Addgene pRDAX-463 C-Tag UnaG[2] L10 FKBP mCherry - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRDAX-463 C-Tag UnaG[2] L10 FKBP mCherry


Map View

pBRforEco (6320 .. 6338) Amp-R (5910 .. 5929) ScaI (5840) PvuI (5730) FspI (5582) pBR322ori-F (4608 .. 4627) rbglobpA-R (4148 .. 4167) Bglob-pA-R (4029 .. 4048) BglII (4026) Bsu36I (3956) M13/pUC Reverse (3924 .. 3946) M13 Reverse (3911 .. 3927) M13 rev T3 (3872 .. 3892) BGH-rev (3659 .. 3676) PfoI (3317) SpeI (167) SnaBI (508) CMV-F (687 .. 707) LNCX (733 .. 757) HindIII (763) AflII (835) BsmBI - Esp3I (1050) TspMI - XmaI (1143) SmaI (1145) PspOMI (1673) ApaI (1677) AvrII (1711) IRES reverse (1727 .. 1744) PmlI (1876) DraIII (1923) IRES-F (1954 .. 1973) BmgBI (2103) mCherry-R (2292 .. 2310) SbfI (2502) mCherry-F (2560 .. 2579) BbvCI (2685) PvuII (2725) AgeI (2858) PaeR7I - PspXI - XhoI (2865) WPRE-R (3097 .. 3117) pRDAX-463 C-Tag UnaG[2] L10 FKBP mCherry 6392 bp

Sequence View

60 g c c t g c c t c c a g g c c t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a c g g a c g g a g g t c c g g a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t

120 a a t c a a t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t t a g t t a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t

SpeI 180 CMV enhancer t a t t g g c t c a t g t c c a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a a t a a c c g a g t a c a g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t

240 CMV enhancer g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a

300 CMV enhancer t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a

360 CMV enhancer g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t

420 CMV enhancer t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g

480 CMV enhancer t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c

SnaBI 540 CMV enhancer CMV promoter g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c

600 CMV promoter g t t t t g g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c a a a a c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a

660 CMV promoter c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t

720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a t g t c g t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t t a c a g c a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a

HindIII 780 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a

AflII 840 t t a t c a c a g t t a a a t t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g a a t a g t g t c a a t t t a a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c

900 chimeric intron c t g c a g a a g t t g g t c g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a g a c g t c t t c a a c c a g c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t

960 chimeric intron a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g

1020 chimeric intron Kozak sequence a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g c t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c g

BsmBIEsp3I 1080 M 1 5 10 15 GVQVETISPGDGRTFPKR FKBP Kozak sequence MGVQVETISPGDGRTFPKR c a c c a t g g g a g t g c a g g t g g a a a c c a t c t c c c c a g g a g a c g g g c g c a c c t t c c c c a a g c g g t g g t a c c c t c a c g t c c a c c t t t g g t a g a g g g g t c c t c t g c c c g c g t g g a a g g g g t t c g c

1140 20 25 30 35 GQTCVVHYTGMLEDGKKFDS FKBP GQTCVVHYTGMLEDGKKFDS c g g c c a g a c c t g c g t g g t g c a c t a c a c c g g g a t g c t t g a a g a t g g a a a g a a a t t t g a t t c g c c g g t c t g g a c g c a c c a c g t g a t g t g g c c c t a c g a a c t t c t a c c t t t c t t t a a a c t a a g

TspMIXmaISmaI 1200 40 45 50 55 SRDRNKPFKFMLGKQEVIRG FKBP SRDRNKPFKFMLGKQEVIRG c t c c c g g g a c a g a a a c a a g c c c t t t a a g t t t a t g c t a g g c a a g c a g g a g g t g a t c c g a g g g a g g g c c c t g t c t t t g t t c g g g a a a t t c a a a t a c g a t c c g t t c g t c c t c c a c t a g g c t c c

1260 60 65 70 75 WEEGVAQMSVGQRAKLTISP FKBP WEEGVAQMSVGQRAKLTISP c t g g g a a g a a g g g g t t g c c c a g a t g a g t g t g g g t c a g a g a g c c a a a c t g a c t a t a t c t c c g a c c c t t c t t c c c c a a c g g g t c t a c t c a c a c c c a g t c t c t c g g t t t g a c t g a t a t a g a g g

1320 80 85 90 95 DYAYGATGHPGIIPPHATLV FKBP DYAYGATGHPGIIPPHATLV a g a t t a t g c c t a t g g t g c c a c t g g g c a c c c a g g c a t c a t c c c a c c a c a t g c c a c t c t c g t t c t a a t a c g g a t a c c a c g g t g a c c c g t g g g t c c g t a g t a g g g t g g t g t a c g g t g a g a g c a

1380 100 105 FDVELLKLE (in frame with FKBP) GSSASGGSTSG FKBP FDVELLKLEGSSASGGSTSG c t t c g a t g t g g a g c t t c t a a a a c t g g a a g g g a g c t c t g c c a g c g g t g g c a g c a c c a g t g g g a a g c t a c a c c t c g a a g a t t t t g a c c t t c c c t c g a g a c g g t c g c c a c c g t c g t g g t c a c c

1440 (in frame with FKBP) VKSVVNLVGEKLVYVQKWDG VKSVVNLVGEKLVYVQKWDG g g t g a a g t c t g t g g t g a a t c t g g t a g g c g a g a a a c t c g t g t a c g t c c a g a a g t g g g a t g g c c a c t t c a g a c a c c a c t t a g a c c a t c c g c t c t t t g a g c a c a t g c a g g t c t t c a c c c t a c c

1500 (in frame with FKBP) KETTYVREIKDGKLVVTLTM KETTYVREIKDGKLVVTLTM g a a g g a a a c c a c g t a t g t g c g a g a g a t c a a g g a c g g g a a g t t g g t t g t c a c c c t g a c c a t c t t c c t t t g g t g c a t a c a c g c t c t c t a g t t c c t g c c c t t c a a c c a a c a g t g g g a c t g g t a

1560 (in frame with FKBP) GDVVAVRSYRRATE* IRES2 GDVVAVRSYRRATE* g g g c g a t g t g g t t g c t g t g c g c t c a t a t c g g a g a g c c a c t g a a t a a g a a t t c a g a t c c g c c c c g c t a c a c c a a c g a c a c g c g a g t a t a g c c t c t c g g t g a c t t a t t c t t a a g t c t a g g c g

1620 IRES2 c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a

PspOMIApaI 1680 IRES2 g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c

AvrII 1740 IRES2 G A G A G C G G T T T C C T IRES reverse a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t

1800 IRES2 T A C G IRES reverse a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g t a g c t t c t t g a a g a c a a t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c a t c g a a g a a c t t c t g t t

1860 IRES2 a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g

PmlI 1920 IRES2 t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g

DraIII 1980 IRES2 T G G C T C T C C T C A A G C G T A T T IRES-F g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c

2040 IRES2 g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t a c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a t g

2100 IRES2 a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c

BmgBI 2160 ATG 1 5 MVSKG IRES2 mCherry MATTMVSKG g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a c c a t g g t g a g c a a g g g c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t g g t a c c a c t c g t t c c c

2220 10 15 20 25 EEDNMAIIKEFMRFKVHMEG mCherry EEDNMAIIKEFMRFKVHMEG c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g g a g g g g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c c t c c c

2280 30 35 40 45 SVNGHEFEIEGEGEGRPYEG mCherry SVNGHEFEIEGEGEGRPYEG c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c

2340 50 55 60 65 TQTAKLKVTKGGPLPFAWDI mCherry G G T T C G A C T T C C A C T G G T T mCherry-R TQTAKLKVTKGGPLPFAWDI c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g g a c a t g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c c t g t a

2400 70 75 80 85 LSPQFMYGSKAYVKHPADIP mCherry LSPQFMYGSKAYVKHPADIP c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g c a c c c c g c c g a c a t c c c g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t c g t g g g g c g g c t g t a g g g

2460 90 95 100 105 DYLKLSFPEGFKWERVMNFE mCherry DYLKLSFPEGFKWERVMNFE c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t

SbfI 2520 110 115 120 125 DGGVVTVTQDSSLQDGEFIY mCherry DGGVVTVTQDSSLQDGEFIY g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g a c g g c g a g t t c a t c t a c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c t g c c g c t c a a g t a g a t

2580 130 135 140 145 KVKLRGTNFPSDGPVMQKKT mCherry C C C C G T A A T G C A G A A G A A G A mCherry-F KVKLRGTNFPSDGPVMQKKT c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c g t a a t g c a g a a g a a g a c g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g g c a t t a c g t c t t c t t c t g

2640 150 155 160 165 MGWEASSERMYPEDGALKGE mCherry MGWEASSERMYPEDGALKGE c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a c g g c g c c c t g a a g g g c g a g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t g c c g c g g g a c t t c c c g c t

BbvCI 2700 170 175 180 185 IKQRLKLKDGGHYDAEVKTT mCherry IKQRLKLKDGGHYDAEVKTT g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a c g c t g a g g t c a a g a c c a c c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t g c g a c t c c a g t t c t g g t g

PvuII 2760 190 195 200 205 YKAKKPVQLPGAYNVNIKLD mCherry YKAKKPVQLPGAYNVNIKLD c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a c g t c a a c a t c a a g t t g g a g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t g c a g t t g t a g t t c a a c c t

2820 210 215 220 225 ITSHNEDYTIVEQYERAEGR mCherry ITSHNEDYTIVEQYERAEGR c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a a c g c g c c g a g g g c c g g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t t g c g c g g c t c c c g g c

PaeR7IPspXIXhoIAgeI 2880 230 235 HSTGGMDELYK* mCherry HSTGGMDELYK* c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t a a a c c g g t g c t c g a g g t a c c g a t g a g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a t t t g g c c a c g a g c t c c a t g g c t a c t

2940 chimeric intron a t a g c t a a g g t c g a g g c c g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a t a t c g a t t c c a g c t c c g g c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t

3000 chimeric intron a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c

3060 chimeric intron WPRE t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c g a c a a t c a a c c t c t g g a t t a a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g c t g t t a g t t g g a g a c c t a a t

3120 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c

3180 WPRE (in frame with Factor Xa site) *HRQIMSNSGTHSENEG MPLYHAIASRMAFIFS a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g

3240 WPRE (in frame with Factor Xa site) GQIFGPQQRKILLQPGNDPL SLYKSWLLSLYEELWPVVRQ c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t

3300 WPRE (in frame with Factor Xa site) TAHHASHKSVCGGSTPANGG RGVVCTVFADATPTGWGIAT a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g

PfoI 3360 WPRE (in frame with Factor Xa site) GTLEKGPSESEGERNGRRFE TCQLLSGTFAFPLPIATAEL c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a

3420 WPRE (in frame with Factor Xa site) DGGAKGAAPCPSPQQASVIG IAACLARCWTGARLLGTDNS c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g

3480 WPRE (in frame with Factor Xa site) HHQRPLQRGKWPQEGTNGGP VVLSGKLTSFPWLLACVATW c g t g g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g c a c c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c

3540 WPRE (in frame with Factor Xa site) NQAPRGEAVDR 1 RGEI Factor Xa site ILRGTSFCYVPSALNPADLP g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g

3600 WPRE SRGLLPALRPLPRLRLRPQT t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g

3660 WPRE G G BGH-rev SRISLWAASPPGIRARYDQP g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g g a a t t c g a g c t c g g t a c g a t c a g c c c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c c t t a a g c t c g a g c c a t g c t a g t c g g

3720 bGH poly(A) signal A G C T G A C A C G G A A G A T BGH-rev RLCLLVASHLLFAPPPCLP* t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c

3780 bGH poly(A) signal a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a

3840 t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c

3900 T3 promoter G G A A A T C A C T C C C A A T T A A C G T3 g a t t g g g a a g a c a a t a g c c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c t a a c c c t t c t g t t a t c g g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c

Bsu36I 3960 M13 rev C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t a a t t c a c t c c t c a g g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a t t a a g t g a g g a g t c

4020 g t g c a g g c t g c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c g t c c g a c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g

BglII 4080 A G A A A A A G G G A G A C G G T T T T Bglob-pA-R c a c t g a g a t c t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t g t g a c t c t a g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a

4140 β- globin poly(A) signal c t g a c t t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g g a c t g a a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c

4200 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R t g t c t c t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t a c a g a g a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a

4260 t t g g t t t a g a g t t t g g c a a c a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a a a c c a a a t c t c a a a c c g t t g t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t

4320 t a a a g a g g t c a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a a t t t c t c c a g t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t

4380 g c c t t g a c t t g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a c g g a a c t g a a c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t

4440 t c c c t a a a a t t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c a g g g a t t t t a a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g

4500 c c a g t c a t a g c t g t c c c t c t t c t c t t a t g g a g a t c g g c c g g c c g c c g g c g t c g a c g c g c g g g t c a g t a t c g a c a g g g a g a a g a g a a t a c c t c t a g c c g g c c g g c g g c c g c a g c t g c g c g c

4560 ori c g g c c g c g g c g c g c c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a g c c g g c g c c g c g c g g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t

4620 ori T T T C T A T G G T C C G pBR322ori-F c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g

4680 ori C A A A G G G pBR322ori-F g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t

4740 ori c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t

4800 ori t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t

4860 ori g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t

4920 ori c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c

4980 ori t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c

5040 ori t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c

5100 ori c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c

5160 ori a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t

5220 c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a

5280 c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g

5340 285 280 275 270 *WHKILSAGIEAIQRNRE AmpR *WHKILSAGIEAIQRNRE t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g

5400 265 260 255 250 DMTAQSGTTYIVVIRSPKGD AmpR DMTAQSGTTYIVVIRSPKGD a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g

5460 245 240 235 230 PGLAAIIGRSGREGAGSKDA AmpR PGLAAIIGRSGREGAGSKDA t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g

5520 225 220 215 210 IFWGAPLASRLLPGAVKDAE AmpR IFWGAPLASRLLPGAVKDAE a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g

5580 205 200 195 190 MWDILQQRSALTLLEGTLLK AmpR MWDILQQRSALTLLEGTLLK c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a

FspI 5640 185 180 175 170 RLTTAMAVPMTTDREDNPIA AmpR RLTTAMAVPMTTDREDNPIA g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g

5700 165 160 155 150 ENLEPEWRDLRTVHDGMNHL AmpR ENLEPEWRDLRTVHDGMNHL t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t

PvuI 5760 145 140 135 130 FATLEKPGGITTLLLNAATN AmpR FATLEKPGGITTLLLNAATN a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a

5820 125 120 115 110 DSMTIAASCLERVTMGDTLH AmpR DSMTIAASCLERVTMGDTLH a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c

ScaI 5880 105 100 95 90 KETVPSYEVLDNQSYHIRRG AmpR KETVPSYEVLDNQSYHIRRG c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g

5940 85 80 75 70 LQEQGADIRSLVAGCLLVKF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R LQEQGADIRSLVAGCLLVKF g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t

6000 65 60 55 50 TSMMPFREEPRFSELIKGSN AmpR TSMMPFREEPRFSELIKGSN a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a

6060 45 40 35 30 LDLEIYGVRAGLQDEADKVK AmpR LDLEIYGVRAGLQDEADKVK g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a

6120 25 VLTEPH 20 15 10 signal sequence AFVPLCFAAFFPIL AmpR VLTEPHAFVPLCFAAFFPIL c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c

6180 5 1 signal sequence AVRFHQISM AmpR AmpR promoter AVRFHQISM g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t

6240 AmpR promoter t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a

6300 AmpR promoter a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a

6360 A A T A G G C G T A T C A C G A G G C pBRforEco c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t a c g c g c g c g c c g g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a t g c g c g c g c g g c

6392 g c g g c g c g c c g t c g a c g g c c g g c c g c g g c c g c c g c c g c g c g g c a g c t g c c g g c c g g c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.