Addgene pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry


Map View

pBRforEco (8198 .. 8216) Amp-R (7788 .. 7807) PvuI (7608) FspI (7460) pBR322ori-F (6486 .. 6505) rbglobpA-R (6026 .. 6045) Bglob-pA-R (5907 .. 5926) BglII (5904) M13/pUC Reverse (5802 .. 5824) M13 Reverse (5789 .. 5805) M13 rev T3 (5750 .. 5770) T3 promoter BGH-rev (5537 .. 5554) WPRE-R (4975 .. 4995) PaeR7I - PspXI - XhoI (4743) AgeI (4736) mCherry-F (4438 .. 4457) SbfI (4380) mCherry-R (4170 .. 4188) SpeI (167) CMV enhancer SnaBI (508) CMV-F (687 .. 707) LNCX (733 .. 757) HindIII (763) AflII (835) Kozak sequence EcoNI (1070) PpuMI (1075) BstBI (1360) BspEI (1563) XbaI * (1582) BspQI - SapI (1818) SphI (2098) RsrII (2110) PshAI (2135) BclI * (2424) TspMI - XmaI (2917) SmaI (2919) PspOMI (3551) ApaI (3555) IRES reverse (3605 .. 3622) PmlI (3754) PaqCI (3777) DraIII (3801) IRES-F (3832 .. 3851) BmgBI (3981) pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry 8270 bp

Sequence View

60 g c c t g c c t c c a g g c c t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a c g g a c g g a g g t c c g g a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t

120 a a t c a a t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t t a g t t a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t

SpeI 180 CMV enhancer t a t t g g c t c a t g t c c a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a a t a a c c g a g t a c a g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t

240 CMV enhancer g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a

300 CMV enhancer t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a

360 CMV enhancer g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t

420 CMV enhancer t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g

480 CMV enhancer t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c

SnaBI 540 CMV enhancer CMV promoter g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c

600 CMV promoter g t t t t g g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c a a a a c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a

660 CMV promoter c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t

720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a t g t c g t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t t a c a g c a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a

HindIII 780 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a

AflII 840 t t a t c a c a g t t a a a t t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g a a t a g t g t c a a t t t a a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c

900 chimeric intron c t g c a g a a g t t g g t c g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a g a c g t c t t c a a c c a g c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t

960 chimeric intron a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g

1020 chimeric intron Kozak sequence a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g c t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c g

PpuMIEcoNI 1080 Kozak sequence MDLPDDHYLSTQTILSKDL c a c c a t g g a t c t g c c t g a t g a c c a c t a c c t g a g c a c a c a g a c c a t c c t c t c c a a g g a c c t g t g g t a c c t a g a c g g a c t a c t g g t g a t g g a c t c g t g t g t c t g g t a g g a g a g g t t c c t g g a

1140 NGSSASGGSTSAQWNQLQQL t a a c g g g a g c t c t g c c a g c g g t g g c a g c a c c t c c g c t c a g t g g a a c c a g c t g c a g c a g c t a t t g c c c t c g a g a c g g t c g c c a c c g t c g t g g a g g c g a g t c a c c t t g g t c g a c g t c g t c g a

1200 DTRYLEQLHQLYSDSFPMEL g g a c a c a c g c t a c c t g g a g c a g c t g c a c c a g c t g t a c a g c g a c a g c t t c c c c a t g g a g c t c c t g t g t g c g a t g g a c c t c g t c g a c g t g g t c g a c a t g t c g c t g t c g a a g g g g t a c c t c g a

1260 RQFLAPWIESQDWAYAASKE g c g g c a g t t c c t g g c a c c t t g g a t t g a g a g t c a a g a c t g g g c a t a t g c a g c c a g c a a a g a c g c c g t c a a g g a c c g t g g a a c c t a a c t c t c a g t t c t g a c c c g t a t a c g t c g g t c g t t t c t

1320 SHATLVFHNLLGEIDQQYSR g t c a c a t g c c a c g t t g g t g t t t c a t a a t c t c t t g g g t g a a a t t g a c c a g c a a t a t a g c c g c a g t g t a c g g t g c a a c c a c a a a g t a t t a g a g a a c c c a c t t t a a c t g g t c g t t a t a t c g g c

BstBI 1380 FLQESNVLYQHNLRRIKQFL a t t c c t g c a a g a g t c c a a t g t c c t c t a t c a g c a c a a c c t t c g a a g a a t c a a g c a g t t t c t t a a g g a c g t t c t c a g g t t a c a g g a g a t a g t c g t g t t g g a a g c t t c t t a g t t c g t c a a a g a

1440 QSRYLEKPMEIARIVARCLW g c a g a g c a g g t a t c t t g a g a a g c c a a t g g a a a t t g c c c g g a t t g t g g c c c g a t g c c t g t g c g t c t c g t c c a t a g a a c t c t t c g g t t a c c t t t a a c g g g c c t a a c a c c g g g c t a c g g a c a c

1500 EESRLLQTAATAAQQGGQAN g g a a g a g t c t c g c c t c c t c c a g a c g g c a g c c a c g g c a g c c c a g c a a g g g g g c c a g g c c a a c c t t c t c a g a g c g g a g g a g g t c t g c c g t c g g t g c c g t c g g g t c g t t c c c c c g g t c c g g t t

1560 HPTAAVVTEKQQMLEQHLQD c c a c c c a a c a g c c g c c g t a g t g a c a g a g a a g c a g c a g a t g t t g g a g c a g c a t c t t c a g g a g g t g g g t t g t c g g c g g c a t c a c t g t c t c t t c g t c g t c t a c a a c c t c g t c g t a g a a g t c c t

XbaI*BspEI 1620 VRKRVQDLEQKMKVVENLQD t g t c c g g a a g c g a g t g c a g g a t c t a g a a c a g a a a a t g a a g g t g g t g g a g a a c c t c c a g g a a c a g g c c t t c g c t c a c g t c c t a g a t c t t g t c t t t t a c t t c c a c c a c c t c t t g g a g g t c c t

1680 DFDFNYKTLKSQGDMQDLNG c g a c t t t g a t t t c a a c t a c a a a a c c c t c a a g a g c c a a g g a g a c a t g c a g g a t c t g a a t g g g c t g a a a c t a a a g t t g a t g t t t t g g g a g t t c t c g g t t c c t c t g t a c g t c c t a g a c t t a c c

1740 NNQSVTRQKMQQLEQMLTAL a a a c a a c c a g t c t g t g a c c a g a c a g a a g a t g c a g c a g c t g g a a c a g a t g c t c a c a g c c c t t t t g t t g g t c a g a c a c t g g t c t g t c t t c t a c g t c g t c g a c c t t g t c t a c g a g t g t c g g g a

1800 DQMRRSIVSELAGLLSAMEY g g a c c a g a t g c g g a g a a g c a t t g t g a g t g a g c t g g c g g g g c t c t t g t c a g c a a t g g a g t a c c t g g t c t a c g c c t c t t c g t a a c a c t c a c t c g a c c g c c c c g a g a a c a g t c g t t a c c t c a t

BspQISapI 1860 VQKTLTDEELADWKRRQQIA c g t g c a g a a g a c a c t g a c t g a t g a a g a g c t g g c t g a c t g g a a g a g g c g g c a g c a g a t c g c g c a c g t c t t c t g t g a c t g a c t a c t t c t c g a c c g a c t g a c c t t c t c c g c c g t c g t c t a g c g

1920 CIGGPPNICLDRLENWITSL g t g c a t c g g a g g c c c t c c c a a c a t c t g c c t g g a c c g t c t g g a a a a c t g g a t a a c t t c a t t c a c g t a g c c t c c g g g a g g g t t g t a g a c g g a c c t g g c a g a c c t t t t g a c c t a t t g a a g t a a

1980 AESQLQTRQQIKKLEELQQK a g c a g a a t c t c a a c t t c a g a c c c g c c a a c a a a t t a a g a a a c t g g a g g a g c t g c a g c a g a a t c g t c t t a g a g t t g a a g t c t g g g c g g t t g t t t a a t t c t t t g a c c t c c t c g a c g t c g t c t t

2040 VSYKGDPIVQHRPMLEERIV a g t g t c c t a c a a g g g c g a c c c t a t c g t g c a g c a c c g g c c c a t g c t g g a g g a g a g g a t c g t t c a c a g g a t g t t c c c g c t g g g a t a g c a c g t c g t g g c c g g g t a c g a c c t c c t c t c c t a g c a

SphI 2100 ELFRNLMKSAFVVERQPCMP g g a g c t g t t c a g a a a c t t a a t g a a g a g t g c c t t c g t g g t g g a g c g g c a g c c c t g c a t g c c c c t c g a c a a g t c t t t g a a t t a c t t c t c a c g g a a g c a c c a c c t c g c c g t c g g g a c g t a c g g

PshAIRsrII 2160 MHPDRPLVIKTGVQFTTKVR c a t g c a c c c g g a c c g g c c c t t a g t c a t c a a g a c t g g t g t c c a g t t t a c c a c g a a a g t c a g g t a c g t g g g c c t g g c c g g g a a t c a g t a g t t c t g a c c a c a g g t c a a a t g g t g c t t t c a g t c

2220 LLVKFPELNYQLKIKVCIDK g t t g c t g g t c a a a t t t c c t g a g t t g a a t t a t c a g c t t a a a a t t a a a g t g t g c a t t g a t a a c a a c g a c c a g t t t a a a g g a c t c a a c t t a a t a g t c g a a t t t t a a t t t c a c a c g t a a c t a t t

2280 DSGDVAALRGSRKFNILGTN a g a c t c t g g g g a t g t t g c t g c c c t c a g a g g g t c t c g g a a a t t t a a c a t t c t g g g c a c g a a t c t g a g a c c c c t a c a a c g a c g g g a g t c t c c c a g a g c c t t t a a a t t g t a a g a c c c g t g c t t

2340 TKVMNMEESNNGSLSAEFKH c a c a a a a g t g a t g a a c a t g g a g g a g t c t a a c a a c g g c a g c c t g t c t g c a g a g t t c a a g c a g t g t t t t c a c t a c t t g t a c c t c c t c a g a t t g t t g c c g t c g g a c a g a c g t c t c a a g t t c g t

2400 LTLREQRCGNGGRANCDASL c c t g a c c c t t a g g g a g c a g a g a t g t g g g a a t g g a g g c c g t g c c a a t t g t g a t g c c t c c t t g g a c t g g g a a t c c c t c g t c t c t a c a c c c t t a c c t c c g g c a c g g t t a a c a c t a c g g a g g a a

BclI* 2460 IVTEELHLITFETEVYHQGL g a t c g t g a c t g a g g a g c t g c a c c t g a t c a c c t t c g a g a c t g a g g t g t a c c a c c a a g g c c t c t a g c a c t g a c t c c t c g a c g t g g a c t a g t g g a a g c t c t g a c t c c a c a t g g t g g t t c c g g a

2520 KIDLETHSLPVVVISNICQM c a a g a t t g a c c t a g a g a c c c a c t c c t t g c c a g t t g t g g t g a t c t c c a a c a t c t g t c a g a t g t t c t a a c t g g a t c t c t g g g t g a g g a a c g g t c a a c a c c a c t a g a g g t t g t a g a c a g t c t a

2580 PNAWASILWYNMLTNNPKNV g c c a a a t g c t t g g g c a t c a a t c c t g t g g t a t a a c a t g c t g a c c a a t a a c c c c a a g a a c g t c g g t t t a c g a a c c c g t a g t t a g g a c a c c a t a t t g t a c g a c t g g t t a t t g g g g t t c t t g c a

2640 NFFTKPPIGTWDQVAEVLSW g a a c t t c t t c a c t a a g c c g c c a a t t g g a a c c t g g g a c c a a g t g g c c g a g g t g c t c a g c t g c t t g a a g a a g t g a t t c g g c g g t t a a c c t t g g a c c c t g g t t c a c c g g c t c c a c g a g t c g a c

2700 QFSSTTKRGLSIEQLTTLAE g c a g t t c t c g t c c a c c a c c a a g c g g g g g c t g a g c a t c g a g c a g c t g a c a a c g c t g g c t g a c g t c a a g a g c a g g t g g t g g t t c g c c c c c g a c t c g t a g c t c g t c g a c t g t t g c g a c c g a c t

2760 KLLGPGVNYSGCQITWAKFC g a a g c t c c t a g g g c c t g g t g t g a a c t a c t c a g g g t g t c a g a t c a c a t g g g c t a a a t t c t g c t t c g a g g a t c c c g g a c c a c a c t t g a t g a g t c c c a c a g t c t a g t g t a c c c g a t t t a a g a c

2820 KENMAGKGFSFWVWLDNIID c a a a g a a a a c a t g g c t g g c a a g g g c t t c t c c t t c t g g g t c t g g c t a g a c a a t a t c a t c g a g t t t c t t t t g t a c c g a c c g t t c c c g a a g a g g a a g a c c c a g a c c g a t c t g t t a t a g t a g c t

2880 LVKKYILALWNEGYIMGFIS c c t t g t g a a a a a g t a t a t c t t g g c c c t t t g g a a t g a a g g g t a c a t c a t g g g t t t c a t c a g g g a a c a c t t t t t c a t a t a g a a c c g g g a a a c c t t a c t t c c c a t g t a g t a c c c a a a g t a g t c

TspMIXmaISmaI 2940 KERERAILSTKPPGTFLLQF c a a g g a g c g g g a g c g g g c c a t c c t a a g c a c a a a g c c c c c g g g c a c c t t c c t a c t g c a g t t g t t c c t c g c c c t c g c c c g g t a g g a t t c g t g t t t c g g g g g c c c g t g g a a g g a t g a c g t c a a

3000 SESSKEGGVTFTWVEKDISG c a g c g a g a g c a g c a a a g a a g g a g g g g t c a c t t t c a c t t g g g t g g a a a a g g a c a t c a g t g g g t c g c t c t c g t c g t t t c t t c c t c c c c a g t g a a a g t g a a c c c a c c t t t t c c t g t a g t c a c c

3060 KTQIQSVEPYTKQQLNNMSF c a a g a c c c a g a t c c a g t c t g t a g a g c c a t a c a c c a a g c a g c a g c t g a a c a a c a t g t c a t t g t t c t g g g t c t a g g t c a g a c a t c t c g g t a t g t g g t t c g t c g t c g a c t t g t t g t a c a g t a a

3120 AEIIMGYKIMDATNILVSPL t g c t g a a a t c a t c a t g g g c t a t a a g a t c a t g g a t g c g a c c a a c a t c c t g g t g t c t c c a c t a c g a c t t t a g t a g t a c c c g a t a t t c t a g t a c c t a c g c t g g t t g t a g g a c c a c a g a g g t g a

3180 VYLYPDIPKEEAFGKYCRPE t g t c t a c c t c t a c c c c g a c a t t c c c a a g g a g g a g g c a t t c g g a a a g t a c t g t a g g c c c g a a c a g a t g g a g a t g g g g c t g t a a g g g t t c c t c c t c c g t a a g c c t t t c a t g a c a t c c g g g c t

3240 SQEHPEADPGSAAPYLKTKF g a g c c a g g a g c a c c c c g a a g c c g a c c c a g g t a g t g c t g c c c c g t a c c t g a a g a c c a a g t t c t c g g t c c t c g t g g g g c t t c g g c t g g g t c c a t c a c g a c g g g g c a t g g a c t t c t g g t t c a a

3300 ICVTPTTCSNTIDLPMSPRT c a t c t g t g t g a c a c c a a c g a c c t g c a g c a a t a c c a t t g a c c t g c c g a t g t c c c c c c g c a c g t a g a c a c a c t g t g g t t g c t g g a c g t c g t t a t g g t a a c t g g a c g g c t a c a g g g g g g c g t g

3360 LDSLMQFGNNGEGAEPSAGG t t t a g a t t c a t t g a t g c a g t t t g g a a a t a a c g g t g a a g g t g c t g a g c c c t c a g c a g g a g g a a a t c t a a g t a a c t a c g t c a a a c c t t t a t t g c c a c t t c c a c g a c t c g g g a g t c g t c c t c c

3420 QFESLTFDMDLTSECATSPM g c a g t t t g a g t c g c t c a c g t t t g a c a t g g a t c t g a c c t c g g a g t g t g c t a c c t c c c c c a t c g t c a a a c t c a g c g a g t g c a a a c t g t a c c t a g a c t g g a g c c t c a c a c g a t g g a g g g g g t a

3480 IRES2 * g t a a g a a t t c a g a t c c g c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c c a t t c t t a a g t c t a g g c g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g

3540 IRES2 t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a

PspOMIApaI 3600 IRES2 g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a

3660 IRES2 G A G A G C G G T T T C C T T A C G IRES reverse t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c

3720 IRES2 g t a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c a t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t

PaqCIPmlI 3780 IRES2 c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c

DraIII 3840 IRES2 T G G C T C T C C IRES-F g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g

3900 IRES2 T C A A G C G T A T T IRES-F t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a

3960 IRES2 g a t c t g g g g c c t c g g t a c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a c t a g a c c c c g g a g c c a t g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t

BmgBI 4020 ATG IRES2 MAT g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g

4080 1 5 10 15 MVSKGEEDNMAIIKEFMRF IRES2 mCherry TMVSKGEEDNMAIIKEFMRF a a c c a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t t t g g t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a

4140 20 25 30 35 KVHMEGSVNGHEFEIEGEGE mCherry KVHMEGSVNGHEFEIEGEGE c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t

4200 40 45 50 55 GRPYEGTQTAKLKVTKGGPL mCherry G G T T C G A C T T C C A C T G G T T mCherry-R GRPYEGTQTAKLKVTKGGPL g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a

4260 60 65 70 75 PFAWDILSPQFMYGSKAYVK mCherry PFAWDILSPQFMYGSKAYVK g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t

4320 80 85 90 95 HPADIPDYLKLSFPEGFKWE mCherry HPADIPDYLKLSFPEGFKWE g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t

SbfI 4380 100 105 110 115 RVMNFEDGGVVTVTQDSSLQ mCherry RVMNFEDGGVVTVTQDSSLQ g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t

4440 120 125 130 135 DGEFIYKVKLRGTNFPSDGP mCherry C C C mCherry-F DGEFIYKVKLRGTNFPSDGP g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g

4500 140 145 150 155 VMQKKTMGWEASSERMYPED mCherry C G T A A T G C A G A A G A A G A mCherry-F VMQKKTMGWEASSERMYPED c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t

4560 160 165 170 175 GALKGEIKQRLKLKDGGHYD mCherry GALKGEIKQRLKLKDGGHYD c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t

4620 180 185 190 195 AEVKTTYKAKKPVQLPGAYN mCherry AEVKTTYKAKKPVQLPGAYN c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t

4680 200 205 210 215 VNIKLDITSHNEDYTIVEQY mCherry VNIKLDITSHNEDYTIVEQY c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t

AgeI 4740 220 225 230 235 ERAEGRHSTGGMDELYK* mCherry ERAEGRHSTGGMDELYK* c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t a a a c c g g g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a t t t g g c c

PaeR7IPspXIXhoI 4800 chimeric intron t g c t c g a g g t a c c g a t g a a t a g c t a a g g t c g a g g c c g c a g g t a a g t a t c a a g g t t a c a a g a c g a g c t c c a t g g c t a c t t a t c g a t t c c a g c t c c g g c g t c c a t t c a t a g t t c c a a t g t t c

4860 chimeric intron a c a g g t t t a a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t t g t c c a a a t t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a

4920 chimeric intron c t g a t a g g c a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c g a g a c t a t c c g t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g c t

4980 WPRE A C A A C G WPRE-R c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g

5040 WPRE (in frame with Factor Xa site) *HRQIMSNSGT A G G A A A A T G C G A T A C WPRE-R MPLYHAIASR t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c

5100 WPRE (in frame with Factor Xa site) HSENEGGQIFGPQQRKILLQ MAFIFSSLYKSWLLSLYEEL t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a

5160 WPRE (in frame with Factor Xa site) PGNDPLTAHHASHKSVCGGS WPVVRQRGVVCTVFADATPT g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g

5220 WPRE (in frame with Factor Xa site) TPANGGGTLEKGPSESEGER GWGIATTCQLLSGTFAFPLP t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g

5280 WPRE (in frame with Factor Xa site) NGRRFEDGGAKGAAPCPSPQ IATAELIAACLARCWTGARL t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a

5340 WPRE (in frame with Factor Xa site) QASVIGHHQRPLQRGKWPQE LGTDNSVVLSGKLTSFPWLL g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a

5400 WPRE (in frame with Factor Xa site) GTNGGPNQAPRGEAVDR RGE Factor Xa site ACVATWILRGTSFCYVPSAL c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a

5460 WPRE 1 I Factor Xa site NPADLPSRGLLPALRPLPRL c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a

5520 WPRE RLRPQTSRISLWAASPPGIR t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g g a a t t c g a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c c t t a a g c

5580 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev ARYDQPRLCLLVASHLLFAP a g c t c g g t a c g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c g a g c c a t g c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g

5640 bGH poly(A) signal PPCLP* t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a

5700 bGH poly(A) signal g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c

5760 T3 promoter G G A A A T C A C T C T3 c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c c c a g c t t t t g t t c c c t t t a g t g a g g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g g g t c g a a a a c a a g g g a a a t c a c t c

5820 T3 promoter M13 rev C C A A T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G M13/pUC Reverse g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g

5880 G C G A M13/pUC Reverse c g c t a a t t c a c t c c t c a g g t g c a g g c t g c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c g a t t a a g t g a g g a g t c c a c g t c c g a c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a

BglII 5940 A G A A A A A G G G A G A C G G T T T T Bglob-pA-R g c c c t g g c t c a c a a a t a c c a c t g a g a t c t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c c g g g a c c g a g t g t t t a t g g t g a c t c t a g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g

6000 β- globin poly(A) signal a t g a a g c c c c t t g a g c a t c t g a c t t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a t a c t t c g g g g a a c t c g t a g a c t g a a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t

6060 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t

6120 a a a c a t c a g a a t g a g t a t t t g g t t t a g a g t t t g g c a a c a t a t g c c c a t a t g c t g g c t g c c t t t g t a g t c t t a c t c a t a a a c c a a a t c t c a a a c c g t t g t a t a c g g g t a t a c g a c c g a c g g

6180 a t g a a c a a a g g t t g g c t a t a a a g a g g t c a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t a c t t g t t t c c a a c c g a t a t t t c t c c a g t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a

6240 t c c t t a t t c c a t a g a a a a g c c t t g a c t t g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g a g g a a t a a g g t a t c t t t t c g g a a c t g a a c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c

6300 t t a t t t t t t t c t t t a a c a t c c c t a a a a t t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c a a t a a a a a a a g a a a t t g t a g g g a t t t t a a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g

6360 c t c c t c t c c t g a c t a c t c c c a g t c a t a g c t g t c c c t c t t c t c t t a t g g a g a t c g g c c g g c g a g g a g a g g a c t g a t g a g g g t c a g t a t c g a c a g g g a g a a g a g a a t a c c t c t a g c c g g c c g

6420 ori c g c c g g c g t c g a c g c g c g c g g c c g c g g c g c g c c g t t g c t g g c g t t t t t c c a t a g g c t c c g g c g g c c g c a g c t g c g c g c g c c g g c g c c g c g c g g c a a c g a c c g c a a a a a g g t a t c c g a g g c

6480 ori c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c

6540 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g

6600 ori c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t

6660 ori t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a

6720 ori g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g

6780 ori c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c

6840 ori a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g

6900 ori t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a

6960 ori t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t

7020 ori g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c

7080 g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t

7140 a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a

7200 285 280 *WHKILSAGIEA AmpR *WHKILSAGIEA a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g

7260 275 270 265 260 IQRNREDMTAQSGTTYIVVI AmpR IQRNREDMTAQSGTTYIVVI g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a

7320 255 250 245 240 RSPKGDPGLAAIIGRSGREG AmpR RSPKGDPGLAAIIGRSGREG a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g

7380 235 230 225 220 AGSKDAIFWGAPLASRLLPG AmpR AGSKDAIFWGAPLASRLLPG g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g

7440 215 210 205 200 AVKDAEMWDILQQRSALTLL AmpR AVKDAEMWDILQQRSALTLL t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c

FspI 7500 195 190 185 180 EGTLLKRLTTAMAVPMTTDR AmpR EGTLLKRLTTAMAVPMTTDR t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c

7560 175 170 165 160 EDNPIAENLEPEWRDLRTVH AmpR EDNPIAENLEPEWRDLRTVH c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c

PvuI 7620 155 150 145 140 DGMNHLFATLEKPGGITTLL AmpR DGMNHLFATLEKPGGITTLL a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c

7680 135 130 125 120 LNAATNDSMTIAASCLERVT AmpR LNAATNDSMTIAASCLERVT t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a

7740 115 110 105 100 MGDTLHKETVPSYEVLDNQS AmpR MGDTLHKETVPSYEVLDNQS c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t

7800 95 90 85 80 YHIRRGLQEQGADIRSLVAG AmpR A T A A T A C C G C G C C Amp-R YHIRRGLQEQGADIRSLVAG a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g

7860 75 70 65 60 CLLVKFTSMMPFREEPRFSE AmpR A C A T A G C Amp-R CLLVKFTSMMPFREEPRFSE a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g

7920 55 50 45 40 LIKGSNLDLEIYGVRAGLQD AmpR LIKGSNLDLEIYGVRAGLQD a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g

7980 35 30 25 EADKVKVLTEPH 20 signal sequence AFVPLCFA AmpR EADKVKVLTEPHAFVPLCFA t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g

8040 15 10 5 1 signal sequence AFFPILAVRFHQISM AmpR AmpR promoter AFFPILAVRFHQISM c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t

8100 AmpR promoter a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a

8160 AmpR promoter t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a

8220 A A T A G G C G T A T C A C G A G G C pBRforEco c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a

8270 t c g t c t a c g c g c g c g c c g g c g g c g c g c c g t c g a c g g c c g g c c g c g g c c g c a g c a g a t g c g c g c g c g g c c g c c g c g c g g c a g c t g c c g g c c g g c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.