Addgene pC0.018 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pC0.018 Sequencing Result


Map View

SacI (6194) Eco53kI (6192) HincII (6178) BamHI (5612) AclI (4840) MluI (4806) NruI (4565) PmlI (4527) PvuII (4319) KpnI (4012) Acc65I - BanI (4008) BsmI (3838) ScaI (3754) NcoI (3657) EcoRI (3574) BstBI (3572) SphI (3495) AleI (3481) BmtI (3337) PflMI (0) EcoO109I (22) pBRforEco (24 .. 42) ZraI (81) AatII (83) AvaI - BsoBI (326) BstEII (594) PvuI (815) BtgZI (992) BglI (1066) NgoMIV (1083) NaeI (1085) BssHII (1158) SnaBI (1191) AlwNI (1633) BseYI (1738) PspFI (1742) pBR322ori-F (1887 .. 1906) L4440 (2140 .. 2157) BstZ17I (2449) BmgBI (2454) SwaI (2755) BsgI (2857) BstAPI (3015) NheI (3333) pC0.018 6402 bp

Sequence View

EcoO109IPflMI 5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g t g g c t c t t c a g t g g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t c a c c g a g a a g t c a c c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a

ZraIAatII 120 AmpR promoter g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g

180 AmpR promoter t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c

240 AmpR promoter a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g c g c t c a c g c a a c t g g t c c a g a a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c g c g a g t g c g t t g a c c a g g t c t t g

300 c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c

BsoBIAvaI 360 MPRASKQQARYAV t t t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g a a a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c

420 GRCLMLWSSNDVTQQGSRPK g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t

480 1 5 10 15 MREAVIAEVSTQLSE SmR TKLNIMREAVIAEVSTQLSE a c a a a g t t a a a c a t c a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g t g t t t c a a t t t g t a g t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c

540 20 25 30 35 VVGVIERHLEPTLLAVHLYG SmR VVGVIERHLEPTLLAVHLYG g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g

BstEII 600 40 45 50 55 SAVDGGLKPHSDIDLLVTVT SmR SAVDGGLKPHSDIDLLVTVT t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g c g a t a t t g a t t t g c t g g t t a c g g t g a c c a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c g c t a t a a c t a a a c g a c c a a t g c c a c t g g

660 60 65 70 75 VRLDETTRRALINDLLETSA SmR VRLDETTRRALINDLLETSA g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a

720 80 85 90 95 SPGESEILRAVEVTIVVHDD SmR SPGESEILRAVEVTIVVHDD t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g

780 100 105 110 115 IIPWRYPAKRELQFGEWQRN SmR IIPWRYPAKRELQFGEWQRN a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a

PvuI 840 120 125 130 135 DILAGIFEPATIDIDLAILL SmR DILAGIFEPATIDIDLAILL g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c

900 140 145 150 155 TKAREHSVALVGPAAEELFD SmR TKAREHSVALVGPAAEELFD a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a

960 160 165 170 175 PVPEQDLFEALNETLTLWNS SmR PVPEQDLFEALNETLTLWNS c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c

BtgZI 1020 180 185 190 195 PPDWAGDERNVVLTLSRIWY SmR PPDWAGDERNVVLTLSRIWY c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g

BglI 1080 200 205 210 215 SAVTGKIAPKDVAADWAMER SmR SAVTGKIAPKDVAADWAMER a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g

NgoMIVNaeI 1140 220 225 230 235 LPAQYQPVILEARQAYLGQE SmR LPAQYQPVILEARQAYLGQE c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t

SnaBIBssHII 1200 240 245 250 255 EDRLASRADQLEEFVHYVKG SmR EDRLASRADQLEEFVHYVKG g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a t t a c g t a a a a g g c c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t a a t g c a t t t t c c g

1260 260 EITKVVGK* SmR EITKVVGK* g a g a t c a c c a a g g t a g t c g g c a a a t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g c t c t a g t g g t t c c a t c a g c c g t t t a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c

1320 a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a

1380 c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t

1440 ori a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t

1500 ori a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a

1560 ori c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c

1620 ori t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g

AlwNI 1680 ori c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t

BseYI 1740 ori c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g

PspFI 1800 ori a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g

1860 ori g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t

1920 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c

1980 ori t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t

2040 ori t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a

2100 c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c

2160 A G C G A G T C A G T G A G C G A G L4440 t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t

2220 g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t c a c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a g t

2280 1 5 MDKKYSIGL 10 A 15 IGTNS dCas9 MDKKYSIGLAIGTNS c t c t g t g g t c t c a a a t g g a t a a g a a a t a c t c a a t a g g c t t a g c t a t c g g c a c a a a t a g c g g a g a c a c c a g a g t t t a c c t a t t c t t t a t g a g t t a t c c g a a t c g a t a g c c g t g t t t a t c g c

2340 20 25 30 35 VGWAVITDEYKVPSKKFKVL dCas9 VGWAVITDEYKVPSKKFKVL t c g g a t g g g c g g t g a t c a c t g a t g a a t a t a a g g t t c c g t c t a a a a a g t t c a a g g t t c t g g a g c c t a c c c g c c a c t a g t g a c t a c t t a t a t t c c a a g g c a g a t t t t t c a a g t t c c a a g a c c

2400 40 45 50 55 GNTDRHSIKKNLIGALLFDS dCas9 GNTDRHSIKKNLIGALLFDS g a a a t a c a g a c c g c c a c a g t a t c a a a a a a a a t c t t a t a g g g g c t c t t t t a t t t g a c a g t g c t t t a t g t c t g g c g g t g t c a t a g t t t t t t t t a g a a t a t c c c c g a g a a a a t a a a c t g t c a c

BstZ17IBmgBI 2460 60 65 70 75 GETAEATRLKRTARRRYTRR dCas9 GETAEATRLKRTARRRYTRR g a g a g a c a g c g g a a g c g a c t c g t c t c a a a c g g a c a g c t c g t a g a a g g t a t a c a c g t c g g a c t c t c t g t c g c c t t c g c t g a g c a g a g t t t g c c t g t c g a g c a t c t t c c a t a t g t g c a g c c t

2520 80 85 90 95 KNRICYLQEIFSNEMAKVDD dCas9 KNRICYLQEIFSNEMAKVDD a g a a t c g t a t t t g t t a t c t a c a g g a g a t t t t t t c a a a t g a g a t g g c g a a a g t a g a t g a t a t c t t a g c a t a a a c a a t a g a t g t c c t c t a a a a a a g t t t a c t c t a c c g c t t t c a t c t a c t a t

2580 100 105 110 115 SFFHRLEESFLVEEDKKHER dCas9 SFFHRLEESFLVEEDKKHER g t t t c t t t c a t c g a c t t g a a g a g t c t t t t t t g g t g g a a g a a g a t a a g a a g c a t g a a c g t c c a a a g a a a g t a g c t g a a c t t c t c a g a a a a a a c c a c c t t c t t c t a t t c t t c g t a c t t g c a g

2640 120 125 130 135 HPIFGNIVDEVAYHEKYPTI dCas9 HPIFGNIVDEVAYHEKYPTI a t c c t a t t t t t g g a a a t a t a g t a g a t g a a g t t g c t t a t c a t g a g a a a t a t c c a a c t a t c t t a g g a t a a a a a c c t t t a t a t c a t c t a c t t c a a c g a a t a g t a c t c t t t a t a g g t t g a t a g a

2700 140 145 150 155 YHLRKKLVDSTDKADLRLIY dCas9 YHLRKKLVDSTDKADLRLIY a t c a t c t g c g a a a a a a a t t g g t a g a t t c t a c t g a t a a a g c g g a t t t g c g c t t a a t c t a t t t a g t a g a c g c t t t t t t t a a c c a t c t a a g a t g a c t a t t t c g c c t a a a c g c g a a t t a g a t a a

SwaI 2760 160 165 170 175 LALAHMIKFRGHFLIEGDLN dCas9 LALAHMIKFRGHFLIEGDLN t g g c c t t a g c g c a t a t g a t t a a g t t t c g t g g t c a t t t t t t g a t t g a g g g a g a t t t a a a t c a c c g g a a t c g c g t a t a c t a a t t c a a a g c a c c a g t a a a a a a c t a a c t c c c t c t a a a t t t a g

2820 180 185 190 195 PDNSDVDKLFIQLVQTYNQL dCas9 PDNSDVDKLFIQLVQTYNQL c t g a t a a t a g t g a t g t g g a c a a a c t a t t t a t c c a g t t g g t a c a a a c c t a c a a t c a a t t a t g a c t a t t a t c a c t a c a c c t g t t t g a t a a a t a g g t c a a c c a t g t t t g g a t g t t a g t t a a t a

BsgI 2880 200 205 210 215 FEENPINASGVDAKAILSAR dCas9 FEENPINASGVDAKAILSAR t t g a a g a a a a c c c t a t t a a c g c a a g t g g a g t a g a t g c t a a a g c g a t t c t t t c t g c a c g a t a a c t t c t t t t g g g a t a a t t g c g t t c a c c t c a t c t a c g a t t t c g c t a a g a a a g a c g t g c t a

2940 220 225 230 235 LSKSRRLENLIAQLPGEKKN dCas9 LSKSRRLENLIAQLPGEKKN t g a g t a a a t c a a g a c g a t t a g a a a a t c t c a t t g c t c a g c t c c c c g g t g a g a a g a a a a a t g a c t c a t t t a g t t c t g c t a a t c t t t t a g a g t a a c g a g t c g a g g g g c c a c t c t t c t t t t t a c

3000 240 245 250 255 GLFGNLIALSLGLTPNFKSN dCas9 GLFGNLIALSLGLTPNFKSN g c t t a t t t g g g a a t c t c a t t g c t t t g t c a t t g g g t t t g a c c c c t a a t t t t a a a t c a a a t t c g a a t a a a c c c t t a g a g t a a c g a a a c a g t a a c c c a a a c t g g g g a t t a a a a t t t a g t t t a a

BstAPI 3060 260 265 270 275 FDLAEDAKLQLSKDTYDDDL dCas9 FDLAEDAKLQLSKDTYDDDL t t g a t t t g g c a g a a g a t g c t a a a t t a c a g c t t t c a a a a g a t a c t t a c g a t g a t g a t t t a g a a c t a a a c c g t c t t c t a c g a t t t a a t g t c g a a a g t t t t c t a t g a a t g c t a c t a c t a a a t c

3120 280 285 290 295 DNLLAQIGDQYADLFLAAKN dCas9 DNLLAQIGDQYADLFLAAKN a t a a t t t a t t g g c g c a a a t t g g a g a t c a a t a t g c t g a t t t g t t t t t g g c a g c t a a g a a t t t a t t a a a t a a c c g c g t t t a a c c t c t a g t t a t a c g a c t a a a c a a a a a c c g t c g a t t c t t a a

3180 300 305 310 315 LSDAILLSDILRVNTEITKA dCas9 LSDAILLSDILRVNTEITKA t a t c a g a t g c t a t t t t a c t t t c a g a t a t c c t a a g a g t a a a t a c t g a a a t a a c t a a g g c t c a t a g t c t a c g a t a a a a t g a a a g t c t a t a g g a t t c t c a t t t a t g a c t t t a t t g a t t c c g a g

3240 320 325 330 335 PLSASMIKRYDEHHQDLTLL dCas9 PLSASMIKRYDEHHQDLTLL c c c t a t c a g c t t c a a t g a t t a a a c g c t a c g a t g a a c a t c a t c a a g a c t t g a c t c t t t t a a g g g a t a g t c g a a g t t a c t a a t t t g c g a t g c t a c t t g t a g t a g t t c t g a a c t g a g a a a a t t

3300 340 345 350 355 KALVRQQLPEKYKEIFFDQS dCas9 KALVRQQLPEKYKEIFFDQS a a g c t t t a g t t c g a c a a c a a c t t c c a g a a a a g t a t a a a g a a a t c t t t t t t g a t c a a t c a a t t c g a a a t c a a g c t g t t g t t g a a g g t c t t t t c a t a t t t c t t t a g a a a a a a c t a g t t a g t t

BmtINheI 3360 360 365 370 375 KNGYAGYIDGGASQEEFYKF dCas9 KNGYAGYIDGGASQEEFYKF a a a a c g g a t a t g c a g g t t a t a t t g a t g g g g g a g c t a g c c a a g a a g a a t t t t a t a a a t t t a t t t t g c c t a t a c g t c c a a t a t a a c t a c c c c c t c g a t c g g t t c t t c t t a a a a t a t t t a a a t

3420 380 385 390 395 IKPILEKMDGTEELLVKLNR dCas9 IKPILEKMDGTEELLVKLNR t c a a a c c a a t t t t a g a a a a a a t g g a t g g t a c t g a g g a a t t a t t g g t g a a a c t a a a t c g t g a g t t t g g t t a a a a t c t t t t t t a c c t a c c a t g a c t c c t t a a t a a c c a c t t t g a t t t a g c a c

3480 400 405 410 415 EDLLRKQRTFDNGSIPHQIH dCas9 EDLLRKQRTFDNGSIPHQIH a a g a t t t g c t g c g c a a g c a a c g g a c c t t t g a c a a c g g c t c t a t t c c c c a t c a a a t t c a c t t t c t a a a c g a c g c g t t c g t t g c c t g g a a a c t g t t g c c g a g a t a a g g g g t a g t t t a a g t g a

SphIAleI 3540 420 425 430 435 LGELHAILRRQEDFYPFLKD dCas9 LGELHAILRRQEDFYPFLKD t g g g t g a g c t g c a t g c t a t t t t g a g a c g a c a a g a a g a t t t t t a t c c a t t t t t a a a a g a c a a c c c a c t c g a c g t a c g a t a a a a c t c t g c t g t t c t t c t a a a a a t a g g t a a a a a t t t t c t g t

BstBIEcoRI 3600 440 445 450 455 NREKIEKILTFRIPYYVGPL dCas9 NREKIEKILTFRIPYYVGPL a t c g t g a g a a g a t t g a a a a a a t c t t g a c t t t t c g a a t t c c t t a t t a t g t t g g t c c a t t g g t a g c a c t c t t c t a a c t t t t t t a g a a c t g a a a a g c t t a a g g a a t a a t a c a a c c a g g t a a c c

NcoI 3660 460 465 470 475 ARGNSRFAWMTRKSEETITP dCas9 ARGNSRFAWMTRKSEETITP c g c g t g g c a a t a g t c g t t t t g c a t g g a t g a c t c g g a a g t c t g a a g a a a c a a t t a c c c c a t g c g c a c c g t t a t c a g c a a a a c g t a c c t a c t g a g c c t t c a g a c t t c t t t g t t a a t g g g g t a

3720 480 485 490 495 WNFEEVVDKGASAQSFIERM dCas9 WNFEEVVDKGASAQSFIERM g g a a t t t t g a a g a a g t t g t c g a t a a a g g t g c t t c a g c t c a a t c a t t t a t t g a a c g c a t g a c c t t a a a a c t t c t t c a a c a g c t a t t t c c a c g a a g t c g a g t t a g t a a a t a a c t t g c g t a c t

ScaI 3780 500 505 510 515 TNFDKNLPNEKVLPKHSLLY dCas9 TNFDKNLPNEKVLPKHSLLY c a a a c t t t g a t a a a a a t c t t c c a a a t g a a a a a g t a c t a c c a a a a c a t a g t t t g c t t t a t g g t t t g a a a c t a t t t t t a g a a g g t t t a c t t t t t c a t g a t g g t t t t g t a t c a a a c g a a a t a c

BsmI 3840 520 525 530 535 EYFTVYNELTKVKYVTEGMR dCas9 EYFTVYNELTKVKYVTEGMR a g t a t t t t a c g g t t t a t a a c g a a t t g a c a a a g g t c a a a t a t g t t a c t g a a g g a a t g c g a a t c a t a a a a t g c c a a a t a t t g c t t a a c t g t t t c c a g t t t a t a c a a t g a c t t c c t t a c g c t t

3900 540 545 550 555 KPAFLSGEQKKAIVDLLFKT dCas9 KPAFLSGEQKKAIVDLLFKT a a c c a g c a t t t c t t t c a g g t g a a c a g a a g a a a g c c a t t g t t g a t t t a c t c t t c a a a a c a a t t g g t c g t a a a g a a a g t c c a c t t g t c t t c t t t c g g t a a c a a c t a a a t g a g a a g t t t t g t t

3960 560 565 570 575 NRKVTVKQLKEDYFKKIECF dCas9 NRKVTVKQLKEDYFKKIECF a t c g a a a a g t a a c c g t t a a g c a a t t a a a a g a a g a t t a t t t c a a a a a a a t a g a a t g t t t t g t a g c t t t t c a t t g g c a a t t c g t t a a t t t t c t t c t a a t a a a g t t t t t t t a t c t t a c a a a a c

Acc65IBanIKpnI 4020 580 585 590 595 DSVEISGVEDRFNASLGTYH dCas9 DSVEISGVEDRFNASLGTYH a t a g t g t t g a a a t t t c a g g a g t t g a a g a t a g a t t t a a t g c t t c a t t a g g t a c c t a c c a t g t a t c a c a a c t t t a a a g t c c t c a a c t t c t a t c t a a a t t a c g a a g t a a t c c a t g g a t g g t a c

4080 600 605 610 615 DLLKIIKDKDFLDNEENEDI dCas9 DLLKIIKDKDFLDNEENEDI a t t t g c t a a a a a t t a t t a a a g a t a a a g a t t t t t t g g a t a a t g a a g a a a a t g a a g a t a t c t t a a a c g a t t t t t a a t a a t t t c t a t t t c t a a a a a a c c t a t t a c t t c t t t t a c t t c t a t a g a

4140 620 625 630 635 LEDIVLTLTLFEDREMIEER dCas9 LEDIVLTLTLFEDREMIEER t a g a g g a t a t t g t t t t a a c a t t g a c c t t a t t t g a a g a t a g g g a g a t g a t t g a g g a a a g a c a t c t c c t a t a a c a a a a t t g t a a c t g g a a t a a a c t t c t a t c c c t c t a c t a a c t c c t t t c t g

4200 640 645 650 655 LKTYAHLFDDKVMKQLKRRR dCas9 LKTYAHLFDDKVMKQLKRRR t t a a a a c a t a t g c t c a c c t c t t t g a t g a t a a g g t g a t g a a a c a g c t t a a a c g t c g c c g t t a a t t t t g t a t a c g a g t g g a g a a a c t a c t a t t c c a c t a c t t t g t c g a a t t t g c a g c g g c a a

4260 660 665 670 675 YTGWGRLSRKLINGIRDKQS dCas9 YTGWGRLSRKLINGIRDKQS a t a c t g g t t g g g g a c g t t t g t c t c g a a a a t t g a t t a a t g g t a t t a g g g a t a a g c a a t c t g t a t g a c c a a c c c c t g c a a a c a g a g c t t t t a a c t a a t t a c c a t a a t c c c t a t t c g t t a g a c

PvuII 4320 680 685 690 695 GKTILDFLKSDGFANRNFMQ dCas9 GKTILDFLKSDGFANRNFMQ g c a a a a c a a t a t t a g a t t t t t t g a a a t c a g a t g g t t t t g c c a a t c g c a a t t t t a t g c a g c c g t t t t g t t a t a a t c t a a a a a a c t t t a g t c t a c c a a a a c g g t t a g c g t t a a a a t a c g t c g

4380 700 705 710 715 LIHDDSLTFKEDIQKAQVSG dCas9 LIHDDSLTFKEDIQKAQVSG t g a t c c a t g a t g a t a g t t t g a c a t t t a a a g a a g a t a t t c a a a a a g c a c a a g t g t c t g g a c a c t a g g t a c t a c t a t c a a a c t g t a a a t t t c t t c t a t a a g t t t t t c g t g t t c a c a g a c c t g

4440 720 725 730 735 QGDSLHEHIANLAGSPAIKK dCas9 QGDSLHEHIANLAGSPAIKK a a g g c g a t a g t t t a c a t g a a c a t a t t g c a a a t t t a g c t g g t a g c c c t g c t a t t a a a a a a g t t c c g c t a t c a a a t g t a c t t g t a t a a c g t t t a a a t c g a c c a t c g g g a c g a t a a t t t t t t c

4500 740 745 750 755 GILQTVKVVDELVKVMGRHK dCas9 GILQTVKVVDELVKVMGRHK g t a t t t t a c a g a c t g t a a a a g t t g t t g a t g a a t t g g t c a a a g t a a t g g g g c g g c a t a a g c c a t a a a a t g t c t g a c a t t t t c a a c a a c t a c t t a a c c a g t t t c a t t a c c c c g c c g t a t t c g

PmlI 4560 760 765 770 775 PENIVIEMARENQTTQKGQK dCas9 PENIVIEMARENQTTQKGQK c a g a a a a t a t c g t t a t t g a a a t g g c a c g t g a a a a t c a g a c a a c t c a a a a g g g c c a g a a a a g t c t t t t a t a g c a a t a a c t t t a c c g t g c a c t t t t a g t c t g t t g a g t t t t c c c g g t c t t t t

NruI 4620 780 785 790 795 NSRERMKRIEEGIKELGSQI dCas9 NSRERMKRIEEGIKELGSQI a t t c g c g a g a g c g t a t g a a a c g a a t c g a a g a a g g t a t c a a a g a a t t a g g a a g t c a g a t t c t a a g c g c t c t c g c a t a c t t t g c t t a g c t t c t t c c a t a g t t t c t t a a t c c t t c a g t c t a a g

4680 800 805 810 815 LKEHPVENTQLQNEKLYLYY dCas9 LKEHPVENTQLQNEKLYLYY t t a a a g a g c a t c c t g t t g a a a a t a c t c a a t t g c a a a a t g a a a a g c t c t a t c t c t a t t a t c a a t t t c t c g t a g g a c a a c t t t t a t g a g t t a a c g t t t t a c t t t t c g a g a t a g a g a t a a t a g

4740 820 825 830 835 LQNGRDMYVDQELDINRLSD dCas9 LQNGRDMYVDQELDINRLSD t c c a a a a t g g a a g a g a c a t g t a t g t g g a c c a a g a a t t a g a t a t t a a t c g t t t a a g t g a t t a g g t t t t a c c t t c t c t g t a c a t a c a c c t g g t t c t t a a t c t a t a a t t a g c a a a t t c a c t a a

4800 YDVD 840 A 845 850 855 IVPQSFLKDDSIDNK dCas9 YDVDAIVPQSFLKDDSIDNK a t g a t g t c g a t g c c a t t g t t c c a c a a a g t t t c c t t a a a g a c g a t t c a a t a g a c a a t a a g g t a c t a c a g c t a c g g t a a c a a g g t g t t t c a a a g g a a t t t c t g c t a a g t t a t c t g t t a t t c c

AclIMluI 4860 860 865 870 875 VLTRSDKNRGKSDNVPSEEV dCas9 VLTRSDKNRGKSDNVPSEEV t c t t a a c g c g t t c t g a t a a a a a t c g t g g t a a a t c g g a t a a c g t t c c a a g t g a a g a a g t a g a g a a t t g c g c a a g a c t a t t t t t a g c a c c a t t t a g c c t a t t g c a a g g t t c a c t t c t t c a t c

4920 880 885 890 895 VKKMKNYWRQLLNAKLITQR dCas9 VKKMKNYWRQLLNAKLITQR t c a a a a a g a t g a a a a a c t a t t g g a g a c a a c t t c t a a a c g c c a a g t t a a t c a c t c a a c g t a a g t t t t t c t a c t t t t t g a t a a c c t c t g t t g a a g a t t t g c g g t t c a a t t a g t g a g t t g c a t

4980 900 905 910 915 KFDNLTKAERGGLSELDKAG dCas9 KFDNLTKAERGGLSELDKAG a g t t t g a t a a t t t a a c g a a a g c t g a a c g t g g a g g t t t g a g t g a a c t t g a t a a a g c t g g t t t c a a a c t a t t a a a t t g c t t t c g a c t t g c a c c t c c a a a c t c a c t t g a a c t a t t t c g a c c a a

5040 920 925 930 935 FIKRQLVETRQITKHVAQIL dCas9 FIKRQLVETRQITKHVAQIL t t a t c a a a c g c c a a t t g g t t g a a a c t c g c c a a a t c a c t a a g c a t g t g g c a c a a a t t t t g g a a t a g t t t g c g g t t a a c c a a c t t t g a g c g g t t t a g t g a t t c g t a c a c c g t g t t t a a a a c c

5100 940 945 950 955 DSRMNTKYDENDKLIREVKV dCas9 DSRMNTKYDENDKLIREVKV a t a g t c g c a t g a a t a c t a a a t a c g a t g a a a a t g a t a a a c t t a t t c g a g a g g t t a a a g t g a t a t c a g c g t a c t t a t g a t t t a t g c t a c t t t t a c t a t t t g a a t a a g c t c t c c a a t t t c a c t

5160 960 965 970 975 ITLKSKLVSDFRKDFQFYKV dCas9 ITLKSKLVSDFRKDFQFYKV t t a c c t t a a a a t c t a a a t t a g t t t c t g a c t t c c g a a a a g a t t t c c a a t t c t a t a a a g t a c a a t g g a a t t t t a g a t t t a a t c a a a g a c t g a a g g c t t t t c t a a a g g t t a a g a t a t t t c a t g

5220 980 985 990 995 REINNYHHAHDAYLNAVVGT dCas9 REINNYHHAHDAYLNAVVGT g t g a g a t t a a c a a t t a c c a t c a t g c c c a t g a t g c g t a t c t a a a t g c c g t c g t t g g a a c t g c a c t c t a a t t g t t a a t g g t a g t a c g g g t a c t a c g c a t a g a t t t a c g g c a g c a a c c t t g a c

5280 1000 1005 1010 1015 ALIKKYPKLESEFVYGDYKV dCas9 ALIKKYPKLESEFVYGDYKV c t t t g a t t a a g a a a t a t c c a a a a c t t g a a t c g g a g t t t g t c t a t g g t g a t t a t a a a g t t t g a a a c t a a t t c t t t a t a g g t t t t g a a c t t a g c c t c a a a c a g a t a c c a c t a a t a t t t c a a a

5340 1020 1025 1030 1035 YDVRKMIAKSEQEIGKATAK dCas9 YDVRKMIAKSEQEIGKATAK a t g a t g t t c g t a a a a t g a t t g c t a a g t c t g a g c a a g a a a t a g g c a a a g c a a c c g c a a a a t t a c t a c a a g c a t t t t a c t a a c g a t t c a g a c t c g t t c t t t a t c c g t t t c g t t g g c g t t t t a

5400 1040 1045 1050 1055 YFFYSNIMNFFKTEITLANG dCas9 YFFYSNIMNFFKTEITLANG a t t t c t t t t a c t c t a a t a t c a t g a a c t t c t t c a a a a c a g a a a t t a c a c t t g c a a a t g g a g t a a a g a a a a t g a g a t t a t a g t a c t t g a a g a a g t t t t g t c t t t a a t g t g a a c g t t t a c c t c

5460 1060 1065 1070 1075 EIRKRPLIETNGETGEIVWD dCas9 EIRKRPLIETNGETGEIVWD a g a t t c g c a a a c g c c c t c t a a t c g a a a c t a a t g g g g a a a c t g g a g a a a t t g t c t g g g a t a t c t a a g c g t t t g c g g g a g a t t a g c t t t g a t t a c c c c t t t g a c c t c t t t a a c a g a c c c t a t

5520 1080 1085 1090 1095 KGRDFATVRKVLSMPQVNIV dCas9 KGRDFATVRKVLSMPQVNIV a a g g g c g a g a t t t t g c c a c a g t g c g c a a a g t a t t g t c c a t g c c c c a a g t c a a t a t t g t c a t t c c c g c t c t a a a a c g g t g t c a c g c g t t t c a t a a c a g g t a c g g g g t t c a g t t a t a a c a g t

5580 1100 1105 1110 1115 KKTEVQTGGFSKESILPKRN dCas9 KKTEVQTGGFSKESILPKRN a g a a a a c a g a a g t a c a g a c a g g c g g a t t c t c c a a g g a g t c a a t t t t a c c a a a a a g a a a t t t c t t t t g t c t t c a t g t c t g t c c g c c t a a g a g g t t c c t c a g t t a a a a t g g t t t t t c t t t a a

BamHI 5640 1120 1125 1130 1135 SDKLIARKKDWDPKKYGGFD dCas9 SDKLIARKKDWDPKKYGGFD c g g a c a a g c t t a t t g c t c g t a a a a a a g a c t g g g a t c c a a a a a a a t a t g g t g g t t t t g a t a g c c t g t t c g a a t a a c g a g c a t t t t t t c t g a c c c t a g g t t t t t t t a t a c c a c c a a a a c t a t

5700 1140 1145 1150 1155 SPTVAYSVLVVAKVEKGKSK dCas9 SPTVAYSVLVVAKVEKGKSK g t c c a a c g g t a g c t t a t t c a g t c c t a g t g g t t g c t a a g g t g g a a a a a g g g a a a t c g a a g a c a g g t t g c c a t c g a a t a a g t c a g g a t c a c c a a c g a t t c c a c c t t t t t c c c t t t a g c t t c t

5760 1160 1165 1170 1175 KLKSVKELLGITIMERSSFE dCas9 KLKSVKELLGITIMERSSFE a g t t a a a a t c c g t t a a a g a g t t a c t a g g g a t c a c a a t t a t g g a a a g a a g t t c c t t t g a a a t c a a t t t t a g g c a a t t t c t c a a t g a t c c c t a g t g t t a a t a c c t t t c t t c a a g g a a a c t t t

5820 1180 1185 1190 1195 KNPIDFLEAKGYKEVKKDLI dCas9 KNPIDFLEAKGYKEVKKDLI a a a a t c c g a t t g a c t t t t t a g a a g c t a a a g g a t a t a a g g a a g t t a a a a a a g a c t t a a t c a t t t t a g g c t a a c t g a a a a a t c t t c g a t t t c c t a t a t t c c t t c a a t t t t t t c t g a a t t a g t

5880 1200 1205 1210 1215 IKLPKYSLFELENGRKRMLA dCas9 IKLPKYSLFELENGRKRMLA t t a a a c t a c c t a a a t a t a g t c t t t t t g a g t t a g a a a a c g g t c g t a a a c g g a t g c t g g c t a a a t t t g a t g g a t t t a t a t c a g a a a a a c t c a a t c t t t t g c c a g c a t t t g c c t a c g a c c g a t

5940 1220 1225 1230 1235 SAGELQKGNELALPSKYVNF dCas9 SAGELQKGNELALPSKYVNF g t g c c g g a g a a t t a c a a a a a g g a a a t g a g c t g g c t c t g c c a a g c a a a t a t g t g a a t t t t t c a c g g c c t c t t a a t g t t t t t c c t t t a c t c g a c c g a g a c g g t t c g t t t a t a c a c t t a a a a a

6000 1240 1245 1250 1255 LYLASHYEKLKGSPEDNEQK dCas9 LYLASHYEKLKGSPEDNEQK t a t a t t t a g c t a g t c a t t a t g a a a a g t t g a a g g g t a g t c c a g a a g a t a a c g a a c a a a a a c a t a t a a a t c g a t c a g t a a t a c t t t t c a a c t t c c c a t c a g g t c t t c t a t t g c t t g t t t t t g

6060 1260 1265 1270 1275 QLFVEQHKHYLDEIIEQISE dCas9 QLFVEQHKHYLDEIIEQISE a a t t g t t t g t g g a g c a g c a t a a g c a t t a t t t a g a t g a g a t t a t t g a g c a a a t c a g t g a a t t t a a c a a a c a c c t c g t c g t a t t c g t a a t a a a t c t a c t c t a a t a a c t c g t t t a g t c a c t t a

6120 1280 1285 1290 1295 FSKRVILADANLDKVLSAYN dCas9 FSKRVILADANLDKVLSAYN t t t c t a a g c g t g t t a t t t t a g c a g a t g c c a a t t t a g a t a a a g t t c t t a g t g c a t a t a a c a a a a g a t t c g c a c a a t a a a a t c g t c t a c g g t t a a a t c t a t t t c a a g a a t c a c g t a t a t t g t

HincII 6180 1300 1305 1310 1315 KHRDKPIREQAENIIHLFTL dCas9 KHRDKPIREQAENIIHLFTL a a c a t a g a g a c a a a c c a a t a c g t g a a c a a g c a g a a a a t a t t a t t c a t t t a t t t a c g t t g a t t g t a t c t c t g t t t g g t t a t g c a c t t g t t c g t c t t t t a t a a t a a g t a a a t a a a t g c a a c t

Eco53kISacI 6240 1320 1325 1330 1335 TNLGAPAAFKYFDTTIDRKR dCas9 TNLGAPAAFKYFDTTIDRKR c g a a t c t t g g a g c t c c c g c t g c t t t t a a a t a t t t t g a t a c a a c a a t t g a t c g t a a a c g a t g c t t a g a a c c t c g a g g g c g a c g a a a a t t t a t a a a a c t a t g t t g t t a a c t a g c a t t t g c t a

6300 1340 1345 1350 1355 YTSTKEVLDATLIHQSITGL dCas9 YTSTKEVLDATLIHQSITGL a t a c g t c t a c a a a a g a a g t t t t a g a t g c c a c t c t t a t c c a t c a a t c c a t c a c t g g t c t t t t a t g c a g a t g t t t t c t t c a a a a t c t a c g g t g a g a a t a g g t a g t t a g g t a g t g a c c a g a a a

6360 1360 1365 YETRIDLSQLGGD (in frame with dCas9) AANNIVS dCas9 YETRIDLSQLGGDAANNIVS a t g a a a c a c g c a t t g a t t t g a g t c a g c t a g g a g g t g a c g c c g c c a a t a a c a t c g t t t c c t t a c t t t g t g c g t a a c t a a a c t c a g t c g a t c c t c c a c t g c g g c g g t t a t t g t a g c a a a g g a

3ʹ 5ʹ 6402 (in frame with dCas9) FKRVAIAA* FKRVAIAA* t t a a g c g g g t a g c c a t t g c c g c c t a a g c t t t g a g a c c a c g a a a a t t c g c c c a t c g g t a a c g g c g g a t t c g a a a c t c t g g t g c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.