Addgene pWS1859 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pWS1859


Map View

TaqII (2696) NspI (2664) AflIII - PciI (2660) DrdI (2558) pBR322ori-F (2505 .. 2524) BssSI - BssSαI (2487) BciVI (2462) HaeII (2420) PspFI (2360) BseYI (2356) ApaLI (2346) BsiEI (2326) AcuI (2118) BspHI (1940) BsaAI (1927) MslI (1904) CAT-R (1749 .. 1768) TsoI (1721) PvuII (1720) BspEI (1620) AclI (1531) PflMI * (1391) PasI (1385) MscI (1353) NdeI (11) BmgBI (72) XcmI (156) BglII (213) BsrGI (377) BbvCI (430) BspQI - SapI (477) Acc65I - BanI (507) KpnI (511) BstBI (995) PflFI - Tth111I (1027) BamHI (1051) Eco53kI (1162) BanII - SacI (1164) EcoRV (1168) ScaI (1203) BtgI - NcoI - StyI (1315) EaeI (1351) pWS1859 2703 bp

Sequence View

NdeI 5ʹ 3ʹ 60 SRSHMEDGKQAINEGSNDAS a g t c g g t c t c a t a t g g a g g a c g g t a a a c a g g c c a t c a a t g a g g g a t c a a a c g a t g c t t c g t c a g c c a g a g t a t a c c t c c t g c c a t t t g t c c g g t a g t t a c t c c c t a g t t t g c t a c g a a g c

BmgBI 120 PDLDVNGTILMNNEDFSQWS c c g g a t c t g g a c g t g a a t g g c a c a a t a t t g a t g a a t a a t g a a g a c t t t t c c c a g t g g t c g g g c c t a g a c c t g c a c t t a c c g t g t t a t a a c t a c t t a t t a c t t c t g a a a a g g g t c a c c a g c

XcmI 180 VDDVITWCISTLEVEETDPL g t t g a t g a t g t g a t a a c t t g g t g t a t a t c c a c g c t g g a g g t g g a a g a a a c c g a t c c a t t a c a a c t a c t a c a c t a t t g a a c c a c a t a t a g g t g c g a c c t c c a c c t t c t t t g g c t a g g t a a t

BglII 240 CQRLRENDIVGDLLPELCLQ t g t c a g a g a c t g c g a g a a a a t g a t a t t g t a g g a g a t c t t t t g c c g g a a t t g t g c t t g c a a a c a g t c t c t g a c g c t c t t t t a c t a t a a c a t c c t c t a g a a a a c g g c c t t a a c a c g a a c g t t

300 DCQDLCDGDLNKAIKFKILI g a t t g c c a g g a c t t g t g t g a c g g t g a t t t g a a t a a g g c c a t a a a a t t c a a g a t a c t g a t c c t a a c g g t c c t g a a c a c a c t g c c a c t a a a c t t a t t c c g g t a t t t t a a g t t c t a t g a c t a g

360 NKMRDSKLEWKDDKTQEDMI a a t a a g a t g a g a g a c a g c a a g t t g g a g t g g a a g g a c g a c a a g a c t c a a g a g g a c a t g a t a t t a t t c t a c t c t c t g t c g t t c a a c c t c a c c t t c c t g c t g t t c t g a g t t c t c c t g t a c t a t

BsrGI 420 TVLKNLYTTTSAKLQEFQSQ a c g g t a c t g a a a a a c t t g t a c a c t a c t a c a t c t g c g a a a t t g c a a g a a t t t c a a t c g c a g t g c c a t g a c t t t t t g a a c a t g t g a t g a t g t a g a c g c t t t a a c g t t c t t a a a g t t a g c g t c

BspQISapIBbvCI 480 YTRLRMDVLDVMKTSSSSSP t a c a c a a g g c t g a g g a t g g a t g t c t t g g a c g t a a t g a a g a c c a g c t c a a g c t c t t c t c c g a t g t g t t c c g a c t c c t a c c t a c a g a a c c t g c a t t a c t t c t g g t c g a g t t c g a g a a g a g g c

Acc65IBanIKpnI 540 INTHGVSTTVPSSNNTIIPS a t t a a c a c a c a t g g a g t g t c c a c t a c g g t a c c t t c t t c a a a c a a c a c a a t t a t a c c c a g t t a a t t g t g t g t a c c t c a c a g g t g a t g c c a t g g a a g a a g t t t g t t g t g t t a a t a t g g g t c a

600 SDGVSLSQTDYFDTVHNRQS a g t g a c g g t g t g t c t c t t t c a c a a a c a g a c t a t t t c g a c a c a g t t c a t a a c c g a c a a t c a t c a c t g c c a c a c a g a g a a a g t g t t t g t c t g a t a a a g c t g t g t c a a g t a t t g g c t g t t a g t

660 PSRRESPVTVFRQPSLSHSK c c g t c a a g g a g a g a a t c c c c g g t a a c g g t a t t t a g g c a a c c c a g t c t t t c c c a c t c a a a a g g c a g t t c c t c t c t t a g g g g c c a t t g c c a t a a a t c c g t t g g g t c a g a a a g g g t g a g t t t t

720 SLHKDSKNKVPQISTNQSHP t c t t t g c a c a a g g a t a g c a a a a a c a a a g t a c c c c a a a t a t c t a c a a a c c a a t c t c a c c c a a g a a a c g t g t t c c t a t c g t t t t t g t t t c a t g g g g t t t a t a g a t g t t t g g t t a g a g t g g g t

780 SAVSTANTPGPSPNEALKQL t c t g c c g t t t c a a c a g c g a a c a c a c c g g g g c c a t c a c c t a a c g a g g c g t t a a a a c a g t t g a g a c g g c a a a g t t g t c g c t t g t g t g g c c c c g g t a g t g g a t t g c t c c g c a a t t t t g t c a a c

840 RASKEDSCERILKNAMKRHN c g t g c a t c t a a a g a a g a c t c c t g c g a a c g g a t c t t g a a a a a c g c a a t g a a a a g a c a t a a c g c a c g t a g a t t t c t t c t g a g g a c g c t t g c c t a g a a c t t t t t g c g t t a c t t t t c t g t a t t g

900 LADQDWRQYVLVICYGDQER t t a g c a g a t c a g g a t t g g a g a c a a t a t g t c t t g g t c a t t t g c t a t g g g g a t c a a g a g a g g a a t c g t c t a g t c c t a a c c t c t g t t a t a c a g a a c c a g t a a a c g a t a c c c c t a g t t c t c t c c

960 LLELNEKPVIIFKNLKQQGL c t g t t a g a a t t g a a c g a a a a g c c t g t g a t c a t a t t c a a g a a c t t a a a g c a a c a g g g t t t g g a c a a t c t t a a c t t g c t t t t c g g a c a c t a g t a t a a g t t c t t g a a t t t c g t t g t c c c a a a c

BstBI 1020 HPAIMLRRRGDFEEVAMMNG c a c c c c g c c a t t a t g t t a a g a a g a a g a g g t g a t t t c g a a g a a g t a g c a a t g a t g a a c g g a g t g g g g c g g t a a t a c a a t t c t t c t t c t c c a c t a a a g c t t c t t c a t c g t t a c t a c t t g c c t

BamHITth111IPflFI 1080 lambda t0 terminator SDNVTPGGRLGS* a g t g a c a a t g t c a c c c c c g g t g g a a g a t t g g g a t c c t g a g a c c a g a c c a a t a a a a a a c g c t c a c t g t t a c a g t g g g g g c c a c c t t c t a a c c c t a g g a c t c t g g t c t g g t t a t t t t t t g c g

1140 lambda t0 terminator c c g g c g g c a a c c g a g c g t t c t g a a c a a a t c c a g a t g g a g t t c t g a g g t c a t t a c t g g a t c g g c c g c c g t t g g c t c g c a a g a c t t g t t t a g g t c t a c c t c a a g a c t c c a g t a a t g a c c t a g

Eco53kIBanIISacIEcoRV 1200 220 215 *AGGQWEDC CmR *AGGQWEDC t a t c a a c a g g a g t c c a a g c g a g c t c g a t a t c a a a t t a c g c c c c g c c c t g c c a c t c a t c g c a t a g t t g t c c t c a g g t t c g c t c g a g c t a t a g t t t a a t g c g g g g c g g g a c g g t g a g t a g c g

ScaI 1260 210 205 200 195 YQQLENLMRGVHFGDCVAHH CmR YQQLENLMRGVHFGDCVAHH a g t a c t g t t g t a a t t c a t t a a g c a t t c t g c c g a c a t g g a a g c c a t c a c a a a c g g c a t g a t t c a t g a c a a c a t t a a g t a a t t c g t a a g a c g g c t g t a c c t t c g g t a g t g t t t g c c g t a c t a

NcoIStyIBtgI 1320 190 185 180 175 VQIALPMLVKDGQTYYKGMT CmR VQIALPMLVKDGQTYYKGMT g a a c c t g a a t c g c c a g c g g c a t c a g c a c c t t g t c g c c t t g c g t a t a a t a t t t g c c c a t g g c t t g g a c t t a g c g g t c g c c g t a g t c g t g g a a c a g c g g a a c g c a t a t t a t a a a c g g g t a c c

EaeIMscI 1380 170 165 160 155 FVPAFFNDMNAVNLDFSTFS CmR FVPAFFNDMNAVNLDFSTFS t g a a a a c g g g g g c g a a g a a g t t g t c c a t a t t g g c c a c g t t t a a a t c a a a a c t g g t g a a a c a c t t t t g c c c c c g c t t c t t c a a c a g g t a t a a c c g g t g c a a a t t t a g t t t t g a c c a c t t t g

PflMI*PasI 1440 150 145 140 135 VWPNASVFFMNEIFGKPFYA CmR VWPNASVFFMNEIFGKPFYA t c a c c c a g g g a t t g g c t g a a a c g a a a a a c a t a t t c t c a a t a a a c c c t t t a g g g a a a t a g g a g t g g g t c c c t a a c c g a c t t t g c t t t t t g t a t a a g a g t t a t t t g g g a a a t c c c t t t a t c c

1500 130 125 120 115 LNEGYCAVDQSYIHLFQRFD CmR LNEGYCAVDQSYIHLFQRFD c c a g g t t t t c a c c g t a a c a c g c c a c a t c t t g c g a a t a t a t g t g t a g a a a c t g c c g g a a a t g g t c c a a a a g t g g c a t t g t g c g g t g t a g a a c g c t t a t a t a c a c a t c t t t g a c g g c c t t t a

AclI 1560 110 105 100 95 DHYESWLSSFTETQEHFVTY CmR DHYESWLSSFTETQEHFVTY c g t c g t g g t a t t c a c t c c a g a g c g a t g a a a a c g t t t c a g t t t g c t c a t g g a a a a c g g t g t g c a g c a c c a t a a g t g a g g t c t c g c t a c t t t t g c a a a g t c a a a c g a g t a c c t t t t g c c a c a

1620 90 85 80 75 CPHVSDWIVLEGDKMAMRFE CmR CPHVSDWIVLEGDKMAMRFE a a c a a g g g t g a a c a c t a t c c c a t a t c a c c a g c t c a c c g t c t t t c a t t g c c a t a c g a a a t t t t g t t c c c a c t t g t g a t a g g g t a t a g t g g t c g a g t g g c a g a a a g t a a c g g t a t g c t t t a a

BspEI 1680 70 65 60 55 PHANMLRALIHIFAPYFKHK CmR PHANMLRALIHIFAPYFKHK c c g g a t g a g c a t t c a t c a g g c g g g c a a g a a t g t g a a t a a a g g c c g g a t a a a a c t t g t g c t g g c c t a c t c g t a a g t a g t c c g c c c g t t c t t a c a c t t a t t t c c g g c c t a t t t t g a a c a c g a

PvuIITsoI 1740 50 45 40 35 NKKVTKLFATIDLQVTQNYT CmR NKKVTKLFATIDLQVTQNYT t a t t t t t c t t t a c g g t c t t t a a a a a g g c c g t a a t a t c c a g c t g a a c g g t c t g g t t a t a g g a t a a a a a g a a a t g c c a g a a a t t t t t c c g g c a t t a t a g g t c g a c t t g c c a g a c c a a t a t c c

1800 30 25 20 15 CQAVSQFAEFHEKRHWQSID CmR G C A A C T G A C T G A A A T G C C T C CAT-R CQAVSQFAEFHEKRHWQSID t a c a t t g a g c a a c t g a c t g a a a t g c c t c a a a a t g t t c t t t a c g a t g c c a t t g g g a t a t a t a t g t a a c t c g t t g a c t g a c t t t a c g g a g t t t t a c a a g a a a t g c t a c g g t a a c c c t a t a t a

1860 10 5 1 VTTYGTIKKEM CmR VTTYGTIKKEM c a a c g g t g g t a t a t c c a g t g a t t t t t t t c t c c a t t t t a g c t t c c t t a g c t c c t g a a a a t c g t t g c c a c c a t a t a g g t c a c t a a a a a a a g a g g t a a a a t c g a a g g a a t c g a g g a c t t t t a g

MslI 1920 t c g a t a a c t c a a a a a a t a c g c c c g g t a g t g a t c t t a t t t c a t t a t g g t g a a a g t t g g a a c a g c t a t t g a g t t t t t t a t g c g g g c c a t c a c t a g a a t a a a g t a a t a c c a c t t t c a a c c t t g

BspHIBsaAI 1980 c t c t t a c g t g c c c g a t c a a t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g a g a a t g c a c g g g c t a g t t a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t

2040 ori g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t

2100 ori a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t

AcuI 2160 ori g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a

2220 ori g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t

2280 ori t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a

BsiEI 2340 ori a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c

BseYIPspFIApaLI 2400 ori g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c

HaeII 2460 ori c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t

BssSαIBssSIBciVI 2520 ori G G G A A A C G C C T G G T A T pBR322ori-F a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a

DrdI 2580 ori C T T T pBR322ori-F c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c

2640 ori t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g

TaqIIAflIIIPciINspI 2700 MFFPALSPDSVDN t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g

3ʹ 5ʹ 2703 R c g t g c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.