Addgene pBacMam2-DiEx-LIC-N entry vector Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pBacMam2-DiEx-LIC-N entry vector Sequencing Result


Map View

MluI (23) SphI (0) TK-pA-R (15,513 .. 15,532) Pc promoter L4440 (13,740 .. 13,757) pBR322ori-F (13,487 .. 13,506) FspI (12,531) PvuI (12,385) Amp-R (12,185 .. 12,204) AmpR promoter F1ori-F (11,678 .. 11,699) F1ori-R (11,469 .. 11,488) Tn7L EBV-rev (10,961 .. 10,980) SV40pA-R (10,907 .. 10,926) HpaI (10,869) PluTI (10,672) SfoI (10,670) NarI (10,669) KasI (10,668) WPRE-R (10,216 .. 10,236) NotI (10,132) FLAG SfiI (8135) SpeI (44) NdeI (279) CMV promoter CMV-F (564 .. 584) p10 promoter PacI (731) FLAG AfeI (846) SbfI (870) PaeR7I - PspXI - XhoI (1127) NsiI (3525) SalI (6647) NheI (7057) BmtI (7061) TspMI - XmaI (7290) SmaI - SrfI (7292) pBacMam2-DiEx-LIC-N entry vector 15,563 bp

Sequence View

SpeIMluISphI 5ʹ 3ʹ 60 CMV enhancer c g g a t c t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a g c c t a g a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t

120 CMV enhancer a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g

180 CMV enhancer g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g

240 CMV enhancer g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a

NdeI 300 CMV enhancer a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a

360 CMV enhancer t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t

420 CMV enhancer CMV promoter c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a

480 CMV promoter t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t

540 CMV promoter c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c

600 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t

660 CMV promoter p10 promoter t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a t a c g g a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t a t g c c

720 p10 promoter a c c t t t a a t t c a a c c c a a c a c a a t a t a t t a t a g t t a a a t a a g a a t t a t t a t c a a a t c a t t t g g a a a t t a a g t t g g g t t g t g t t a t a t a a t a t c a a t t t a t t c t t a a t a a t a g t t t a g t a a

PacI 780 p10 promoter t g t a t a t t a a t t a a a a t a c t a t a c t g t a a a t t a c a t t t t a t t t a c a a t c a c t c g a c g g a t a c a t a t a a t t a a t t t t a t g a t a t g a c a t t t a a t g t a a a a t a a a t g t t a g t g a g c t g c c t a

840 1 5 DYKDDDDK (in frame with FLAG) MATL FLAG c c t t a a g a a g g a g a t a t a c t a t g g a c t a c a a a g a c g a t g a c g a c a a g a t g g c g a c c c t g g g g a a t t c t t c c t c t a t a t g a t a c c t g a t g t t t c t g c t a c t g c t g t t c t a c c g c t g g g a c c

SbfIAfeI 900 EKR* a a a a g c g c t g a c c t t a g t c g c t a a c c t g c a g g a g c c g c t g c a c c g a c c a a a g a a a g a a c t t t t t c g c g a c t g g a a t c a g c g a t t g g a c g t c c t c g g c g a c g t g g c t g g t t t c t t t c t t g a

960 t t c a g c t a c c a a g a a a g a c c g t g t g a a t c a t t g t c t g a c a a t a t g t g a a a a c a t a g t g g c a a g t c g a t g g t t c t t t c t g g c a c a c t t a g t a a c a g a c t g t t a t a c a c t t t t g t a t c a c c g

1020 MELF a c a g t c t g t c a g a a a t t c t c c a g a a t t t c a g a a a c t t c t g g g c a t c g c t a t g g a a c t t t t t g t c a g a c a g t c t t t a a g a g g t c t t a a a g t c t t t g a a g a c c c g t a g c g a t a c c t t g a a a a

1080 LLCSDDAESDVRMVADECLN t c t g c t g t g c a g t g a t g a c g c a g a g t c a g a t g t c a g g a t g g t g g c t g a c g a a t g c c t c a a a g a c g a c a c g t c a c t a c t g c g t c t c a g t c t a c a g t c c t a c c a c c g a c t g c t t a c g g a g t t

PaeR7IPspXIXhoI 1140 KVIKALMDSNLPRLQLELYK c a a a g t t a t c a a a g c t t t g a t g g a t t c t a a t c t t c c a a g g t t a c a g c t c g a g c t c t a t a a g t t t c a a t a g t t t c g a a a c t a c c t a a g a t t a g a a g g t t c c a a t g t c g a g c t c g a g a t a t t

1200 EIKKNGAPRSLRAALWRFAE g g a a a t t a a a a a g a a t g g t g c c c c t c g g a g t t t g c g t g c t g c c c t g t g g a g g t t t g c t g a c c t t t a a t t t t t c t t a c c a c g g g g a g c c t c a a a c g c a c g a c g g g a c a c c t c c a a a c g a c t

1260 LAHLVRPQKCRPYLVNLLPC g c t g g c t c a c c t g g t t c g g c c t c a g a a a t g c a g g c c t t a c c t g g t g a a c c t t c t g c c g t g c g a c c g a g t g g a c c a a g c c g g a g t c t t t a c g t c c g g a a t g g a c c a c t t g g a a g a c g g c a c

1320 LTRTSKRPEESVQETLAAAV c c t g a c t c g a a c a a g c a a g a g a c c c g a a g a a t c a g t c c a g g a g a c c t t g g c t g c a g c t g t g g a c t g a g c t t g t t c g t t c t c t g g g c t t c t t a g t c a g g t c c t c t g g a a c c g a c g t c g a c a

1380 PKIMASFGNFANDNEIKVLL t c c c a a a a t t a t g g c t t c t t t t g g c a a t t t t g c a a a t g a c a a t g a a a t t a a g g t t t t g t t a g g g t t t t a a t a c c g a a g a a a a c c g t t a a a a c g t t t a c t g t t a c t t t a a t t c c a a a a c a a

1440 KAFIANLKSSSPTIRRTAAG a a a g g c c t t c a t a g c g a a c c t g a a g t c a a g c t c c c c c a c c a t t c g g c g g a c a g c g g c t g g t t t c c g g a a g t a t c g c t t g g a c t t c a g t t c g a g g g g g t g g t a a g c c g c c t g t c g c c g a c c

1500 SAVSICQHSRRTQYFYSWLL a t c a g c a g t g a g c a t c t g c c a g c a c t c a a g a a g g a c a c a a t a t t t c t a t a g t t g g c t a c t t a g t c g t c a c t c g t a g a c g g t c g t g a g t t c t t c c t g t g t t a t a a a g a t a t c a a c c g a t g a

1560 NVLLGLLVPVEDEHSTLLIL a a a t g t g c t c t t a g g c t t a c t c g t t c c t g t c g a g g a t g a a c a c t c c a c t c t g c t g a t t c t t t t a c a c g a g a a t c c g a a t g a g c a a g g a c a g c t c c t a c t t g t g a g g t g a g a c g a c t a a g a

1620 GVLLTLRYLVPLLQQQVKDT t g g c g t g c t g c t c a c c c t g a g g t a t t t g g t g c c c t t g c t g c a g c a g c a g g t c a a g g a c a c a c c g c a c g a c g a g t g g g a c t c c a t a a a c c a c g g g a a c g a c g t c g t c g t c c a g t t c c t g t g

1680 SLKGSFGVTRKEMEVSPSAE a a g c c t g a a a g g c a g c t t c g g a g t g a c a a g g a a a g a a a t g g a a g t c t c t c c t t c t g c a g a t t c g g a c t t t c c g t c g a a g c c t c a c t g t t c c t t t c t t t a c c t t c a g a g a g g a a g a c g t c t

1740 QLVQVYELTLHHTQHQDHNV g c a g c t t g t c c a g g t t t a t g a a c t g a c g t t a c a t c a t a c a c a g c a c c a a g a c c a c a a t g t c g t c g a a c a g g t c c a a a t a c t t g a c t g c a a t g t a g t a t g t g t c g t g g t t c t g g t g t t a c a

1800 VTGALELLQQLFRTPPPELL t g t g a c c g g a g c c c t g g a g c t g t t g c a g c a g c t c t t c a g a a c g c c t c c a c c c g a g c t t c t a c a c t g g c c t c g g g a c c t c g a c a a c g t c g t c g a g a a g t c t t g c g g a g g t g g g c t c g a a g a

1860 QTLTAVGGIGQLTAAKEESG g c a a a c c c t g a c c g c a g t c g g g g g c a t t g g g c a g c t c a c c g c t g c t a a g g a g g a g t c t g g c g t t t g g g a c t g g c g t c a g c c c c c g t a a c c c g t c g a g t g g c g a c g a t t c c t c c t c a g a c c

1920 GRSRSGSIVELIAGGGSSCS t g g c c g a a g c c g t a g t g g g a g t a t t g t g g a a c t t a t a g c t g g a g g g g g t t c c t c a t g c a g a c c g g c t t c g g c a t c a c c c t c a t a a c a c c t t g a a t a t c g a c c t c c c c c a a g g a g t a c g t c

1980 PVLSRKQKGKVLLGEEEALE c c c t g t c c t t t c a a g a a a a c a a a a a g g c a a a g t g c t c t t a g g a g a a g a a g a a g c c t t g g a g g g a c a g g a a a g t t c t t t t g t t t t t c c g t t t c a c g a g a a t c c t c t t c t t c t t c g g a a c c t

2040 DDSESRSDVSSSALTASVKD g g a t g a c t c t g a a t c g a g a t c g g a t g t c a g c a g c t c t g c c t t a a c a g c c t c a g t g a a g g a c c t a c t g a g a c t t a g c t c t a g c c t a c a g t c g t c g a g a c g g a a t t g t c g g a g t c a c t t c c t

2100 EISGELAASSGVSTPGSAGH t g a g a t c a g t g g a g a g c t g g c t g c t t c t t c a g g g g t t t c c a c t c c a g g g t c a g c a g g t c a a c t c t a g t c a c c t c t c g a c c g a c g a a g a a g t c c c c a a a g g t g a g g t c c c a g t c g t c c a g t

2160 DIITEQPRSQHTLQADSVDL t g a c a t c a t c a c a g a a c a g c c a c g g t c a c a g c a c a c a c t g c a g g c g g a c t c a g t g g a t c t a c t g t a g t a g t g t c t t g t c g g t g c c a g t g t c g t g t g t g a c g t c c g c c t g a g t c a c c t a g a

2220 ASCDLTSSATDGDEEDILSH g g c c a g c t g t g a c t t g a c a a g c t c t g c c a c t g a t g g g g a t g a g g a g g a t a t c t t g a g c c a c c g g t c g a c a c t g a a c t g t t c g a g a c g g t g a c t a c c c c t a c t c c t c c t a t a g a a c t c g g t

2280 SSSQVSAVPSDPAMDLNDGT c a g c t c c a g c c a g g t c a g c g c c g t c c c a t c t g a c c c t g c c a t g g a c c t g a a t g a t g g g a c g t c g a g g t c g g t c c a g t c g c g g c a g g g t a g a c t g g g a c g g t a c c t g g a c t t a c t a c c c t g

2340 QASSPISDSSQTTTEGPDSA c c a g g c c t c g t c g c c c a t c a g c g a c a g c t c c c a g a c c a c c a c c g a a g g g c c t g a t t c a g c g g t c c g g a g c a g c g g g t a g t c g c t g t c g a g g g t c t g g t g g t g g c t t c c c g g a c t a a g t c g

2400 VTPSDSSEIVLDGTDNQYLG t g t t a c c c c t t c a g a c a g t t c t g a a a t t g t g t t a g a c g g t a c c g a c a a c c a g t a t t t g g g a c a a t g g g g a a g t c t g t c a a g a c t t t a a c a c a a t c t g c c a t g g c t g t t g g t c a t a a a c c c

2460 LQIGQPQDEDEEATGILPDE c c t g c a g a t t g g a c a g c c c c a g g a t g a a g a t g a g g a a g c c a c a g g t a t t c t t c c t g a t g a g g a c g t c t a a c c t g t c g g g g t c c t a c t t c t a c t c c t t c g g t g t c c a t a a g a a g g a c t a c t

2520 ASEAFRNSSMALQQAHLLKN a g c c t c g g a g g c c t t c a g g a a c t c t t c c a t g g c c c t t c a a c a g g c a c a t t t a t t g a a a a a t c g g a g c c t c c g g a a g t c c t t g a g a a g g t a c c g g g a a g t t g t c c g t g t a a a t a a c t t t t t

2580 MSHCRQPSDSSVDKFVLRDE c a t g a g t c a c t g c a g g c a g c c t t c t g a c a g c a g t g t t g a t a a a t t t g t g t t g a g a g a t g a g t a c t c a g t g a c g t c c g t c g g a a g a c t g t c g t c a c a a c t a t t t a a a c a c a a c t c t c t a c t

2640 ATEPGDQENKPCRIKGDIGQ a g c t a c t g a a c c g g g t g a t c a a g a a a a c a a g c c t t g c c g c a t c a a a g g t g a c a t t g g a c a t c g a t g a c t t g g c c c a c t a g t t c t t t t g t t c g g a a c g g c g t a g t t t c c a c t g t a a c c t g t

2700 STDDDSAPLVHCVRLLSASF g t c c a c t g a t g a t g a c t c t g c a c c t c t t g t c c a t t g t g t c c g c c t t t t a t c t g c t t c g t t c a g g t g a c t a c t a c t g a g a c g t g g a g a a c a g g t a a c a c a g g c g g a a a a t a g a c g a a g c a a

2760 LLTGGKNVLVPDRDVRVSVK t t t g c t a a c a g g g g g a a a a a a t g t g c t g g t t c c g g a c a g g g a t g t g a g g g t c a g c g t g a a a a a c g a t t g t c c c c c t t t t t t a c a c g a c c a a g g c c t g t c c c t a c a c t c c c a g t c g c a c t t

2820 ALALSCVGAAVALHPESFFS g g c c c t g g c c c t c a g c t g t g t g g g a g c a g c t g t g g c c c t c c a c c c g g a a t c t t t c t t c a g c c g g g a c c g g g a g t c g a c a c a c c c t c g t c g a c a c c g g g a g g t g g g c c t t a g a a a g a a g t c

2880 KLYKVPLDTTEYPEEQYVSD c a a a c t c t a t a a a g t t c c t c t t g a c a c c a c g g a a t a c c c t g a g g a a c a g t a t g t c t c a g a g t t t g a g a t a t t t c a a g g a g a a c t g t g g t g c c t t a t g g g a c t c c t t g t c a t a c a g a g t c t

2940 ILNYIDHGDPQVRGATAILC c a t c t t g a a c t a c a t c g a t c a t g g a g a c c c a c a g g t t c g a g g a g c c a c t g c c a t t c t c t g g t a g a a c t t g a t g t a g c t a g t a c c t c t g g g t g t c c a a g c t c c t c g g t g a c g g t a a g a g a c

3000 GTLICSILSRSRFHVGDWMG t g g g a c c c t c a t c t g c t c c a t c c t c a g c a g g t c c c g c t t c c a c g t g g g a g a t t g g a t g g g a c c c t g g g a g t a g a c g a g g t a g g a g t c g t c c a g g g c g a a g g t g c a c c c t c t a a c c t a c c c

3060 TIRTLTGNTFSLADCIPLLR c a c c a t t a g a a c c c t c a c a g g a a a t a c a t t t t c t t t g g c g g a t t g c a t t c c t t t g c t g c g g t g g t a a t c t t g g g a g t g t c c t t t a t g t a a a a g a a a c c g c c t a a c g t a a g g a a a c g a c g c

3120 KTLKDESSVTCKLACTAVRN g a a a a c a c t g a a g g a t g a g t c t t c t g t t a c t t g c a a g t t a g c t t g t a c a g c t g t g a g g a a c t t t t g t g a c t t c c t a c t c a g a a g a c a a t g a a c g t t c a a t c g a a c a t g t c g a c a c t c c t t

3180 CVMSLCSSSYSELGLQLIID c t g t g t c a t g a g t c t c t g c a g c a g c a g c t a c a g t g a g t t a g g a c t g c a g c t g a t c a t c g a g a c a c a g t a c t c a g a g a c g t c g t c g t c g a t g t c a c t c a a t c c t g a c g t c g a c t a g t a g c t

3240 VLTLRNSSYWLVRTELLETL t g t g c t g a c t c t g a g g a a c a g t t c c t a t t g g c t g g t g a g g a c a g a g c t t c t g g a a a c c c t a c a c g a c t g a g a c t c c t t g t c a a g g a t a a c c g a c c a c t c c t g t c t c g a a g a c c t t t g g g a

3300 AEIDFRLVSFLEAKAENLHR t g c a g a g a t t g a c t t c a g g c t g g t g a g c t t t t t g g a g g c a a a a g c a g a a a a c t t a c a c a g a c g t c t c t a a c t g a a g t c c g a c c a c t c g a a a a a c c t c c g t t t t c g t c t t t t g a a t g t g t c

3360 GAHHYTGLLKLQERVLNNVV a g g g g c t c a t c a t t a t a c a g g g c t t t t a a a a c t g c a a g a a c g a g t g c t c a a t a a t g t t g t t c c c c g a g t a g t a a t a t g t c c c g a a a a t t t t g a c g t t c t t g c t c a c g a g t t a t t a c a a c a

3420 IHLLGDEDPRVRHVAAASLI c a t c c a t t t g c t t g g a g a t g a a g a c c c c a g g g t g c g a c a t g t t g c c g c a g c a t c a c t a a t g t a g g t a a a c g a a c c t c t a c t t c t g g g g t c c c a c g c t g t a c a a c g g c g t c g t a g t g a t t a

3480 RLVPKLFYKCDQGQADPVVA t a g g c t t g t c c c a a a g c t g t t t t a t a a a t g t g a c c a a g g a c a a g c t g a t c c a g t a g t g g c a t c c g a a c a g g g t t t c g a c a a a a t a t t t a c a c t g g t t c c t g t t c g a c t a g g t c a t c a c c g

NsiI 3540 VARDQSSVYLKLLMHETQPP c g t g g c a a g a g a t c a a a g c a g t g t t t a c c t g a a a c t t c t c a t g c a t g a g a c g c a g c c t c c g c a c c g t t c t c t a g t t t c g t c a c a a a t g g a c t t t g a a g a g t a c g t a c t c t g c g t c g g a g g

3600 SHFSVSTITRIYRGYNLLPS a t c t c a t t t c t c c g t c a g c a c a a t a a c c a g a a t a t a t a g a g g c t a t a a c c t a c t a c c a a g t a g a g t a a a g a g g c a g t c g t g t t a t t g g t c t t a t a t a t c t c c g a t a t t g g a t g a t g g t t c

3660 ITDVTMENNLSRVIAAVSHE c a t a a c a g a c g t c a c t a t g g a a a a t a a c c t t t c a a g a g t t a t t g c a g c a g t t t c t c a t g a g t a t t g t c t g c a g t g a t a c c t t t t a t t g g a a a g t t c t c a a t a a c g t c g t c a a a g a g t a c t

3720 LITSTTRALTFGCCEALCLL a c t a a t c a c a t c a a c c a c c a g a g c a c t c a c a t t t g g a t g c t g t g a a g c t t t g t g t c t t c t t g a t t a g t g t a g t t g g t g g t c t c g t g a g t g t a a a c c t a c g a c a c t t c g a a a c a c a g a a g a

3780 STAFPVCIWSLGWHCGVPPL t t c c a c t g c c t t c c c a g t t t g c a t t t g g a g t t t a g g t t g g c a c t g t g g a g t g c c t c c a c t a a g g t g a c g g a a g g g t c a a a c g t a a a c c t c a a a t c c a a c c g t g a c a c c t c a c g g a g g t g a

3840 SASDESRKSCTVGMATMILT g a g t g c c t c a g a t g a g t c t a g g a a g a g c t g t a c c g t t g g g a t g g c c a c a a t g a t t c t g a c c t c a c g g a g t c t a c t c a g a t c c t t c t c g a c a t g g c a a c c c t a c c g g t g t t a c t a a g a c t g

3900 LLSSAWFPLDLSAHQDALIL c c t g c t c t c g t c a g c t t g g t t c c c a t t g g a t c t c t c a g c c c a t c a a g a t g c t t t g a t t t t g g a c g a g a g c a g t c g a a c c a a g g g t a a c c t a g a g a g t c g g g t a g t t c t a c g a a a c t a a a a

3960 AGNLLAASAPKSLRSSWASE g g c c g g a a a c t t g c t t g c a g c c a g t g c t c c c a a a t c t c t g a g a a g t t c a t g g g c c t c t g a c c g g c c t t t g a a c g a a c g t c g g t c a c g a g g g t t t a g a g a c t c t t c a a g t a c c c g g a g a c t

4020 EEANPAATKQEEVWPALGDR a g a a g a a g c c a a c c c a g c a g c c a c c a a g c a a g a g g a g g t c t g g c c a g c c c t g g g g g a c c g t c t t c t t c g g t t g g g t c g t c g g t g g t t c g t t c t c c t c c a g a c c g g t c g g g a c c c c c t g g c

4080 ALVPMVEQLFSHLLKVINIC g g c c c t g g t g c c c a t g g t g g a g c a g c t c t t c t c t c a c c t g c t g a a g g t g a t t a a c a t t t g c c g g g a c c a c g g g t a c c a c c t c g t c g a g a a g a g a g t g g a c g a c t t c c a c t a a t t g t a a a c

4140 AHVLDDVAPGPAIKAALPSL t g c c c a c g t c c t g g a t g a c g t g g c t c c t g g a c c c g c a a t a a a g g c a g c c t t g c c t t c t c t a c g g g t g c a g g a c c t a c t g c a c c g a g g a c c t g g g c g t t a t t t c c g t c g g a a c g g a a g a g a

4200 TNPPSLSPIRRKGKEKEPGE a a c a a a c c c c c c t t c t c t a a g t c c c a t c c g a c g a a a g g g g a a g g a g a a a g a a c c a g g a g a t t g t t t g g g g g g a a g a g a t t c a g g g t a g g c t g c t t t c c c c t t c c t c t t t c t t g g t c c t c t

4260 QASVPLSPKKGSEASAASRQ a c a a g c a t c t g t a c c g t t g a g t c c c a a g a a a g g c a g t g a g g c c a g t g c a g c t t c t a g a c a t g t t c g t a g a c a t g g c a a c t c a g g g t t c t t t c c g t c a c t c c g g t c a c g t c g a a g a t c t g t

4320 SDTSGPVTTSKSSSLGSFYH a t c t g a t a c c t c a g g t c c t g t t a c a a c a a g t a a a t c c t c a t c a c t g g g g a g t t t c t a t c a t a g a c t a t g g a g t c c a g g a c a a t g t t g t t c a t t t a g g a g t a g t g a c c c c t c a a a g a t a g t

4380 LPSYLKLHDVLKATHANYKV t c t t c c t t c a t a c c t c a a a c t g c a t g a t g t c c t g a a a g c t a c a c a c g c t a a c t a c a a g g t a g a a g g a a g t a t g g a g t t t g a c g t a c t a c a g g a c t t t c g a t g t g t g c g a t t g a t g t t c c a

4440 TLDLQNSTEKFGGFLRSALD c a c g c t g g a t c t t c a g a a c a g c a c g g a a a a g t t t g g a g g g t t t c t t c g c t c a g c c t t g g a g t g c g a c c t a g a a g t c t t g t c g t g c c t t t t c a a a c c t c c c a a a g a a g c g a g t c g g a a c c t

4500 VLSQILELATLQDIGKCVEE t g t t c t t t c t c a g a t a c t a g a g c t g g c c a c a c t g c a g g a c a t t g g g a a g t g t g t t g a a g a a c a a g a a a g a g t c t a t g a t c t c g a c c g g t g t g a c g t c c t g t a a c c c t t c a c a c a a c t t c t

4560 ILGYLKSCFSREPMMATVCV g a t c c t a g g a t a c c t g a a a t c c t g c t t t a g t c g a g a a c c a a t g a t g g c a a c t g t t t g t g t c t a g g a t c c t a t g g a c t t t a g g a c g a a a t c a g c t c t t g g t t a c t a c c g t t g a c a a a c a c a

4620 QQLLKTLFGTNLASQFDGLS t c a a c a a t t g t t g a a g a c t c t c t t t g g c a c a a a c t t g g c c t c c c a g t t t g a t g g c t t a t c a g t t g t t a a c a a c t t c t g a g a g a a a c c g t g t t t g a a c c g g a g g g t c a a a c t a c c g a a t a g

4680 SNPSKSQGRAQRLGSSSVRP t t c c a a c c c c a g c a a g t c a c a a g g c c g a g c a c a g c g c c t t g g c t c c t c c a g t g t g a g g c c a a g g t t g g g g t c g t t c a g t g t t c c g g c t c g t g t c g c g g a a c c g a g g a g g t c a c a c t c c g g

4740 GLYHYCFMAPYTHFTQALAD a g g c t t g t a c c a c t a c t g c t t c a t g g c c c c g t a c a c c c a c t t c a c c c a g g c c c t c g c t g a t c c g a a c a t g g t g a t g a c g a a g t a c c g g g g c a t g t g g g t g a a g t g g g t c c g g g a g c g a c t

4800 ASLRNMVQAEQENDTSGWFD c g c c a g c c t g a g g a a c a t g g t g c a g g c g g a g c a g g a g a a c g a c a c c t c g g g a t g g t t t g a g c g g t c g g a c t c c t t g t a c c a c g t c c g c c t c g t c c t c t t g c t g t g g a g c c c t a c c a a a c t

4860 VLQKVSTQLKTNLTSVTKNR t g t c c t c c a g a a a g t g t c t a c c c a g t t g a a g a c a a a c c t c a c g a g t g t c a c a a a g a a c c g a c a g g a g g t c t t t c a c a g a t g g g t c a a c t t c t g t t t g g a g t g c t c a c a g t g t t t c t t g g c

4920 ADKNAIHNHIRLFEPLVIKA t g c a g a t a a g a a t g c t a t t c a t a a t c a c a t t c g t t t g t t t g a a c c t c t t g t t a t a a a a g c a c g t c t a t t c t t a c g a t a a g t a t t a g t g t a a g c a a a c a a a c t t g g a g a a c a a t a t t t t c g

4980 LKQYTTTTCVQLQKQVLDLL t t t a a a a c a g t a c a c g a c t a c a a c a t g t g t g c a g t t a c a g a a g c a g g t t t t a g a t t t g c t a a a t t t t g t c a t g t g c t g a t g t t g t a c a c a c g t c a a t g t c t t c g t c c a a a a t c t a a a c g a

5040 AQLVQLRVNYCLLDSDQVFI g g c g c a g c t g g t t c a g t t a c g g g t t a a t t a c t g t c t t c t g g a t t c a g a t c a g g t g t t t a t c c g c g t c g a c c a a g t c a a t g c c c a a t t a a t g a c a g a a g a c c t a a g t c t a g t c c a c a a a t a

5100 GFVLKQFEYIEVGQFRESEA t g g c t t t g t a t t g a a a c a g t t t g a a t a c a t t g a a g t g g g c c a g t t c a g g g a a t c a g a g g c a c c g a a a c a t a a c t t t g t c a a a c t t a t g t a a c t t c a c c c g g t c a a g t c c c t t a g t c t c c g

5160 IIPNIFFFLVLLSYERYHSK a a t c a t t c c a a a c a t c t t t t t c t t c t t g g t a t t a c t a t c t t a t g a a c g c t a t c a t t c a a a t t a g t a a g g t t t g t a g a a a a a g a a g a a c c a t a a t g a t a g a a t a c t t g c g a t a g t a a g t t t

5220 QIIGIPKIIQLCDGIMASGR a c a g a t c a t t g g a a t t c c t a a a a t c a t t c a g c t c t g t g a t g g c a t c a t g g c c a g t g g a a g t g t c t a g t a a c c t t a a g g a t t t t a g t a a g t c g a g a c a c t a c c g t a g t a c c g g t c a c c t t c

5280 KAVTHAIPALQPIVHDLFVL g a a g g c t g t g a c a c a t g c c a t a c c g g c t c t g c a g c c c a t a g t c c a c g a c c t c t t t g t a t t c t t c c g a c a c t g t g t a c g g t a t g g c c g a g a c g t c g g g t a t c a g g t g c t g g a g a a a c a t a a

5340 RGTNKADAGKELETQKEVVV a a g a g g a a c a a a t a a a g c t g a t g c a g g a a a a g a g c t t g a a a c c c a a a a a g a g g t g g t g g t t t c t c c t t g t t t a t t t c g a c t a c g t c c t t t t c t c g a a c t t t g g g t t t t t c t c c a c c a c c a

5400 SMLLRLIQYHQVLEMFILVL g t c a a t g t t a c t g a g a c t c a t c c a g t a c c a t c a g g t g t t g g a g a t g t t c a t t c t t g t c c t c a g t t a c a a t g a c t c t g a g t a g g t c a t g g t a g t c c a c a a c c t c t a c a a g t a a g a a c a g g a

5460 QQCHKENEDKWKRLSRQIAD g c a g c a g t g c c a c a a g g a g a a t g a a g a c a a g t g g a a g c g a c t g t c t c g a c a g a t a g c t g a c g t c g t c a c g g t g t t c c t c t t a c t t c t g t t c a c c t t c g c t g a c a g a g c t g t c t a t c g a c t

5520 IILPMLAKQQMHIDSHEALG c a t c a t c c t c c c a a t g t t a g c c a a a c a g c a g a t g c a c a t t g a c t c t c a t g a a g c c c t t g g g t a g t a g g a g g g t t a c a a t c g g t t t g t c g t c t a c g t g t a a c t g a g a g t a c t t c g g g a a c c

5580 VLNTLFEILAPSSLRPVDML a g t g t t a a a t a c a t t a t t t g a g a t t t t g g c c c c t t c c t c c c t c c g t c c g g t a g a c a t g c t t c a c a a t t t a t g t a a t a a a c t c t a a a a c c g g g g a a g g a g g g a g g c a g g c c a t c t g t a c g a

5640 LRSMFVTPNTMASVSTVQLW t t t a c g g a g t a t g t t c g t c a c t c c a a a c a c a a t g g c g t c c g t g a g c a c t g t t c a a c t g t g a a a t g c c t c a t a c a a g c a g t g a g g t t t g t g t t a c c g c a g g c a c t c g t g a c a a g t t g a c a c

5700 ISGILAILRVLISQSTEDIV g a t a t c g g g a a t t c t g g c c a t t t t g a g g g t t c t g a t t t c c c a g t c a a c t g a a g a t a t t g t c t a t a g c c c t t a a g a c c g g t a a a a c t c c c a a g a c t a a a g g g t c a g t t g a c t t c t a t a a c a

5760 LSRIQELSFSPYLISCTVIN t c t t t c t c g t a t t c a g g a g c t c t c c t t c t c t c c g t a t t t a a t c t c c t g t a c a g t a a t t a a a g a a a g a g c a t a a g t c c t c g a g a g g a a g a g a g g c a t a a a t t a g a g g a c a t g t c a t t a a t t

5820 RLRDGDSTSTLEEHSEGKQI t a g g t t a a g a g a t g g g g a c a g t a c t t c a a c g c t a g a a g a a c a c a g t g a a g g g a a a c a a a t a t c c a a t t c t c t a c c c c t g t c a t g a a g t t g c g a t c t t c t t g t g t c a c t t c c c t t t g t t t a

5880 KNLPEETFSRFLLQLVGILL a a a g a a t t t g c c a g a a g a a a c a t t t t c a a g g t t t c t a t t a c a a c t g g t t g g t a t t c t t t t t t t c t t a a a c g g t c t t c t t t g t a a a a g t t c c a a a g a t a a t g t t g a c c a a c c a t a a g a a a a

5940 EDIVTKQLKVEMSEQQHTFY a g a a g a c a t t g t t a c a a a a c a g c t g a a g g t g g a a a t g a g t g a g c a g c a a c a t a c t t t c t a t c t t c t g t a a c a a t g t t t t g t c g a c t t c c a c c t t t a c t c a c t c g t c g t t g t a t g a a a g a t

6000 CQELGTLLMCLIHIFKSGMF t t g c c a g g a a c t a g g c a c a c t g c t a a t g t g t c t g a t c c a c a t c t t c a a g t c t g g a a t g t t a a c g g t c c t t g a t c c g t g t g a c g a t t a c a c a g a c t a g g t g t a g a a g t t c a g a c c t t a c a a

6060 RRITAAATRLFRSDGCGGSF c c g g a g a a t c a c a g c a g c t g c c a c t a g g c t g t t c c g c a g t g a t g g c t g t g g c g g c a g t t t g g c c t c t t a g t g t c g t c g a c g g t g a t c c g a c a a g g c g t c a c t a c c g a c a c c g c c g t c a a a

6120 YTLDSLNLRARSMITTHPAL c t a c a c c c t g g a c a g c t t g a a c t t g c g g g c t c g t t c c a t g a t c a c c a c c c a c c c g g c c c t g a t g t g g g a c c t g t c g a a c t t g a a c g c c c g a g c a a g g t a c t a g t g g t g g g t g g g c c g g g a

6180 VLLWCQILLLVNHTDYRWWA g g t g c t g c t c t g g t g t c a g a t a c t g c t g c t t g t c a a c c a c a c c g a c t a c c g c t g g t g g g c c c a c g a c g a g a c c a c a g t c t a t g a c g a c g a a c a g t t g g t g t g g c t g a t g g c g a c c a c c c g

6240 EVQQTPKRHSLSSTKLLSPQ a g a a g t g c a g c a g a c c c c g a a a a g a c a c a g t c t g t c c a g c a c a a a g t t a c t t a g t c c c c a t c t t c a c g t c g t c t g g g g c t t t t c t g t g t c a g a c a g g t c g t g t t t c a a t g a a t c a g g g g t

6300 MSGEEEDSDLAAKLGMCNRE g a t g t c t g g a g a a g a g g a g g a t t c t g a c t t g g c a g c c a a a c t t g g a a t g t g c a a t a g a g a c t a c a g a c c t c t t c t c c t c c t a a g a c t g a a c c g t c g g t t t g a a c c t t a c a c g t t a t c t c t

6360 IVRRGALILFCDYVCQNLHD a a t a g t a c g a a g a g g g g c t c t c a t t c t c t t c t g t g a t t a t g t c t g t c a g a a c c t c c a t g a t t a t c a t g c t t c t c c c c g a g a g t a a g a g a a g a c a c t a a t a c a g a c a g t c t t g g a g g t a c t

6420 SEHLTWLIVNHIQDLISLSH c t c c g a g c a c t t a a c g t g g c t c a t t g t a a a t c a c a t t c a a g a t c t g a t c a g c c t t t c c c a g a g g c t c g t g a a t t g c a c c g a g t a a c a t t t a g t g t a a g t t c t a g a c t a g t c g g a a a g g g t

6480 EPPVQDFISAVHRNSAASGL c g a g c c t c c a g t a c a g g a c t t c a t c a g t g c c g t t c a t c g g a a c t c t g c t g c c a g c g g c c t g c t c g g a g g t c a t g t c c t g a a g t a g t c a c g g c a a g t a g c c t t g a g a c g a c g g t c g c c g g a

6540 FIQAIQSRCENLSTPTMLKK g t t c a t c c a g g c a a t t c a g t c t c g t t g t g a a a a c c t t t c a a c t c c a a c c a t g c t g a a g a a c a a g t a g g t c c g t t a a g t c a g a g c a a c a c t t t t g g a a a g t t g a g g t t g g t a c g a c t t c t t

6600 TLQCLEGIHLSQSGAVLTLY a a c t c t t c a g t g c t t g g a g g g g a t c c a t c t c a g c c a g t c g g g a g c t g t g c t c a c g c t g t a t t g a g a a g t c a c g a a c c t c c c c t a g g t a g a g t c g g t c a g c c c t c g a c a c g a g t g c g a c a t

SalI 6660 VDRLLCTPFRVLARMVDILA t g t g g a c a g g c t t c t g t g c a c c c c t t t c c g t g t g c t g g c t c g c a t g g t c g a c a t c c t t g c a c a c c t g t c c g a a g a c a c g t g g g g a a a g g c a c a c g a c c g a g c g t a c c a g c t g t a g g a a c g

6720 CRRVEMLLAANLQSSMAQLP t t g t c g c c g g g t a g a a a t g c t t c t g g c t g c a a a t t t a c a g a g c a g c a t g g c c c a g t t g c c a a c a g c g g c c c a t c t t t a c g a a g a c c g a c g t t t a a a t g t c t c g t c g t a c c g g g t c a a c g g

6780 MEELNRIQEYLQSSGLAQRH a a t g g a a g a a c t c a a c a g a a t c c a g g a a t a c c t t c a g a g c a g c g g g c t c g c t c a g a g a c a t t a c c t t c t t g a g t t g t c t t a g g t c c t t a t g g a a g t c t c g t c g c c c g a g c g a g t c t c t g t

6840 QRLYSLLDRFRLSTMQDSLS c c a a a g g c t c t a t t c c c t g c t g g a c a g g t t t c g t c t c t c c a c c a t g c a a g a c t c a c t t a g g g t t t c c g a g a t a a g g g a c g a c c t g t c c a a a g c a g a g a g g t g g t a c g t t c t g a g t g a a t c

6900 PSPPVSSHPLDGDGHVSLET t c c c t c t c c t c c a g t c t c t t c c c a c c c g c t g g a c g g g g a t g g g c a c g t g t c a c t g g a a a c a g g g a g a g g a g g t c a g a g a a g g g t g g g c g a c c t g c c c c t a c c c g t g c a c a g t g a c c t t t g

6960 VSPDKDWYVHLVKSQCWTRS a g t g a g t c c g g a c a a a g a c t g g t a c g t t c a t c t t g t c a a a t c c c a g t g t t g g a c c a g g t c t c a c t c a g g c c t g t t t c t g a c c a t g c a a g t a g a a c a g t t t a g g g t c a c a a c c t g g t c c a g

7020 DSALLEGAELVNRIPAEDMN a g a t t c t g c a c t g c t g g a a g g t g c a g a g c t g g t g a a t c g g a t t c c t g c t g a a g a t a t g a a t c t a a g a c g t g a c g a c c t t c c a c g t c t c g a c c a c t t a g c c t a a g g a c g a c t t c t a t a c t t

BmtINheI 7080 AFMMNSEFNLSLLAPCLSLG *P t g c c t t c a t g a t g a a c t c g g a g t t c a a c c t a a g c c t g c t a g c t c c a t g c t t a a g c c t a g g a c g g a a g t a c t a c t t g a g c c t c a a g t t g g a t t c g g a c g a t c g a g g t a c g a a t t c g g a t c c

7140 MSEISGGQKSALFEAAREVT HTFNRTALLTGKKFCGTLHS g a t g a g t g a a a t t t c t g g t g g c c a g a a g a g t g c c c t t t t t g a a g c a g c c c g t g a g g t g a c c t a c t c a c t t t a a a g a c c a c c g g t c t t c t c a c g g g a a a a a c t t c g t c g g g c a c t c c a c t g

7200 LARVSGTVQQLPAVHHVFQP QGTHAAGHLLERSDMMDELG t c t g g c c c g t g t g a g c g g c a c c g t g c a g c a g c t c c c t g c t g t c c a t c a t g t c t t c c a g c c a g a c c g g g c a c a c t c g c c g t g g c a c g t c g t c g a g g g a c g a c a g g t a g t a c a g a a g g t c g g

7260 ELPAEPAAYWSKLNDLFGDA LQRCFRRGVPALQIIQKPIS c g a g c t g c c t g c a g a a c c g g c g g c c t a c t g g a g c a a g t t g a a t g a t c t g t t t g g g g a t g c g c t c g a c g g a c g t c t t g g c c g c c g g a t g a c c t c g t t c a a c t t a c t a g a c a a a c c c c t a c g

TspMIXmaISmaISrfI 7320 ALYQSLPTLARALAQYLVVV CQILGQGSQGPGQCLVQHHD t g c a c t g t a t c a g t c c c t g c c c a c t c t g g c c c g g g c c c t g g c a c a g t a c c t g g t g g t g g t a c g t g a c a t a g t c a g g g a c g g g t g a g a c c g g g c c c g g g a c c g t g t c a t g g a c c a c c a c c a

7380 SKLPSHLHLPPEKEKDIVKF GFQGTMQVKRRLFLLVNHFE c t c c a a a c t g c c c a g t c a t t t g c a c c t t c c t c c t g a g a a a g a g a a g g a c a t t g t g a a a t t g a g g t t t g a c g g g t c a g t a a a c g t g g a a g g a g g a c t c t t t c t c t t c c t g t a a c a c t t t a a

7440 VVATLEALSWHLIHEQIPLS HHCGKLGQGPMQDMLLDRQT c g t g g t g g c a a c c c t t g a g g c c c t g t c c t g g c a t t t g a t c c a t g a g c a g a t c c c g c t g a g g c a c c a c c g t t g g g a a c t c c g g g a c a g g a c c g t a a a c t a g g t a c t c g t c t a g g g c g a c t c

7500 LDLQAGLDCCCLALQLPGLW QIELCPQVAAAQGQLQRAEP t c t g g a t c t c c a g g c a g g g c t g g a c t g c t g c t g c c t g g c c c t g c a g c t g c c t g g c c t c t g a g a c c t a g a g g t c c g t c c c g a c c t g a c g a c g a c g g a c c g g g a c g t c g a c g g a c c g g a g a c

7560 SVVSSTEFVTHACSLIHCVH AHDGGCLKHGVGAGEDVTHV g a g c g t g g t c t c c t c c a c a g a g t t t g t g a c c c a c g c c t g c t c c c t c a t c c a c t g t g t g c a c t c g c a c c a g a g g a g g t g t c t c a a a c a c t g g g t g c g g a c g a g g g a g t a g g t g a c a c a c g t

7620 FILEAVAVQPGEQLLSPERR EDQLGNCHLRSLLKKTWFSP c t t c a t c c t g g a g g c c g t t g c a g t g c a g c c t g g a g a g c a g c t t c t t a g t c c a g a a a g a a g g a a g t a g g a c c t c c g g c a a c g t c a c g t c g g a c c t c t c g t c g a a g a a t c a g g t c t t t c t t c

7680 TNTPKAISEEEEEVDPNTQN CIGWFGDALLLLFYIWVCLI g a c a a a t a c c c c a a a a g c c a t c a g c g a g g a g g a g g a g g a a g t a g a t c c a a a c a c a c a g a a c t g t t t a t g g g g t t t t c g g t a g t c g c t c c t c c t c c t c c t t c a t c t a g g t t t g t g t g t c t t

7740 PKYITAACEMVAEMVESLQS RLIDSCGTLHHCFHHLRQLR t c c t a a g t a t a t c a c t g c a g c c t g t g a g a t g g t g g c a g a a a t g g t g g a g t c t c t g c a g t c a g g a t t c a t a t a g t g a c g t c g g a c a c t c t a c c a c c g t c t t t a c c a c c t c a g a g a c g t c a g

7800 VLALGHKRNSGVPAFLTPLL HQGQTM g g t g t t g g c c t t g g g t c a t a a a a g g a a t a g c g g c g t g c c g g c g t t t c t c a c g c c a t t g c t c c a c a a c c g g a a c c c a g t a t t t t c c t t a t c g c c g c a c g g c c g c a a a g a g t g c g g t a a c g a

7860 RNIIISLARLPLVNSYTRVP c a g g a a c a t c a t c a t c a g c c t g g c c c g c c t g c c c c t t g t c a a c a g c t a c a c a c g t g t g c c g t c c t t g t a g t a g t a g t c g g a c c g g g c g g a c g g g g a a c a g t t g t c g a t g t g t g c a c a c g g

7920 PLVWKLGWSPKPGGDFGTAF c c c a c t g g t g t g g a a g c t t g g a t g g t c a c c c a a a c c g g g a g g g g a t t t t g g c a c a g c a t t g g g t g a c c a c a c c t t c g a a c c t a c c a g t g g g t t t g g c c c t c c c c t a a a a c c g t g t c g t a a

7980 PEIPVEFLQEKEVFKEFIYR c c c t g a g a t c c c c g t g g a g t t c c t c c a g g a a a a g g a a g t c t t t a a g g a g t t c a t c t a c c g g g g a c t c t a g g g g c a c c t c a a g g a g g t c c t t t t c c t t c a g a a a t t c c t c a a g t a g a t g g c

8040 INTLGWTSRTQFEETWATLL c a t c a a c a c a c t a g g c t g g a c c a g t c g t a c t c a g t t t g a a g a a a c t t g g g c c a c c c t c c t g t a g t t g t g t g a t c c g a c c t g g t c a g c a t g a g t c a a a c t t c t t t g a a c c c g g t g g g a g g a

8100 GVLVTQPLVMEQEESPPEED t g g t g t c c t g g t g a c g c a g c c c c t c g t g a t g g a g c a g g a g g a g a g c c c a c c a g a a g a a g a a c c a c a g g a c c a c t g c g t c g g g g a g c a c t a c c t c g t c c t c c t c t c g g g t g g t c t t c t t c t

SfiI 8160 TERTQINVLAVQAITSLVLS c a c a g a g a g g a c c c a g a t c a a c g t c c t g g c c g t g c a g g c c a t c a c c t c a c t g g t g c t c a g g t g t c t c t c c t g g g t c t a g t t g c a g g a c c g g c a c g t c c g g t a g t g g a g t g a c c a c g a g t c

8220 AMTVPVAGNPAVSCLEQQPR t g c a a t g a c t g t g c c t g t g g c c g g c a a c c c a g c t g t a a g c t g c t t g g a g c a g c a g c c c c g a c g t t a c t g a c a c g g a c a c c g g c c g t t g g g t c g a c a t t c g a c g a a c c t c g t c g t c g g g g c

8280 NKPLKALDTRFGRKLSIIRG g a a c a a g c c t c t g a a a g c t c t c g a c a c c a g g t t t g g g a g g a a g c t g a g c a t t a t c a g a g g c t t g t t c g g a g a c t t t c g a g a g c t g t g g t c c a a a c c c t c c t t c g a c t c g t a a t a g t c t c c

8340 IVEQEIQAMVSKRENIATHH g a t t g t g g a g c a a g a g a t t c a a g c a a t g g t t t c a a a g a g a g a g a a t a t t g c c a c c c a t c a c t a a c a c c t c g t t c t c t a a g t t c g t t a c c a a a g t t t c t c t c t c t t a t a a c g g t g g g t a g t

8400 LYQAWDPVPSLSPATTGALI t t t a t a t c a g g c a t g g g a t c c t g t c c c t t c t c t g t c t c c g g c t a c t a c a g g t g c c c t c a t a a a t a t a g t c c g t a c c c t a g g a c a g g g a a g a g a c a g a g g c c g a t g a t g t c c a c g g g a g t a

8460 SHEKLLLQINPERELGSMSY c a g c c a c g a g a a g c t g c t g c t g c a g a t c a a c c c c g a g c g g g a g c t g g g g a g c a t g a g c t a g t c g g t g c t c t t c g a c g a c g a c g t c t a g t t g g g g c t c g c c c t c g a c c c c t c g t a c t c g a t

8520 KLGQVSIHSVWLGNSITPLR c a a a c t c g g c c a g g t g t c c a t a c a c t c c g t g t g g c t a g g g a a c a g c a t c a c a c c c c t g a g g t t t g a g c c g g t c c a c a g g t a t g t g a g g c a c a c c g a t c c c t t g t c g t a g t g t g g g g a c t c

8580 EEEWDEEEEEEADAPAPSSP g g a g g a g g a a t g g g a c g a g g a a g a g g a g g a g g a g g c c g a c g c c c c t g c a c c t t c g t c a c c c c t c c t c c t t a c c c t g c t c c t t c t c c t c c t c c t c c g g c t g c g g g g a c g t g g a a g c a g t g g

8640 PTSPVNSRKHRAGVDIHSCS a c c c a c g t c t c c a g t c a a c t c c a g g a a a c a c c g g g c t g g a g t t g a c a t c c a c t c c t g t t c t g g g t g c a g a g g t c a g t t g a g g t c c t t t g t g g c c c g a c c t c a a c t g t a g g t g a g g a c a a g

8700 QFLLELYSRWILPSSSARRT g c a g t t t t t g c t t g a g t t g t a c a g c c g c t g g a t c c t g c c g t c c a g c t c a g c c a g g a g g a c c g t c a a a a a c g a a c t c a a c a t g t c g g c g a c c t a g g a c g g c a g g t c g a g t c g g t c c t c c t g

8760 PAILISEVVRSLLVVSDLFT c c c g g c c a t c c t g a t c a g t g a g g t g g t c a g a t c c c t t c t a g t g g t c t c a g a c t t g t t c a c g g g c c g g t a g g a c t a g t c a c t c c a c c a g t c t a g g g a a g a t c a c c a g a g t c t g a a c a a g t g

8820 ERNQFELMYVTLTELRRVHP c g a g c g c a a c c a g t t t g a g c t g a t g t a t g t g a c g c t g a c a g a a c t g c g a a g g g t g c a c c c g c t c g c g t t g g t c a a a c t c g a c t a c a t a c a c t g c g a c t g t c t t g a c g c t t c c c a c g t g g g

8880 SEDEILAQYLVPATCKAAAV t t c a g a a g a c g a g a t c c t c g c t c a g t a c c t g g t g c c t g c c a c c t g c a a g g c a g c t g c c g t a a g t c t t c t g c t c t a g g a g c g a g t c a t g g a c c a c g g a c g g t g g a c g t t c c g t c g a c g g c a

8940 LGMDKAVAEPVSRLLESTLR c c t t g g g a t g g a c a a g g c c g t g g c g g a g c c t g t c a g c c g c c t g c t g g a g a g c a c g c t c a g g g a a c c c t a c c t g t t c c g g c a c c g c c t c g g a c a g t c g g c g g a c g a c c t c t c g t g c g a g t c

9000 SSHLPSRVGALHGILYVLEC g a g c a g c c a c c t g c c c a g c a g g g t t g g a g c c c t g c a c g g c a t c c t c t a t g t g c t g g a g t g c t c g t c g g t g g a c g g g t c g t c c c a a c c t c g g g a c g t g c c g t a g g a g a t a c a c g a c c t c a c

9060 DLLDDTAKQLIPVISDYLLS c g a c c t g c t g g a c g a c a c t g c c a a g c a g c t c a t c c c g g t c a t c a g c g a c t a t c t c c t c t c g c t g g a c g a c c t g c t g t g a c g g t t c g t c g a g t a g g g c c a g t a g t c g c t g a t a g a g g a g a g

9120 NLKGIAHCVNIHSQQHVLVM c a a c c t g a a a g g g a t c g c c c a c t g c g t g a a c a t t c a c a g c c a g c a g c a c g t a c t g g t c a t g t t g g a c t t t c c c t a g c g g g t g a c g c a c t t g t a a g t g t c g g t c g t c g t g c a t g a c c a g t a

9180 CATAFYLIENYPLDVGPEFS g t g t g c c a c t g c g t t t t a c c t c a t t g a g a a c t a t c c t c t g g a c g t a g g g c c g g a a t t t t c c a c a c g g t g a c g c a a a a t g g a g t a a c t c t t g a t a g g a g a c c t g c a t c c c g g c c t t a a a a g

9240 ASIIQMCGVMLSGSEESTPS a g c a t c a a t a a t a c a g a t g t g t g g g g t g a t g c t g t c t g g a a g t g a g g a g t c c a c c c c c t c t c g t a g t t a t t a t g t c t a c a c a c c c c a c t a c g a c a g a c c t t c a c t c c t c a g g t g g g g g a g

9300 IIYHCALRGLERLLLSEQLS c a t c a t t t a c c a c t g t g c c c t c a g a g g c c t g g a g c g c c t c c t g c t c t c t g a g c a g c t c t c g t a g t a a a t g g t g a c a c g g g a g t c t c c g g a c c t c g c g g a g g a c g a g a g a c t c g t c g a g a g

9360 RLDAESLVKLSVDRVNVHSP c c g c c t g g a t g c a g a a t c g c t g g t c a a g c t g a g t g t g g a c a g a g t g a a c g t g c a c a g c c c g g c g g a c c t a c g t c t t a g c g a c c a g t t c g a c t c a c a c c t g t c t c a c t t g c a c g t g t c g g g

9420 Kozak sequence HRAMAALGLMLTCMYTGKEK g c a c c g g g c c a t g g c g g c t c t g g g c c t g a t g c t c a c c t g c a t g t a c a c a g g a a a g g a g a a c g t g g c c c g g t a c c g c c g a g a c c c g g a c t a c g a g t g g a c g t a c a t g t g t c c t t t c c t c t t

9480 VSPGRTSDPNPAAPDSESVI a g t c a g t c c g g g t a g a a c t t c a g a c c c t a a t c c t g c a g c c c c c g a c a g c g a g t c a g t g a t t c a g t c a g g c c c a t c t t g a a g t c t g g g a t t a g g a c g t c g g g g g c t g t c g c t c a g t c a c t a

9540 VAMERVSVLFDRIRKGFPCE t g t t g c t a t g g a g c g g g t a t c t g t t c t t t t t g a t a g g a t c a g g a a a g g c t t t c c t t g t g a a c a a c g a t a c c t c g c c c a t a g a c a a g a a a a a c t a t c c t a g t c c t t t c c g a a a g g a a c a c t

9600 ARVVARILPQFLDDFFPPQD a g c c a g a g t g g t g g c c a g g a t c c t g c c c c a g t t t c t a g a c g a c t t c t t c c c a c c c c a g g a t c g g t c t c a c c a c c g g t c c t a g g a c g g g g t c a a a g a t c t g c t g a a g a a g g g t g g g g t c c t

9660 IMNKVIGEFLSNQQPYPQFM c a t c a t g a a c a a a g t c a t c g g a g a g t t t c t g t c c a a c c a g c a g c c a t a c c c c c a g t t c a t g t a g t a c t t g t t t c a g t a g c c t c t c a a a g a c a g g t t g g t c g t c g g t a t g g g g g t c a a g t a

9720 ATVVYKVFQTLHSTGQSSMV g g c c a c c g t g g t g t a t a a g g t g t t t c a g a c t c t g c a c a g c a c c g g g c a g t c g t c c a t g g t c c g g t g g c a c c a c a t a t t c c a c a a a g t c t g a g a c g t g t c g t g g c c c g t c a g c a g g t a c c a

9780 RDWVMLSLSNFTQRAPVAMA c c g g g a c t g g g t c a t g c t g t c c c t c t c c a a c t t c a c g c a g a g g g c c c c g g t c g c c a t g g c g g c c c t g a c c c a g t a c g a c a g g g a g a g g t t g a a g t g c g t c t c c c g g g g c c a g c g g t a c c g

9840 TWSLSCFFVSASTSPWVAAI c a c g t g g a g c c t c t c c t g c t t c t t t g t c a g c g c g t c c a c c a g c c c g t g g g t c g c g g c g a t g t g c a c c t c g g a g a g g a c g a a g a a a c a g t c g c g c a g g t g g t c g g g c a c c c a g c g c c g c t a

9900 LPHVISRMGKLEQVDVNLFC c c t c c c a c a t g t c a t c a g c a g g a t g g g c a a g c t g g a g c a g g t g g a c g t g a a c c t t t t c t g g g a g g g t g t a c a g t a g t c g t c c t a c c c g t t c g a c c t c g t c c a c c t g c a c t t g g a a a a g a c

9960 LVATDFYRHQIEEELDRRAF c c t g g t c g c c a c a g a c t t c t a c a g a c a c c a g a t a g a g g a g g a g c t c g a c c g c a g g g c c t t g g a c c a g c g g t g t c t g a a g a t g t c t g t g g t c t a t c t c c t c c t c g a g c t g g c g t c c c g g a a

10,020 QSVLEVVAAPGSPYHRLLTC c c a g t c t g t g c t t g a g g t g g t t g c a g c c c c a g g a a g c c c a t a t c a c c g g c t g c t g a c t t g g g t c a g a c a c g a a c t c c a c c a a c g t c g g g g t c c t t c g g g t a t a g t g g c c g a c g a c t g a a c

10,080 1 HHH 10xHis LRNVHKVTTC* t t t a c g a a a t g t c c a c a a g g t c a c c a c c t g c t a a a a c c t c t a c t t c c a a t c g c a c c a t c a a a a t g c t t t a c a g g t g t t c c a g t g g t g g a c g a t t t t g g a g a t g a a g g t t a g c g t g g t a g t

NotI 10,140 5 10 HHHHHHH 1 5 DYKDDDDK * 10xHis FLAG t c a c c a t c a c c a t c a c c a c c a t g a t t a c a a g g a t g a c g a c g a t a a g t g a g g c g g c c g c c a a g t g g t a g t g g t a g t g g t g g t a c t a a t g t t c c t a c t g c t g c t a t t c a c t c c g c c g g c g g t

10,200 a c t t g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g a a c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g

10,260 A C A A C G A G G A A A A T G C G A T A C WPRE-R MPL t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a

10,320 YHAIASRMAFIFSSLYKSWL MLLLPVWLSFSPPCINPG g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a

10,380 LSLYEELWPVVRQRGVVCTV CCLFMRSCGPLSGNVAWCAL g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a

10,440 FADATPTGWGIATTCQLLSG CLLTQPPLVGALPPPVSSFP g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c

10,500 TFAFPLPIATAELIAACLAR GLSLSPSLLPRRNSSPPALP g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c

10,560 * CWTGARLLGTDNSVVLSGKS AAGQGLGCWALTIPWCCRGN c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g

10,620 (in frame with Factor Xa site) RGKRPQEGTNGGPNQAPRGE SSFPWLLACVATWILRGTSF HRPFLGCSPVLPPGFCAGRP a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a

KasINarISfoIPluTI 10,680 AVDR 1 RGEI Factor Xa site CYVPSALNPADLPSRGRPPP SATSLRPSIQRTFLPAGAPL c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g g c g c c c c c c t c c g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c c g c g g g g g g a g g

10,740 HGERSLSRSTRGSLEPAVST LTASEACREVLEDL* t c a c g g c g a g c g a a g c t t g t c g a g a a g t a c t a g a g g a t c t c t a g a g c c t g c a g t c t c g a c a g t g c c g c t c g c t t c g a a c a g c t c t t c a t g a t c t c c t a g a g a t c t c g g a c g t c a g a g c t g

10,800 SLSRSTRGS* a a g c t t g t c g a g a a g t a c t a g a g g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a t t c g a a c a g c t c t t c a t g a t c t c c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t

10,860 c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a

HpaI 10,920 SV40 poly(A) signal C G T T A T C G T A G T G T SV40pA-R g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t

10,980 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev T T A A A G SV40pA-R a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g

11,040 SV40 poly(A) signal Tn7L a a t g t a t c t t a t c a t g t c t g g a t c t g a t c a c t g c t t g a g c c t a g g a g a t c c g a a c c a g a t t t a c a t a g a a t a g t a c a g a c c t a g a c t a g t g a c g a a c t c g g a t c c t c t a g g c t t g g t c t a

11,100 Tn7L a a g t g a a a t c t a g t t c c a a a c t a t t t t g t c a t t t t t a a t t t t c g t a t t a g c t t a c g a c g c t t c a c t t t a g a t c a a g g t t t g a t a a a a c a g t a a a a a t t a a a a g c a t a a t c g a a t g c t g c g

11,160 Tn7L t a c a c c c a g t t c c c a t c t a t t t t g t c a c t c t t c c c t a a a t a a t c c t t a a a a a c t c c a t t t a t g t g g g t c a a g g g t a g a t a a a a c a g t g a g a a g g g a t t t a t t a g g a a t t t t t g a g g t a a a

11,220 Tn7L c c a c c c c t c c c a g t t c c c a a c t a t t t t g t c c g c c c a c a g c g g g g c a t t t t t c t t c c t g t t g g t g g g g a g g g t c a a g g g t t g a t a a a a c a g g c g g g t g t c g c c c c g t a a a a a g a a g g a c a a

11,280 a t g t t t t t a a t c a a a c a t c c t g c c a a c t c c a t g t g a c a a a c c g t c a t c t t c g g c t a c t t t t a c a a a a a t t a g t t t g t a g g a c g g t t g a g g t a c a c t g t t t g g c a g t a g a a g c c g a t g a a a

11,340 t t c t c t g t c a c a g a a t g a a a a t t t t t c t g t c a t c t c t t c g t t a t t a a t g t t t g t a a t t g a a a g a g a c a g t g t c t t a c t t t t a a a a a g a c a g t a g a g a a g c a a t a a t t a c a a a c a t t a a c t

11,400 f1 ori c t g a a t a t c a a c g c t t a t t t g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c g a c t t a t a g t t g c g a a t a a a c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g

11,460 f1 ori a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a

11,520 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c

11,580 f1 ori t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t

11,640 f1 ori c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a

11,700 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a t t a t c a c c t g a g a a c a a g g t t t g a c c t

11,760 f1 ori a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c

11,820 f1 ori g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t

11,880 f1 ori AmpR promoter t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a

11,940 AmpR promoter t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g

12,000 1 5 10 signal sequence MSIQHFRVALI AmpR promoter AmpR MSIQHFRVALI t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g

12,060 15 20 signal sequence PFFAAFCLPVFA 25 30 HPETLVKV AmpR PFFAAFCLPVFAHPETLVKV c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t

12,120 35 40 45 50 KDAEDQLGARVGYIELDLNS AmpR KDAEDQLGARVGYIELDLNS a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c

12,180 55 60 65 70 GKILESFRPEERFPMMSTFK AmpR GKILESFRPEERFPMMSTFK g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c

12,240 75 80 85 90 VLLCGAVLSRIDAGQEQLGR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R VLLCGAVLSRIDAGQEQLGR t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g

12,300 95 100 105 110 RIHYSQNDLVEYSPVTEKHL AmpR RIHYSQNDLVEYSPVTEKHL g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t

12,360 115 120 125 130 TDGMTVRELCSAAITMSDNT AmpR TDGMTVRELCSAAITMSDNT c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c

PvuI 12,420 135 140 145 150 AANLLLTTIGGPKELTAFLH AmpR AANLLLTTIGGPKELTAFLH c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t

12,480 155 160 165 170 NMGDHVTRLDRWEPELNEAI AmpR NMGDHVTRLDRWEPELNEAI a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g

FspI 12,540 175 180 185 190 PNDERDTTMPVAMATTLRKL AmpR PNDERDTTMPVAMATTLRKL c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a

12,600 195 200 205 210 LTGELLTLASRQQLIDWMEA AmpR LTGELLTLASRQQLIDWMEA t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c

12,660 215 220 225 230 DKVAGPLLRSALPAGWFIAD AmpR DKVAGPLLRSALPAGWFIAD a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t

12,720 235 240 245 250 KSGAGERGSRGIIAALGPDG AmpR KSGAGERGSRGIIAALGPDG a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t

12,780 255 260 265 270 KPSRIVVIYTTGSQATMDER AmpR KPSRIVVIYTTGSQATMDER a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t

12,840 275 280 285 NRQIAEIGASLIKHW* AmpR NRQIAEIGASLIKHW* a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c

12,900 t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c

12,960 t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a

13,020 ori g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c

13,080 ori t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g

13,140 ori a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t

13,200 ori c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t

13,260 ori c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g

13,320 ori t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c

13,380 ori g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g

13,440 ori a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c

13,500 ori G G G A A A C G C C T G G T pBR322ori-F t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a

13,560 ori A T C T T T pBR322ori-F a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a

13,620 ori c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c

13,680 c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t

13,740 A L4440 a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t

13,800 G C G A G T C A G T G A G C G A G L4440 g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g

13,860 t g t g c g g t a t t t c a c a c c g c a t a g a c c a g c c g c g t a a c c t g g c a a a a t c g g t t a c g g t t g a c a c g c c a t a a a g t g t g g c g t a t c t g g t c g g c g c a t t g g a c c g t t t t a g c c a a t g c c a a c

13,920 Tn7R a g t a a t a a a t g g a t g c c c t g c g t a a g c g g g t g t g g g c g g a c a a t a a a g t c t t a a a c t g a a t c a t t a t t t a c c t a c g g g a c g c a t t c g c c c a c a c c c g c c t g t t a t t t c a g a a t t t g a c t t

13,980 Tn7R c a a a a t a g a t c t a a a c t a t g a c a a t a a a g t c t t a a a c t a g a c a g a a t a g t t g t a a a c t g a g t t t t a t c t a g a t t t g a t a c t g t t a t t t c a g a a t t t g a t c t g t c t t a t c a a c a t t t g a c t

14,040 Tn7R a a t c a g t c c a g t t a t g c t g t g a a a a a g c a t a c t g g a c t t t t g t t a t g g c t a a a g c a a a c t t t a g t c a g g t c a a t a c g a c a c t t t t t c g t a t g a c c t g a a a a c a a t a c c g a t t t c g t t t g a

14,100 Tn7R c t t c a t t t t c t g a a g t g c a a a t t g c c c g t c g t a t t a a a g a g g g g c g t g g c c a a g g g c a t g g a a g t a a a a g a c t t c a c g t t t a a c g g g c a g c a t a a t t t c t c c c c g c a c c g g t t c c c g t a c

14,160 Tn7R g t a a a g a c t a t a t t c g c g g c g t t g t g a c a a t t t a c c g a a c a a c t c c g c g g c c g g g a a g c c c a t t t c t g a t a t a a g c g c c g c a a c a c t g t t a a a t g g c t t g t t g a g g c g c c g g c c c t t c g g

14,220 175 170 *TATSPDIDFHMV GmR *TATSPDIDFHMV g a t c t c g g c t t g a a c g a a t t g t t a g g t g g c g g t a c t t g g g t c g a t a t c a a a g t g c a t c a c c t a g a g c c g a a c t t g c t t a a c a a t c c a c c g c c a t g a a c c c a g c t a t a g t t t c a c g t a g t g

14,280 165 160 155 150 EERIGLKTYLAVAPDDGYDA GmR EERIGLKTYLAVAPDDGYDA t t c t t c c c g t a t g c c c a a c t t t g t a t a g a g a g c c a c t g c g g g a t c g t c a c c g t a a t c t g c a a g a a g g g c a t a c g g g t t g a a a c a t a t c t c t c g g t g a c g c c c t a g c a g t g g c a t t a g a c g

14,340 145 140 135 130 QVYIVYAGLANAEHKLLNIL GmR QVYIVYAGLANAEHKLLNIL t t g c a c g t a g a t c a c a t a a g c a c c a a g c g c g t t g g c c t c a t g c t t c a a g a g a t t g a t g a g a a c g t g c a t c t a g t g t a t t c g t g g t t c g c g c a a c c g g a g t a c g a a g t t c t c t a a c t a c t c

14,400 125 120 115 110 ATAIGQRRHEGSVALDYIYI GmR ATAIGQRRHEGSVALDYIYI c g c g g t g g c a a t g c c c t g c c t c c g g t g c t c g c c g g a g a c t g c g a g a t c a t a g a t a t a g a t g c g c c a c c g t t a c g g g a c g g a g g c c a c g a g c g g c c t c t g a c g c t c t a g t a t c t a t a t c t a

14,460 105 100 95 90 ESRPQEFKPLVYAALAGVVA GmR ESRPQEFKPLVYAALAGVVA c t c a c t a c g c g g c t g c t c a a a c t t g g g c a g a a c g t a a g c c g c g a g a g c g c c a a c a a c c g c g a g t g a t g c g c c g a c g a g t t t g a a c c c g t c t t g c a t t c g g c g c t c t c g c g g t t g t t g g c g

14,520 85 80 75 70 EQDFAALAIFTKSRLLNGLY GmR EQDFAALAIFTKSRLLNGLY t t c t t g g t c g a a g g c a g c a a g c g c g a t g a a t g t c t t a c t a c g g a g c a a g t t c c c g a g g t a a a g a a c c a g c t t c c g t c g t t c g c g c t a c t t a c a g a a t g a t g c c t c g t t c a a g g g c t c c a t

14,580 65 60 55 50 DSDPQHQSYTAVDGFERGFL GmR DSDPQHQSYTAVDGFERGFL a t c g g a g t c c g g c t g a t g t t g g g a g t a g g t g g c t a c g t c t c c g a a c t c a c g a c c g a a a a g t a g c c t c a g g c c g a c t a c a a c c c t c a t c c a c c g a t g c a g a g g c t t g a g t g c t g g c t t t t c

14,640 45 40 35 30 DLAARMSKVQDPGLRCTRII GmR DLAARMSKVQDPGLRCTRII a t c a a g a g c a g c c c g c a t g g a t t t g a c t t g g t c a g g g c c g a g c c t a c a t g t g c g a a t g a t t a g t t c t c g t c g g g c g t a c c t a a a c t g a a c c a g t c c c g g c t c g g a t g t a c a c g c t t a c t a

14,700 25 20 15 10 GMSSGGLKTKPRSGQQTVDN GmR GMSSGGLKTKPRSGQQTVDN g c c c a t a c t t g a g c c a c c t a a c t t t g t t t t a g g g c g a c t g c c c t g c t g c g t a a c a t c g t t c g g g t a t g a a c t c g g t g g a t t g a a a c a a a a t c c c g c t g a c g g g a c g a c g c a t t g t a g c a a

14,760 5 1 SSRLM GmR SSRLM g c t g c t g c g t a a c a t c g t t g c t g c t c c a t a a c a t c a a a c a t c g a c c c a c g g c g t a a c g c g c g a c g a c g c a t t g t a g c a a c g a c g a g g t a t t g t a g t t t g t a g c t g g g t g c c g c a t t g c g c

14,820 M c t t g c t g c t t g g a t g c c c g a g g c a t a g a c t g t a c a a a a a a a c a g t c a t a a c a a g c c a t g a g a a c g a c g a a c c t a c g g g c t c c g t a t c t g a c a t g t t t t t t t g t c a g t a t t g t t c g g t a c t

14,880 KTATAPLPPLRSVKVLDQLR a a a c c g c c a c t g c g c c g t t a c c a c c g c t g c g t t c g g t c a a g g t t c t g g a c c a g t t g c g t g t t t g g c g g t g a c g c g g c a a t g g t g g c g a c g c a a g c c a g t t c c a a g a c c t g g t c a a c g c a c

14,940 -10 -35 Pc promoter ERIRYLHYSLRTEQAYVNWV a g c g c a t a c g c t a c t t g c a t t a c a g t t t a c g a a c c g a a c a g g c t t a t g t c a a c t g g g t t c t c g c g t a t g c g a t g a a c g t a a t g t c a a a t g c t t g g c t t g t c c g a a t a c a g t t g a c c c a a g

15,000 RAFIRFHGVRHPATLGSSEV g t g c c t t c a t c c g t t t c c a c g g t g t g c g t c a c c c g g c a a c c t t g g g c a g c a g c g a a g t c g c a c g g a a g t a g g c a a a g g t g c c a c a c g c a g t g g g c c g t t g g a a c c c g t c g t c g c t t c a g c

15,060 EAFLSWLANERKVSVSTHRQ a g g c a t t t c t g t c c t g g c t g g c g a a c g a g c g c a a g g t t t c g g t c t c c a c g c a t c g t c a g g t c c g t a a a g a c a g g a c c g a c c g c t t g c t c g c g t t c c a a a g c c a g a g g t g c g t a g c a g t c c

15,120 ALAALLFFYGKVLCTDLPLL c a t t g g c g g c c t t g c t g t t c t t c t a c g g c a a g g t g c t g t g c a c g g a t c t g c c c t t g c t t c g t a a c c g c c g g a a c g a c a a g a a g a t g c c g t t c c a c g a c a c g t g c c t a g a c g g g a a c g a a g

15,180 QEIGRPRPSRRLPVVLTPDE a g g a g a t c g g t a g a c c t c g g c c g t c g c g g c g c t t g c c g g t g g t g c t g a c c c c g g a t g a a g t c c t c t a g c c a t c t g g a g c c g g c a g c g c c g c g a a c g g c c a c c a c g a c t g g g g c c t a c t t c

15,240 VVRILGFLEGEHRLFAQDSS t g g t t c g c a t c c t c g g t t t t c t g g a a g g c g a g c a t c g t t t g t t c g c c c a g g a c t c t a g c t a c c a a g c g t a g g a g c c a a a a g a c c t t c c g c t c g t a g c a a a c a a g c g g g t c c t g a g a t c g a

15,300 YSSSGWLRTRSGYGRACRPD a t a g t t c t a g t g g t t g g c t a c g t a c c c g t a g t g g c t a t g g c a g g g c t t g c c g c c c c g a c g t a t c a a g a t c a c c a a c c g a t g c a t g g g c a t c a c c g a t a c c g t c c c g a a c g g c g g g g c t g c

15,360 VGCEPWAFTRTWGLGWGKGR t t g g c t g c g a g c c c t g g g c c t t c a c c c g a a c t t g g g g g t t g g g g t g g g g a a a a g g a a g a a a a c c g a c g c t c g g g a c c c g g a a g t g g g c t t g a a c c c c c a a c c c c a c c c c t t t t c c t t c t t

15,420 NAGVLVPMGSRWGIDRVPAL a c g c g g g c g t a t t g g t c c c a a t g g g g t c t c g g t g g g g t a t c g a c a g a g t g c c a g c c c t g g t g c g c c c g c a t a a c c a g g g t t a c c c c a g a g c c a c c c c a t a g c t g t c t c a c g g t c g g g a c c

15,480 HSV TK poly(A) signal GPNPAFMNKRPNTRAFYSVF g a c c g a a c c c c g c g t t t a t g a a c a a a c g a c c c a a c a c c c g t g c g t t t t a t t c t g t c t t t t c t g g c t t g g g g c g c a a a t a c t t g t t t g c t g g g t t g t g g g c a c g c a a a a t a a g a c a g a a a a

15,540 HSV TK poly(A) signal T T G T C T C C T T C C G T G T T T C A TK-pA-R LLPS* t a t t g c c g t c a t a g c g c g g g t t c c t t c c g g t a t t g t c t c c t t c c g t g t t t c a g t t a g c c t a t a a c g g c a g t a t c g c g c c c a a g g a a g g c c a t a a c a g a g g a a g g c a c a a a g t c a a t c g g a

3ʹ 5ʹ 15,563 c c c c c a t c t c c c g g t a c c g c a t g g g g g g t a g a g g g c c a t g g c g t a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.