Addgene pJUMP19-L1U1H09_T Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pJUMP19-L1U1H09_T Sequencing Result

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PacI (5) AscI (3858) pGEX 3' (3497 .. 3519) PfoI (3496) PflFI - Tth111I (3397) pRS-marker (3337 .. 3356) BstAPI (3324) BspQI - SapI (3258) L4440 (3239 .. 3256) PciI (3141) pBR322ori-F (2986 .. 3005) BssSI - BssSαI (2968) FseI (2469) BsaHI (2344) PshAI (2212) ApaI - BanII (2047) AvrII (30) EcoRI (101) XbaI (116) NruI (203) SpeI (210) BioBrick suffix PstI (228) RBS GFP-R (321 .. 349) NcoI (456) MscI (461) BsrGI (566) PmlI (615) HpaI (779) MfeI (850) BstBI (912) GFP-F (944 .. 964) EcoO109I - KflI - PpuMI (1144) SwaI (1164) BspHI (1250) SspI (1285) BsmI (1307) FspI (1370) TsoI (1603) BstEII (1617) SacII (1697) BfuAI - BspMI (1712) RsrII (1726) BsrDI (1856) PspOMI (2043) pJUMP19-L1U1H09_T 3976 bp

Sequence View

AvrIIPacI 5ʹ 3ʹ 60  BBa_J23100 t t a a t t a a g g a g t t g a c g g c t a g c t c a g t c c t a g g t a c a g t g c t a g c g t a c t t a a c t t c t a a t t a a t t c c t c a a c t g c c g a t c g a g t c a g g a t c c a t g t c a c g a t c g c a t g a a t t g a a g a

XbaIEcoRI 120 BioBrick prefix g a c t g a g t t g c a c g c t t a a c t t c t g a c t g a g t t g c a c g c t g a a t t c g c g g c c g c t t c t a g c t g a c t c a a c g t g c g a a t t g a a g a c t g a c t c a a c g t g c g a c t t a a g c g c c g g c g a a g a t c

180 BioBrick prefix a g g g t c t g c g a t g t t t g g t c t c t g c t t t c g a c g a c g a t g t t c g c a t c g t c g t t t t t t t t t t c c c a g a c g c t a c a a a c c a g a g a c g a a a g c t g c t g c t a c a a g c g t a g c a g c a a a a a a a a a

PstISpeINruI 240 BioBrick suffix t g t t c g c t t g a g a c c t t t c a t c g c g a c c t a c t a g t a g c g g c c g c t g c a g g g a g t a a c t t c a c a a g c g a a c t c t g g a a a g t a g c g c t g g a t g a t c a t c g c c g g c g a c g t c c c t c a t t g a a g

300 K RBS GFP K GFP (S65T) MVARK t g a c t g a g t t g c a t a c t t g t t t a a c t t t a a g a a g g a g a t a t a c a a t g g t a g c c c g t a a a g a c t g a c t c a a c g t a t g a a c a a a t t g a a a t t c t t c c t c t a t a t g t t a c c a t c g g g c a t t t c

360 5 10 15 20 GEELFTGVVPILVELDGDVN GFP 5 10 15 20 GEELFTGVVPILVELDGDVN GFP (S65T) C A A C A G G G T T A A G A A C A A C T T A A T C T A C C GFP-R GEELFTGVVPILVELDGDVN g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t g t t g a a t t a g a t g g t g a t g t t a a t g c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a c a a c t t a a t c t a c c a c t a c a a t t a c

420 25 30 35 40 GHKFSVSGEGEGDATYGKLT GFP 25 30 35 40 GHKFSVSGEGEGDATYGKLT GFP (S65T) GHKFSVSGEGEGDATYGKLT g g c a c a a a t t t t c t g t c a g t g g a g a g g g t g a a g g t g a t g c a a c a t a c g g a a a a c t t a c c c c c g t g t t t a a a a g a c a g t c a c c t c t c c c a c t t c c a c t a c g t t g t a t g c c t t t t g a a t g g g

MscINcoI 480 45 50 55 60 LKFICTTGKLPVPWPTLVTT GFP 45 50 55 60 LKFICTTGKLPVPWPTLVTT GFP (S65T) LKFICTTGKLPVPWPTLVTT t t a a a t t t a t t t g c a c t a c t g g a a a a c t a c c t g t t c c a t g g c c a a c a c t t g t c a c t a c t t a a t t t a a a t a a a c g t g a t g a c c t t t t g a t g g a c a a g g t a c c g g t t g t g a a c a g t g a t g a a

540 65 70 75 80 FGYGVQCFARYPDHMKQHDF GFP 65 70 75 80 FGYGVQCFARYPDHMKQHDF GFP (S65T) FGYGVQCFARYPDHMKQHDF t c g g t t a t g g t g t t c a a t g c t t t g c g a g a t a c c c a g a t c a t a t g a a a c a g c a t g a c t t t t a g c c a a t a c c a c a a g t t a c g a a a c g c t c t a t g g g t c t a g t a t a c t t t g t c g t a c t g a a a a

BsrGI 600 85 90 95 100 FKSAMPEGYVQERTIFFKDD GFP 85 90 95 100 FKSAMPEGYVQERTIFFKDD GFP (S65T) FKSAMPEGYVQERTIFFKDD t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a a g a a c t a t a t t t t t c a a a g a t g a c g a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t t c t t g a t a t a a a a a g t t t c t a c t g c

PmlI 660 105 110 115 120 GNYKTRAEVKFEGDTLVNRI GFP 105 110 115 120 GNYKTRAEVKFEGDTLVNRI GFP (S65T) GNYKTRAEVKFEGDTLVNRI g g a a c t a c a a g a c a c g t g c t g a a g t c a a g t t t g a a g g t g a t a c c c t t g t t a a t a g a a t c g c c t t g a t g t t c t g t g c a c g a c t t c a g t t c a a a c t t c c a c t a t g g g a a c a a t t a t c t t a g c

720 125 130 135 140 ELKGIDFKEDGNILGHKLEY GFP 125 130 135 140 ELKGIDFKEDGNILGHKLEY GFP (S65T) ELKGIDFKEDGNILGHKLEY a g t t a a a a g g t a t t g a t t t t a a a g a a g a t g g a a a c a t t c t t g g a c a c a a a t t g g a a t a c a t c a a t t t t c c a t a a c t a a a a t t t c t t c t a c c t t t g t a a g a a c c t g t g t t t a a c c t t a t g t

HpaI 780 145 150 155 160 NYNSHNVYIMADKQKNGIKV GFP 145 150 155 160 NYNSHNVYIMADKQKNGIKV GFP (S65T) NYNSHNVYIMADKQKNGIKV a c t a t a a c t c a c a c a a t g t a t a c a t c a t g g c a g a c a a a c a a a a g a a t g g a a t c a a a g t t a t g a t a t t g a g t g t g t t a c a t a t g t a g t a c c g t c t g t t t g t t t t c t t a c c t t a g t t t c a a t

840 165 170 175 180 NFKIRHNIEDGSVQLADHYQ GFP 165 170 175 180 NFKIRHNIEDGSVQLADHYQ GFP (S65T) NFKIRHNIEDGSVQLADHYQ a c t t c a a a a t t a g a c a c a a c a t t g a a g a t g g a a g c g t t c a a c t a g c a g a c c a t t a t c a a c t g a a g t t t t a a t c t g t g t t g t a a c t t c t a c c t t c g c a a g t t g a t c g t c t g g t a a t a g t t g

MfeI 900 185 190 195 200 QNTPIGDGPVLLPDNHYLST GFP 185 190 195 200 QNTPIGDGPVLLPDNHYLST GFP (S65T) QNTPIGDGPVLLPDNHYLST a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c c a g a c a a c c a t t a c c t g t c c a c a c t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t g g t c t g t t g g t a a t g g a c a g g t g t g

BstBI 960 205 210 215 220 QSALSKDPNEKRDHMVLLEF GFP 205 210 215 220 QSALSKDPNEKRDHMVLLEF GFP (S65T) G G T C C T T C T T G A G T T T G GFP-F QSALSKDPNEKRDHMVLLEF a a t c t g c c c t t t c g a a a g a t c c c a a c g a a a a g a g a g a t c a c a t g g t c c t t c t t g a g t t t g t t a g a c g g g a a a g c t t t c t a g g g t t g c t t t t c t c t c t a g t g t a c c a g g a a g a a c t c a a a c

1020 225 230 235 VTAAGITHGMDELYK GS* GFP 225 230 235 VTAAGITHGMDELYK GS* GFP (S65T) T A A C GFP-F VTAAGITHGMDELYKGS* t a a c a g c t g c t g g g a t t a c a c a t g g c a t g g a t g a a c t a t a c a a a g g t t c g t a a c t t c t g a a t t g t c g a c g a c c c t a a t g t g t a c c g t a c c t a c t t g a t a t g t t t c c a a g c a t t g a a g a c t

1080 lambda t0 terminator c t g a g t t g c a c g c t c t a g t c t t g g a c t c c t g t t g a t a g a t c c a g t a a t g a c c t c a g a a c t g a c t c a a c g t g c g a g a t c a g a a c c t g a g g a c a a c t a t c t a g g t c a t t a c t g g a g t c t t g a

1140 lambda t0 terminator c c a t c t g g a t t t g t t c a g a a c g c t c g g t t g c c g c c g g g c g t t t t t t a t t g g t g a g a a t c c g g t a g a c c t a a a c a a g t c t t g c g a g c c a a c g g c g g c c c g c a a a a a a t a a c c a c t c t t a g g

SwaIEcoO109IPpuMIKflI 1200 a g g g g t c c c c a a t a a t t a c g a t t t a a a t t a g t a g c c c g c c t a a t g a g c g g g c t t t t t t t t t c c c c a g g g g t t a t t a a t g c t a a a t t t a a t c a t c g g g c g g a t t a c t c g c c c g a a a a a a a a

BspHI 1260 AmpR promoter *IHIREHSL a a t t c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t t a a g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t

BsmISspI 1320 1 5 signal sequence MSIQHF AmpR promoter AmpR MSIQHF LGSLHKLLISFPLTHANLMK t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g c a t t c a g c a t t t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c g t a a g t c g t a a a a

FspI 1380 10 15 20 signal sequence RVALIPFFAAFCLPVFA 25 HPE AmpR RVALIPFFAAFCLPVFAHPE THRQNRKKRRKAQRHKRMRF c g t g t g g c g c t g a t t c c g t t t t t t g c g g c g t t t t g c c t g c c g g t g t t t g c g c a t c c g g a a g c a c a c c g c g a c t a a g g c a a a a a a c g c c g c a a a a c g g a c g g c c a c a a a c g c g t a g g c c t t

1440 30 35 40 45 TLVKVKDAEDQLGARVGYIE AmpR TLVKVKDAEDQLGARVGYIE GQHFHFIRFILQTRAHAINF a c c c t g g t g a a a g t g a a a g a t g c g g a a g a t c a a c t g g g t g c g c g c g t g g g c t a t a t t g a a t g g g a c c a c t t t c a c t t t c t a c g c c t t c t a g t t g a c c c a c g c g c g c a c c c g a t a t a a c t t

1500 50 55 60 65 LDLNSGKILESFRPEERFPM AmpR LDLNSGKILESFRPEERFPM QIQVAAFNQFRKTRFFTKRH c t g g a t c t g a a c a g c g g c a a a a t t c t g g a a t c t t t t c g t c c g g a a g a a c g t t t t c c g a t g g a c c t a g a c t t g t c g c c g t t t t a a g a c c t t a g a a a a g c a g g c c t t c t t g c a a a a g g c t a c

1560 70 75 80 85 MSTFKVLLCGAVLSRVDAGQ AmpR MSTFKVLLCGAVLSRVDAGQ HAGKFHQQATRNQATHIRAL a t g a g c a c c t t t a a a g t g c t g c t g t g c g g t g c g g t t c t g a g c c g t g t g g a t g c g g g c c a g t a c t c g t g g a a a t t t c a c g a c g a c a c g c c a c g c c a a g a c t c g g c a c a c c t a c g c c c g g t c

BstEIITsoI 1620 90 95 100 105 EQLGRRIHYSQNDLVEYSPV AmpR EQLGRRIHYSQNDLVEYSPV FLQATTNMIALVIQHFIARH g a a c a a c t g g g c c g t c g t a t t c a t t a t a g c c a g a a c g a t c t g g t g g a a t a t a g c c c g g t g c t t g t t g a c c c g g c a g c a t a a g t a a t a t c g g t c t t g c t a g a c c a c c t t a t a t c g g g c c a c

1680 110 115 120 125 TEKHLTDGMTVRELCSAAIT AmpR TEKHLTDGMTVRELCSAAIT GFFMQGIAHGHTFQAARRNG a c c g a a a a a c a t c t g a c c g a t g g c a t g a c c g t g c g t g a a c t g t g c a g c g c g g c g a t t a c c t g g c t t t t t g t a g a c t g g c t a c c g t a c t g g c a c g c a c t t g a c a c g t c g c g c c g c t a a t g g

RsrIIBspMIBfuAISacII 1740 130 135 140 145 MSDNTAANLLLTTIGGPKEL AmpR MSDNTAANLLLTTIGGPKEL HAIVGRRVQQQRGNATRFFQ a t g a g c g a t a a c a c c g c g g c g a a c c t g c t g c t g a c g a c c a t t g g c g g t c c g a a a g a a c t g t a c t c g c t a t t g t g g c g c c g c t t g g a c g a c g a c t g c t g g t a a c c g c c a g g c t t t c t t g a c

1800 150 155 160 165 TAFLHNMGDHVTRLDRWEPE AmpR TAFLHNMGDHVTRLDRWEPE GRKQMVHAIMHGTQITPFRF a c c g c g t t t c t g c a t a a c a t g g g c g a t c a t g t g a c c c g t c t g g a t c g t t g g g a a c c g g a a t g g c g c a a a g a c g t a t t g t a c c c g c t a g t a c a c t g g g c a g a c c t a g c a a c c c t t g g c c t t

BsrDI 1860 170 175 180 185 LNEAIPNDERDTTMPAAMAT AmpR LNEAIPNDERDTTMPAAMAT QVFRNRVIFTIGGHRCCHRG c t g a a c g a a g c g a t t c c g a a c g a t g a a c g t g a t a c c a c c a t g c c g g c a g c a a t g g c g a c c g a c t t g c t t c g c t a a g g c t t g c t a c t t g c a c t a t g g t g g t a c g g c c g t c g t t a c c g c t g g

1920 190 195 200 205 TLRKLLTGELLTLASRQQLI AmpR TLRKLLTGELLTLASRQQLI GQTFQQRTLQQGQCAALLQN a c c c t g c g t a a a c t g c t g a c g g g t g a g c t g c t g a c c c t g g c a a g c c g c c a g c a a c t g a t t t g g g a c g c a t t t g a c g a c t g c c c a c t c g a c g a c t g g g a c c g t t c g g c g g t c g t t g a c t a a

1980 210 215 220 225 DWMEADKVAGPLLRSALPAG AmpR DWMEADKVAGPLLRSALPAG IPHFRIFHRTRQQTARQRSA g a t t g g a t g g a a g c g g a t a a a g t g g c g g g t c c g c t g c t g c g t a g c g c g c t g c c g g c t g g c c t a a c c t a c c t t c g c c t a t t t c a c c g c c c a g g c g a c g a c g c a t c g c g c g a c g g c c g a c c g

2040 230 235 240 245 WFIADKSGAGERGSRGIIAA AmpR WFIADKSGAGERGSRGIIAA PKNRIFATRAFTAATANNRR t g g t t t a t t g c g g a t a a a a g c g g t g c g g g c g a a c g t g g c a g c c g t g g c a t t a t t g c g g c g a c c a a a t a a c g c c t a t t t t c g c c a c g c c c g c t t g c a c c g t c g g c a c c g t a a t a a c g c c g c

PspOMIBanIIApaI 2100 250 255 260 265 LGPDGKPSRIVVIYTTGSQA AmpR LGPDGKPSRIVVIYTTGSQA QARITFRATNHHNIGGAALR c t g g g c c c g g a t g g t a a a c c g a g c c g t a t t g t g g t g a t t t a t a c c a c c g g c a g c c a g g c g g a c c c g g g c c t a c c a t t t g g c t c g g c a t a a c a c c a c t a a a t a t g g t g g c c g t c g g t c c g c

2160 270 275 280 285 TMDERNRQIAEIGASLIKHW AmpR TMDERNRQIAEIGASLIKHW RHIFTVTLNRFNARAQNFMP a c g a t g g a t g a a c g t a a c c g t c a g a t t g c g g a a a t t g g c g c g a g c c t g a t t a a a c a t t g g t g c t a c c t a c t t g c a t t g g c a g t c t a a c g c c t t t a a c c g c g c t c g g a c t a a t t t g t a a c c

PshAI 2220 * AmpR fd terminator * LGICNFAGKPAKKKPRGKDK t a a a c c g a t a c a a t t a a a g g c t c c t t t t g g a g c c t t t t t t t t t g g a c g a c c c t t g t c c t t a t t t g g c t a t g t t a a t t t c c g a g g a a a a c c t c g g a a a a a a a a a c c t g c t g g g a a c a g g a a

2280 EAAYGQKPTM t t c c g c t g c a t a a c c c t g c t t c g g g g t c a t t a t a g c g a t t t t t t c g g t a t a t c c a t c c t t a a g g c g a c g t a t t g g g a c g a a g c c c c a g t a a t a t c g c t a a a a a a g c c a t a t a g g t a g g a a

2340 t t t c g c a c g a t a t a c a g g a t t t t g c c a a a g g g t t c g t g t a g a c t t t c c t t g g t g t a t c c a a a a g c g t g c t a t a t g t c c t a a a a c g g t t t c c c a a g c a c a t c t g a a a g g a a c c a c a t a g g t

BsaHI 2400 oriT a c g g c g t c a g c c g g g c a g g a t a g g t g a a g t a g g c c c a c c c g c g a g c g g g t g t t c c t t c t t t g c c g c a g t c g g c c c g t c c t a t c c a c t t c a t c c g g g t g g g c g c t c g c c c a c a a g g a a g a a

2460 oriT c a c t g t c c c t t a t t c g c a c c t g g c g g t g c t c a a c g g g a a t c c t g c t c t g c g a g g c t g g c c g t g a c a g g g a a t a a g c g t g g a c c g c c a c g a g t t g c c c t t a g g a c g a g a c g c t c c g a c c g g

FseI 2520 oriT ori g t a g g c c g g c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t c a t c c g g c c g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a

2580 ori a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t

2640 ori a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g

2700 ori t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g

2760 ori a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a

2820 ori t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c

2880 ori g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t

2940 ori g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a

BssSαIBssSI 3000 ori G G G A A A C G C C T G G T A pBR322ori-F a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t

3060 ori T C T T T pBR322ori-F t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g

3120 ori g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g

PciI 3180 c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t

3240 A G L4440 c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c

BspQISapI 3300 bom C G A G T C A G T G A G C G A G L4440 c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a

BstAPI 3360 bom A T G T T A G A C G A G A C T A C G G C pRS-marker g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t

Tth111IPflFI 3420 bom g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c g a c a c c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g c t g t g

3480 c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t

PfoI 3540 60 *LNEGDDGF rop C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c a a g c t g t g a c a g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a g t t c g a c a c t g t c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t

3600 55 50 45 40 RALCSRYLEDAHDHLSECID rop c g c g c g a g g c a g c t g c g g t a a a g c t c a t c a g c g t g g t c g t g c a g c g a t t c a c a g a t g t c t g c g c g c t c c g t c g a c g c c a t t t c g a g t a g t c g c a c c a g c a c g t c g c t a a g t g t c t a c a g a

3660 35 30 25 20 AQEDADLENLKELLTLTQSR rop g c c t g t t c a t c c g c g t c c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c t t c t g c g g a c a a g t a g g c g c a g g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g a a g a c

3720 15 10 5 1 IFRAMNLATKEQKTM rop a t a a a g c g g g c c a t g t t a a g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g t g t a a t a t t t c g c c c g g t a c a a t t c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c a c a t t

3780 g g g g g a t t t c t g t t c a t g g g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a c g a t a c c c c c t a a a g a c a a g t a c c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t g c t a t

3840 c g g g t t a c t g a t g a t g a a c a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c t g g c g g c c c a a t g a c t a c t a c t t g t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g a c c g c

AscI 3900 rrnB T1 terminator g t a t g g a t g c g g c g g g g g c g c g c c c a g c t g t c t a g g g c g g c g g a t t t g t c c t a c t c a g g a c a t a c c t a c g c c g c c c c c g c g c g g g t c g a c a g a t c c c g c c g c c t a a a c a g g a t g a g t c c t

3960 rrnB T1 terminator g a g c g t t c a c c g a c a a a c a a c a g a t a a a a c g a a a g g c c c a g t c t t t c g a c t g a g c c t t t c c t c g c a a g t g g c t g t t t g t t g t c t a t t t t g c t t t c c g g g t c a g a a a g c t g a c t c g g a a a g

3ʹ 5ʹ 3976 rrnB T1 terminator g t t t t a t t t g a t g c c t c a a a a t a a a c t a c g g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.