Addgene GPI_2xmCherry Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: GPI_2xmCherry Sequencing Result


Map View

AseI (5814) pBR322ori-F (5594 .. 5613) EcoO109I (4929) BsaI (4820) TK-pA-R (4685 .. 4704) PfoI (4606) BstBI (4513) Neo-F (4367 .. 4386) RsrII (4347) BsrDI (4064) PflFI - Tth111I (3949) FspI (3933) Neo-R (3757 .. 3776) BspDI * - ClaI * (3671) SfiI (3606) CsiI - SexAI * (3420) pBABE 3' (3334 .. 3354) F1ori-R (3072 .. 3091) DraIII (2947) CMV enhancer NdeI (174) SnaBI (280) CMV-F (459 .. 479) AfeI (536) BlpI (565) SalI (656) AccI (657) BspEI (1371) BamHI (1433) AgeI (1439) BstXI (2335) NotI (2475) HpaI (2594) SV40pA-R (2632 .. 2651) BtsI - BtsαI (2670) EBV-rev (2686 .. 2705) AflII (2713) F1ori-F (2860 .. 2881) GPI_2xmCherry 5867 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

120 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 180 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

240 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 300 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

360 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

420 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

480 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

AfeI 540 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

BlpI 600 Kozak sequence MELFWSI c c g g a c t c a g a t c t c g a g c t c a a g c t t a g c t a g c c a c c a t g g a g c t c t t t t g g a g t a t a g g g c c t g a g t c t a g a g c t c g a g t t c g a a t c g a t c g g t g g t a c c t c g a g a a a a c c t c a t a t c

SalIAccI 660 VFTVLLSFSCRGSDWESLQS *VPFRQLR t c t t t a c t g t c c t c c t g a g t t t c t c c t g c c g g g g g t c a g a c t g g g a a t c t c t g c a g t c g a a g a a a t g a c a g g a g g a c t c a a a g a g g a c g g c c c c c a g t c t g a c c c t t a g a g a c g t c a g c t

720 1 5 10 15 MVSKGEEDNMAIIKEFMRF mCherry TMVSKGEEDNMAIIKEFMRF SHHALALLIVHGDDLLEHAE c t a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a g a t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t

780 20 25 30 35 KVHMEGSVNGHEFEIEGEGE mCherry KVHMEGSVNGHEFEIEGEGE LHVHLAGHVAVLELDLALAL a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c

840 40 45 50 55 GRPYEGTQTAKLKVTKGGPL mCherry GRPYEGTQTAKLKVTKGGPL AAGVLAGLGGLQLHGLTAGQ g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g

900 60 65 70 75 PFAWDILSPQFMYGSKAYVK mCherry PFAWDILSPQFMYGSKAYVK GEGPVDQGRLEHVAGLGVHL c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g c g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t c g

960 80 85 90 95 HPADIPDYLKLSFPEGFKWE mCherry HPADIPDYLKLSFPEGFKWE VGGVDGVVQLQGEGLAELPL a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g

1020 100 105 110 115 RVMNFEDGGVVTVTQDSSLQ mCherry RVMNFEDGGVVTVTQDSSLQ AHHVELVAAHHGHGLVGGQL g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c

1080 120 125 130 135 DGEFIYKVKLRGTNFPSDGP mCherry DGEFIYKVKLRGTNFPSDGP VALEDVLHLQAAGVEGGVAG a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c g t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g g c

1140 140 145 150 155 VMQKKTMGWEASSERMYPED mCherry VMQKKTMGWEASSERMYPED YHLLLGHAPLGGGLPHVGLV t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a c g a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t g c

1200 160 165 170 175 GALKGEIKQRLKLKDGGHYD mCherry GALKGEIKQRLKLKDGGHYD AGQLALDLLPQLQLVAAVVV g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a c g c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t g c

1260 180 185 190 195 AEVKTTYKAKKPVQLPGAYN mCherry AEVKTTYKAKKPVQLPGAYN SLDLGGVLGLLGHLQGAGVV c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a c g g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t g c

1320 200 205 210 215 VNIKLDITSHNEDYTIVEQY mCherry VNIKLDITSHNEDYTIVEQY DVDLQVDGGVVLVVGDHFLV t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c

BspEI 1380 220 225 230 235 ERAEGRHSTGGMDELYK SGG mCherry ERAEGRHSTGGMDELYKSGG FAGLAAVGGAAHVLQVLGST a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t c c g g a g g t c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a g g c c t c c a g

AgeIBamHI 1440 (in frame with mCherry) HGTGSTGSGSSRSRAQASDP HGTGSTGSGSSRSRAQASDP MTSTRSTRTRRSRSSLSRIW a t g g t a c t g g t t c t a c t g g t t c t g g t t c t t c t a g a t c t c g a g c t c a a g c t t c g g a t c c a c t a c c a t g a c c a a g a t g a c c a a g a c c a a g a a g a t c t a g a g c t c g a g t t c g a a g c c t a g g t g

1500 PV 1 5 10 15 MVSKGEEDNMAIIKEFMR mCherry PVMVSKGEEDNMAIIKEFMR RHHHALALLIVHGDDLLEHA c g g t g a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t g c c a c t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a

1560 20 25 30 35 FKVHMEGSVNGHEFEIEGEG mCherry FKVHMEGSVNGHEFEIEGEG ELHVHLAGHVAVLELDLALA t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c

1620 40 45 50 55 EGRPYEGTQTAKLKVTKGGP mCherry EGRPYEGTQTAKLKVTKGGP LAAGVLAGLGGLQLHGLTAG a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g

1680 60 65 70 75 LPFAWDILSPQFMYGSKAYV mCherry LPFAWDILSPQFMYGSKAYV QGEGPVDQGRLEHVAGLGVH t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t

1740 80 85 90 95 KHPADIPDYLKLSFPEGFKW mCherry KHPADIPDYLKLSFPEGFKW LVGGVDGVVQLQGEGLAELP a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c

1800 100 105 110 115 ERVMNFEDGGVVTVTQDSSL mCherry ERVMNFEDGGVVTVTQDSSL LAHHVELVAAHHGHGLVGGQ a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g

1860 120 125 130 135 QDGEFIYKVKLRGTNFPSDG mCherry QDGEFIYKVKLRGTNFPSDG LVALEDVLHLQAAGVEGGVA a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g

1920 140 145 150 155 PVMQKKTMGWEASSERMYPE mCherry PVMQKKTMGWEASSERMYPE GYHLLLGHAPLGGGLPHVGL c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c

1980 160 165 170 175 DGALKGEIKQRLKLKDGGHY mCherry DGALKGEIKQRLKLKDGGHY VAGQLALDLLPQLQFVAAVV a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a a g a c g g c g g c c a c t a c g t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t t c t g c c g c c g g t g a t g c

2040 180 185 190 195 DAEVKTTYKAKKPVQLPGAY mCherry DAEVKTTYKAKKPVQLPGAY VSLDLGGVLGLLGHLQGAGV a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t

2100 200 205 210 215 NVNIKLDITSHNEDYTIVEQ mCherry NVNIKLDITSHNEDYTIVEQ VDVDLQVDGGVVLVVGDHFL a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a

2160 220 225 230 235 YERAEGRHSTGGMDELYK YD mCherry YERAEGRHSTGGMDELYKYD VFAGLAAVGGAAHVLQVLII a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t a t g a t c t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a t a c t a g

2220 (in frame with mCherry) PRPSSGHSRHRYALIPIPLA PRPSSGHSRHRYALIPIPLA RAWAATM c g c g c c c a a g c a g c g g t c a t t c t a g a c a c a g a t a t g c a c t t a t a c c c a t a c c a t t a g c a g g c g c g g g t t c g t c g c c a g t a a g a t c t g t g t c t a t a c g t g a a t a t g g g t a t g g t a a t c g t c

2280 (in frame with mCherry) VITTCIVLYMNVL* VITTCIVLYMNVL* t a a t t a c a a c a t g t a t t g t g c t g t a t a t g a a t g t t c t t t a a t t g a g a a g a c a a t t t c t t c a t t a a t g t t g t a c a t a a c a c g a c a t a t a c t t a c a a g a a a t t a a c t c t t c t g t t a a a g a a g

BstXI 2340 a t t t t t a g g t a t t c t g a a a t g t g a c a g a a a a c c a g a c a g a a c c a a c t c c a a t t g a t t g g t t a a a a a t c c a t a a g a c t t t a c a c t g t c t t t t g g t c t g t c t t g g t t g a g g t t a a c t a a c c a

2400 a a c a g a a g a t g a a g a c a a c a g c a t a a c t a a a t t a t t t t a a a a a c t a a a a a g c c a t c t g a t t t g t c t t c t a c t t c t g t t g t c g t a t t g a t t t a a t a a a a t t t t t g a t t t t t c g g t a g a c t a

2460 t t c t c a t t t g a g c t t t a g a g c a c a a t g g a t c t c g a g g g a t c t t c c a t a c c t a c c a g t t c t a a g a g t a a a c t c g a a a t c t c g t g t t a c c t a g a g c t c c c t a g a a g g t a t g g a t g g t c a a g a

NotI 2520 g c g c c t g c a g g t c g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g c g c g g a c g t c c a g c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c

2580 t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c

HpaI 2640 SV40 poly(A) signal C G T T A T C G T SV40pA-R c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t

BtsαIBtsI 2700 SV40 poly(A) signal G T G G T T T G T C C A A A C EBV-rev A G T G T T T A A A G SV40pA-R t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g

AflII 2760 SV40 poly(A) signal f1 ori T C A T C EBV-rev t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t

2820 f1 ori a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a

2880 f1 ori G G T C A A A C C T T G T T C T C A G G T F1ori-F a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t

2940 f1 ori G F1ori-F c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g

DraIII 3000 f1 ori c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t

3060 f1 ori a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c

3120 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c

3180 f1 ori g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t

3240 AmpR promoter g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a

3300 AmpR promoter t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t

3360 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t

3420 SV40 promoter SV40 enhancer a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t

SexAI*CsiI 3480 SV40 promoter SV40 enhancer c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t

3540 SV40 promoter SV40 enhancer SV40 ori a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

3600 SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

SfiI 3660 SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

BspDI*ClaI* 3720 1 5 MIEQDG SV40 promoter NeoR/KanR 1 5 MIEQDG NeoR/KanR MIEQDG t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t

3780 10 15 20 25 LHAGSPAAWVERLFGYDWAQ NeoR/KanR 10 15 20 25 LHAGSPAAWVERLFGYDWAQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LHAGSPAAWVERLFGYDWAQ t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t

3840 30 35 40 45 QTIGCSDAAVFRLSAQGRPV NeoR/KanR 30 35 40 45 QTIGCSDAAVFRLSAQGRPV NeoR/KanR QTIGCSDAAVFRLSAQGRPV c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a

3900 50 55 60 65 LFVKTDLSGALNELQDEAAR NeoR/KanR 50 55 60 65 LFVKTDLSGALNELQDEAAR NeoR/KanR LFVKTDLSGALNELQDEAAR c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c

Tth111IPflFIFspI 3960 70 75 80 85 LSWLATTGVPCAAVLDVVTE NeoR/KanR 70 75 80 85 LSWLATTGVPCAAVLDVVTE NeoR/KanR LSWLATTGVPCAAVLDVVTE c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t

4020 90 95 100 105 AGRDWLLLGEVPGQDLLSSH NeoR/KanR 90 95 100 105 AGRDWLLLGEVPGQDLLSSH NeoR/KanR AGRDWLLLGEVPGQDLLSSH *RV g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g

BsrDI 4080 110 115 120 125 LAPAEKVSIMADAMRRLHTL NeoR/KanR 110 115 120 125 LAPAEKVSIMADAMRRLHTL NeoR/KanR LAPAEKVSIMADAMRRLHTL KSRGLFYGDHSICHPPQMRK c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a

4140 130 135 140 145 DPATCPFDHQAKHRIERART NeoR/KanR 130 135 140 145 DPATCPFDHQAKHRIERART NeoR/KanR DPATCPFDHQAKHRIERART IRSGAWEVVLRFMADLSCTS g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a

4200 150 155 160 165 RMEAGLVDQDDLDEEHQGLA NeoR/KanR 150 155 160 165 RMEAGLVDQDDLDEEHQGLA NeoR/KanR RMEAGLVDQDDLDEEHQGLA PHFGTKDILIIQVFLMLPER c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c

4260 170 175 180 185 PAELFARLKASMPDGEDLVV NeoR/KanR 170 175 180 185 PAELFARLKASMPDGEDLVV NeoR/KanR PAELFARLKASMPDGEDLVV WGFQEGPELRAHGVALIEDH c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c

4320 190 195 200 205 THGDACLPNIMVENGRFSGF NeoR/KanR 190 195 200 205 THGDACLPNIMVENGRFSGF NeoR/KanR THGDACLPNIMVENGRFSGF GMAIGAQRIDHHFIAAKRSE a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g

RsrII 4380 210 215 220 225 IDCGRLGVADRYQDIALATR NeoR/KanR 210 215 220 225 IDCGRLGVADRYQDIALATR NeoR/KanR C G T T G G C T A C C C G T Neo-F IDCGRLGVADRYQDIALATR DVTAPQTHRVAILVYRQSGT a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a

4440 230 235 240 245 DIAEELGGEWADRFLVLYGI NeoR/KanR 230 235 240 245 DIAEELGGEWADRFLVLYGI NeoR/KanR G A T A T T Neo-F DIAEELGGEWADRFLVLYGI INSFLKAAFPSVAEEHKVTD g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g

4500 250 255 260 265 AAPDSQRIAFYRLLDEFF* NeoR/KanR 250 255 260 265 AAPDSQRIAFYRLLDEFF* NeoR/KanR AAPDSQRIAFYRLLDEFF* GSGIRLADGEIAKKVLEESR g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c

BstBI 4560 MTDQATPNLPSRDF SEPTRFSRGLSAWGAM g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c

PfoI 4620 DSTAAFYERLGFGIVFRDAG a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a

4680 WMILQRGDLMLEFFAHPRGR g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g

4740 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R LTETRKETIPEGTRAMTAIK t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t

4800 HSV TK poly(A) signal RQNKTHGVGSFVHKRGVRSQ g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c

BsaI 4860 GWHSVDTPPRPHWGQYARVS g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a

4920 SFSPPHPPSSGEGPGLAANV c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c

EcoO109I 4980 GAAGPAIASGYSYIL* g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g

5040 t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t

5100 t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a

5160 ori c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g

5220 ori t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c

5280 ori g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g

5340 ori a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c

5400 ori c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c

5460 ori a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t

5520 ori c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c

5580 ori a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t

5640 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a

5700 ori g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t

5760 g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g

AseI 5820 c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t t a a t a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a a t t a t c

3ʹ 5ʹ 5867 t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.