Addgene pMSCVbsd-PPARD-A404W Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pMSCVbsd-PPARD-A404W Sequencing Result


Map View

SgrAI (7158) pBRrevBam (7116 .. 7135) M13/pUC Forward (6966 .. 6988) NdeI (6786) pRS-marker (6753 .. 6772) pGEX 3' (6631 .. 6653) pBRforEco (6575 .. 6593) Amp-R (6165 .. 6184) pBR322ori-F (4863 .. 4882) L4440 (4612 .. 4629) BspQI - SapI (4606) CAP binding site lac operator BspDI - ClaI (3874) NruI * (3706) NheI (31) BmtI (35) BbvCI (211) XbaI (221) BsrGI (1285) pLXSN 5' (1335 .. 1357) pBABE 5' (1373 .. 1389) NsiI (1751) BsiWI (1779) SbfI (2638) PaqCI (2641) BglII (2785) PspOMI (2857) ApaI (2861) NotI (2927) PaeR7I - PspXI - XhoI (2933) HpaI (2941) MSCV-rev (2990 .. 3013) AgeI (3065) BlpI (3228) BspEI (3374) BsmI (3386) mPGK-F (3387 .. 3406) HindIII (3466) XcmI (3471) pMSCVbsd-PPARD-A404W 7334 bp

Sequence View

BmtINheI 60 5' LTR a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a g c t a g c t t a a g t a a c g c c a t t t t g c a a g g c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t c g a t c g a a t t c a t t g c g g t a a a a c g t t c c g

120 5' LTR a t g g a a a a t a c a t a a c t g a g a a t a g a g a a g t t c a g a t c a a g g t t a g g a a c a g a g a g a c a g t a c c t t t t a t g t a t t g a c t c t t a t c t c t t c a a g t c t a g t t c c a a t c c t t g t c t c t c t g t c

180 5' LTR c a g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t t c c t g c c c c g g c t c a g g g c c a a g t c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a a g g a c g g g g c c g a g t c c c g g t t

XbaIBbvCI 240 5' LTR g a a c a g a t g g t c c c c a g a t g c g g t c c c g c c c t c a g c a g t t t c t a g a g a a c c a t c a g a t g t c t t g t c t a c c a g g g g t c t a c g c c a g g g c g g g a g t c g t c a a a g a t c t c t t g g t a g t c t a c a

300 5' LTR t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c c t g t g c c t t a t t t g a a c t a a c c a a t c a g t a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g g a c a c g g a a t a a a c t t g a t t g g t t a g t c a

360 5' LTR t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t c c c c g a g c t c a a t a a a a g a g c c c a c a a c a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a g g g g c t c g a g t t a t t t t c t c g g g t g t t g

420 5' LTR c c c t c a c t c g g c g c g c c a g t c c t c c g a t a g a c t g c g t c g c c c g g g t a c c c g t a t t c c c a a g g g a g t g a g c c g c g c g g t c a g g a g g c t a t c t g a c g c a g c g g g c c c a t g g g c a t a a g g g t t

480 5' LTR t a a a g c c t c t t g c t g t t t g c a t c c g a a t c g t g g a c t c g c t g a t c c t t g g g a g g g t c t c c t a t t t c g g a g a a c g a c a a a c g t a g g c t t a g c a c c t g a g c g a c t a g g a a c c c t c c c a g a g g a

540 5' LTR c a g a t t g a t t g a c t g c c c a c c t c g g g g g t c t t t c a t t t g g a g g t t c c a c c g a g a t t t g g a g t c t a a c t a a c t g a c g g g t g g a g c c c c c a g a a a g t a a a c c t c c a a g g t g g c t c t a a a c c t

600 MESV Ψ g a c c c c t g c c c a g g g a c c a c c g a c c c c c c c g c c g g g a g g t a a g c t g g c c a g c g g t c g t t t c t g g g g a c g g g t c c c t g g t g g c t g g g g g g g c g g c c c t c c a t t c g a c c g g t c g c c a g c a a a

660 MESV Ψ c g t g t c t g t c t c t g t c t t t g t g c g t g t t t g t g c c g g c a t c t a a t g t t t g c g c c t g c g t c t g c a c a g a c a g a g a c a g a a a c a c g c a c a a a c a c g g c c g t a g a t t a c a a a c g c g g a c g c a g a

720 MESV Ψ g t a c t a g t t a g c t a a c t a g c t c t g t a t c t g g c g g a c c c g t g g t g g a a c t g a c g a g t t c t g c a t g a t c a a t c g a t t g a t c g a g a c a t a g a c c g c c t g g g c a c c a c c t t g a c t g c t c a a g a c

780 MESV Ψ a a c a c c c g g c c g c a a c c c t g g g a g a c g t c c c a g g g a c t t t g g g g g c c g t t t t t g t g g c c c t t g t g g g c c g g c g t t g g g a c c c t c t g c a g g g t c c c t g a a a c c c c c g g c a a a a a c a c c g g g

840 MESV Ψ g a c c t g a g g a a g g g a g t c g a t g t g g a a t c c g a c c c c g t c a g g a t a t g t g g t t c t g g t a g g c t g g a c t c c t t c c c t c a g c t a c a c c t t a g g c t g g g g c a g t c c t a t a c a c c a a g a c c a t c c

900 MESV Ψ a g a c g a g a a c c t a a a a c a g t t c c c g c c t c c g t c t g a a t t t t t g c t t t c g g t t t g g a a c c g t c t g c t c t t g g a t t t t g t c a a g g g c g g a g g c a g a c t t a a a a a c g a a a g c c a a a c c t t g g c

960 MESV Ψ a a g c c g c g c g t c t t g t c t g c t g c a g c g c t g c a g c a t c g t t c t g t g t t g t c t c t g t c t g a c t t c g g c g c g c a g a a c a g a c g a c g t c g c g a c g t c g t a g c a a g a c a c a a c a g a g a c a g a c t g

1020 gag (truncated) t g t g t t t c t g t a t t t g t c t g a a a a t t a g g g c c a g a c t g t t a c c a c t c c c t t a a g t t t g a c a c a c a a a g a c a t a a a c a g a c t t t t a a t c c c g g t c t g a c a a t g g t g a g g g a a t t c a a a c t g

1080 gag (truncated) c t t a g g t c a c t g g a a a g a t g t c g a g c g g a t c g c t c a c a a c c a g t c g g t a g a t g t c a a g a a g a a t c c a g t g a c c t t t c t a c a g c t c g c c t a g c g a g t g t t g g t c a g c c a t c t a c a g t t c t t

1140 gag (truncated) g a g a c g t t g g g t t a c c t t c t g c t c t g c a g a a t g g c c a a c c t t t a a c g t c g g a t g g c c g c g c t c t g c a a c c c a a t g g a a g a c g a g a c g t c t t a c c g g t t g g a a a t t g c a g c c t a c c g g c g c

1200 gag (truncated) a g a c g g c a c c t t t a a c c g a g a c c t c a t c a c c c a g g t t a a g a t c a a g g t c t t t t c a c c t g g t c t g c c g t g g a a a t t g g c t c t g g a g t a g t g g g t c c a a t t c t a g t t c c a g a a a a g t g g a c c

1260 gag (truncated) c c c g c a t g g a c a c c c a g a c c a g g t c c c c t a c a t c g t g a c c t g g g a a g c c t t g g c t t t t g a g g g c g t a c c t g t g g g t c t g g t c c a g g g g a t g t a g c a c t g g a c c c t t c g g a a c c g a a a a c t

BsrGI 1320 gag (truncated) c c c c c c t c c c t g g g t c a a g c c c t t t g t a c a c c c t a a g c c t c c g c c t c c t c t t c c t c c a t c g g g g g g a g g g a c c c a g t t c g g g a a a c a t g t g g g a t t c g g a g g c g g a g g a g a a g g a g g t a g

1380 gag (truncated) C C C T T G A A C C T C C T C G T T C G A C C pLXSN 5' C T T T A T C C pBABE 5' c g c c c c g t c t c t c c c c c t t g a a c c t c c t c g t t c g a c c c c g c c t c g a t c c t c c c t t t a t c c g c g g g g c a g a g a g g g g g a a c t t g g a g g a g c a a g c t g g g g c g g a g c t a g g a g g g a a a t a g g

1440 gag (truncated) A G C C C T C A C pBABE 5' a g c c c t c a c t c c t t c t c t a g g c g c c g g a a t t a g a t c c a c t a g t a a c g g c c g c c a g t g t g c t c g g g a g t g a g g a a g a g a t c c g c g g c c t t a a t c t a g g t g a t c a t t g c c g g c g g t c a c a c g

1500 MEQPQE t g g a a t t c g c c c t t a g g t g t g g c g c c g a g g g g a g a t c a g c c a t g g a g c a g c c a c a g g a g g a c c t t a a g c g g g a a t c c a c a c c g c g g c t c c c c t c t a g t c g g t a c c t c g t c g g t g t c c t c c

1560 EAPEVREEEEKEEVAEAEGA a a g c c c c t g a g g t c c g g g a a g a g g a g g a g a a a g a g g a a g t g g c a g a g g c a g a a g g a g c c c t t c g g g g a c t c c a g g c c c t t c t c c t c c t c t t t c t c c t t c a c c g t c t c c g t c t t c c t c g g g

1620 PELNGGPQHALPSSSYTDLS c a g a g c t c a a t g g g g g a c c a c a g c a t g c a c t t c c t t c c a g c a g c t a c a c a g a c c t c t c c c g t c t c g a g t t a c c c c c t g g t g t c g t a c g t g a a g g a a g g t c g t c g a t g t g t c t g g a g a g g g

1680 RSSSPPSLLDQLQMGCDGAS g g a g c t c c t c g c c a c c c t c a c t g c t g g a c c a a c t g c a g a t g g g c t g t g a c g g g g c c t c a t c c t c g a g g a g c g g t g g g a g t g a c g a c c t g g t t g a c g t c t a c c c g a c a c t g c c c c g g a g t a

1740 CGSLNMECRVCGDKASGFHY g c g g c a g c c t c a a c a t g g a g t g c c g g g t g t g c g g g g a c a a g g c a t c g g g c t t c c a c t a c g c g c c g t c g g a g t t g t a c c t c a c g g c c c a c a c g c c c c t g t t c c g t a g c c c g a a g g t g a t g c

BsiWINsiI 1800 GVHACEGCKGFFRRTIRMKL g t g t t c a t g c a t g t g a g g g g t g c a a g g g c t t c t t c c g t c g t a c g a t c c g c a t g a a g c t g g c a c a a g t a c g t a c a c t c c c c a c g t t c c c g a a g a a g g c a g c a t g c t a g g c g t a c t t c g a c c

1860 EYEKCERSCKIQKKNRNKCQ a g t a c g a g a a g t g t g a g c g c a g c t g c a a g a t t c a g a a g a a g a a c c g c a a c a a g t g c c a g t t c a t g c t c t t c a c a c t c g c g t c g a c g t t c t a a g t c t t c t t c t t g g c g t t g t t c a c g g t c a

1920 YCRFQKCLALGMSHNAIRFG a c t g c c g c t t c c a g a a g t g c c t g g c a c t g g g c a t g t c a c a c a a c g c t a t c c g t t t t g g t c t g a c g g c g a a g g t c t t c a c g g a c c g t g a c c c g t a c a g t g t g t t g c g a t a g g c a a a a c c a g

1980 RMPEAEKRKLVAGLTANEGS g g a t g c c g g a g g c t g a g a a g a g g a a g c t g g t g g c a g g g c t g a c t g c a a a c g a g g g g a g c c c c t a c g g c c t c c g a c t c t t c t c c t t c g a c c a c c g t c c c g a c t g a c g t t t g c t c c c c t c g g

2040 QYNPQVADLKAFSKHIYNAY a g t a c a a c c c a c a g g t g g c c g a c c t g a a g g c c t t c t c c a a g c a c a t c t a c a a t g c c t a c c t c a t g t t g g g t g t c c a c c g g c t g g a c t t c c g g a a g a g g t t c g t g t a g a t g t t a c g g a t g g

2100 LKNFNMTKKKARSILTGKAS t g a a a a a c t t c a a c a t g a c c a a a a a g a a g g c c c g c a g c a t c c t c a c c g g c a a a g c c a g c c a c t t t t t g a a g t t g t a c t g g t t t t t c t t c c g g g c g t c g t a g g a g t g g c c g t t t c g g t c g g

2160 HTAPFVIHDIETLWQAEKGL a c a c g g c g c c c t t t g t g a t c c a c g a c a t c g a g a c a t t g t g g c a g g c a g a g a a g g g g c t g g t g t g c c g c g g g a a a c a c t a g g t g c t g t a g c t c t g t a a c a c c g t c c g t c t c t t c c c c g a c c

2220 VWKQLVNGLPPYKEISVHVF t g t g g a a g c a g t t g g t g a a t g g c c t g c c t c c c t a c a a g g a g a t c a g c g t g c a c g t c t t c t a c a c c t t c g t c a a c c a c t t a c c g g a c g g a g g g a t g t t c c t c t a g t c g c a c g t g c a g a a g a

2280 YRCQCTTVETVRELTEFAKS a c c g c t g c c a g t g c a c c a c a g t g g a g a c c g t g c g g g a g c t c a c t g a g t t c g c c a a g a g c a t g g c g a c g g t c a c g t g g t g t c a c c t c t g g c a c g c c c t c g a g t g a c t c a a g c g g t t c t c g t

2340 IPSFSSLFLNDQVTLLKYGV t c c c c a g c t t c a g c a g c c t c t t c c t c a a c g a c c a g g t t a c c c t t c t c a a g t a t g g c g t g c a g g g g t c g a a g t c g t c g g a g a a g g a g t t g c t g g t c c a a t g g g a a g a g t t c a t a c c g c a c g

2400 HEAIFAMLASIVNKDGLLVA a c g a g g c c a t c t t c g c c a t g c t g g c c t c t a t c g t c a a c a a g g a c g g g c t g c t g g t a g c c a t g c t c c g g t a g a a g c g g t a c g a c c g g a g a t a g c a g t t g t t c c t g c c c g a c g a c c a t c g g t

2460 NGSGFVTREFLRSLRKPFSD a c g g c a g t g g c t t t g t c a c c c g t g a g t t c c t g c g c a g c c t c c g c a a a c c c t t c a g t g a t a t g c c g t c a c c g a a a c a g t g g g c a c t c a a g g a c g c g t c g g a g g c g t t t g g g a a g t c a c t a t

2520 IIEPKFEFAVKFNALELDDS t c a t t g a g c c t a a g t t t g a a t t t g c t g t c a a g t t c a a c g c c c t g g a a c t t g a t g a c a g t g a g t a a c t c g g a t t c a a a c t t a a a c g a c a g t t c a a g t t g c g g g a c c t t g a a c t a c t g t c a c

2580 DLALFIAAIILCGDRPGLMN a c c t g g c c c t a t t c a t t g c g g c c a t c a t t c t g t g t g g a g a c c g g c c a g g c c t c a t g a a c g t g g a c c g g g a t a a g t a a c g c c g g t a g t a a g a c a c a c c t c t g g c c g g t c c g g a g t a c t t g c

SbfI 2640 VPRVEAIQDTILRALEFHLQ t t c c a c g g g t g g a g g c t a t c c a g g a c a c c a t c c t g c g t g c c c t c g a a t t c c a c c t g c a g g a a g g t g c c c a c c t c c g a t a g g t c c t g t g g t a g g a c g c a c g g g a g c t t a a g g t g g a c g t c c

PaqCI 2700 ANHPDAQYLFPKLLQKMWDL c c a a c c a c c c t g a t g c c c a g t a c c t c t t c c c c a a g c t g c t g c a g a a g a t g t g g g a c c t g c g g t t g g t g g g a c t a c g g g t c a t g g a g a a g g g g t t c g a c g a c g t c t t c t a c a c c c t g g a c g

2760 RQLVTEHAQMMQRIKKTETE g g c a a c t g g t c a c c g a g c a c g c c c a g a t g a t g c a g c g g a t c a a g a a g a c c g a a a c c g a g a c c g t t g a c c a g t g g c t c g t g c g g g t c t a c t a c g t c g c c t a g t t c t t c t g g c t t t g g c t c t

BglII 2820 TSLHPLLQEIYKDMY* c c t c g c t g c a c c c t c t g c t c c a g g a g a t c t a c a a g g a c a t g t a c t a a c g g c g g c a c c c a g g g a g c g a c g t g g g a g a c g a g g t c c t c t a g a t g t t c c t g t a c a t g a t t g c c g c c g t g g g t c

PspOMIApaI 2880 g c c t c c c t g c a g a c t c c a a t g g g g c c a g c a c t g g a g g g g c c c a c c c a c a t g a c t t t t c c a c g g a g g g a c g t c t g a g g t t a c c c c g g t c g t g a c c t c c c c g g g t g g g t g t a c t g a a a a g g t

PaeR7IPspXIXhoINotI 2940 t t g a c c a g c t c t c a a g g g c g a a t t c t g c a g a t a t c c a t c a c a c t g g c g g c c g c t c g a g g t a a c t g g t c g a g a g t t c c c g c t t a a g a c g t c t a t a g g t a g t g t g a c c g c c g g c g a g c t c c a

HpaI 3000 PGK promoter C G T A C G C G A A A MSCV-rev t a a c g a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a t t g c t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a

3060 PGK promoter T C G T C G G G G C G A C MSCV-rev a g c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c t c g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g

AgeI 3120 PGK promoter a t c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t a g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g

3180 PGK promoter t c c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a g g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t

BlpI 3240 PGK promoter ME a a t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a t t a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t

3300 PGK promoter AGRPLGQRPIAALLLRFLGS g c g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a c g c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t

3360 PGK promoter EAGKGWVRGRAQGRAQGRGG g a g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c t c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c

BsmIBspEI 3420 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F RPKVLRRPGILHASKAHVCR c g c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g

HindIIIXcmI 3480 1 MA PGK promoter BSD AVLLFLISGPFDLQPKLTMA g c t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t g c a g c c c a a g c t t a c c a t g g c c c g a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a c g t c g g g t t c g a a t g g t a c c g g

3540 5 10 15 20 KPLSQEESTLIERATATINS BSD KPLSQEESTLIERATATINS a a g c c t t t g t c t c a a g a a g a a t c c a c c c t c a t t g a a a g a g c a a c g g c t a c a a t c a a c a g c t t c g g a a a c a g a g t t c t t c t t a g g t g g g a g t a a c t t t c t c g t t g c c g a t g t t a g t t g t c g

3600 25 30 35 40 IPISEDYSVASAALSSDGRI BSD IPISEDYSVASAALSSDGRI a t c c c c a t c t c t g a a g a c t a c a g c g t c g c c a g c g c a g c t c t c t c t a g c g a c g g c c g c a t c t a g g g g t a g a g a c t t c t g a t g t c g c a g c g g t c g c g t c g a g a g a g a t c g c t g c c g g c g t a g

3660 45 50 55 60 FTGVNVYHFTGGPCAELVVL BSD FTGVNVYHFTGGPCAELVVL t t c a c t g g t g t c a a t g t a t a t c a t t t t a c t g g g g g a c c t t g t g c a g a a c t c g t g g t g c t g a a g t g a c c a c a g t t a c a t a t a g t a a a a t g a c c c c c t g g a a c a c g t c t t g a g c a c c a c g a c

NruI* 3720 65 70 75 80 GTAAAAAAGNLTCIVAIGNE BSD GTAAAAAAGNLTCIVAIGNE g g c a c t g c t g c t g c t g c g g c a g c t g g c a a c c t g a c t t g t a t c g t c g c g a t c g g a a a t g a g c c g t g a c g a c g a c g a c g c c g t c g a c c g t t g g a c t g a a c a t a g c a g c g c t a g c c t t t a c t c

3780 85 90 95 100 NRGILSPCGRCRQVLLDLHP BSD NRGILSPCGRCRQVLLDLHP a a c a g g g g c a t c t t g a g c c c c t g c g g a c g g t g c c g a c a g g t g c t t c t c g a t c t g c a t c c t t t g t c c c c g t a g a a c t c g g g g a c g c c t g c c a c g g c t g t c c a c g a a g a g c t a g a c g t a g g a

3840 105 110 115 120 GIKAIVKDSDGQPTAVGIRE BSD GIKAIVKDSDGQPTAVGIRE g g g a t c a a a g c c a t a g t g a a g g a c a g t g a t g g a c a g c c g a c g g c a g t t g g g a t t c g t g a a c c c t a g t t t c g g t a t c a c t t c c t g t c a c t a c c t g t c g g c t g c c g t c a a c c c t a a g c a c t t

BspDIClaI 3900 125 130 LLPSGYVWEG* BSD LLPSGYVWEG* t t g c t g c c c t c t g g t t a t g t g t g g g a g g g c t a a t c g a t a a a a t a a a a g a t t t t a t t t a g t a a c g a c g g g a g a c c a a t a c a c a c c c t c c c g a t t a g c t a t t t t a t t t t c t a a a a t a a a t c a

3960 c t c c a g a a a a a g g g g g g a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a g c t a g a g a a c c a t g a g g t c t t t t t c c c c c c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t c g a t c t c t t g g t a

4020 c a g a t g t t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c c t g t g c c t t a t t t g a a c t a a c c g t c t a c a a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g g a c a c g g a a t a a a c t t g a t t g g

4080 a a t c a g t t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t c c c c g a g c t c a a t a a a a g a g c t t a g t c a a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a g g g g c t c g a g t t a t t t t c t c g

4140 c c a c a a c c c c t c a c t c g g c g c g c c a g t c c t c c g a t a g a c t g c g t c g c c c g g g t a c c c g t g g g t g t t g g g g a g t g a g c c g c g c g g t c a g g a g g c t a t c t g a c g c a g c g g g c c c a t g g g c a c

4200 t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t g t g g t c t c g c t g t t c c t t g g g a g g g a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a c a c c a g a g c g a c a a g g a a c c c t c c c

4260 t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g t c t t t c a t g g g t a a c a g t t t c t t g a a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c a g a a a g t a c c c a t t g t c a a a g a a c t

4320 a g t t g g a g a a c a a c a t t c t g a g g g t a g g a g t c g a a t a t t a a g t a a t c c t g a c t c a a t t a g t c a a c c t c t t g t t g t a a g a c t c c c a t c c t c a g c t t a t a a t t c a t t a g g a c t g a g t t a a t c

4380 c c a c t g t t t t g a a t c c a c a t a c t c c a a t a c t c c t g a a a t a g t t c a t t a t g g a c a g c g c a g g g t g a c a a a a c t t a g g t g t a t g a g g t t a t g a g g a c t t t a t c a a g t a a t a c c t g t c g c g t c

4440 -10 lac operator lac promoter a a a g a g c t g g g g a g a a t t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c t t t c t c g a c c c c t c t t a a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g

4500 -35 lac promoter CAP binding site c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g

4560 g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a

BspQISapI 4620 G A G C G A G T G L4440 c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g

4680 A C T G A G C G A L4440 t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a

4740 a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t

4800 ori g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g

4860 ori c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a

4920 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a

4980 ori g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c

5040 ori c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t

5100 ori c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t

5160 ori c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g

5220 ori g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c

5280 ori a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c

5340 ori t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t

5400 ori g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g

5460 t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c

5520 g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t

5580 285 280 275 *WHKILSAGIEAI AmpR *WHKILSAGIEAI t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a

5640 270 265 260 255 QRNREDMTAQSGTTYIVVIR AmpR QRNREDMTAQSGTTYIVVIR c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c

5700 250 245 240 235 SPKGDPGLAAIIGRSGREGA AmpR SPKGDPGLAAIIGRSGREGA g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g

5760 230 225 220 215 GSKDAIFWGAPLASRLLPGA AmpR GSKDAIFWGAPLASRLLPGA t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g

5820 210 205 200 195 VKDAEMWDILQQRSALTLLE AmpR VKDAEMWDILQQRSALTLLE a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g

5880 190 185 180 175 GTLLKRLTTAMAVPMTTDRE AmpR GTLLKRLTTAMAVPMTTDRE g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g

5940 170 165 160 155 DNPIAENLEPEWRDLRTVHD AmpR DNPIAENLEPEWRDLRTVHD g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g

6000 150 145 140 135 GMNHLFATLEKPGGITTLLL AmpR GMNHLFATLEKPGGITTLLL c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t

6060 130 125 120 115 NAATNDSMTIAASCLERVTM AmpR NAATNDSMTIAASCLERVTM g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a

6120 110 105 100 95 GDTLHKETVPSYEVLDNQSY AmpR GDTLHKETVPSYEVLDNQSY g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t

6180 90 85 80 75 HIRRGLQEQGADIRSLVAGC AmpR A T A A T A C C G C G C C A C A Amp-R HIRRGLQEQGADIRSLVAGC g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t

6240 70 65 60 55 LLVKFTSMMPFREEPRFSEL AmpR T A G C Amp-R LLVKFTSMMPFREEPRFSEL t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c

6300 50 45 40 35 IKGSNLDLEIYGVRAGLQDE AmpR IKGSNLDLEIYGVRAGLQDE g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g

6360 30 25 ADKVKVLTEPH 20 15 signal sequence AFVPLCFAA AmpR ADKVKVLTEPHAFVPLCFAA a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g

6420 10 5 1 signal sequence FFPILAVRFHQISM AmpR AmpR promoter FFPILAVRFHQISM a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t

6480 AmpR promoter t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t

6540 AmpR promoter g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t

6600 A A T A G G C G T A T C A C G A G G C pBRforEco a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c

6660 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t a g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a

6720 c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c

6780 C G G C A T C A G A G C A G A T T G T A pRS-marker t g t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t a c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a

NdeI 6840 g c a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c g t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c

6900 c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a

6960 a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c

7020 C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c g c a a g g a a t g g t g c a t g c a a g g a g a t g c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g c g t t c c t t a c c a c g t a c g t t c c t c t a c

7080 g c g c c c a a c a g t c c c c c g g c c a c g g g g c c t g c c a c c a t a c c c a c g c c g a a a c a a g c g c t c c g c g g g t t g t c a g g g g g c c g g t g c c c c g g a c g g t g g t a t g g g t g c g g c t t t g t t c g c g a g

7140 G G T G A T G T C G G C G A T A T A G G pBRrevBam a t g a g c c c g a a g t g g c g a g c c c g a t c t t c c c c a t c g g t g a t g t c g g c g a t a t a g g c g c c a t a c t c g g g c t t c a c c g c t c g g g c t a g a a g g g g t a g c c a c t a c a g c c g c t a t a t c c g c g g t

SgrAI 7200 g c a a c c g c a c c t g t g g c g c c g g t g a t g c c g g c c a c g a t g c g t c c g g c g t a g a g g c g a t t a c g t t g g c g t g g a c a c c g c g g c c a c t a c g g c c g g t g c t a c g c a g g c c g c a t c t c c g c t a a t

7260 g t c c a a t t t g t t a a a g a c a g g a t a t c a g t g g t c c a g g c t c t a g t t t t g a c t c a a c a a t a t c a g g t t a a a c a a t t t c t g t c c t a t a g t c a c c a g g t c c g a g a t c a a a a c t g a g t t g t t a t a

7320 c a c c a g c t g a a g c c t a t a g a g t a c g a g c c a t a g a t a a a a t a a a a g a t t t t a t t t a g t c t c g t g g t c g a c t t c g g a t a t c t c a t g c t c g g t a t c t a t t t t a t t t t c t a a a a t a a a t c a g a g

7334 c a g a a a a a g g g g g g g t c t t t t t c c c c c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.