Addgene ADRB2-Tango Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: ADRB2-Tango Sequencing Result


Map View

SgrDI (0) Amp-R (7503 .. 7522) ScaI (7433) PvuI (7323) AhdI (6953) pBR322ori-F (6201 .. 6220) L4440 (5950 .. 5967) CAP binding site M13/pUC Reverse (5732 .. 5754) lac operator M13 Reverse (5719 .. 5735) BstZ17I (5681) EBV-rev (5640 .. 5659) SV40pA-R (5586 .. 5605) BstBI (5391) Neo-F (5245 .. 5264) RsrII (5225) PluTI (4712) SfoI (4710) NarI (4709) KasI (4708) Neo-R (4635 .. 4654) AvrII (4499) StuI (4498) SV40pro-F (4427 .. 4446) CsiI - SexAI * (4266) pBABE 3' (4180 .. 4200) F1ori-F (4040 .. 4061) pRS-marker (46 .. 65) MluI (230) CMV enhancer CMV-F (771 .. 791) T7 (865 .. 884) NheI (897) BmtI (901) AflII (910) Acc65I (919) KpnI (923) BamHI (931) EcoRV (966) NotI (981) BstEII (1272) PmlI (2175) KflI (2347) Tet-R (2501 .. 2520) AfeI (2832) AleI (2966) MauBI (3059) BsiWI (3064) SacII (3173) PshAI (3211) XbaI (3436) PspOMI (3442) ApaI (3446) BGH-rev (3467 .. 3484) F1ori-R (3830 .. 3849) ADRB2-Tango 7874 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T pRS-marker t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t

120 A C G G C pRS-marker t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c

180 c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a

MluI 240 CMV enhancer g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t

300 CMV enhancer t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t

360 CMV enhancer t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t

420 CMV enhancer c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a

480 CMV enhancer c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a

540 CMV enhancer g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t

600 CMV enhancer t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a

660 CMV enhancer CMV promoter c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a

720 CMV promoter t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t

780 CMV promoter C G C A A A T G G G CMV-F c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c

840 CMV promoter C G G T A G G C G T G CMV-F c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g

NheI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t

BamHIAcc65IKpnIAflIIBmtI 960 g c g t t t a a a c t t a a g c t t g g t a c c g a g c t c g g a t c c a c t a g t c c a g t g t g g t g g a a t t c t c g c a a a t t t g a a t t c g a a c c a t g g c t c g a g c c t a g g t g a t c a g g t c a c a c c a c c t t a a g a

NotIEcoRV 1020 MKTIIALSY g c a g a t a t c c a g c a c a g t g g c g g c c g c g c c a c c a t g a a g a c g a t c a t c g c c c t g a g c t a c c g t c t a t a g g t c g t g t c a c c g c c g g c g c g g t g g t a c t t c t g c t a g t a g c g g g a c t c g a t g

1080 IFCLVFADYKDDDDASIDMG a t c t t c t g c c t g g t a t t c g c c g a c t a c a a g g a c g a t g a t g a c g c c a g c a t c g a t a t g g g g t a g a a g a c g g a c c a t a a g c g g c t g a t g t t c c t g c t a c t a c t g c g g t c g t a g c t a t a c c c c

1140 QPGNGSAFLLAPNRSHAPDH c a a c c t g g c a a t g g a t c a g c t t t t c t c c t c g c c c c t a a t c g g a g c c a c g c t c c c g a c c a c g t t g g a c c g t t a c c t a g t c g a a a a g a g g a g c g g g g a t t a g c c t c g g t g c g a g g g c t g g t g

1200 DVTQQRDEVWVVGMGIVMSL g a t g t g a c t c a g c a g a g a g a c g a g g t c t g g g t t g t a g g c a t g g g t a t t g t g a t g t c a c t g c t a c a c t g a g t c g t c t c t c t g c t c c a g a c c c a a c a t c c g t a c c c a t a a c a c t a c a g t g a c

1260 IVLAIVFGNVLVITAIAKFE a t c g t c c t g g c a a t t g t g t t c g g c a a c g t c c t c g t c a t t a c t g c t a t t g c a a a g t t c g a g t a g c a g g a c c g t t a a c a c a a g c c g t t g c a g g a g c a g t a a t g a c g a t a a c g t t t c a a g c t c

BstEII 1320 RLQTVTNYFITSLACADLVM c g g c t t c a g a c g g t t a c c a a t t a c t t t a t t a c t t c c c t c g c c t g t g c c g a c t t g g t c a t g g c c g a a g t c t g c c a a t g g t t a a t g a a a t a a t g a a g g g a g c g g a c a c g g c t g a a c c a g t a c

1380 GLAVVPFGAAHILMKMWTFG g g c c t t g c g g t t g t a c c g t t t g g c g c a g c t c a t a t c c t g a t g a a g a t g t g g a c t t t t g g t c c g g a a c g c c a a c a t g g c a a a c c g c g t c g a g t a t a g g a c t a c t t c t a c a c c t g a a a a c c a

1440 NFWCEFWTSIDVLCVTASIE a a t t t t t g g t g c g a a t t c t g g a c t t c a a t t g a c g t g c t c t g c g t c a c a g c g t c a a t c g a a t t a a a a a c c a c g c t t a a g a c c t g a a g t t a a c t g c a c g a g a c g c a g t g t c g c a g t t a g c t t

1500 TLCVIAVDRYFAITSPFKYQ a c t c t g t g t g t t a t t g c c g t t g a t a g g t a c t t c g c g a t t a c g a g t c c c t t t a a a t a c c a g t g a g a c a c a c a a t a a c g g c a a c t a t c c a t g a a g c g c t a a t g c t c a g g g a a a t t t a t g g t c

1560 SLLTKNKARVIILMVWIVSG t c t c t g c t g a c t a a g a a c a a a g c t c g g g t t a t c a t a c t c a t g g t g t g g a t c g t c t c a g g a a g a g a c g a c t g a t t c t t g t t t c g a g c c c a a t a g t a t g a g t a c c a c a c c t a g c a g a g t c c t

1620 LTSFLPIQMHWYRATHQEAI c t c a c a a g c t t t c t g c c t a t a c a g a t g c a c t g g t a t c g c g c c a c a c a c c a a g a a g c t a t c g a g t g t t c g a a a g a c g g a t a t g t c t a c g t g a c c a t a g c g c g g t g t g t g g t t c t t c g a t a g

1680 NCYANETCCDFFTNQAYAIA a a t t g t t a c g c a a a c g a a a c c t g t t g c g a c t t c t t c a c t a a t c a a g c c t a t g c t a t c g c t t t a a c a a t g c g t t t g c t t t g g a c a a c g c t g a a g a a g t g a t t a g t t c g g a t a c g a t a g c g a

1740 SSIVSFYVPLVIMVFVYSRV a g t t c a a t t g t c t c t t t t t a t g t g c c t c t g g t t a t c a t g g t g t t c g t c t a c t c t c g g g t t t c a a g t t a a c a g a g a a a a a t a c a c g g a g a c c a a t a g t a c c a c a a g c a g a t g a g a g c c c a a

1800 FQEAKRQLQKIDKSEGRFHV t t t c a g g a g g c a a a a a g a c a g c t c c a g a a a a t c g a c a a g t c a g a a g g c c g a t t t c a c g t t a a a g t c c t c c g t t t t t c t g t c g a g g t c t t t t a g c t g t t c a g t c t t c c g g c t a a a g t g c a a

1860 QNLSQVEQDGRTGHGLRRSS c a a a a t c t g a g c c a g g t c g a a c a a g a c g g a a g a a c t g g a c a t g g t c t t c g g a g a t c t t c c g t t t t a g a c t c g g t c c a g c t t g t t c t g c c t t c t t g a c c t g t a c c a g a a g c c t c t a g a a g g

1920 KFCLKEHKALKTLGIIMGTF a a a t t t t g c t t g a a g g a a c a c a a g g c c c t g a a g a c c t t g g g g a t a a t t a t g g g c a c a t t c t t t a a a a c g a a c t t c c t t g t g t t c c g g g a c t t c t g g a a c c c c t a t t a a t a c c c g t g t a a g

1980 TLCWLPFFIVNIVHVIQDNL a c t c t c t g c t g g c t c c c c t t t t t c a t t g t c a a t a t c g t t c a c g t t a t a c a a g a c a a c t t g t g a g a g a c g a c c g a g g g g a a a a a g t a a c a g t t a t a g c a a g t g c a a t a t g t t c t g t t g a a c

2040 IRKEVYILLNWIGYVNSGFN a t c a g a a a a g a g g t g t a t a t c c t c c t g a a c t g g a t t g g c t a c g t g a a c t c t g g g t t c a a c t a g t c t t t t c t c c a c a t a t a g g a g g a c t t g a c c t a a c c g a t g c a c t t g a g a c c c a a g t t g

2100 PLIYCRSPDFRIAFQELLCL c c t c t g a t t t a c t g c c g c a g c c c a g a c t t c a g a a t c g c a t t c c a g g a a t t g c t g t g t c t g g g a g a c t a a a t g a c g g c g t c g g g t c t g a a g t c t t a g c g t a a g g t c c t t a a c g a c a c a g a c

2160 RRSSLKAYGNGYSSNGNTGE *PFPYEELPLVPS a g g c g c t c t t c c t t g a a g g c t t a t g g a a a c g g a t a c t c c t c t a a t g g c a a c a c g g g c g a a t c c g c g a g a a g g a a c t t c c g a a t a c c t t t g c c t a t g a g g a g a t t a c c g t t g t g c c c g c t t

PmlI 2220 QSGYHVEQEKENKLLCEDLP CDPYWTSCSFSFLSNHSSRG c a g t c a g g a t a c c a c g t g g a g c a g g a a a a a g a g a a t a a g c t g t t g t g c g a g g a c c t c c c t g t c a g t c c t a t g g t g c a c c t c g t c c t t t t t c t c t t a t t c g a c a a c a c g c t c c t g g a g g g a

2280 GTEDFVGHQGTVPSDNIDSQ PVSSKTPWWPVTGLSLISLW g g c a c t g a g g a t t t t g t t g g c c a c c a a g g a a c a g t c c c a a g c g a c a a t a t t g a c a g c c a g c c g t g a c t c c t a a a a c a a c c g g t g g t t c c t t g t c a g g g t t c g c t g t t a t a a c t g t c g g t c

2340 GRNCSTNDSLLIDTGGRTPP PRFQLVLSESSISVPPRVGG g g g c g a a a c t g c a g c a c t a a t g a t t c a c t g c t g a t c g a t a c c g g t g g a c g c a c c c c a c c c c c c g c t t t g a c g t c g t g a t t a c t a a g t g a c g a c t a g c t a t g g c c a c c t g c g t g g g g t g g g

KflI 2400 SLGPQDESCTTASSSLAKDT LRPGWSSDQVVALEERALSV a g c c t g g g t c c c c a a g a t g a g t c c t g c a c c a c c g c c a g c t c c t c c c t g g c c a a g g a c a c t t c g g a c c c a g g g g t t c t a c t c a g g a c g t g g t g g c g g t c g a g g a g g g a c c g g t t c c t g t g a

2460 5 10 TetR RLDKSKVIN tTA SSTGENLYFQLRLDKSKVIN EDVPSFRYKWSLNSLLLTIL t c a t c g a c c g g t g a g a a c c t g t a c t t c c a g c t a a g a t t a g a t a a a a g t a a a g t g a t t a a c a g t a g c t g g c c a c t c t t g g a c a t g a a g g t c g a t t c t a a t c t a t t t t c a t t t c a c t a a t t g

2520 15 20 25 30 TetR SALELLNEVGIEGLTTRKLA tTA A T T G T T G G G C A T T T G A G C G G Tet-R SALELLNEVGIEGLTTRKLA LANSSSLSTPISPKVVRLSA a g c g c a t t a g a g c t g c t t a a t g a g g t c g g a a t c g a a g g t t t a a c a a c c c g t a a a c t c g c c t c g c g t a a t c t c g a c g a a t t a c t c c a g c c t t a g c t t c c a a a t t g t t g g g c a t t t g a g c g g

2580 35 40 45 50 TetR QKLGVEQPTLYWHVKNKRAL tTA QKLGVEQPTLYWHVKNKRAL WFSPTSCGVNYQCTFFLRAK c a g a a g c t a g g t g t a g a g c a g c c t a c a t t g t a t t g g c a t g t a a a a a a t a a g c g g g c t t t g g t c t t c g a t c c a c a t c t c g t c g g a t g t a a c a t a a c c g t a c a t t t t t t a t t c g c c c g a a a c

2640 55 60 65 70 TetR LDALAIEMLDRHHTHFCPLE tTA LDALAIEMLDRHHTHFCPLE SSAKAM c t c g a c g c c t t a g c c a t t g a g a t g t t a g a t a g g c a c c a t a c t c a c t t t t g c c c t t t a g a a g a g c t g c g g a a t c g g t a a c t c t a c a a t c t a t c c g t g g t a t g a g t g a a a a c g g g a a a t c t t

2700 75 80 85 90 TetR GESWQDFLRNNAKSFRCALL tTA GESWQDFLRNNAKSFRCALL g g g g a a a g c t g g c a a g a t t t t t t a c g t a a t a a c g c t a a a a g t t t t a g a t g t g c t t t a c t a c c c c t t t c g a c c g t t c t a a a a a a t g c a t t a t t g c g a t t t t c a a a a t c t a c a c g a a a t g a t

2760 95 100 105 110 TetR SHRDGAKVHLGTRPTEKQYE tTA SHRDGAKVHLGTRPTEKQYE a g t c a t c g c g a t g g a g c a a a a g t a c a t t t a g g t a c a c g g c c t a c a g a a a a a c a g t a t g a a t c a g t a g c g c t a c c t c g t t t t c a t g t a a a t c c a t g t g c c g g a t g t c t t t t t g t c a t a c t t

2820 115 120 125 130 TetR TLENQLAFLCQQGFSLENAL tTA TLENQLAFLCQQGFSLENAL a c t c t c g a a a a t c a a t t a g c c t t t t t a t g c c a a c a a g g t t t t t c a c t a g a g a a t g c a t t a t g a g a g c t t t t a g t t a a t c g g a a a a a t a c g g t t g t t c c a a a a a g t g a t c t c t t a c g t a a t

AfeI 2880 135 140 145 150 TetR YALSAVGHFTLGCVLEDQEH tTA YALSAVGHFTLGCVLEDQEH t a t g c a c t c a g c g c t g t g g g g c a t t t t a c t t t a g g t t g c g t a t t g g a a g a t c a a g a g c a t a t a c g t g a g t c g c g a c a c c c c g t a a a a t g a a a t c c a a c g c a t a a c c t t c t a g t t c t c g t a

2940 155 160 165 170 TetR QVAKEERETPTTDSMPPLLR tTA QVAKEERETPTTDSMPPLLR c a a g t c g c t a a a g a a g a a a g g g a a a c a c c t a c t a c t g a t a g t a t g c c g c c a t t a t t a c g a g t t c a g c g a t t t c t t c t t t c c c t t t g t g g a t g a t g a c t a t c a t a c g g c g g t a a t a a t g c t

AleI 3000 175 180 185 190 TetR QAIELFDHQGAEPAFLFGLE tTA QAIELFDHQGAEPAFLFGLE c a a g c t a t c g a a t t a t t t g a t c a c c a a g g t g c a g a g c c a g c c t t c t t a t t c g g c c t t g a a g t t c g a t a g c t t a a t a a a c t a g t g g t t c c a c g t c t c g g t c g g a a g a a t a a g c c g g a a c t t

MauBI 3060 195 200 205 TetR LIICGLEKQLKCESGS 210 VP16 activation domain AYSR tTA LIICGLEKQLKCESGSAYSR t t g a t c a t a t g c g g a t t a g a a a a a c a a c t t a a a t g t g a a a g t g g g t c c g c g t a c a g c c g c a a c t a g t a t a c g c c t a a t c t t t t t g t t g a a t t t a c a c t t t c a c c c a g g c g c a t g t c g g c g

BsiWI 3120 215 220 225 230 VP16 activation domain ARTKNNYGSTIEGLLDLPDD tTA ARTKNNYGSTIEGLLDLPDD g c g c g t a c g a a a a a c a a t t a c g g g t c t a c c a t c g a g g g c c t g c t c g a t c t c c c g g a c g a c c g c g c a t g c t t t t t g t t a a t g c c c a g a t g g t a g c t c c c g g a c g a g c t a g a g g g c c t g c t g

SacII 3180 235 240 245 250 VP16 activation domain DAPEEAGLAAPRLSFLPAGH tTA DAPEEAGLAAPRLSFLPAGH g a c g c c c c c g a a g a g g c g g g g c t g g c g g c t c c g c g c c t g t c c t t t c t c c c c g c g g g a c a c c t g c g g g g g c t t c t c c g c c c c g a c c g c c g a g g c g c g g a c a g g a a a g a g g g g c g c c c t g t g

PshAI 3240 255 260 265 270 VP16 activation domain TRRLSTAPPTDVSLGDELHL tTA TRRLSTAPPTDVSLGDELHL a c g c g c a g a c t g t c g a c g g c c c c c c c g a c c g a t g t c a g c c t g g g g g a c g a g c t c c a c t t a t g c g c g t c t g a c a g c t g c c g g g g g g g c t g g c t a c a g t c g g a c c c c c t g c t c g a g g t g a a t

3300 275 280 285 290 VP16 activation domain DGEDVAMAHADALDDFDLDM tTA DGEDVAMAHADALDDFDLDM g a c g g c g a g g a c g t g g c g a t g g c g c a t g c c g a c g c g c t a g a c g a t t t c g a t c t g g a c a t g c t g c c g c t c c t g c a c c g c t a c c g c g t a c g g c t g c g c g a t c t g c t a a a g c t a g a c c t g t a c

3360 295 300 305 310 VP16 activation domain LGDGDSPGPGFTPHDSAPYG tTA LGDGDSPGPGFTPHDSAPYG t t g g g g g a c g g g g a t t c c c c g g g t c c g g g a t t t a c c c c c c a c g a c t c c g c c c c c t a c g g c a a c c c c c t g c c c c t a a g g g g c c c a g g c c c t a a a t g g g g g g t g c t g a g g c g g g g g a t g c c g

3420 315 320 325 330 VP16 activation domain ALDMADFEFEQMFTDALGID tTA ALDMADFEFEQMFTDALGID g c t c t g g a t a t g g c c g a c t t c g a g t t t g a g c a g a t g t t t a c c g a t g c c c t t g g a a t t g a c c g a g a c c t a t a c c g g c t g a a g c t c a a a c t c g t c t a c a a a t g g c t a c g g g a a c c t t a a c t g

XbaIPspOMIApaI 3480 335 VP16 activation domain EYGG* tTA bGH poly(A) signal G G A G C T G A C A C G G A BGH-rev EYGG* g a g t a c g g t g g g t a g t c t a g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t c t c a t g c c a c c c a t c a g a t c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a

3540 bGH poly(A) signal A G A T BGH-rev t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a

3600 bGH poly(A) signal g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c

3660 bGH poly(A) signal t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g

3720 bGH poly(A) signal a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g

3780 f1 ori t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c

3840 f1 ori G A G G A A A G C G A F1ori-R g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a

3900 f1 ori A A G A A G G G A F1ori-R t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c

3960 f1 ori c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c

4020 f1 ori g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c

4080 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g

4140 f1 ori t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a

4200 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a

4260 SV40 promoter g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a

SexAI*CsiI 4320 SV40 promoter c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g

4380 SV40 promoter SV40 ori a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g

4440 SV40 promoter SV40 ori T A T T T A T G C A G A G G SV40pro-F c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c

StuIAvrII 4500 SV40 promoter SV40 ori C C G A G G SV40pro-F c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g

4560 SV40 promoter t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t

4620 1 5 10 MIEQDGLHAGSPA NeoR/KanR MIEQDGLHAGSPA c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c

4680 15 20 25 30 AWVERLFGYDWAQQTIGCSD NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AWVERLFGYDWAQQTIGCSD c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c

KasINarISfoIPluTI 4740 35 40 45 50 AAVFRLSAQGRPVLFVKTDL NeoR/KanR AAVFRLSAQGRPVLFVKTDL c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a

4800 55 60 65 70 SGALNELQDEAARLSWLATT NeoR/KanR SGALNELQDEAARLSWLATT c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c

4860 75 80 85 90 GVPCAAVLDVVTEAGRDWLL NeoR/KanR GVPCAAVLDVVTEAGRDWLL g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a

4920 95 100 105 110 LGEVPGQDLLSSHLAPAEKV NeoR/KanR LGEVPGQDLLSSHLAPAEKV *RVKSRGLFY t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a

4980 115 120 125 130 SIMADAMRRLHTLDPATCPF NeoR/KanR SIMADAMRRLHTLDPATCPF GDHSICHPPQMRKIRSGAWE c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c

5040 135 140 145 150 DHQAKHRIERARTRMEAGLV NeoR/KanR DHQAKHRIERARTRMEAGLV VVLRFMADLSCTSPHFGTKD a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c

5100 155 160 165 170 DQDDLDEEHQGLAPAELFAR NeoR/KanR DQDDLDEEHQGLAPAELFAR ILIIQVFLMLPERWGFQEGP a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g

5160 175 180 185 190 LKARMPDGEDLVVTHGDACL NeoR/KanR LKARMPDGEDLVVTHGDACL ELRAHGVALIEDHGMAIGAQ t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g

5220 195 200 205 210 PNIMVENGRFSGFIDCGRLG NeoR/KanR PNIMVENGRFSGFIDCGRLG RIDHHFIAAKRSEDVTAPQT c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c

RsrII 5280 215 220 225 230 VADRYQDIALATRDIAEELG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F VADRYQDIALATRDIAEELG HRVAILVYRQSGTINSFLKA t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c

5340 235 240 245 250 GEWADRFLVLYGIAAPDSQR NeoR/KanR GEWADRFLVLYGIAAPDSQR AFPSVAEEHKVTDGSGIRLA g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t

BstBI 5400 255 260 265 IAFYRLLDEFF* NeoR/KanR IAFYRLLDEFF* DGEIAKKVLEESRSEPTRFS t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g

5460 RGLSAWGAM c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t

5520 a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t

5580 SV40 poly(A) signal t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t

5640 SV40 poly(A) signal G EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c

BstZ17I 5700 SV40 poly(A) signal T G G T T T G T C C A A A C T C A T C EBV-rev t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t

5760 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a

5820 -10 -35 lac promoter CAP binding site t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c

5880 CAP binding site c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c

5940 c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g

6000 G A G C G A G T G A C T G A G C G A L4440 t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g

6060 a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t

6120 c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a

6180 ori t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c

6240 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c

6300 ori c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c

6360 ori c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g

6420 ori c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t

6480 ori c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c

6540 ori t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g

6600 ori t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g

6660 ori c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c

6720 ori t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a

6780 g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a

6840 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t

6900 285 *WHKILS AmpR *WHKILS a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t

AhdI 6960 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a

7020 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c

7080 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t

7140 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t

7200 200 195 190 185 ALTLLEGTLLKRLTTAMAVP AmpR ALTLLEGTLLKRLTTAMAVP a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c

7260 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g

7320 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g

PvuI 7380 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t

ScaI 7440 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g

7500 100 95 90 85 LDNQSYHIRRGLQEQGADIR AmpR LDNQSYHIRRGLQEQGADIR c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c

7560 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g

7620 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c

7680 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g

7740 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR PLCFAAFFPILAVRFHQISM a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a

7800 AmpR promoter a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t

7860 AmpR promoter c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t

3ʹ 5ʹ 7874 a g t g c c a c c t g a c g t c a c g g t g g a c t g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.