Addgene pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* Sequencing Result


Map View

AatII (13,324) ZraI (13,322) Amp-R (12,952 .. 12,971) pBR322ori-F (11,650 .. 11,669) PciI (11,509) L4440 (11,399 .. 11,416) NdeI (11,332) pRS-marker (11,299 .. 11,318) pGEX 3' (11,136 .. 11,158) SphI (10,267) pBRrevBam (10,139 .. 10,158) DraIII (10,076) pENTR-R (9966 .. 9985) NruI (9885) Kan-R (9882 .. 9901) SmaI (9668) TspMI - XmaI (9666) AsiSI (9544) StuI * (8918) BbvCI (8875) FspAI (8593) BmtI (8564) NheI (8560) KpnI (7855) Acc65I (7851) EcoRI (7776) pBRforEco (13,362 .. 13,380) Eco53kI (299) SacI (301) PmeI (506) BlpI (681) SpeI (1032) 6xHis BsrGI (4037) BmgBI (4269) BamHI (4392) 6xHis pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* 13,406 bp

Sequence View

5ʹ 3ʹ 60 a t g t t t g a c a g c t t a t c a t c g a t a a g c t g a c t c a t g t t g g t a t t g t g a a a t a g a c g c a g a t a c a a a c t g t c g a a t a g t a g c t a t t c g a c t g a g t a c a a c c a t a a c a c t t t a t c t g c g t c t

120 AOX1 promoter t c g g g a a c a c t g a a a a a t a a c a g t t a t t a t t c g a g a t c t a a c a t c c a a a g a c g a a a g g t t a g c c c t t g t g a c t t t t t a t t g t c a a t a a t a a g c t c t a g a t t g t a g g t t t c t g c t t t c c a a

180 AOX1 promoter g a a t g a a a c c t t t t t g c c a t c c g a c a t c c a c a g g t c c a t t c t c a c a c a t a a g t g c c a a a c c t t a c t t t g g a a a a a c g g t a g g c t g t a g g t g t c c a g g t a a g a g t g t g t a t t c a c g g t t t g

240 AOX1 promoter g c a a c a g g a g g g g a t a c a c t a g c a g c a g a c c g t t g c a a a c g c a g g a c c t c c a c t c c t c t t c g t t g t c c t c c c c t a t g t g a t c g t c g t c t g g c a a c g t t t g c g t c c t g g a g g t g a g g a g a a

Eco53kI 300 AOX1 promoter c t c c t c a a c a c c c a c t t t t g c c a t c g a a a a a c c a g c c c a g t t a t t g g g c t t g a t t g g a g c g a g g a g t t g t g g g t g a a a a c g g t a g c t t t t t g g t c g g g t c a a t a a c c c g a a c t a a c c t c g

SacI 360 AOX1 promoter t c g c t c a t t c c a a t t c c t t c t a t t a g g c t a c t a a c a c c a t g a c t t t a t t a g c c t g t c t a t a g c g a g t a a g g t t a a g g a a g a t a a t c c g a t g a t t g t g g t a c t g a a a t a a t c g g a c a g a t a

420 AOX1 promoter c c t g g c c c c c c t g g c g a g g t t c a t g t t t g t t t a t t t c c g a a t g c a a c a a g c t c c g c a t t a g g a c c g g g g g g a c c g c t c c a a g t a c a a a c a a a t a a a g g c t t a c g t t g t t c g a g g c g t a a t

480 AOX1 promoter c a c c c g a a c a t c a c t c c a g a t g a g g g c t t t c t g a g t g t g g g g t c a a a t a g t t t c a t g t t c g t g g g c t t g t a g t g a g g t c t a c t c c c g a a a g a c t c a c a c c c c a g t t t a t c a a a g t a c a a g

PmeI 540 AOX1 promoter c c c a a a t g g c c c a a a a c t g a c a g t t t a a a c g c t g t c t t g g a a c c t a a t a t g a c a a a a g c g g g g t t t a c c g g g t t t t g a c t g t c a a a t t t g c g a c a g a a c c t t g g a t t a t a c t g t t t t c g c

600 AOX1 promoter t g a t c t c a t c c a a g a t g a a c t a a g t t t g g t t c g t t g a a a t g c t a a c g g c c a g t t g g t c a a a c t a g a g t a g g t t c t a c t t g a t t c a a a c c a a g c a a c t t t a c g a t t g c c g g t c a a c c a g t t

660 AOX1 promoter a a a g a a a c t t c c a a a a g t c g g c a t a c c g t t t g t c t t g t t t g g t a t t g a t t g a c g a a t g c t t t t c t t t g a a g g t t t t c a g c c g t a t g g c a a a c a g a a c a a a c c a t a a c t a a c t g c t t a c g a

BlpI 720 AOX1 promoter c a a a a a t a a t c t c a t t a a t g c t t a g c g c a g t c t c t c t a t c g c t t c t g a a c c c c g g t g c a c g t t t t t a t t a g a g t a a t t a c g a a t c g c g t c a g a g a g a t a g c g a a g a c t t g g g g c c a c g t g

780 AOX1 promoter c t g t g c c g a a a c g c a a a t g g g g a a a c a c c c g c t t t t t g g a t g a t t a t g c a t t g t c t c c a c g a c a c g g c t t t g c g t t t a c c c c t t t g t g g g c g a a a a a c c t a c t a a t a c g t a a c a g a g g t g

840 AOX1 promoter a t t g t a t g c t t c c a a g a t t c t g g t g g g a a t a c t g c t g a t a g c c t a a c g t t c a t g a t c a a a t a a c a t a c g a a g g t t c t a a g a c c a c c c t t a t g a c g a c t a t c g g a t t g c a a g t a c t a g t t t

900 AOX1 promoter a t t t a a c t g t t c t a a c c c c t a c t t g a c a g c a a t a t a t a a a c a g a a g g a a g c t g c c c t g t c t a a a t t g a c a a g a t t g g g g a t g a a c t g t c g t t a t a t a t t t g t c t t c c t t c g a c g g g a c a g

960 AOX1 promoter t t a a a c c t t t t t t t t t a t c a t c a t t a t t a g c t t a c t t t c a t a a t t g c g a c t g g t t c c a a t a a t t t g g a a a a a a a a a t a g t a g t a a t a a t c g a a t g a a a g t a t t a a c g c t g a c c a a g g t t a

1020 AOX1 promoter t g a c a a g c t t t t g a t t t t a a c g a c t t t t a a c g a c a a c t t g a g a a g a t c a a a a a a c a a c t a a c t g t t c g a a a a c t a a a a t t g c t g a a a a t t g c t g t t g a a c t c t t c t a g t t t t t t g t t g a t

SpeI 1080 AOX1 promoter GAP promoter a t t a t t c g a a g a c t a g t c t t t t t g t a g a a a t g t c t t g g t g t c c t c g t c c a a t c a g g t a g c t a a t a a g c t t c t g a t c a g a a a a a c a t c t t t a c a g a a c c a c a g g a g c a g g t t a g t c c a t c g

1140 GAP promoter c a t c t c t g a a a t a t c t g g c t c c g t t g c a a c t c c g a a c g a c c t g c t g g c a a c g t a a a a t t c g t a g a g a c t t t a t a g a c c g a g g c a a c g t t g a g g c t t g c t g g a c g a c c g t t g c a t t t t a a g

1200 GAP promoter t c c g g g g t a a a a c t t a a a t g t g g a g t a a t g g a a c c a g a a a c g t c t c t t c c c t t c t c t c t c a g g c c c c a t t t t g a a t t t a c a c c t c a t t a c c t t g g t c t t t g c a g a g a a g g g a a g a g a g a g

1260 GAP promoter c t t c c a c c g c c c g t t a c c g t c g c t a g g a a a t t t t a c t c t g c t g g a g a g c t t c t t c t a c g g g a a g g t g g c g g g c a a t g g c a g c g a t c c t t t a a a a t g a g a c g a c c t c t c g a a g a a g a t g c c

1320 GAP promoter c c c c c t t g c a g c a a t g c t c t t c c c a g c a t t a c g t t g c g g g t a a a a c g g a g g t c g t g t a c c g g g g g a a c g t c g t t a c g a g a a g g g t c g t a a t g c a a c g c c c a t t t t g c c t c c a g c a c a t g g

1380 GAP promoter c g a c c t a g c a g c c c a g g g a t g g a a a a g t c c c g g c c g t c g c t g g c a a t a a t a g c g g g c g g a g c t g g a t c g t c g g g t c c c t a c c t t t t c a g g g c c g g c a g c g a c c g t t a t t a t c g c c c g c c t

1440 GAP promoter c g c a t g t c a t g a g a t t a t t g g a a a c c a c c a g a a t c g a a t a t a a a a g g c g a a c a c c t t t c c g c g t a c a g t a c t c t a a t a a c c t t t g g t g g t c t t a g c t t a t a t t t t c c g c t t g t g g a a a g g

1500 GAP promoter c a a t t t t g g t t t c t c c t g a c c c a a a g a c t t t a a a t t t a a t t t a t t t g t c c c t a t t t c a a t g t t a a a a c c a a a g a g g a c t g g g t t t c t g a a a t t t a a a t t a a a t a a a c a g g g a t a a a g t t a

1560 GAP promoter MSIPETQKAIIFYES c a a t t g a a c a a c t a t a t g t c t a t t c c a g a a a c t c a a a a a g c c a t t a t c t t c t a c g a a t c c g t t a a c t t g t t g a t a t a c a g a t a a g g t c t t t g a g t t t t t c g g t a a t a g a a g a t g c t t a g g

1620 NGKLEHKDIPVPKPKPNELL a a c g g c a a g t t g g a g c a t a a g g a t a t c c c a g t t c c a a a g c c a a a g c c c a a c g a a t t g t t a t t g c c g t t c a a c c t c g t a t t c c t a t a g g g t c a a g g t t t c g g t t t c g g g t t g c t t a a c a a t

1680 INVKYSGVCHTDLHAWHGDW a t c a a c g t c a a g t a c t c t g g t g t c t g c c a c a c c g a t t t g c a c g c t t g g c a t g g t g a c t g g t a g t t g c a g t t c a t g a g a c c a c a g a c g g t g t g g c t a a a c g t g c g a a c c g t a c c a c t g a c c

1740 PLPTKLPLVGGHEGAGVVVG c c a t t g c c a a c t a a g t t a c c a t t a g t t g g t g g t c a c g a a g g t g c c g g t g t c g t t g t c g g c g g t a a c g g t t g a t t c a a t g g t a a t c a a c c a c c a g t g c t t c c a c g g c c a c a g c a a c a g c c g

1800 MGENVKGWKIGDYAGIKWLN a t g g g t g a a a a c g t t a a g g g c t g g a a g a t c g g t g a c t a c g c c g g t a t c a a a t g g t t g a a c t a c c c a c t t t t g c a a t t c c c g a c c t t c t a g c c a c t g a t g c g g c c a t a g t t t a c c a a c t t g

1860 GSCMACEYCELGNESNCPHA g g t t c t t g t a t g g c c t g t g a a t a c t g t g a a t t g g g t a a c g a a t c c a a c t g t c c t c a c g c t c c a a g a a c a t a c c g g a c a c t t a t g a c a c t t a a c c c a t t g c t t a g g t t g a c a g g a g t g c g a

1920 DLSGYTHDGSFQEYATADAV g a c t t g t c t g g t t a c a c c c a c g a c g g t t c t t t c c a a g a a t a c g c t a c c g c t g a c g c t g t t c t g a a c a g a c c a a t g t g g g t g c t g c c a a g a a a g g t t c t t a t g c g a t g g c g a c t g c g a c a a

1980 QAAHIPQGTDLAEVAPILCA c a a g c c g c t c a c a t t c c t c a a g g t a c t g a c t t g g c t g a a g t c g c g c c a a t c t t g t g t g c t g t t c g g c g a g t g t a a g g a g t t c c a t g a c t g a a c c g a c t t c a g c g c g g t t a g a a c a c a c g a

2040 GITVYKALKSANLRAGHWAA g g t a t c a c c g t a t a c a a g g c t t t g a a g t c t g c c a a c t t g a g a g c a g g c c a c t g g g c g g c c c c a t a g t g g c a t a t g t t c c g a a a c t t c a g a c g g t t g a a c t c t c g t c c g g t g a c c c g c c g g

2100 ISGAAGGLGSLAVQYAKAMG a t t t c t g g t g c t g c t g g t g g t c t a g g t t c t t t g g c t g t t c a a t a t g c t a a g g c g a t g g g t t a a a g a c c a c g a c g a c c a c c a g a t c c a a g a a a c c g a c a a g t t a t a c g a t t c c g c t a c c c a

2160 YRVLGIDGGPGKEELFTSLG t a c a g a g t c t t a g g t a t t g a t g g t g g t c c a g g a a a g g a a g a a t t g t t t a c c t c g c t c g g t a t g t c t c a g a a t c c a t a a c t a c c a c c a g g t c c t t t c c t t c t t a a c a a a t g g a g c g a g c c a

2220 GEVFIDFTKEKDIVSAVVKA g g t g a a g t a t t c a t c g a c t t c a c c a a a g a g a a g g a c a t t g t t a g c g c a g t c g t t a a g g c t c c a c t t c a t a a g t a g c t g a a g t g g t t t c t c t t c c t g t a a c a a t c g c g t c a g c a a t t c c g a

2280 TNGGAHGIINVSVSEAAIEA a c c a a c g g c g g t g c c c a c g g t a t c a t c a a t g t t t c c g t t t c c g a a g c c g c t a t c g a a g c t t g g t t g c c g c c a c g g g t g c c a t a g t a g t t a c a a a g g c a a a g g c t t c g g c g a t a g c t t c g a

2340 STRYCRANGTVVLVGLPAGA t c t a c c a g a t a c t g t a g g g c g a a c g g t a c t g t t g t c t t g g t t g g t t t g c c a g c c g g t g c a a g a t g g t c t a t g a c a t c c c g c t t g c c a t g a c a a c a g a a c c a a c c a a a c g g t c g g c c a c g t

2400 KCSSDVFNHVVKSISIVGSY a a g t g c t c c t c t g a t g t c t t c a a c c a c g t t g t c a a g t c t a t c t c c a t t g t c g g c t c t t a c t t c a c g a g g a g a c t a c a g a a g t t g g t g c a a c a g t t c a g a t a g a g g t a a c a g c c g a g a a t g

2460 VGNRADTREALDFFARGLVK g t g g g g a a c a g a g c t g a t a c c a g a g a a g c c t t a g a t t t c t t t g c c a g a g g t c t a g t c a a g c a c c c c t t g t c t c g a c t a t g g t c t c t t c g g a a t c t a a a g a a a c g g t c t c c a g a t c a g t t c

2520 SPIKVVGLSSLPEIYEKMEK t c t c c a a t a a a g g t a g t t g g c t t a t c c a g t t t a c c a g a a a t t t a c g a a a a g a t g g a g a a g a g a g g t t a t t t c c a t c a a c c g a a t a g g t c a a a t g g t c t t t a a a t g c t t t t c t a c c t c t t c

2580 GQIAGRYVVDTSK* g g c c a a a t t g c t g g t a g a t a c g t t g t t g a c a c t t c t a a a t a a t c g a g c c g c g g c g g c c g c c c g g t t t a a c g a c c a t c t a t g c a a c a a c t g t g a a g a t t t a t t a g c t c g g c g c c g c c g g c g

2640 1 5 10 EQKLISEEDL (in frame with Myc) NSAVD 1 HH Myc 6xHis c a g c t t t c t a g a a c a a a a a c t c a t c t c a g a a g a g g a t c t g a a t a g c g c c g t c g a c c a t c a g t c g a a a g a t c t t g t t t t t g a g t a g a g t c t t c t c c t a g a c t t a t c g c g g c a g c t g g t a g t

2700 5 HHHH * 6xHis t c a t c a t c a t c a t t g a g t t t g t a g c c t t a g a c a t g a c t g t t c c t c a g t t c a a g t t g g g c a a g t a g t a g t a g t a a c t c a a a c a t c g g a a t c t g t a c t g a c a a g g a g t c a a g t t c a a c c c g t

2760 AOX1 terminator c t t a c g a g a a g a c c g g t c t t g c t a g a t t c t a a t c a a g a g g a t g t c a g a a t g c c a t t t g c c g a a t g c t c t t c t g g c c a g a a c g a t c t a a g a t t a g t t c t c c t a c a g t c t t a c g g t a a a c g g

2820 AOX1 terminator t g a g a g a t g c a g g c t t c a t t t t t g a t a c t t t t t t a t t t g t a a c c t a t a t a g t a t a g g a t t a c t c t c t a c g t c c g a a g t a a a a a c t a t g a a a a a a t a a a c a t t g g a t a t a t c a t a t c c t a a

2880 AOX1 terminator t t t t t t g t c a t t t t g t t t c t t c t c g t a c g a g c t t g c t c c t g a t c a g c c t a t c t c g c a g c t a a a a a a c a g t a a a a c a a a g a a g a g c a t g c t c g a a c g a g g a c t a g t c g g a t a g a g c g t c g a

2940 AOX1 terminator g a t g a a t a t c t t g t g g t a g g g g t t t g g g a a a a t c a t t c g a g t t t g a t g t t t t t c t t g g t a c t a c t t a t a g a a c a c c a t c c c c a a a c c c t t t t a g t a a g c t c a a a c t a c a a a a a g a a c c a t

3000 AOX1 terminator GAP promoter t t t c c c a c t c c t c t t c a g a g t a c a g a a g a t t a a g t g a g a t t t t t g t a g a a a t g t c t t g g t a a a g g g t g a g g a g a a g t c t c a t g t c t t c t a a t t c a c t c t a a a a a c a t c t t t a c a g a a c c a

3060 GAP promoter g t c c t c g t c c a a t c a g g t a g c c a t c t c t g a a a t a t c t g g c t c c g t t g c a a c t c c g a a c g a c a g g a g c a g g t t a g t c c a t c g g t a g a g a c t t t a t a g a c c g a g g c a a c g t t g a g g c t t g c t

3120 GAP promoter c c t g c t g g c a a c g t a a a a t t c t c c g g g g t a a a a c t t a a a t g t g g a g t a a t g g a a c c a g a a g g a c g a c c g t t g c a t t t t a a g a g g c c c c a t t t t g a a t t t a c a c c t c a t t a c c t t g g t c t t

3180 GAP promoter a c g t c t c t t c c c t t c t c t c t c c t t c c a c c g c c c g t t a c c g t c c c t a g g a a a t t t t a c t c t t g c a g a g a a g g g a a g a g a g a g g a a g g t g g c g g g c a a t g g c a g g g a t c c t t t a a a a t g a g a

3240 GAP promoter g c t g g a g a g c t t c t t c t a c g g c c c c c t t g c a g c a a t g c t c t t c c c a g c a t t a c g t t g c g g c g a c c t c t c g a a g a a g a t g c c g g g g g a a c g t c g t t a c g a g a a g g g t c g t a a t g c a a c g c c

3300 GAP promoter g t a a a a c g g a g g t c g t g t a c c c g a c c t a g c a g c c c a g g g a t g g a a a a g t c c c g g c c g t c g c a t t t t g c c t c c a g c a c a t g g g c t g g a t c g t c g g g t c c c t a c c t t t t c a g g g c c g g c a g c

3360 GAP promoter c t g g c a a t a a t a g c g g g c g g a c g c a t g t c a t g a g a t t a t t g g a a a c c a c c a g a a t c g a a t g a c c g t t a t t a t c g c c c g c c t g c g t a c a g t a c t c t a a t a a c c t t t g g t g g t c t t a g c t t a

3420 GAP promoter a t a a a a g g c g a a c a c c t t t c c c a a t t t t g g t t t c t c c t g a c c c a a a g a c t t t a a a t t t a a t a t t t t c c g c t t g t g g a a a g g g t t a a a a c c a a a g a g g a c t g g g t t t c t g a a a t t t a a a t t

3480 GAP promoter MSPSAVQS t t t a t t t g t c c c t a t t t c a a t c a a t t g a a c a a c t a t a t g t c g c c c t c t g c c g t a c a a t c a a a a t a a a c a g g g a t a a a g t t a g t t a a c t t g t t g a t a t a c a g c g g g a g a c g g c a t g t t a g t

3540 SKLEEQSSEIDKLKAKMSQS t c a a a a c t a g a a g a a c a g t c a a g t g a a a t t g a c a a g t t g a a a g c a a a a a t g t c c c a g t c t a g t t t t g a t c t t c t t g t c a g t t c a c t t t a a c t g t t c a a c t t t c g t t t t t a c a g g g t c a g a

3600 ASTAQQKKEHEYEHLTSVKI g c c t c c a c t g c g c a g c a g a a g a a g g a a c a t g a g t a t g a a c a t t t g a c c t c g g t c a a g a t c c g g a g g t g a c g c g t c g t c t t c t t c c t t g t a c t c a t a c t t g t a a a c t g g a g c c a g t t c t a g

3660 VPQRPISDRLQPAIATHYSP g t g c c a c a a c g g c c c a t c t c a g a t a g a c t g c a g c c c g c a a t t g c t a c c c a c t a t t c t c c a c a c g g t g t t g c c g g g t a g a g t c t a t c t g a c g t c g g g c g t t a a c g a t g g g t g a t a a g a g g t

3720 HLDGLQDYQRLHKESIEDPA c a c t t g g a c g g g t t g c a g g a c t a t c a g c g c t t g c a c a a g g a g t c t a t t g a a g a c c c t g c t g t g a a c c t g c c c a a c g t c c t g a t a g t c g c g a a c g t g t t c c t c a g a t a a c t t c t g g g a c g a

3780 KFFGSKATQFLNWSKPFDKV a a g t t c t t c g g t t c t a a a g c t a c c c a a t t t t t a a a c t g g t c t a a g c c a t t c g a t a a g g t g t t c a a g a a g c c a a g a t t t c g a t g g g t t a a a a a t t t g a c c a g a t t c g g t a a g c t a t t c c a c

3840 FIPDSKTGRPSFQNNAWFLN t t c a t c c c a g a c t c t a a a a c g g g t a g g c c c t c c t t c c a g a a c a a t g c a t g g t t c c t c a a c a a g t a g g g t c t g a g a t t t t g c c c a t c c g g g a g g a a g g t c t t g t t a c g t a c c a a g g a g t t g

3900 GQLNACYNCVDRHALKTPNK g g c c a a t t a a a c g c c t g t t a c a a c t g t g t t g a c a g a c a t g c c t t g a a g a c c c c t a a c a a g c c g g t t a a t t t g c g g a c a a t g t t g a c a c a a c t g t c t g t a c g g a a c t t c t g g g g a t t g t t c

3960 KAIIFEGDEPGQGYSITYKE a a a g c c a t t a t t t t c g a a g g t g a c g a g c c t g g c c a a g g c t a t t c c a t t a c c t a c a a g g a a t t t c g g t a a t a a a a g c t t c c a c t g c t c g g a c c g g t t c c g a t a a g g t a a t g g a t g t t c c t t

4020 LLEEVCQVAQVLTYSMGVRK c t a c t t g a a g a a g t t t g t c a a g t g g c a c a a g t g c t g a c t t a c t c t a t g g g c g t t c g c a a g g a t g a a c t t c t t c a a a c a g t t c a c c g t g t t c a c g a c t g a a t g a g a t a c c c g c a a g c g t t c

BsrGI 4080 GDTVAVYMPMVPEAIITLLA g g c g a t a c t g t t g c c g t g t a c a t g c c t a t g g t c c c a g a a g c a a t c a t a a c c t t g t t g g c c c c g c t a t g a c a a c g g c a c a t g t a c g g a t a c c a g g g t c t t c g t t a g t a t t g g a a c a a c c g g

4140 ISRIGAIHSVVFAGFSSNSL a t t t c c c g t a t c g g c g c c a t t c a c t c c g t a g t c t t t g c c g g g t t t t c t t c c a a c t c c t t g t a a a g g g c a t a g c c g c g g t a a g t g a g g c a t c a g a a a c g g c c c a a a a g a a g g t t g a g g a a c

4200 RDRINDGDSKVVITTDESNR a g a g a t c g t a t c a a c g a t g g g g a c t c t a a a g t t g t c a t c a c t a c a g a t g a a t c c a a c a g a t c t c t a g c a t a g t t g c t a c c c c t g a g a t t t c a a c a g t a g t g a t g t c t a c t t a g g t t g t c t

4260 GGKVIETKRIVDDALRETPG g g t g g t a a a g t c a t t g a g a c t a a a a g a a t t g t t g a t g a c g c g c t a a g a g a g a c c c c a g g c c c a c c a t t t c a g t a a c t c t g a t t t t c t t a a c a a c t a c t g c g c g a t t c t c t c t g g g g t c c g

BmgBI 4320 VRHVLVYRKTNNPSVAFHAP g t g a g a c a c g t c t t g g t t t a t a g a a a g a c c a a c a a t c c a t c t g t t g c t t t c c a t g c c c c c c a c t c t g t g c a g a a c c a a a t a t c t t t c t g g t t g t t a g g t a g a c a a c g a a a g g t a c g g g g g

4380 RDLDWATEKKKYKTYYPCTP a g a g a t t t a g a t t g g g c a a c a g a a a a g a a g a a a t a c a a g a c c t a c t a t c c a t g c a c a c c c t c t c t a a a t c t a a c c c g t t g t c t t t t c t t c t t t a t g t t c t g g a t g a t a g g t a c g t g t g g g

BamHI 4440 VDSEDPLFLLYTSGSTGAPK g t t g a t t c t g a g g a t c c a t t a t t c t t g t t g t a t a c g t c t g g t t c t a c t g g t g c c c c c a a g c a a c t a a g a c t c c t a g g t a a t a a g a a c a a c a t a t g c a g a c c a a g a t g a c c a c g g g g g t t c

4500 GVQHSTAGYLLGALLTMRYT g g t g t t c a a c a t t c t a c c g c a g g t t a c t t g c t g g g a g c t t t g t t g a c c a t g c g c t a c a c t c c a c a a g t t g t a a g a t g g c g t c c a a t g a a c g a c c c t c g a a a c a a c t g g t a c g c g a t g t g a

4560 FDTHQEDVFFTAGDIGWITG t t t g a c a c t c a c c a a g a a g a c g t t t t c t t c a c a g c t g g a g a c a t t g g c t g g a t t a c a g g c a a a c t g t g a g t g g t t c t t c t g c a a a a g a a g t g t c g a c c t c t g t a a c c g a c c t a a t g t c c g

4620 HTYVVYGPLLYGCATLVFEG c a c a c t t a t g t g g t t t a t g g t c c c t t a c t a t a t g g t t g t g c c a c t t t g g t c t t t g a a g g g g t g t g a a t a c a c c a a a t a c c a g g g a a t g a t a t a c c a a c a c g g t g a a a c c a g a a a c t t c c c

4680 TPAYPNYSRYWDIIDEHKVT a c t c c t g c g t a c c c a a a t t a c t c c c g t t a t t g g g a t a t t a t t g a t g a a c a c a a a g t c a c c t g a g g a c g c a t g g g t t t a a t g a g g g c a a t a a c c c t a t a a t a a c t a c t t g t g t t t c a g t g g

4740 QFYVAPTALRLLKRAGDSYI c a a t t t t a t g t t g c c c c a a c t g c t t t g c g t t t g t t g a a a a g a g c t g g t g a t t c c t a c a t c g t t a a a a t a c a a c g g g g t t g a c g a a a c g c a a a c a a c t t t t c t c g a c c a c t a a g g a t g t a g

4800 ENHSLKSLRCLGSVGEPIAA g a a a a t c a t t c c t t a a a a t c t t t g c g t t g c t t g g g t t c g g t c g g t g a a c c a a t t g c t g c t c t t t t a g t a a g g a a t t t t a g a a a c g c a a c g a a c c c a a g c c a g c c a c t t g g t t a a c g a c g a

4860 EVWEWYSEKIGKNEIPIVDT g a a g t t t g g g a g t g g t a c t c t g a a a a a a t a g g t a a a a a t g a a a t c c c c a t t g t a g a c a c c c t t c a a a c c c t c a c c a t g a g a c t t t t t t a t c c a t t t t t a c t t t a g g g g t a a c a t c t g t g g

4920 YWQTESGSHLVTPLAGGVTP t a c t g g c a a a c a g a a t c t g g t t c g c a t c t g g t c a c c c c g c t g g c t g g t g g t g t c a c a c c a a t g a c c g t t t g t c t t a g a c c a a g c g t a g a c c a g t g g g g c g a c c g a c c a c c a c a g t g t g g t

4980 MKPGSASFPFFGIDAVVLDP a t g a a a c c g g g t t c t g c c t c a t t c c c c t t c t t c g g t a t t g a t g c a g t t g t t c t t g a c c c t t a c t t t g g c c c a a g a c g g a g t a a g g g g a a g a a g c c a t a a c t a c g t c a a c a a g a a c t g g g a

5040 NTGEELNTSHAEGVLAVKAA a a c a c t g g t g a a g a a c t t a a t a c c a g c c a c g c a g a g g g t g t c c t t g c c g t c a a a g c t g c a t t g t g a c c a c t t c t t g a a t t a t g g t c g g t g c g t c t c c c a c a g g a a c g g c a g t t t c g a c g t

5100 WPSFARTIWKNHDRYLDTYL t g g c c a t c a t t t g c a a g a a c t a t t t g g a a a a a t c a t g a t a g g t a t c t a g a c a c t t a t t t g a c c g g t a g t a a a c g t t c t t g a t a a a c c t t t t t a g t a c t a t c c a t a g a t c t g t g a a t a a a c

5160 NPYPGYYFTGDGAAKDKDGY a a c c c t t a c c c t g g c t a c t a t t t c a c t g g t g a t g g t g c t g c a a a g g a t a a g g a t g g t t a t t t g g g a a t g g g a c c g a t g a t a a a g t g a c c a c t a c c a c g a c g t t t c c t a t t c c t a c c a a t a

5220 IWILGRVDDVVNVSGHRLST a t c t g g a t t t t g g g t c g t g t a g a c g a t g t g g t g a a c g t c t c t g g t c a c c g t c t g t c t a c c t a g a c c t a a a a c c c a g c a c a t c t g c t a c a c c a c t t g c a g a g a c c a g t g g c a g a c a g a t g g

5280 AEIEAAIIEDPIVAECAVVG g c t g a a a t t g a g g c t g c t a t t a t c g a a g a t c c a a t t g t g g c c g a g t g t g c t g t t g t c g g a c g a c t t t a a c t c c g a c g a t a a t a g c t t c t a g g t t a a c a c c g g c t c a c a c g a c a a c a g c c t

5340 FNDDLTGQAVAAFVVLKNKS t t c a a c g a t g a c t t g a c t g g t c a a g c a g t t g c t g c a t t t g t g g t g t t g a a a a a c a a a t c t a a g t t g c t a c t g a a c t g a c c a g t t c g t c a a c g a c g t a a a c a c c a c a a c t t t t t g t t t a g a

5400 NWSTATDDELQDIKKHLVFT a a t t g g t c c a c c g c a a c a g a t g a t g a a t t a c a a g a t a t c a a g a a g c a t t t g g t c t t t a c t t t a a c c a g g t g g c g t t g t c t a c t a c t t a a t g t t c t a t a g t t c t t c g t a a a c c a g a a a t g a

5460 VRKDIGPFAAPKLIILVDDL g t t a g a a a a g a c a t c g g g c c a t t t g c c g c a c c a a a a t t g a t c a t t t t a g t g g a t g a c t t g c a a t c t t t t c t g t a g c c c g g t a a a c g g c g t g g t t t t a a c t a g t a a a a t c a c c t a c t g a a c

5520 PKTRSGKIMRRILRKILAGE c c c a a g a c a a g a t c t g g c a a a a t t a t g a g a c g t a t t t t a a g a a a a a t c c t a g c a g g a g a a g g g t t c t g t t c t a g a c c g t t t t a a t a c t c t g c a t a a a a t t c t t t t t a g g a t c g t c c t c t t

5580 SDQLGDVSTLSNPGIVRHPI a g t g a c c a a c t a g g c g a c g t t t c t a c a t t g t c a a a c c c t g g c a t t g t t a g a c a t c c a a t t t c a c t g g t t g a t c c g c t g c a a a g a t g t a a c a g t t t g g g a c c g t a a c a a t c t g t a g g t t a a

5640 1 5 EQKLI Myc DSVKLPRAAAAASFLEQKLI g a t t c g g t c a a g t t g c c t c g a g c c g c g g c g g c c g c c a g c t t t c t a g a a c a a a a a c t c a t c c t a a g c c a g t t c a a c g g a g c t c g g c g c c g c c g g c g g t c g a a a g a t c t t g t t t t t g a g t a g

5700 10 SEEDL (in frame with Myc) NSAVD 1 5 HHHHHH * Myc 6xHis SEEDLNSAVDHHHHHH* t c a g a a g a g g a t c t g a a t a g c g c c g t c g a c c a t c a t c a t c a t c a t c a t t g a g t t t g t a g c a g t c t t c t c c t a g a c t t a t c g c g g c a g c t g g t a g t a g t a g t a g t a g t a a c t c a a a c a t c g

5760 c t t a g a c a t g a c t g t t c c t c a g t t c a a g t t g g g c a c t t a c g a g a a g a c c g g t c t t g c t a g g a a t c t g t a c t g a c a a g g a g t c a a g t t c a a c c c g t g a a t g c t c t t c t g g c c a g a a c g a t c

5820 AOX1 terminator a t t c t a a t c a a g a g g a t g t c a g a a t g c c a t t t g c c t g a g a g a t g c a g g c t t c a t t t t t g a t a a g a t t a g t t c t c c t a c a g t c t t a c g g t a a a c g g a c t c t c t a c g t c c g a a g t a a a a a c t

5880 AOX1 terminator t a c t t t t t t a t t t g t a a c c t a t a t a g t a t a g g a t t t t t t t t g t c a t t t t g t t t c t t c t c g a t g a a a a a a t a a a c a t t g g a t a t a t c a t a t c c t a a a a a a a a c a g t a a a a c a a a g a a g a g c

5940 AOX1 terminator t a c g a g c t t g c t c c t g a t c a g c c t a t c t c g c a g c t g a t g a a t a t c t t g t g g t a g g g g t t t a t g c t c g a a c g a g g a c t a g t c g g a t a g a g c g t c g a c t a c t t a t a g a a c a c c a t c c c c a a a

6000 AOX1 terminator g g g a a a a t c a t t c g a g t t t g a t g t t t t t c t t g g t a t t t c c c a c t c c t c t t c a g a g t a c a g c c c t t t t a g t a a g c t c a a a c t a c a a a a a g a a c c a t a a a g g g t g a g g a g a a g t c t c a t g t c

6060 AOX1 terminator GAP promoter a a g a t t a a g t g a g a t t t t t g t a g a a a t g t c t t g g t g t c c t c g t c c a a t c a g g t a g c c a t c t t c t a a t t c a c t c t a a a a a c a t c t t t a c a g a a c c a c a g g a g c a g g t t a g t c c a t c g g t a g

6120 GAP promoter t c t g a a a t a t c t g g c t c c g t t g c a a c t c c g a a c g a c c t g c t g g c a a c g t a a a a t t c t c c g a g a c t t t a t a g a c c g a g g c a a c g t t g a g g c t t g c t g g a c g a c c g t t g c a t t t t a a g a g g c

6180 GAP promoter g g g t a a a a c t t a a a t g t g g a g t a a t g g a a c c a g a a a c g t c t c t t c c c t t c t c t c t c c t t c c c c a t t t t g a a t t t a c a c c t c a t t a c c t t g g t c t t t g c a g a g a a g g g a a g a g a g a g g a a g

6240 GAP promoter c a c c g c c c g t t a c c g t c c c t a g g a a a t t t t a c t c t g c t g g a g a g c t t c t t c t a c g g c c c c g t g g c g g g c a a t g g c a g g g a t c c t t t a a a a t g a g a c g a c c t c t c g a a g a a g a t g c c g g g g

6300 GAP promoter c t t g c a g c a a t g c t c t t c c c a g c a t t a c g t t g c g g g t a a a a c g g a g g t c g t g t a c c c g a c g a a c g t c g t t a c g a g a a g g g t c g t a a t g c a a c g c c c a t t t t g c c t c c a g c a c a t g g g c t g

6360 GAP promoter c t a g c a g c c c a g g g a t g g a a a a g t c c c g g c c g t c g c t g g c a a t a a t a g c g g g c g g a c g c a g a t c g t c g g g t c c c t a c c t t t t c a g g g c c g g c a g c g a c c g t t a t t a t c g c c c g c c t g c g t

6420 GAP promoter t g t c a t g a g a t t a t t g g a a a c c a c c a g a a t c g a a t a t a a a a g g c g a a c a c c t t t c c c a a t a c a g t a c t c t a a t a a c c t t t g g t g g t c t t a g c t t a t a t t t t c c g c t t g t g g a a a g g g t t a

6480 GAP promoter t t t g g t t t c t c c t g a c c c a a a g a c t t t a a a t t t a a t t t a t t t g t c c c t a t t t c a a t c a a t a a a c c a a a g a g g a c t g g g t t t c t g a a a t t t a a a t t a a a t a a a c a g g g a t a a a g t t a g t t a

6540 GAP promoter MTKLHFDTAEPVKITL t g a a c a a c t a t a t g a c t a a g c t a c a c t t t g a c a c t g c t g a a c c a g t c a a g a t c a c a c t t c a c t t g t t g a t a t a c t g a t t c g a t g t g a a a c t g t g a c g a c t t g g t c a g t t c t a g t g t g a a g

6600 PNGLTYEQPTGLFINNKFMK c a a a t g g t t t g a c a t a c g a g c a a c c a a c c g g t c t a t t c a t t a a c a a c a a g t t t a t g a a a g g t t t a c c a a a c t g t a t g c t c g t t g g t t g g c c a g a t a a g t a a t t g t t g t t c a a a t a c t t t c

6660 AQDGKTYPVEDPSTENTVCE c t c a a g a c g g t a a g a c c t a t c c c g t c g a a g a t c c t t c c a c t g a a a a c a c c g t t t g t g a g g g a g t t c t g c c a t t c t g g a t a g g g c a g c t t c t a g g a a g g t g a c t t t t g t g g c a a a c a c t c c

6720 VSSATTEDVEYAIECADRAF t c t c t t c t g c c a c c a c t g a a g a t g t t g a a t a t g c t a t c g a a t g t g c c g a c c g t g c t t t c c a g a g a a g a c g g t g g t g a c t t c t a c a a c t t a t a c g a t a g c t t a c a c g g c t g g c a c g a a a g g

6780 HDTEWATQDPRERGRLLSKL a c g a c a c t g a a t g g g c t a c c c a a g a c c c a a g a g a a a g a g g c c g t c t a c t a a g t a a g t t g g t g c t g t g a c t t a c c c g a t g g g t t c t g g g t t c t c t t t c t c c g g c a g a t g a t t c a t t c a a c c

6840 ADELESQIDLVSSIEALDNG c t g a c g a a t t g g a a a g c c a a a t t g a c t t g g t t t c t t c c a t t g a a g c t t t g g a c a a t g g t a g a c t g c t t a a c c t t t c g g t t t a a c t g a a c c a a a g a a g g t a a c t t c g a a a c c t g t t a c c a t

6900 KTLALARGDVTIAINCLRDA a a a c t t t g g c c t t a g c c c g t g g g g a t g t t a c c a t t g c a a t c a a c t g t c t a a g a g a t g c t g t t t g a a a c c g g a a t c g g g c a c c c c t a c a a t g g t a a c g t t a g t t g a c a g a t t c t c t a c g a c

6960 AAYADKVNGRTINTGDGYMN c t g c c t a t g c c g a c a a a g t c a a c g g t a g a a c a a t c a a c a c c g g t g a c g g c t a c a t g a a c t g a c g g a t a c g g c t g t t t c a g t t g c c a t c t t g t t a g t t g t g g c c a c t g c c g a t g t a c t t g a

7020 FTTLEPIGVCGQIIPWNFPI t c a c c a c c t t a g a g c c a a t c g g t g t c t g t g g t c a a a t t a t t c c a t g g a a c t t t c c a a t a a a g t g g t g g a a t c t c g g t t a g c c a c a g a c a c c a g t t t a a t a a g g t a c c t t g a a a g g t t a t t

7080 MMLAWKIAPALAMGNVCILK t g a t g t t g g c t t g g a a g a t c g c c c c a g c a t t g g c c a t g g g t a a c g t c t g t a t c t t g a a a c a c t a c a a c c g a a c c t t c t a g c g g g g t c g t a a c c g g t a c c c a t t g c a g a c a t a g a a c t t t g

7140 PAAVTPLNALYFASLCKKVG c c g c t g c t g t c a c a c c t t t a a a t g c c c t a t a c t t t g c t t c t t t a t g t a a g a a g g t t g g t a g g c g a c g a c a g t g t g g a a a t t t a c g g g a t a t g a a a c g a a g a a a t a c a t t c t t c c a a c c a t

7200 IPAGVVNIVPGPGRTVGAAL t t c c a g c t g g t g t c g t c a a c a t c g t t c c a g g t c c t g g t a g a a c t g t t g g t g c t g c t t t g a a a g g t c g a c c a c a g c a g t t g t a g c a a g g t c c a g g a c c a t c t t g a c a a c c a c g a c g a a a c t

7260 TNDPRIRKLAFTGSTEVGKS c c a a c g a c c c a a g a a t c a g a a a g c t g g c t t t t a c c g g t t c t a c a g a a g t c g g t a a g a g t g g g t t g c t g g g t t c t t a g t c t t t c g a c c g a a a a t g g c c a a g a t g t c t t c a g c c a t t c t c a c

7320 VAVDSSESNLKKITLELGGK t t g c t g t c g a c t c t t c t g a a t c t a a c t t g a a g a a a a t c a c t t t g g a a c t a g g t g g t a a g t a a c g a c a g c t g a g a a g a c t t a g a t t g a a c t t c t t t t a g t g a a a c c t t g a t c c a c c a t t c a

7380 SAHLVFDDANIKKTLPNLVN c c g c c c a t t t g g t c t t t g a c g a t g c t a a c a t t a a g a a g a c t t t a c c a a a t c t a g t a a a c g g g c g g g t a a a c c a g a a a c t g c t a c g a t t g t a a t t c t t c t g a a a t g g t t t a g a t c a t t t g c

7440 GIFKNAGQICSSGSRIYVQE g t a t t t t c a a g a a c g c t g g t c a a a t t t g t t c c t c t g g t t c t a g a a t t t a c g t t c a a g a a g c a t a a a a g t t c t t g c g a c c a g t t t a a a c a a g g a g a c c a a g a t c t t a a a t g c a a g t t c t t c

7500 GIYDELLAAFKAYLETEIKV g t a t t t a c g a c g a a c t a t t g g c t g c t t t c a a g g c t t a c t t g g a a a c c g a a a t c a a a g t t g c a t a a a t g c t g c t t g a t a a c c g a c g a a a g t t c c g a a t g a a c c t t t g g c t t t a g t t t c a a c

7560 GNPFDKANFQGAITNRQQFD g t a a t c c a t t t g a c a a g g c t a a c t t c c a a g g t g c t a t c a c t a a c c g t c a a c a a t t c g a c a c a t t a g g t a a a c t g t t c c g a t t g a a g g t t c c a c g a t a g t g a t t g g c a g t t g t t a a g c t g t

7620 TIMNYIDIGKKEGAKILTGG c a a t t a t g a a c t a c a t c g a t a t c g g t a a g a a a g a a g g c g c c a a g a t c t t a a c t g g t g g c g g t t a a t a c t t g a t g t a g c t a t a g c c a t t c t t t c t t c c g c g g t t c t a g a a t t g a c c a c c g c

7680 EKVGDKGYFIRPTVFYDVNE a a a a a g t t g g t g a c a a g g g t t a c t t c a t c a g a c c a a c c g t t t t c t a c g a t g t t a a t g a a g t t t t t c a a c c a c t g t t c c c a a t g a a g t a g t c t g g t t g g c a a a a g a t g c t a c a a t t a c t t c

7740 DMRIVKEEIFGPVVTVAKFK a c a t g a g a a t t g t t a a g g a a g a a a t t t t t g g a c c a g t t g t c a c t g t c g c a a a g t t c a a g a t g t a c t c t t a a c a a t t c c t t c t t t a a a a a c c t g g t c a a c a g t g a c a g c g t t t c a a g t t c t

EcoRI 7800 TLEEGVEMANSSEFGLGSGI c t t t a g a a g a a g g t g t c g a a a t g g c t a a c a g c t c t g a a t t c g g t c t a g g t t c t g g t a t c g g a a a t c t t c t t c c a c a g c t t t a c c g a t t g t c g a g a c t t a a g c c a g a t c c a a g a c c a t a g c

Acc65IKpnI 7860 ETESLSTGLKVAKMLKAGTV a a a c a g a a t c t t t g a g c a c a g g t t t g a a g g t g g c c a a g a t g t t g a a g g c c g g t a c c g t c t t t t g t c t t a g a a a c t c g t g t c c a a a c t t c c a c c g g t t c t a c a a c t t c c g g c c a t g g c a g a

7920 WINTYNDFDSRVPFGGVKQS g g a t c a a c a c a t a c a a c g a t t t t g a c t c c a g a g t t c c a t t c g g t g g t g t t a a g c a a t c t g c c t a g t t g t g t a t g t t g c t a a a a c t g a g g t c t c a a g g t a a g c c a c c a c a a t t c g t t a g a c

7980 GYGREMGEEVYHAYTEVKAV g t t a c g g t a g a g a a a t g g g t g a a g a a g t c t a c c a t g c a t a c a c t g a a g t a a a a g c t g t c a c a a t g c c a t c t c t t t a c c c a c t t c t t c a g a t g g t a c g t a t g t g a c t t c a t t t t c g a c a g t

8040 RIKL* g a a t t a a g t t g t a a c c t a g g g c g g c c g c g a a t t a a t t c g c c t t a g a c a t g a c t g t t c c t c c t t a a t t c a a c a t t g g a t c c c g c c g g c g c t t a a t t a a g c g g a a t c t g t a c t g a c a a g g a g

8100 AOX1 terminator a g t t c a a g t t g g g c a c t t a c g a g a a g a c c g g t c t t g c t a g a t t c t a a t c a a g a g g a t g t c t c a a g t t c a a c c c g t g a a t g c t c t t c t g g c c a g a a c g a t c t a a g a t t a g t t c t c c t a c a g

8160 AOX1 terminator a g a a t g c c a t t t g c c t g a g a g a t g c a g g c t t c a t t t t t g a t a c t t t t t t a t t t g t a a c c t t c t t a c g g t a a a c g g a c t c t c t a c g t c c g a a g t a a a a a c t a t g a a a a a a t a a a c a t t g g a

8220 AOX1 terminator a t a t a g t a t a g g a t t t t t t t t g t c a t t t t g t t t c t t c t c g t a c g a g c t t g c t c c t g a t c a t a t a t c a t a t c c t a a a a a a a a c a g t a a a a c a a a g a a g a g c a t g c t c g a a c g a g g a c t a g t

8280 AOX1 terminator g c c t a t c t c g c a g c t g a t g a a t a t c t t g t g g t a g g g g t t t g g g a a a a t c a t t c g a g t t t g c g g a t a g a g c g t c g a c t a c t t a t a g a a c a c c a t c c c c a a a c c c t t t t a g t a a g c t c a a a c

8340 AOX1 terminator a t g t t t t t c t t g g t a t t t c c c a c t c c t c t t c a g a g t a c a g a a g a t t a a g t g a g a c g t t c g t a c a a a a a g a a c c a t a a a g g g t g a g g a g a a g t c t c a t g t c t t c t a a t t c a c t c t g c a a g c

8400 t t t g t g c a a g c t t a t c g a t a a g c t t t a a t g c g g t a g t t t a t c a c a g t t a a a t t g c t a a c g a a a c a c g t t c g a a t a g c t a t t c g a a a t t a c g c c a t c a a a t a g t g t c a a t t t a a c g a t t g c

8460 c a g t c a g g c a c c g t g t a t g a a a t c t a a c a a t g c g c t c a t c g t c a t c c t c g g c a c c g t c a c g t c a g t c c g t g g c a c a t a c t t t a g a t t g t t a c g c g a g t a g c a g t a g g a g c c g t g g c a g t g

8520 c c t g g a t g c t g t a g g c a t a g g c t t g g t t a t g c c g g t a c t g c c g g g c c t c t t g c g g g a t a t g g a c c t a c g a c a t c c g t a t c c g a a c c a a t a c g g c c a t g a c g g c c c g g a g a a c g c c c t a t a

BmtINheI 8580 c g t c c a t t c c g a c a g c a t c g c c a g t c a c t a t g g c g t g c t g c t a g c g c t a t a t g c g t t g a t g c a g g t a a g g c t g t c g t a g c g g t c a g t g a t a c c g c a c g a c g a t c g c g a t a t a c g c a a c t a

FspAI 8640 g c a a t t t c t a t g c g c a c c c g t t c t c g g a g c a c t g t c c g a c c g c t t t g g c c g c c g c c c a g t c g t t a a a g a t a c g c g t g g g c a a g a g c c t c g t g a c a g g c t g g c g a a a c c g g c g g c g g g t c a

8700 c c t g c t c g c t t c g c t a c t t g g a g c c a c t a t c g a c t a c g c g a t c a t g g c g a c c a c a c c c g t g g a c g a g c g a a g c g a t g a a c c t c g g t g a t a g c t g a t g c g c t a g t a c c g c t g g t g t g g g c a

8760 c c t g t g g a t c t a t c g a a t c t a a a t g t a a g t t a a a a t c t c t a a a t a a a c t t g a a g t c g g a c g g a c a c c t a g a t a g c t t a g a t t t a c a t t c a a t t t t a g a g a t t t a t t t g a a c t t c a g c c t g

8820 a g t g a g t g t a g t c t t g a g a a a t t c t g a a g c c g t a t t t t t a t t a t c a g t g a g t c a g t c a t c t c a c t c a c a t c a g a a c t c t t t a a g a c t t c g g c a t a a a a a t a a t a g t c a c t c a g t c a g t a g

BbvCI 8880 a g g a g a t c c t c t a c g c c g g a c g c a t c g t g g c c g a c c t g c a g g g g g g g g g g g g c g c t g a g g t c c t c t a g g a g a t g c g g c c t g c g t a g c a c c g g c t g g a c g t c c c c c c c c c c c c g c g a c t c c

StuI* 8940 t c t g c c t c g t g a a g a a g g t g t t g c t g a c t c a t a c c a g g c c t g a a t c g c c c c a t c a t c c a g a g a c g g a g c a c t t c t t c c a c a a c g a c t g a g t a t g g t c c g g a c t t a g c g g g g t a g t a g g t c

9000 c c a g a a a g t g a g g g a g c c a c g g t t g a t g a g a g c t t t g t t g t a g g t g g a c c a g t t g g t g a t g g t c t t t c a c t c c c t c g g t g c c a a c t a c t c t c g a a a c a a c a t c c a c c t g g t c a a c c a c t a

9060 t t t g a a c t t t t g c t t t g c c a c g g a a c g g t c t g c g t t g t c g g g a a g a t g c g t g a t c t g a t c a a a c t t g a a a a c g a a a c g g t g c c t t g c c a g a c g c a a c a g c c c t t c t a c g c a c t a g a c t a g

9120 c t t c a a c t c a g c a a a a g t t c g a t t t a t t c a a c a a a g c c g c c g t c c c g t c a a g t c a g c g t a g a a g t t g a g t c g t t t t c a a g c t a a a t a a g t t g t t t c g g c g g c a g g g c a g t t c a g t c g c a t

9180 270 *FFEDLM KanR 270 *FFEDLM KanR *FFEDLM a t g c t c t g c c a g t g t t a c a a c c a a t t a a c c a a t t c t g a t t a g a a a a a c t c a t c g a g c a t c t a c g a g a c g g t c a c a a t g t t g g t t a a t t g g t t a a g a c t a a t c t t t t t g a g t a g c t c g t a g

9240 265 260 255 250 LHFQLKNMDPNDIGYKQFLR KanR 265 260 255 250 LHFQLKNMDPNDIGYKQFLR KanR LHFQLKNMDPNDIGYKQFLR a a a t g a a a c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t t a c t t t g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a

9300 245 240 235 230 KQLSPSFEGLCNWLIALDQY KanR 245 240 235 230 KQLSPSFEGLCNWLIALDQY KanR KQLSPSFEGLCNWLIALDQY t t c t g t a a t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t a a g a c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a

9360 225 220 215 210 RDAIGVRGVDICGILKGEDF KanR 225 220 215 210 RDAIGVRGVDICGILKGEDF KanR RDAIGVRGVDICGILKGEDF c g g t c t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a g c c a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t

9420 205 200 195 190 ILNDLSFDGHTVVSDPSFPL KanR 205 200 195 190 ILNDLSFDGHTVVSDPSFPL KanR ILNDLSFDGHTVVSDPSFPL a t a a g g t t a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a t a t t c c a a t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t

9480 185 180 175 170 LKHMEKWVQEVPWGNREDDF KanR 185 180 175 170 LKHMEKWVQEVPWGNREDDF KanR LKHMEKWVQEVPWGNREDDF a g c t t a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c g a a t a c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t

9540 165 160 155 150 DSADVLGNNMRSQAQALRFV KanR 165 160 155 150 DSADVLGNNMRSQAQALRFV KanR DSADVLGNNMRSQAQALRFV t c a c t c g c a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g a g t g a g c g t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c

AsiSI 9600 145 140 135 130 RDSNFPCNCVPISHLRRLFV KanR 145 140 135 130 RDSNFPCNCVPISHLRRLFV KanR RDSNFPCNCVPISHLRRLFV c g a t c g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c t a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a

9660 125 120 115 110 ALADVINEGSDPYEELVQFA KanR 125 120 115 110 ALADVINEGSDPYEELVQFA KanR ALADVINEGSDPYEELVQFA g c c a g c g c a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t c g g t c g c g t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a

TspMIXmaISmaI 9720 105 100 95 90 TKGPIATTLLWADDPTRIFH KanR 105 100 95 90 TKGPIATTLLWADDPTRIFH KanR TKGPIATTLLWADDPTRIFH g t t t t c c c g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c c a a a a g g g c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g

9780 85 80 75 70 KITPLPMFETLWNLRVMEDT KanR 85 80 75 70 KITPLPMFETLWNLRVMEDT KanR KITPLPMFETLWNLRVMEDT t t g a t g g t c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a a c t a c c a g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t

9840 65 60 55 50 VDNAVSGKGHKLFLEPADPK KanR 65 60 55 50 VDNAVSGKGHKLFLEPADPK KanR VDNAVSGKGHKLFLEPADPK a c a t c a t t g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c t g t a g t a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g

NruI 9900 45 40 35 30 GYLRYITAGSQGVNDRAWKY KanR 45 40 35 30 GYLRYITAGSQGVNDRAWKY KanR A T C G C G A G C C C A T T T A T A C Kan-R GYLRYITAGSQGVNDRAWKY c c a t a c a a t c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c g g t a t g t t a g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g

9960 25 20 15 10 GYLDADMNSNLRPRSCSTER KanR 25 20 15 10 GYLDADMNSNLRPRSCSTER KanR C Kan-R GYLDADMNSNLRPRSCSTER c c a t a t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t c g a g c a a g a c g t t t c c c g t g g t a t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a g c t c g t t c t g c a a a g g g c a

10,020 5 1 QIHSM KanR 5 1 QIHSM KanR A T G G C T C A T A A C A C C C C T T G pENTR-R QIHSM t g a a t a t g g c t c a t a a c a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t a c t t a t a c c g a g t a t t g t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a

DraIII 10,080 c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a c a a c g t g g g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t g t g t t g c a c c

10,140 G G pBRrevBam c t t t c c c c c c c c c c c c t g c a g g t c g g c a t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c g a a a g g g g g g g g g g g g a c g t c c a g c c g t a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g

10,200 A T A T A G C G G C T G T A G T G G pBRrevBam *IASMVSPSSRARWKPSMLA t a t a t c g c c g a c a t c a c c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t a t a t a g c g g c t g t a g t g g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a

10,260 QKPTPITAPGTAPPSNPAME t g t t t c g g c g t g g g t a t g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c a c a a a g c c g c a c c c a t a c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g

SphI 10,320 KCAGNRAAATSLPRLRSSPQ t t g c a t g c a c c a t t c c t t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c a a c g t a c g t g g t a a g g a a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g

10,380 AOX1 3' fragment KRICSDCLPSRRTDIIPLIP t t c c t a a t g c a g g a g t c g c a t a a g g g a g a g c g t c g a g t a t c t a t g a t t g g a a g t a t g g g a a a g g a t t a c g t c c t c a g c g t a t t c c c t c t c g c a g c t c a t a g a t a c t a a c c t t c a t a c c c t

10,440 AOX1 3' fragment ITIGANKLTKLDGILNHGVL a t g g t g a t a c c c g c a t t c t t c a g t g t c t t g a g g t c t c c t a t c a g a t t a t g c c c a a c t a a a t a c c a c t a t g g g c g t a a g a a g t c a c a g a a c t c c a g a g g a t a g t c t a a t a c g g g t t g a t t t

10,500 AOX1 3' fragment AVPPPSKMTFKESKQDDTSE g c a a c c g g a g g a g g a g a t t t c a t g g t a a a t t t c t c t g a c t t t t g g t c a t c a g t a g a c t c g c g t t g g c c t c c t c c t c t a a a g t a c c a t t t a a a g a g a c t g a a a a c c a g t a g t c a t c t g a g c

10,560 AOX1 3' fragment FQSVIETIVASIDKKSVTFS a a c t g t g a g a c t a t c t c g g t t a t g a c a g c a g a a a t g t c c t t c t t g g a g a c a g t a a a t g a a t t g a c a c t c t g a t a g a g c c a a t a c t g t c g t c t t t a c a g g a a g a a c c t c t g t c a t t t a c t t

10,620 AOX1 3' fragment TGGIFFDKNDPVFKKNRVKE g t c c c a c c a a t a a a g a a a t c c t t g t t a t c a g g a a c a a a c t t c t t g t t t c g a a c t t t t t c g c a g g g t g g t t a t t t c t t t a g g a a c a a t a g t c c t t g t t t g a a g a a c a a a g c t t g a a a a a g c

10,680 AOX1 3' fragment TGQVIFHLTSIDPLFPAPLH g t g c c t t g a a c t a t a a a a t g t a g a g t g g a t a t g t c g g g t a g g a a t g g a g c g g g c a a a t g c c a c g g a a c t t g a t a t t t t a c a t c t c a c c t a t a c a g c c c a t c c t t a c c t c g c c c g t t t a c g

10,740 AOX1 3' fragment KGEPGELPIYPKYISIGVET t t a c c t t c t g g a c c t t c a a g a g g t a t g t a g g g t t t g t a g a t a c t g a t g c c a a c t t c a g t g a a t g g a a g a c c t g g a a g t t c t c c a t a c a t c c c a a a c a t c t a t g a c t a c g g t t g a a g t c a c

10,800 AOX1 3' fragment VVNSNREFWESDLSILTTQR a c a a c g t t g c t a t t t c g t t c a a a c c a t t c c g a a t c c a g a g a a a t c a a a g t t g t t t g t c t a t g t t g c a a c g a t a a a g c a a g t t t g g t a a g g c t t a g g t c t c t t t a g t t t c a a c a a a c a g a t

10,860 AOX1 3' fragment SNIWALATKFSVITHEHKST c t a t t g a t c c a a g c c a g t g c g g t c t t g a a a c t g a c a a t a g t g t g c t c g t g t t t t g a g g t c g a t a a c t a g g t t c g g t c a c g c c a g a a c t t t g a c t g t t a t c a c a c g a g c a c a a a a c t c c a g

10,920 AOX1 3' fragment MKTHIFRTKSRFLRSSGSSL a t c t t t g t a t g a a t a a a t c t a g t c t t t g a t c t a a a t a a t c t t g a c g a g c c a g a c g a t a a t t a g a a a c a t a c t t a t t t a g a t c a g a a a c t a g a t t t a t t a g a a c t g c t c g g t c t g c t a t t a

10,980 AOX1 3' fragment VLRFEKFTLPCTHRGTNFGL a c c a a t c t a a a c t c t t t a a a c g t t a a a g g a c a a g t a t g t c t g c c t g t a t t a a a c c c c a a a t g g t t a g a t t t g a g a a a t t t g c a a t t t c c t g t t c a t a c a g a c g g a c a t a a t t t g g g g t t t

11,040 AOX1 3' fragment DARLRIRM t c a g c t c g t a g t c t g a t c c t c a t c a a c t t g a g g g g c a c t a t c t t g t t t t a g a g a a a t t t g a g t c g a g c a t c a g a c t a g g a g t a g t t g a a c t c c c c g t g a t a g a a c a a a a t c t c t t t a a a c

11,100 AOX1 3' fragment c g g a g a t g c g a t a t c g a g a a a a a g g t a c g c t g a t t t t a a a c g t g a a a t t t a t c t c a t g a t g c c t c t a c g c t a t a g c t c t t t t t c c a t g c g a c t a a a a t t t g c a c t t t a a a t a g a g t a c t a

11,160 AOX1 3' fragment G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' c t c t g c c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g g a g a c g g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c

11,220 a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t

11,280 bom g c g g g t g t t g g c g g g t g t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g c g c c c a c a a c c g c c c a c a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c

NdeI 11,340 bom C G G C A T C A G A G C A G A T T G T A pRS-marker t a t a c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t a t a t g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a

11,400 bom G A L4440 g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c t c t t c c g c t t c c t c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g a g a a g g c g a a g g a

11,460 G C G A G T G A C T G A G C G A L4440 c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t

PciI 11,520 a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t

11,580 ori a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g

11,640 ori t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t

11,700 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g

11,760 ori c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a

11,820 ori c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a

11,880 ori g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c

11,940 ori a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t

12,000 ori g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a

12,060 ori a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t

12,120 ori g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a

12,180 ori g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t

12,240 c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a

12,300 c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t

12,360 285 280 *WHKILSAGIE AmpR *WHKILSAGIE g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a

12,420 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t

12,480 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a

12,540 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c

12,600 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t

12,660 195 190 185 180 LEGTLLKRLTTAMAAPMTTD AmpR LEGTLLKRLTTAMAAPMTTD g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t g c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a c g t c c g t a g c a c c a c a

12,720 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t

12,780 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t

12,840 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t

12,900 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a

12,960 95 90 85 80 SYHIRRGLQEQGADVRSLVA AmpR A T A A T A C C G Amp-R SYHIRRGLQEQGADVRSLVA g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a c a c g g g a t a a t a c c g c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t g t g c c c t a t t a t g g c

13,020 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR C G C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g

13,080 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a

13,140 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t

13,200 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a

13,260 AmpR promoter t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a

13,320 AmpR promoter g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c

ZraIAatII 13,380 A A T A G G C G T A T C A C G A G G C pBRforEco a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g

3ʹ 5ʹ 13,406 c c t t t c g t c t t c a a g a a t t a a t t c t c g g a a a g c a g a a g t t c t t a a t t a a g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.