Addgene TRBV24-1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: TRBV24-1 Sequencing Result


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BtgZI (3402) BsrDI (3383) pENTR-R (3297 .. 3316) Kan-R (3219 .. 3238) EcoNI (2966) SspI (2954) AsiSI (2881) Bpu10I (2859) PflMI (2619) AcuI (2217) AlwNI (2085) BssSI - BssSαI (1842) pBR322ori-F (1810 .. 1829) PfoI (46) pGEX 3' (29 .. 51) pRS-marker (151 .. 170) NdeI (184) BstAPI (185) KasI (235) BsaHI - NarI (236) SfoI (237) PluTI (239) FspI (258) M13/pUC Forward (364 .. 386) EcoRI (396) Eco53kI (404) SacI (406) Acc65I (408) KpnI (412) XbaI (425) MslI (427) BsaBI * (433) AgeI (440) EcoO109I (478) BglII (685) Bsu36I (782) BpmI (1036) BbsI (1080) BseRI (1181) EagI - NotI (1252) AvaI - BsoBI - TspMI - XmaI (1278) SmaI (1280) PspOMI (1281) ApaI (1285) PstI (1296) StuI (1300) SphI (1308) M13 rev M13 Reverse (1328 .. 1344) lac operator M13/pUC Reverse (1341 .. 1363) L4440 (1559 .. 1576) AflIII - PciI (1669) TRBV24-1 3418 bp

Sequence View

PfoI 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t

120 c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c

180 C G G C A T C A G A G C A G A T T G T A pRS-marker t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g

KasIBsaHINarISfoIPluTINdeIBstAPI 240 MRCEIPHRCVRRKYRIRR a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c c t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g g

FspI 300 HSPFRLRNCWEGRSVRASSL a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a

360 LRQLAKGGCAARRLSWVTPG t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a

EcoRIEco53kISacIAcc65IKpnI 420 C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward FSQSRRCKTTAREFELGTSR t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g a g a a t t c g a g c t c g g t a c c t c g c g a a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c t c t t a a g c t c g a g c c a t g g a g c g c t t

EcoO109IAgeIBsaBI*XbaIMslI 480 Kozak sequence IHLDGDPPVATMASLLFFCG t a c a t c t a g a t g g g g a t c c a c c g g t c g c c a c c a t g g c c t c c c t g c t c t t c t t c t g t g g g g a t g t a g a t c t a c c c c t a g g t g g c c a g c g g t g g t a c c g g a g g g a c g a g a a g a a g a c a c c c c

540 AFYLLGTGSMDADVTQTPRN c c t t t t a t c t c c t g g g a a c a g g g t c c a t g g a t g c t g a t g t t a c c c a g a c c c c a a g g a a t a g g a a a a t a g a g g a c c c t t g t c c c a g g t a c c t a c g a c t a c a a t g g g t c t g g g g t t c c t t a t

600 RITKTGKRIMLECSQTKGHD g g a t c a c a a a g a c a g g a a a g a g g a t t a t g c t g g a a t g t t c t c a g a c t a a g g g t c a t g a t a c c t a g t g t t t c t g t c c t t t c t c c t a a t a c g a c c t t a c a a g a g t c t g a t t c c c a g t a c t a t

660 RMYWYRQDPGLGLRLIYYSF g a a t g t a c t g g t a t c g a c a a g a c c c a g g a c t g g g c c t a c g g t t g a t c t a t t a c t c c t t t g c t t a c a t g a c c a t a g c t g t t c t g g g t c c t g a c c c g g a t g c c a a c t a g a t a a t g a g g a a a c

BglII 720 DVKDINKGEISDGYSVSRQA a t g t c a a a g a t a t a a a c a a a g g a g a g a t c t c t g a t g g a t a c a g t g t c t c t c g a c a g g c a c t a c a g t t t c t a t a t t t g t t t c c t c t c t a g a g a c t a c c t a t g t c a c a g a g a g c t g t c c g t g

780 QAKFSLSLESAIPNQTALYE a g g c t a a a t t c t c c c t g t c c c t a g a g t c t g c c a t c c c c a a c c a g a c a g c t c t t t a c g a g a t c c g a t t t a a g a g g g a c a g g g a t c t c a g a c g g t a g g g g t t g g t c t g t c g a g a a a t g c t c t

Bsu36I 840 T* c c t a a g g t g g t g g t g g t t c t g g t t c t g g t t c t g g t g g t g g t t c t g g t g g t g g t c t c t t g a g g a t t c c a c c a c c a c c a a g a c c a a g a c c a a g a c c a c c a c c a a g a c c a c c a c c a g a g a a c t

900 c c c t g c c g t g t a c c a g c t g a g a g a c t c t a a a t c c a g t g a c a a g t c t g t c t g c c t a t t c a c g g g a c g g c a c a t g g t c g a c t c t c t g a g a t t t a g g t c a c t g t t c a g a c a g a c g g a t a a g t g

960 c g a t t t t g a t t c t c a a a c a a a t g t g t c a c a a a g t a a g g a t t c t g a t g t g t a t a t c a c a g a g c t a a a a c t a a g a g t t t g t t t a c a c a g t g t t t c a t t c c t a a g a c t a c a c a t a t a g t g t c t

1020 c a a a a c t g t g c t a g a c a t g a g g t c t a t g g a c t t c a a g a g c a a c a g t g c t g t g g c c t g g a g g t t t t g a c a c g a t c t g t a c t c c a g a t a c c t g a a g t t c t c g t t g t c a c g a c a c c g g a c c t c

BpmI 1080 c a a c a a a t c t g a c t t t g c a t g t g c a a a c g c c t t c a a c a a c a g c a t t a t t c c a g a a g a c a c g t t g t t t a g a c t g a a a c g t a c a c g t t t g c g g a a g t t g t t g t c g t a a t a a g g t c t t c t g t g

BbsI 1140 c t t c t t c c c c a g c c c a g a a a g t t c c t g t g a t g t c a a g c t g g t c g a g a a a a g c t t t g a a a c g a a g a a g g g g t c g g g t c t t t c a a g g a c a c t a c a g t t c g a c c a g c t c t t t t c g a a a c t t t g

BseRI 1200 a g a t a c g a a c c t a a a c t t t c a a a a c c t g t c a g t g a t t g g g t t c c g a a t c c t c c t c c t g a a t c t a t g c t t g g a t t t g a a a g t t t t g g a c a g t c a c t a a c c c a a g g c t t a g g a g g a g g a c t t

EagINotI 1260 a g t g g c c g g g t t t a a t c t g c t c a t g a c g c t g c g g c t g t g g t c c a g c t a a a g c g g c c g c g t t c a c c g g c c c a a a t t a g a c g a g t a c t g c g a c g c c g a c a c c a g g t c g a t t t c g c c g g c g c a

SphIStuIPstITspMIBsoBIXmaIAvaISmaIPspOMIApaI 1320 c g a c a a t c a a a t c g g a t c c c g g g c c c g t c g a c t g c a g a g g c c t g c a t g c a a g c t t g g t g t g c t g t t a g t t t a g c c t a g g g c c c g g g c a g c t g a c g t c t c c g g a c g t a c g t t c g a a c c a c a

1380 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t

1440 -35 lac promoter CAP binding site t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a

1500 CAP binding site t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a

1560 G A L4440 a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a

1620 G C G A G T G A C T G A G C G A L4440 c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t

AflIIIPciI 1680 a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t

1740 ori a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g

1800 ori t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t

BssSαIBssSI 1860 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g

1920 ori c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a

1980 ori c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a

2040 ori g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c

AlwNI 2100 ori a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t

2160 ori g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a

AcuI 2220 ori a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t

2280 ori g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a

2340 ori g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t

2400 c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a

2460 c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a g c c c a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t c g g g t t

2520 270 265 *FFEDLML KanR *FFEDLML t c t g a a t a a t g t t a c a a c c a a t t a a c c a a t t c t g a t t a g a a a a a c t c a t c g a g c a t c a a a a g a c t t a t t a c a a t g t t g g t t a a t t g g t t a a g a c t a a t c t t t t t g a g t a g c t c g t a g t t t

2580 260 255 250 245 HFQLKNMDPNDIGYKQFLRK KanR HFQLKNMDPNDIGYKQFLRK t g a a a c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c a c t t t g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g

PflMI 2640 240 235 230 225 QLSPSFEGLCNWLIALDQYR KanR QLSPSFEGLCNWLIALDQYR t g t a a t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g a c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c

2700 220 215 210 205 DAIGVRGVDICGILKGEDFI KanR DAIGVRGVDICGILKGEDFI t c t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t

2760 200 195 190 185 LNDLSFDGHTVVSDPSFPLL KanR LNDLSFDGHTVVSDPSFPLL a g g t t a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t c c a a t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a

2820 180 175 170 165 KHMEKWVQEVPWGNREDDFD KanR KHMEKWVQEVPWGNREDDFD t t a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a a a t a c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t

Bpu10I 2880 160 155 150 145 SADVLGNNMRSQAQALRFVR KanR SADVLGNNMRSQAQALRFVR c t c g c a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a g a g c g t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t

AsiSI 2940 140 135 130 125 DSNFPCNCVPISHLRRLFVA KanR DSNFPCNCVPISHLRRLFVA t c g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g

EcoNISspI 3000 120 115 110 105 LADVINEGSDPYEELVQFAT KanR LADVINEGSDPYEELVQFAT a g c g c a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t c g c g t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a

3060 100 95 90 85 KGPIATTLLWADDPTRIFHK KanR KGPIATTLLWADDPTRIFHK t t t c c g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a a a g g c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c

3120 80 75 70 65 ITPLPMFETLWNLRVMEDTV KanR ITPLPMFETLWNLRVMEDTV a t g g t c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t a c c a g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t

3180 60 55 50 45 DNAVSGKGHKLFLEPADPKG KanR DNAVSGKGHKLFLEPADPKG t c a t t g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a a g t a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t

3240 40 35 30 25 YLRYITAGSQGVNDRAWKYG KanR A T C G C G A G C C C A T T T A T A C C Kan-R YLRYITAGSQGVNDRAWKYG t a c a a g c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a a t g t t c g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t

3300 20 15 10 5 YLDADMNSNLRPRSTERQIH KanR A T G G pENTR-R YLDADMNSNLRPRSTERQIH t a t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t c g a c g t t t c c c g t t g a a t a t g g a t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a g c t g c a a a g g g c a a c t t a t a c c

3360 1 SM KanR C T C A T A A C A C C C C T T G pENTR-R SM c t c a t a a c a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a t g a t g a g t a t t g t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t a c t a

BtgZIBsrDI 3418 a t a t t t t t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a c g g g c c a g a g c t g c a t a t a a a a a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t g t g c c c g g t c t c g a c g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.