Addgene FUW-hTie1-FLAG Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: FUW-hTie1-FLAG Sequencing Result


Map View

gp41 peptide AleI (12,008) NotI (11,579) CMV-F (10,826 .. 10,846) SnaBI (10,647) NdeI (10,541) MluI (10,285) pRS-marker (10,101 .. 10,120) SgrDI (10,055) AmpR promoter SspI (9938) Amp-R (9684 .. 9703) ScaI (9614) PvuI (9504) AhdI (9134) pBR322ori-F (8382 .. 8401) L4440 (8131 .. 8148) CAP binding site lac promoter M13/pUC Reverse (7913 .. 7935) lac operator M13 Reverse (7900 .. 7916) M13 rev BstZ17I (7862) EBV-rev (7821 .. 7840) SV40pA-R (7767 .. 7786) FseI (7502) SgrAI (7340) MauBI (7262) EM7 promoter SV40pro-F (7025 .. 7044) pBABE 3' (6778 .. 6798) F1ori-F (6638 .. 6659) F1ori-R (6428 .. 6447) PacI (0) hUBCpro-F (1072 .. 1091) XbaI (1258) FspAI (1480) PflFI - Tth111I (1660) AgeI (2252) BlpI (2830) AscI (4715) EcoRI (4722) WPRE-R (4799 .. 4819) KS primer pBluescriptKS (5338 .. 5354) Acc65I (5468) KpnI (5472) BspEI (5846) PmeI (6049) BGH-rev (6065 .. 6082) FUW-hTie1-FLAG 12,652 bp

Sequence View

PacI 5ʹ 3ʹ 60 UbC promoter t a a c c c g t g t c g g c t c c a g a t c t g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c a t t g g g c a c a g c c g a g g t c t a g a c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g

120 UbC promoter c c c c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c g g g g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g

180 UbC promoter t t c c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a a a g g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t

240 UbC promoter g t a t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t c a t a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a

300 UbC promoter t c c a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c a g g t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g

360 UbC promoter t c c g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t a g g c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a

420 UbC promoter t c c g t c g c a g c c g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t a g g c a g c g t c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a

480 UbC promoter g g t g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g c c a c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c

540 UbC promoter a c g g a a g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g c c t t c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g

600 UbC promoter t g a a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a a c t t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t

660 UbC promoter g a c g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a c t g c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t

720 UbC promoter a g a a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g t c t t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c

780 UbC promoter c t g g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g g a c c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c

840 UbC promoter t c g t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g a g c a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c

900 UbC promoter g g a g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t c c t c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a

960 UbC promoter g g g g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g c c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c

1020 UbC promoter g c c t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g c g g a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c

1080 UbC promoter T G A A G C T C C hUBCpro-F t g a g g c g t c a g t t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c a c t c c g c a g t c a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g

1140 UbC promoter G G T T T T G A A C T hUBCpro-F g g t t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t c c a a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a

1200 UbC promoter t t g a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g a a c t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c

XbaI 1260 UbC promoter (in frame with WELQut site) *IRATKPKKTLRLKPQLDVR *IRATKPKKTLRLKPQLDVR c t a a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c g a a g c t t g g g c t g c a g g t c g a c t c t g a t t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g c t t c g a a c c c g a c g t c c a g c t g a g a

1320 (in frame with WELQut site) SGGHDPPHGREQEGDEEQSR Kozak sequence MVWRVPPFLLPILFLAS SGGHDPPHGREQEGDEEQSR a g a g c c a c c a t g g t c t g g c g g g t g c c c c c t t t c t t g c t c c c c a t c c t c t t c t t g g c t t c t t c t c g g t g g t a c c a g a c c g c c c a c g g g g g a a a g a a c g a g g g g t a g g a g a a g a a c c g a a g a

1380 (in frame with WELQut site) MHARRHVQRQQGVQPERVGL HVGAAVDLTLLANLRLTDPQ MHARRHVQRQQGVQPERVGL c a t g t g g g c g c g g c g g t g g a c c t g a c g c t g c t g g c c a a c c t g c g g c t c a c g g a c c c c c a g g t a c a c c c g c g c c g c c a c c t g g a c t g c g a c g a c c g g t t g g a c g c c g a g t g c c t g g g g g t c

1440 (in frame with WELQut site) AEEQSAHRPLGPRPPARVGP RFFLTCVSGEAGAGRGSDAW AEEQSAHRPLGPRPPARVGP c g c t t c t t c c t g a c t t g c g t g t c t g g g g a g g c c g g g g c g g g g a g g g g c t c g g a c g c c t g g g c g a a g a a g g a c t g a a c g c a c a g a c c c c t c c g g c c c c g c c c c t c c c c g a g c c t g c g g a c c

FspAI 1500 (in frame with WELQut site) ARGQQQLLVVTDHAGRGPWG GPPLLLEKDDRIVRTPPGPP ARGQQQLLVVTDHAGRGPWG g g c c c g c c c c t g c t g c t g g a g a a g g a c g a c c g t a t c g t g c g c a c c c c g c c c g g g c c a c c c c c g g g c g g g g a c g a c g a c c t c t t c c t g c t g g c a t a g c a c g c g t g g g g c g g g c c c g g t g g g

1560 (in frame with WELQut site) QAQRAVTRVLDRKAAEGLGR LRLARNGSHQVTLRGFSKPS QAQRAVTRVLDRKAAEGLGR c t g c g c c t g g c g c g c a a c g g t t c g c a c c a g g t c a c g c t t c g c g g c t t c t c c a a g c c c t c g g a c g c g g a c c g c g c g t t g c c a a g c g t g g t c c a g t g c g a a g c g c c g a a g a g g t t c g g g a g c

1620 (in frame with WELQut site) VEHADEGAHATSPRPARADD DLVGVFSCVGGAGARRTRVI VEHADEGAHATSPRPARADD g a c c t c g t g g g c g t c t t c t c c t g c g t g g g c g g t g c t g g g g c g c g g c g c a c g c g c g t c a t c c t g g a g c a c c c g c a g a a g a g g a c g c a c c c g c c a c g a c c c c g c g c c g c g t g c g c g c a g t a g

Tth111IPflFI 1680 (in frame with WELQut site) VHVVARSGVQKWVLDCVSHV YVHNSPGAHLLPDKVTHTVN VHVVARSGVQKWVLDCVSHV t a c g t g c a c a a c a g c c c t g g a g c c c a c c t g c t t c c a g a c a a g g t c a c a c a c a c t g t g a a c a t g c a c g t g t t g t c g g g a c c t c g g g t g g a c g a a g g t c t g t t c c a g t g t g t g t g a c a c t t g

1740 (in frame with WELQut site) FTVGSYKRCTHVLLLLCVHD KGDTAVLSARVHKEKQTDVI FTVGSYKRCTHVLLLLCVHD a a a g g t g a c a c c g c t g t a c t t t c t g c a c g t g t g c a c a a g g a g a a g c a g a c a g a c g t g a t c t t t c c a c t g t g g c g a c a t g a a a g a c g t g c a c a c g t g t t c c t c t t c g t c t g t c t g c a c t a g

1800 (in frame with WELQut site) PLAVSGVEVGQVPMFGLIPP WKSNGSYFYTLDWHEAQDGR MKPRMGG PLAVSGVEVGQVPM t g g a a g a g c a a c g g a t c c t a c t t c t a c a c c c t g g a c t g g c a t g a a g c c c a g g a t g g g c g g a c c t t c t c g t t g c c t a g g a t g a a g a t g t g g g a c c t g a c c g t a c t t c g g g t c c t a c c c g c c

1860 EQ 1 QLEW WELQut site FLLQLPNVQPPSSGIYSATY SCCSSQMCSHHRAASTVPLT t t c c t g c t g c a g c t c c c a a a t g t g c a g c c a c c a t c g a g c g g c a t c t a c a g t g c c a c t t a c a a g g a c g a c g t c g a g g g t t t a c a c g t c g g t g g t a g c t c g c c g t a g a t g t c a c g g t g a a t g

1920 LEASPLGSAFFRLIVRGCGA WKPAPWAAPSFGSSCGVVGL c t g g a a g c c a g c c c c c t g g g c a g c g c c t t c t t t c g g c t c a t c g t g c g g g g t t g t g g g g c t g a c c t t c g g t c g g g g g a c c c g t c g c g g a a g a a a g c c g a g t a g c a c g c c c c a a c a c c c c g a

1980 GRWGPGCTKECPGCLHGGVC GAGGQAVPRSAQVAYMEVSA g g g c g c t g g g g g c c a g g c t g t a c c a a g g a g t g c c c a g g t t g c c t a c a t g g a g g t g t c t g c c c c g c g a c c c c c g g t c c g a c a t g g t t c c t c a c g g g t c c a a c g g a t g t a c c t c c a c a g a c g

2040 HDHDGECVCPPGFTGTRCEQ TTMTANVYAPLASLAPAVNR c a c g a c c a t g a c g g c g a a t g t g t a t g c c c c c c t g g c t t c a c t g g c a c c c g c t g t g a a c a g g t g c t g g t a c t g c c g c t t a c a c a t a c g g g g g g a c c g a a g t g a c c g t g g g c g a c a c t t g t c

2100 ACREGRFGQSCQEQCPGISG PAERAVLGRAARSSAQAYQA g c c t g c a g a g a g g g c c g t t t t g g g c a g a g c t g c c a g g a g c a g t g c c c a g g c a t a t c a g g c c g g a c g t c t c t c c c g g c a a a a c c c g t c t c g a c g g t c c t c g t c a c g g g t c c g t a t a g t c c g

2160 CRGLTFCLPDPYGCSCGSGW AGASPSASQTPMAALVDLAG t g c c g g g g c c t c a c c t t c t g c c t c c c a g a c c c c t a t g g c t g c t c t t g t g g a t c t g g c t g g a c g g c c c c g g a g t g g a a g a c g g a g g g t c t g g g g a t a c c g a c g a g a a c a c c t a g a c c g a c c

2220 RGSQCQEACAPGHFGADCRL EEASAKKLVPLVILGLIADS a g a g g a a g c c a g t g c c a a g a a g c t t g t g c c c c t g g t c a t t t t g g g g c t g a t t g c c g a c t c t c t c c t t c g g t c a c g g t t c t t c g a a c a c g g g g a c c a g t a a a a c c c c g a c t a a c g g c t g a g

AgeI 2280 QCQCQNGGTCDRFSGCVCPS SASVRMVALVTGSVVVSAPL *FPPVQSRNLPQTQGE c a g t g c c a g t g t c a g a a t g g t g g c a c t t g t g a c c g g t t c a g t g g t t g t g t c t g c c c c t c t g t c a c g g t c a c a g t c t t a c c a c c g t g a a c a c t g g c c a a g t c a c c a a c a c a g a c g g g g a g a

2340 GWHGVHCEKSDRIPQILNMA GGMECTVRSQTGSPRSSTWP PHCPTCQSFDSRIGWIRLMA g g g t g g c a t g g a g t g c a c t g t g a g a a g t c a g a c c g g a t c c c c c a g a t c c t c a a c a t g g c c c c c a c c g t a c c t c a c g t g a c a c t c t t c a g t c t g g c c t a g g g g g t c t a g g a g t t g t a c c g g

2400 SELEFNLETMPRINCAAAGN QNWSST* ESSSNLKSVIGRILQAAAPF t c a g a a c t g g a g t t c a a c t t a g a g a c g a t g c c c c g g a t c a a c t g t g c a g c t g c a g g g a a c a g t c t t g a c c t c a a g t t g a a t c t c t g c t a c g g g g c c t a g t t g a c a c g t c g a c g t c c c t t g

2460 PFPVRGSIELRKPDGTVLLS GKGTRPLMSSRLGSPVTSRD c c c t t c c c c g t g c g g g g c a g c a t a g a g c t a c g c a a g c c a g a c g g c a c t g t g c t c c t g t c c g g g a a g g g g c a c g c c c c g t c g t a t c t c g a t g c g t t c g g t c t g c c g t g a c a c g a g g a c a g g

2520 TKAIVEPEKTTAEFEVPRLV VLAMTSGSFVVASNSTGRKT a c c a a g g c c a t t g t g g a g c c a g a g a a g a c c a c a g c t g a g t t c g a g g t g c c c c g c t t g g t t t g g t t c c g g t a a c a c c t c g g t c t c t t c t g g t g t c g a c t c a a g c t c c a c g g g g c g a a c c a a

2580 LADSGFWECRVSTSGGQDSR RASLPNQSHRTDVDPPWSLR c t t g c g g a c a g t g g g t t c t g g g a g t g c c g t g t g t c c a c a t c t g g c g g c c a a g a c a g c c g g g a a c g c c t g t c a c c c a a g a c c c t c a c g g c a c a c a g g t g t a g a c c g c c g g t t c t g t c g g c c

2640 RFKVNVKVPPVPLAAPRLLT RKLTLTFTGGTGRAAGRSRV c g c t t c a a g g t c a a t g t g a a a g t g c c c c c c g t g c c c c t g g c t g c a c c t c g g c t c c t g a c c g c g a a g t t c c a g t t a c a c t t t c a c g g g g g g c a c g g g g a c c g a c g t g g a g c c g a g g a c t g g

2700 KQSRQLVVSPLVSFSGDGPI LCLRWSTTEGSTENEPSPGM a a g c a g a g c c g c c a g c t t g t g g t c t c c c c g c t g g t c t c g t t c t c t g g g g a t g g a c c c a t c t t c g t c t c g g c g g t c g a a c a c c a g a g g g g c g a c c a g a g c a a g a g a c c c c t a c c t g g g t a g

2760 STVRLHYRPQDSTMDWSTIV t c c a c t g t c c g c c t g c a c t a c c g g c c c c a g g a c a g t a c c a t g g a c t g g t c g a c c a t t g t g a g g t g a c a g g c g g a c g t g a t g g c c g g g g t c c t g t c a t g g t a c c t g a c c a g c t g g t a a c a c

2820 VDPSENVTLMNLRPKTGYSV g t g g a c c c c a g t g a g a a c g t g a c g t t a a t g a a c c t g a g g c c a a a g a c a g g a t a c a g t g t t c a c c t g g g g t c a c t c t t g c a c t g c a a t t a c t t g g a c t c c g g t t t c t g t c c t a t g t c a c a a

BlpI 2880 RVQLSRPGEGGEGAWGPPTL c g t g t g c a g c t g a g c c g g c c a g g g g a a g g a g g a g a g g g g g c c t g g g g g c c t c c c a c c c t c g c a c a c g t c g a c t c g g c c g g t c c c c t t c c t c c t c t c c c c c g g a c c c c c g g a g g g t g g g a g

2940 MTTDCPEPLLQPWLEGWHVE a t g a c c a c a g a c t g t c c t g a g c c t t t g t t g c a g c c g t g g t t g g a g g g c t g g c a t g t g g a a t a c t g g t g t c t g a c a g g a c t c g g a a a c a a c g t c g g c a c c a a c c t c c c g a c c g t a c a c c t t

3000 GTDRLRVSWSLPLVPGPLVG g g c a c t g a c c g g c t g c g a g t g a g c t g g t c c t t g c c c t t g g t g c c c g g g c c a c t g g t g g g c c c g t g a c t g g c c g a c g c t c a c t c g a c c a g g a a c g g g a a c c a c g g g c c c g g t g a c c a c c c g

3060 DGFLLRLWDGTRGQERRENV g a c g g t t t c c t g c t g c g c c t g t g g g a c g g g a c a c g g g g g c a g g a g c g g c g g g a g a a c g t c c t g c c a a a g g a c g a c g c g g a c a c c c t g c c c t g t g c c c c c g t c c t c g c c g c c c t c t t g c a g

3120 SSPQARTALLTGLTPGTHYQ t c a t c c c c c c a g g c c c g c a c t g c c c t c c t g a c g g g a c t c a c g c c t g g c a c c c a c t a c c a g a g t a g g g g g g t c c g g g c g t g a c g g g a g g a c t g c c c t g a g t g c g g a c c g t g g g t g a t g g t c

3180 LDVQLYHCTLLGPASPPAHV c t g g a t g t g c a g c t c t a c c a c t g c a c c c t c c t g g g c c c g g c c t c g c c c c c t g c a c a c g t g g a c c t a c a c g t c g a g a t g g t g a c g t g g g a g g a c c c g g g c c g g a g c g g g g g a c g t g t g c a c

3240 LLPPSGPPAPRHLHAQALSD c t t c t g c c c c c c a g t g g g c c t c c a g c c c c c c g a c a c c t c c a c g c c c a g g c c c t c t c a g a c g a a g a c g g g g g g t c a c c c g g a g g t c g g g g g g c t g t g g a g g t g c g g g t c c g g g a g a g t c t g

3300 SEIQLTWKHPEALPGPISKY t c c g a g a t c c a g c t g a c a t g g a a g c a c c c g g a g g c t c t g c c t g g g c c a a t a t c c a a g t a c a g g c t c t a g g t c g a c t g t a c c t t c g t g g g c c t c c g a g a c g g a c c c g g t t a t a g g t t c a t g

3360 VVEVQVAGGAGDPLWIDVDR g t t g t g g a g g t g c a g g t g g c t g g g g g t g c a g g a g a c c c a c t g t g g a t a g a c g t g g a c a g g c a a c a c c t c c a c g t c c a c c g a c c c c c a c g t c c t c t g g g t g a c a c c t a t c t g c a c c t g t c c

3420 PEETSTIIRGLNASTRYLFR c c t g a g g a g a c a a g c a c c a t c a t c c g t g g c c t c a a c g c c a g c a c g c g c t a c c t c t t c c g c g g a c t c c t c t g t t c g t g g t a g t a g g c a c c g g a g t t g c g g t c g t g c g c g a t g g a g a a g g c g

3480 MRASIQGLGDWSNTVEESTL a t g c g g g c c a g c a t t c a g g g g c t c g g g g a c t g g a g c a a c a c a g t a g a a g a g t c c a c c c t g t a c g c c c g g t c g t a a g t c c c c g a g c c c c t g a c c t c g t t g t g t c a t c t t c t c a g g t g g g a c

3540 GNGLQAEGPVQESRAAEEGL g g c a a c g g g c t g c a g g c t g a g g g c c c a g t c c a a g a g a g c c g g g c a g c t g a a g a g g g c c t g c c g t t g c c c g a c g t c c g a c t c c c g g g t c a g g t t c t c t c g g c c c g t c g a c t t c t c c c g g a c

3600 DQQLILAVVGSVSATCLTIL g a t c a g c a g c t g a t c c t g g c g g t g g t g g g c t c c g t g t c t g c c a c c t g c c t c a c c a t c c t g c t a g t c g t c g a c t a g g a c c g c c a c c a c c c g a g g c a c a g a c g g t g g a c g g a g t g g t a g g a c

3660 AALLTLVCIRRSCLHRRRTF g c c g c c c t t t t a a c c c t g g t g t g c a t c c g c a g a a g c t g c c t g c a t c g g a g a c g c a c c t t c c g g c g g g a a a a t t g g g a c c a c a c g t a g g c g t c t t c g a c g g a c g t a g c c t c t g c g t g g a a g

3720 TYQSGSGEETILQFSSGTLT a c c t a c c a g t c a g g c t c g g g c g a g g a g a c c a t c c t g c a g t t c a g c t c a g g g a c c t t g a c a t g g a t g g t c a g t c c g a g c c c g c t c c t c t g g t a g g a c g t c a a g t c g a g t c c c t g g a a c t g t

3780 LTRRPKLQPEPLSYPVLEWE c t t a c c c g g c g g c c a a a a c t g c a g c c c g a g c c c c t g a g c t a c c c a g t g c t a g a g t g g g a g g a a t g g g c c g c c g g t t t t g a c g t c g g g c t c g g g g a c t c g a t g g g t c a c g a t c t c a c c c t c

3840 DITFEDLIGEGNFGQVIRAM g a c a t c a c c t t t g a g g a c c t c a t c g g g g a g g g g a a c t t c g g c c a g g t c a t c c g g g c c a t g c t g t a g t g g a a a c t c c t g g a g t a g c c c c t c c c c t t g a a g c c g g t c c a g t a g g c c c g g t a c

3900 IKKDGLKMNAAIKMLKEYAS a t c a a g a a g g a c g g g c t g a a g a t g a a c g c a g c c a t c a a a a t g c t g a a a g a g t a t g c c t c t t a g t t c t t c c t g c c c g a c t t c t a c t t g c g t c g g t a g t t t t a c g a c t t t c t c a t a c g g a g a

3960 ENDHRDFAGELEVLCKLGHH g a a a a t g a c c a t c g t g a c t t t g c g g g a g a a c t g g a a g t t c t g t g c a a a t t g g g g c a t c a c c t t t t a c t g g t a g c a c t g a a a c g c c c t c t t g a c c t t c a a g a c a c g t t t a a c c c c g t a g t g

4020 PNIINLLGACKNRGYLYIAI c c c a a c a t c a t c a a c c t c c t g g g g g c c t g t a a g a a c c g a g g t t a c t t g t a t a t c g c t a t t g g g t t g t a g t a g t t g g a g g a c c c c c g g a c a t t c t t g g c t c c a a t g a a c a t a t a g c g a t a a

4080 EYAPYGNLLDFLRKSRVLET g a a t a t g c c c c c t a c g g g a a c c t g c t a g a t t t t c t g c g g a a a a g c c g g g t c c t a g a g a c t c t t a t a c g g g g g a t g c c c t t g g a c g a t c t a a a a g a c g c c t t t t c g g c c c a g g a t c t c t g a

4140 DPAFAREHGTASTLSSRQLL g a c c c a g c t t t t g c t c g a g a g c a t g g g a c a g c c t c t a c c c t t a g c t c c c g g c a g c t g c t g c t g g g t c g a a a a c g a g c t c t c g t a c c c t g t c g g a g a t g g g a a t c g a g g g c c g t c g a c g a c

4200 RFASDAANGMQYLSEKQFIH c g t t t c g c c a g t g a t g c g g c c a a t g g c a t g c a g t a c c t g a g t g a g a a g c a g t t c a t c c a c g c a a a g c g g t c a c t a c g c c g g t t a c c g t a c g t c a t g g a c t c a c t c t t c g t c a a g t a g g t g

4260 RDLAARNVLVGENLASKIAD a g g g a c c t g g c t g c c c g g a a c g t g c t g g t c g g a g a g a a c c t a g c c t c c a a g a t t g c a g a c t c c c t g g a c c g a c g g g c c t t g c a c g a c c a g c c t c t c t t g g a t c g g a g g t t c t a a c g t c t g

4320 FGLSRGEEVYVKKTMGRLPV t t c g g c c t t t c t c g g g g a g a g g a g g t t t a t g t g a a g a a g a c g a t g g g g c g t c t c c c t g t g a a g c c g g a a a g a g c c c c t c t c c t c c a a a t a c a c t t c t t c t g c t a c c c c g c a g a g g g a c a c

4380 RWMAIESLNYSVYTTKSDVW c g c t g g a t g g c c a t t g a g t c c c t g a a c t a c a g t g t c t a t a c c a c c a a g a g t g a t g t c t g g g c g a c c t a c c g g t a a c t c a g g g a c t t g a t g t c a c a g a t a t g g t g g t t c t c a c t a c a g a c c

4440 SFGVLLWEIVSLGGTPYCGM t c c t t t g g a g t c c t t c t t t g g g a g a t a g t g a g c c t t g g a g g t a c a c c c t a c t g t g g c a t g a g g a a a c c t c a g g a a g a a a c c c t c t a t c a c t c g g a a c c t c c a t g t g g g a t g a c a c c g t a c

4500 TCAELYEKLPQGYRMEQPRN a c c t g t g c c g a g c t c t a t g a a a a g c t g c c c c a g g g c t a c c g c a t g g a g c a g c c t c g a a a c t g g a c a c g g c t c g a g a t a c t t t t c g a c g g g g t c c c g a t g g c g t a c c t c g t c g g a g c t t t g

4560 CDDEVYELMRQCWRDRPYER t g t g a c g a t g a a g t g t a c g a g c t g a t g c g t c a g t g c t g g c g g g a c c g t c c c t a t g a g c g a a c a c t g c t a c t t c a c a t g c t c g a c t a c g c a g t c a c g a c c g c c c t g g c a g g g a t a c t c g c t

4620 PPFAQIALQLGRMLEARKAY c c c c c c t t t g c c c a g a t t g c g c t a c a g c t a g g c c g c a t g c t g g a a g c c a g g a a g g c c t a t g g g g g g a a a c g g g t c t a a c g c g a t g t c g a t c c g g c g t a c g a c c t t c g g t c c t t c c g g a t a

4680 VNMSLFENFTYAGIDATAEE g t g a a c a t g t c g c t g t t t g a g a a c t t c a c t t a c g c g g g c a t t g a t g c c a c a g c t g a g g a g c a c t t g t a c a g c g a c a a a c t c t t g a a g t g a a t g c g c c c g t a a c t a c g g t g t c g a c t c c t c

EcoRIAscI 4740 1 5 DYKDDDDK * FLAG AGDYKDDDDK* g c c g g t g a c t a c a a a g a c g a t g a c g a c a a g t g a g g c g c g c c g a a t t c g a t a t c a a g c t t a c g g c c a c t g a t g t t t c t g c t a c t g c t g t t c a c t c c g c g c g g c t t a a g c t a t a g t t c g a a t

4800 WPRE A C WPRE-R t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c

4860 WPRE A A C G A G G A A A A T G C G A T A C WPRE-R MLLL MPLYHAIA t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a

4920 WPRE PVWLSFSPPCINPGCCLFMR SRMAFIFSSLYKSWLLSLYE c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c

4980 WPRE SCGPLSGNVAWCALCLLTQP ELWPVVRQRGVVCTVFADAT a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g

5040 WPRE PLVGALPPPVSSFPGLSLSP PTGWGIATTCQLLSGTFAFP c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g

5100 WPRE SLLPRRNSSPPALPAAGQGL LPIATAELIAACLARCWTGA t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g

5160 WPRE (in frame with Factor Xa site) *RGKRP GCWALTIPWCCRGNHRPFLG RLLGTDNSVVLSGKSSSFPW g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g

5220 WPRE (in frame with Factor Xa site) QEGTNGGPNQAPRGEAVDR R Factor Xa site CSPVLPPGFCAGRPSATSLR LLACVATWILRGTSFCYVPS t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c

5280 WPRE 1 GEI Factor Xa site PSIQRTFLPAACCRLCGLFR ALNPADLPSRGLLPALRPLP c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g

5340 WPRE KS primer C T A pBluescriptKS VFAFALRRVGSPFGPPPRID RLRLRPQTSRISLWAASPHR g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a

5400 KS primer T G G C A G C T G G A G C T pBluescriptKS TVDLET* YRRPRDLEKHGAITSSNTAA a c c g t c g a c c t c g a g a c c t a g a a a a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a t g g c a g c t g g a g c t c t g g a t c t t t t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t

5460 TNADCAWLEAQEEEEVGFPV c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a g g t t a c g a c t a a c a c g g a c c g a t c t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t

Acc65IKpnI 5520 TPQVPLRPMTYKAAVDLSHF c a c c t c a g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a

5580 LKEKGGLEGLIHSQRRQDIL t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c

5640 DLWIYHTQGYFPDWQNYTPG a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g

5700 PGIRYPLTFGWCYKLVPVEQ c a g g g a t c a g a t a t c c a c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g g t c c c t a g t c t a t a g g t g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c

5760 EKVEEANEGENTRLLHPVSL a g a a g g t a g a a g a a g c c a a t g a a g g a g a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c t c t t c c a t c t t c t t c g g t t a c t t c c t c t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g

5820 HGMDDPEREVLEWRFDSRLA a t g g g a t g g a t g a c c c g g a g a g a g a a g t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t a c c c t a c c t a c t g g g c c t c t c t c t t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a

BspEI 5880 5' LTR (truncated) FHHMARELHPDCTGSLWLDQ t t c a t c a c a t g g c c c g a g a g c t g c a t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a a a g t a g t g t a c c g g g c t c t c g a c g t a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t

5940 5' LTR (truncated) I* t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a

6000 5' LTR (truncated) t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a

PmeI 6060 5' LTR (truncated) c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t

6120 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a

6180 bGH poly(A) signal t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t

6240 bGH poly(A) signal t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c

6300 bGH poly(A) signal g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a

6360 f1 ori g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t

6420 f1 ori t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t

6480 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a

6540 f1 ori c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g

6600 f1 ori c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a

6660 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t

6720 f1 ori a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c

6780 f1 ori C C T pBABE 3' g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t

6840 SV40 promoter T A C A C A C A G T C A A T C C C A pBABE 3' a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t

6900 SV40 promoter g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t

6960 SV40 promoter g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g

7020 SV40 promoter SV40 ori c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a

7080 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c

7140 SV40 promoter SV40 ori g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a

7200 EM7 promoter t c g g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a g c c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t

7260 1 5 10 MAKLTSAVPVLT EM7 promoter BleoR a t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c t a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g

MauBI 7320 15 20 25 30 ARDVAGAVEFWTDRLGFSRD BleoR c g c g c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a g c g c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t

SgrAI 7380 35 40 45 50 FVEDDFAGVVRDDVTLFISA BleoR c t t c g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g a a g c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g

7440 55 60 65 70 VQDQVVPDNTLAWVWVRGLD BleoR g g t c c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c c a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t

7500 75 80 85 90 ELYAEWSEVVSTNFRDASGP BleoR c g a g c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g c t c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g

FseI 7560 95 100 105 110 AMTEIGEQPWGREFALRDPA BleoR g g c c a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c c g g t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g

7620 115 120 125 GNCVHFVAEEQD* BleoR c g g c a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a g c c g t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t

7680 t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c

7740 SV40 poly(A) signal g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a

7800 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a

7860 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c

BstZ17I 7920 M13 rev C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C M13/pUC Reverse t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c

7980 -10 -35 lac operator lac promoter T T T A A C A A T A G G C G A M13/pUC Reverse a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g

8040 CAP binding site c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a

8100 c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c

8160 G A G C G A G T G A C T G A G C G A L4440 c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a

8220 t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g

8280 a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t

8340 ori a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t

8400 ori T T T C T A T G G T C C G C A A A G G pBR322ori-F t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g

8460 ori G pBR322ori-F c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g

8520 ori c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c

8580 ori t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t

8640 ori c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g

8700 ori g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g

8760 ori a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c

8820 ori c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t

8880 ori a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t

8940 ori a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t

9000 c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a

9060 a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c

9120 285 280 275 270 *WHKILSAGIEAIQRNREDM AmpR *WHKILSAGIEAIQRNREDM t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a

AhdI 9180 265 260 255 250 TAQSGTTYIVVIRSPKGDPG AmpR TAQSGTTYIVVIRSPKGDPG a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g

9240 245 240 235 230 LAAIIGRSGREGAGSKDAIF AmpR LAAIIGRSGREGAGSKDAIF c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t

9300 225 220 215 210 WGAPLASRLLPGAVKDAEMW AmpR WGAPLASRLLPGAVKDAEMW c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t

9360 205 200 195 190 DILQQRSALTLLEGTLLKRL AmpR DILQQRSALTLLEGTLLKRL g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t

9420 185 180 175 170 TTAMAVPMTTDREDNPIAEN AmpR TTAMAVPMTTDREDNPIAEN c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a

9480 165 160 155 150 LEPEWRDLRTVHDGMNHLFA AmpR LEPEWRDLRTVHDGMNHLFA c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g

PvuI 9540 145 140 135 130 TLEKPGGITTLLLNAATNDS AmpR TLEKPGGITTLLLNAATNDS g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a

9600 125 120 115 110 MTIAASCLERVTMGDTLHKE AmpR MTIAASCLERVTMGDTLHKE c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g

ScaI 9660 105 100 95 90 TVPSYEVLDNQSYHIRRGLQ AmpR TVPSYEVLDNQSYHIRRGLQ t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c

9720 85 80 75 70 EQGADIRSLVAGCLLVKFTS AmpR A T A A T A C C G C G C C A C A T A G C Amp-R EQGADIRSLVAGCLLVKFTS c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a

9780 65 60 55 50 MMPFREEPRFSELIKGSNLD AmpR MMPFREEPRFSELIKGSNLD c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g

9840 45 40 35 30 LEIYGVRAGLQDEADKVKVL AmpR LEIYGVRAGLQDEADKVKVL c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c

9900 25 TEPH 20 15 10 signal sequence AFVPLCFAAFFPILAV AmpR TEPHAFVPLCFAAFFPILAV c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g

SspI 9960 5 1 signal sequence RFHQISM AmpR AmpR promoter RFHQISM a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c

10,020 AmpR promoter t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a

SgrDI 10,080 AmpR promoter t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a

10,140 A T G T T A G A C G A G A C T A C G G C pRS-marker c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c

10,200 c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t

10,260 a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c

MluI 10,320 CMV enhancer c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c

10,380 CMV enhancer t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a

10,440 CMV enhancer a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c

10,500 CMV enhancer a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a

NdeI 10,560 CMV enhancer t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a

10,620 CMV enhancer a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c

SnaBI 10,680 CMV enhancer CMV promoter g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c

10,740 CMV promoter t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g

10,800 CMV promoter c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t

10,860 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g

10,920 CMV promoter 5' LTR (truncated) t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c

10,980 5' LTR (truncated) g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a

11,040 5' LTR (truncated) g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g

11,100 5' LTR (truncated) c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t

11,160 HIV-1 Ψ g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t

11,220 HIV-1 Ψ g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c

11,280 HIV-1 Ψ a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t

11,340 a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g

11,400 a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t

11,460 c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t

11,520 t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c

NotI 11,580 g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c

11,640 MRDNWRSELY g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t

11,700 KYKVVKIEPLGVAPTKAKRR t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c

11,760 RRE VVQREKRAVGIGALFLGFLG a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c

11,820 RRE AAGSTMGAASMTLTVQARQL a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a

11,880 RRE LSGIVQQQNNLLRAIEAQQH a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t

11,940 RRE LLQLTVWGIKQLQARILAVE t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t

12,000 RRE RYLKDQQLLGIWGCSGKLIC a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c

AleI 12,060 TTAVPWNASWSNKSLEQIWN c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t

12,120 HTTWMEWDREINNYTSLIHS t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g

12,180 1 5 10 KNEQELLELD gp41 peptide LIEESQNQQEKNEQELLELD c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t

12,240 15 KWASL (in frame with gp41 peptide) WNWFNITNWLWYIKL gp41 peptide KWASLWNWFNITNWLWYIKL t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a

12,300 (in frame with gp41 peptide) FIMIVGGLVGLRIVFAVLSI FIMIVGGLVGLRIVFAVLSI a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a

12,360 (in frame with gp41 peptide) VNRVRQGYSPLSFQTHLPTP VNRVRQGYSPLSFQTHLPTP a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g

12,420 (in frame with gp41 peptide) RGPDRPEGIEEEGGERDRDR RGPDRPEGIEEEGGERDRDR g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c

12,480 (in frame with gp41 peptide) SIRLVNGSALRAPILQTNGS SIRLVNGSALRAPILQTNGS a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c

12,540 (in frame with gp41 peptide) IHPQF* cPPT/CTS IHPQF* t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t

12,600 cPPT/CTS t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t

3ʹ 5ʹ 12,652 cPPT/CTS t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g t t a a t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c a a t t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.