Addgene OmpA-mNG-mCh Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: OmpA-mNG-mCh Sequencing Result


Map View

M13/pUC Reverse (95 .. 117) EcoRV (5749) ApaI (5510) PspOMI (5506) BclI * (5313) MluI (5299) LacI-R (4968 .. 4987) NsiI (4862) MauBI (4838) pGEX 3' (4771 .. 4793) PfoI (4770) PflFI - Tth111I (4671) BstZ17I (4646) AccI (4645) pRS-marker (4611 .. 4630) NdeI (4594) BspQI - SapI (4532) L4440 (4513 .. 4530) PciI (4415) pBR322ori-F (4260 .. 4279) FspI (3304) PvuI (3158) lac operator BtgI - NcoI (125) NruI (148) BglII (278) BlpI (721) BamHI (774) AfeI (1318) EcoRI (1501) mCherry-R (1652 .. 1670) PstI - SbfI (1862) mCherry-F (1920 .. 1939) BbvCI (2045) AleI (2149) HindIII (2216) pBAD Reverse (2254 .. 2271) Amp-R (2958 .. 2977) ScaI (3046) OmpA-mNG-mCh 6210 bp

Sequence View

5ʹ 3ʹ 60 -35 trc promoter g t t t t t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c t g t t t a c a a t c a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g a c a a a t g t t a

120 -10 trc promoter lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse t a a t c a t c c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a a t t a g t a g g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t

NruINcoIBtgI 180 5 10 15 KKTAIAIAVALAGFAT OmpA signal peptide MAKKTAIAIAVALAGFAT c a g a c c a t g g c a a a a a a g a c a g c t a t c g c g a t t g c a g t g g c a c t g g c t g g t t t c g c t a c c g t c t g g t a c c g t t t t t t c t g t c g a t a g c g c t a a c g t c a c c g t g a c c g a c c a a a g c g a t g g

240 20 VAQA (in frame with OmpA signal peptide) APKDNTWYTGAKLGWS OmpA signal peptide VAQAAPKDNTWYTGAKLGWS g t a g c g c a g g c c g c t c c g a a a g a t a a c a c c t g g t a c a c t g g t g c t a a a c t g g g c t g g t c c c a t c g c g t c c g g c g a g g c t t t c t a t t g t g g a c c a t g t g a c c a c g a t t t g a c c c g a c c a g g

BglII 300 (in frame with OmpA signal peptide) QYHDTGLIGQRLEICQNVCY QYHDTGLIGQRLEICQNVCY c a g t a c c a t g a c a c t g g c c t c a t c g g c c a a c g c t t g g a g a t c t g c c a g a a c g t t t g c t a c g t c a t g g t a c t g t g a c c g g a g t a g c c g g t t g c g a a c c t c t a g a c g g t c t t g c a a a c g a t g

360 (in frame with OmpA signal peptide) YLGTLNGPTHENQLGAGAFG YLGTLNGPTHENQLGAGAFG t a c c t c g g g a c g c t c a a t g g t c c g a c c c a t g a a a a c c a a c t g g g c g c t g g t g c t t t t g g t a t g g a g c c c t g c g a g t t a c c a g g c t g g g t a c t t t t g g t t g a c c c g c g a c c a c g a a a a c c a

420 (in frame with OmpA signal peptide) GYQVNPYVGFEMGYDWLGRM GYQVNPYVGFEMGYDWLGRM g g t t a c c a g g t t a a c c c g t a t g t t g g c t t t g a a a t g g g t t a c g a c t g g t t a g g t c g t a t g c c a a t g g t c c a a t t g g g c a t a c a a c c g a a a c t t t a c c c a a t g c t g a c c a a t c c a g c a t a c

480 (in frame with OmpA signal peptide) PYKGSVENGAYKAQGVQLTA PYKGSVENGAYKAQGVQLTA c c g t a c a a a g g c a g c g t t g a a a a c g g t g c a t a c a a a g c t c a g g g c g t t c a a c t g a c c g c t g g c a t g t t t c c g t c g c a a c t t t t g c c a c g t a t g t t t c g a g t c c c g c a a g t t g a c t g g c g a

540 (in frame with OmpA signal peptide) KLGYPITDDLDIYTRLGGMV KLGYPITDDLDIYTRLGGMV a a a c t g g g t t a c c c a a t c a c t g a c g a c c t g g a c a t c t a c a c t c g t c t g g g t g g c a t g g t a t t t g a c c c a a t g g g t t a g t g a c t g c t g g a c c t g t a g a t g t g a g c a g a c c c a c c g t a c c a t

600 (in frame with OmpA signal peptide) WRADTKSNVYGKNHDTGVSP WRADTKSNVYGKNHDTGVSP t g g c g t g c a g a c a c t a a a t c c a a c g t t t a t g g t a a a a a c c a c g a c a c c g g c g t t t c t c c g a c c g c a c g t c t g t g a t t t a g g t t g c a a a t a c c a t t t t t g g t g c t g t g g c c g c a a a g a g g c

660 (in frame with OmpA signal peptide) VFAGGVEYAITPEIATRLEY VFAGGVEYAITPEIATRLEY g t c t t c g c t g g c g g t g t t g a g t a c g c g a t c a c t c c t g a a a t c g c t a c c c g t c t g g a a t a c c a g a a g c g a c c g c c a c a a c t c a t g c g c t a g t g a g g a c t t t a g c g a t g g g c a g a c c t t a t g

720 (in frame with OmpA signal peptide) QWTNNIGDAGQIGQRPDNGM QWTNNIGDAGQIGQRPDNGM c a g t g g a c c a a c a a c a t c g g t g a c g c a g g c c a g a t c g g c c a g c g t c c g g a c a a c g g c a t g g t c a c c t g g t t g t t g t a g c c a c t g c g t c c g g t c t a g c c g g t c g c a g g c c t g t t g c c g t a c

BamHIBlpI 780 (in frame with OmpA signal peptide) LSLGVSYRFGQGEAAPLEDP LSLGVSYRFGQGEAAPLEDP c t g a g c c t g g g t g t t t c c t a c c g t t t c g g t c a g g g c g a a g c a g c t c c a c t t g a g g a t c c c g a c t c g g a c c c a c a a a g g a t g g c a a a g c c a g t c c c g c t t c g t c g a g g t g a a c t c c t a g g g

840 PAEL 1 5 10 15 MVSKGEEDNMASLPAT mNeonGreen PAELMVSKGEEDNMASLPAT c c c g c t g a a t t g a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c t c t c t c c c a g c g a c a g g g c g a c t t a a c t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g a g a g a g g g t c g c t g t

900 20 25 30 35 HELHIFGSINGVDFDMVGQG mNeonGreen HELHIFGSINGVDFDMVGQG c a t g a g t t a c a c a t c t t t g g c t c c a t c a a c g g t g t g g a c t t t g a c a t g g t g g g t c a g g g c g t a c t c a a t g t g t a g a a a c c g a g g t a g t t g c c a c a c c t g a a a c t g t a c c a c c c a g t c c c g

960 40 45 50 55 TGNPNDGYEELNLKSTKGDL mNeonGreen TGNPNDGYEELNLKSTKGDL *VQLGGLTVE a c c g g c a a t c c a a a t g a t g g t t a t g a g g a g t t a a a c c t g a a g t c c a c c a a g g g t g a c c t c t g g c c g t t a g g t t t a c t a c c a a t a c t c c t c a a t t t g g a c t t c a g g t g g t t c c c a c t g g a g

1020 60 65 70 75 QFSPWILVPHIGYGFHQYLP mNeonGreen QFSPWILVPHIGYGFHQYLP LEGGPNQDRMDPIAEMLVQG c a g t t c t c c c c c t g g a t t c t g g t c c c t c a t a t c g g g t a t g g c t t c c a t c a g t a c c t g c c c g t c a a g a g g g g g a c c t a a g a c c a g g g a g t a t a g c c c a t a c c g a a g g t a g t c a t g g a c g g g

1080 80 85 90 95 YPDGMSPFQAAMVDGSGYQV mNeonGreen YPDGMSPFQAAMVDGSGYQV VRVPHRRELGRHYIAGSVLD t a c c c t g a c g g g a t g t c g c c t t t c c a g g c c g c g a t g g t a g a t g g c t c c g g a t a c c a a g t c a t g g g a c t g c c c t a c a g c g g a a a g g t c c g g c g c t a c c a t c t a c c g a g g c c t a t g g t t c a g

1140 100 105 110 115 HRTMQFEDGASLTVNYRYTY mNeonGreen HRTMQFEDGASLTVNYRYTY MACHLKFITGGKSNVVAVGV c a t c g c a c a a t g c a g t t t g a a g a t g g t g c c t c c c t t a c t g t t a a c t a c c g c t a c a c c t a c g t a g c g t g t t a c g t c a a a c t t c t a c c a c g g a g g g a a t g a c a a t t g a t g g c g a t g t g g a t g

1200 120 125 130 135 EGSHIKGEAQVKGTGFPADG mNeonGreen EGSHIKGEAQVKGTGFPADG LSAVDFSLGLHLPSTERSVT g a g g g a a g c c a c a t c a a a g g a g a g g c c c a g g t g a a g g g g a c t g g t t t c c c t g c t g a c g g t c t c c c t t c g g t g t a g t t t c c t c t c c g g g t c c a c t t c c c c t g a c c a a a g g g a c g a c t g c c a

1260 140 145 150 155 PVMTNSLTAADWCRSKKTYP mNeonGreen PVMTNSLTAADWCRSKKTYP RHHGVRQGSRVPAPRLLSVG c c t g t g a t g a c c a a c t c g c t g a c c g c t g c g g a c t g g t g c a g g t c g a a g a a g a c t t a c c c c g g a c a c t a c t g g t t g a g c g a c t g g c g a c g c c t g a c c a c g t c c a g c t t c t t c t g a a t g g g g

AfeI 1320 160 165 170 175 NDKTIISTFKWSYTTGNGKR mNeonGreen NDKTIISTFKWSYTTGNGKR VVFGDDTGKLPTVGSSIALA a a c g a c a a a a c c a t c a t c a g t a c c t t t a a g t g g a g t t a c a c c a c t g g a a a t g g c a a g c g c t t g c t g t t t t g g t a g t a g t c a t g g a a a t t c a c c t c a a t g t g g t g a c c t t t a c c g t t c g c g

1380 180 185 190 195 YRSTARTTYTFAKPMAANYL mNeonGreen YRSTARTTYTFAKPMAANYL VPASRPGGVGKGLWHRSVIQ t a c c g g a g c a c t g c g c g g a c c a c c t a c a c c t t t g c c a a g c c a a t g g c g g c t a a c t a t c t g a t g g c c t c g t g a c g c g c c t g g t g g a t g t g g a a a c g g t t c g g t t a c c g c c g a t t g a t a g a c

1440 200 205 210 215 KNQPMYVFRKTELKHSKTEL mNeonGreen KNQPMYVFRKTELKHSKTEL LVLRHVHETLRLELVGLGLE a a g a a c c a g c c g a t g t a c g t g t t c c g t a a g a c g g a g c t c a a g c a c t c c a a g a c c g a g c t c t t c t t g g t c g g c t a c a t g c a c a a g g c a t t c t g c c t c g a g t t c g t g a g g t t c t g g c t c g a g

1500 220 225 230 235 NFKEWQKAFTDVMGMDELYK mNeonGreen NFKEWQKAFTDVMGMDELYK VELLPLLGKGIHHAHVLQVL a a c t t c a a g g a g t g g c a a a a g g c c t t t a c c g a t g t g a t g g g c a t g g a c g a g c t g t a c a a g t t g a a g t t c c t c a c c g t t t t c c g g a a a t g g c t a c a c t a c c c g t a c c t g c t c g a c a t g t t c

EcoRI 1560 EF 1 5 10 15 MVSKGEEDNMAIIKEFMR mCherry EFMVSKGEEDNMAIIKEFMR FEHHALALLIVHSDDLLEHA g a a t t c a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c t a t c a t c a a g g a g t t c a t g c g c c t t a a g t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g a t a g t a g t t c c t c a a g t a c g c g

1620 20 25 30 35 FKVHMEGSVNGHEFEIEGEG mCherry FKVHMEGSVNGHEFEIEGEG ELHVHLAGHVAVLELDLALA t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g

1680 40 45 50 55 EGRPYEGTQTAKLKVTKGGP mCherry G G T T C G A C T T C C A C T G G T T mCherry-R EGRPYEGTQTAKLKVTKGGP LAAGVLAGLGGLQLHGLTAG g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g

1740 60 65 70 75 LPFAWDILSPQFMYGSKAYV mCherry LPFAWDILSPQFMYGSKAYV QGEGPVDQGRLEHVAGLGVH c t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c

1800 80 85 90 95 KHPADIPDYLKLSFPEGFKW mCherry KHPADIPDYLKLSFPEGFKW LVGGVDGVVQLQGEGLAELP a a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g t t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c

1860 100 105 110 115 ERVMNFEDGGVVTVTQDSSL mCherry ERVMNFEDGGVVTVTQDSSL LAHHVELVAAHHGHGLVGGQ g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c

SbfIPstI 1920 120 125 130 135 QDGEFIYKVKLRGTNFPSDG mCherry C mCherry-F QDGEFIYKVKLRGTNFPSDG LVALEDVLHLQAAGVEGGVA c a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c g t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g

1980 140 145 150 155 PVMQKKTMGWEASSERMYPE mCherry C C C G T A A T G C A G A A G A A G A mCherry-F PVMQKKTMGWEASSERMYPE GYHLLLSHAPLGGGLPHVGL c c c g t a a t g c a g a a g a a g a c t a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g g g c a t t a c g t c t t c t t c t g a t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c

2040 160 165 170 175 DGALKGEIKQRLKLKDGGHY mCherry DGALKGEIKQRLKLKDGGHY VAGQLALDLLPQLQLVAAVV g a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c c t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g

BbvCI 2100 180 185 190 195 DAEVKTTYKAKKPVQLPGAY mCherry DAEVKTTYKAKKPVQLPGAY VSLDLGGVLGLLGHLQGAGV g a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c c t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g

AleI 2160 200 205 210 215 NVNIKLDITSHNEDYTIVEQ mCherry NVNIKLDITSHNEDYTIVEQ VDVDLQVDGGVVLVVGDHFL a a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c

HindIII 2220 220 225 230 235 YERAEGRHSTGGMDELYK* mCherry YERAEGRHSTGGMDELYK* VFAGLAAVGGAAHVLQVLLS t a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t a a g c t a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a t t c g a

2280 G G A C T A T G T C T A A T T T A G pBAD Reverse PQKPPHSFIKLRICILDSRL t g g c t g t t t t g g c g g a t g a g a g a a g a t t t t c a g c c t g a t a c a g a t t a a a t c a g a a c g c a g a c c g a c a a a a c c g c c t a c t c t c t t c t a a a a g t c g g a c t a t g t c t a a t t t a g t c t t g c g t c

2340 LPRIFCFKGPPLLATTGGSG a a g c g g t c t g a t a a a a c a g a a t t t g c c t g g c g g c a g t a g c g c g g t g g t c c c a c c t g a c c c t t c g c c a g a c t a t t t t g t c t t a a a c g g a c c g c c g t c a t c g c g c c a c c a g g g t g g a c t g g g

2400 M c a t g c c g a a c t c a g a a g t g a a a c g c c g t a g c g c c g a t g g t a g t g t g g g g t c t c c c c a t g c g t a c g g c t t g a g t c t t c a c t t t g c g g c a t c g c g g c t a c c a t c a c a c c c c a g a g g g g t a c g

2460 rrnB T1 terminator g a g a g t a g g g a a c t g c c a g g c a t c a a a t a a a a c g a a a g g c t c a g t c g a a a g a c t g g g c c t c t c t c a t c c c t t g a c g g t c c g t a g t t t a t t t t g c t t t c c g a g t c a g c t t t c t g a c c c g g a

2520 rrnB T1 terminator t t c g t t t t a t c t g t t g t t t g t c g g t g a a c g c t c t c c t g a g t a g g a c a a a t c c g c c g g g a g a a g c a a a a t a g a c a a c a a a c a g c c a c t t g c g a g a g g a c t c a t c c t g t t t a g g c g g c c c t c

2580 c g g a t t t g a a c g t t g c g a a g c a a c g g c c c g g a g g g t g g c g g g c a g g a c g c c c g c c a t a a a g c c t a a a c t t g c a a c g c t t c g t t g c c g g g c c t c c c a c c g c c c g t c c t g c g g g c g g t a t t t

2640 rrnB T2 terminator c t g c c a g g c a t c a a a t t a a g c a g a a g g c c a t c c t g a c g g a t g g c c t t t t t g c g t t t c t a c g a c g g t c c g t a g t t t a a t t c g t c t t c c g g t a g g a c t g c c t a c c g g a a a a a c g c a a a g a t g

2700 AmpR promoter a a a c t c t t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a t t t g a g a a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t

2760 1 5 signal sequence MSIQHFR AmpR promoter AmpR MSIQHFR c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a

2820 10 15 20 signal sequence VALIPFFAAFCLPVFA 25 HPET AmpR VALIPFFAAFCLPVFAHPET g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c

2880 30 35 40 45 LVKVKDAEDQLGARVGYIEL AmpR LVKVKDAEDQLGARVGYIEL c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c

2940 50 55 60 65 DLNSGKILESFRPEERFPMM AmpR DLNSGKILESFRPEERFPMM g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c

3000 70 75 80 85 STFKVLLCGAVLSRVDAGQE AmpR C G A T A C A C C G C G C C A T A A T A Amp-R STFKVLLCGAVLSRVDAGQE a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c

ScaI 3060 90 95 100 105 QLGRRIHYSQNDLVEYSPVT AmpR QLGRRIHYSQNDLVEYSPVT c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t

3120 110 115 120 125 EKHLTDGMTVRELCSAAITM AmpR EKHLTDGMTVRELCSAAITM g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c

PvuI 3180 130 135 140 145 SDNTAANLLLTTIGGPKELT AmpR SDNTAANLLLTTIGGPKELT a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g

3240 150 155 160 165 AFLHNMGDHVTRLDRWEPEL AmpR AFLHNMGDHVTRLDRWEPEL g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c

3300 170 175 180 185 NEAIPNDERDTTMPVAMATT AmpR NEAIPNDERDTTMPVAMATT a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c

FspI 3360 190 195 200 205 LRKLLTGELLTLASRQQLID AmpR LRKLLTGELLTLASRQQLID t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g

3420 210 215 220 225 WMEADKVAGPLLRSALPAGW AmpR WMEADKVAGPLLRSALPAGW t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c

3480 230 235 240 245 FIADKSGAGERGSRGIIAAL AmpR FIADKSGAGERGSRGIIAAL t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c

3540 250 255 260 265 GPDGKPSRIVVIYTTGSQAT AmpR GPDGKPSRIVVIYTTGSQAT g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a

3600 270 275 280 285 MDERNRQIAEIGASLIKHW* AmpR MDERNRQIAEIGASLIKHW* a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t

3660 c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a

3720 a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c

3780 ori t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a

3840 ori t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a

3900 ori t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c

3960 ori c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a

4020 ori g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g

4080 ori g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c

4140 ori t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t

4200 ori c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c

4260 ori G pBR322ori-F g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c

4320 ori G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t

4380 ori t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a

PciI 4440 t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a

4500 g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t

BspQISapI 4560 bom A G C G A G T C A G T G A G C G A G L4440 a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a

NdeI 4620 bom A T G T T A G A C G pRS-marker c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g

Tth111IPflFIAccIBstZ17I 4680 bom A G A C T A C G G C pRS-marker t c t g a t g c c g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g a g a c t a c g g c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c

4740 bom c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c

PfoI 4800 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g

MauBI 4860 c g t c a t c a c c g a a a c g c g c g a g g c a g c a g a t c a a t t c g c g c g c g a a g g c g a a g c g g c a t g g c a g t a g t g g c t t t g c g c g c t c c g t c g t c t a g t t a a g c g c g c g c t t c c g c t t c g c c g t a c

NsiI 4920 lacIq promoter c a t t t a c g t t g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g t a a a t g c a a c t g t g g t a g c t t a c c a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g

4980 1 5 10 MKPVTLYDVAE lacIq promoter lacI T G C T A C A G C G T C T LacI-R g g a a g a g a g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a c c t t c t c t c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t

5040 15 20 25 30 YAGVSYQTVSRVVNQASHVS lacI C A T A C G G LacI-R g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g

5100 35 40 45 50 AKTREKVEAAMAELNYIPNR lacI MAELNYIPNR t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c

5160 55 60 65 70 VAQQLAGKQSLLIGVATSSL lacI VAQQLAGKQSLLIGVATSSL c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a

5220 75 80 85 90 ALHAPSQIVAAIKSRADQLG lacI ALHAPSQIVAAIKSRADQLG g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c

5280 95 100 105 110 ASVVVSMVERSGVEACKAAV lacI ASVVVSMVERSGVEACKAAV t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a

BclI*MluI 5340 115 120 125 130 HNLLAQRVSGLIINYPLDDQ lacI HNLLAQRVSGLIINYPLDDQ g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t

5400 135 140 145 150 DAIAVEAACTNVPALFLDVS lacI DAIAVEAACTNVPALFLDVS g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g

5460 155 160 165 170 DQTPINSIIFSHEDGTRLGV lacI DQTPINSIIFSHEDGTRLGV t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a

PspOMIApaI 5520 175 180 185 190 EHLVALGHQQIALLAGPLSS lacI EHLVALGHQQIALLAGPLSS g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g

5580 195 200 205 210 VSARLRLAGWHKYLTRNQIQ lacI VSARLRLAGWHKYLTRNQIQ t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t

5640 215 220 225 230 PIAEREGDWSAMSGFQQTMQ lacI PIAEREGDWSAMSGFQQTMQ g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t

5700 235 240 245 250 MLNEGIVPTAMLVANDQMAL lacI MLNEGIVPTAMLVANDQMAL a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a

EcoRV 5760 255 260 265 270 GAMRAITESGLRVGADISVV lacI GAMRAITESGLRVGADISVV g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g t c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c a

5820 275 280 285 290 GYDDTEDSSCYIPPLTTIKQ lacI GYDDTEDSSCYIPPLTTIKQ g g g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a c c c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t

5880 295 300 305 310 DFRLLGQTSVDRLLQLSQGQ lacI DFRLLGQTSVDRLLQLSQGQ g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t

5940 315 320 325 330 AVKGNQLLPVSLVKRKTTLA lacI AVKGNQLLPVSLVKRKTTLA g g c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c c c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g

6000 335 340 345 350 PNTQTASPRALADSLMQLAR lacI PNTQTASPRALADSLMQLAR g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c

6060 355 360 QVSRLESGQ* lacI QVSRLESGQ* a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c g c g t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g c g c

6120 a a t t g a t c t g g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a t t a a c t a g a c c a a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t

6180 g g c a g c c a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c c g t c g g t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a

3ʹ 5ʹ 6210 c g c t c a a g g c g c a c t c c c g t t c t g g a t a a t g c g a g t t c c g c g t g a g g g c a a g a c c t a t t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.