Addgene mTagBFP2-TOMM20-N-10 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: mTagBFP2-TOMM20-N-10 Sequencing Result


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SfiI (5091) CsiI - SexAI * (4905) pBABE 3' (4819 .. 4839) F1ori-R (4557 .. 4576) F1ori-F (4345 .. 4366) AflII (4198) EBV-rev (4171 .. 4190) SV40pA-R (4117 .. 4136) HincII - HpaI (4079) XbaI * (3970) NotI (3960) PacI (3955) PshAI (3884) BsmBI - Esp3I (3683) BbsI (3552) BsrGI (3294) AgeI (3232) BamHI (3226) PstI (3102) ApaI (2824) PspOMI (2820) SgrAI (2807) BstXI (2782) BmtI (2772) NheI (2768) CMV-F (2696 .. 2716) BseRI (19) BspDI * - ClaI * (41) Neo-R (127 .. 146) KasI (200) NarI (201) SfoI (202) PluTI (204) FspI (303) PflFI - Tth111I (319) BsrDI (434) RsrII (717) Neo-F (737 .. 756) BstBI (883) TK-pA-R (1055 .. 1074) pBR322ori-F (1964 .. 1983) AflIII - PciI (2119) AseI (2184) NdeI (2411) SnaBI (2517) mTagBFP2-TOMM20-N-10 5115 bp

Sequence View

BspDI*ClaI*BseRI 5ʹ 3ʹ 60 SV40 promoter SV40 ori a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t

120 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR MIEQDGLHAGSPAAWV g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c

180 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R ERLFGYDWAQQTIGCSDAAV g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c

KasINarISfoIPluTI 240 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR FRLSAQGRPVLFVKTDLSGA t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g

300 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR LNELQDEAARLSWLATTGVP c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a

Tth111IPflFIFspI 360 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR CAAVLDVVTEAGRDWLLLGE t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t

420 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR VPGQDLLSSHLAPAEKVSIM *RVKSRGLFYGDH g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c

BsrDI 480 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR ADAMRRLHTLDPATCPFDHQ SICHPPQMRKIRSGAWEVVL g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t

540 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR AKHRIERARTRMEAGLVDQD RFMADLSCTSPHFGTKDILI g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a

600 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR DLDEEHQGLAPAELFARLKA IQVFLMLPERWGFQEGPELR g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c

660 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR SMPDGEDLVVTHGDACLPNI AHGVALIEDHGMAIGAQRID a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g

RsrII 720 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR MVENGRFSGFIDCGRLGVAD HHFIAAKRSEDVTAPQTHRV a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g

780 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F RYQDIALATRDIAEELGGEW AILVYRQSGTINSFLKAAFP c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c

840 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR ADRFLVLYGIAAPDSQRIAF SVAEEHKVTDGSGIRLADGE g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g

BstBI 900 260 265 YRLLDEFF* NeoR/KanR 260 265 YRLLDEFF* NeoR/KanR YRLLDEFF* MTDQ IAKKVLEESRSEPTRFSRGL t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c

960 ATPNLPSRDFDSTAAFYERL SAWGAM c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c

1020 GFGIVFRDAGWMILQRGDLM g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g

1080 A C T T T G T G C C T T C C T C T G T T TK-pA-R LEFFAHPRGRLTETRKETIP t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c

1140 HSV TK poly(A) signal EGTRAMTAIKRQNKTHGVGS a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a

1200 HSV TK poly(A) signal FVHKRGVRSQGWHSVDTPPR t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g

1260 PHWGQYARVSSFSPPHPPSS c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c

1320 GEGPGLAANVGAAGPAIASG g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a

1380 YSYIL* a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t

1440 a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c

1500 ori c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t

1560 ori t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c

1620 ori a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c

1680 ori t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a

1740 ori a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t

1800 ori c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c

1860 ori g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g

1920 ori g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t

1980 ori G G G A A A C G C C T G G T A T C pBR322ori-F g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g

2040 ori T T T pBR322ori-F t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a

2100 ori c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a

AflIIIPciI 2160 t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g

AseI 2220 g t a t t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c a t a a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g

2280 CMV enhancer c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g

2340 CMV enhancer a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c

2400 CMV enhancer a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a

NdeI 2460 CMV enhancer c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g

SnaBI 2520 CMV enhancer t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t

2580 CMV enhancer CMV promoter t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c

2640 CMV promoter c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a

2700 CMV promoter C G C A A CMV-F t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t

2760 CMV promoter A T G G G C G G T A G G C G T G CMV-F a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a

SgrAIBstXIBmtINheI 2820 Kozak sequence MVGRNSAIAAGVCG c a g a t c c g c t a g c g c c a c c a t g g t g g g t c g g a a c a g c g c c a t c g c c g c c g g t g t a t g c g g g t c t a g g c g a t c g c g g t g g t a c c a c c c a g c c t t g t c g c g g t a g c g g c g g c c a c a t a c g c c

PspOMIApaI 2880 ALFIGYCIYFDRKRRSDPNF g g c c c t t t t c a t t g g g t a c t g c a t c t a c t t c g a c c g c a a a a g a c g a a g t g a c c c c a a c t t c c g g g a a a a g t a a c c c a t g a c g t a g a t g a a g c t g g c g t t t t c t g c t t c a c t g g g g t t g a a

2940 KNRLRERRKKQKLAKERAGL c a a g a a c a g g c t t c g a g a a c g a a g a a a g a a a c a g a a g c t t g c c a a g g a g a g a g c t g g g c t g t t c t t g t c c g a a g c t c t t g c t t c t t t c t t t g t c t t c g a a c g g t t c c t c t c t c g a c c c g a

3000 SKLPDLKDAEAVQKFFLEEI t t c c a a g t t a c c t g a c c t t a a a g a t g c t g a a g c t g t t c a g a a g t t c t t c c t t g a a g a a a t a a g g t t c a a t g g a c t g g a a t t t c t a c g a c t t c g a c a a g t c t t c a a g a a g g a a c t t c t t t a

3060 QLGEELLAQGEYEKGVDHLT a c a g c t t g g t g a a g a g t t a c t a g c t c a a g g t g a a t a t g a g a a g g g c g t a g a c c a t c t g a c t g t c g a a c c a c t t c t c a a t g a t c g a g t t c c a c t t a t a c t c t t c c c g c a t c t g g t a g a c t g

PstI 3120 NAIAVCGQPQQLLQVLQQTL *LLSK a a a t g c a a t t g c t g t g t g t g g a c a g c c a c a g c a g t t a c t g c a g g t c t t a c a g c a a a c t c t t t t a c g t t a a c g a c a c a c a c c t g t c g g t g t c g t c a a t g a c g t c c a g a a t g t c g t t t g a g a

3180 PPPVFQMLLTKLPTISQRIV WWWHELHKQSLEWCNTLSNY t c c a c c a c c a g t g t t c c a g a t g c t t c t g a c t a a g c t c c c a a c a a t t a g t c a g a g a a t t g t a g g t g g t g g t c a c a a g g t c t a c g a a g a c t g a t t c g a g g g t t g t t a a t c a g t c t c t t a a c a

AgeIBamHI 3240 Kozak sequence SAQSLAEDDVEGGSGDPPVA TSLAQSFIIHFATAPIWRDG a a g t g c t c a g a g c t t g g c t g a a g a t g a t g t g g a a g g c g g t a g c g g g g a t c c a c c g g t c g c t t c a c g a g t c t c g a a c c g a c t t c t a c t a c a c c t t c c g c c a t c g c c c c t a g g t g g c c a g c g

BsrGI 3300 5 10 15 20 MVSKGEELIKENMHMKLYM mTagBFP2 Kozak sequence TMVSKGEELIKENMHMKLYM GHHRLAFLQNLLVHVHLQVH c a c c a t g g t g t c t a a g g g c g a a g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a c a t g t g g t a c c a c a g a t t c c c g c t t c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a t g t a

3360 25 30 35 40 EGTVDNHHFKCTSEGEGKPY mTagBFP2 EGTVDNHHFKCTSEGEGKPY LAGHVVMVELACGLAFALGV g g a g g g c a c c g t g g a c a a c c a t c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c a a g c c c t a c c t c c c g t g g c a c c t g t t g g t a g t g a a g t t c a c g t g t a g g c t c c c g c t t c c g t t c g g g a t

3420 45 50 55 60 EGTQTMRIKVVEGGPLPFAF mTagBFP2 EGTQTMRIKVVEGGPLPFAF LAGLGHSDLHDLAAREGEGE c g a g g g c a c c c a g a c c a t g a g a a t c a a g g t g g t c g a g g g c g g c c c t c t c c c c t t c g c c t t g c t c c c g t g g g t c t g g t a c t c t t a g t t c c a c c a g c t c c c g c c g g g a g a g g g g a a g c g g a a

3480 65 70 75 80 DILATSFLYGSKTFINHTQG mTagBFP2 DILATSFLYGSKTFINHTQG VDQSSAEEVAALGEDVVGLA c g a c a t c c t g g c t a c t a g c t t c c t c t a c g g c a g c a a g a c c t t c a t c a a c c a c a c c c a g g g g c t g t a g g a c c g a t g a t c g a a g g a g a t g c c g t c g t t c t g g a a g t a g t t g g t g t g g g t c c c

3540 85 90 95 100 IPDFFKQSFPEGFTWERVTT mTagBFP2 IPDFFKQSFPEGFTWERVTT DGVEELLGERLAECPLSDGC c a t c c c c g a c t t c t t c a a g c a g t c c t t c c c t g a g g g c t t c a c a t g g g a g a g a g t c a c c a c g t a g g g g c t g a a g a a g t t c g t c a g g a a g g g a c t c c c g a a g t g t a c c c t c t c t c a g t g g t g

BbsI 3600 105 110 115 120 YEDGGVLTATQDTSLQDGCL mTagBFP2 YEDGGVLTATQDTSLQDGCL VFVPAHQGSGLVGAELVAAE a t a c g a a g a c g g g g g c g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g c c t t a t g c t t c t g c c c c c g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a c g g a

3660 125 130 135 140 IYNVKIRGVNFTSNGPVMQK mTagBFP2 IYNVKIRGVNFTSNGPVMQK DVVDLDSPHVECGVARHHLL c a t c t a c a a c g t c a a g a t c a g a g g g g t g a a c t t c a c a t c c a a c g g c c c t g t g a t g c a g a a g t a g a t g t t g c a g t t c t a g t c t c c c c a c t t g a a g t g t a g g t t g c c g g g a c a c t a c g t c t t

BsmBIEsp3I 3720 145 150 155 160 KTLGWEAFTETLYPADGGLE mTagBFP2 KTLGWEAFTETLYPADGGLE FCEAPLGEGLRQVGSVAAQF g a a a a c a c t c g g c t g g g a g g c c t t c a c c g a g a c g c t g t a c c c c g c t g a c g g c g g c c t g g a c t t t t g t g a g c c g a c c c t c c g g a a g t g g c t c t g c g a c a t g g g g c g a c t g c c g c c g g a c c t

3780 165 170 175 180 GRNDMALKLVGGSHLIANAK mTagBFP2 GRNDMALKLVGGSHLIANAK ASVVHGQLEHAPAM a g g c a g a a a c g a c a t g g c c c t g a a g c t c g t g g g c g g g a g c c a t c t g a t c g c a a a c g c c a a t c c g t c t t t g c t g t a c c g g g a c t t c g a g c a c c c g c c c t c g g t a g a c t a g c g t t t g c g g t t

3840 185 190 195 200 TTYRSKKPAKNLKMPGVYYV mTagBFP2 TTYRSKKPAKNLKMPGVYYV g a c c a c a t a t a g a t c c a a g a a a c c c g c t a a g a a c c t c a a g a t g c c t g g c g t c t a c t a t g t c t g g t g t a t a t c t a g g t t c t t t g g g c g a t t c t t g g a g t t c t a c g g a c c g c a g a t g a t a c a

PshAI 3900 205 210 215 220 DYRLERIKEANNETYVEQHE mTagBFP2 DYRLERIKEANNETYVEQHE g g a c t a c a g a c t g g a a a g a a t c a a g g a g g c c a a c a a c g a g a c c t a c g t c g a g c a g c a c g a c c t g a t g t c t g a c c t t t c t t a g t t c c t c c g g t t g t t g c t c t g g a t g c a g c t c g t c g t g c t

PacI 3960 225 230 235 VAVARYCDLPSKLGHKLN * mTagBFP2 VAVARYCDLPSKLGHKLN* g g t g g c a g t g g c c a g a t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a a g c t t a a t t a a g c c c a c c g t c a c c g g t c t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t t c g a a t t a a t t c g

XbaI*NotI 4020 g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t

HincIIHpaI 4080 SV40 poly(A) signal a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t

4140 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t

AflII 4200 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a

4260 SV40 poly(A) signal f1 ori a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g

4320 f1 ori a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c

4380 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c

4440 f1 ori g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t

4500 f1 ori t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t

4560 f1 ori A G G G F1ori-R g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c

4620 f1 ori A A G A A A G C G A A A G G A G F1ori-R a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t

4680 f1 ori a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c

4740 AmpR promoter g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

4800 AmpR promoter SV40 promoter SV40 enhancer c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c

4860 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g

SexAI*CsiI 4920 SV40 promoter SV40 enhancer c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a

4980 SV40 promoter SV40 enhancer c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t

5040 SV40 promoter SV40 enhancer SV40 ori t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g

SfiI 5100 SV40 promoter SV40 ori c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c

3ʹ 5ʹ 5115 SV40 promoter SV40 ori a g c t a t t c c a g a a g t t c g a t a a g g t c t t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.