Addgene EF1a_FOXP1_P2A_Hygro_Barcode Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: EF1a_FOXP1_P2A_Hygro_Barcode Sequencing Result


Map View

SgrDI (0) SspI (9731) Amp-R (9477 .. 9496) FspI (9149) AhdI (8927) pBR322ori-F (8175 .. 8194) L4440 (7924 .. 7941) CAP binding site lac promoter M13/pUC Reverse (7706 .. 7728) lac operator M13 Reverse (7693 .. 7709) M13 rev BstZ17I (7655) EBV-rev (7614 .. 7633) SV40pA-R (7560 .. 7579) BleoR BmgBI (7153) SgrAI (7133) MauBI (7055) MscI (7022) EM7 promoter AvrII (6890) SV40pro-F (6818 .. 6837) pBABE 3' (6571 .. 6591) F1ori-F (6431 .. 6452) F1ori-R (6221 .. 6240) BGH-rev (5858 .. 5875) PmeI (5842) 5' LTR (truncated) BmtI (5634) NheI (5630) loxP XbaI * (5442) pRS-marker (46 .. 65) MluI (230) SpeI (251) CMV enhancer SnaBI (592) CMV-F (771 .. 791) NotI (1524) AleI (1953) KflI (2310) PacI (2597) BspDI - ClaI (2601) AgeI (2770) PasI (3440) PaeR7I - XhoI (3657) EF1a-F (3818 .. 3838) T7 (3887 .. 3906) PflMI (4189) SbfI (4237) PshAI (4404) AsiSI (4741) RsrII (4785) EF1a_FOXP1_P2A_Hygro_Barcode 9848 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T pRS-marker t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t

120 A C G G C pRS-marker t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c

180 c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a

MluI 240 CMV enhancer g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t

SpeI 300 CMV enhancer t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t

360 CMV enhancer t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t

420 CMV enhancer c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a

480 CMV enhancer c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a

540 CMV enhancer g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t

SnaBI 600 CMV enhancer t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a

660 CMV enhancer CMV promoter c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a

720 CMV promoter t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t

780 CMV promoter C G C A A A T G G G CMV-F c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c

840 CMV promoter 5' LTR (truncated) C G G T A G G C G T G CMV-F c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g

900 5' LTR (truncated) t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t

960 5' LTR (truncated) a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a

1020 5' LTR (truncated) c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c

1080 HIV-1 Ψ c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c

1140 HIV-1 Ψ g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a

1200 HIV-1 Ψ t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c

1260 g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t

1320 a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a

1380 g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g

1440 a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t

1500 a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t

NotI 1560 M a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t

1620 RDNWRSELYKYKVVKIEPLG g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c

1680 RRE VAPTKAKRRVVQREKRAVGI t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c

1740 RRE GALFLGFLGAAGSTMGAASM g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t

1800 RRE TLTVQARQLLSGIVQQQNNL c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g

1860 RRE LRAIEAQQHLLQLTVWGIKQ t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g

1920 RRE LQARILAVERYLKDQQLLGI t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a

AleI 1980 WGCSGKLICTTAVPWNASWS g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t

2040 NKSLEQIWNHTTWMEWDREI a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t

2100 1 K gp41 peptide NNYTSLIHSLIEESQNQQEK a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t

2160 5 10 15 NEQELLELDKWASL (in frame with gp41 peptide) WNWFNI gp41 peptide NEQELLELDKWASLWNWFNI a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t

2220 (in frame with gp41 peptide) TNWLWYIKLFIMIVGGLVGL TNWLWYIKLFIMIVGGLVGL c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t

2280 (in frame with gp41 peptide) RIVFAVLSIVNRVRQGYSPL RIVFAVLSIVNRVRQGYSPL g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a

KflI 2340 (in frame with gp41 peptide) SFQTHLPTPRGPDRPEGIEE SFQTHLPTPRGPDRPEGIEE c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c

2400 (in frame with gp41 peptide) EGGERDRDRSIRLVNGSALR EGGERDRDRSIRLVNGSALR a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c

2460 (in frame with gp41 peptide) APILQTNGSIHPQF* cPPT/CTS APILQTNGSIHPQF* c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a

2520 cPPT/CTS g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t

2580 cPPT/CTS g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t

BspDIClaIPacI 2640 g a t c c a g t t t g g t t a a t t a a t c g a t a a g c t t t g c a a a g a t g g a t a a a g t t t t a a a c a g a g c t a g g t c a a a c c a a t t a a t t a g c t a t t c g a a a c g t t t c t a c c t a t t t c a a a a t t t g t c t c

2700 EF-1 α promoter a g g a a t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t t c c t t a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a

2760 EF-1 α promoter g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g

AgeI 2820 EF-1 α promoter g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c

2880 EF-1 α promoter t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g

2940 EF-1 α promoter EF-1 α intron A g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a

3000 EF-1 α promoter EF-1 α intron A *KWR t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g

3060 EF-1 α promoter EF-1 α intron A AATRSEQDRAEPQFHTPSNS t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c

3120 EF-1 α promoter EF-1 α intron A AKRKLLGKAEHKLQPRAQAS a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g

3180 EF-1 α promoter EF-1 α intron A PGGRAFRTAGERRDRQKRYT t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c

3240 EF-1 α promoter EF-1 α intron A ELWKFNKIVQQSAKKRALYD t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a

3300 EF-1 α promoter EF-1 α intron A QLHPGLDACQYKPKQPRPRR c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g

3360 EF-1 α promoter EF-1 α intron A RPGHTGACMNPSAPGALAAV g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t

3420 EF-1 α promoter EF-1 α intron A SFRVPTTELQGAQEPAQGRA c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c

PasI 3480 EF-1 α promoter EF-1 α intron A ATYRGARPPLAPGTPVLQTL c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t

3540 EF-1 α promoter EF-1 α intron A PFIAAERGQQLSSLISSAAS g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g

3600 EF-1 α promoter EF-1 α intron A PLAPPHTVWVFSFPRETRLR t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g

PaeR7IXhoI 3660 EF-1 α promoter EF-1 α intron A RKM g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c

3720 EF-1 α promoter EF-1 α intron A a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g

3780 EF-1 α promoter EF-1 α intron A c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c

3840 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t

3900 EF-1 α promoter T7 promoter EF-1 α intron A T A A T A C G A C T C A C T T7 a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t a g c t t g g t a c t a a t a c g a c t c a c t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a t c g a a c c a t g a t t a t g c t g a g t g a

3960 T7 promoter A T A G G G T7 a t a g g g a g a c c c a a g c t g g c t a g g t a a g c t t g a t a t c g a a t t c c t g c a g c c c g g g g g a t c t a t c c c t c t g g g t t c g a c c g a t c c a t t c g a a c t a t a g c t t a a g g a c g t c g g g c c c c c t a g

4020 MMQESGTETKSNGSAIQ c c g c c a c c a t g a t g c a a g a a t c t g g g a c t g a g a c a a a a a g t a a c g g t t c a g c c a t c c a g a g g c g g t g g t a c t a c g t t c t t a g a c c c t g a c t c t g t t t t t c a t t g c c a a g t c g g t a g g t c t

4080 NGSGGSNHLLECGGLREGRS a t g g g t c g g g c g g c a g c a a c c a c t t a c t a g a g t g c g g c g g t c t t c g g g a g g g g c g g t c c a t a c c c a g c c c g c c g t c g t t g g t g a a t g a t c t c a c g c c g c c a g a a g c c c t c c c c g c c a g g t

4140 NGETPAVDIGAADLAHAQQQ a c g g a g a g a c g c c g g c c g t g g a c a t c g g g g c a g c t g a c c t c g c c c a c g c c c a g c a g c a g c t g c c t c t c t g c g g c c g g c a c c t g t a g c c c c g t c g a c t g g a g c g g g t g c g g g t c g t c g t c g

PflMI 4200 QQQWHLINHQPSRSPSSWLK a g c a a c a g t g g c a t c t c a t a a a c c a t c a g c c c t c t a g g a g t c c c a g c a g t t g g c t t a a g a t c g t t g t c a c c g t a g a g t a t t t g g t a g t c g g g a g a t c c t c a g g g t c g t c a a c c g a a t t c t

SbfI 4260 RLISSPWELEVLQVPLWGAV *ARPLQFDQLDGQPSCN g a c t a a t t t c a a g c c c t t g g g a g t t g g a a g t c c t g c a g g t c c c c t t g t g g g g a g c a g t t g c t g a t t a a a g t t c g g g a a c c c t c a a c c t t c a g g a c g t c c a g g g g a a c a c c c c t c g t c a a c

4320 P2A AETKMSGPVCQPNPSPFGSG SLRLHTSRHTLRVRGWKSGA c t g a g a c g a a g a t g a g t g g a c c t g t g t g t c a g c c t a a c c c t t c c c c a t t t g g a t c c g g c g g a c t c t g c t t c t a c t c a c c t g g a c a c a c a g t c g g a t t g g g a a g g g g t a a a c c t a g g c c g c

4380 1 5 10 15 ATNFSLLKQAGDVEENPGP 1 M P2A HygR 1 M HygR ATNFSLLKQAGDVEENPGPM CCVERQQFLGSIDFLIRSRH c a a c a a a c t t c t c t c t g c t g a a a c a a g c c g g a g a t g t c g a a g a g a a t c c t g g a c c g a t g a g t t g t t t g a a g a g a g a c g a c t t t g t t c g g c c t c t a c a g c t t c t c t t a g g a c c t g g c t a c t

PshAI 4440 5 10 15 20 KKPELTATSVEKFLIEKFDS HygR 5 10 15 20 KKPELTATSVEKFLIEKFDS HygR KKPELTATSVEKFLIEKFDS FLRFEGRRRDLLKQDFLEVA a a a a g c c t g a a c t c a c c g c g a c g t c t g t c g a g a a g t t t c t g a t c g a a a a g t t c g a c a g c g t t t t c g g a c t t g a g t g g c g c t g c a g a c a g c t c t t c a a a g a c t a g c t t t t c a a g c t g t c g c

4500 25 30 35 40 VSDLMQLSEGEESRAFSFDV HygR 25 30 35 40 VSDLMQLSEGEESRAFSFDV HygR VSDLMQLSEGEESRAFSFDV DGVQHLERLAFFRTSEAEIY t c t c c g a c c t g a t g c a g c t c t c g g a g g g c g a a g a a t c t c g t g c t t t c a g c t t c g a t g t a g a g a g g c t g g a c t a c g t c g a g a g c c t c c c g c t t c t t a g a g c a c g a a a g t c g a a g c t a c a t c

4560 45 50 55 60 GGRGYVLRVNSCADGFYKDR HygR 45 50 55 60 GGRGYVLRVNSCADGFYKDR HygR GGRGYVLRVNSCADGFYKDR SPTSIDQPYIAAGITEVFIT g a g g g c g t g g a t a t g t c c t g c g g g t a a a t a g c t g c g c c g a t g g t t t c t a c a a a g a t c g t t c t c c c g c a c c t a t a c a g g a c g c c c a t t t a t c g a c g c g g c t a c c a a a g a t g t t t c t a g c a a

4620 65 70 75 80 YVYRHFASAALPIPEVLDIG HygR 65 70 75 80 YVYRHFASAALPIPEVLDIG HygR YVYRHFASAALPIPEVLDIG INIPVKCRGRERNRFHKVNP a t g t t t a t c g g c a c t t t g c a t c g g c c g c g c t c c c g a t t c c g g a a g t g c t t g a c a t t g g g g t a c a a a t a g c c g t g a a a c g t a g c c g g c g c g a g g g c t a a g g c c t t c a c g a a c t g t a a c c c c

4680 85 90 95 100 EFSESLTYCISRRAQGVTLQ HygR 85 90 95 100 EFSESLTYCISRRAQGVTLQ HygR EFSESLTYCISRRAQGVTLQ FEALAQGIADGATCLTDRQL a a t t c a g c g a g a g c c t g a c c t a t t g c a t c t c c c g c c g t g c a c a g g g t g t c a c g t t g c a a g t t a a g t c g c t c t c g g a c t g g a t a a c g t a g a g g g c g g c a c g t g t c c c a c a g t g c a a c g t t c

4740 105 110 115 120 DLPETELPAVLQPVAEAMDA HygR 105 110 115 120 DLPETELPAVLQPVAEAMDA HygR DLPETELPAVLQPVAEAMDA VQRFGFQGSNQLRDRLGHIR a c c t g c c t g a a a c c g a a c t g c c c g c t g t t c t g c a g c c g g t c g c g g a g g c c a t g g a t g c g a t g g a c g g a c t t t g g c t t g a c g g g c g a c a a g a c g t c g g c c a g c g c c t c c g g t a c c t a c g c t

RsrIIAsiSI 4800 125 130 135 140 IAAADLSQTSGFGPFGPQGI HygR 125 130 135 140 IAAADLSQTSGFGPFGPQGI HygR IAAADLSQTSGFGPFGPQGI DSRGIKALRAPEAWESRLSD t c g c t g c g g c c g a t c t t a g c c a g a c g a g c g g g t t c g g c c c a t t c g g a c c g c a a g g a a t c g a g c g a c g c c g g c t a g a a t c g g t c t g c t c g c c c a a g c c g g g t a a g c c t g g c g t t c c t t a g c

4860 145 150 155 160 GQYTTWRDFICAIADPHVYH HygR 145 150 155 160 GQYTTWRDFICAIADPHVYH HygR GQYTTWRDFICAIADPHVYH TLVSCPTIEYARNSIGMHIV g t c a a t a c a c t a c a t g g c g t g a t t t c a t a t g c g c g a t t g c t g a t c c c c a t g t g t a t c a c t c a g t t a t g t g a t g t a c c g c a c t a a a g t a t a c g c g c t a a c g a c t a g g g g t a c a c a t a g t g a

4920 165 170 175 180 WQTVMDDTVSASVAQALDEL HygR 165 170 175 180 WQTVMDDTVSASVAQALDEL HygR WQTVMDDTVSASVAQALDEL PLSHHVVGDTRGDRLSEILQ g g c a a a c t g t g a t g g a c g a c a c c g t c a g t g c g t c c g t c g c g c a g g c t c t c g a t g a g c t g a c c g t t t g a c a c t a c c t g c t g t g g c a g t c a c g c a g g c a g c g c g t c c g a g a g c t a c t c g a c t

4980 185 190 195 200 MLWAEDCPEVRHLVHADFGS HygR 185 190 195 200 MLWAEDCPEVRHLVHADFGS HygR MLWAEDCPEVRHLVHADFGS HKPGLVAGFDPVEHVRIEAG t g c t t t g g g c c g a g g a c t g c c c c g a a g t c c g g c a c c t c g t g c a c g c g g a t t t c g g c t c c a a c g a a a c c c g g c t c c t g a c g g g g c t t c a g g c c g t g g a g c a c g t g c g c c t a a a g c c g a g g t

5040 205 210 215 220 NNVLTDNGRITAVIDWSEAM HygR 205 210 215 220 NNVLTDNGRITAVIDWSEAM HygR NNVLTDNGRITAVIDWSEAM VIDQRVIAAYCRDNVPALRH a c a a t g t c c t g a c g g a c a a t g g c c g c a t a a c a g c g g t c a t t g a c t g g a g c g a g g c g a t g t t g t t a c a g g a c t g c c t g t t a c c g g c g t a t t g t c g c c a g t a a c t g a c c t c g c t c c g c t a c a

5100 225 230 235 240 FGDSQYEVANIFFWRPWLAC HygR 225 230 235 240 FGDSQYEVANIFFWRPWLAC HygR FGDSQYEVANIFFWRPWLAC EPIGLVLDGVDEEPPRPQST t c g g g g a t t c c c a a t a c g a g g t c g c c a a c a t c t t c t t c t g g a g g c c g t g g t t g g c t t g t a a g c c c c t a a g g g t t a t g c t c c a g c g g t t g t a g a a g a a g a c c t c c g g c a c c a a c c g a a c a t

5160 245 250 255 260 MEQQTRYFERRHPELAGSPR HygR 245 250 255 260 MEQQTRYFERRHPELAGSPR HygR MEQQTRYFERRHPELAGSPR HLLLRAVELPPM t g g a g c a g c a g a c g c g c t a c t t c g a g c g g a g g c a t c c g g a g c t t g c a g g a t c g c c g c g g c a c c t c g t c g t c t g c g c g a t g a a g c t c g c c t c c g t a g g c c t c g a a c g t c c t a g c g g c g c c g

5220 265 270 275 280 LRAYMLRIGLDQLYQSLVDG HygR 265 270 275 280 LRAYMLRIGLDQLYQSLVDG HygR LRAYMLRIGLDQLYQSLVDG t c c g g g c g t a t a t g c t c c g c a t t g g t c t t g a c c a a c t c t a t c a g a g c t t g g t t g a c g g c a a g g c c c g c a t a t a c g a g g c g t a a c c a g a a c t g g t t g a g a t a g t c t c g a a c c a a c t g c c g t

5280 285 290 295 300 NFDDAAWAQGRCDAIVRSGA HygR 285 290 295 300 NFDDAAWAQGRCDAIVRSGA HygR NFDDAAWAQGRCDAIVRSGA a t t t c g a t g a t g c a g c t t g g g c g c a g g g t c g a t g c g a c g c a a t c g t c c g a t c c g g a g c c g t a a a g c t a c t a c g t c g a a c c c g c g t c c c a g c t a c g c t g c g t t a g c a g g c t a g g c c t c g g c

5340 305 310 315 320 GTVGRTQIARRSAAVWTDGC HygR 305 310 315 320 GTVGRTQIARRSAAVWTDGC HygR GTVGRTQIARRSAAVWTDGC g g a c t g t c g g g c g t a c a c a a a t c g c c c g c a g a a g c g c g g c c g t c t g g a c c g a t g g c t g t g c c t g a c a g c c c g c a t g t g t t t a g c g g g c g t c t t c g c g c c g g c a g a c c t g g c t a c c g a c a c

5400 325 330 335 340 VEVLADSGNRRPSTRPRAKE HygR 325 330 335 340 VEVLADSGNRRPSTRPRAKE HygR VEVLADSGNRRPSTRPRAKE t a g a a g t a c t c g c c g a t a g t g g a a a c c g a c g c c c c a g c a c t c g t c c g a g g g c a a a g g a a t a t c t t c a t g a g c g g c t a t c a c c t t t g g c t g c g g g g t c g t g a g c a g g c t c c c g t t t c c t t a

XbaI* 5460 * HygR * HygR * a a g t t a g a a c t a t t t c c t g g c t g t t a c g c g g g g a t g a t t a c t c t a g a t c c c t a c t g a c g g t t c a a t c t t g a t a a a g g a c c g a c a a t g c g c c c c t a c t a a t g a g a t c t a g g g a t g a c t g c c

5520 g c t t g t t a a a c a g t g a a c c g c c c g c c c c a c g a c c c g c a g c g c c c g a c c g a a a g g a g c g c a c g a a c a a t t t g t c a c t t g g c g g g c g g g g t g c t g g g c g t c g c g g g c t g g c t t t c c t c g c g t

5580 c g a c c c c a t g c a t c g a a c a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g g c t g g g g t a c g t a g c t t g t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c

BmtINheI 5640 loxP a a g a c a a g a t a t c a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g t t a t c g g c t a g c t g g t c t t c t g t t c t a t a g t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c a a t a g c c g a t c g a c c a g

5700 5' LTR (truncated) c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g

5760 5' LTR (truncated) t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c

5820 5' LTR (truncated) c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t

PmeI 5880 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g

5940 bGH poly(A) signal c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g

6000 bGH poly(A) signal a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a

6060 bGH poly(A) signal a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c

6120 bGH poly(A) signal c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a

6180 f1 ori a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c

6240 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a

6300 f1 ori t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t

6360 f1 ori g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a

6420 f1 ori t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c

6480 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a

6540 f1 ori t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a

6600 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a

6660 SV40 promoter c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t

6720 SV40 promoter g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a

6780 SV40 promoter SV40 ori a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a

6840 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c

AvrII 6900 SV40 promoter SV40 ori c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a

6960 SV40 promoter EM7 promoter g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t

7020 1 M EM7 promoter BleoR t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a

MauBIMscI 7080 5 10 15 20 AKLTSAVPVLTARDVAGAVE BleoR g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t

SgrAI 7140 25 30 35 40 FWTDRLGFSRDFVEDDFAGV BleoR g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a

BmgBI 7200 45 50 55 60 VRDDVTLFISAVQDQVVPDN BleoR g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t

7260 65 70 75 80 TLAWVWVRGLDELYAEWSEV BleoR c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a

7320 85 90 95 100 VSTNFRDASGPAMTEIGEQP BleoR c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g

7380 105 110 115 120 WGREFALRDPAGNCVHFVAE BleoR g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t

7440 125 EQD* BleoR g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a

7500 g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a

7560 SV40 poly(A) signal C SV40pA-R g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c

7620 SV40 poly(A) signal G T G G T T T EBV-rev G T T A T C G T A G T G T T T A A A G SV40pA-R c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a

BstZ17I 7680 SV40 poly(A) signal G T C C A A A C T C A T C EBV-rev g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a

7740 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t

7800 -10 -35 lac promoter CAP binding site c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a

7860 CAP binding site c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a

7920 g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g

7980 G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t

8040 c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c

8100 ori a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t

8160 ori t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t

8220 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g

8280 ori t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g

8340 ori g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a

8400 ori g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c

8460 ori t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c

8520 ori g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t

8580 ori c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c

8640 ori a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a

8700 ori t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a

8760 t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c

8820 a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a

8880 285 280 *WHKILSAG AmpR *WHKILSAG c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g

AhdI 8940 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a

9000 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g

9060 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c

9120 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c

FspI 9180 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a

9240 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c

9300 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a

9360 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g

9420 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g

9480 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A Amp-R NQSYHIRRGLQEQGADIRSL a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t

9540 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR T A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c

9600 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g

9660 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c

9720 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a

SspI 9780 AmpR promoter c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a

9840 AmpR promoter t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g

3ʹ 5ʹ 9848 a c c t g a c g t g g a c t g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.