

7860 ori c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g

7920 ori a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t

7980 ori a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a

8040 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c

8100 ori c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c

8160 g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t

8220 t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g

8280 A G C G A G T C A G T G A G C G A G L4440 a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g

8340 a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g

8400 CAP binding site g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t

8460 -35 -10 lac promoter lac operator A G C G G A T A A M13/pUC Reverse c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t

8520 lac operator M13 rev T3 promoter C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T3 c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g

8580 T3 promoter RSV promoter T A A A G G T3 t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t a t t t c c c t t g t t t t c g a c c t c g a c g t t c g a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a

8640 RSV promoter c t t g c a a c a t g g t a a c g a t g a g t t a g c a a c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g g a a c g t t g t a c c a t t g c t a c t c a a t c g t t g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c

SphI 8700 RSV promoter c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t a c g g c t a a c c a c c t t c a t t c c a c c a t g c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c

8760 RSV promoter g t c t g a c a t g g a t t g g a c g a a c c a c t g a a t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t c a g a c t g t a c c t a a c c t g c t t g g t g a c t t a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a

8820 RSV promoter 5' LTR (truncated) g c c t a g c t c g a t a c a t a a a c g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c g g a t c g a g c t a t g t a t t t g c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a

8880 5' LTR (truncated) c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c

8940 5' LTR (truncated) t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a

9000 5' LTR (truncated) c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g

9060 HIV-1 Ψ a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c

9120 HIV-1 Ψ g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c

NruI* 9180 HIV-1 Ψ g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a

9240 a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a

9300 a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a

9360 g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g

9420 a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a

9480 a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t

MfeI 9540 MRDNWRSELYKY t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t

9600 KVVKIEPLGVAPTKAKRRVV a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g

9660 RRE QREKRAVGIGALFLGFLGAA a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c

9720 RRE GSTMGAASMTLTVQARQLLS g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c

BbvCI 9780 RRE GIVQQQNNLLRAIEAQQHLL g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g

9840 RRE QLTVWGIKQLQARILAVERY a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g

9900 RRE LKDQQLLGIWGCSGKLICTT t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c

9960 AVPWNASWSNKSLEQIWNHT c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t

10,020 TWMEWDREINNYTSLIHSLI c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c

10,080 1 5 10 KNEQELLELDKW gp41 peptide EESQNQQEKNEQELLELDKW a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c

10,140 15 ASL (in frame with gp41 peptide) WNWFNITNWLWYIKLFI gp41 peptide ASLWNWFNITNWLWYIKLFI c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t

10,200 (in frame with gp41 peptide) MIVGGLVGLRIVFAVLSIVN MIVGGLVGLRIVFAVLSIVN t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t

KflI 10,260 (in frame with gp41 peptide) RVRQGYSPLSFQTHLPTPRG RVRQGYSPLSFQTHLPTPRG g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g

10,320 (in frame with gp41 peptide) PDRPEGIEEEGGERDRDRSI PDRPEGIEEEGGERDRDRSI c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g

BspDI*ClaI* 10,380 (in frame with gp41 peptide) RLVNGSRRYRSRD* RLVNGSRRYRSRD* g a t t a g t g a a c g g a t c t c g a c g g t a t c g a t c a c g a g a c t a g c c t c g a g c g g c c g c c c c c t c t a a t c a c t t g c c t a g a g c t g c c a t a g c t a g t g c t c t g a t c g g a g c t c g c c g g c g g g g g a

10,440 G A G G G C C T A T T T C C C A T G A T T hU6-F t c a c c g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a a g t g g c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t

10,500 g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g

3ʹ 5ʹ 10,560 g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.