Addgene KZ301: pMVP (L3-L2) P2A-Blast + polyA Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: KZ301: pMVP (L3-L2) P2A-Blast + polyA Sequencing Result


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PciI (3281) pBR322ori-F (3126 .. 3145) BciVI (3083) ApaLI (2967) AlwNI (2872) PflMI (2369) Bpu10I (2123) BsmBI - Esp3I (2122) AsiSI (2106) BsrFI (2060) SspI (2031) EcoNI (2018) Kan-R (1748 .. 1767) pENTR-R (1670 .. 1689) L4440 (92 .. 109) BspQI - SapI (111) rrnB T2 terminator BsaHI (327) AclI (364) HincII - HpaI (501) M13/pUC Forward (522 .. 544) M13 Forward (536 .. 553) AhdI (549) AflII (554) AvaI - BsoBI (560) PspOMI (563) ApaI (567) BamHI (671) BspEI * (674) BseRI (734) Bsu36I (735) MscI (746) BsgI (930) BsaBI * (1041) EcoRI (1142) HindIII (1150) BlpI (1153) Eco53kI (1164) SacI (1166) BclI * (1170) BGH-rev (1177 .. 1194) BtgZI (1276) SphI (1383) Acc65I (1391) KpnI (1395) BsrGI - TatI (1411) PstI (1511) NotI (1515) EcoRV (1524) T7 (1528 .. 1547) M13 rev M13 Reverse (1552 .. 1568) KZ301: pMVP (L3-L2) P2A-Blast + polyA 3287 bp

Sequence View

60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t

BspQISapI 120 A G C G A G T C A G T G A G C G A G L4440 t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

180 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t

240 c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g

300 rrnB T2 terminator t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t

BsaHI 360 g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g

AclI 420 rrnB T1 terminator a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t

480 rrnB T1 terminator c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c

HincIIHpaI 540 M13 fwd C C C A G T C A C G A C G T T G T A A M13/pUC Forward T G T A A M13 Forward g c a g t t c c c t a c t c t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a c g t c a a g g g a t g a g a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t

AhdIAflIIBsoBIAvaIPspOMIApaI 600 M13 fwd attL3 A A C G M13/pUC Forward A A C G A C G G C C A G T M13 Forward a a c g a c g g c c a g t c t t a a g c t c g g g c c c c a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c t t g c t g c c g g t c a g a a t t c g a g c c c g g g g t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g

660 attL3 MPTLYNK c t g t t c g t t g c a a c a a a t t g a t g a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a t a a t a a g a c a a g c a a c g t t g t t t a a c t a c t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t a t t a t t

BamHIBspEI* 720 1 5 10 ATNFSLLKQAGDVE attL3 P2A VGGGSGATNFSLLKQAGDVE a g t t g g t g g c g g a t c c g g a g c t a c t a a t t t c t c t c t g t t a a a g c a g g c a g g a g a t g t g g a t c a a c c a c c g c c t a g g c c t c g a t g a t t a a a g a g a g a c a a t t t c g t c c g t c c t c t a c a c c t

MscIBseRIBsu36I 780 15 ENPGP KG 1 5 10 MAKPLSQEESTLI P2A BSD ENPGPKGMAKPLSQEESTLI g g a g a a t c c a g g t c c t a a g g g a a t g g c c a a g c c t t t g t c t c a a g a a g a a t c c a c c c t c a t c c t c t t a g g t c c a g g a t t c c c t t a c c g g t t c g g a a a c a g a g t t c t t c t t a g g t g g g a g t a

840 15 20 25 30 ERATATINSIPISEDYSVAS BSD ERATATINSIPISEDYSVAS t g a a a g a g c a a c g g c t a c a a t c a a c a g c a t c c c c a t c t c t g a a g a c t a c a g c g t c g c c a g a c t t t c t c g t t g c c g a t g t t a g t t g t c g t a g g g g t a g a g a c t t c t g a t g t c g c a g c g g t c

900 35 40 45 50 AALSSDGRIFTGVNVYHFTG BSD AALSSDGRIFTGVNVYHFTG c g c a g c t c t c t c t a g c g a c g g c c g c a t c t t c a c t g g t g t c a a t g t a t a t c a t t t t a c t g g g c g t c g a g a g a g a t c g c t g c c g g c g t a g a a g t g a c c a c a g t t a c a t a t a g t a a a a t g a c c

BsgI 960 55 60 65 70 GPCAELVVLGTAAAAAAGNL BSD GPCAELVVLGTAAAAAAGNL g g g a c c t t g t g c a g a a c t c g t g g t g c t g g g c a c t g c t g c t g c t g c g g c a g c t g g c a a c c t c c c t g g a a c a c g t c t t g a g c a c c a c g a c c c g t g a c g a c g a c g a c g c c g t c g a c c g t t g g a

1020 75 80 85 90 TCIVAIGNENRGILSPCGRC BSD TCIVAIGNENRGILSPCGRC g a c t t g t a t c g t c g c g a t c g g a a a t g a g a a c a g g g g c a t c t t g a g c c c c t g c g g a c g g t g c t g a a c a t a g c a g c g c t a g c c t t t a c t c t t g t c c c c g t a g a a c t c g g g g a c g c c t g c c a c

BsaBI* 1080 95 100 105 110 RQVLLDLHPGIKAIVKDSDG BSD RQVLLDLHPGIKAIVKDSDG c c g a c a g g t g c t t c t c g a t c t g c a t c c t g g g a t c a a a g c c a t a g t g a a g g a c a g t g a t g g g g c t g t c c a c g a a g a g c t a g a c g t a g g a c c c t a g t t t c g g t a t c a c t t c c t g t c a c t a c c

1140 115 120 125 130 QPTAVGIRELLPSGYVWEG* BSD QPTAVGIRELLPSGYVWEG* a c a g c c g a c g g c a g t t g g g a t t c g t g a a t t g c t g c c c t c t g g t t a t g t g t g g g a g g g c t a t g t c g g c t g c c g t c a a c c c t a a g c a c t t a a c g a c g g g a g a c c a a t a c a c a c c c t c c c g a t

BclI*Eco53kISacIEcoRIHindIIIBlpI 1200 BSD bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a g a a t t c t g a a g c t t a g c c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c t c t t a a g a c t t c g a a t c g g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g

1260 bGH poly(A) signal a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t

BtgZI 1320 bGH poly(A) signal c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t

1380 bGH poly(A) signal t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a g a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t c t t a t c g t c c g

BsrGITatISphIAcc65IKpnI 1440 bGH poly(A) signal attL2 a t g c t g g g g a g g t a c c t g a c c c a g c t t t c t t g t a c a a a g t t g g c a t t a t a a g a a a g c a t t t a c g a c c c c t c c a t g g a c t g g g t c g a a a g a a c a t g t t t c a a c c g t a a t a t t c t t t c g t a a

1500 attL2 g c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c c c g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g g

EcoRVNotIPstI 1560 T7 promoter M13 rev G G G A T A T C A C T C A G C A T A A T T7 C A G T A T C G A M13 Reverse a t c c a g c t g c a g g g c g g c c g c g a t a t c c c c t a t a g t g a g t c g t a t t a c a t g g t c a t a g c t t a g g t c g a c g t c c c g c c g g c g c t a t a g g g g a t a t c a c t c a g c a t a a t g t a c c a g t a t c g a

1620 M13 rev C A A A G G A C M13 Reverse g t t t c c t g g c a g c t c t g g c c c g t g t c t c a a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a c a a a g g a c c g t c g a g a c c g g g c a c a g a g t t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t

1680 G T T C C C C A C A A pENTR-R a a t a t a t c a t c a t g a a c a a t a a a a c t g t c t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t t t a t a t a g t a g t a c t t g t t a t t t t g a c a g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a

1740 1 5 10 15 20 MSHIQRETSRPRLNSNMDAD KanR T A C T C G G T A pENTR-R MSHIQRETSRPRLNSNMDAD a t g a g c c a t a t t c a a c g g g a a a c g t c g a g g c c g c g a t t a a a t t c c a a c a t g g a t g c t g a t t a c t c g g t a t a a g t t g c c c t t t g c a g c t c c g g c g c t a a t t t a a g g t t g t a c c t a c g a c t a

1800 25 30 35 40 LYGYKWARDNVGQSGATIYR KanR C C A T A T T T A C C C G A G C G C T A Kan-R LYGYKWARDNVGQSGATIYR t t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t c t a t c g c a a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a g a t a g c g

1860 45 50 55 60 LYGKPDAPELFLKHGKGSVA KanR LYGKPDAPELFLKHGKGSVA t t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g c g t t g c c a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c g c a a c g g

1920 65 70 75 80 NDVTDEMVRLNWLTEFMPLP KanR NDVTDEMVRLNWLTEFMPLP a a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c t c t t c c g t t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g a g a a g g c

1980 85 90 95 100 TIKHFIRTPDDAWLLTTAIP KanR TIKHFIRTPDDAWLLTTAIP a c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c g a t c c c c t g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g c t a g g g g

SspIEcoNI 2040 105 110 115 120 GKTAFQVLEEYPDSGENIVD KanR GKTAFQVLEEYPDSGENIVD g g a a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t t g t t g a t c c t t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a a c a a c t a

BsrFI 2100 125 130 135 140 ALAVFLRRLHSIPVCNCPFN KanR ALAVFLRRLHSIPVCNCPFN g c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c t t t t a a c c g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g a a a a t t g

BsmBIEsp3IBpu10IAsiSI 2160 145 150 155 160 SDRVFRLAQAQSRMNNGLVD KanR SDRVFRLAQAQSRMNNGLVD a g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t g g t t g a t t c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a c c a a c t a

2220 165 170 175 180 ASDFDDERNGWPVEQVWKEM KanR ASDFDDERNGWPVEQVWKEM g c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a a g a a a t g c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t t c t t t a c

2280 185 190 195 200 HKLLPFSPDSVVTHGDFSLD KanR HKLLPFSPDSVVTHGDFSLD c a t a a a c t t t t g c c a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c a c t t g a t g t a t t t g a a a a c g g t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g t g a a c t a

2340 205 210 215 220 NLIFDEGKLIGCIDVGRVGI KanR NLIFDEGKLIGCIDVGRVGI a a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t c g g a a t c t t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a g c c t t a g

PflMI 2400 225 230 235 240 ADRYQDLAILWNCLGEFSPS KanR ADRYQDLAILWNCLGEFSPS g c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c t c c t t c a c g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g a g g a a g t

2460 245 250 255 260 LQKRLFQKYGIDNPDMNKLQ KanR LQKRLFQKYGIDNPDMNKLQ t t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a t t g c a g a a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t t a a c g t c

2520 265 270 FHLMLDEFF* KanR FHLMLDEFF* t t t c a t t t g a t g c t c g a t g a g t t t t t c t a a t c a g a a t t g g t t a a t t g g t t g t a a c a c t g g a a a g t a a a c t a c g a g c t a c t c a a a a a g a t t a g t c t t a a c c a a t t a a c c a a c a t t g t g a c c

2580 c a g a g c a t t a c g c t g a c t t g a c g g g a c g g c g c a a g c t c a t g a c c a a a a t c c c t t a a c g t g g t c t c g t a a t g c g a c t g a a c t g c c c t g c c g c g t t c g a g t a c t g g t t t t a g g g a a t t g c a c

2640 ori a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t

2700 ori g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c

2760 ori g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g

2820 ori a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c

AlwNI 2880 ori a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g

2940 ori a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c

ApaLI 3000 ori c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g

3060 ori a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t

BciVI 3120 ori a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a

3180 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c

3240 ori c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c

PciI 3287 g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.