Addgene pUS252 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pUS252 Sequencing Result


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PciI (3290) pBR322ori-F (3135 .. 3154) ApaLI (2976) BspHI (2570) BstBI (2378) Neo-F (2232 .. 2251) TsoI (2225) RsrII (2212) MslI (2133) BtgI - NcoI (2128) BanII (2061) BsrDI (1929) NmeAIII (1920) MscI (1778) L4440 (29 .. 46) lac operator BamHI (260) EcoRI (266) XbaI (270) AbsI - PaeR7I - PspXI - XhoI (277) EcoNI (280) NdeI (291) BseRI (295) AgeI (443) BspEI (517) AfeI (648) BspDI - ClaI (663) PflMI (715) MfeI (855) BlpI (914) PstI (1030) SphI (1036) M13 Forward (1047 .. 1064) M13/pUC Forward (1056 .. 1078) PvuI (1164) BglI (1193) NheI (1210) BmtI (1214) BstAPI (1248) XcmI (1512) BglII (1532) EagI (1602) Neo-R (1622 .. 1641) pUS252 3359 bp

Sequence View

60 A G C G A G T C A G T G A G C G A G L4440 c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c

120 c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t

180 CAP binding site c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g

240 -35 -10 CAP binding site lac promoter lac operator *VKHKRSTHQTSNH t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a

BseRINdeIBamHIEcoRIXbaIPaeR7IPspXIXhoIAbsIEcoNI 300 lac operator MVS ASLLKVSGFELGRPPKMHHA g a g c g g a t a a c a a t t t c a c g g a t c c g a a t t c t a g a c c t c g a g g a g g t t t c a t a t g g t g a g c t c g c c t a t t g t t a a a g t g c c t a g g c t t a a g a t c t g g a g c t c c t c c a a a g t a t a c c a c t c

360 SGEDIFSGLVPILIELEGDV TAFINERAQNRNQDLQLAIH c a g t g g c g a a g a t a t t t t c t c g g g c t t g g t t c c g a t t c t g a t c g a g c t g g a g g g c g a t g t g t c a c c g c t t c t a t a a a a g a g c c c g a a c c a a g g c t a a g a c t a g c t c g a c c t c c c g c t a c a

420 NGHRFSVRGEGYGDASNGKL VTMTKANATFTIAVRAVAFQ g a a c g g t c a t c g t t t t a g c g t t c g c g g t g a a g g t t a t g g c g a c g c g a g c a a c g g c a a a c t c t t g c c a g t a g c a a a a t c g c a a g c g c c a c t t c c a a t a c c g c t g c g c t c g t t g c c g t t t g a

AgeI 480 EIKFICTTGRLPVPWPTLVT FNLEDARGTAQRHRPRGQHR g g a a a t t a a g t t c a t c t g c a c g a c c g g t c g c c t g c c g g t g c c t t g g c c g a c c t t g g t g a c c c t t t a a t t c a a g t a g a c g t g c t g g c c a g c g g a c g g c c a c g g a a c c g g c t g g a a c c a c t g

BspEI 540 TLSYGVQCFAKYPEHMRQND GQRIAHLTKRLIRLVHALVI g a c c t t g t c g t a t g g c g t g c a g t g t t t t g c g a a g t a t c c g g a g c a c a t g c g c c a a a a c g a c t g g a a c a g c a t a c c g c a c g t c a c a a a a c g c t t c a t a g g c c t c g t g t a c g c g g t t t t g c t

600 FFKSAMPDGYVQERTISFKE EKFTRHRVTVDLLTGNGELF t t t c t t t a a a a g t g c g a t g c c g g a c g g t t a c g t c c a g g a g c g t a c c a t t t c c t t c a a g g a a a a g a a a t t t t c a c g c t a c g g c c t g c c a a t g c a g g t c c t c g c a t g g t a a a g g a a g t t c c t

AfeI 660 DGTYKTRAEVKFEGEALVNR IARVFSACLNLKFTFRQDIT a g a t g g c a c g t a c a a a a c t c g c g c a g a g g t t a a g t t t g a a g g t g a a g c g c t g g t c a a t c g t c t a c c g t g c a t g t t t t g a g c g c g t c t c c a a t t c a a a c t t c c a c t t c g c g a c c a g t t a g c

PflMIBspDIClaI 720 IDLKGLEFKEDGNILGHKLE DIQLTQLKFLITVNQAMFQL t a t c g a t t t g a a g g g t t t g g a g t t t a a a g a g g a t g g t a a c a t t c t g g g c c a t a a a c t g g a a t a g c t a a a c t t c c c a a a c c t c a a a t t t c t c c t a c c a t t g t a a g a c c c g g t a t t t g a c c t

780 YSFNSHYVYITADKNRNGLE IAEVAMINVNRCVLITVAQL g t a t a g c t t c a a c a g c c a t t a t g t t t a c a t t a c g g c a g a c a a g a a t c g t a a c g g c t t g g a c a t a t c g a a g t t g t c g g t a a t a c a a a t g t a a t g c c g t c t g t t c t t a g c a t t g c c g a a c c t

840 AQFRIRHNVDDGSVQLADHY GLETNAVINIVTADLQGVM g g c c c a a t t c c g t a t t c g c c a c a a t g t t g a t g a c g g t a g c g t c c a a c t g g c c g a c c a t t a c c g g g t t a a g g c a t a a g c g g t g t t a c a a c t a c t g c c a t c g c a g g t t g a c c g g c t g g t a a t

MfeI 900 QQNTPIGEGPVLLPEQHYLT c c a a c a g a a c a c c c c a a t t g g t g a g g g t c c g g t g t t g c t g c c g g a a c a a c a c t a t c t g a c g g t t g t c t t g t g g g g t t a a c c a c t c c c a g g c c a c a a c g a c g g c c t t g t t g t g a t a g a c t g

BlpI 960 TNSVLSKDPQERRDHMVLVE g a c c a a t a g c g t g c t g a g c a a a g a t c c g c a g g a a c g c c g t g a t c a c a t g g t c c t g g t g g a c t g g t t a t c g c a c g a c t c g t t t c t a g g c g t c c t t g c g g c a c t a g t g t a c c a g g a c c a c c t

1020 FVTAAGLSLGMDELYKS* a t t t g t g a c c g c t g c g g g c t t g a g c c t g g g t a t g g a c g a g c t g t a t a a g a g c t a a t g a a a t a a a c a c t g g c g a c g c c c g a a c t c g g a c c c a t a c c t g c t c g a c a t a t t c t c g a t t a c t t t

SphIPstI 1080 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward g c t t g c t g c a g g c a t g c a a g c t t g g c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a c g a a c g a c g t c c g t a c g t t c g a a c c g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t

1140 a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a

BglIPvuI 1200 a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t

BstAPIBmtINheI 1260 t g g c g a t a a g c t a g c t t c a c g c t g c c g c a a g c a c t c a g g g c g c a a g g g c t g c t a a a g g a a a c c g c t a t t c g a t c g a a g t g c g a c g g c g t t c g t g a g t c c c g c g t t c c c g a c g a t t t c c t t

1320 g c g g a a c a c g t a g a a a g c c a g t c c g c a g a a a c g g t g c t g a c c c c g g a t g a a t g t c a g c t a c g c c t t g t g c a t c t t t c g g t c a g g c g t c t t t g c c a c g a c t g g g g c c t a c t t a c a g t c g a t

1380 c t g g g c t a t c t g g a c a a g g g a a a a c g c a a g c g c a a a g a g a a a g c a g g t a g c t t g c a g t g g g a c c c g a t a g a c c t g t t c c c t t t t g c g t t c g c g t t t c t c t t t c g t c c a t c g a a c g t c a c c

1440 g c t t a c a t g g c g a t a g c t a g a c t g g g c g g t t t t a t g g a c a g c a a g c g a a c c g g a a t t g c c c g a a t g t a c c g c t a t c g a t c t g a c c c g c c a a a a t a c c t g t c g t t c g c t t g g c c t t a a c g g

1500 a g c t g g g g c g c c c t c t g g t a a g g t t g g g a a g c c c t g c a a a g t a a a c t g g a t g g c t t t c t t t c g a c c c c g c g g g a g a c c a t t c c a a c c c t t c g g g a c g t t t c a t t t g a c c t a c c g a a a g a a

BglIIXcmI 1560 g c c g c c a a g g a t c t g a t g g c g c a g g g g a t c a a g a t c t g a t c a a g a g a c a g g a t g a g g a t c c g g c g g t t c c t a g a c t a c c g c g t c c c c t a g t t c t a g a c t a g t t c t c t g t c c t a c t c c t a g

EagI 1620 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR MIEQDGLHAGSPAAWVER g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c

1680 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LFGYDWAQQTIGCSDAAVFR g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c

1740 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR LSAQGRPVLFVKTDLSGALN g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t

MscI 1800 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR ELQDEAARLSWLATTGVPCA t g a a c t c c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a c t t g a g g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g

1860 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR AVLDVVTEAGRDWLLLGEVP a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g

NmeAIII 1920 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR GQDLLSSHLAPAEKVSIMAD *RVKSRGLFYGDHSI g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t

BsrDI 1980 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR AMRRLHTLDPATCPFDHQAK CHPPQMRKIRSGAWEVVLRF t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t

2040 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR HRIERARTRMEAGLVDQDDL MADLSCTSPHFGTKDILIIQ a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a

BanII 2100 160 165 170 175 DEEHQGLAPAELFARLKARM NeoR/KanR DEEHQGLAPAELFARLKARM VFLMLPERWGFQEGPELRPH g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g g a t c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c c t a

MslINcoIBtgI 2160 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR PDGEDLVVTHGDACLPNIMV GVALIEDHGMAIGAQRIDHH g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a

RsrII 2220 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR ENGRFSGFIDCGRLGVADRY FIAAKRSEDVTAPQTHRVAI g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t

TsoI 2280 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F QDIALATRDIAEELGGEWAD LVYRQSGTINSFLKAAFPSV t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t

2340 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR RFLVLYGIAAPDSQRIAFYR AEEHKVTDGSGIRLADGEIA c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c

BstBI 2400 260 265 LLDEFF* NeoR/KanR LLDEFF* KKVLEESRSEPTRFSRGLSA c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c

2460 WGAM c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g

2520 g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c

BspHI 2580 t t c t t c g c c c a c c c c a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a a g a a g c g g g t g g g g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t

2640 a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t

2700 ori t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c

2760 ori c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a

2820 ori g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g

2880 ori c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c

2940 ori g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c

ApaLI 3000 ori g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t

3060 ori a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g

3120 ori g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c

3180 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g

3240 ori t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g

PciI 3300 a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a

3359 c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.