Addgene 014LP-pAAV-ANF-657- Cre-pA Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: 014LP-pAAV-ANF-657- Cre-pA Sequencing Result


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F1ori-R (4727 .. 4746) NaeI (4705) NgoMIV (4703) DraIII (4602) F1ori-F (4515 .. 4536) XmnI (4059) Amp-R (4010 .. 4029) ScaI (3940) BsaI (3521) BmrI (3500) AlwNI (2983) pBR322ori-F (2708 .. 2727) AflIII - PciI (2567) AflII (174) NheI (332) BmtI (336) BsgI (474) PpuMI (489) Bsu36I (515) BbvCI - Bpu10I (621) HindIII (677) BstAPI (713) BsmBI - Esp3I (826) EcoRI (837) XbaI (850) BstXI (869) BsaBI * (871) BamHI (872) Acc65I (881) KpnI (885) BmgBI (1120) BfuAI - BspMI (1140) CsiI - SexAI * (1338) PflFI - Tth111I (1340) StuI (1544) PasI (1552) BtgI - NcoI (1911) HA NotI (1971) EBV-rev (2004 .. 2023) SV40pA-R (2058 .. 2077) HincII - HpaI (2114) SnaBI (2224) BspQI - SapI (2451) 014LP-pAAV-ANF-657- Cre-pA 4901 bp

Sequence View

60 AAV2 ITR c a g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c g t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g

120 AAV2 ITR t t t g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a a a c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g

AflII 180 AAV2 ITR a c t a g g g g t t c c t t g t a g t t a a t g a t t a a c c c g c c a t g c t a c t t a t c t a c a t c c t t a a g g t g a t c c c c a a g g a a c a t c a a t t a c t a a t t g g g c g g t a c g a t g a a t a g a t g t a g g a a t t c c

240 g g c c c t g t a t c a t g t t g g c t t c c t g g c t g a c t t c a t a c t c t a a a a a a a a a a t a a t a g c t c c c g g g a c a t a g t a c a a c c g a a g g a c c g a c t g a a g t a t g a g a t t t t t t t t t t a t t a t c g a g

300 t t t c a c c t g a c t g c t a a c a g g g a c a t c t a g t g t g g g g g t g g g c t g t c t g g g g c c a g a g g t a a a g t g g a c t g a c g a t t g t c c c t g t a g a t c a c a c c c c c a c c c g a c a g a c c c c g g t c t c c a

BmtINheI 360 c c a c c c a c g a g g c c g a t g a a t c a g g t g t g a a g c t a g c t c c a g c a t g t g t a c t c c c t g g c c g g t g g g t g c t c c g g c t a c t t a g t c c a c a c t t c g a t c g a g g t c g t a c a c a t g a g g g a c c g g

420 a g c c t a g c t g g c c t c c c a g c t g c c t g t c a t t g c c t c c t c t c c c g c c c t t a t t t g g a g c c c t c g g a t c g a c c g g a g g g t c g a c g g a c a g t a a c g g a g g a g a g g g c g g g a a t a a a c c t c g g g

BsgI 480 c t g a c a g c t g a g c a g c a a g c a g g g g g a g c t g g g t g c a g g c c a g c c g t c a c c c t c t g c t t c g a c t g t c g a c t c g t c g t t c g t c c c c c t c g a c c c a c g t c c g g t c g g c a g t g g g a g a c g a a g

Bsu36IPpuMI 540 c c t g c a t g g g t c c t g t t g c c a g g g a g a a a g a a t c c t g a g g c g a g c g c c c a g g a a g a t a a c g g a c g t a c c c a g g a c a a c g g t c c c t c t t t c t t a g g a c t c c g c t c g c g g g t c c t t c t a t t g

600 c a a g g a c t c t t t t c t g c t c c t c t c a c a c c t t t g a a g t g g g g g c c t c t t g a g g c a a a t c a g g t t c c t g a g a a a a g a c g a g g a g a g t g t g g a a a c t t c a c c c c c g g a g a a c t c c g t t t a g t c

Bpu10IBbvCI 660 c a a g a a t g t g a c t c t t g c a g c t g a g g g t c t g g g g g a g g g g g g t g a g t g g a g c t g c t c a a g g t t c t t a c a c t g a g a a c g t c g a c t c c c a g a c c c c c t c c c c c c a c t c a c c t c g a c g a g t t c

BstAPIHindIII 720 g c a a a g g g g c c g t g a c a a g c t t t g c c g a a c t g a t a a c t t t a a a a g g g c a t c t t c t g c t g g c g t t t c c c c g g c a c t g t t c g a a a c g g c t t g a c t a t t g a a a t t t t c c c g t a g a a g a c g a c c

780 c t c c t c a c t c c a t c g c t t t a t c g c t g c a a g t g a c a g a a t g g g g a g g g t t c t a g c c c c c c t g a g g a g t g a g g t a g c g a a a t a g c g a c g t t c a c t g t c t t a c c c c t c c c a a g a t c g g g g g g a

EcoRIBsmBIEsp3I 840 MLAARRRI g c c t t c t c a a a g a g c t g g g g g g c t a t a a a a a c g g g a g a t g c t g g c a g c t a g g a g a c g a a t c g g a a g a g t t t c t c g a c c c c c c g a t a t t t t t g c c c t c t a c g a c c g t c g a t c c t c t g c t t a

BstXIBsaBI*BamHIAcc65IKpnIXbaI 900 PKK SV40 NLS PDPLENIQGSGSKGTTMPKK t c c a g a c c c t c t a g a a a a t a t c c a a g g a t c t g g a t c c a a a g g t a c c a c c a t g c c c a a g a a a g g t c t g g g a g a t c t t t t a t a g g t t c c t a g a c c t a g g t t t c c a t g g t g g t a c g g g t t c t t

960 130 KRKV (in frame with SV40 NLS) SNLLTVHQNLPALPVD SV40 NLS KRKVSNLLTVHQNLPALPVD g a a g a g g a a g g t g t c c a a t c t c c t g a c t g t t c a c c a g a a c c t c c c t g c g c t g c c a g t a g a c t t c t c c t t c c a c a g g t t a g a g g a c t g a c a a g t g g t c t t g g a g g g a c g c g a c g g t c a t c t

1020 (in frame with SV40 NLS) ATSDEVRKNLMDMFRDRQAF ATSDEVRKNLMDMFRDRQAF t g c c a c t a g c g a t g a g g t c a g g a a a a a t c t c a t g g a t a t g t t t a g g g a t a g a c a g g c g t t a c g g t g a t c g c t a c t c c a g t c c t t t t t a g a g t a c c t a t a c a a a t c c c t a t c t g t c c g c a a

1080 (in frame with SV40 NLS) SEHTWKMLLSVCRSWAAWCK SEHTWKMLLSVCRSWAAWCK t t c t g a a c a c a c c t g g a a a a t g c t g c t t a g c g t g t g c c g a t c c t g g g c a g c c t g g t g t a a a a g a c t t g t g t g g a c c t t t t a c g a c g a a t c g c a c a c g g c t a g g a c c c g t c g g a c c a c a t t

BmgBI 1140 (in frame with SV40 NLS) LNNRKWFPAEPEDVRDYLLY LNNRKWFPAEPEDVRDYLLY g c t g a a c a a t c g c a a a t g g t t c c c c g c c g a g c c g g a g g a c g t g c g c g a t t a c c t g c t g t a c g a c t t g t t a g c g t t t a c c a a g g g g c g g c t c g g c c t c c t g c a c g c g c t a a t g g a c g a c a t

BspMIBfuAI 1200 (in frame with SV40 NLS) LQARGLAVKTIQQHLGQLNM LQARGLAVKTIQQHLGQLNM t c t c c a g g c a a g a g g g c t g g c t g t c a a g a c t a t c c a g c a g c a c t t g g g c c a a c t g a a t a t a g a g g t c c g t t c t c c c g a c c g a c a g t t c t g a t a g g t c g t c g t g a a c c c g g t t g a c t t a t a

1260 (in frame with SV40 NLS) LHRRSGLPRPSDSNAVSLVM LHRRSGLPRPSDSNAVSLVM g c t g c a t c g a c g c a g c g g g c t c c c c c g g c c t a g c g a t t c a a a c g c a g t c t c c c t t g t t a t c g a c g t a g c t g c g t c g c c c g a g g g g g c c g g a t c g c t a a g t t t g c g t c a g a g g g a a c a a t a

1320 (in frame with SV40 NLS) RRIRKENVDAGERAKQALAF RRIRKENVDAGERAKQALAF g a g g a g a a t t a g a a a g g a a a a c g t a g a t g c g g g t g a g a g g g c t a a g c a g g c t c t c g c t t t c t c c t c t t a a t c t t t c c t t t t g c a t c t a c g c c c a c t c t c c c g a t t c g t c c g a g a g c g a a a

SexAI*CsiITth111IPflFI 1380 (in frame with SV40 NLS) ERTDFDQVRSLMENSDRCQD ERTDFDQVRSLMENSDRCQD t g a g c g g a c t g a t t t c g a c c a g g t c a g a t c c c t g a t g g a g a a c a g c g a t c g g t g c c a g g a a c t c g c c t g a c t a a a g c t g g t c c a g t c t a g g g a c t a c c t c t t g t c g c t a g c c a c g g t c c t

1440 (in frame with SV40 NLS) IRNLAFLGIAYNTLLRIAEI IRNLAFLGIAYNTLLRIAEI c a t c a g g a a c c t c g c a t t t c t g g g a a t t g c a t a t a a c a c a c t t c t g c g c a t a g c t g a g a t g t a g t c c t t g g a g c g t a a a g a c c c t t a a c g t a t a t t g t g t g a a g a c g c g t a t c g a c t c t a

1500 (in frame with SV40 NLS) ARIRVKDISRTDGGRMLIHI ARIRVKDISRTDGGRMLIHI c g c c c g g a t c a g a g t g a a a g a c a t c a g t c g a a c g g a c g g c g g c c g g a t g c t t a t t c a t a t g c g g g c c t a g t c t c a c t t t c t g t a g t c a g c t t g c c t g c c g c c g g c c t a c g a a t a a g t a t a

PasIStuI 1560 (in frame with SV40 NLS) GRTKTLVSTAGVEKALSLGV GRTKTLVSTAGVEKALSLGV t g g a c g c a c a a a g a c a t t g g t c a g c a c c g c t g g c g t t g a a a a g g c c t t g t c c c t g g g c g t a c c t g c g t g t t t c t g t a a c c a g t c g t g g c g a c c g c a a c t t t t c c g g a a c a g g g a c c c g c a

1620 (in frame with SV40 NLS) TKLVERWISVSGVADDPNNY TKLVERWISVSGVADDPNNY a a c g a a g c t g g t g g a a a g a t g g a t c t c a g t g t c c g g c g t g g c t g a c g a c c c t a a t a a t t a t t g c t t c g a c c a c c t t t c t a c c t a g a g t c a c a g g c c g c a c c g a c t g c t g g g a t t a t t a a t

1680 (in frame with SV40 NLS) LFCRVRKNGVAAPSATSQLS LFCRVRKNGVAAPSATSQLS c t t g t t c t g t c g a g t g a g a a a a a a c g g a g t c g c c g c g c c c t c t g c c a c c a g c c a a t t g a g g a a c a a g a c a g c t c a c t c t t t t t t g c c t c a g c g g c g c g g g a g a c g g t g g t c g g t t a a c t c

1740 (in frame with SV40 NLS) TRALEGIFEATHRLIYGAKD TRALEGIFEATHRLIYGAKD t a c a c g g g c c c t t g a a g g g a t c t t t g a g g c a a c c c a c c g a c t c a t a t a c g g a g c c a a g g a a t g t g c c c g g g a a c t t c c c t a g a a a c t c c g t t g g g t g g c t g a g t a t a t g c c t c g g t t c c t

1800 (in frame with SV40 NLS) DSGQRYLAWSGHSARVGAAR DSGQRYLAWSGHSARVGAAR t g a c a g t g g c c a g a g g t a t c t c g c c t g g t c a g g t c a t t c t g c t a g g g t g g g g g c c g c a c g a c t g t c a c c g g t c t c c a t a g a g c g g a c c a g t c c a g t a a g a c g a t c c c a c c c c c g g c g t g c

1860 (in frame with SV40 NLS) DMARAGVSIPEIMQAGGWTN DMARAGVSIPEIMQAGGWTN a g a c a t g g c g c g g g c a g g a g t c t c c a t a c c a g a g a t t a t g c a a g c t g g a g g t t g g a c a a a t c t g t a c c g c g c c c g t c c t c a g a g g t a t g g t c t c t a a t a c g t t c g a c c t c c a a c c t g t t t

NcoIBtgI 1920 (in frame with SV40 NLS) VNIVMNYIRNLDSETGAMVR VNIVMNYIRNLDSETGAMVR t g t g a a c a t c g t t a t g a a c t a t a t c c g c a a t c t t g a c t c t g a a a c c g g g g c c a t g g t g a g a c a c t t g t a g c a a t a c t t g a t a t a g g c g t t a g a a c t g a g a c t t t g g c c c c g g t a c c a c t c

NotI 1980 (in frame with SV40 NLS) LLEDGD 1 5 YPYDVPDYA * HA LLEDGDYPYDVPDYA* a c t g c t c g a a g a t g g t g a c t a c c c a t a c g a t g t t c c a g a t t a c g c t t g a g c g g c c g c t t c t g a c g a g c t t c t a c c a c t g a t g g g t a t g c t a c a a g g t c t a a t g c g a a c t c g c c g g c g a a g

2040 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev g a g c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a c t c g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t

2100 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a

HincIIHpaI 2160 SV40 poly(A) signal g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c t

2220 t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a g a g c a t g g a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t c t c g t a c c

SnaBI 2280 AAV2 ITR c t a c g t a g a t a a g t a g c a t g g c g g g t t a a t c a t t a a c t a c a a g g a a c c c c t a g t g a t g g a g a t g c a t c t a t t c a t c g t a c c g c c c a a t t a g t a a t t g a t g t t c c t t g g g g a t c a c t a c c t

2340 AAV2 ITR g t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g

2400 AAV2 ITR c c g a c g c c c g g g c t t t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c t g c a t t a a g g c t g c g g g c c c g a a a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g a c g t a a t t

BspQISapI 2460 t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c

2520 c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c

AflIIIPciI 2580 g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t

2640 ori g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g

2700 ori c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t

2760 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c

2820 ori a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a

2880 ori c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a

2940 ori g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c

AlwNI 3000 ori t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g

3060 ori a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g

3120 ori a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t

3180 ori g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g

3240 ori a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c

3300 g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t

3360 a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a

3420 285 280 *WHKILSAGIE AmpR *WHKILSAGIE a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t

3480 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c

BsaIBmrI 3540 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t

3600 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a

3660 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a

3720 195 190 185 180 LEGTLLKRLTTAMAVPMTTD AmpR LEGTLLKRLTTAMAVPMTTD a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t

3780 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t

3840 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t

3900 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a

ScaI 3960 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t

4020 95 90 85 80 SYHIRRGLQEQGADIRSLVA AmpR A T A A T A C C G C G Amp-R SYHIRRGLQEQGADIRSLVA g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c

XmnI 4080 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g

4140 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t

4200 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a

4260 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a

4320 AmpR promoter c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a

4380 AmpR promoter f1 ori a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t

4440 f1 ori t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a

4500 f1 ori t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c

4560 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c

DraIII 4620 f1 ori t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a

4680 f1 ori c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g

NgoMIVNaeI 4740 f1 ori A G G G A A G A A A G C G A F1ori-R g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t

4800 f1 ori A A G G A G F1ori-R a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g

4860 f1 ori c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c a g g c t g c g c a a c t g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g t c c g a c g c g t t g a

4901 g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.