Addgene pRDAX-334 N-Tag miRFP670[2] L20 {Esp3I(2x)} EGFP - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pRDAX-334 N-Tag miRFP670[2] L20 {Esp3I(2x)} EGFP


Map View

pBRforEco (6480 .. 6498) Amp-R (6070 .. 6089) ScaI (6000) PvuI (5890) FspI (5742) AhdI (5520) AlwNI (5043) HaeII (4875) pBR322ori-F (4768 .. 4787) rbglobpA-R (4308 .. 4327) Bglob-pA-R (4189 .. 4208) BglII (4186) M13/pUC Reverse (4084 .. 4106) M13 Reverse (4071 .. 4087) M13 rev T3 (4032 .. 4052) T3 promoter BGH-rev (3819 .. 3836) WPRE-R (3257 .. 3277) StuI * (13) SpeI (167) CMV enhancer SnaBI (508) CMV-F (687 .. 707) LNCX (733 .. 757) HindIII (763) AflII (835) Kozak sequence KflI - PpuMI (1037) PflFI - Tth111I (1124) BlpI (1358) BamHI (1625) BspEI * (1628) AvrII (1862) IRES reverse (1878 .. 1895) PaqCI (2050) DraIII (2074) IRES-F (2105 .. 2124) BmgBI (2254) EGFP-N (2343 .. 2364) EXFP-R (2604 .. 2623) EGFP-C (2951 .. 2972) AgeI (3018) PaeR7I - PspXI - XhoI (3025) pRDAX-334 N-Tag miRFP670[2] L20 {Esp3I(2x)} EGFP 6552 bp

Sequence View

StuI* 60 g c c t g c c t c c a g g c c t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a c g g a c g g a g g t c c g g a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t

120 a a t c a a t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t t a g t t a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t

SpeI 180 CMV enhancer t a t t g g c t c a t g t c c a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a a t a a c c g a g t a c a g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t

240 CMV enhancer g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a

300 CMV enhancer t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a

360 CMV enhancer g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t

420 CMV enhancer t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g

480 CMV enhancer t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c

SnaBI 540 CMV enhancer CMV promoter g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c

600 CMV promoter g t t t t g g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c a a a a c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a

660 CMV promoter c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t

720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a t g t c g t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t t a c a g c a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a

HindIII 780 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a

AflII 840 t t a t c a c a g t t a a a t t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g a a t a g t g t c a a t t t a a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c

900 chimeric intron c t g c a g a a g t t g g t c g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a g a c g t c t t c a a c c a g c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t

960 chimeric intron a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g

1020 chimeric intron Kozak sequence a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g c t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c g

PpuMIKflI 1080 Kozak sequence MGRAGPSIDLSGTLAPALE c a c c a t g g g c a g a g c g g g a c c c a g t a t c g a c c t g t c t g g g a c a c t c g c a c c a g c a c t g g a g t g g t a c c c g t c t c g c c c t g g g t c a t a g c t g g a c a g a c c c t g t g a g c g t g g t c g t g a c c t

Tth111IPflFI 1140 RIRTAGSLRALCDDTVLLFQ g c g c a t a a g a a c t g c t g g a t c a c t g a g g g c c c t c t g t g a c g a c a c a g t c t t g c t c t t t c a c g c g t a t t c t t g a c g a c c t a g t g a c t c c c g g g a g a c a c t g c t g t g t c a g a a c g a g a a a g t

1200 QCTGYDRVMVYRFDEQGHGL g c a a t g t a c g g g c t a t g a t c g a g t g a t g g t g t a c c g a t t c g a c g a a c a g g g t c a t g g t c t c g t t a c a t g c c c g a t a c t a g c t c a c t a c c a c a t g g c t a a g c t g c t t g t c c c a g t a c c a g a

1260 VFSECHVPGLESYFGNRYPS g g t c t t t t c c g a g t g t c a c g t g c c t g g c c t g g a g a g c t a c t t t g g c a a c c g c t a t c c c t c c c a g a a a a g g c t c a c a g t g c a c g g a c c g g a c c t c t c g a t g a a a c c g t t g g c g a t a g g g a g

1320 STVPQMARQLYVRQRVRVLV t t c t a c c g t a c c c c a g a t g g c c a g g c a a c t t t a c g t c c g g c a a c g g g t c a g a g t g c t g g t a a g a t g g c a t g g g g t c t a c c g g t c c g t t g a a a t g c a g g c c g t t g c c c a g t c t c a c g a c c a

BlpI 1380 DVTYQPVPLEPRLSPLTGRD g g a t g t g a c c t a c c a g c c t g t g c c a c t c g a a c c t c g g c t g a g c c c a c t c a c t g g a c g g g a c c t a c a c t g g a t g g t c g g a c a c g g t g a g c t t g g a g c c g a c t c g g g t g a g t g a c c t g c c c t

1440 LDMSGCFLRSMSPCHLQFLK t c t g g a c a t g a g c g g c t g c t t c c t g a g g t c c a t g t c a c c t t g c c a t c t g c a g t t c t t g a a a g a c c t g t a c t c g c c g a c g a a g g a c t c c a g g t a c a g t g g a a c g g t a g a c g t c a a g a a c t t

1500 DMGVRATLAVSLVVGGKLWG g g a c a t g g g c g t t c g a g c c a c a c t c g c a g t c t c a c t g g t t g t a g g c g g g a a a c t g t g g g g c c t g t a c c c g c a a g c t c g g t g t g a g c g t c a g a g t g a c c a a c a t c c g c c c t t t g a c a c c c c

1560 LVVCHHYLPRFIRFELRAIC t c t t g t g g t g t g t c a c c a c t a c c t t c c c a g g t t c a t c c g g t t c g a g t t g a g a g c c a t c t g a g a a c a c c a c a c a g t g g t g a t g g a a g g g t c c a a g t a g g c c a a g c t c a a c t c t c g g t a g a c

1620 KRLAERIATRITALESGSPG c a a a c g t t t g g c c g a g a g a a t a g c c a c c c g c a t t a c t g c t c t g g a a t c c g g g a g t c c t g g g t t t g c a a a c c g g c t c t c t t a t c g g t g g g c g t a a t g a c g a g a c c t t a g g c c c t c a g g a c c

BamHIBspEI* 1680 GGSGGSGSSASGGSTSRDAE c g g t g g a t c c g g a g g c t c t g g g a g c t c t g c c a g c g g t g g c a g c a c c t c c c g a g a c g c c g a g c c a c c t a g g c c t c c g a g a c c c t c g a g a c g g t c g c c a c c g t c g t g g a g g g c t c t g c g g c t

1740 IRES2 ARRLLRIQIRPSPSPPPNVT g g c a c g c c g t c t c c t a a g a a t t c a g a t c c g c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c c c g t g c g g c a g a g g a t t c t t a a g t c t a g g c g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g

1800 IRES2 GRSRLE* t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a

1860 IRES2 a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a

AvrII 1920 IRES2 G A G A G C G G T T T C C T T A C G IRES reverse t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t

1980 IRES2 a g c a g t t c c t c t g g t a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a t c g t c a a g g a g a c c a t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t

2040 IRES2 g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g

DraIIIPaqCI 2100 IRES2 a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a

2160 IRES2 T G G C T C T C C T C A A G C G T A T T IRES-F c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t

2220 IRES2 t t g t a t g g g a t c t g a t c t g g g g c c t c g g t a c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a t g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a

BmgBI 2280 IRES2 a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c

2340 ATG 1 M 1a V 5 10 SKGEELFTGVVP IRES2 EGFP 1 M 1a V 5 10 SKGEELFTGVVP EGFP MATTMVSKGEELFTGVVP a t a a t a t g g c c a c a a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t a t t a t a c c g g t g t t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t

2400 15 20 25 30 ILVELDGDVNGHKFSVSGEG EGFP 15 20 25 30 ILVELDGDVNGHKFSVSGEG EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N ILVELDGDVNGHKFSVSGEG t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c

2460 35 40 45 50 EGDATYGKLTLKFICTTGKL EGFP 35 40 45 50 EGDATYGKLTLKFICTTGKL EGFP EGDATYGKLTLKFICTTGKL a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g

2520 55 60 65 70 PVPWPTLVTTLTYGVQCFSR EGFP 55 60 65 70 PVPWPTLVTTLTYGVQCFSR EGFP PVPWPTLVTTLTYGVQCFSR c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a

2580 75 80 85 90 YPDHMKQHDFFKSAMPEGYV EGFP 75 80 85 90 YPDHMKQHDFFKSAMPEGYV EGFP YPDHMKQHDFFKSAMPEGYV a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g

2640 95 100 105 110 QERTIFFKDDGNYKTRAEVK EGFP 95 100 105 110 QERTIFFKDDGNYKTRAEVK EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R QERTIFFKDDGNYKTRAEVK a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a

2700 115 120 125 130 FEGDTLVNRIELKGIDFKED EGFP 115 120 125 130 FEGDTLVNRIELKGIDFKED EGFP FEGDTLVNRIELKGIDFKED t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c

2760 135 140 145 150 GNILGHKLEYNYNSHNVYIM EGFP 135 140 145 150 GNILGHKLEYNYNSHNVYIM EGFP GNILGHKLEYNYNSHNVYIM g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c

2820 155 160 165 170 ADKQKNGIKVNFKIRHNIED EGFP 155 160 165 170 ADKQKNGIKVNFKIRHNIED EGFP ADKQKNGIKVNFKIRHNIED c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c

2880 175 180 185 190 GSVQLADHYQQNTPIGDGPV EGFP 175 180 185 190 GSVQLADHYQQNTPIGDGPV EGFP GSVQLADHYQQNTPIGDGPV g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g

2940 195 200 205 210 LLPDNHYLSTQSALSKDPNE EGFP 195 200 205 210 LLPDNHYLSTQSALSKDPNE EGFP LLPDNHYLSTQSALSKDPNE t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t

3000 215 220 225 230 KRDHMVLLEFVTAAGITLGM EGFP 215 220 225 230 KRDHMVLLEFVTAAGITLGM EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C KRDHMVLLEFVTAAGITLGM a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c

PaeR7IPspXIXhoIAgeI 3060 235 DELYK* EGFP 235 DELYK * EGFP DELYK* a c g a g c t g t a c a a g t a a a c c g g t g c t c g a g g t a c c g a t g a a t a g c t a a g g t c g a g g c c g c t g c t c g a c a t g t t c a t t t g g c c a c g a g c t c c a t g g c t a c t t a t c g a t t c c a g c t c c g g c g

3120 chimeric intron a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a a c t g g g c t t g t c g a t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t t g a c c c g a a c a g c t

3180 chimeric intron g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g a c t g t a g g t g a a a c

3240 chimeric intron WPRE c c t t t c t c t c c a c a g g t g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a g g a a a g a g a g g t g t c c a c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t

3300 WPRE *HR A C A A C G A G G A A A A T G C G A T A C WPRE-R MP c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a

3360 WPRE (in frame with Factor Xa site) QIMSNSGTHSENEGGQIFGP LYHAIASRMAFIFSSLYKSW t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a

3420 WPRE (in frame with Factor Xa site) QQRKILLQPGNDPLTAHHAS LLSLYEELWPVVRQRGVVCT t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c

3480 WPRE (in frame with Factor Xa site) HKSVCGGSTPANGGGTLEKG VFADATPTGWGIATTCQLLS t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c

3540 WPRE (in frame with Factor Xa site) PSESEGERNGRRFEDGGAKG GTFAFPLPIATAELIAACLA g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g

3600 WPRE (in frame with Factor Xa site) AAPCPSPQQASVIGHHQRPL RCWTGARLLGTDNSVVLSGK g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g

3660 WPRE (in frame with Factor Xa site) QRGKWPQEGTNGGPNQAPRG LTSFPWLLACVATWILRGTS t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a

3720 WPRE EAVDR 1 RGEI Factor Xa site FCYVPSALNPADLPSRGLLP t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c

3780 WPRE ALRPLPRLRLRPQTSRISLW c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c

3840 WPRE bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev AASPPGIRARYDQPRLCLLV c c g c c t c c c c g c c t g g a a t t c g a g c t c g g t a c g a t c a g c c t c g a c t g t g c c t t c t a g t t g g g c g g a g g g g c g g a c c t t a a g c t c g a g c c a t g c t a g t c g g a g c t g a c a c g g a a g a t c a a c

3900 bGH poly(A) signal ASHLLFAPPPCLP* c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g

3960 bGH poly(A) signal c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g

4020 t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c c c a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g g g

4080 T3 promoter M13 rev G G A A A T C A C T C C C A A T T A A C G T3 C A G T A T C G A C M13 Reverse a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c

4140 M13 rev A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t t t c c t g t g t g a a a t t g t t a t c c g c t a a t t c a c t c c t c a g g t g c a g g c t g c c t a t c a g a a a a a g g a c a c a c t t t a a c a a t a g g c g a t t a a g t g a g g a g t c c a c g t c c g a c g g a t a g t c t t

BglII 4200 A G A A A A A G G G A G Bglob-pA-R g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a g a t c t t t t t c c c t c c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t c t a g a a a a a g g g a g

4260 β- globin poly(A) signal A C G G T T T T Bglob-pA-R t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t t c t g g c t a a t a a a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a a g a c c g a t t a t t t c

4320 β- globin poly(A) signal G T G A G C C T T C C T G rbglobpA-R g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g

4380 T A T A C C C rbglobpA-R a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t t a g a g t t t g g c a a c t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a a t c t c a a a c c g t t g

4440 a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a g g t c a t c a g t a t a t t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t c c a g t a g t c a t a t a

4500 g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g a c t t g a g g t t a g a t c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c t g a a c t c c a a t c t a

4560 t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a a a a t t t t c c t t a c a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t t t t a a a a g g a a t g t

4620 t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c a t a g c t g t c c c t c t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g t a t c g a c a g g g a g a

4680 t c t c t t a t g g a g a t c g g c c g g c c g c c g g c g t c g a c g c g c g c g g c c g c g g c g c g c c g t t g c a g a g a a t a c c t c t a g c c g g c c g g c g g c c g c a g c t g c g c g c g c c g g c g c c g c g c g g c a a c g

4740 ori t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g

4800 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g

4860 ori t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a

HaeII 4920 ori c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c

4980 ori t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a

5040 ori c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c

AlwNI 5100 ori c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a

5160 ori g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g

5220 ori c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t

5280 ori g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c

5340 a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t

5400 t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t

5460 285 *WHKI AmpR *WHKI g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t

AhdI 5520 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g

5580 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t

5640 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t

5700 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a

FspI 5760 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c

5820 180 175 170 165 VPMTTDREDNPIAENLEPEW AmpR VPMTTDREDNPIAENLEPEW c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g

5880 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c

PvuI 5940 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c

ScaI 6000 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a

6060 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a

6120 80 75 70 65 IRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRSLVAGCLLVKFTSMMPFR c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g

6180 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g

6240 40 35 30 25 VRAGLQDEADKVKVLTEPH A AmpR VRAGLQDEADKVKVLTEPHA c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c

6300 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t

6360 1 signal sequ... SM AmpR AmpR promoter SM t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t

6420 AmpR promoter g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g

6480 A pBRforEco c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t

6540 A T A G G C G T A T C A C G A G G C pBRforEco a t a g g c g t a t c a c g a g g c c c t t t c g t c t a c g c g c g c g c c g g c g g c g c g c c g t c g a c g g c c t a t c c g c a t a g t g c t c c g g g a a a g c a g a t g c g c g c g c g g c c g c c g c g c g g c a g c t g c c g g

6552 g g c c g c g g c c g c c c g g c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.