Addgene pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG Sequencing Result


Map View

SgrDI (5981) SspI (5864) Amp-R (5610 .. 5629) ScaI (5540) PvuI (5430) AhdI (5060) pBR322ori-F (4308 .. 4327) PciI (4167) L4440 (4057 .. 4074) CAP binding site M13/pUC Reverse (3839 .. 3861) lac operator M13 Reverse (3826 .. 3842) BstZ17I (3788) EBV-rev (3747 .. 3766) SV40pA-R (3693 .. 3712) PfoI (3591) BstBI (3498) Neo-F (3352 .. 3371) RsrII (3332) BssHII (3213) BglII (12) pRS-marker (44 .. 63) MfeI (161) NruI (208) MluI (228) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) CMV-F (769 .. 789) Eco53kI (816) SacI (818) T7 (863 .. 882) NheI (895) BmtI (899) AflII (908) HindIII (911) EcoNI (1024) BsgI (1216) KflI - PpuMI (1343) BspDI - ClaI (1505) XbaI (1543) PspOMI (1549) ApaI (1553) BGH-rev (1574 .. 1591) F1ori-R (1937 .. 1956) DraIII (2083) F1ori-F (2147 .. 2168) pBABE 3' (2287 .. 2307) CsiI - SexAI * (2373) SV40pro-F (2534 .. 2553) BseRI (2602) StuI (2605) AvrII (2606) TspMI - XmaI (2627) SmaI (2629) Neo-R (2742 .. 2761) KasI (2815) NarI (2816) SfoI (2817) PluTI (2819) PstI (2869) MscI (2898) pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG 5983 bp

Sequence View

BglII 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

MfeI 180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

MluINruI 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

SpeI 300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

660 CMV enhancer CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

780 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

Eco53kISacI 840 CMV promoter G T A G G C G T G CMV-F g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t

BmtINheI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 *YSESYPSGLQSA c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g

AflIIHindIII 960 VF Nluc NLSLSPLGTSNTFLGGPLDE g t t t a a a c t t a a g c t t g g c a a t c c g g t a c t g t t g g t a a a t a a g c c a c c g g g t a a g t c t t c c a a a t t t g a a t t c g a a c c g t t a g g c c a t g a c a a c c a t t t a t t c g g t g g c c c a t t c a g a a g

1020 5 10 15 20 TLEDFVGDWRQTAGYNLDQV Nluc CEFIENPVPSLCGAVVQVLD a c a c t c g a a g a t t t c g t t g g g g a c t g g c g a c a g a c a g c c g g c t a c a a c c t g g a c c a a g t c t g t g a g c t t c t a a a g c a a c c c c t g a c c g c t g t c t g t c g g c c g a t g t t g g a c c t g g t t c a g

EcoNI 1080 25 30 35 40 LEQGGVSSLFQNLGVSVTPI Nluc KFLSTHGTQKLIEPHGYSRD c t t g a a c a g g g a g g t g t g t c c a g t t t g t t t c a g a a t c t c g g g g t g t c c g t a a c t c c g a t c g a a c t t g t c c c t c c a c a c a g g t c a a a c a a a g t c t t a g a g c c c c a c a g g c a t t g a g g c t a g

1140 45 50 55 60 QRIVLSGENGLKIDIHVIIP Nluc LPNDQATFIPQLDVDMDDDR c a a a g g a t t g t c c t g a g c g g t g a a a a t g g g c t g a a g a t c g a c a t c c a t g t c a t c a t c c c g g t t t c c t a a c a g g a c t c g c c a c t t t t a c c c g a c t t c t a g c t g t a g g t a c a g t a g t a g g g c

1200 65 70 75 80 YEGLSGDQMGQIEKIFKVVY Nluc IFTQAAVLHALDFFNKLHHV t a t g a a g g t c t g a g c g g c g a c c a a a t g g g c c a g a t c g a a a a a a t t t t t a a g g t g g t g t a c a t a c t t c c a g a c t c g c c g c t g g t t t a c c c g g t c t a g c t t t t t t a a a a a t t c c a c c a c a t g

BsgI 1260 85 90 95 100 PVDDHHFKVILHYGTLVIDG Nluc RHIIMVKLHDQVIACQYDVP c c t g t g g a t g a t c a t c a c t t t a a g g t g a t c c t g c a c t a t g g c a c a c t g g t a a t c g a c g g g g g a c a c c t a c t a g t a g t g a a a t t c c a c t a g g a c g t g a t a c c g t g t g a c c a t t a g c t g c c c

1320 105 110 115 120 VTPNMIDYFGRPYEGIAVFD Nluc NRRVHDVIESPRIFADGHEV g t t a c g c c g a a c a t g a t c g a c t a t t t c g g a c g g c c g t a t g a a g g c a t c g c c g t g t t c g a c c a a t g c g g c t t g t a c t a g c t g a t a a a g c c t g c c g g c a t a c t t c c g t a g c g g c a c a a g c t g

PpuMIKflI 1380 125 130 135 140 GKKITVTGTLWNGNKIIDER Nluc AFLDSYCPGQPVAVFNDVLA g g c a a a a a g a t c a c t g t a a c a g g g a c c c t g t g g a a c g g c a a c a a a a t t a t c g a c g a g c g c c c g t t t t t c t a g t g a c a t t g t c c c t g g g a c a c c t t g c c g t t g t t t t a a t a g c t g c t c g c g

1440 145 150 155 160 LINPDGSLLFRVTINGVTGW Nluc QDVGVAGQQESYGDVSHGAP c t g a t c a a c c c c g a c g g c t c c c t g c t g t t c c g a g t a a c c a t c a a c g g a g t g a c c g g c t g g g a c t a g t t g g g g c t g c c g a g g g a c g a c a a g g c t c a t t g g t a g t t g c c t c a c t g g c c g a c c

1500 165 170 RLCERILA 1 5 10 DYKDHDGDYKDH Nluc 3xFLAG PQAFANQRVVFVMVTIIFIM c g g c t g t g c g a a c g c a t t c t g g c g g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t c a t g c c g a c a c g c t t g c g t a a g a c c g c c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a g t a

XbaIPspOMIApaIBspDIClaI 1560 15 20 DIDYKDDDDK * 3xFLAG g a c a t c g a t t a c a a g g a t g a c g a t g a c a a g t a a g g c c g c g a c t c t a g a g g g c c c g t t t a a c t g t a g c t a a t g t t c c t a c t g c t a c t g t t c a t t c c g g c g c t g a g a t c t c c c g g g c a a a t t

1620 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g

1680 bGH poly(A) signal c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c

1740 bGH poly(A) signal g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c

1800 bGH poly(A) signal g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a

1860 bGH poly(A) signal f1 ori a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a

1920 f1 ori a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g

1980 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g

2040 f1 ori t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c

DraIII 2100 f1 ori c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t

2160 f1 ori G T G G A C T C T T G T T C F1ori-F t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g

2220 f1 ori C A A A C T G G F1ori-F c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c

2280 f1 ori c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t

2340 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t

SexAI*CsiI 2400 SV40 promoter g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g

2460 SV40 promoter c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g

2520 SV40 promoter SV40 ori t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a

2580 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t

TspMIXmaISmaIBseRIStuIAvrII 2640 SV40 promoter SV40 ori a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t

2700 1 MIEQ NeoR/KanR MIEQ t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c

2760 5 10 15 20 DGLHAGSPAAWVERLFGYDW NeoR/KanR G A T A A G C C G A T A C T G A C C C Neo-R DGLHAGSPAAWVERLFGYDW a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c

KasINarISfoIPluTI 2820 25 30 35 40 AQQTIGCSDAAVFRLSAQGR NeoR/KanR G Neo-R AQQTIGCSDAAVFRLSAQGR c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g

PstI 2880 45 50 55 60 PVLFVKTDLSGALNELQDEA NeoR/KanR PVLFVKTDLSGALNELQDEA c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c

MscI 2940 65 70 75 80 ARLSWLATTGVPCAAVLDVV NeoR/KanR ARLSWLATTGVPCAAVLDVV c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t

3000 85 90 95 100 TEAGRDWLLLGEVPGQDLLS NeoR/KanR TEAGRDWLLLGEVPGQDLLS * c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a

3060 105 110 115 120 SHLAPAEKVSIMADAMRRLH NeoR/KanR SHLAPAEKVSIMADAMRRLH RVKSRGLFYGDHSICHPPQM c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t

3120 125 130 135 140 TLDPATCPFDHQAKHRIERA NeoR/KanR TLDPATCPFDHQAKHRIERA RKIRSGAWEVVLRFMADLSC c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g

3180 145 150 155 160 RTRMEAGLVDQDDLDEEHQG NeoR/KanR RTRMEAGLVDQDDLDEEHQG TSPHFGTKDILIIQVFLMLP g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g

BssHII 3240 165 170 175 180 LAPAELFARLKARMPDGEDL NeoR/KanR LAPAELFARLKARMPDGEDL ERWGFQEGPELRAHGVALIE t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c

3300 185 190 195 200 VVTHGDACLPNIMVENGRFS NeoR/KanR VVTHGDACLPNIMVENGRFS DHGMAIGAQRIDHHFIAAKR t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c

RsrII 3360 205 210 215 220 GFIDCGRLGVADRYQDIALA NeoR/KanR C G T T G G C T A Neo-F GFIDCGRLGVADRYQDIALA SEDVTAPQTHRVAILVYRQS g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t

3420 225 230 235 240 TRDIAEELGGEWADRFLVLY NeoR/KanR C C C G T G A T A T T Neo-F TRDIAEELGGEWADRFLVLY GTINSFLKAAFPSVAEEHKV c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c

3480 245 250 255 260 GIAAPDSQRIAFYRLLDEFF NeoR/KanR GIAAPDSQRIAFYRLLDEFF TDGSGIRLADGEIAKKVLEE g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a

BstBI 3540 265 * NeoR/KanR * SRSEPTRFSRGLSAWGAM g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t

PfoI 3600 t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g

3660 SV40 poly(A) signal c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a

3720 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t

3780 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t

BstZ17I 3840 M13 rev C A G T A T C G A C A A A G G M13 Reverse G G M13/pUC Reverse t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g

3900 -10 M13 rev lac operator lac promoter A C M13 Reverse A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c

3960 -35 lac promoter CAP binding site t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g

4020 c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c

4080 G A G C G A G T G A C T G A G C G A L4440 g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g

4140 g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g

PciI 4200 a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t

4260 ori c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t

4320 ori T T T C T A T G G T C C G pBR322ori-F c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g

4380 ori C A A A G G G pBR322ori-F g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t

4440 ori c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t

4500 ori t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t

4560 ori g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t

4620 ori c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c

4680 ori t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c

4740 ori t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c

4800 ori c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c

4860 ori a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t

4920 c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a

4980 c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g

5040 285 280 275 270 *WHKILSAGIEAIQRNRE AmpR *WHKILSAGIEAIQRNRE t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g

AhdI 5100 265 260 255 250 DMTAQSGTTYIVVIRSPKGD AmpR DMTAQSGTTYIVVIRSPKGD a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g

5160 245 240 235 230 PGLAAIIGRSGREGAGSKDA AmpR PGLAAIIGRSGREGAGSKDA t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g

5220 225 220 215 210 IFWGAPLASRLLPGAVKDAE AmpR IFWGAPLASRLLPGAVKDAE a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g

5280 205 200 195 190 MWDILQQRSALTLLEGTLLK AmpR MWDILQQRSALTLLEGTLLK c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a

5340 185 180 175 170 RLTTAMAVPMTTDREDNPIA AmpR RLTTAMAVPMTTDREDNPIA g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g

5400 165 160 155 150 ENLEPEWRDLRTVHDGMNHL AmpR ENLEPEWRDLRTVHDGMNHL t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t

PvuI 5460 145 140 135 130 FATLEKPGGITTLLLNAATN AmpR FATLEKPGGITTLLLNAATN a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a

5520 125 120 115 110 DSMTIAASCLERVTMGDTLH AmpR DSMTIAASCLERVTMGDTLH a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c

ScaI 5580 105 100 95 90 KETVPSYEVLDNQSYHIRRG AmpR KETVPSYEVLDNQSYHIRRG c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g

5640 85 80 75 70 LQEQGADIRSLVAGCLLVKF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R LQEQGADIRSLVAGCLLVKF g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t

5700 65 60 55 50 TSMMPFREEPRFSELIKGSN AmpR TSMMPFREEPRFSELIKGSN a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a

5760 45 40 35 30 LDLEIYGVRAGLQDEADKVK AmpR LDLEIYGVRAGLQDEADKVK g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a

5820 25 VLTEPH 20 15 10 signal sequence AFVPLCFAAFFPIL AmpR VLTEPHAFVPLCFAAFFPIL c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c

SspI 5880 5 1 signal sequence AVRFHQISM AmpR AmpR promoter AVRFHQISM g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t

5940 AmpR promoter t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a

SgrDI 3ʹ 5ʹ 5983 AmpR promoter a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.