Addgene MTK234_033 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: MTK234_033 Sequencing Result


Map View

BsaAI (2039) MslI (2016) Bpu10I (1956) CAT-R (1861 .. 1880) TsoI (1833) PvuII (1832) BspEI (1732) ApoI (1728) BsrDI (1715) AclI (1643) BpmI (1612) PflMI * (1503) MscI (1465) EaeI (1463) BtgI - NcoI (1427) ScaI (1315) TatI (1313) EcoRV (1280) BanII - SacI (1276) Eco53kI (1274) BanI (1142) BlpI (1081) BspHI (2052) AcuI (136) AlwNI (269) BsiEI (344) ApaLI (364) BseYI (374) PspFI (378) HaeII (438) BssSI - BssSαI (505) pBR322ori-F (523 .. 542) DrdI (576) AflIII - PciI (678) NspI (682) TaqII (714) BamHI (736) NgoMIV (764) NaeI (766) NmeAIII (777) PsiI (963) mU6-F (968 .. 988) MTK234_033 2094 bp

Sequence View

60 ori g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a

120 ori c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t

AcuI 180 ori g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a

240 ori t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t

AlwNI 300 ori c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g

BsiEI 360 ori c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c

BseYIPspFIApaLI 420 ori t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c

HaeII 480 ori t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c

BssSαIBssSI 540 ori G G G A A A C G C C T G G T A T C T pBR322ori-F c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a

DrdI 600 ori T T pBR322ori-F t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g

660 ori a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a

TaqIIAflIIIPciINspI 720 t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c

NgoMIVNaeIBamHI 780 t a g t c g g t c t c a a a c g g a t c c g a c g c g c c a t c t c t a g g c c c g c g c c g g c c c c c t c g c a c g a t c a g c c a g a g t t t g c c t a g g c t g c g c g g t a g a g a t c c g g g c g c g g c c g g g g g a g c g t g c

840 g a c t t g t g g g a g a a g c t c g g c t a c t c c c c t g c c c c g g t t a a t t t g c a t a t a a t a t t t c c t c t g a a c a c c c t c t t c g a g c c g a t g a g g g g a c g g g g c c a a t t a a a c g t a t a t t a t a a a g g a

900 a g t a a c t a t a g a g g c t t a a t g t g c g a t a a a a g a c a g a t a a t c t g t t c t t t t t a a t a c t a g t c a t t g a t a t c t c c g a a t t a c a c g c t a t t t t c t g t c t a t t a g a c a a g a a a a a t t a t g a t c

960 loxP c t a c a t t t t a c a t g a t a g g c t t g g a t t t c t a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g g a t g t a a a a t g t a c t a t c c g a a c c t a a a g a t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c

PsiI 1020 loxP C A G C A C A A A A G G A A A C T C A C C mU6-F t t a t a a a c a g c a c a a a a g g a a a c t c a c c c t a a c t g t a a a g t a a t t g t g t g t t t t g a g a c t a a t a t t t g t c g t g t t t t c c t t t g a g t g g g a t t g a c a t t t c a t t a a c a c a c a a a a c t c t g a

1080 a t a a g t a t c c c t t g g a g a a c c a c c t t g t t t g a t t c c c a g g g c c g g t t a a t g g t t t a a g a g t a t t c a t a g g g a a c c t c t t g g t g g a a c a a a c t a a g g g t c c c g g c c a a t t a c c a a a t t c t c

BlpI 1140 c t a a g c t g g a a a c a g c a t a g c a a g t t t a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g g a t t c g a c c t t t g t c g t a t c g t t c a a a t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c

BanI 1200 lambda t0 terminator t g g c a c c g a g t c g g t g c t t t t t t t g c t g t g a g a c c a g a c c a a t a a a a a a c g c c c g g c g g c a c c g t g g c t c a g c c a c g a a a a a a a c g a c a c t c t g g t c t g g t t a t t t t t t g c g g g c c g c c g

1260 lambda t0 terminator a a c c g a g c g t t c t g a a c a a a t c c a g a t g g a g t t c t g a g g t c a t t a c t g g a t c t a t c a a c a t t g g c t c g c a a g a c t t g t t t a g g t c t a c c t c a a g a c t c c a g t a a t g a c c t a g a t a g t t g t

TatIScaIEco53kIBanIISacIEcoRV 1320 220 215 210 *AGGQWEDCYQ CmR *AGGQWEDCYQ g g a g t c c a a g c g a g c t c g a t a t c a a a t t a c g c c c c g c c c t g c c a c t c a t c g c a g t a c t g t c c t c a g g t t c g c t c g a g c t a t a g t t t a a t g c g g g g c g g g a c g g t g a g t a g c g t c a t g a c a

1380 205 200 195 190 QLENLMRGVHFGDCVAHHVQ CmR QLENLMRGVHFGDCVAHHVQ t g t a a t t c a t t a a g c a t t c t g c c g a c a t g g a a g c c a t c a c a a a c g g c a t g a t g a a c c t g a a c a t t a a g t a a t t c g t a a g a c g g c t g t a c c t t c g g t a g t g t t t g c c g t a c t a c t t g g a c t

NcoIBtgI 1440 185 180 175 170 IALPMLVKDGQTYYKGMTFV CmR IALPMLVKDGQTYYKGMTFV a t c g c c a g c g g c a t c a g c a c c t t g t c g c c t t g c g t a t a a t a t t t g c c c a t g g t g a a a a c g t a g c g g t c g c c g t a g t c g t g g a a c a g c g g a a c g c a t a t t a t a a a c g g g t a c c a c t t t t g c

EaeIMscI 1500 165 160 155 150 PAFFNDMNAVNLDFSTFSVW CmR PAFFNDMNAVNLDFSTFSVW g g g g c g a a g a a g t t g t c c a t a t t g g c c a c g t t t a a a t c a a a a c t g g t g a a a c t c a c c c a g c c c c g c t t c t t c a a c a g g t a t a a c c g g t g c a a a t t t a g t t t t g a c c a c t t t g a g t g g g t c

PflMI* 1560 145 140 135 130 PNASVFFMNEIFGKPFYALN CmR PNASVFFMNEIFGKPFYALN g g a t t g g c t g a a a c g a a a a a c a t a t t c t c a a t a a a c c c t t t a g g g a a a t a g g c c a g g t t t c c t a a c c g a c t t t g c t t t t t g t a t a a g a g t t a t t t g g g a a a t c c c t t t a t c c g g t c c a a a

BpmI 1620 125 120 115 110 EGYCAVDQSYIHLFQRFDDH CmR EGYCAVDQSYIHLFQRFDDH t c a c c g t a a c a c g c c a c a t c t t g c g a a t a t a t g t g t a g a a a c t g c c g g a a a t c g t c g t g g a g t g g c a t t g t g c g g t g t a g a a c g c t t a t a t a c a c a t c t t t g a c g g c c t t t a g c a g c a c c

AclI 1680 105 100 95 90 YESWLSSFTETQEHFVTYCP CmR YESWLSSFTETQEHFVTYCP t a t t c a c t c c a g a g c g a t g a a a a c g t t t c a g t t t g c t c a t g g a a a a c g g t g t a a c a a g g g a t a a g t g a g g t c t c g c t a c t t t t g c a a a g t c a a a c g a g t a c c t t t t g c c a c a t t g t t c c c

BspEIApoIBsrDI 1740 85 80 75 70 HVSDWIVLEGDKMAMRFEPH CmR HVSDWIVLEGDKMAMRFEPH t g a a c a c t a t c c c a t a t c a c c a g c t c a c c g t c t t t c a t t g c c a t a c g a a a t t c c g g a t g a a c t t g t g a t a g g g t a t a g t g g t c g a g t g g c a g a a a g t a a c g g t a t g c t t t a a g g c c t a c t

1800 65 60 55 50 ANMLRALIHIFAPYFKHKNK CmR ANMLRALIHIFAPYFKHKNK g c a t t c a t c a g g c g g g c a a g a a t g t g a a t a a a g g c c g g a t a a a a c t t g t g c t t a t t t t t c c g t a a g t a g t c c g c c c g t t c t t a c a c t t a t t t c c g g c c t a t t t t g a a c a c g a a t a a a a a g

PvuIITsoI 1860 45 40 35 30 KVTKLFATIDLQVTQNYTCQ CmR KVTKLFATIDLQVTQNYTCQ t t t a c g g t c t t t a a a a a g g c c g t a a t a t c c a g c t g a a c g g t c t g g t t a t a g g t a c a t t g a a a a t g c c a g a a a t t t t t c c g g c a t t a t a g g t c g a c t t g c c a g a c c a a t a t c c a t g t a a c t

1920 25 20 15 10 AVSQFAEFHEKRHWQSIDVT CmR G C A A C T G A C T G A A A T G C C T C CAT-R AVSQFAEFHEKRHWQSIDVT g c a a c t g a c t g a a a t g c c t c a a a a t g t t c t t t a c g a t g c c a t t g g g a t a t a t c a a c g g t g c g t t g a c t g a c t t t a c g g a g t t t t a c a a g a a a t g c t a c g g t a a c c c t a t a t a g t t g c c a c

Bpu10I 1980 5 1 TYGTIKKEM CmR TYGTIKKEM g t a t a t c c a g t g a t t t t t t t c t c c a t t t t a g c t t c c t t a g c t c c t g a a a a t c t c g a t a a c c a t a t a g g t c a c t a a a a a a a g a g g t a a a a t c g a a g g a a t c g a g g a c t t t t a g a g c t a t t g

BsaAIMslI 2040 t c a a a a a a t a c g c c c g g t a g t g a t c t t a t t t c a t t a t g g t g a a a g t t g g a a c c t c t t a c g a g t t t t t t a t g c g g g c c a t c a c t a g a a t a a a g t a a t a c c a c t t t c a a c c t t g g a g a a t g c

BspHI 2094 t g c c c g a t c a a t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c a c g g g c t a g t t a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.