Addgene dre-cdk2 3'UTR sicheck2 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: dre-cdk2 3'UTR sicheck2


Map View

Acc65I (54) BglII (1) pBR322ori-F (6626 .. 6645) AhdI (5893) AseI (5718) Amp-R (5324 .. 5343) SspI (5088) BamHI (4970) MfeI (4877) HincII - HpaI (4868) SV40pA-R (4812 .. 4831) EBV-rev (4758 .. 4777) XbaI (4708) BstXI (4169) BtgZI (3669) AleI (3549) BsrGI (3541) KpnI (58) pBABE 3' (85 .. 105) SV40 promoter SfiI (357) StuI (403) pMT2-F (604 .. 623) NheI (684) BmtI (688) DraIII (882) PfoI * (974) EcoRV (1179) NruI (1355) ZraI (1389) PflFI - Tth111I (1390) AatII (1391) AsiSI (1640) PaeR7I - XhoI (1643) NotI (2193) BspQI - SapI (2393) BssHII (2497) TspMI - XmaI (2538) SmaI (2540) SacII (2555) PpuMI (2575) BstZ17I (2599) EcoRI (2905) BstBI (2909) AflIII - MluI (2969) PspOMI (3077) ApaI (3081) dre-cdk2 3'UTR sicheck2 6792 bp

Sequence View

Acc65IKpnIBglII 5ʹ 3ʹ 60 a g a t c t g c g c a g c a c c a t g g c c t g a a a t a a c c t c t g a a a g a g g a a c t t g g t t a g g t a c c t t c t a g a c g c g t c g t g g t a c c g g a c t t t a t t g g a g a c t t t c t c c t t g a a c c a a t c c a t g g a

120 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' t c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g a g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c

180 SV40 promoter SV40 enhancer g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c

240 SV40 promoter SV40 enhancer g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c

300 SV40 promoter SV40 enhancer SV40 ori c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g

SfiI 360 SV40 promoter SV40 ori a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c

StuI 420 SV40 promoter SV40 ori c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t

480 a g c t t g a t t c t t c t g a c a c a a c a g t c t c g a a c t t a a g c t g c a g a a g t t g g t c g t g a g g c a t c g a a c t a a g a a g a c t g t g t t g t c a g a g c t t g a a t t c g a c g t c t t c a a c c a g c a c t c c g t

540 chimeric intron c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a a c t g g g c t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t t g a c c c g a

600 chimeric intron t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c a t c c a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g a c t g t a g g

660 chimeric intron T T G C C T T T C T C T C C A C A G G T pMT2-F a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g t t c a a t t a c a g c t c t t a a g g c t a g a t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c a a g t t a a t g t c g a g a a t t c c g a t c t

BmtINheI 720 1 5 MASKVYDPE T7 promoter hRluc Kozak sequence MASKVYDPE g t a c t t a a t a c g a c t c a c t a t a g g c t a g c c a c c a t g g c t t c c a a g g t g t a c g a c c c c g a g c a t g a a t t a t g c t g a g t g a t a t c c g a t c g g t g g t a c c g a a g g t t c c a c a t g c t g g g g c t c

780 10 15 20 25 QRKRMITGPQWWARCKQMNV hRluc QRKRMITGPQWWARCKQMNV c a a c g c a a a c g c a t g a t c a c t g g g c c t c a g t g g t g g g c t c g c t g c a a g c a a a t g a a c g t g g t t g c g t t t g c g t a c t a g t g a c c c g g a g t c a c c a c c c g a g c g a c g t t c g t t t a c t t g c a c

840 30 35 40 45 LDSFINYYDSEKHAENAVIF hRluc LDSFINYYDSEKHAENAVIF c t g g a c t c c t t c a t c a a c t a c t a t g a t t c c g a g a a g c a c g c c g a g a a c g c c g t g a t t t t t g a c c t g a g g a a g t a g t t g a t g a t a c t a a g g c t c t t c g t g c g g c t c t t g c g g c a c t a a a a a

DraIII 900 50 55 60 65 LHGNAASSYLWRHVVPHIEP hRluc LHGNAASSYLWRHVVPHIEP c t g c a t g g t a a c g c t g c c t c c a g c t a c c t g t g g a g g c a c g t c g t g c c t c a c a t c g a g c c c g a c g t a c c a t t g c g a c g g a g g t c g a t g g a c a c c t c c g t g c a g c a c g g a g t g t a g c t c g g g

960 70 75 80 85 VARCIIPDLIGMGKSGKSGN hRluc VARCIIPDLIGMGKSGKSGN g t g g c t a g a t g c a t c a t c c c t g a t c t g a t c g g a a t g g g t a a g t c c g g c a a g a g c g g g a a t c a c c g a t c t a c g t a g t a g g g a c t a g a c t a g c c t t a c c c a t t c a g g c c g t t c t c g c c c t t a

PfoI* 1020 90 95 100 105 GSYRLLDHYKYLTAWFELLN hRluc GSYRLLDHYKYLTAWFELLN g g c t c a t a t c g c c t c c t g g a t c a c t a c a a g t a c c t c a c c g c t t g g t t c g a g c t g c t g a a c c c g a g t a t a g c g g a g g a c c t a g t g a t g t t c a t g g a g t g g c g a a c c a a g c t c g a c g a c t t g

1080 110 115 120 125 LPKKIIFVGHDWGACLAFHY hRluc LPKKIIFVGHDWGACLAFHY c t t c c a a a g a a a a t c a t c t t t g t g g g c c a c g a c t g g g g g g c t t g t c t g g c c t t t c a c t a c g a a g g t t t c t t t t a g t a g a a a c a c c c g g t g c t g a c c c c c c g a a c a g a c c g g a a a g t g a t g

1140 130 135 140 145 SYEHQDKIKAIVHAESVVDV hRluc SYEHQDKIKAIVHAESVVDV t c c t a c g a g c a c c a a g a c a a g a t c a a g g c c a t c g t c c a t g c t g a g a g t g t c g t g g a c g t g a g g a t g c t c g t g g t t c t g t t c t a g t t c c g g t a g c a g g t a c g a c t c t c a c a g c a c c t g c a c

EcoRV 1200 150 155 160 165 IESWDEWPDIEEDIALIKSE hRluc IESWDEWPDIEEDIALIKSE a t c g a g t c c t g g g a c g a g t g g c c t g a c a t c g a g g a g g a t a t c g c c c t g a t c a a g a g c g a a t a g c t c a g g a c c c t g c t c a c c g g a c t g t a g c t c c t c c t a t a g c g g g a c t a g t t c t c g c t t

1260 170 175 180 185 EGEKMVLENNFFVETMLPSK hRluc EGEKMVLENNFFVETMLPSK g a g g g c g a g a a a a t g g t g c t t g a g a a t a a c t t c t t c g t c g a g a c c a t g c t c c c a a g c a a g c t c c c g c t c t t t t a c c a c g a a c t c t t a t t g a a g a a g c a g c t c t g g t a c g a g g g t t c g t t c

1320 190 195 200 205 IMRKLEPEEFAAYLEPFKEK hRluc IMRKLEPEEFAAYLEPFKEK a t c a t g c g g a a a c t g g a g c c t g a g g a g t t c g c t g c c t a c c t g g a g c c a t t c a a g g a g a a g t a g t a c g c c t t t g a c c t c g g a c t c c t c a a g c g a c g g a t g g a c c t c g g t a a g t t c c t c t t c

NruI 1380 210 215 220 225 GEVRRPTLSWPREIPLVKGG hRluc GEVRRPTLSWPREIPLVKGG g g c g a g g t t a g a c g g c c t a c c c t c t c c t g g c c t c g c g a g a t c c c t c t c g t t a a g g g a g g c c c g c t c c a a t c t g c c g g a t g g g a g a g g a c c g g a g c g c t c t a g g g a g a g c a a t t c c c t c c g

ZraITth111IPflFIAatII 1440 230 235 240 245 KPDVVQIVRNYNAYLRASDD hRluc KPDVVQIVRNYNAYLRASDD a a g c c c g a c g t c g t c c a g a t t g t c c g c a a c t a c a a c g c c t a c c t t c g g g c c a g c g a c g a t t t c g g g c t g c a g c a g g t c t a a c a g g c g t t g a t g t t g c g g a t g g a a g c c c g g t c g c t g c t a

1500 250 255 260 265 LPKMFIESDPGFFSNAIVEG hRluc LPKMFIESDPGFFSNAIVEG c t g c c t a a g a t g t t c a t c g a g t c c g a c c c t g g g t t c t t t t c c a a c g c t a t t g t c g a g g g a g a c g g a t t c t a c a a g t a g c t c a g g c t g g g a c c c a a g a a a a g g t t g c g a t a a c a g c t c c c t

1560 270 275 280 285 AKKFPNTEFVKVKGLHFSQE hRluc AKKFPNTEFVKVKGLHFSQE g c t a a g a a g t t c c c t a a c a c c g a g t t c g t g a a g g t g a a g g g c c t c c a c t t c a g c c a g g a g c g a t t c t t c a a g g g a t t g t g g c t c a a g c a c t t c c a c t t c c c g g a g g t g a a g t c g g t c c t c

1620 290 295 300 305 DAPDEMGKYIKSFVERVLKN hRluc DAPDEMGKYIKSFVERVLKN g a c g c t c c a g a t g a a a t g g g t a a g t a c a t c a a g a g c t t c g t g g a g c g c g t g c t g a a g a a c c t g c g a g g t c t a c t t t a c c c a t t c a t g t a g t t c t c g a a g c a c c t c g c g c a c g a c t t c t t g

AsiSIPaeR7IXhoI 1680 310 EQ* hRluc EQ* g a g c a g t a a t t c t a g g c g a t c g c t c g a g a a c g a g a t c a a c t t t g g c a a g a c g g g a c t a a a c t c g t c a t t a a g a t c c g c t a g c g a g c t c t t g c t c t a g t t g a a a c c g t t c t g c c c t g a t t t

1740 a g t t t c a c a c a a a a c t t t t c t g a a a a t g a t t t c c c a g t t t g g a a a t c g t t c g g t t t t a t a t c a a a g t g t g t t t t g a a a a g a c t t t t a c t a a a g g g t c a a a c c t t t a g c a a g c c a a a a t a t

1800 a t c g a t t t g t a c g c t c g t t t t c c t a a a g t c a c t c t g g g t t t g t t t t a a t c t g a a g a t t a t t a g c t a a a c a t g c g a g c a a a a g g a t t t c a g t g a g a c c c a a a c a a a a t t a g a c t t c t a a t a

1860 c c a t t g c a a c c t g t c a c t g g a a a a a a a a c a g c t g t g c t a a t a g t a t g c g t g t g a t t a a a a g g t a a c g t t g g a c a g t g a c c t t t t t t t t g t c g a c a c g a t t a t c a t a c g c a c a c t a a t t t t

1920 t g a c t c g g a g g a t g t a a a t t t g c a t a a a g c a g a c g t g a t g t t a c t a a c a t a a c t t t a g t t a c t g a g c c t c c t a c a t t t a a a c g t a t t t c g t c t g c a c t a c a a t g a t t g t a t t g a a a t c a a

1980 c g g a g a t g a c a t t c c a t g g t t a t a t t g c t g a t a t a a c t t g t g a c g t t g t a t t g c t t a t a t g c c t c t a c t g t a a g g t a c c a a t a t a a c g a c t a t a t t g a a c a c t g c a a c a t a a c g a a t a t a

2040 c a c a c a a g t a c a t a t c g c a t t g a g c g c t t c g t c t a g c c t t a g a c t g a a t g a a a c t g t t t a g t g t g t t c a t g t a t a g c g t a a c t c g c g a a g c a g a t c g g a a t c t g a c t t a c t t t g a c a a a t

2100 g a c t c c c t g t a t c t t t t t g c t g c t t t a t t c c a a g a t t a t a t t a t t a t a a t t a a a g a t t t t c t g a g g g a c a t a g a a a a a c g a c g a a a t a a g g t t c t a a t a t a a t a a t a t t a a t t t c t a a a a

2160 a a a c c t a a t t t g c t t t t a a a g t t t c c t a a a a a t a g t t c t c t c t g c a t c t t c a t c c a t t a a t t t g g a t t a a a c g a a a a t t t c a a a g g a t t t t t a t c a a g a g a g a c g t a g a a g t a g g t a a t t

NotI 2220 poly(A) signal a c t c g t a t a a c a t t t g g t t t a t g t t g t t a c a g c g g c c g c t g g c c g c a a t a a a a t a t c t t t t g a g c a t a t t g t a a a c c a a a t a c a a c a a t g t c g c c g g c g a c c g g c g t t a t t t t a t a g a a a

2280 poly(A) signal HSV TK promoter a t t t t c a t t a c a t c t g t g t g t t g g t t t t t t g t g t g a g g a t c t a a a t g a g t c t t c g g a c c t t a a a a g t a a t g t a g a c a c a c a a c c a a a a a a c a c a c t c c t a g a t t t a c t c a g a a g c c t g g a

2340 HSV TK promoter c g c g g g g g c c g c t t a a g c g g t g g t t a g g g t t t g t c t g a c g c g g g g g g a g g g g g a a g g a a c g c g c c c c c g g c g a a t t c g c c a c c a a t c c c a a a c a g a c t g c g c c c c c c t c c c c c t t c c t t g

BspQISapI 2400 HSV TK promoter g a a a c a c t c t c a t t c g g a g g c g g c t c g g g g t t t g g t c t t g g t g g c c a c g g g c a c g c a g a a c t t t g t g a g a g t a a g c c t c c g c c g a g c c c c a a a c c a g a a c c a c c g g t g c c c g t g c g t c t t

2460 HSV TK promoter g a g c g c c g c g a t c c t c t t a a g c a c c c c c c c g c c c t c c g t g g a g g c g g g g g t t t g g t c g g c c t c g c g g c g c t a g g a g a a t t c g t g g g g g g g c g g g a g g c a c c t c c g c c c c c a a a c c a g c c g

BssHII 2520 HSV TK promoter g g g t g g t a a c t g g c g g g c c g c t g a c t c g g g c g g g t c g c g c g c c c c a g a g t g t g a c c t t t t c c c a c c a t t g a c c g c c c g g c g a c t g a g c c c g c c c a g c g c g c g g g g t c t c a c a c t g g a a a a

PpuMISacIITspMIXmaISmaI 2580 HSV TK promoter c g g t c t g c t c g c a g a c c c c c g g g c g g c g c c g c c g c g g c g g c g a c g g g c t c g c t g g g t c c t g c c a g a c g a g c g t c t g g g g g c c c g c c g c g g c g g c g c c g c c g c t g c c c g a g c g a c c c a g g a

BstZ17I 2640 HSV TK promoter MGTVYVDRLWSIRTTAVI a g g c t c c a t g g g g a c c g t a t a c g t g g a c a g g c t c t g g a g c a t c c g c a c g a c t g c g g t g a t t c c g a g g t a c c c c t g g c a t a t g c a c c t g t c c g a g a c c t c g t a g g c g t g c t g a c g c c a c t a

2700 HSV TK promoter LPETFCGTSRVTRLTRSVRW a t t a c c g g a g a c c t t c t g c g g g a c g a g c c g g g t c a c g c g g c t g a c g c g g a g c g t c c g t t g t a a t g g c c t c t g g a a g a c g c c c t g c t c g g c c c a g t g c g c c g a c t g c g c c t c g c a g g c a a c

2760 HSV TK promoter ATNTRTGHRYTILSPGGARD g g c g a c a a a c a c c a g g a c g g g g c a c a g g t a c a c t a t c t t g t c a c c c g g a g g c g c g a g g g a c c g c t g t t t g t g g t c c t g c c c c g t g t c c a t g t g a t a g a a c a g t g g g c c t c c g c g c t c c c t

2820 HSV TK promoter CRSFREWRSCFIPVARCSRL c t g c a g g a g c t t c a g g g a g t g g c g c a g c t g c t t c a t c c c c g t g g c c c g t t g c t c g c g t t t g a c g t c c t c g a a g t c c c t c a c c g c g t c g a c g a a g t a g g g g c a c c g g g c a a c g a g c g c a a a

2880 HSV TK promoter LAVSPEEIYLHVFSSMMTQT g c t g g c g g t g t c c c c g g a a g a a a t a t a t t t g c a t g t c t t t a g t t c t a t g a t g a c a c a a a c c g a c c g c c a c a g g g g c c t t c t t t a t a t a a a c g t a c a g a a a t c a a g a t a c t a c t g t g t t t g

EcoRIBstBI 2940 HSV TK promoter PPSVLSLANSNTQMQSGRRG c c c g c c c a g c g t c t t g t c a t t g g c g a a t t c g a a c a c g c a g a t g c a g t c g g g g c g g c g c g g g g g c g g g t c g c a g a a c a g t a a c c g c t t a a g c t t g t g c g t c t a c g t c a g c c c c g c c g c g c c

AflIIIMluI 3000 HSV TK promoter PRSTSHIKVTRVASNTERPC t c c c a g g t c c a c t t c g c a t a t t a a g g t g a c g c g t g t g g c c t c g a a c a c c g a g c g a c c c t g a g g g t c c a g g t g a a g c g t a t a a t t c c a c t g c g c a c a c c g g a g c t t g t g g c t c g c t g g g a c

3060 1 MAD HSV TK promoter luciferase Kozak sequence SDPLKSLAFRYCW* MAD *FSPMGTSNTFGGHGI c a g c g a c c c g c t t a a a a g c t t g g c a t t c c g g t a c t g t t g g t a a a g c c a c c a t g g c c g a t g g t c g c t g g g c g a a t t t t c g a a c c g t a a g g c c a t g a c a a c c a t t t c g g t g g t a c c g g c t a c

PspOMIApaI 3120 5 10 15 20 AKNIKKGPAPFYPLEDGTAG luciferase AKNIKKGPAPFYPLEDGTAG SLVNLLARSGEVRQLIAGSA c t a a g a a c a t t a a g a a g g g c c c t g c t c c c t t c t a c c c t c t g g a g g a t g g c a c c g c t g g c g g a t t c t t g t a a t t c t t c c c g g g a c g a g g g a a g a t g g g a g a c c t c c t a c c g t g g c g a c c g c

3180 25 30 35 40 EQLHKAMKRYALVPGTIAFT luciferase EQLHKAMKRYALVPGTIAFT LLQVLGHLPIGQHRAGNGEG a g c a g c t g c a c a a g g c c a t g a a g a g g t a t g c c c t g g t g c c t g g c a c c a t t g c c t t c a c c g t c g t c g a c g t g t t c c g g t a c t t c t c c a t a c g g g a c c a c g g a c c g t g g t a a c g g a a g t g g c

3240 45 50 55 60 DAHIEVDITYAEYFEMSVRL luciferase DAHIEVDITYAEYFEMSVRL IGVNLHVDGIGLVELHRHAQ a t g c c c a c a t t g a g g t g g a c a t c a c c t a t g c c g a g t a c t t c g a g a t g t c t g t g c g c c t g g t a c g g g t g t a a c t c c a c c t g t a g t g g a t a c g g c t c a t g a a g c t c t a c a g a c a c g c g g a c c

3300 65 70 75 80 AEAMKRYGLNTNHRIVVCSE luciferase AEAMKRYGLNTNHRIVVCSE GLGHLPVAQVGVVADHHARL c c g a g g c c a t g a a g a g g t a c g g c c t g a a c a c c a a c c a c c g c a t c g t g g t g t g c t c t g a g a g g c t c c g g t a c t t c t c c a t g c c g g a c t t g t g g t t g g t g g c g t a g c a c c a c a c g a g a c t c t

3360 85 90 95 100 NSLQFFMPVLGALFIGVAVA luciferase NSLQFFMPVLGALFIGVAVA VRQLEEHWHQAGQEDSHGHG a c t c t c t g c a g t t c t t c a t g c c a g t g c t g g g c g c c c t g t t c a t c g g a g t g g c c g t g g c c c t g a g a g a c g t c a a g a a g t a c g g t c a c g a c c c g c g g g a c a a g t a g c c t c a c c g g c a c c g g g

3420 105 110 115 120 PANDIYNERELLNSMGISQP luciferase PANDIYNERELLNSMGISQP RSVVNVVLALQQVAHANRLR c t g c t a a c g a c a t t t a c a a c g a g c g c g a g c t g c t g a a c a g c a t g g g c a t t t c t c a g c c t a g a c g a t t g c t g t a a a t g t t g c t c g c g c t c g a c g a c t t g t c g t a c c c g t a a a g a g t c g g a t

3480 125 130 135 140 TVVFVSKKGLQKILNVQKKL luciferase TVVFVSKKGLQKILNVQKKL GHHEHRLLAQLLDQVHLLLQ c c g t g g t g t t c g t g t c t a a g a a g g g c c t g c a g a a g a t c c t g a a c g t g c a g a a g a a g c t g c g g c a c c a c a a g c a c a g a t t c t t c c c g g a c g t c t t c t a g g a c t t g c a c g t c t t c t t c g a c g

3540 145 150 155 160 PIIQKIIIMDSKTDYQGFQS luciferase PIIQKIIIMDSKTDYQGFQS RDDLLDDDHVRLGVVLAELA c t a t c a t c c a g a a g a t c a t c a t c a t g g a c t c t a a g a c c g a c t a c c a g g g c t t c c a g a g c a g a t a g t a g g t c t t c t a g t a g t a g t a c c t g a g a t t c t g g c t g a t g g t c c c g a a g g t c t c g t

AleIBsrGI 3600 165 170 175 180 MYTFVTSHLPPGFNEYDFVP luciferase MYTFVTSHLPPGFNEYDFVP HVCEHCRMQRRAEVLVVEHW t g t a c a c a t t c g t g a c a t c t c a t c t g c c t c c t g g c t t c a a c g a g t a c g a c t t c g t g c c a g a c a t g t g t a a g c a c t g t a g a g t a g a c g g a g g a c c g a a g t t g c t c a t g c t g a a g c a c g g t c

3660 185 190 195 200 ESFDRDKTIALIMNSSGSTG luciferase ESFDRDKTIALIMNSSGSTG LREVPVFGNGQDHVARPRGA a g t c t t t c g a c a g g g a c a a a a c c a t t g c c c t g a t c a t g a a c a g c t c t g g g t c t a c c g g c c t c a g a a a g c t g t c c c t g t t t t g g t a a c g g g a c t a g t a c t t g t c g a g a c c c a g a t g g c c g g

BtgZI 3720 205 210 215 220 LPKGVALPHRTACVRFSHAR luciferase LPKGVALPHRTACVRFSHAR QRLAHGQRMAGGTHAERVGA t g c c t a a g g g c g t g g c c c t g c c t c a t c g c a c c g c c t g t g t g c g c t t c t c t c a c g c c c g c g a c g g a t t c c c g c a c c g g g a c g g a g t a g c g t g g c g g a c a c a c g c g a a g a g a g t g c g g g c g c

3780 225 230 235 240 DPIFGNQIIPDTAILSVVPF luciferase DPIFGNQIIPDTAILSVVPF VRNEAVLDDGVGSNQAHHWE a c c c t a t t t t c g g c a a c c a g a t c a t c c c c g a c a c c g c t a t t c t g a g c g t g g t g c c a t t c c t g g g a t a a a a g c c g t t g g t c t a g t a g g g g c t g t g g c g a t a a g a c t c g c a c c a c g g t a a g g

3840 245 250 255 260 HHGFGMFTTLGYLICGFRVV luciferase HHGFGMFTTLGYLICGFRVV VVAEAHEGGQAVQNAAKPHH a c c a c g g c t t c g g c a t g t t c a c c a c c c t g g g c t a c c t g a t t t g c g g c t t t c g g g t g g t g c t g g t g c c g a a g c c g t a c a a g t g g t g g g a c c c g a t g g a c t a a a c g c c g a a a g c c c a c c a c g

3900 265 270 275 280 LMYRFEEELFLRSLQDYKIQ luciferase LMYRFEEELFLRSLQDYKIQ QHVAELLLQEQAAQLVVFNL t g a t g t a c c g c t t c g a g g a g g a g c t g t t c c t g c g c a g c c t g c a a g a c t a c a a a a t t c a g t a c t a c a t g g c g a a g c t c c t c c t c g a c a a g g a c g c g t c g g a c g t t c t g a t g t t t t a a g t c a

3960 285 290 295 300 SALLVPTLFSFFAKSTLIDK luciferase SALLVPTLFSFFAKSTLIDK RGQQHWGQEAEESLAGQDVL c t g c c c t g c t g g t g c c a a c c c t g t t c a g c t t c t t c g c t a a g a g c a c c c t g a t c g a c a a g t g a c g g g a c g a c c a c g g t t g g g a c a a g t c g a a g a a g c g a t t c t c g t g g g a c t a g c t g t t c a

4020 305 310 315 320 YDLSNLHEIASGGAPLSKEV luciferase Shine-Dalgarno sequence YDLSNLHEIASGGAPLSKEV VVQRVQVLNGRAAGWQRLLH a c g a c c t g t c t a a c c t g c a c g a g a t t g c c t c t g g c g g c g c c c c a c t g t c t a a g g a g g t g g t g c t g g a c a g a t t g g a c g t g c t c t a a c g g a g a c c g c c g c g g g g t g a c a g a t t c c t c c a c c

4080 325 330 335 340 GEAVAKRFHLPGIRQGYGLT luciferase GEAVAKRFHLPGIRQGYGLT AFGHGLAKM g c g a a g c c g t g g c c a a g c g c t t t c a t c t g c c a g g c a t c c g c c a g g g c t a c g g c c t g a c c g c g c t t c g g c a c c g g t t c g c g a a a g t a g a c g g t c c g t a g g c g g t c c c g a t g c c g g a c t g g c

4140 345 350 355 360 ETTSAILITPEGDDKPGAVG luciferase ETTSAILITPEGDDKPGAVG a g a c a a c c a g c g c c a t t c t g a t t a c c c c a g a g g g c g a c g a c a a g c c t g g c g c c g t g g g c a t c t g t t g g t c g c g g t a a g a c t a a t g g g g t c t c c c g c t g c t g t t c g g a c c g c g g c a c c c g t

BstXI 4200 365 370 375 380 KVVPFFEAKVVDLDTGKTLG luciferase KVVPFFEAKVVDLDTGKTLG a g g t g g t g c c a t t c t t c g a g g c c a a g g t g g t g g a c c t g g a c a c c g g c a a g a c c c t g g g a g t c c a c c a c g g t a a g a a g c t c c g g t t c c a c c a c c t g g a c c t g t g g c c g t t c t g g g a c c c t c

4260 385 390 395 400 VNQRGELCVRGPMIMSGYVN luciferase VNQRGELCVRGPMIMSGYVN t g a a c c a g c g c g g c g a g c t g t g t g t g c g c g g c c c t a t g a t t a t g t c c g g c t a c g t g a a t a a c t t g g t c g c g c c g c t c g a c a c a c a c g c g c c g g g a t a c t a a t a c a g g c c g a t g c a c t t a t

4320 405 410 415 420 NPEATNALIDKDGWLHSGDI luciferase NPEATNALIDKDGWLHSGDI a c c c t g a g g c c a c a a a c g c c c t g a t c g a c a a g g a c g g c t g g c t g c a c t c t g g c g a c a t t g t g g g a c t c c g g t g t t t g c g g g a c t a g c t g t t c c t g c c g a c c g a c g t g a g a c c g c t g t a a c

4380 425 430 435 440 AYWDEDEHFFIVDRLKSLIK luciferase AYWDEDEHFFIVDRLKSLIK c c t a c t g g g a c g a g g a c g a g c a c t t c t t c a t c g t g g a c c g c c t g a a g t c t c t g a t c a a g t g g a t g a c c c t g c t c c t g c t c g t g a a g a a g t a g c a c c t g g c g g a c t t c a g a g a c t a g t t c a

4440 445 450 455 460 YKGYQVAPAELESILLQHPN luciferase YKGYQVAPAELESILLQHPN a c a a g g g c t a c c a g g t g g c c c c a g c c g a g c t g g a g t c t a t c c t g c t g c a g c a c c c t a a c a t g t t c c c g a t g g t c c a c c g g g g t c g g c t c g a c c t c a g a t a g g a c g a c g t c g t g g g a t t g t

4500 465 470 475 480 IFDAGVAGLPDDDAGELPAA luciferase IFDAGVAGLPDDDAGELPAA t t t t c g a c g c c g g a g t g g c c g g c c t g c c c g a c g a c g a t g c c g g c g a g c t g c c t g c c g c c g a a a a g c t g c g g c c t c a c c g g c c g g a c g g g c t g c t g c t a c g g c c g c t c g a c g g a c g g c g g c

4560 485 490 495 500 VVVLEHGKTMTEKEIVDYVA luciferase VVVLEHGKTMTEKEIVDYVA t c g t c g t g c t g g a a c a c g g c a a g a c c a t g a c c g a g a a g g a g a t c g t g g a c t a t g t g g c c a a g c a g c a c g a c c t t g t g c c g t t c t g g t a c t g g c t c t t c c t c t a g c a c c t g a t a c a c c g g t

4620 505 510 515 520 SQVTTAKKLRGGVVFVDEVP luciferase SQVTTAKKLRGGVVFVDEVP g c c a g g t g a c a a c c g c c a a g a a g c t g c g c g g c g g a g t g g t g t t c g t g g a c g a g g t g c c c a c g g t c c a c t g t t g g c g g t t c t t c g a c g c g c c g c c t c a c c a c a a g c a c c t g c t c c a c g g g t

4680 525 530 535 540 KGLTGKLDARKIREILIKAK luciferase KGLTGKLDARKIREILIKAK a g g g c c t g a c c g g c a a g c t g g a c g c c c g c a a g a t c c g c g a g a t c c t g a t c a a g g c t a a g a t c c c g g a c t g g c c g t t c g a c c t g c g g g c g t t c t a g g c g c t c t a g g a c t a g t t c c g a t t c t

XbaI 4740 545 550 KGGKIAV* luciferase KGGKIAV* a a g g c g g c a a g a t c g c c g t g t a a t a a t t c t a g a g t c g g g g c g g c c g g c c g c t t c g a g c a g t t c c g c c g t t c t a g c g g c a c a t t a t t a a g a t c t c a g c c c c g c c g g c c g g c g a a g c t c g t c

4800 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a

4860 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t

MfeIHincIIHpaI 4920 SV40 poly(A) signal a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c

BamHI 4980 a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c c a g g t g t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a g g t c c a c

5040 AmpR promoter g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t

SspI 5100 AmpR promoter a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t

5160 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFC AmpR promoter AmpR MSIQHFRVALIPFFAAFC a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g

5220 20 signal sequence LPVFA 25 30 35 HPETLVKVKDAEDQL AmpR LPVFAHPETLVKVKDAEDQL t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c

5280 40 45 50 55 GARVGYIELDLNSGKILESF AmpR GARVGYIELDLNSGKILESF g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g

5340 60 65 70 75 RPEERFPMMSTFKVLLCGAV AmpR C G A T A C A C C G C G C C A T A Amp-R RPEERFPMMSTFKVLLCGAV g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a

5400 80 85 90 95 LSRIDAGQEQLGRRIHYSQN AmpR A T A Amp-R LSRIDAGQEQLGRRIHYSQN t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c

5460 100 105 110 115 DLVEYSPVTEKHLTDGMTVR AmpR DLVEYSPVTEKHLTDGMTVR a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c

5520 120 125 130 135 ELCSAAITMSDNTAANLLLT AmpR ELCSAAITMSDNTAANLLLT a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t

5580 140 145 150 155 TIGGPKELTAFLHNMGDHVT AmpR TIGGPKELTAFLHNMGDHVT c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g

5640 160 165 170 175 RLDRWEPELNEAIPNDERDT AmpR RLDRWEPELNEAIPNDERDT g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t

5700 180 185 190 195 TMPVAMATTLRKLLTGELLT AmpR TMPVAMATTLRKLLTGELLT c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g

AseI 5760 200 205 210 215 LASRQQLIDWMEADKVAGPL AmpR LASRQQLIDWMEADKVAGPL t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g

5820 220 225 230 235 LRSALPAGWFIADKSGAGER AmpR LRSALPAGWFIADKSGAGER t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c

5880 240 245 250 255 GSRGIIAALGPDGKPSRIVV AmpR GSRGIIAALGPDGKPSRIVV g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t

AhdI 5940 260 265 270 275 IYTTGSQATMDERNRQIAEI AmpR IYTTGSQATMDERNRQIAEI t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c

6000 280 285 GASLIKHW* AmpR GASLIKHW* g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t

6060 t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g

6120 t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t

6180 ori a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t

6240 ori a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g

6300 ori c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a

6360 ori a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g

6420 ori t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g

6480 ori g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t

6540 ori g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c

6600 ori c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c

6660 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a

6720 ori t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a

6780 ori g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g

3ʹ 5ʹ 6792 a c a t g g c t c g a c t g t a c c g a g c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.