Addgene pRDAX-108 N-Tag GFP[2] L10 FRB EBPF2 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRDAX-108 N-Tag GFP[2] L10 FRB EBPF2


Map View

pBRforEco (6188 .. 6206) BsrBI (6069) Amp-R (5778 .. 5797) ScaI (5708) PvuI (5598) FspI (5450) AhdI (5228) HaeII (4583) pBR322ori-F (4476 .. 4495) rbglobpA-R (4016 .. 4035) Bglob-pA-R (3897 .. 3916) BglII (3894) Bsu36I (3824) M13/pUC Reverse (3792 .. 3814) M13 Reverse (3779 .. 3795) M13 rev T3 (3740 .. 3760) BGH-rev (3527 .. 3544) SpeI (167) CMV enhancer SnaBI (508) CMV-F (687 .. 707) LNCX (733 .. 757) HindIII (763) AflII (835) PstI (845) Kozak sequence PvuII (1070) EcoNI (1160) SphI (1232) AvrII (1570) IRES reverse (1586 .. 1603) PmlI (1735) PaqCI (1758) IRES-F (1813 .. 1832) BmgBI (1962) EGFP-N (2051 .. 2072) BlpI (2627) AgeI (2726) WPRE-R (2965 .. 2985) pRDAX-108 N-Tag GFP[2] L10 FRB EBPF2 6260 bp

Sequence View

60 g c c t g c c t c c a g g c c t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a c g g a c g g a g g t c c g g a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t

120 a a t c a a t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t t a g t t a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t

SpeI 180 CMV enhancer t a t t g g c t c a t g t c c a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a a t a a c c g a g t a c a g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t

240 CMV enhancer g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a

300 CMV enhancer t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a

360 CMV enhancer g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t

420 CMV enhancer t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g

480 CMV enhancer t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c

SnaBI 540 CMV enhancer CMV promoter g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c

600 CMV promoter g t t t t g g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c a a a a c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a

660 CMV promoter c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t

720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a t g t c g t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t t a c a g c a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a

HindIII 780 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a

AflII 840 t t a t c a c a g t t a a a t t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g a a t a g t g t c a a t t t a a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c

PstI 900 chimeric intron c t g c a g a a g t t g g t c g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a g a c g t c t t c a a c c a g c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t

960 chimeric intron a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g

1020 chimeric intron Kozak sequence a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g c t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c g

PvuII 1080 Kozak sequence c a c c a t g g a a a a g a g g g a c c a c a t g g t g c t g c t c g a g t a c g t c a c a g c a g c t g g c a t c a c g t g g t a c c t t t t c t c c c t g g t g t a c c a c g a c g a g c t c a t g c a g t g t c g t c g a c c g t a g t g

1140 2025 ILWHE FRB c g a t g c c a g c g g g a g c t c t g c c a g c g g t g g c a g c a c c t c c g t g g c c a t c c t c t g g c a t g a g c t a c g g t c g c c c t c g a g a c g g t c g c c a c c g t c g t g g a g g c a c c g g t a g g a g a c c g t a c t

EcoNI 1200 2030 2035 2040 2045 MWHEGLEEASRLYFGERNVK FRB g a t g t g g c a t g a a g g c c t g g a a g a g g c a t c t c g t t t g t a c t t t g g g g a a a g g a a c g t g a a c t a c a c c g t a c t t c c g g a c c t t c t c c g t a g a g c a a a c a t g a a a c c c c t t t c c t t g c a c t t

SphI 1260 2050 2055 2060 2065 GMFEVLEPLHAMMERGPQTL FRB a g g c a t g t t t g a g g t g c t g g a g c c c t t g c a t g c t a t g a t g g a a c g g g g c c c c c a g a c t c t t c c g t a c a a a c t c c a c g a c c t c g g g a a c g t a c g a t a c t a c c t t g c c c c g g g g g t c t g a g a

1320 2070 2075 2080 2085 KETSFNQAYGRDLMEAQEWC FRB g a a g g a a a c a t c c t t t a a t c a g g c c t a t g g t c g a g a t t t a a t g g a g g c c c a a g a g t g g t g c t t c c t t t g t a g g a a a t t a g t c c g g a t a c c a g c t c t a a a t t a c c t c c g g g t t c t c a c c a c

1380 2090 2095 2100 2105 RKYMKSGNVKDLTQAWDLYY FRB c a g g a a g t a c a t g a a a t c a g g g a a t g t c a a g g a c c t c a c c c a a g c c t g g g a c c t c t a t t a g t c c t t c a t g t a c t t t a g t c c c t t a c a g t t c c t g g a g t g g g t t c g g a c c c t g g a g a t a a t

1440 2110 HVFRRIS * FRB IRES2 t c a t g t g t t c c g a c g a a t c t c a t a a g a a t t c a g a t c c g c c c c t c t c c c t c c c c c c c c c c t a g t a c a c a a g g c t g c t t a g a g t a t t c t t a a g t c t a g g c g g g g a g a g g g a g g g g g g g g g g a

1500 IRES2 a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a

1560 IRES2 t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c

AvrII 1620 IRES2 G A G A G C G G T T T C C T T A C G IRES reverse a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g

1680 IRES2 g t g a a g g a a g c a g t t c c t c t g g t a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t c a c t t c c t t c g t c a a g g a g a c c a t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a

PmlI 1740 IRES2 t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t

PaqCI 1800 IRES2 t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c

1860 IRES2 T G G C T C T C C T C A A G C G T A T T IRES-F g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c

1920 IRES2 g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t a c a c a t g c t t t a c a t g t g t t t a g c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a t g t g t a c g a a a t g t a c a c a a a t c

BmgBI 1980 IRES2 t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t

2040 ATG 1 M 1a V 5 10 SKGEELFTGV IRES2 EBFP2 MATTMVSKGEELFTGV a c a c g a t g a t a a t a t g g c c a c a a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t t g t g c t a c t a t t a t a c c g g t g t t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a

2100 15 20 25 30 VPILVELDGDVNGHKFSVRG EBFP2 G A C C A G C T C G A C C T G C C G C T G C EGFP-N VPILVELDGDVNGHKFSVRG g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g a g g g g c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c t c c c c

2160 35 40 45 50 EGEGDATNGKLTLKFICTTG EBFP2 EGEGDATNGKLTLKFICTTG c g a g g g c g a g g g c g a t g c c a c c a a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g g c t c c c g c t c c c g c t a c g g t g g t t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c

2220 55 60 65 70 KLPVPWPTLVTTLSHGVQCF EBFP2 KLPVPWPTLVTTLSHGVQCF c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a g c c a c g g c g t g c a g t g c t t g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t c g g t g c c g c a c g t c a c g a a

2280 75 80 85 90 ARYPDHMKQHDFFKSAMPEG EBFP2 ARYPDHMKQHDFFKSAMPEG c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c

2340 95 100 105 110 YVQERTIFFKDDGTYKTRAE EBFP2 YVQERTIFFKDDGTYKTRAE c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a c c t a c a a g a c c c g c g c c g a g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t g g a t g t t c t g g g c g c g g c t

2400 115 120 125 130 VKFEGDTLVNRIELKGVDFK EBFP2 VKFEGDTLVNRIELKGVDFK g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c g t c g a c t t c a a c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g c a g c t g a a g t t

2460 135 140 145 150 EDGNILGHKLEYNFNSHNIY EBFP2 EDGNILGHKLEYNFNSHNIY g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t t c a a c a g c c a c a a c a t c t a c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a a g t t g t c g g t g t t g t a g a t

2520 155 160 165 170 IMAVKQKNGIKVNFKIRHNV EBFP2 IMAVKQKNGIKVNFKIRHNV t a t c a t g g c c g t c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c g t a t a g t a c c g g c a g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g c a

2580 175 180 185 190 EDGSVQLADHYQQNTPIGDG EBFP2 EDGSVQLADHYQQNTPIGDG g g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c

BlpI 2640 195 200 205 210 PVLLPDSHYLSTQSVLSKDP EBFP2 PVLLPDSHYLSTQSVLSKDP c c c c g t g c t g c t g c c c g a c a g c c a c t a c c t g a g c a c c c a g t c c g t g c t g a g c a a a g a c c c g g g g c a c g a c g a c g g g c t g t c g g t g a t g g a c t c g t g g g t c a g g c a c g a c t c g t t t c t g g g

2700 215 220 225 230 NEKRDHMVLLEFRTAAGITL EBFP2 NEKRDHMVLLEFRTAAGITL c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c c g c a c c g c c g c c g g g a t c a c t c t g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g g c g t g g c g g c g g c c c t a g t g a g a

AgeI 2760 235 GMDELYK* EBFP2 GMDELYK* c g g c a t g g a c g a g c t g t a c a a g t a a a c c g g t g c t c g a g g t a c c g a t g a a t a g c t a a g g t a g c c g t a c c t g c t c g a c a t g t t c a t t t g g c c a c g a g c t c c a t g g c t a c t t a t c g a t t c c a t

2820 chimeric intron g a g g c c g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a a c t g g g c t c c g g c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t t g a c c c

2880 chimeric intron c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c a t g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g a c t g t a

2940 chimeric intron WPRE c c a c t t t g c c t t t c t c t c c a c a g g t g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a g g t g a a a c g g a a a g a g a g g t g t c c a c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t

3000 WPRE * A C A A C G A G G A A A A T G C G A T A C WPRE-R a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a

3060 WPRE (in frame with Factor Xa site) HRQIMSNSGTHSENEGGQIF MPLYHAIASRMAFIFSSLYK a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t

3120 WPRE (in frame with Factor Xa site) GPQQRKILLQPGNDPLTAHH SWLLSLYEELWPVVRQRGVV a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a

3180 WPRE (in frame with Factor Xa site) ASHKSVCGGSTPANGGGTLE CTVFADATPTGWGIATTCQL g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a

3240 WPRE (in frame with Factor Xa site) KGPSESEGERNGRRFEDGGA LSGTFAFPLPIATAELIAAC c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c

3300 WPRE (in frame with Factor Xa site) KGAAPCPSPQQASVIGHHQR LARCWTGARLLGTDNSVVLS c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g

3360 WPRE (in frame with Factor Xa site) PLQRGKWPQEGTNGGPNQAP GKLTSFPWLLACVATWILRG g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c

3420 WPRE (in frame with Factor Xa site) RGEAVDR 1 RGEI Factor Xa site TSFCYVPSALNPADLPSRGL g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a

3480 WPRE LPALRPLPRLRLRPQTSRIS g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g

3540 WPRE bGH poly(A) signal G G A G C T G A C A C G G A BGH-rev LWAASPPGIRARYDQPRLCL c c t t t g g g c c g c c t c c c c g c c t g g a a t t c g a g c t c g g t a c g a t c a g c c t c g a c t g t g c c t g g a a a c c c g g c g g a g g g g c g g a c c t t a a g c t c g a g c c a t g c t a g t c g g a g c t g a c a c g g a

3600 bGH poly(A) signal A G A T BGH-rev LVASHLLFAPPPCLP* t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a

3660 bGH poly(A) signal g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c

3720 t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g

3780 T3 promoter M13 rev G G A A A T C A C T C C C A A T T A A C G T3 C A M13 Reverse a a t a g c c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t t t a t c g g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a

Bsu36I 3840 M13 rev G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t a a t t c a c t c c t c a g g t g c a g g c t g c c g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a t t a a g t g a g g a g t c c a c g t c c g a c g g

BglII 3900 A G A A Bglob-pA-R t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a g a t c t t a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t c t a g a a

3960 A A A G G G A G A C G G T T T T Bglob-pA-R t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t t c t g g c a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a a g a c c g

4020 β- globin poly(A) signal G T G A G rbglobpA-R t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g

4080 C C T T C C T G T A T A C C C rbglobpA-R g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t t a g a g t c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a a t c t c a

4140 t t g g c a a c a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a g g t c a t a a c c g t t g t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t c c a g t a

4200 c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g a c t t g a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c t g a a c t

4260 g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a a a a t t t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t t t t a a a

4320 t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c a t a g c t a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g t a t c g a

4380 g t c c c t c t t c t c t t a t g g a g a t c g g c c g g c c g c c g g c g t c g a c g c g c g c g g c c g c g g c g c c a g g g a g a a g a g a a t a c c t c t a g c c g g c c g g c g g c c g c a g c t g c g c g c g c c g g c g c c g c g

4440 ori g c c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c g g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c

4500 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c

4560 ori a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a

HaeII 4620 ori t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a

4680 ori g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c

4740 ori c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a

4800 ori g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a

4860 ori c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a

4920 ori g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g

4980 ori c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g

5040 ori t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c

5100 t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t

5160 *W AmpR *W a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t

5220 285 280 275 270 HKILSAGIEAIQRNREDMTA AmpR HKILSAGIEAIQRNREDMTA a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g

AhdI 5280 265 260 255 250 QSGTTYIVVIRSPKGDPGLA AmpR QSGTTYIVVIRSPKGDPGLA c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g

5340 245 240 235 230 AIIGRSGREGAGSKDAIFWG AmpR AIIGRSGREGAGSKDAIFWG t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g

5400 225 220 215 210 APLASRLLPGAVKDAEMWDI AmpR APLASRLLPGAVKDAEMWDI a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a

FspI 5460 205 200 195 190 LQQRSALTLLEGTLLKRLTT AmpR LQQRSALTLLEGTLLKRLTT t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a

5520 185 180 175 170 AMAVPMTTDREDNPIAENLE AmpR AMAVPMTTDREDNPIAENLE t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g

5580 165 160 155 150 PEWRDLRTVHDGMNHLFATL AmpR PEWRDLRTVHDGMNHLFATL c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c

PvuI 5640 145 140 135 130 EKPGGITTLLLNAATNDSMT AmpR EKPGGITTLLLNAATNDSMT c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a

5700 125 120 115 110 IAASCLERVTMGDTLHKETV AmpR IAASCLERVTMGDTLHKETV t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g

ScaI 5760 105 100 95 90 PSYEVLDNQSYHIRRGLQEQ AmpR PSYEVLDNQSYHIRRGLQEQ t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c

5820 85 80 75 70 GADIRSLVAGCLLVKFTSMM AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GADIRSLVAGCLLVKFTSMM c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a

5880 65 60 55 50 PFREEPRFSELIKGSNLDLE AmpR PFREEPRFSELIKGSNLDLE t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g

5940 45 40 35 30 IYGVRAGLQDEADKVKVLTE AmpR IYGVRAGLQDEADKVKVLTE g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g

6000 25 PH 20 15 10 signal sequence AFVPLCFAAFFPILAVRF AmpR PHAFVPLCFAAFFPILAVRF t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t

6060 5 1 signal sequence HQISM AmpR AmpR promoter HQISM a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c

BsrBI 6120 AmpR promoter t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c

6180 c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g

6240 A A T A G G C G T A T C A C G A G G C pBRforEco c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t a c g c g c g c g c c g g c g g c g c g c c g t g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a t g c g c g c g c g g c c g c c g c g c g g c a

6260 c g a c g g c c g g c c g c g g c c g c g c t g c c g g c c g g c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.