Addgene pHAGE-PIK3CA-E39K Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pHAGE-PIK3CA-E39K Sequencing Result


Map View

M13 rev M13 Reverse (11,420 .. 11,436) lac operator M13/pUC Reverse (11,401 .. 11,423) CAP binding site SfiI (11,199) L4440 (11,065 .. 11,082) DrdI (10,865) pBR322ori-F (10,812 .. 10,831) FspI (9856) PvuI (9710) HincII (9537) Amp-R (9510 .. 9529) pBRforEco (9101 .. 9119) PacI (9064) SmaI (9056) TspMI - XmaI (9054) WPRE-R (8139 .. 8159) BspDI - ClaI (8074) CsiI - SexAI * (8040) Puro-F (7969 .. 7989) BsmBI - Esp3I (7865) BstEII (7607) RsrII (7589) BsiWI (7529) PflFI - Tth111I (7515) Puro-R (7473 .. 7492) NdeI (7471) BmgBI (7429) KpnI (7331) Acc65I (7327) IRES-F (7280 .. 7299) PaqCI (7225) IRES reverse (7053 .. 7070) MluI (6869) PspXI (6802) NotI (6796) T7 (6731 .. 6750) T7 promoter T3 promoter NsiI (6645) EBV-rev (11,486 .. 11,505) NruI * (833) KflI (1934) SpeI (2212) EF1a-F (3349 .. 3369) BstZ17I (4067) NcoI (4530) Bsu36I (5737) pHAGE-PIK3CA-E39K 11,696 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t

120 3' LTR c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g

180 3' LTR t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t

240 3' LTR a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c

300 3' LTR a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c

360 3' LTR a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c

420 3' LTR c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a

480 3' LTR c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

540 3' LTR g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

600 3' LTR t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

660 3' LTR a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

720 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

780 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

NruI* 840 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

900 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

960 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1020 *I g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1080 MIYYLLLGRNHADFSLSLLC c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1140 WPLKLCSLPLAFCFYSWRVA c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

1200 MRDNWRS LPRGSIKLGPPPSILSLQLL a a g c g g c c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c

1260 ELYKYKVVKIEPLGVAPTKA SNYLYLTTFISGNPTAGVLA t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g

1320 RRE KRRVVQREKRAVGIGALFLG FLLTTCLSFLATPIPAKNRP a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c

1380 RRE FLGAAGSTMGAASMTLTVQA NKPAAPLVIPAADIVSVTCA g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g

1440 RRE RQLLSGIVQQQNNLLRAIEA LCNNDPITCCCFLKSLAISA c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g

1500 RRE QQHLLQLTVWGIKQLQARIL CCCRNCSVTQPM g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a

1560 RRE AVERYLKDQQLLGIWGCSGK g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t

1620 LICTTAVPWNASWSNKSLEQ a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t

1680 IWNHTTWMEWDREINNYTSL g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a

1740 1 5 KNEQELL gp41 peptide IHSLIEESQNQQEKNEQELL a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a

1800 10 15 ELDKWASL (in frame with gp41 peptide) WNWFNITNWLWY gp41 peptide ELDKWASLWNWFNITNWLWY g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t

1860 (in frame with gp41 peptide) IKLFIMIVGGLVGLRIVFAV IKLFIMIVGGLVGLRIVFAV t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a

1920 (in frame with gp41 peptide) LSIVNRVRQGYSPLSFQTHL LSIVNRVRQGYSPLSFQTHL a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a

KflI 1980 (in frame with gp41 peptide) PTPRGPDRPEGIEEEGGERD PTPRGPDRPEGIEEEGGERD c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t

2040 (in frame with gp41 peptide) RDRSIRLVNGSRRYRRIHKW RDRSIRLVNGSRRYRRIHKW c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g c c g a a t t c a c a a a t g g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c g g c t t a a g t g t t t a c

2100 (in frame with gp41 peptide) QYSSTILKEKGGLGGTVQGK cPPT/CTS QYSSTILKEKGGLGGTVQGK g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t

2160 E* cPPT/CTS E* a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t

SpeI 2220 cPPT/CTS a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g a c t a g t c g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c t g a t c a g c a

2280 EF-1 α promoter g a g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g c t c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c

2340 EF-1 α promoter g g g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a c c c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t

2400 EF-1 α promoter g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c

2460 EF-1 α promoter EF-1 α intron A c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c

2520 EF-1 α promoter EF-1 α intron A c c g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a g g c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t

2580 EF-1 α promoter EF-1 α intron A *KWRAATRSEQDRAEPQFHT t t a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t a a t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a

2640 EF-1 α promoter EF-1 α intron A PSNSAKRKLLGKAEHKLQPR g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a

2700 EF-1 α promoter EF-1 α intron A AQASPGGRAFRTAGERRDRQ g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c

2760 EF-1 α promoter EF-1 α intron A KRYTELWKFNKIVQQSAKKR c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a

2820 EF-1 α promoter EF-1 α intron A ALYDQLHPGLDACQYKPKQP g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c

2880 EF-1 α promoter EF-1 α intron A RPRRRPGHTGACMNPSAPGA c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g

2940 EF-1 α promoter EF-1 α intron A LAAVSFRVPTTELQGAQEPA g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g

3000 EF-1 α promoter EF-1 α intron A QGRAATYRGARPPLAPGTPV c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g

3060 EF-1 α promoter EF-1 α intron A LQTLPFIAAERGQQLSSLIS c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t

3120 EF-1 α promoter EF-1 α intron A SAASPLAPPHTVWVFSFPRE g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g

3180 EF-1 α promoter EF-1 α intron A TRLRRKM c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c

3240 EF-1 α promoter EF-1 α intron A a t t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t a a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t

3300 EF-1 α promoter EF-1 α intron A t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a

3360 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A EF1a-F a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t

3420 EF-1 α promoter EF-1 α intron A C A G T G G T T C EF1a-F c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a a g c g g c c c t g c a g a t a t c a g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t t c g c c g g g a c g t c t a t a g t

3480 attB1 MPPRPSSGELW a c a a g t t t g t a c a a a a a a g c a g g c a c c a t g c c t c c a a g a c c a t c a t c a g g t g a a c t g t g g t g t t c a a a c a t g t t t t t t c g t c c g t g g t a c g g a g g t t c t g g t a g t a g t c c a c t t g a c a c c

3540 GIHLMPPRILVECLLPNGMI g g c a t c c a c t t g a t g c c c c c a a g a a t c c t a g t a g a a t g t t t a c t a c c a a a t g g a a t g a t a c c g t a g g t g a a c t a c g g g g g t t c t t a g g a t c a t c t t a c a a a t g a t g g t t t a c c t t a c t a t

3600 VTLECLRKATLITIKHELFK g t g a c t t t a g a a t g c c t c c g t a a g g c t a c a t t a a t a a c c a t a a a g c a t g a a c t a t t t a a a c a c t g a a a t c t t a c g g a g g c a t t c c g a t g t a a t t a t t g g t a t t t c g t a c t t g a t a a a t t t

3660 EARKYPLHQLLQDESSYIFV g a a g c a a g a a a a t a c c c c c t c c a t c a a c t t c t t c a a g a t g a a t c t t c t t a c a t t t t c g t a c t t c g t t c t t t t a t g g g g g a g g t a g t t g a a g a a g t t c t a c t t a g a a g a a t g t a a a a g c a t

3720 SVTQEAEREEFFDETRRLCD a g t g t t a c t c a a g a a g c a g a a a g g g a a g a a t t t t t t g a t g a a a c a a g a c g a c t t t g t g a c t c a c a a t g a g t t c t t c g t c t t t c c c t t c t t a a a a a a c t a c t t t g t t c t g c t g a a a c a c t g

3780 LRLFQPFLKVIEPVGNREEK c t t c g g c t t t t t c a a c c c t t t t t a a a a g t a a t t g a a c c a g t a g g c a a c c g t g a a g a a a a g g a a g c c g a a a a a g t t g g g a a a a a t t t t c a t t a a c t t g g t c a t c c g t t g g c a c t t c t t t t c

3840 ILNREIGFAIGMPVCEFDMV a t c c t c a a t c g a g a a a t t g g t t t t g c t a t c g g c a t g c c a g t g t g t g a a t t t g a t a t g g t t t a g g a g t t a g c t c t t t a a c c a a a a c g a t a g c c g t a c g g t c a c a c a c t t a a a c t a t a c c a a

3900 KDPEVQDFRRNILNVCKEAV a a a g a t c c a g a a g t a c a g g a c t t c c g a a g a a a t a t t c t g a a c g t t t g t a a a g a a g c t g t g t t t c t a g g t c t t c a t g t c c t g a a g g c t t c t t t a t a a g a c t t g c a a a c a t t t c t t c g a c a c

3960 DLRDLNSPHSRAMYVYPPNV g a t c t t a g g g a c c t c a a t t c a c c t c a t a g t a g a g c a a t g t a t g t c t a t c c t c c a a a t g t a c t a g a a t c c c t g g a g t t a a g t g g a g t a t c a t c t c g t t a c a t a c a g a t a g g a g g t t t a c a t

4020 ESSPELPKHIYNKLDKGQII g a a t c t t c a c c a g a a t t g c c a a a g c a c a t a t a t a a t a a a t t a g a t a a a g g g c a a a t a a t a c t t a g a a g t g g t c t t a a c g g t t t c g t g t a t a t a t t a t t t a a t c t a t t t c c c g t t t a t t a t

BstZ17I 4080 VVIWVIVSPNNDKQKYTLKI g t g g t g a t c t g g g t a a t a g t t t c t c c a a a t a a t g a c a a g c a g a a g t a t a c t c t g a a a a t c c a c c a c t a g a c c c a t t a t c a a a g a g g t t t a t t a c t g t t c g t c t t c a t a t g a g a c t t t t a g

4140 NHDCVPEQVIAEAIRKKTRS a a c c a t g a c t g t g t a c c a g a a c a a g t a a t t g c t g a a g c a a t c a g g a a a a a a a c t c g a a g t t t g g t a c t g a c a c a t g g t c t t g t t c a t t a a c g a c t t c g t t a g t c c t t t t t t t g a g c t t c a

4200 MLLSSEQLKLCVLEYQGKYI a t g t t g c t a t c c t c t g a a c a a c t a a a a c t c t g t g t t t t a g a a t a t c a g g g c a a g t a t a t t t a c a a c g a t a g g a g a c t t g t t g a t t t t g a g a c a c a a a a t c t t a t a g t c c c g t t c a t a t a a

4260 LKVCGCDEYFLEKYPLSQYK t t a a a a g t g t g t g g a t g t g a t g a a t a c t t c c t a g a a a a a t a t c c t c t g a g t c a g t a t a a g a a t t t t c a c a c a c c t a c a c t a c t t a t g a a g g a t c t t t t t a t a g g a g a c t c a g t c a t a t t c

4320 YIRSCIMLGRMPNLMLMAKE t a t a t a a g a a g c t g t a t a a t g c t t g g g a g g a t g c c c a a t t t g a t g t t g a t g g c t a a a g a a a t a t a t t c t t c g a c a t a t t a c g a a c c c t c c t a c g g g t t a a a c t a c a a c t a c c g a t t t c t t

4380 SLYSQLPMDCFTMPSYSRRI a g c c t t t a t t c t c a a c t g c c a a t g g a c t g t t t t a c a a t g c c a t c t t a t t c c a g a c g c a t t t c g g a a a t a a g a g t t g a c g g t t a c c t g a c a a a a t g t t a c g g t a g a a t a a g g t c t g c g t a a

4440 STATPYMNGETSTKSLWVIN t c c a c a g c t a c a c c a t a t a t g a a t g g a g a a a c a t c t a c a a a a t c c c t t t g g g t t a t a a a t a g g t g t c g a t g t g g t a t a t a c t t a c c t c t t t g t a g a t g t t t t a g g g a a a c c c a a t a t t t a

4500 SALRIKILCATYVNVNIRDI a g t g c a c t c a g a a t a a a a a t t c t t t g t g c a a c c t a c g t g a a t g t a a a t a t t c g a g a c a t t t c a c g t g a g t c t t a t t t t t a a g a a a c a c g t t g g a t g c a c t t a c a t t t a t a a g c t c t g t a a

NcoI 4560 DKIYVRTGIYHGGEPLCDNV g a t a a g a t c t a t g t t c g a a c a g g t a t c t a c c a t g g a g g a g a a c c c t t a t g t g a c a a t g t g c t a t t c t a g a t a c a a g c t t g t c c a t a g a t g g t a c c t c c t c t t g g g a a t a c a c t g t t a c a c

4620 NTQRVPCSNPRWNEWLNYDI a a c a c t c a a a g a g t a c c t t g t t c c a a t c c c a g g t g g a a t g a a t g g c t g a a t t a t g a t a t a t t g t g a g t t t c t c a t g g a a c a a g g t t a g g g t c c a c c t t a c t t a c c g a c t t a a t a c t a t a t

4680 YIPDLPRAARLCLSICSVKG t a c a t t c c t g a t c t t c c t c g t g c t g c t c g a c t t t g c c t t t c c a t t t g c t c t g t t a a a g g c a t g t a a g g a c t a g a a g g a g c a c g a c g a g c t g a a a c g g a a a g g t a a a c g a g a c a a t t t c c g

4740 RKGAKEEHCPLAWGNINLFD c g a a a g g g t g c t a a a g a g g a a c a c t g t c c a t t g g c a t g g g g a a a t a t a a a c t t g t t t g a t g c t t t c c c a c g a t t t c t c c t t g t g a c a g g t a a c c g t a c c c c t t t a t a t t t g a a c a a a c t a

4800 YTDTLVSGKMALNLWPVPHG t a c a c a g a c a c t c t a g t a t c t g g a a a a a t g g c t t t g a a t c t t t g g c c a g t a c c t c a t g g a a t g t g t c t g t g a g a t c a t a g a c c t t t t t a c c g a a a c t t a g a a a c c g g t c a t g g a g t a c c t

4860 LEDLLNPIGVTGSNPNKETP t t a g a a g a t t t g c t g a a c c c t a t t g g t g t t a c t g g a t c a a a t c c a a a t a a a g a a a c t c c a a a t c t t c t a a a c g a c t t g g g a t a a c c a c a a t g a c c t a g t t t a g g t t t a t t t c t t t g a g g t

4920 CLELEFDWFSSVVKFPDMSV t g c t t a g a g t t g g a g t t t g a c t g g t t c a g c a g t g t g g t a a a g t t c c c a g a t a t g t c a g t g a c g a a t c t c a a c c t c a a a c t g a c c a a g t c g t c a c a c c a t t t c a a g g g t c t a t a c a g t c a c

4980 IEEHANWSVSREAGFSYSHA a t t g a a g a g c a t g c c a a t t g g t c t g t a t c c c g a g a a g c a g g a t t t a g c t a t t c c c a c g c a t a a c t t c t c g t a c g g t t a a c c a g a c a t a g g g c t c t t c g t c c t a a a t c g a t a a g g g t g c g t

5040 GLSNRLARDNELRENDKEQL g g a c t g a g t a a c a g a c t a g c t a g a g a c a a t g a a t t a a g g g a a a a t g a c a a a g a a c a g c t c c c t g a c t c a t t g t c t g a t c g a t c t c t g t t a c t t a a t t c c c t t t t a c t g t t t c t t g t c g a g

5100 KAISTRDPLSEITEQEKDFL a a a g c a a t t t c t a c a c g a g a t c c t c t c t c t g a a a t c a c t g a g c a g g a g a a a g a t t t t c t a t t t c g t t a a a g a t g t g c t c t a g g a g a g a g a c t t t a g t g a c t c g t c c t c t t t c t a a a a g a t

5160 WSHRHYCVTIPEILPKLLLS t g g a g t c a c a g a c a c t a t t g t g t a a c t a t c c c c g a a a t t c t a c c c a a a t t g c t t c t g t c t a c c t c a g t g t c t g t g a t a a c a c a t t g a t a g g g g c t t t a a g a t g g g t t t a a c g a a g a c a g a

5220 VKWNSRDEVAQMYCLVKDWP g t t a a a t g g a a t t c t a g a g a t g a a g t a g c c c a g a t g t a t t g c t t g g t a a a a g a t t g g c c t c a a t t t a c c t t a a g a t c t c t a c t t c a t c g g g t c t a c a t a a c g a a c c a t t t t c t a a c c g g a

5280 PIKPEQAMELLDCNYPDPMV c c a a t c a a a c c t g a a c a g g c t a t g g a a c t t c t g g a c t g t a a t t a c c c a g a t c c t a t g g t t g g t t a g t t t g g a c t t g t c c g a t a c c t t g a a g a c c t g a c a t t a a t g g g t c t a g g a t a c c a a

5340 RGFAVRCLEKYLTDDKLSQY c g a g g t t t t g c t g t t c g g t g c t t g g a a a a a t a t t t a a c a g a t g a c a a a c t t t c t c a g t a t g c t c c a a a a c g a c a a g c c a c g a a c c t t t t t a t a a a t t g t c t a c t g t t t g a a a g a g t c a t a

5400 LIQLVQVLKYEQYLDNLLVR t t a a t t c a g c t a g t a c a g g t c c t a a a a t a t g a a c a a t a t t t g g a t a a c t t g c t t g t g a g a a a t t a a g t c g a t c a t g t c c a g g a t t t t a t a c t t g t t a t a a a c c t a t t g a a c g a a c a c t c t

5460 FLLKKALTNQRIGHFFFWHL t t t t t a c t g a a g a a a g c a t t g a c t a a t c a a a g g a t t g g g c a c t t t t t c t t t t g g c a t t t a a a a a a t g a c t t c t t t c g t a a c t g a t t a g t t t c c t a a c c c g t g a a a a a g a a a a c c g t a a a t

5520 KSEMHNKTVSQRFGLLLESY a a a t c t g a g a t g c a c a a t a a a a c a g t t a g c c a g a g g t t t g g c c t g c t t t t g g a g t c c t a t t t t a g a c t c t a c g t g t t a t t t t g t c a a t c g g t c t c c a a a c c g g a c g a a a a c c t c a g g a t a

5580 CRACGMYLKHLNRQVEAMEK t g t c g t g c a t g t g g g a t g t a t t t g a a g c a c c t g a a t a g g c a a g t c g a g g c a a t g g a a a a g a c a g c a c g t a c a c c c t a c a t a a a c t t c g t g g a c t t a t c c g t t c a g c t c c g t t a c c t t t t c

5640 LINLTDILKQEKKDETQKVQ c t c a t t a a c t t a a c t g a c a t t c t c a a a c a g g a g a a g a a g g a t g a a a c a c a a a a g g t a c a g g a g t a a t t g a a t t g a c t g t a a g a g t t t g t c c t c t t c t t c c t a c t t t g t g t t t t c c a t g t c

5700 MKFLVEQMRRPDFMDALQGF a t g a a g t t t t t a g t t g a g c a a a t g a g g c g a c c a g a t t t c a t g g a t g c t c t a c a g g g c t t t t a c t t c a a a a a t c a a c t c g t t t a c t c c g c t g g t c t a a a g t a c c t a c g a g a t g t c c c g a a a

Bsu36I 5760 LSPLNPAHQLGNLRLEECRI c t g t c t c c t c t a a a c c c t g c t c a t c a a c t a g g a a a c c t c a g g c t t g a a g a g t g t c g a a t t g a c a g a g g a g a t t t g g g a c g a g t a g t t g a t c c t t t g g a g t c c g a a c t t c t c a c a g c t t a a

5820 MSSAKRPLWLNWENPDIMSE a t g t c c t c t g c a a a a a g g c c a c t g t g g t t g a a t t g g g a g a a c c c a g a c a t c a t g t c a g a g t a c a g g a g a c g t t t t t c c g g t g a c a c c a a c t t a a c c c t c t t g g g t c t g t a g t a c a g t c t c

5880 LLFQNNEIIFKNGDDLRQDM t t a c t g t t t c a g a a c a a t g a g a t c a t c t t t a a a a a t g g g g a t g a t t t a c g g c a a g a t a t g a a t g a c a a a g t c t t g t t a c t c t a g t a g a a a t t t t t a c c c c t a c t a a a t g c c g t t c t a t a c

5940 LTLQIIRIMENIWQNQGLDL c t a a c a c t t c a a a t t a t t c g t a t t a t g g a a a a t a t c t g g c a a a a t c a a g g t c t t g a t c t t g a t t g t g a a g t t t a a t a a g c a t a a t a c c t t t t a t a g a c c g t t t t a g t t c c a g a a c t a g a a

6000 RMLPYGCLSIGDCVGLIEVV c g a a t g t t a c c t t a t g g t t g t c t g t c a a t c g g t g a c t g t g t g g g a c t t a t t g a g g t g g t g g c t t a c a a t g g a a t a c c a a c a g a c a g t t a g c c a c t g a c a c a c c c t g a a t a a c t c c a c c a c

6060 RNSHTIMQIQCKGGLKGALQ c g a a a t t c t c a c a c t a t t a t g c a a a t t c a g t g c a a a g g c g g c t t g a a a g g t g c a c t g c a g g c t t t a a g a g t g t g a t a a t a c g t t t a a g t c a c g t t t c c g c c g a a c t t t c c a c g t g a c g t c

6120 FNSHTLHQWLKDKNKGEIYD t t c a a c a g c c a c a c a c t a c a t c a g t g g c t c a a a g a c a a g a a c a a a g g a g a a a t a t a t g a t a a g t t g t c g g t g t g t g a t g t a g t c a c c g a g t t t c t g t t c t t g t t t c c t c t t t a t a t a c t a

6180 AAIDLFTRSCAGYCVATFIL g c a g c c a t t g a c c t g t t t a c a c g t t c a t g t g c t g g a t a c t g t g t a g c t a c c t t c a t t t t g c g t c g g t a a c t g g a c a a a t g t g c a a g t a c a c g a c c t a t g a c a c a t c g a t g g a a g t a a a a c

6240 GIGDRHNSNIMVKDDGQLFH g g a a t t g g a g a t c g t c a c a a t a g t a a c a t c a t g g t g a a a g a c g a t g g a c a a c t g t t t c a t c c t t a a c c t c t a g c a g t g t t a t c a t t g t a g t a c c a c t t t c t g c t a c c t g t t g a c a a a g t a

6300 IDFGHFLDHKKKKFGYKRER a t a g a t t t t g g a c a c t t t t t g g a t c a c a a g a a g a a a a a a t t t g g t t a t a a a c g a g a a c g t t a t c t a a a a c c t g t g a a a a a c c t a g t g t t c t t c t t t t t t a a a c c a a t a t t t g c t c t t g c a

6360 VPFVLTQDFLIVISKGAQEC g t g c c a t t t g t t t t g a c a c a g g a t t t c t t a a t a g t g a t t a g t a a a g g a g c c c a a g a a t g c c a c g g t a a a c a a a a c t g t g t c c t a a a g a a t t a t c a c t a a t c a t t t c c t c g g g t t c t t a c g

6420 TKTREFERFQEMCYKAYLAI a c a a a g a c a a g a g a a t t t g a g a g g t t t c a g g a g a t g t g t t a c a a g g c t t a t c t a g c t a t t t g t t t c t g t t c t c t t a a a c t c t c c a a a g t c c t c t a c a c a a t g t t c c g a a t a g a t c g a t a a

6480 RQHANLFINLFSMMLGSGMP c g a c a g c a t g c c a a t c t c t t c a t a a a t c t t t t c t c a a t g a t g c t t g g c t c t g g a a t g c c a g c t g t c g t a c g g t t a g a g a a g t a t t t a g a a a a g a g t t a c t a c g a a c c g a g a c c t t a c g g t

6540 ELQSFDDIAYIRKTLALDKT g a a c t a c a a t c t t t t g a t g a c a t t g c a t a c a t t c g a a a g a c c c t a g c c t t a g a t a a a a c t c t t g a t g t t a g a a a a c t a c t g t a a c g t a t g t a a g c t t t c t g g g a t c g g a a t c t a t t t t g a

6600 EQEALEYFMKQMNDAHHGGW g a g c a a g a g g c t t t g g a g t a t t t c a t g a a a c a a a t g a a t g a t g c a c a t c a t g g t g g c t g g c t c g t t c t c c g a a a c c t c a t a a a g t a c t t t g t t t a c t t a c t a c g t g t a g t a c c a c c g a c c

NsiI 6660 attB2 TTKMDWIFHTIKQHALN* a c a a c a a a a a t g g a t t g g a t c t t c c a c a c a a t t a a a c a g c a t g c a t t g a a c t a g a a c c c a t g t t g t t t t t a c c t a a c c t a g a a g g t g t g t t a a t t t g t c g t a c g t a a c t t g a t c t t g g g t

6720 attB2 T3 promoter g c t t t c t t g t a c a a a g t g g t g a t a t c c a a t t a a c c c t c a c t a a a g g t g t t a a a t g t a t g t c g a a a g a a c a t g t t t c a c c a c t a t a g g t t a a t t g g g a g t g a t t t c c a c a a t t t a c a t a c a

6780 T7 promoter attB4 G G G A T A T C A C T C A G C A T A A T T7 a g t a a t t g a g c c c t a t a g t g a g t c g t a t t a c c a c c c a a c t t t t c t a t a c a a a g t g g t t g a t c a t t a a c t c g g g a t a t c a c t c a g c a t a a t g g t g g g t t g a a a a g a t a t g t t t c a c c a a c t

PspXINotI 6840 1 GKPI V5 tag t a t c c a g c a c a g t g g c g g c c g c t c g a g t c t a g a g g g c c c g c g g t t c g a a g g t a a g c c t a t a t a g g t c g t g t c a c c g c c g g c g a g c t c a g a t c t c c c g g g c g c c a a g c t t c c a t t c g g a t a

MluI 6900 5 10 PNPLLGLDST RTG* V5 tag IRES c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g c g t a c c g g t t a g t a a t g a g a t c c c t c c c c g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c g c a t g g c c a a t c a t t a c t c t a g g g a g g g g

6960 IRES IRES c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a

7020 IRES IRES g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a

7080 IRES IRES G A G A G C G G T T T C C T T A C G IRES reverse c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a

7140 IRES IRES g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g

7200 IRES IRES g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g

PaqCI 7260 IRES IRES a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a

7320 IRES IRES T G G C T C T C C T C A A G C G T A T T IRES-F a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g

Acc65IKpnI 7380 IRES IRES c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t g c a c a t g c t t t a c a t g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a c g t g t a c g a a a t g t a c

BmgBI 7440 IRES IRES t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a

NdeI 7500 1 5 MTEYKPTVR IRES PuroR IRES T A C T G G C T C A T G T T C G G G T G Puro-R MATHMTEYKPTVR t t g a a a a a c a c g a t g a t a a t a t g g c c a c a c a t a t g a c c g a g t a c a a g c c c a c g g t g c g c c a a c t t t t t g t g c t a c t a t t a t a c c g g t g t g t a t a c t g g c t c a t g t t c g g g t g c c a c g c g g

BsiWITth111IPflFI 7560 10 15 20 25 LATRDDVPRAVRTLAAAFAD PuroR LATRDDVPRAVRTLAAAFAD t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a

BstEIIRsrII 7620 30 35 40 45 YPATRHTVDPDRHIERVTEL PuroR YPATRHTVDPDRHIERVTEL a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g

7680 50 55 60 65 QELFLTRVGLDIGKVWVADD PuroR QELFLTRVGLDIGKVWVADD a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c

7740 70 75 80 85 GAAVAVWTTPESVEAGAVFA PuroR GAAVAVWTTPESVEAGAVFA g c g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g c g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c

7800 90 95 100 105 EIGPRMAELSGSRLAAQQQM PuroR EIGPRMAELSGSRLAAQQQM a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c

7860 110 115 120 125 EGLLAPHRPKEPAWFLATVG PuroR EGLLAPHRPKEPAWFLATVG a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c

BsmBIEsp3I 7920 130 135 140 145 VSPDHQGKGLGSAVVLPGVE PuroR VSPDHQGKGLGSAVVLPGVE t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c

7980 150 155 160 165 AAERAGVPAFLETSAPRNLP PuroR G C A A C C T C C C C T Puro-F AAERAGVPAFLETSAPRNLP c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a

8040 170 175 180 185 FYERLGFTVTADVEVPEGPR PuroR T C T A C G A G C Puro-F FYERLGFTVTADVEVPEGPR t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t

BspDIClaISexAI*CsiI 8100 190 195 200 TWCMTRKPGA* PuroR WPRE TWCMTRKPGA* c c t g g t g c a t g a c c c g c a a g c c c g g t g c c t a a a t c g a t a g a t c c t a a t c a a c c t c t g g a t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g a t t t a g c t a t c t a g g a t t a g t t g g a g a c c t a

8160 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a

8220 WPRE MPLYHAIASRMAFIF MLLLPVWLSFS g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g

8280 WPRE SSLYKSWLLSLYEELWPVVR PPCINPGCCLFMRSCGPLSG t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c

8340 WPRE QRGVVCTVFADATPTGWGIA NVAWCALCLLTQPPLVGALP c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g

8400 WPRE TTCQLLSGTFAFPLPIATAE PPVSSFPGLSLSPSLLPRRN a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t

8460 WPRE LIAACLARCWTGARLLGTDN SSPPALPAAGQGLGCWALTI c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a

8520 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGG SVVLSGKSSSFPWLLACVAT PWCCRGNHRPFLGCSPVLPP t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g

8580 WPRE (in frame with Factor Xa site) PNQAPRGEAVDR 1 RGEI Factor Xa site WILRGTSFCYVPSALNPADL GFCAGRPSATSLRPSIQRTF t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a

8640 WPRE PSRGLLPALRPLPRLRLRPQ LPAACCRLCGLFRVFAFALR c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c

8700 WPRE TSRISLWAASPPEIL* RVGSPFGPPPRLRSFKTNDL a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g a g a t c c t t t a a g a c c a a t g a c t t t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c t c t a g g a a a t t c t g g t t a c t g a a

8760 3' LTR ( Δ U3) QGSCRS* a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t

8820 3' LTR ( Δ U3) t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g

8880 3' LTR ( Δ U3) a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t

8940 3' LTR ( Δ U3) g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t

9000 3' LTR ( Δ U3) g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g

TspMIXmaISmaI 9060 t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g c c c g g g t a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c g g g c c c a

PacI 9120 C G G A G C A C T A T G C G G A T A A pBRforEco t a a t t a a g g a a a g g g c t a g a t c a t t c t t g a a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t a t t a a t t c c t t t c c c g a t c t a g t a a g a a c t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a

9180 t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t

9240 AmpR promoter a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g

9300 1 signal sequence MSI AmpR promoter AmpR MSI a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a

9360 5 10 15 20 signal sequence QHFRVALIPFFAAFCLPVFA AmpR QHFRVALIPFFAAFCLPVFA c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a

9420 25 30 35 40 HPETLVKVKDAEDQLGARVG AmpR HPETLVKVKDAEDQLGARVG c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a

9480 45 50 55 60 YIELDLNSGKILESFRPEER AmpR YIELDLNSGKILESFRPEER t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a

HincII 9540 65 70 75 80 FPMMSTFKVLLCGAVLSRVD AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FPMMSTFKVLLCGAVLSRVD t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g

9600 85 90 95 100 AGQEQLGRRIHYSQNDLVEY AmpR AGQEQLGRRIHYSQNDLVEY g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g

9660 105 110 115 120 SPVTEKHLTDGMTVRELCSA AmpR SPVTEKHLTDGMTVRELCSA t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a

PvuI 9720 125 130 135 140 AITMSDNTAANLLLTTIGGP AmpR AITMSDNTAANLLLTTIGGP g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c

9780 145 150 155 160 KELTAFLHNMGDHVTRLDRW AmpR KELTAFLHNMGDHVTRLDRW a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c

9840 165 170 175 180 EPELNEAIPNDERDTTMPVA AmpR EPELNEAIPNDERDTTMPVA g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t

FspI 9900 185 190 195 200 MATTLRKLLTGELLTLASRQ AmpR MATTLRKLLTGELLTLASRQ a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t

9960 205 210 215 220 QLIDWMEADKVAGPLLRSAL AmpR QLIDWMEADKVAGPLLRSAL c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a

10,020 225 230 235 240 PAGWFIADKSGAGERGSRGI AmpR PAGWFIADKSGAGERGSRGI c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g

10,080 245 250 255 260 IAALGPDGKPSRIVVIYTTG AmpR IAALGPDGKPSRIVVIYTTG a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c

10,140 265 270 275 280 SQATMDERNRQIAEIGASLI AmpR SQATMDERNRQIAEIGASLI a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a

10,200 285 KHW* AmpR KHW* a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a

10,260 c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g

10,320 c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a

10,380 ori t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t

10,440 ori c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g

10,500 ori t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g

10,560 ori t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a

10,620 ori g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a

10,680 ori a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c

10,740 ori a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t

10,800 ori g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c

10,860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t

DrdI 10,920 ori c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g

10,980 a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a

11,040 g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a

11,100 A G C G A G T C A G T G A G C G A G L4440 c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t

11,160 a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c a a g c t c a t g g c t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g t t c g a g t a c c g a

SfiI 11,220 g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t

11,280 a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c c g t g g c a c g a c t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g g c a c c g t g c t g

11,340 CAP binding site a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a

11,400 -35 -10 CAP binding site lac promoter lac operator c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c

11,460 lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c a t g a t t a c g a a t t t c a c a a a t a a a t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g t a c t a a t g c t t a a a g t g t t t a t t t

11,520 G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a

11,580 g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c c a t a c c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g g t a t g

11,640 c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t c t g a a g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a g a c t t

3ʹ 5ʹ 11,696 t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g t a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.