Addgene pET21a-MTF-His - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pET21a-MTF-His


Map View

1000 750 500 250 End (1138) EcoO109I (1122) T7 Term (1092 .. 1110) BlpI (1095) MspA1I (1094) MflI * - BstYI (1042) BsiHKAI - Bsp1286I (1023) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (1016) BtgZI (965) BssHII (957) BstXI (780) MscI (772) EaeI (770) BtgI - NcoI (767) NmeAIII (708) AgeI - SgrAI - BsaWI (512) XcmI (490) MmeI (466) BglI (421) AclI (396) BpmI Eco57MI (389) PflMI (298) BbvCI - Bpu10I (237) BsiEI (188) BbsI (135) BsaAI - SnaBI (87) NdeI (70) XbaI (31) Start (0) RBS 6xHis pET21a-MTF-His 1138 bp

Sequence View

XbaIStart (0) 60 RBS t t t a g g g g a a t g k g a g c g a t a a c a a t t c c c t c t a g a a a t a a t t t g t t t a a c t t t a a g a a g a a a t c c c c t t a c m c t c g c t a t t g t t a a g g g a g a t c t t t a t t a a a c a a a t t g a a a t t c t t c

SnaBIBsaAINdeI 120 RBS g a g a t a t a c a t a t g t c a g a a t c a c t a c g t a t t a t t t t g c g g g t a c a c c t g a c t t t g c a g c c t c t a t a t g t a t a c a g t c t t a g t g a t g c a t a a t a a a a c g c c c a t g t g g a c t g a a a c g t c g

BbsI 180 g c g t c a t c t c g a c g c g c t g t t g t c t t c t g g t c a t a a c g t c g t t g g c g t g t t c a c c c a g c c c g c a g t a g a g c t g c g c g a c a a c a g a a g a c c a g t a t t g c a g c a a c c g c a c a a g t g g g t c g g

Bpu10IBbvCIBsiEI 240 MPSPVKVLAE a g a c c g a c c g g c a g g a c g c g g t a a a a a a c t g a t g c c c a g c c c g g t t a a a g t t c t g g c t g a t c t g g c t g g c c g t c c t g c g c c a t t t t t t g a c t a c g g g t c g g g c c a a t t t c a a g a c c g a c t

PflMI 300 EKGLPVFQPVSLRPQENQQL g g a a a a a g g t c t g c c c g t t t t t c a a c c t g t t t c c c t g c g t c c a c a a g a a a a c c a g c a a c t c c t t t t t c c a g a c g g g c a a a a a g t t g g a c a a a g g g a c g c a g g t g t t c t t t t g g t c g t t g a

360 VAELQADVMVVVAYGLILPK g g t c g c c g a a c t g c a g g c t g a t g t t a t g g t c g t c g t c g c c t a t g g t t t a a t t c t g c c g a a c c a g c g g c t t g a c g t c c g a c t a c a a t a c c a g c a g c a g c g g a t a c c a a a t t a a g a c g g c t t

AclIEco57MIBpmI 420 AVLEMPRLGCINVHGSLLPR a g c a g t g c t g g a g a t g c c g c g t c t t g g c t g t a t c a a c g t t c a t g g t t c a c t g c t g c c a c g t c g t c a c g a c c t c t a c g g c g c a g a a c c g a c a t a g t t g c a a g t a c c a a g t g a c g a c g g t g c

MmeIBglI 480 WRGAAPIQRSLWAGDAETGV c t g g c g c g g t g c t g c a c c a a t c c a a c g c t c a c t a t g g g c g g g t g a t g c a g a a a c t g g t g t g a c c g c g c c a c g a c g t g g t t a g g t t g c g a g t g a t a c c c g c c c a c t a c g t c t t t g a c c a c a

BsaWISgrAIAgeIXcmI 540 TIMQMDVGLDTGDMLYKLSC g a c c a t t a t g c a a a t g g a t g t c g g t t t a g a c a c c g g t g a t a t g c t c t a t a a g c t c t c c t g c t g g t a a t a c g t t t a c c t a c a g c c a a a t c t g t g g c c a c t a t a c g a g a t a t t c g a g a g g a c

600 PITAEDTSGTLYDKLAELGP c c c g a t t a c t g c a g a a g a t a c c a g t g g t a c g c t g t a c g a c a a g c t g g c a g a g c t t g g c c c g g g c t a a t g a c g t c t t c t a t g g t c a c c a t g c g a c a t g c t g t t c g a c c g t c t c g a a c c g g g

660 QGLITTLKQLADGTAKPEVQ a c a a g g g c t t a t c a c c a c g t t g a a a c a a c t g g c a g a c g g c a c g g c g a a a c c a g a a g t t c a t g t t c c c g a a t a g t g g t g c a a c t t t g t t g a c c g t c t g c c g t g c c g c t t t g g t c t t c a a g t

NmeAIII 720 DETLVTYAEKLSKEEARIDW g g a c g a a a c t c t t g t c a c t t a c g c c g a g a a g t t g a g t a a a g a a g a a g c g c g t a t t g a c t g c c t g c t t t g a g a a c a g t g a a t g c g g c t c t t c a a c t c a t t t c t t c t t c g c g c a t a a c t g a c

BstXINcoIBtgIEaeIMscI 780 SLSAAQLERCIRAFNPWPMS g t c a c t t t c g g c a g c a c a g c t t g a a c g c t g c a t t c g c g c t t t c a a t c c a t g g c c a a t g a g c a g t g a a a g c c g t c g t g t c g a a c t t g c g a c g t a a g c g c g a a a g t t a g g t a c c g g t t a c t c

840 WLEIEGQPVKVWKASVIDTA c t g g c t g g a a a t t g a a g g a c a g c c g g t t a a a g t c t g g a a a g c a t c g g t c a t t g a t a c g g c g a c c g a c c t t t a a c t t c c t g t c g g c c a a t t t c a g a c c t t t c g t a g c c a g t a a c t a t g c c g

900 TNAAPGTILEANKQGIQVAT a a c c a a c g c t g c a c c a g g a a c g a t c c t t g a a g c c a a c a a a c a a g g c a t t c a g g t t g c g a c t t g g t t g c g a c g t g g t c c t t g c t a g g a a c t t c g g t t g t t t g t t c c g t a a g t c c a a c g c t g

BssHII 960 GDGILNLLSLQPAGKKAMSA t g g t g a t g g c a t c c t g a a c c t g c t c t c g t t a c a a c c t g c g g g t a a g a a a g c g a t g a g c g c a c c a c t a c c g t a g g a c t t g g a c g a g a g c a a t g t t g g a c g c c c a t t c t t t c g c t a c t c g c g

PaeR7IBsoBIPspXIXhoISmlIAvaIBtgZI 1020 QDLLNSRREWFVPGNRLVLE g c a a g a c c t c c t g a a c t c t c g t c g g g a a t g g t t t g t t c c g g g c a a c c g t c t g g t c c t c g a c g t t c t g g a g g a c t t g a g a g c a g c c c t t a c c a a a c a a g g c c c g t t g g c a g a c c a g g a g c t

BstYIMflI*Bsp1286IBsiHKAI 1080 1 5 HHHHHH * 6xHis HHHHHH* g c a c c a c c a c c a c c a c c a c t g a g a t c c g g c t g c t a a c a a a g c c c g a a a g g a a g c t g a g t t c g t g g t g g t g g t g g t g g t g a c t c t a g g c c g a c g a t t g t t t c g g g c t t t c c t t c g a c t c a a

End (1138) EcoO109IMspA1IBlpI 1138 G G C G A C T C G T T A T T G A T C G T7 Term g g c t g c t g c c a c c g c t g a g c a a t a a c t a g c a t a a c c c c t t g g g g c c t c t a a a c g g g t c c c g a c g a c g g t g g c g a c t c g t t a t t g a t c g t a t t g g g g a a c c c c g g a g a t t t g c c c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.