Addgene pcDNA3.1-m-Flt4 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA3.1-m-Flt4 Sequencing Result


Map View

NruI (10,411) MfeI (10,364) pRS-marker (10,247 .. 10,266) SgrDI (10,201) SspI (10,084) Amp-R (9830 .. 9849) ScaI (9760) PvuI (9650) AhdI (9280) pBR322ori-F (8528 .. 8547) L4440 (8277 .. 8294) CAP binding site lac promoter M13/pUC Reverse (8059 .. 8081) lac operator M13 Reverse (8046 .. 8062) M13 rev BstZ17I (8008) EBV-rev (7967 .. 7986) SV40pA-R (7913 .. 7932) BstBI (7718) Neo-F (7572 .. 7591) RsrII (7552) BssHII (7433) Neo-R (6962 .. 6981) SV40pro-F (6754 .. 6773) CsiI - SexAI * (6593) pBABE 3' (6507 .. 6527) F1ori-F (6367 .. 6388) DraIII (6303) F1ori-R (6157 .. 6176) BGH-rev (5794 .. 5811) SP6 (5757 .. 5774) MluI (10,431) SnaBI (356) CMV-F (535 .. 555) T7 (629 .. 648) T7 promoter HindIII (655) EcoRV (716) NotI (731) SbfI (921) PaeR7I - PspXI - XhoI (936) AleI * (1152) AfeI (1291) PaqCI (2106) BsiWI (2518) PshAI (2995) SP6 promoter pcDNA3.1-m-Flt4 10,437 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g

120 CMV enhancer c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t

180 CMV enhancer a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t

240 CMV enhancer c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g

300 CMV enhancer a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a

SnaBI 360 CMV enhancer g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a

420 CMV enhancer CMV promoter t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g

480 CMV promoter g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a

540 CMV promoter C G C A A A CMV-F g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t

600 CMV promoter T G G G C G G T A G G C G T G CMV-F t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c

HindIII 660 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t t t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a a

EcoRV 720 g g t a c c g a g c t c g g a t c c a c t a g t a a c g g c c g c c a g t g t g c t g g a a t t c t g c a g a t a t c c c c a t g g c t c g a g c c t a g g t g a t c a t t g c c g g c g g t c a c a c g a c c t t a a g a c g t c t a t a g g

NotI 780 MQPG a t c a c a c t g g c g g c c g c g c a g g t c c c a c c c g c g c c c a g g g g c c g g a g a t g c a g c c g g g c g t a g t g t g a c c g c c g g c g c g t c c a g g g t g g g c g c g g g t c c c c g g c c t c t a c g t c g g c c c g c

840 AALNLRLWLCLGLLQGLANG c t g c g c t g a a c c t g c g c c t g t g g c t c t g c c t c g g a c t c c t c c a a g g c c t g g c a a a t g g t t g a c g c g a c t t g g a c g c g g a c a c c g a g a c g g a g c c t g a g g a g g t t c c g g a c c g t t t a c c a a

900 YSMTPPTLNITEDSYVIDTG a c t c c a t g a c c c c t c c a a c c c t g a a c a t c a c a g a g g a t t c a t a t g t g a t t g a c a c c g g g g t g a g g t a c t g g g g a g g t t g g g a c t t g t a g t g t c t c c t a a g t a t a c a c t a a c t g t g g c c c c

PaeR7IPspXIXhoISbfI 960 DSLSISCRGQHPLEWTWPGA a c a g c c t a t c c a t a t c c t g c a g g g g a c a g c a c c c c c t c g a g t g g a c c t g g c c a g g g g c c c t g t c g g a t a g g t a t a g g a c g t c c c c t g t c g t g g g g g a g c t c a c c t g g a c c g g t c c c c g g g

1020 QEVLTTGGKDSEDTRVVHDC a g g a g g t a c t g a c c a c a g g t g g g a a g g a c a g t g a a g a c a c a c g g g t t g t g c a t g a c t g t g t c c t c c a t g a c t g g t g t c c a c c c t t c c t g t c a c t t c t g t g t g c c c a a c a c g t a c t g a c a c

1080 EGTEARPYCKVLLLAQTHAN a a g g c a c a g a a g c t a g g c c c t a c t g c a a g g t g c t a c t g c t g g c c c a g a c t c a c g c c a a c a t t c c g t g t c t t c g a t c c g g g a t g a c g t t c c a c g a t g a c g a c c g g g t c t g a g t g c g g t t g t

1140 NTGSYHCYYKYIKARIEGTT a c a c g g g c a g c t a c c a c t g c t a c t a c a a g t a c a t c a a g g c c c g g a t t g a g g g c a c c a c a g t g t g c c c g t c g a t g g t g a c g a t g a t g t t c a t g t a g t t c c g g g c c t a a c t c c c g t g g t g t c

AleI* 1200 AASTYVFVRDFKHPFINKPD c t g c c a g c a c c t a t g t g t t t g t a a g a g a c t t t a a a c a c c c t t t c a t c a a c a a a c c t g a c a g a c g g t c g t g g a t a c a c a a a c a t t c t c t g a a a t t t g t g g g a a a g t a g t t g t t t g g a c t g t

1260 TLLVNRKDSMWVPCLVSIPG c g c t c c t g g t c a a c a g g a a g g a c t c g a t g t g g g t g c c c t g c t t g g t g t c c a t t c c c g g c c g c g a g g a c c a g t t g t c c t t c c t g a g c t a c a c c c a c g g g a c g a a c c a c a g g t a a g g g c c g g

AfeI 1320 LNITLRSQSSALHPDGQEVL t c a a c a t c a c a c t g c g c t c g c a a a g c t c a g c g c t g c a c c c c g a c g g g c a g g a g g t g c t g t a g t t g t a g t g t g a c g c g a g c g t t t c g a g t c g c g a c g t g g g g c t g c c c g t c c t c c a c g a c a

1380 WDDRRGMRVPTQLLRDALYL g g g a t g a c c g c c g g g g c a t g c g g g t g c c c a c t c a a c t g t t g c g c g a t g c c c t g t a c c t g c c c c t a c t g g c g g c c c c g t a c g c c c a c g g g t g a g t t g a c a a c g c g c t a c g g g a c a t g g a c g

1440 QCETTWGDQNFLSNLFVVHI a g t g c g a g a c c a c c t g g g g t g a c c a g a a c t t c c t t t c c a a t c t c t t c g t c g t g c a c a t c a t c a c g c t c t g g t g g a c c c c a c t g g t c t t g a a g g a a a g g t t a g a g a a g c a g c a c g t g t a g t

1500 TGNELYDIQLYPKKSMELLV c a g g c a a t g a g c t c t a t g a c a t c c a g c t g t a c c c c a a g a a g t c a a t g g a g c t g t t g g t t g g t c c g t t a c t c g a g a t a c t g t a g g t c g a c a t g g g g t t c t t c a g t t a c c t c g a c a a c c a a c

1560 GEKLVLNCTVWAEFDSGVTF g a g a g a a g c t g g t t t t g a a c t g t a c a g t g t g g g c t g a g t t c g a c t c a g g t g t c a c c t t c g c t c t c t t c g a c c a a a a c t t g a c a t g t c a c a c c c g a c t c a a g c t g a g t c c a c a g t g g a a g c

1620 DWDYPGKQAERAKWVPERRS a c t g g g a t t a t c c a g g g a a g c a g g c a g a g c g g g c t a a g t g g g t a c c t g a g c g g c g t t c c c t g a c c c t a a t a g g t c c c t t c g t c c g t c t c g c c c g a t t c a c c c a t g g a c t c g c c g c a a g g g

1680 QQTHTELSSILTIHNVSQND a g c a g a c c c a c a c a g a a c t c t c c a g c a t c c t g a c c a t c c a c a a t g t c a g c c a g a a t g a c c t c g t c t g g g t g t g t c t t g a g a g g t c g t a g g a c t g g t a g g t g t t a c a g t c g g t c t t a c t g g

1740 LGPYVCEANNGIQRFRESTE t g g g c c c c t a t g t g t g t g a g g c c a a c a a t g g g a t t c a g c g g t t c c g g g a a a g c a c a g a g g a c c c g g g g a t a c a c a c a c t c c g g t t g t t a c c c t a a g t c g c c a a g g c c c t t t c g t g t c t c c

1800 VIVHEKPFISVEWLKGPVLE t c a t t g t g c a c g a a a a g c c c t t c a t c a g t g t c g a g t g g c t c a a a g g a c c t g t c c t g g a g g a g t a a c a c g t g c t t t t c g g g a a g t a g t c a c a g c t c a c c g a g t t t c c t g g a c a g g a c c t c c

1860 ATAGDELVKLPVKLAAYPPP c c a c a g c c g g t g a c g a g c t g g t g a a g c t a c c c g t g a a g c t g g c a g c t t a t c c c c c a c c g g g g t g t c g g c c a c t g c t c g a c c a c t t c g a t g g g c a c t t c g a c c g t c g a a t a g g g g g t g g c c

1920 EFQWYKDRKAVTGRHNPHAL a g t t c c a a t g g t a c a a g g a c a g a a a g g c a g t g a c t g g g c g c c a c a a t c c c c a t g c t c t g g t c a a g g t t a c c a t g t t c c t g t c t t t c c g t c a c t g a c c c g c g g t g t t a g g g g t a c g a g a c c

1980 VLKEVTEASAGVYTLALWNS t g c t c a a a g a g g t g a c c g a g g c c a g c g c a g g g g t c t a c a c t c t c g c c c t g t g g a a c t c t g a c g a g t t t c t c c a c t g g c t c c g g t c g c g t c c c c a g a t g t g a g a g c g g g a c a c c t t g a g a c

2040 AAGLRQNISLELVVNVPPHI c a g c t g g t c t g a g g c a a a a c a t c a g t c t g g a g c t g g t g g t g a a t g t g c c t c c t c a c a t c c g t c g a c c a g a c t c c g t t t t g t a g t c a g a c c t c g a c c a c c a c t t a c a c g g a g g a g t g t a g g

2100 HEKEASSPSIYSRHSRQTLT a c g a a a a g g a a g c c t c t t c a c c c a g c a t c t a c t c c c g c c a c a g t c g c c a g a c c c t c a c c t t g c t t t t c c t t c g g a g a a g t g g g t c g t a g a t g a g g g c g g t g t c a g c g g t c t g g g a g t g g a

PaqCI 2160 CTAYGVPQPLSVQWHWRPWT g c a c c g c c t a t g g a g t a c c c c a a c c c c t c a g t g t c c a g t g g c a c t g g a g g c c c t g g a c a c c g t g g c g g a t a c c t c a t g g g g t t g g g g a g t c a c a g g t c a c c g t g a c c t c c g g g a c c t g t g

2220 PCKTFAQRSLRRRQQRDGMP c c t g c a a g a c g t t t g c c c a g c g c a g c c t c c g g a g g c g g c a g c a g c g g g a t g g c a t g c c a c g g a c g t t c t g c a a a c g g g t c g c g t c g g a g g c c t c c g c c g t c g t c g c c c t a c c g t a c g g t g

2280 QCRDWKEVTTQDAVNPIESL a g t g c c g a g a c t g g a a g g a g g t g a c c a c t c a g g a t g c t g t g a a c c c c a t c g a g a g t c t g g t c a c g g c t c t g a c c t t c c t c c a c t g g t g a g t c c t a c g a c a c t t g g g g t a g c t c t c a g a c c

2340 DSWTEFVEGKNKTVSKLVIQ a c a g c t g g a c g g a g t t t g t g g a g g g g a a g a a t a a g a c g g t g a g c a a g c t g g t g a t c c a g g t g t c g a c c t g c c t c a a a c a c c t c c c c t t c t t a t t c t g c c a c t c g t t c g a c c a c t a g g t c c

2400 DANVSAMYKCVVVNKVGQDE a t g c c a a t g t g t c a g c c a t g t a c a a g t g t g t g g t c g t c a a c a a a g t g g g c c a g g a t g a g a t a c g g t t a c a c a g t c g g t a c a t g t t c a c a c a c c a g c a g t t g t t t c a c c c g g t c c t a c t c t

2460 RLIYFYVTTIPDGFSIESEP g a c t c a t c t a c t t c t a t g t g a c c a c c a t c c c g g a c g g t t t c a g t a t c g a g t c g g a g c c t t c t g a g t a g a t g a a g a t a c a c t g g t g g t a g g g c c t g c c a a a g t c a t a g c t c a g c c t c g g a a

BsiWI 2520 SEDPLEGQSVRLSCRADNYT c t g a g g a t c c c t t a g a a g g c c a g t c c g t g c g c c t c a g c t g c c g g g c g g a c a a c t a c a c g t g a c t c c t a g g g a a t c t t c c g g t c a g g c a c g c g g a g t c g a c g g c c c g c c t g t t g a t g t g c a

2580 YEHLRWYRLNLSTLHDAQGN a c g a a c a t c t g c g c t g g t a c c g g c t c a a c c t c t c c a c a c t g c a c g a t g c t c a a g g g a a c c t g c t t g t a g a c g c g a c c a t g g c c g a g t t g g a g a g g t g t g a c g t g c t a c g a g t t c c c t t g g

2640 PLLLDCKNVHLFATPLEANL * c c c t a t t g c t g g a c t g c a a a a a c g t g c a c c t g t t t g c c a c g c c c c t a g a g g c c a a c c t a g g g g a t a a c g a c c t g a c g t t t t t g c a c g t g g a c a a a c g g t g c g g g g a t c t c c g g t t g g a t c

2700 EEAEPGARHATLSLNIPRVA LLCLGPGAVGGETQIDGSHR a g g a g g c a g a g c c c g g g g c c c g c c a c g c c a c c c t c a g t t t g a a t a t c c c c c g a g t g g c g c t c c t c c g t c t c g g g c c c c g g g c g g t g c g g t g g g a g t c a a a c t t a t a g g g g g c t c a c c g c g

2760 PEDEGDYVCEVQDRRSQDKH GLVLTVVHTFHLIPAALVLV c c g a g g a c g a g g g t g a c t a c g t g t g t g a a g t g c a g g a t a g g c g c a g c c a g g a c a a g c a c t g g c t c c t g c t c c c a c t g a t g c a c a c a c t t c a c g t c c t a t c c g c g t c g g t c c t g t t c g t g a

2820 CHKKYLSVQALEAPRLTQNL AVLLVQGHLGQFSRPERLVQ g c c a c a a g a a g t a c c t g t c c g t g c a g g c c c t g g a a g c t c c t c g g c t c a c g c a g a a c t t g a c g g t g t t c t t c a t g g a c a g g c a c g t c c g g g a c c t t c g a g g a g c c g a g t g c g t c t t g a a c t

2880 TDLLVNVSDSLEMRCPVAGA GVEQHVHTVGQLHSARHSSR c c g a c c t c c t g g t g a a c g t g a g t g a c t c c c t g g a g a t g c g a t g c c c g g t g g c t g g a g c g c g g c t g g a g g a c c a c t t g c a c t c a c t g a g g g a c c t c t a c g c t a c g g g c c a c c g a c c t c g c g

2940 HVPSIVWYKDERLLEKESGI MHGTNHPVFIFPEQLFLRSD a t g t g c c c a g t a t t g t g t g g t a c a a a g a t g a a a g g c t c c t g g a g a a a g a g t c g g g a a t c g t a c a c g g g t c a t a a c a c a c c a t g t t t c t a c t t t c c g a g g a c c t c t t t c t c a g c c c t t a g c

PshAI 3000 DLADSNQRLSIQRVREEDAG VQCVGILPQADLAHALLVCT a c c t g g c a g a c t c c a a t c a g a g g c t g a g c a t c c a g c g c g t g c g c g a g g a g g a c g c a g g t c t g g a c c g t c t g a g g t t a g t c t c c g a c t c g t a g g t c g c g c a c g c g c t c c t c c t g c g t c c a g

3060 RYLCSVCNAKGCVNSSASVA TIQAAHAIGLAAYVGRGAHC g t t a t c t g t g c a g c g t g t g c a a t g c c a a g g g c t g c g t a a a c t c c t c t g c c a g c g t g g c a g c a a t a g a c a c g t c g c a c a c g t t a c g g t t c c c g a c g c a t t t g a g g a g a c g g t c g c a c c g t c

3120 VEGSEDKGSMEIVILIGTGV HFARFIFAAHLNHYENASAD t g g a a g g c t c t g a a g a t a a a g g c a g c a t g g a g a t t g t g a t a c t c a t t g g c a c t g g c g t c a a c c t t c c g a g a c t t c t a t t t c c g t c g t a c c t c t a a c a c t a t g a g t a a c c g t g a c c g c a g t

3180 IAVFFWVLLLLIFCNMKRPA DCNEEPDEEQEDETVHFPRG t c g c a g t t t t c t t c t g g g t c c t c c t c c t g c t c a t c t t c t g t a a c a t g a a a a g g c c t g c c c a g c g t c a a a a g a a g a c c c a g g a g g a g g a c g a g t a g a a g a c a t t g t a c t t t t c c g g a c g g g

3240 HADIKTGYLSIIMDPGEVPL M a t g c a g a c a t c a a g a c g g g c t a c c t g t c c a t c a t c a t g g a c c c c g g g g a g g t g c c t t t g g t a c g t c t g t a g t t c t g c c c g a t g g a c a g g t a g t a g t a c c t g g g g c c c c t c c a c g g a a a c c

3300 EEQCEYLSYDASQWEFPRER a g g a g c a g t g t g a a t a c c t g t c c t a t g a c g c c a g c c a g t g g g a g t t c c c c a g g g a a a g g t t c c t c g t c a c a c t t a t g g a c a g g a t a c t g c g g t c g g t c a c c c t c a a g g g g t c c c t t t c c a

3360 LHLGRVLGHGAFGKVVEASA t g c a c c t c g g g a g a g t c c t a g g c c a c g g g g c t t t t g g g a a g g t g g t g g a a g c c t c a g c t t a c g t g g a g c c c t c t c a g g a t c c g g t g c c c c g a a a a c c c t t c c a c c a c c t t c g g a g t c g a a

3420 FGINKGSSCDTVAVKMLKEG t t g g c a t c a a t a a a g g c a g c a g c t g t g a c a c c g t g g c t g t g a a g a t g c t g a a a g a g g g c g a a c c g t a g t t a t t t c c g t c g t c g a c a c t g t g g c a c c g a c a c t t c t a c g a c t t t c t c c c g c

3480 ATASEHRALMSELKILIHIG c t a c t g c c a g c g a g c a c c g t g c c c t g a t g t c g g a g c t c a a g a t c c t a a t t c a c a t c g g c a g a t g a c g g t c g c t c g t g g c a c g g g a c t a c a g c c t c g a g t t c t a g g a t t a a g t g t a g c c g t

3540 NHLNVVNLLGACTKPNGPLM a c c a t c t c a a c g t g g t c a a c c t c c t a g g g g c g t g c a c c a a g c c c a a c g g c c c t c t c a t g g t g g t a g a g t t g c a c c a g t t g g a g g a t c c c c g c a c g t g g t t c g g g t t g c c g g g a g a g t a c c

3600 VIVEFCKYGNLSNFLRVKRD t g a t c g t g g a g t t t t g c a a a t a c g g c a a c c t c t c c a a c t t c t t g c g t g t c a a g c g g g a c a a c t a g c a c c t c a a a a c g t t t a t g c c g t t g g a g a g g t t g a a g a a c g c a c a g t t c g c c c t g t

3660 TFNPYAEKSPEQRRRFRAMV c g t t c a a c c c c t a c g c g g a g a a g t c t c c g g a g c a a c g c a g g c g c t t c c g c g c c a t g g t a g g c a a g t t g g g g a t g c g c c t c t t c a g a g g c c t c g t t g c g t c c g c g a a g g c g c g g t a c c a t c

3720 EGAKADRRRPGSSDRALFTR a a g g c g c c a a a g c t g a t a g g a g g a g a c c t g g a a g c a g c g a c a g g g c c c t g t t c a c g c g g t t t c c g c g g t t t c g a c t a t c c t c c t c t g g a c c t t c g t c g c t g t c c c g g g a c a a g t g c g c c a

3780 FLMGKGSARRAPLVQEAEDL t c c t g a t g g g c a a a g g a a g t g c a c g g c g a g c c c c a c t t g t c c a a g a a g c t g a g g a c c t a t a g g a c t a c c c g t t t c c t t c a c g t g c c g c t c g g g g t g a a c a g g t t c t t c g a c t c c t g g a t a

3840 WLSPLTMEDLVCYSFQVARG g g c t g a g c c c a c t g a c c a t g g a a g a c c t t g t a t g c t a c a g c t t c c a a g t t g c c c g g g g a a c c g a c t c g g g t g a c t g g t a c c t t c t g g a a c a t a c g a t g t c g a a g g t t c a a c g g g c c c c t t

3900 MEFLASRKCIHRDLAARNIL t g g a g t t c c t g g c t t c c c g c a a g t g c a t t c a c a g a g a c c t g g c t g c t c g g a a c a t c c t a c a c c t c a a g g a c c g a a g g g c g t t c a c g t a a g t g t c t c t g g a c c g a c g a g c c t t g t a g g a t g

3960 LSESDIVKICDFGLARDIYK t g t c a g a a a g t g a c a t a g t g a a g a t c t g c g a c t t t g g c c t c g c t c g g g a c a t c t a c a a a g a c a g t c t t t c a c t g t a t c a c t t c t a g a c g c t g a a a c c g g a g c g a g c c c t g t a g a t g t t t c

4020 DPDYVRKGSARLPLKWMAPE a c c c c g a c t a t g t c c g a a a g g g c a g t g c c c g a c t t c c t c t g a a a t g g a t g g c c c c c g a g a t g g g g c t g a t a c a g g c t t t c c c g t c a c g g g c t g a a g g a g a c t t t a c c t a c c g g g g g c t c t

4080 SIFDKVYTTQSDVWSFGVLL g c a t c t t t g a t a a g g t g t a c a c c a c g c a g a g t g a t g t g t g g t c c t t c g g c g t g c t g c t g t c g t a g a a a c t a t t c c a c a t g t g g t g c g t c t c a c t a c a c a c c a g g a a g c c g c a c g a c g a c a

4140 WEIFSLGASPYPGVQINEEF g g g a g a t c t t c t c a t t g g g g g c c t c t c c a t a c c c t g g g g t a c a g a t c a a t g a g g a g t t c t c c c t c t a g a a g a g t a a c c c c c g g a g a g g t a t g g g a c c c c a t g t c t a g t t a c t c c t c a a g a

4200 CQRLKDGTRMRAPELATPAI g c c a g c g g c t g a a g g a t g g c a c t c g a a t g a g a g c c c c g g a a c t g g c c a c t c c t g c c a t a c c g g t c g c c g a c t t c c t a c c g t g a g c t t a c t c t c g g g g c c t t g a c c g g t g a g g a c g g t a t g

4260 RHIMQSCWSGDPKARPAFSD g c c a c a t c a t g c a g a g t t g c t g g t c t g g a g a c c c t a a a g c a a g a c c t g c t t t c t c t g a c c c g g t g t a g t a c g t c t c a a c g a c c a g a c c t c t g g g a t t t c g t t c t g g a c g a a a g a g a c t g g

4320 LVEILGDLLQGGGWQEEEEE t a g t g g a g a t c c t g g g g g a c c t g c t t c a g g g c g g a g g c t g g c a g g a g g a g g a a g a g g a g c a t c a c c t c t a g g a c c c c c t g g a c g a a g t c c c g c c t c c g a c c g t c c t c c t c c t t c t c c t c g

4380 RMALHSSQSSEEDGFMQAST g c a t g g c c c t g c a c a g c t c t c a g a g c t c c g a g g a g g a t g g c t t c a t g c a g g c a t c c a c c a c g t a c c g g g a c g t g t c g a g a g t c t c g a g g c t c c t c c t a c c g a a g t a c g t c c g t a g g t g g t

4440 TALHITEADADDSPPSMHCH c a g c t c t a c a t a t c a c c g a a g c a g a c g c t g a t g a t a g t c c a c c c a g c a t g c a t t g c c a c a g t c g a g a t g t a t a g t g g c t t c g t c t g c g a c t a c t a t c a g g t g g g t c g t a c g t a a c g g t g t

4500 SLAARYYNCVSFPGRLARGT g c c t g g c a g c c a g a t a t t a c a a c t g t g t g t c c t t t c c t g g g c g c c t g g c c a g a g g c a c t a c g g a c c g t c g g t c t a t a a t g t t g a c a c a c a g g a a a g g a c c c g c g g a c c g g t c t c c g t g a t

4560 KTPGSSRMKTFEELPMTPTT a g a c t c c a g g c t c t t c c a g g a t g a a g a c a t t t g a a g a a t t g c c c a t g a c c c c t a c a a c c t t c t g a g g t c c g a g a a g g t c c t a c t t c t g t a a a c t t c t t a a c g g g t a c t g g g g a t g t t g g a

4620 YKASMDNQTDSGMVLASEEF a c a a a g c c t c c a t g g a t a a c c a g a c a g a c a g c g g g a t g g t g c t g g c c t c a g a a g a g t t t g t g t t t c g g a g g t a c c t a t t g g t c t g t c t g t c g c c c t a c c a c g a c c g g a g t c t t c t c a a a c

4680 EELESRHRPEGSFSCKGPGQ a g g a g c t a g a a a g c a g g c a t a g a c c a g a a g g c a g c t t c a g c t g t a a a g g t c c t g g c c a g c t c c t c g a t c t t t c g t c c g t a t c t g g t c t t c c g t c g a a g t c g a c a t t t c c a g g a c c g g t c g

4740 HMDIPRGHPDPQGRRRRPTQ a c a t g g a t a t t c c c a g a g g a c a c c c t g a c c c c c a g g g g a g g c g g c g a c g g c c c a c t c a a g t g t a c c t a t a a g g g t c t c c t g t g g g a c t g g g g g t c c c c t c c g c c g c t g c c g g g t g a g t t c

4800 GAQGGKVFYNNEYGEVSQPC g g g c a c a a g g a g g c a a g g t g t t t t a t a a c a a c g a g t a t g g g g a g g t c t c c c a g c c a t g t a c c c g t g t t c c t c c g t t c c a c a a a a t a t t g t t g c t c a t a c c c c t c c a g a g g g t c g g t a c a t

4860 TEGDCCPSAGSTFFADSSY* c a g a a g g t g a c t g c t g c c c g t c t g c t g g c t c c a c c t t c t t c g c a g a c a g c a g c t a c t a a g g t c t t c c a c t g a c g a c g g g c a g a c g a c c g a g g t g g a a g a a g c g t c t g t c g t c g a t g a t t c

4920 g a a c a t g t g g c a a g g c c t c c t g c c c g t t g c t g c a a c c g g c t c t g a c a g c t g g g c t g g a g g c t t g t a c a c c g t t c c g g a g g a c g g g c a a c g a c g t t g g c c g a g a c t g t c g a c c c g a c c t c c

4980 c c c a g g t t g g g a a t t c a a c t c c t c t g a c t c c a g c c c t g g a g g t g t t t t a t g t c c c a c c c c g g g t c c a a c c c t t a a g t t g a g g a g a c t g a g g t c g g g a c c t c c a c a a a a t a c a g g g t g g g g

5040 c a c t a c a g c c t c c a t a t c c a g g a a g a g t c a a a c t c a g a c a a c g a g g g c t c c c t t g a a c t c g t g a t g t c g g a g g t a t a g g t c c t t c t c a g t t t g a g t c t g t t g c t c c c g a g g g a a c t t g a g

5100 t g t a c c g c t c c t g t a c c c t t c c c t g g g c a g c c a c c c t c a c c c g c a c a t t c g a t t g c c t t g a c a t g g c g a g g a c a t g g g a a g g g a c c c g t c g g t g g g a g t g g g c g t g t a a g c t a a c g g a a c

5160 t a g c t c a g c c t c c a c a g a t g c t g g c c c t g g a a t a a g c a c t c t g g c a g a g a a t t c t t c t c c a t c g a g t c g g a g g t g t c t a c g a c c g g g a c c t t a t t c g t g a g a c c g t c t c t t a a g a a g a g g

5220 a g c a c c t a t t t t c a t c g c t c t g g t g g g c g a c a g g g t t c t c a t a a g g a a t a c a t a t g t c t g t c g t g g a t a a a a g t a g c g a g a c c a c c c g c t g t c c c a a g a g t a t t c c t t a t g t a t a c a g a c

5280 a g a g t c a c a c g a t g a a t c a a g a a a g a g t t a g a c c a g a g c c a c c a t g t c c t g g c c c c a a a c t c t c a g t g t g c t a c t t a g t t c t t t c t c a a t c t g g t c t c g g t g g t a c a g g a c c g g g g t t t g

5340 c a t c c t c t c c a c a c a c a c a g t c c c t c t a c t t c c a a c t g c t t c c t g c t t c c t t c t c c t g g c g t a g g a g a g g t g t g t g t g t c a g g g a g a t g a a g g t t g a c g a a g g a c g a a g g a a g a g g a c c g

5400 c c c g g a g g g c t g a a g a a a g a t a t t t t c t a t c t c a a a g t c a c a g a g g a g g a g t g g a g g a g g g g g c c t c c c g a c t t c t t t c t a t a a a a g a t a g a g t t t c a g t g t c t c c t c c t c a c c t c c t c c

5460 g g g a g g a c t c c t c t g a g c a t c t a t c t a g c t c g t g g g t a g c t g t c t g t a c a c a a t g a c t a a c c c t c c t g a g g a g a c t c g t a g a t a g a t c g a g c a c c c a t c g a c a g a c a t g t g t t a c t g a t t

5520 t c a c a a t g a g g c a a c g c c t c t g c c c c t g a g t g t t g a g a a g a a c c g t t t t t t t g t t t g t g t a g t g t t a c t c c g t t g c g g a g a c g g g g a c t c a c a a c t c t t c t t g g c a a a a a a a c a a a c a c a

5580 g t g t g t g t g t g t g t t g g t t t t t t t t t t t t t t t a a t c a g a a g a t c g g g c g c t g t t g t a a a g c a c a c a c a c a c a c a a c c a a a a a a a a a a a a a a a t t a g t c t t c t a g c c c g c g a c a a c a t t t c

5640 a a t t t a c t c a t c a a a a g a t c a t c t g g a a a a g a a g c c c a a t c a a t a a c t g t t c t a g a t c c t t t a a a t g a g t a g t t t t c t a g t a g a c c t t t t c t t c g g g t t a g t t a t t g a c a a g a t c t a g g a

5700 a g t c c c c a a t a a c t g a a g g g c g g a c a t g a c a c a a c a t g c c a g t c a c c a c a g c t c t t c c c c t c a g g g g t t a t t g a c t t c c c g c c t g t a c t g t g t t g t a c g g t c a g t g g t g t c g a g a a g g g g

5760 SP6 promoter G A T A SP6 c g c c c c c t c t c t c g a a g g c t c c c a g g c t c c a c c g g c c t g g t c t a g a g g g c c c t a t t c t a t g c g g g g g a g a g a g c t t c c g a g g g t c c g a g g t g g c c g g a c c a g a t c t c c c g g g a t a a g a t a

5820 SP6 promoter bGH poly(A) signal T C A C A G T G G A T T T A SP6 G G A G C T G A C A C G G A A G A T BGH-rev a g t g t c a c c t a a a t g c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c t c a c a g t g g a t t t a c g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g

5880 bGH poly(A) signal c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c

5940 bGH poly(A) signal t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g

6000 bGH poly(A) signal t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c

6060 bGH poly(A) signal c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c

6120 f1 ori g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t

6180 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a

6240 f1 ori t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a

6300 f1 ori t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g

DraIII 6360 f1 ori g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a

6420 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a

6480 f1 ori t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g

6540 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g

SexAI*CsiI 6600 SV40 promoter a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c

6660 SV40 promoter t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g

6720 SV40 promoter SV40 ori a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g

6780 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g

6840 SV40 promoter SV40 ori t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t

6900 SV40 promoter a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t c t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t a g

6960 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR MIEQDGLHAGSPAAWVER g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c

7020 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LFGYDWAQQTIGCSDAAVFR g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c

7080 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR LSAQGRPVLFVKTDLSGALN g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t

7140 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR ELQDEAARLSWLATTGVPCA t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g

7200 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR AVLDVVTEAGRDWLLLGEVP a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g

7260 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR GQDLLSSHLAPAEKVSIMAD *RVKSRGLFYGDHSI g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t

7320 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR AMRRLHTLDPATCPFDHQAK CHPPQMRKIRSGAWEVVLRF t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t

7380 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR HRIERARTRMEAGLVDQDDL MADLSCTSPHFGTKDILIIQ a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a

BssHII 7440 160 165 170 175 DEEHQGLAPAELFARLKARM NeoR/KanR DEEHQGLAPAELFARLKARM VFLMLPERWGFQEGPELRAH g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a

7500 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR PDGEDLVVTHGDACLPNIMV GVALIEDHGMAIGAQRIDHH g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a

RsrII 7560 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR ENGRFSGFIDCGRLGVADRY FIAAKRSEDVTAPQTHRVAI g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t

7620 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F QDIALATRDIAEELGGEWAD LVYRQSGTINSFLKAAFPSV t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t

7680 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR RFLVLYGIAAPDSQRIAFYR AEEHKVTDGSGIRLADGEIA c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c

BstBI 7740 260 265 LLDEFF* NeoR/KanR LLDEFF* KKVLEESRSEPTRFSRGLSA c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c

7800 WGAM c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g

7860 g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c

7920 SV40 poly(A) signal C G T T A T C G SV40pA-R t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g

7980 SV40 poly(A) signal G T G G T T T G T C C A A A EBV-rev T A G T G T T T A A A G SV40pA-R a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t

BstZ17I 8040 SV40 poly(A) signal C T C A T C EBV-rev c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t

8100 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t

8160 -35 lac promoter CAP binding site c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t

8220 a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t

8280 G A G C L4440 t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c

8340 G A G T G A C T G A G C G A L4440 c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c

8400 g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t

8460 ori g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g

8520 ori c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t

8580 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c

8640 ori a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a

8700 ori c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a

8760 ori g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c

8820 ori t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g

8880 ori a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g

8940 ori a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t

9000 ori g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g

9060 ori a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c

9120 g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t

9180 a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a

9240 285 280 *WHKILSAGIE AmpR *WHKILSAGIE a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t

AhdI 9300 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c

9360 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t

9420 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a

9480 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a

9540 195 190 185 180 LEGTLLKRLTTAMAVPMTTD AmpR LEGTLLKRLTTAMAVPMTTD a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t

9600 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t

PvuI 9660 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t

9720 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a

ScaI 9780 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t

9840 95 90 85 80 SYHIRRGLQEQGADIRSLVA AmpR A T A A T A C C G C G Amp-R SYHIRRGLQEQGADIRSLVA g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c

9900 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g

9960 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t

10,020 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a

10,080 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a

SspI 10,140 AmpR promoter c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a

10,200 AmpR promoter a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g

SgrDI 10,260 A T G T T A G A C G A G A C pRS-marker g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c

10,320 T A C G G C pRS-marker a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a

MfeI 10,380 g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g

MluINruI 3ʹ 5ʹ 10,437 t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.