Addgene OVA-miSFIT-17-T Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: OVA-miSFIT-17-T Sequencing Result


Map View

PciI (10,326) L4440 (10,216 .. 10,233) CAP binding site lac promoter M13/pUC Reverse (9998 .. 10,020) lac operator M13 Reverse (9985 .. 10,001) M13 rev T3 (9946 .. 9966) T3 promoter NdeI (9593) SnaBI (9489) 5' LTR (truncated) NruI * (8867) NotI (8552) gp41 peptide KflI - PpuMI (7767) BspDI * - ClaI * (7551) BclI * (7521) BsaBI * (7520) SV40 poly(A) signal AgeI (7215) BmtI (7190) NheI (7186) EGFP-C (7094 .. 7115) EXFP-R (6747 .. 6766) EGFP-N (6486 .. 6507) SbfI (5348) minimal CMV promoter pBR322ori-F (81 .. 100) Amp-R (1383 .. 1402) F1ori-F (1888 .. 1909) F1ori-R (2100 .. 2119) M13/pUC Forward (2333 .. 2355) M13 Forward (2347 .. 2364) M13 fwd T7 (2374 .. 2393) T7 promoter AvrII (2409) SfiI (2463) Acc65I (3080) KpnI (3084) WPRE-R (3616 .. 3636) SalI (3690) Bsu36I (3904) SphI (4460) AfeI (4571) RsrII (4588) TspMI - XmaI (4679) SmaI (4681) BspEI (4748) hPGK-F (4756 .. 4774) AscI (4853) BlpI (4863) XcmI (5175) OVA-miSFIT-17-T 10,386 bp

Sequence View

60 ori t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c

120 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c

180 ori c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c

240 ori c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g

300 ori c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t

360 ori c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c

420 ori t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g

480 ori t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g

540 ori c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c

600 ori t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a

660 g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a

720 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t

780 285 *WHKILS AmpR *WHKILS a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t

840 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a

900 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c

960 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t

1020 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t

1080 200 195 190 185 ALTLLEGTLLKRLTTAMAVP AmpR ALTLLEGTLLKRLTTAMAVP a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c

1140 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g

1200 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g

1260 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t

1320 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g

1380 100 95 90 85 LDNQSYHIRRGLQEQGADIR AmpR LDNQSYHIRRGLQEQGADIR c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c

1440 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g

1500 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c

1560 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g

1620 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR PLCFAAFFPILAVRFHQISM a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a

1680 AmpR promoter a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t

1740 AmpR promoter c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t

1800 f1 ori a g t g c c a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a t c a c g g t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t

1860 f1 ori a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t

1920 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g

1980 f1 ori g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t

2040 f1 ori c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a

2100 f1 ori A F1ori-R a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t

2160 f1 ori G G G A A G A A A G C G A A A G G A G F1ori-R g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g

2220 f1 ori g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g

2280 a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c t g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g a c

2340 C C C A G T C A M13/pUC Forward g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t c a c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a g t

2400 M13 fwd T7 promoter C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C A C T A T A G G G T7 c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g a c t c a c t a t a g g g c g a a t t g g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c t g a g t g a t a t c c c g c t t a a c

AvrII 2460 SV40 ori g g t a c g t c c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c t c a g a g g c c g c c a t g c a g g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c g a g t c t c c g g c

SfiI 2520 SV40 ori a g g c g g c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g t c c g c c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c

2580 SV40 ori c g g a a c t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a t a g c t a g a g c c a g c c t t g a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t a t c g a t c t c g g t

2640 SV40 poly(A) signal g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t

2700 SV40 poly(A) signal t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a

2760 SV40 poly(A) signal a a a c a a g t t c c t c t c a c t c t c t g a t a t t c a t t t c t t t g c a a g t t a t a a a t a c t g a a t a a t t t t g t t c a a g g a g a g t g a g a g a c t a t a a g t a a a g a a a c g t t c a a t a t t t a t g a c t t a t t a

2820 3' LTR ( Δ U3) a a g a t g a c a t g a a c t a c t a c t g c t a g a g a t t t t c c a c a c t g a c t a a a a g g g t c t g a g g g a t t c t a c t g t a c t t g a t g a t g a c g a t c t c t a a a a g g t g t g a c t g a t t t t c c c a g a c t c c c t

2880 3' LTR ( Δ U3) t c t c t a g t t a c c a g a g t c a c a c a a c a g a c g g g c a c a c a c t a c t t g a a g c a c t c a a g g c a a a g a g a t c a a t g g t c t c a g t g t g t t g t c t g c c c g t g t g t g a t g a a c t t c g t g a g t t c c g t t

2940 3' LTR ( Δ U3) g c t t t a t t g a g g c t t a a g c a g t g g g t t c c c t a g t t a g c c a g a g a g c t c c c a g g c t c a g a t c g a a a t a a c t c c g a a t t c g t c a c c c a a g g g a t c a a t c g g t c t c t c g a g g g t c c g a g t c t a

3000 3' LTR ( Δ U3) c t g g t c t a a c c a g a g a g a c c c a g t a c a a g c a a a a a g c a g a t c t t g t c t t c g t t g g g a g t g g a c c a g a t t g g t c t c t c t g g g t c a t g t t c g t t t t t c g t c t a g a a c a g a a g c a a c c c t c a c

3060 3' LTR ( Δ U3) a a t t a g c c c t t c c a g t c c c c c c t t t t c t t t t a a a a a g t g g c t a a g a t c t a c a g c t g c c t t t t a a t c g g g a a g g t c a g g g g g g a a a a g a a a a t t t t t c a c c g a t t c t a g a t g t c g a c g g a a

Acc65IKpnI 3120 WPRE *LYRARIGPPSAAWL g t a a g t c a t t g g t c t t a a a g g t a c c g a g c t c g a a t t c c a g g c g g g g a g g c g g c c c a a a g g c a t t c a g t a a c c a g a a t t t c c a t g g c t c g a g c t t a a g g t c c g c c c c t c c g c c g g g t t t c c

3180 WPRE SIRSTQPRLRLRPLPRLAPL g a g a t c c g a c t c g t c t g a g g g c g a a g g c g a a g a c g c g g a a g a g g c c g c a g a g c c g g c a g c c t c t a g g c t g a g c a g a c t c c c g c t t c c g c t t c t g c g c c t t c t c c g g c g t c t c g g c c g t c g

3240 WPRE 1 IEGR (in frame with Factor Xa site) RDVAEGR Factor Xa site LGRSPLDAPNLASPVYCFST a g g c c g c g g g a a g g a a g g t c c g c t g g a t t g a g g g c c g a a g g g a c g t a g c a g a a g g a c g t c t c c g g c g c c c t t c c t t c c a g g c g a c c t a a c t c c c g g c t t c c c t g c a t c g t c t t c c t g c a g

3300 WPRE (in frame with Factor Xa site) PAQNPGGNTGEQPWKGRQLP GRLIWTAVCALLWPFSTLKG c c g c g c a g a a t c c a g g t g g c a a c a c a g g c g a g c a g c c a t g g a a a g g a c g t c a g c t t c c c c g g c g c g t c t t a g g t c c a c c g t t g t g t c c g c t c g t c g g t a c c t t t c c t g c a g t c g a a g g g g

3360 WPRE (in frame with Factor Xa site) RQHHGIVSAQQPSPCPAAGK SLVVSNDTGLLRAGTWCRAL g a c a a c a c c a c g g a a t t g t c a g t g c c c a a c a g c c g a g c c c c t g t c c a g c a g c g g g c a a g g c t g t t g t g g t g c c t t a a c a g t c a c g g g t t g t c g g c t c g g g g a c a g g t c g t c g c c c g t t c c

3420 WPRE (in frame with Factor Xa site) AGGDEFRRGNREGESESPGK CAAILEATAIPLPFAFTGSL c a g g c g g c g a t g a g t t c c g c c g t g g c a a t a g g g a g g g g g a a a g c g a a a g t c c c g g a a a g g g t c c g c c g c t a c t c a a g g c g g c a c c g t t a t c c c t c c c c c t t t c g c t t t c a g g g c c t t t c c

3480 WPRE (in frame with Factor Xa site) ELTGGGNAPTSGGCVSKHSA LQCTTAIGWGTPTADAFVTC a g c t g a c a g g t g g t g g c a a t g c c c c a a c c a g t g g g g g t t g c g t c a g c a a a c a c a g t g c a c t c g a c t g t c c a c c a c c g t t a c g g g g t t g g t c a c c c c c a a c g c a g t c g t t t g t g t c a c g t g

3540 WPRE (in frame with Factor Xa site) HHATLPDNGPQLLIKRQQPG VVGRQRVVPWLEEYLSLLWS a c c a c g c c a c g t t g c c t g a c a a c g g g c c a c a a c t c c t c a t a a a g a g a c a g c a a c c a g g a t t g g t g c g g t g c a a c g g a c t g t t g c c c g g t g t t g a g g a g t a t t t c t c t g t c g t t g g t c c t a

3600 WPRE (in frame with Factor Xa site) FIQGGENESHTGSNSMIQRH KYLSSFIFAMRSAIAHYLPM t t a t a c a a g g a g g a g a a a a t g a a a g c c a t a c g g g a a g c a a t a g c a t g a t a c a a a g g c a t t a a t a t g t t c c t c c t c t t t t a c t t t c g g t a t g c c c t t c g t t a t c g t a c t a t g t t t c c g t a a

3660 WPRE * C A T A G C G T A A A A G G A G C A A C A WPRE-R a a a g c a g c g t a t c c a c a t a g c g t a a a a g g a g c a a c a t a g t t a a g a a t a c c a g t c a a t c t t t t t c g t c g c a t a g g t g t a t c g c a t t t t c c t c g t t g t a t c a a t t c t t a t g g t c a g t t a g a a

SalI 3720 WPRE *LIG t c a c a a a t t t t g t a a t c c a g a g g t t g a t t g t c g a c t c t a g a c t c g a c t c t a g a g g a t c c c a g t g t t t a a a a c a t t a g g t c t c c a a c t a a c a g c t g a g a t c t g a g c t g a g a t c t c c t a g g g

3780 RNWRKFAIYAVLGVVVAALI c c t g t t c c a c c t c t t g a a g g c t a t g t a g g c c a c a a g g c c c a c a a c c a c a g c a g c c a g g a t g g a c a a g g t g g a g a a c t t c c g a t a c a t c c g g t g t t c c g g g t g t t g g t g t c g t c g g t c c t a

3840 SCYVPILNDTTGRTVVPQSS g g a g c a a t a g a c a g g g a t g a g g t t g t c g g t g g t g c c t c g g g t c a c c a c g g g c t g g g a g c t c c t c g t t a t c t g t c c c t a c t c c a a c a g c c a c c a c g g a g c c c a g t g g t g c c c g a c c c t c g a

3900 GMVTTVVGAVTSAILDQEPP g c c c a t c a c t g t g g t c a c c a c a c c t g c c a c c g t g c t g g c t a t g a g g t c t t g t t c t g g a g g c g g g t a g t g a c a c c a g t g g t g t g g a c g g t g g c a c g a c c g a t a c t c c a g a a c a a g a c c t c c

Bsu36I 3960 AEPEQTSPATSDSGEPPTSR t g c c t c a g g c t c c t g g g t g c t g g g g g c t g t g c t g t c c g a g c c c t c t g g g g g t g t g g a c c g a c g g a g t c c g a g g a c c c a c g a c c c c c g a c a c g a c a g g c t c g g g a g a c c c c c a c a c c t g g c

4020 TIWRGPIEECEADAWRTCER t g t a a t c c a a c g g c c a g g g a t c t c c t c g c a c t c g g c g t c g g c c c a g c g t g t g c a c t c g c g a c a t t a g g t t g c c g g t c c c t a g a g g a g c g t g a g c c g c a g c c g g g t c g c a c a c g t g a g c g c

4080 LQRETDECVTCPLCPDVHNA g a g c t g g c g c t c g g t g t c c t c g c a c a c g g t g c a g g g c a g g c a c g g g t c c a c g t g g t t g g c c t c g a c c g c g a g c c a c a g g a g c g t g t g c c a c g t c c c g t c c g t g c c c a g g t g c a c c a a c c g

4140 EDSYTGDPCEECVTNQKDQC c t c g t c g g a a t a c g t g c c g t c g g g g c a c t c c t c g c a c a c g g t g t t c t g c t t g t c c t g g c a g a g c a g c c t t a t g c a c g g c a g c c c c g t g a g g a g c g t g t g c c a c a a g a c g a a c a g g a c c g t

4200 SFVLGSGAECVRCAECRGTT g g a g a a c a c g a g g c c c g a g c c c g c c t c g c a c a c g c g g c a c g c c t c g c a g c g c c c a g t c g t c c t c t t g t g c t c c g g g c t c g g g c g g a g c g t g t g c g c c g t g c g g a g c g t c g c g g g t c a g c a

4260 EDQYYGYACRCVADDAEVCP c t c a t c c t g g t a g t a g c c g t a g g c g c a g c g g c a c a c g g c g t c g t c g g c c t c c a c g c a c g g g a g t a g g a c c a t c a t c g g c a t c c g c g t c g c c g t g t g c c g c a g c a g c c g g a g g t g c g t g c c

4320 ASMSQLGVCETCPKCPETAS c g c c g a c a t g c t c t g g a g c c c c a c g c a c t c g g t g c a c g g c t t g c a c g g c t c g g t c g c g c t g c g g c t g t a c g a g a c c t c g g g g t g c g t g a g c c a c g t g c c g a a c g t g c c g a g c c a g c g c g a

4380 VVDSFTVSDLCPECVTQNAG c a c c a c g t c g g a g a a c g t c a c g c t g t c c a g g c a g g g c t c a c a c a c g g t c t g g t t g g c t c c g t g g t g c a g c c t c t t g c a g t g c g a c a g g t c c g t c c c g a g t g t g t g c c a g a c c a a c c g a g g

4440 CPQAVGEGLNCAKCCEGSHT a c a a g g c t g g g c c a c a c c c t c g c c c a g g t t g c a g g c t t t g c a g c a c t c a c c g c t g t g t g t t g t t c c g a c c c g g t g t g g g a g c g g g t c c a a c g t c c g a a a c g t c g t g a g t g g c g a c a c a c a

SphI 4500 YLGTPCAEKAGGLSVGLLLL g t a c a g g c c t g t g g g g c a t g c c t c c t t g g c a c c t c c a a g g g a c a c c c c c a g a a g c a g c a a c a t g t c c g g a c a c c c c g t a c g g a g g a a c c g t g g a g g t t c c c t g t g g g g g t c t t c g t c g t t

4560 LLLRPGDMARGTAGAGMARR c a g c a g c a g g c g c g g c c c g t c c a t g g c g c g g c c g g t g g c a c c t g c c c c c a t c g c c c g c c t g t c g t c g t c c g c g c c g g g c a g g t a c c g c g c c g g c c a c c g t g g a c g g g g g t a g c g g g c g g a

RsrIIAfeI 4620 GRCREVELETRKASQHPQAA c c c g c g g c a g c g c t c g a c t t c c a g c t c g g t c c g c t t t g c g g a c t g a t g g g g c t g c g c t g c g g g c g c c g t c g c g a g c t g a a g g t c g a g c c a g g c g a a a c g c c t g a c t a c c c c g a c g c g a c g

TspMIXmaI 4680 SRELAGGARRLQGRGSNRCG g c t g c g c t c c a g c g c c c c c c c t g c c c g c c g g a g c t g g c c g c g g c c c g a a t t c c t g c a g c c c g a c g c g a g g t c g c g g g g g g g a c g g g c g g c c t c g a c c g g c g c c g g g c t t a a g g a c g t c g g

SmaI 4740 hPGK promoter PPDGQPSLDTIRDVLSGVAS c g g g g g a t c c c c c t g g g g a g a g a g g t c g g t g a t t c g g t c a a c g a g g g a g c c g a c t g c c g a g c c c c c t a g g g g g a c c c c t c t c t c c a g c c a c t a a g c c a g t t g c t c c c t c g g c t g a c g g c t

BspEI 4800 hPGK promoter G A A C G T C T T A C G C C T T G T G hPGK-F TREPPKCFASCRAPLFLAWV c g t g c g c t c c g g a g g c t t g c a g a a t g c g g a a c a c c g c g c g g g c a g g a a c a g g g c c c a c a c g c a c g c g a g g c c t c c g a a c g t c t t a c g c c t t g t g g c g c g c c c g t c c t t g t c c c g g g t g t g

AscI 4860 hPGK promoter VAGCGAERVAGERGSSEARG t a c c g c c c c a c a c c c c g c c t c c c g c a c c g c c c c t t c c c g g c c g c t g c t c t c g g c g c g c c c a t g g c g g g g t g t g g g g c g g a g g g c g t g g c g g g g a a g g g c c g g c g a c g a g a g c c g c g c g g g

BlpI 4920 hPGK promoter ASCGSNAVAWRPRRAAM t g c t g a g c a g c c g c t a t t g g c c a c a g c c c a t c g c g g t c g g c g c g c t g c c a t t g c t c c c t g a c g a c t c g t c g g c g a t a a c c g g t g t c g g g t a g c g c c a g c c g c g c g a c g g t a a c g a g g g a c

4980 hPGK promoter g c g c t g t c c g t c t g c g a g g g t a c t a g t g a g a c g t g c g g c t t c c g t t t g t c a c g t c c g g c a c g c g a c a g g c a g a c g c t c c c a t g a t c a c t c t g c a c g c c g a a g g c a a a c a g t g c a g g c c g t

5040 hPGK promoter c g c c g c g a a c c g c a a g g a a c c t t c c c g a c t t a g g g g c g g a g c a g g a a g c g t c g c c g g g g g g c g g c g c t t g g c g t t c c t t g g a a g g g c t g a a t c c c c g c c t c g t c c t t c g c a g c g g c c c c c

5100 hPGK promoter g c c c a c a a g g g t a g c g g c g a a g a t c c g g g t g a c g c t g c g a a c g g a c g t g a a g a a t g t g c g c g g g t g t t c c c a t c g c c g c t t c t a g g c c c a c t g c g a c g c t t g c c t g c a c t t c t t a c a c g c

5160 hPGK promoter a g a c c c a g g g t c g g c g c c g c t g c g t t t c c c g g a a c c a c g c c c a g a g c a g c c g c g t c c c t g t c t g g g t c c c a g c c g c g g c g a c g c a a a g g g c c t t g g t g c g g g t c t c g t c g g c g c a g g g a c

XcmI 5220 hPGK promoter c g c a a a c c c a g g g c t g c c t t g g a a a a g g c g c a a c c c c a a c c c c g t g g a a t t c g a t a t c a a g c g t t t g g g t c c c g a c g g a a c c t t t t c c g c g t t g g g g t t g g g g c a c c t t a a g c t a t a g t t

5280 minimal CMV promoter g c t t c t c g a g g g t a g g c g t g t a c g g t g g g a g g c c t a t a t a a g c a g a g c t c g t t t a g t g a a c g a a g a g c t c c c a t c c g c a c a t g c c a c c c t c c g g a t a t a t t c g t c t c g a g c a a a t c a c t t

5340 c c g t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g a g g g c a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c t c

SbfI 5400 MSTRTQINKVVRFD c t c c t g c a g g t g c c g c c a t g a g c a c c a g g a c a c a a a t a a a t a a g g t t g t t c g c t t t g a t a g a g g a c g t c c a c g g c g g t a c t c g t g g t c c t g t g t t t a t t t a t t c c a a c a a g c g a a a c t a t

5460 KLPGFGDSIEAQCGTSVNVH a a c t t c c a g g a t t c g g a g a c a g t a t t g a a g c t c a g t g t g g c a c a t c t g t a a a c g t t c a c t t t g a a g g t c c t a a g c c t c t g t c a t a a c t t c g a g t c a c a c c g t g t a g a c a t t t g c a a g t g a

5520 SSLRDILNQITKPNDVYSFS c t t c a c t t a g a g a c a t c c t c a a c c a a a t c a c c a a a c c a a a t g a t g t t t a t t c g t t c a g c c g a a g t g a a t c t c t g t a g g a g t t g g t t t a g t g g t t t g g t t t a c t a c a a a t a a g c a a g t c g g

5580 LASRLYAEERYPILPEYLQC t t g c c a g t a g a c t t t a t g c t g a a g a g a g a t a c c c a a t c c t g c c a g a a t a c t t g c a g t g t g a a c g g t c a t c t g a a a t a c g a c t t c t c t c t a t g g g t t a g g a c g g t c t t a t g a a c g t c a c a c

5640 VKELYRGGLEPINFQTAADQ t g a a g g a a c t g t a t a g a g g a g g c t t g g a a c c t a t c a a c t t t c a a a c a g c t g c a g a t c a a g a c t t c c t t g a c a t a t c t c c t c c g a a c c t t g g a t a g t t g a a a g t t t g t c g a c g t c t a g t t c

5700 ARELINSWVESQTNGIIRNV c c a g a g a g c t c a t c a a t t c c t g g g t a g a a a g t c a g a c a a a t g g a a t t a t c a g a a a t g t c c g g t c t c t c g a g t a g t t a a g g a c c c a t c t t t c a g t c t g t t t a c c t t a a t a g t c t t t a c a g g

5760 LQPSSVDSQTAMVLVNAIVF t t c a g c c a a g c t c c g t g g a t t c t c a a a c t g c a a t g g t t c t g g t t a a t g c c a t t g t c t t c a a a g t c g g t t c g a g g c a c c t a a g a g t t t g a c g t t a c c a a g a c c a a t t a c g g t a a c a g a a g t

5820 KGLWEKAFKDEDTQAMPFRV a a g g a c t g t g g g a g a a a g c a t t t a a g g a t g a a g a c a c a c a a g c a a t g c c t t t c a g a g t g a t t c c t g a c a c c c t c t t t c g t a a a t t c c t a c t t c t g t g t g t t c g t t a c g g a a a g t c t c a c t

5880 TEQESKPVQMMYQIGLFRVA c t g a g c a a g a a a g c a a a c c t g t g c a g a t g a t g t a c c a g a t t g g t t t a t t t a g a g t g g c a t g a c t c g t t c t t t c g t t t g g a c a c g t c t a c t a c a t g g t c t a a c c a a a t a a a t c t c a c c g t a

5940 SMASEKMKILELPFASGTMS c a a t g g c t t c t g a g a a a a t g a a g a t c c t g g a g c t t c c a t t t g c c a g t g g g a c a a t g a g c a g t t a c c g a a g a c t c t t t t a c t t c t a g g a c c t c g a a g g t a a a c g g t c a c c c t g t t a c t c g t

6000 1 SIIN OVA peptide MLVLLPDEVSGLEQLESIIN t g t t g g t g c t g t t g c c t g a t g a a g t c t c a g g c c t t g a g c a g c t t g a g a g t a t a a t c a a c t a c a a c c a c g a c a a c g g a c t a c t t c a g a g t c c g g a a c t c g t c g a a c t c t c a t a t t a g t t g a

6060 5 FEKL (in frame with OVA peptide) TEWTSSNVMEERKIKV OVA peptide FEKLTEWTSSNVMEERKIKV t t g a a a a a c t g a c t g a a t g g a c c a g t t c t a a t g t t a t g g a a g a g a g g a a g a t c a a a g t g t a a c t t t t t g a c t g a c t t a c c t g g t c a a g a t t a c a a t a c c t t c t c t c c t t c t a g t t t c a c a

6120 (in frame with OVA peptide) YLPRMKMEEKYNLTSVLMAM YLPRMKMEEKYNLTSVLMAM a c t t a c c t c g c a t g a a g a t g g a g g a a a a a t a c a a c c t c a c a t c t g t c t t a a t g g c t a t g g t g a a t g g a g c g t a c t t c t a c c t c c t t t t t a t g t t g g a g t g t a g a c a g a a t t a c c g a t a c c

6180 (in frame with OVA peptide) GITDVFSSSANLSGISSAES GITDVFSSSANLSGISSAES g c a t t a c t g a c g t g t t t a g c t c t t c a g c c a a t c t g t c t g g c a t c t c c t c a g c a g a g a g c c c g t a a t g a c t g c a c a a a t c g a g a a g t c g g t t a g a c a g a c c g t a g a g g a g t c g t c t c t c g g

6240 (in frame with OVA peptide) LKISQAVHAAHAEINEAGRE LKISQAVHAAHAEINEAGRE t g a a g a t a t c t c a a g c t g t c c a t g c a g c a c a t g c a g a a a t c a a t g a a g c a g g c a g a g a g g a c t t c t a t a g a g t t c g a c a g g t a c g t c g t g t a c g t c t t t a g t t a c t t c g t c c g t c t c t c c

6300 (in frame with OVA peptide) VVGSAEAGVDAASVSEEFRA VVGSAEAGVDAASVSEEFRA t g g t a g g g t c a g c a g a g g c t g g a g t g g a t g c t g c a a g c g t c t c t g a a g a a t t t a g g g c t g a c c a t c c c a g t c g t c t c c g a c c t c a c c t a c g a c g t t c g c a g a g a c t t c t t a a a t c c c g a c

6360 (in frame with OVA peptide) DHPFLFCIKHIATNAVLFFG DHPFLFCIKHIATNAVLFFG a c c a t c c a t t c c t c t t c t g t a t c a a g c a c a t c g c a a c c a a c g c c g t t c t c t t c t t t g g c a t g g t a g g t a a g g a g a a g a c a t a g t t c g t g t a g c g t t g g t t g c g g c a a g a g a a g a a a c c g t

6420 (in frame with OVA peptide) RCVSPEFGSG 140 145 EGRGSLLTCG T2A RCVSPEFGSGEGRGSLLTCG g a t g t g t t t c c c c t g a a t t c g g c a g t g g a g a g g g c a g a g g a a g t c t g c t a a c a t g c g g t g c t a c a c a a a g g g g a c t t a a g c c g t c a c c t c t c c c g t c t c c t t c a g a c g a t t g t a c g c c a c

6480 150 155 DVEENPGP 1a V 5 10 SKGEELFTGVV T2A EGFP 1a V 5 10 SKGEELFTGVV EGFP DVEENPGPVSKGEELFTGVV a c g t c g a g g a g a a t c c t g g c c c a g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c t g c a g c t c c t c t t a g g a c c g g g t c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g

6540 15 20 25 30 PILVELDGDVNGHKFSVSGE EGFP 15 20 25 30 PILVELDGDVNGHKFSVSGE EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N PILVELDGDVNGHKFSVSGE c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c

6600 35 40 45 50 GEGDATYGKLTLKFICTTGK EGFP 35 40 45 50 GEGDATYGKLTLKFICTTGK EGFP GEGDATYGKLTLKFICTTGK g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g

6660 55 60 65 70 LPVPWPTLVTTLTYGVQCFS EGFP 55 60 65 70 LPVPWPTLVTTLTYGVQCFS EGFP LPVPWPTLVTTLTYGVQCFS t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g

6720 75 80 85 90 RYPDHMKQHDFFKSAMPEGY EGFP 75 80 85 90 RYPDHMKQHDFFKSAMPEGY EGFP RYPDHMKQHDFFKSAMPEGY g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c

6780 95 100 105 110 VQERTIFFKDDGNYKTRAEV EGFP 95 100 105 110 VQERTIFFKDDGNYKTRAEV EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R VQERTIFFKDDGNYKTRAEV t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t

6840 115 120 125 130 KFEGDTLVNRIELKGIDFKE EGFP 115 120 125 130 KFEGDTLVNRIELKGIDFKE EGFP KFEGDTLVNRIELKGIDFKE a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c

6900 135 140 145 150 DGNILGHKLEYNYNSHNVYI EGFP 135 140 145 150 DGNILGHKLEYNYNSHNVYI EGFP DGNILGHKLEYNYNSHNVYI a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t

6960 155 160 165 170 MADKQKNGIKVNFKIRHNIE EGFP 155 160 165 170 MADKQKNGIKVNFKIRHNIE EGFP MADKQKNGIKVNFKIRHNIE t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c

7020 175 180 185 190 DGSVQLADHYQQNTPIGDGP EGFP 175 180 185 190 DGSVQLADHYQQNTPIGDGP EGFP DGSVQLADHYQQNTPIGDGP a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c

7080 195 200 205 210 VLLPDNHYLSTQSALSKDPN EGFP 195 200 205 210 VLLPDNHYLSTQSALSKDPN EGFP VLLPDNHYLSTQSALSKDPN t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c

7140 215 220 225 230 EKRDHMVLLEFVTAAGITLG EGFP 215 220 225 230 EKRDHMVLLEFVTAAGITLG EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C EKRDHMVLLEFVTAAGITLG a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t

BmtINheI 7200 235 MDELYK EF* EGFP 235 MDELYK EF* EGFP MDELYKEF* t g g a c g a g c t g t a c a a g g a a t t c t a a c t a g a g c t c g c a c g t g a g t g c t a g c c t a c c t g c a a c c t g c t c g a c a t g t t c c t t a a g a t t g a t c t c g a g c g t g c a c t c a c g a t c g g a t g g a c g t

AgeI 7260 c t g t a a g t a c t t t g a c c g g t a c t a g c t t a g t c a t g c a c c g g g c t g c a g g a a t t c g a t g a c g a c a t t c a t g a a a c t g g c c a t g a t c g a a t c a g t a c g t g g c c c g a c g t c c t t a a g c t a c t g

7320 SV40 poly(A) signal a g g c g c a g c t t c c g a g g g a t t t g a g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a t c c g c g t c g a a g g c t c c c t a a a c t c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t

7380 SV40 poly(A) signal c a a a c c a a a a c t a g a a t g c a g t g a a a a a a a t g c c t t a t t t g t g a a a t t t g t g a t g c t a t t g t t t g g t t t t g a t c t t a c g t c a c t t t t t t t a c g g a a t a a a c a c t t t a a a c a c t a c g a t a a

7440 SV40 poly(A) signal g c c t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t c g g a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a

7500 (in frame with gp41 peptide) *TEPEPPPTPPKKFCTFGRC *TEPEPPPTPPKKFCTFGRC t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g

BspDI*ClaI*BsaBI*BclI* 7560 (in frame with gp41 peptide) IHYPQNHDSRELSTEHDIFN cPPT/CTS IHYPQNHDSRELSTEHDIFN a a a t g t g g t a t g g c t g a t t a t g a t c a g a t c t c t c g a g g c t a g t c t c g t g a t c g a t a a a a t t t t a c a c c a t a c c g a c t a a t a c t a g t c t a g a g a g c t c c g a t c a g a g c a c t a g c t a t t t t a

7620 (in frame with gp41 peptide) QIKTIQKQLEKTQIDTAIID cPPT/CTS QIKTIQKQLEKTQIDTAIID t t t g a a t t t t t g t a a t t t g t t t t t g t a a t t c t t t a g t t t g t a t g t c t g t t g c t a t t a t g t a a a c t t a a a a a c a t t a a a c a a a a a c a t t a a g a a a t c a a a c a t a c a g a c a a c g a t a a t a c a

7680 (in frame with gp41 peptide) VIREGASYGGIGGKRKFTLR cPPT/CTS VIREGASYGGIGGKRKFTLR c t a c t a t t c t t t c c c c t g c a c t g t a c c c c c c a a t c c c c c c t t t t c t t t t a a a a g t t a a c c g a t g a t a a g a a a g g g g a c g t g a c a t g g g g g g t t a g g g g g g a a a a g a a a a t t t t c a a t t g g

7740 (in frame with gp41 peptide) YRRSGNVLRISRDRDREGGE YRRSGNVLRISRDRDREGGE g a t a c c g t c g a g a t c c g t t c a c t a a t c g a a t g g a t c t g t c t c t g t c t c t c t c t c c a c c t t c t a t g g c a g c t c t a g g c a a g t g a t t a g c t t a c c t a g a c a g a g a c a g a g a g a g a g g t g g a a

PpuMIKflI 7800 (in frame with gp41 peptide) EEIGEPRDPGRPTPLHTQFS EEIGEPRDPGRPTPLHTQFS c t t c t t c t a t t c c t t c g g g c c t g t c g g g t c c c c t c g g g g t t g g g a g g t g g g t c t g a a a c g g a a g a a g a t a a g g a a g c c c g g a c a g c c c a g g g g a g c c c c a a c c c t c c a c c c a g a c t t t g c

7860 (in frame with gp41 peptide) LPSYGQRVRNVISLVAFVIR LPSYGQRVRNVISLVAFVIR a t a a t g g t g a a t a t c c c t g c c t a a c t c t a t t c a c t a t a g a a a g t a c a g c a a a a a c t a t t c t a t t a c c a c t t a t a g g g a c g g a t t g a g a t a a g t g a t a t c t t t c a t g t c g t t t t t g a t a a g

7920 (in frame with gp41 peptide) LGVLGGVIMIFLKIYWLWNT LGVLGGVIMIFLKIYWLWNT t t a a a c c t a c c a a g c c t c c t a c t a t c a t t a t g a a t a a t t t t a t a t a c c a c a g c c a a t t t g a a t t t g g a t g g t t c g g a g g a t g a t a g t a a t a c t t a t t a a a a t a t a t g g t g t c g g t t a a a c

7980 (in frame with gp41 peptide) INFWNW 15 10 5 LSAWKDLELLEQEN gp41 peptide INFWNWLSAWKDLELLEQEN t t a t g t t a a a c c a a t t c c a c a a a c t t g c c c a t t t a t c t a a t t c c a a t a a t t c t t g t t c a t a a t a c a a t t t g g t t a a g g t g t t t g a a c g g g t a a a t a g a t t a a g g t t a t t a a g a a c a a g t a

8040 1 K gp41 peptide KEQQNQSEEILSHILSTYNN t c t t t t c t t g c t g g t t t t g c g a t t c t t c a a t t a a g g a g t g t a t t a a g c t t g t g t a a t t g t a g a a a a g a a c g a c c a a a a c g c t a a g a a g t t a a t t c c t c a c a t a a t t c g a a c a c a t t a a c a

8100 IERDWEMWTTHNWIQELSKN t a a t t t c t c t g t c c c a c t c c a t c c a g g t c g t g t g a t t c c a a a t c t g t t c c a g a g a t t t a t a t t a a a g a g a c a g g g t g a g g t a g g t c c a g c a c a c t a a g g t t t a g a c a a g g t c t c t a a a t a

8160 SWSANWPVATTCILKGSCGW t a c t c c a a c t a g c a t t c c a a g g c a c a g c a g t g g t g c a a a t g a g t t t t c c a g a g c a a c c c c a t g a g g t t g a t c g t a a g g t t c c g t g t c g t c a c c a c g t t t a c t c a a a a g g t c t c g t t g g g g

8220 RRE IGLLQQDKLYREVALIRAQL a a a t c c c c a g g a g c t g t t g a t c c t t t a g g t a t c t t t c c a c a g c c a g g a t t c t t g c c t g g a t t t a g g g g t c c t c g a c a a c t a g g a a a t c c a t a g a a a g g t g t c g g t c c t a a g a a c g g a c c t

8280 RRE QKIGWVTLQLLHQQAEIARL g c t g c t t g a t g c c c c a g a c t g t g a g t t g c a a c a g a t g c t g t t g c g c c t c a a t a g c c c t c a c g a c g a a c t a c g g g g t c t g a c a c t c a a c g t t g t c t a c g a c a a c g c g g a g t t a t c g g g a g t

8340 RRE LNNQQQVIGSLLQRAQVTLT g c a a a t t g t t c t g c t g c t g c a c t a t a c c a g a c a a t a a t t g t c t g g c c t g t a c c g t c a g c g c g t t t a a c a a g a c g a c g a c g t g a t a t g g t c t g t t a t t a a c a g a c c g g a c a t g g c a g t c g c

8400 RRE MSAAGMTSGAAGLFGLFLAG t c a t t g a c g c t g c g c c c a t a g t g c t t c c t g c t g c t c c c a a g a a c c c a a g g a a c a a a g c t c a g t a a c t g c g a c g c g g g t a t c a c g a a g g a c g a c g a g g g t t c t t g g g t t c c t t g t t t c g a g

8460 RRE IGVARKERQVVRRKAKTPAV c t a t t c c c a c t g c t c t t t t t t c t c t c t g c a c c a c t c t t c t c t t t g c c t t g g t g g g t g c t a g a t a a g g g t g a c g a g a a a a a a g a g a g a c g t g g t g a g a a g a g a a a c g g a a c c a c c c a c g a t

8520 GLPEIKVVKYKYLESRWNDR c t c c t a a t g g t t c a a t t t t t a c t a c t t t a t a t t t a t a t a a t t c a c t t c t c c a a t t g t c c c g a g g a t t a c c a a g t t a a a a a t g a t g a a a t a t a a a t a t a t t a a g t g a a g a g g t t a a c a g g g

NotI 8580 M t c a t a t c t c c t c c t c c a g g t c t g a a g a t c a g c g g c c g c t t g c t g t g c g g t g g t c t t a c t t a g t a t a g a g g a g g a g g t c c a g a c t t c t a g t c g c c g g c g a a c g a c a c g c c a c c a g a a t g a a

8640 t t g t t t t g c t c t t c c t c t a t c t t g t c t a a a g c t t c c t t g g t g t c t t t t a t c t c t a t c c t t a a c a a a a c g a g a a g g a g a t a g a a c a g a t t t c g a a g g a a c c a c a g a a a a t a g a g a t a g g a a

8700 t g a t g c a c a c a a t a g a g g g t t g c t a c t g t a t t a t a t a a t g a t c t a a g t t c t t c t g a t c c t a c t a c g t g t g t t a t c t c c c a a c g a t g a c a t a a t a t a t t a c t a g a t t c a a g a a g a c t a g g a

8760 g t c t g a a g g g a t g g t t g t a g c t g t c c c a g t a t t t g t c t a c a g c c t t c t g a t g t t t c t a a c c a g a c t t c c c t a c c a a c a t c g a c a g g g t c a t a a a c a g a t g t c g g a a g a c t a c a a a g a t t g

8820 a g g c c a g g a t t a a c t g c g a a t c g t t c t a g c t c c c t g c t t g c c c a t a c t a t a t g t t t t a a t t c c g g t c c t a a t t g a c g c t t a g c a a g a t c g a g g g a c g a a c g g g t a t g a t a t a c a a a a t t a

NruI* 8880 t t a t a t t t t t t c t t t c c c c c t g g c c t t a a c c g a a t t t t t t c c c a t c g c g a t c t a a t t c t c a a t a t a a a a a a g a a a g g g g g a c c g g a a t t g g c t t a a a a a a g g g t a g c g c t a g a t t a a g a g

8940 HIV-1 Ψ c c c c g c t t a a t a c t g a c g c t c t c g c a c c c a t c t c t c t c c t t c t a g c c t c c g c t a g t c a a a g g g g c g a a t t a t g a c t g c g a g a g c g t g g g t a g a g a g a g g a a g a t c g g a g g c g a t c a g t t t

9000 HIV-1 Ψ a t t t t t g g c g t a c t c a c c a g t c g c c g c c c c t c g c c t c t t g c c g t g c g c g c t t c a g c a a g c t a a a a a c c g c a t g a g t g g t c a g c g g c g g g g a g c g g a g a a c g g c a c g c g c g a a g t c g t t c g

9060 HIV-1 Ψ c g a g t c c t g c g t c g a g a g a g c t c c t c t g g t t t c c c t t t c g c t t t c a a g t c c c t g t t c g g g g c t c a g g a c g c a g c t c t c t c g a g g a g a c c a a a g g g a a a g c g a a a g t t c a g g g a c a a g c c c

9120 5' LTR (truncated) c g c c a c t g c t a g a g a t t t t c c a c a c t g a c t a a a a g g g t c t g a g g g a t c t c t a g t t a c c a g g c g g t g a c g a t c t c t a a a a g g t g t g a c t g a t t t t c c c a g a c t c c c t a g a g a t c a a t g g t c

9180 5' LTR (truncated) a g t c a c a c a a c a g a c g g g c a c a c a c t a c t t g a a g c a c t c a a g g c a a g c t t t a t t g a g g c t t c a g t g t g t t g t c t g c c c g t g t g t g a t g a a c t t c g t g a g t t c c g t t c g a a a t a a c t c c g a

9240 5' LTR (truncated) t a a g c a g t g g g t t c c c t a g t t a g c c a g a g a g c t c c c a g g c t c a g a t c t g g t c t a a c c a g a a t t c g t c a c c c a a g g g a t c a a t c g g t c t c t c g a g g g t c c g a g t c t a g a c c a g a t t g g t c t

9300 5' LTR (truncated) CMV promoter g a g a c c c c g g t t c a c t a a a c g a g c t c t g c t t a t a t a g a c c t c c c a c c g t a c a c g c c t a c c c t c t g g g g c c a a g t g a t t t g c t c g a g a c g a a t a t a t c t g g a g g g t g g c a t g t g c g g a t g g

9360 CMV promoter g c c c a t t t g c g t c a a t g g g g c g g a g t t g t t a c g a c a t t t t g g a a a g t c c c g t t g a t t t t g c g g g t a a a c g c a g t t a c c c c g c c t c a a c a a t g c t g t a a a a c c t t t c a g g g c a a c t a a a a c

9420 CMV promoter g t g c c a a a a c a a a c t c c c a t t g a c g t c a a t g g g g t g g a g a c t t g g a a a t c c c c g t g a g t c c a c g g t t t t g t t t g a g g g t a a c t g c a g t t a c c c c a c c t c t g a a c c t t t a g g g g c a c t c a g

9480 CMV promoter CMV enhancer a a a c c g c t a t c c a c g c c c a t t g a t g t a c t g c c a a a a c c g c a t c a c c a t g g t a a t a g c g a t t t t g g c g a t a g g t g c g g g t a a c t a c a t g a c g g t t t t g g c g t a g t g g t a c c a t t a t c g c t a

SnaBI 9540 CMV enhancer g a c t a a t a c g t a g a t g t a c t g c c a a g t a g g a a a g t c c c a t a a g g t c a t g t a c t g g g c a t a c t g a t t a t g c a t c t a c a t g a c g g t t c a t c c t t t c a g g g t a t t c c a g t a c a t g a c c c g t a t

NdeI 9600 CMV enhancer a t g c c a g g c g g g c c a t t t a c c g t c a t t g a c g t c a a t a g g g g g c g t a c t t g g c a t a t g a t a t a c g g t c c g c c c g g t a a a t g g c a g t a a c t g c a g t t a t c c c c c g c a t g a a c c g t a t a c t a t

9660 CMV enhancer c a c t t g a t g t a c t g c c a a g t g g g c a g t t t a c c g t a a a t a c t c c a c c c a t t g a c g t c a a t g g t g a a c t a c a t g a c g g t t c a c c c g t c a a a t g g c a t t t a t g a g g t g g g t a a c t g c a g t t a c

9720 CMV enhancer g a a a g t c c c t a t t g g c g t t a c t a t g g g a a c a t a c g t c a t t a t t g a c g t c a a t g g g c g g g g c t t t c a g g g a t a a c c g c a a t g a t a c c c t t g t a t g c a g t a a t a a c t g c a g t t a c c c g c c c c

9780 CMV enhancer g t c g t t g g g c g g t c a g c c a g g c g g g c c a t t t a c c g t a a g t t a t g t a a c g c g g a a c t c c a t c a g c a a c c c g c c a g t c g g t c c g c c c g g t a a a t g g c a t t c a a t a c a t t g c g c c t t g a g g t a

9840 CMV enhancer a t a t g g g c t a t g a a c t a a t g a c c c c g t a a t t g a t t a c t a t t a a t a a c t a g t c a a t a a t c a t a t a c c c g a t a c t t g a t t a c t g g g g c a t t a a c t a a t g a t a a t t a t t g a t c a g t t a t t a g t

9900 CMV enhancer a t g t c a a c a t g g c g g t a a t g t t g g a c a t g a g c c a a t a t a a a t g t a c a t a t t a t g a t a t g g t a c a g t t g t a c c g c c a t t a c a a c c t g t a c t c g g t t a t a t t t a c a t g t a t a a t a c t a t a c c

9960 T3 promoter G G A A A T C A C T C C C A A T3 a t a c a a c g t a t g c a a t g g c c a a g c t t g c a g c t c c a g c t t t t g t t c c c t t t a g t g a g g g t t t a t g t t g c a t a c g t t a c c g g t t c g a a c g t c g a g g t c g a a a a c a a g g g a a a t c a c t c c c a a

10,020 T3 promoter M13 rev lac operator T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a

10,080 -10 -35 lac operator lac promoter CAP binding site c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c

10,140 CAP binding site a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a

10,200 g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c

10,260 G A G C G A G T G A C T G A G C G A L4440 g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g

10,320 g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c

PciI 10,380 a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a

10,386 g g c g t t c c g c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.