Addgene miniSOG-H2B-C-10 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: miniSOG-H2B-C-10 Sequencing Result


Map View

PciI (4591) pBR322ori-F (4436 .. 4455) ApaLI (4277) EcoO109I (3771) BsaI (3662) TK-pA-R (3527 .. 3546) BstBI (3355) Neo-F (3209 .. 3228) RsrII (3189) PflFI - Tth111I (2791) FspI (2775) PluTI (2676) SfoI (2674) NarI (2673) KasI (2672) Neo-R (2599 .. 2618) BspDI * - ClaI * (2513) StuI (2494) AseI (4656) NdeI (203) SnaBI (309) CMV-F (488 .. 508) NheI (560) BmtI (564) AgeI (569) PstI (712) NruI (751) Eco53kI (866) SacI (868) BglII (909) BsrGI (1051) BstXI (1103) EcoRI (1115) PaqCI (1140) XbaI * (1320) BclI * (1330) MfeI (1423) HincII - HpaI (1436) SV40pA-R (1474 .. 1493) BtsI - BtsαI (1512) EBV-rev (1528 .. 1547) MluI (1559) F1ori-F (1702 .. 1723) F1ori-R (1914 .. 1933) pBABE 3' (2176 .. 2196) CsiI - SexAI * (2262) miniSOG-H2B-C-10 4680 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a

120 CMV enhancer g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t

180 CMV enhancer a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g

NdeI 240 CMV enhancer t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a

300 CMV enhancer a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c

SnaBI 360 CMV enhancer CMV promoter t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a

420 CMV promoter g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a

480 CMV promoter g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a

AgeIBmtINheI 600 1 5 MEKSFV miniSOG Kozak sequence MEKSFV *RDGGHLFTEH t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g t c g c c a c c a t g g a g a a a a g t t t c g t g a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c a g c g g t g g t a c c t c t t t t c a a a g c a c t

660 10 15 20 25 ITDPRLPDNPIIFASDGFLE miniSOG ITDPRLPDNPIIFASDGFLE YSIWPQWVIGDDECGIAEQL t a a c t g a t c c a c g g c t g c c a g a c a a t c c c a t c a t c t t c g c a t c c g a t g g c t t c c t g g a g c a t t g a c t a g g t g c c g a c g g t c t g t t a g g g t a g t a g a a g c g t a g g c t a c c g a a g g a c c t c g

PstI 720 30 35 40 45 LTEYSREEILGRNGRFLQGP miniSOG LTEYSREEILGRNGRFLQGP QGLIGSLLDQAAIAAKQLSW t g a c c g a g t a t t c c a g a g a g g a g a t c c t g g g c c g c a a t g g c c g c t t t c t g c a g g g a c c a g a c t g g c t c a t a a g g t c t c t c c t c t a g g a c c c g g c g t t a c c g g c g a a a g a c g t c c c t g g t c

NruI 780 50 55 60 65 ETDQATVQKIRDAIRDQREI miniSOG ETDQATVQKIRDAIRDQREI LCVLGCHLLNAIGNSILALN a g a c a g a c c a g g c c a c a g t g c a g a a g a t t c g c g a t g c c a t t a g a g a t c a g c g c g a g a t t a t c t g t c t g g t c c g g t g t c a c g t c t t c t a a g c g c t a c g g t a a t c t c t a g t c g c g c t c t a a t

840 70 75 80 85 TVQLINYTKSGKKFWNLLHL miniSOG TVQLINYTKSGKKFWNLLHL GHLQYVVCFAPLFEPVEQVE c c g t g c a g c t g a t a a a c t a c a c a a a a a g c g g g a a g a a a t t c t g g a a c c t c c t g c a c c t c c g g c a c g t c g a c t a t t t g a t g t g t t t t t c g c c c t t c t t t a a g a c c t t g g a g g a c g t g g a g g

Eco53kISacI 900 90 95 100 105 QPMRDQKGELQYFIGVQLDG miniSOG QPMRDQKGELQYFIGVQLDG LGHPVLLTLELIEDSHLQIS a g c c c a t g a g g g a c c a g a a g g g t g a g c t c c a g t a t t t c a t c g g a g t g c a g c t g g a t g g a t t c g g g t a c t c c c t g g t c t t c c c a c t c g a g g t c a t a a a g t a g c c t c a c g t c g a c c t a c c t a

BglII 960 (in frame with miniSOG) SGLRSGGSGG 5 10 PEPAKSAPAP H2B SGLRSGGSGGPEPAKSAPAP GSESRSAGSAWLWRLRSGGR c c g g a c t c a g a t c t g g a g g c t c c g g a g g c c c a g a g c c a g c g a a g t c t g c t c c c g c c c c g a g g c c t g a g t c t a g a c c t c c g a g g c c t c c g g g t c t c g g t c g c t t c a g a c g a g g g c g g g g c t

1020 15 20 25 30 KKGSKKAVTKAQKKGGKKRK H2B KKGSKKAVTKAQKKGGKKRK FLAGLLRHSLRLLFAALLAL a a a a g g g c t c c a a g a a g g c g g t g a c t a a g g c g c a g a a g a a a g g c g g c a a g a a g c g c a a g c t t t t c c c g a g g t t c t t c c g c c a c t g a t t c c g c g t c t t c t t t c c g c c g t t c t t c g c g t t c g

BsrGI 1080 35 40 45 50 RSRKESYSIYVYKVLKQVHP H2B RSRKESYSIYVYKVLKQVHP AAALLAIGDIHVLNQLLDVR g c a g c c g c a a g g a g a g c t a t t c c a t c t a t g t g t a c a a g g t t c t g a a g c a g g t c c a c c c t g c g t c g g c g t t c c t c t c g a t a a g g t a g a t a c a c a t g t t c c a a g a c t t c g t c c a g g t g g g a c

EcoRIBstXI 1140 55 60 65 70 DTGISSKAMGIMNSFVNDIF H2B DTGISSKAMGIMNSFVNDIF VGANRGLGHADHIRKHVVNE a c a c c g g c a t t t c g t c c a a g g c c a t g g g c a t c a t g a a t t c g t t t g t g a a c g a c a t t t t c g t g t g g c c g t a a a g c a g g t t c c g g t a c c c g t a g t a c t t a a g c a a a c a c t t g c t g t a a a a g c

PaqCI 1200 75 80 85 90 ERIAGEASRLAHYNKRSTIT H2B ERIAGEASRLAHYNKRSTIT LADCTLSGAQRMVVLARGDG a g c g c a t c g c a g g t g a g g c t t c c c g c c t g g c g c a t t a c a a c a a g c g c t c g a c c a t c a c c t t c g c g t a g c g t c c a c t c c g a a g g g c g g a c c g c g t a a t g t t g t t c g c g a g c t g g t a g t g g a

1260 95 100 105 110 SREIQTAVRLLLPGELAKHA H2B SREIQTAVRLLLPGELAKHA GPLDLRGHAQQQRPLQGLVG c c a g g g a g a t c c a g a c g g c c g t g c g c c t g c t g c t g c c t g g g g a g t t g g c c a a g c a c g c c g g g t c c c t c t a g g t c t g c c g g c a c g c g g a c g a c g a c g g a c c c c t c a a c c g g t t c g t g c g g c

1320 115 120 125 VSEGTKAITKYTSAK* H2B VSEGTKAITKYTSAK* HGLTSLGDGLVGASLLSGGS t g t c c g a g g g t a c t a a g g c c a t c a c c a a g t a c a c c a g c g c t a a g t a a g g a t c c a c c g g a t a c a g g c t c c c a t g a t t c c g g t a g t g g t t c a t g t g g t c g c g a t t c a t t c c t a g g t g g c c t a

BclI*XbaI* 1380 RSLQDYDAM c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t

HincIIHpaIMfeI 1440 SV40 poly(A) signal a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a

1500 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t

MluIBtsαIBtsI 1560 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a c t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t g

1620 f1 ori g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g

1680 f1 ori t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g

1740 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g

1800 f1 ori t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t

1860 f1 ori c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c

1920 f1 ori A G G G A A G F1ori-R a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c

1980 f1 ori A A A G C G A A A G G A G F1ori-R a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g

2040 f1 ori a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t

2100 AmpR promoter a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g

2160 AmpR promoter SV40 promoter SV40 enhancer a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c

2220 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c

SexAI*CsiI 2280 SV40 promoter SV40 enhancer c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g

2340 SV40 promoter SV40 enhancer c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c

2400 SV40 promoter SV40 enhancer SV40 ori t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g

2460 SV40 promoter SV40 ori c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g

BspDI*ClaI*StuI 2520 SV40 promoter SV40 ori t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t

2580 1 5 10 MIEQDGLHAGSP NeoR/KanR 1 5 10 MIEQDGLHAGSP NeoR/KanR MIEQDGLHAGSP g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c

2640 15 20 25 30 AAWVERLFGYDWAQQTIGCS NeoR/KanR 15 20 25 30 AAWVERLFGYDWAQQTIGCS NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AAWVERLFGYDWAQQTIGCS g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a

KasINarISfoIPluTI 2700 35 40 45 50 DAAVFRLSAQGRPVLFVKTD NeoR/KanR 35 40 45 50 DAAVFRLSAQGRPVLFVKTD NeoR/KanR DAAVFRLSAQGRPVLFVKTD g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g

2760 55 60 65 70 LSGALNELQDEAARLSWLAT NeoR/KanR 55 60 65 70 LSGALNELQDEAARLSWLAT NeoR/KanR LSGALNELQDEAARLSWLAT c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c

Tth111IPflFIFspI 2820 75 80 85 90 TGVPCAAVLDVVTEAGRDWL NeoR/KanR 75 80 85 90 TGVPCAAVLDVVTEAGRDWL NeoR/KanR TGVPCAAVLDVVTEAGRDWL a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c

2880 95 100 105 110 LLGEVPGQDLLSSHLAPAEK NeoR/KanR 95 100 105 110 LLGEVPGQDLLSSHLAPAEK NeoR/KanR LLGEVPGQDLLSSHLAPAEK *RVKSRGLF c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t

2940 115 120 125 130 VSIMADAMRRLHTLDPATCP NeoR/KanR 115 120 125 130 VSIMADAMRRLHTLDPATCP NeoR/KanR VSIMADAMRRLHTLDPATCP YGDHSICHPPQMRKIRSGAW g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t

3000 135 140 145 150 FDHQAKHRIERARTRMEAGL NeoR/KanR 135 140 145 150 FDHQAKHRIERARTRMEAGL NeoR/KanR FDHQAKHRIERARTRMEAGL EVVLRFMADLSCTSPHFGTK t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a

3060 155 160 165 170 VDQDDLDEEHQGLAPAELFA NeoR/KanR 155 160 165 170 VDQDDLDEEHQGLAPAELFA NeoR/KanR VDQDDLDEEHQGLAPAELFA DILIIQVFLMLPERWGFQEG g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g

3120 175 180 185 190 RLKASMPDGEDLVVTHGDAC NeoR/KanR 175 180 185 190 RLKASMPDGEDLVVTHGDAC NeoR/KanR RLKASMPDGEDLVVTHGDAC PELRAHGVALIEDHGMAIGA a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g

3180 195 200 205 210 LPNIMVENGRFSGFIDCGRL NeoR/KanR 195 200 205 210 LPNIMVENGRFSGFIDCGRL NeoR/KanR LPNIMVENGRFSGFIDCGRL QRIDHHFIAAKRSEDVTAPQ t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c

RsrII 3240 215 220 225 230 GVADRYQDIALATRDIAEEL NeoR/KanR 215 220 225 230 GVADRYQDIALATRDIAEEL NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F GVADRYQDIALATRDIAEEL THRVAILVYRQSGTINSFLK g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a

3300 235 240 245 250 GGEWADRFLVLYGIAAPDSQ NeoR/KanR 235 240 245 250 GGEWADRFLVLYGIAAPDSQ NeoR/KanR GGEWADRFLVLYGIAAPDSQ AAFPSVAEEHKVTDGSGIRL g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c

BstBI 3360 255 260 265 RIAFYRLLDEFF* NeoR/KanR 255 260 265 RIAFYRLLDEFF* NeoR/KanR RIAFYRLLDEFF* ADGEIAKKVLEESRSEPTRF c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t

3420 MTDQATPNLPSRDFDSTAAF SRGLSAWGAM t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a

3480 YERLGFGIVFRDAGWMILQR a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c

3540 A C T T T G T G C C T T C C TK-pA-R GDLMLEFFAHPRGRLTETRK g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c

3600 HSV TK poly(A) signal T C T G T T TK-pA-R ETIPEGTRAMTAIKRQNKTH a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c

3660 HSV TK poly(A) signal GVGSFVHKRGVRSQGWHSVD g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t

BsaI 3720 TPPRPHWGQYARVSSFSPPH c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g

EcoO109I 3780 PPSSGEGPGLAANVGAAGPA c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t

3840 IASGYSYIL* t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t

3900 g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a

3960 ori c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a

4020 ori t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a

4080 ori t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t

4140 ori t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c

4200 ori c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t

4260 ori a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c

ApaLI 4320 ori g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t

4380 ori g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t

4440 ori G G G A A pBR322ori-F g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t

4500 ori A C G C C T G G T A T C T T T pBR322ori-F a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a

4560 ori t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g

PciI 4620 g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a

AseI 3ʹ 5ʹ 4680 c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.