Addgene M3-PSCFP2 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: M3-PSCFP2 Sequencing Result

Map View

AflIII - PciI (6558) pBR322ori-F (6403 .. 6422) ApaLI (6244) BsaI (5629) TK-pA-R (5494 .. 5513) Neo-F (5176 .. 5195) RsrII (5156) PflFI - Tth111I (4758) FspI (4742) Neo-R (4566 .. 4585) SV40 promoter CsiI - SexAI * (4229) pBABE 3' (4143 .. 4163) F1ori-R (3881 .. 3900) DraIII (3756) F1ori-F (3669 .. 3690) EBV-rev (3495 .. 3514) SV40pA-R (3441 .. 3460) HpaI (3403) MfeI (3390) XbaI * (3294) AseI (44) CMV enhancer NdeI (271) CMV-F (556 .. 576) NheI (628) BmtI (632) AfeI (633) BglII (646) Eco53kI (655) SacI (657) EcoRI (666) BstEII (1007) BsmBI - Esp3I (1839) PaqCI (1925) SbfI (1936) PspOMI (2539) ApaI (2543) BamHI (2546) AgeI (2552) BstXI (2744) Bpu10I (2754) PpuMI (3191) PspXI (3225) NotI (3284) M3-PSCFP2 6579 bp

Sequence View

AseI 5ʹ 3ʹ 60 c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t

120 CMV enhancer c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c

180 CMV enhancer g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g

240 CMV enhancer c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t

NdeI 300 CMV enhancer c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t

360 CMV enhancer a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t

420 CMV enhancer CMV promoter c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a

480 CMV promoter a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c

540 CMV promoter a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t

600 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t

Eco53kISacIBglIINheIBmtIAfeI 660 CMV promoter g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g a c t c a g a t c t c g a g c t c a a c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c t g a g t c t a g a g c t c g a g t t

EcoRI 720 1 5 10 YPYDVPDYAYP 3xHA MYPYDVPDYAYP g c t t c g a a t t c c t t g g t a c c a c c a t g t a c c c a t a c g a t g t t c c a g a t t a c g c t t a c c c a t c g a a g c t t a a g g a a c c a t g g t g g t a c a t g g g t a t g c t a c a a g g t c t a a t g c g a a t g g g t a

780 15 20 25 YDVPDYAYPYDVPDYA (in frame with 3xHA) DTLH 3xHA YDVPDYAYPYDVPDYADTLH a c g a t g t t c c a g a t t a c g c t t a c c c a t a c g a t g t t c c a g a t t a c g c t g a t a c c t t g c a c a t g c t a c a a g g t c t a a t g c g a a t g g g t a t g c t a c a a g g t c t a a t g c g a c t a t g g a a c g t g t

840 (in frame with 3xHA) NNSTTSPLFPNISSSWIHSP NNSTTSPLFPNISSSWIHSP a t a a c a g t a c a a c c t c g c c t t t g t t t c c a a a c a t c a g c t c c t c c t g g a t a c a c a g c c c c t t a t t g t c a t g t t g g a g c g g a a a c a a a g g t t t g t a g t c g a g g a g g a c c t a t g t g t c g g g g a

900 (in frame with 3xHA) SDAGLPPGTVTHFGSYNVSR SDAGLPPGTVTHFGSYNVSR c c g a t g c a g g g c t g c c c c c g g g a a c c g t c a c t c a t t t c g g c a g c t a c a a t g t t t c t c g a g g g c t a c g t c c c g a c g g g g g c c c t t g g c a g t g a g t a a a g c c g t c g a t g t t a c a a a g a g c t c

960 (in frame with 3xHA) AAGNFSSPDGTTDDPLGGHT AAGNFSSPDGTTDDPLGGHT c a g c t g g c a a t t t c t c c t c t c c a g a c g g t a c c a c c g a t g a c c c t c t g g g a g g t c a t a c c g g t c g a c c g t t a a a g a g g a g a g g t c t g c c a t g g t g g c t a c t g g g a g a c c c t c c a g t a t g g c

BstEII 1020 (in frame with 3xHA) VWQVVFIAFLTGILALVTII VWQVVFIAFLTGILALVTII t c t g g c a a g t g g t c t t c a t c g c t t t c t t a a c g g g c a t c c t g g c c t t g g t g a c c a t c a t c g a g a c c g t t c a c c a g a a g t a g c g a a a g a a t t g c c c g t a g g a c c g g a a c c a c t g g t a g t a g c

1080 (in frame with 3xHA) GNILVIVSFKVNKQLKTVNN GNILVIVSFKVNKQLKTVNN g c a a c a t c c t g g t a a t t g t g t c a t t t a a g g t c a a c a a g c a g c t g a a g a c g g t c a a c a a c t c g t t g t a g g a c c a t t a a c a c a g t a a a t t c c a g t t g t t c g t c g a c t t c t g c c a g t t g t t g a

1140 (in frame with 3xHA) YFLLSLACADLIIGVISMNL YFLLSLACADLIIGVISMNL a c t t c c t c t t a a g c c t g g c c t g t g c c g a t c t g a t t a t c g g g g t c a t t t c a a t g a a t c t g t t g a a g g a g a a t t c g g a c c g g a c a c g g c t a g a c t a a t a g c c c c a g t a a a g t t a c t t a g a c a

1200 (in frame with 3xHA) FTTYIIMNRWALGNLACDLW FTTYIIMNRWALGNLACDLW t t a c g a c c t a c a t c a t c a t g a a t c g a t g g g c c t t a g g g a a c t t g g c c t g t g a c c t c t g g c a a t g c t g g a t g t a g t a g t a c t t a g c t a c c c g g a a t c c c t t g a a c c g g a c a c t g g a g a c c g

1260 (in frame with 3xHA) LAIDYVASNASVMNLLVISF LAIDYVASNASVMNLLVISF t t g c c a t t g a c t a c g t a g c c a g c a a t g c c t c t g t t a t g a a t c t t c t g g t c a t c a g c t t t g a a c g g t a a c t g a t g c a t c g g t c g t t a c g g a g a c a a t a c t t a g a a g a c c a g t a g t c g a a a c

1320 (in frame with 3xHA) DRYFSITRPLTYRAKRTTKR DRYFSITRPLTYRAKRTTKR a c a g a t a c t t t t c c a t c a c g a g g c c g c t c a c g t a c c g a g c c a a a c g a a c a a c a a a g a g a g t g t c t a t g a a a a g g t a g t g c t c c g g c g a g t g c a t g g c t c g g t t t g c t t g t t g t t t c t c t c

1380 (in frame with 3xHA) AGVMIGLAWVISFVLWAPAI AGVMIGLAWVISFVLWAPAI c c g g t g t g a t g a t c g g t c t g g c t t g g g t c a t c t c c t t t g t c c t t t g g g c t c c t g c c a t c t g g c c a c a c t a c t a g c c a g a c c g a a c c c a g t a g a g g a a a c a g g a a a c c c g a g g a c g g t a g a

1440 (in frame with 3xHA) LFWQYFVGKRTVPPGECFIQ LFWQYFVGKRTVPPGECFIQ t g t t c t g g c a a t a c t t t g t t g g a a a g a g a a c t g t g c c t c c g g g a g a g t g c t t c a t t c a g t a c a a g a c c g t t a t g a a a c a a c c t t t c t c t t g a c a c g g a g g c c c t c t c a c g a a g t a a g t c a

1500 (in frame with 3xHA) FLSEPTITFGTAIAAFYMPV FLSEPTITFGTAIAAFYMPV t c c t c a g t g a g c c c a c c a t t a c t t t t g g c a c a g c c a t c g c t g c t t t t t a t a t g c c t g t c a a g g a g t c a c t c g g g t g g t a a t g a a a a c c g t g t c g g t a g c g a c g a a a a a t a t a c g g a c a g t

1560 (in frame with 3xHA) TIMTILYWRIYKETEKRTKE TIMTILYWRIYKETEKRTKE c c a t t a t g a c t a t t t t a t a c t g g a g g a t c t a t a a g g a a a c t g a a a a g c g t a c c a a a g a g c g g t a a t a c t g a t a a a a t a t g a c c t c c t a g a t a t t c c t t t g a c t t t t c g c a t g g t t t c t c g

1620 (in frame with 3xHA) LAGLQASGTEAETENFVHPT LAGLQASGTEAETENFVHPT t t g c t g g c c t g c a a g c c t c t g g g a c a g a g g c a g a g a c a g a a a a c t t t g t c c a c c c c a c g g a a c g a c c g g a c g t t c g g a g a c c c t g t c t c c g t c t c t g t c t t t t g a a a c a g g t g g g g t g c c

1680 (in frame with 3xHA) GSSRSCSSYELQQQSMKRSN GSSRSCSSYELQQQSMKRSN g c a g t t c t c g a a g c t g c a g c a g t t a c g a a c t t c a a c a g c a a a g c a t g a a a c g c t c c a a c a c g t c a a g a g c t t c g a c g t c g t c a a t g c t t g a a g t t g t c g t t t c g t a c t t t g c g a g g t t g t

1740 (in frame with 3xHA) RRKYGRCHFWFTTKSWKPSS RRKYGRCHFWFTTKSWKPSS g g a g g a a g t a t g g c c g c t g c c a c t t c t g g t t c a c a a c c a a g a g c t g g a a a c c c a g c t c c g c c t c c t t c a t a c c g g c g a c g g t g a a g a c c a a g t g t t g g t t c t c g a c c t t t g g g t c g a g g c

1800 (in frame with 3xHA) EQMDQDHSSSDSWNNNDAAA EQMDQDHSSSDSWNNNDAAA a g c a g a t g g a c c a a g a c c a c a g c a g c a g t g a c a g t t g g a a c a a c a a t g a t g c t g c t g c c t t c g t c t a c c t g g t t c t g g t g t c g t c g t c a c t g t c a a c c t t g t t g t t a c t a c g a c g a c g g a

BsmBIEsp3I 1860 (in frame with 3xHA) SLENSASSDEEDIGSETRAI SLENSASSDEEDIGSETRAI c c c t g g a g a a c t c c g c c t c c t c c g a c g a g g a g g a c a t t g g c t c c g a g a c g a g a g c c a t c t g g g a c c t c t t g a g g c g g a g g a g g c t g c t c c t c c t g t a a c c g a g g c t c t g c t c t c g g t a g a

1920 (in frame with 3xHA) YSIVLKLPGHSTILNSTKLP YSIVLKLPGHSTILNSTKLP a c t c c a t c g t g c t c a a g c t t c c g g g t c a c a g c a c c a t c c t c a a c t c c a c c a a g t t a c c c t t g a g g t a g c a c g a g t t c g a a g g c c c a g t g t c g t g g t a g g a g t t g a g g t g g t t c a a t g g g a

SbfIPaqCI 1980 (in frame with 3xHA) SSDNLQVPEEELGMVDLERK SSDNLQVPEEELGMVDLERK c a t c g g a c a a c c t g c a g g t g c c t g a g g a g g a g c t g g g g a t g g t g g a c t t g g a g a g g a a a g g t a g c c t g t t g g a c g t c c a c g g a c t c c t c c t c g a c c c c t a c c a c c t g a a c c t c t c c t t t c

2040 (in frame with 3xHA) ADKLQAQKSVDDGGSFPKSF ADKLQAQKSVDDGGSFPKSF c c g a c a a g c t g c a g g c c c a g a a g a g c g t g g a c g a t g g a g g c a g t t t t c c a a a a a g c t t c t g g c t g t t c g a c g t c c g g g t c t t c t c g c a c c t g c t a c c t c c g t c a a a a g g t t t t t c g a a g a

2100 (in frame with 3xHA) SKLPIQLESAVDTAKTSDVN SKLPIQLESAVDTAKTSDVN c c a a g c t t c c c a t c c a g c t a g a g t c a g c c g t g g a c a c a g c t a a g a c t t c t g a c g t c a a c t g g t t c g a a g g g t a g g t c g a t c t c a g t c g g c a c c t g t g t c g a t t c t g a a g a c t g c a g t t g a

2160 (in frame with 3xHA) SSVGKSTATLPLSFKEATLA SSVGKSTATLPLSFKEATLA c c t c a g t g g g t a a g a g c a c g g c c a c t c t a c c t c t g t c c t t c a a g g a a g c c a c t c t g g c c a g g a g t c a c c c a t t c t c g t g c c g g t g a g a t g g a g a c a g g a a g t t c c t t c g g t g a g a c c g g t

2220 (in frame with 3xHA) KRFALKTRSQITKRKRMSLV KRFALKTRSQITKRKRMSLV a g a g g t t t g c t c t g a a g a c c a g a a g t c a g a t c a c t a a g c g g a a a a g g a t g t c c c t g g t c a t c t c c a a a c g a g a c t t c t g g t c t t c a g t c t a g t g a t t c g c c t t t t c c t a c a g g g a c c a g t

2280 (in frame with 3xHA) KEKKAAQTLSAILLAFIITW KEKKAAQTLSAILLAFIITW a g g a g a a g a a a g c g g c c c a g a c c c t c a g t g c g a t c t t g c t t g c c t t c a t c a t c a c t t g g a t c c t c t t c t t t c g c c g g g t c t g g g a g t c a c g c t a g a a c g a a c g g a a g t a g t a g t g a a c c t

2340 (in frame with 3xHA) TPYNIMVLVNTFCDSCIPKT TPYNIMVLVNTFCDSCIPKT c c c c a t a c a a c a t c a t g g t t c t g g t g a a c a c c t t t t g t g a c a g c t g c a t a c c c a a a a c c t g g g g t a t g t t g t a g t a c c a a g a c c a c t t g t g g a a a a c a c t g t c g a c g t a t g g g t t t t g g a

2400 (in frame with 3xHA) FWNLGYWLCYINSTVNPVCY FWNLGYWLCYINSTVNPVCY t t t g g a a t c t g g g c t a c t g g c t g t g c t a c a t c a a c a g c a c c g t g a a c c c c g t g t g c t a t g a a a c c t t a g a c c c g a t g a c c g a c a c g a t g t a g t t g t c g t g g c a c t t g g g g c a c a c g a t a c

2460 (in frame with 3xHA) ALCNKTFRTTFKMLLLCQCD ALCNKTFRTTFKMLLLCQCD c t c t g t g c a a c a a a a c a t t c a g a a c c a c t t t c a a g a t g c t g c t g c t g t g c c a g t g t g a c a g a g a c a c g t t g t t t t g t a a g t c t t g g t g a a a g t t c t a c g a c g a c g a c a c g g t c a c a c t g t

2520 (in frame with 3xHA) KKKRRKQQYQQRQSVIFHKR KKKRRKQQYQQRQSVIFHKR a a a a a a a g a g g c g c a a g c a g c a g t a c c a g c a g a g a c a g t c g g t c a t t t t t c a c a a g c g c g t t t t t t t c t c c g c g t t c g t c g t c a t g g t c g t c t c t g t c a g c c a g t a a a a a g t g t t c g c g c

AgeIPspOMIApaIBamHI 2580 (in frame with 3xHA) APEQALGARDPPVAT 1 5 MSKGA PS-CFP2 APEQALGARDPPVATMSKGA c a c c c g a g c a g g c c t t g g g g g c c c g g g a t c c a c c g g t c g c c a c c a t g a g c a a g g g c g c c g g t g g g c t c g t c c g g a a c c c c c g g g c c c t a g g t g g c c a g c g g t g g t a c t c g t t c c c g c g g c

2640 10 15 20 25 ELFTGIVPILIELNGDVNGH PS-CFP2 ELFTGIVPILIELNGDVNGH a g c t g t t c a c c g g c a t c g t g c c c a t c c t g a t c g a g c t g a a t g g c g a t g t g a a t g g c c a c a t c g a c a a g t g g c c g t a g c a c g g g t a g g a c t a g c t c g a c t t a c c g c t a c a c t t a c c g g t g t

2700 30 35 40 45 KFSVSGEGEGDATYGKLTLK PS-CFP2 KFSVSGEGEGDATYGKLTLK a g t t c a g c g t g a g c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c t c g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a

Bpu10IBstXI 2760 50 55 60 65 FICTTGKLPVPWPTLVATLS PS-CFP2 FICTTGKLPVPWPTLVATLS t c a t c t g c a c c a c c g g c a a g c t g c c t g t g c c c t g g c c c a c c c t g g t g g c c a c c c t g a g c t a g t a g a c g t g g t g g c c g t t c g a c g g a c a c g g g a c c g g g t g g g a c c a c c g g t g g g a c t c g a

2820 70 75 80 85 YGVQCFSRYPDHMKQHDFFK PS-CFP2 YGVQCFSRYPDHMKQHDFFK a c g g c g t g c a g t g c t t c t c a c g c t a c c c c g a t c a c a t g a a g c a g c a c g a c t t c t t c a a g a t g c c g c a c g t c a c g a a g a g t g c g a t g g g g c t a g t g t a c t t c g t c g t g c t g a a g a a g t t c t

2880 90 95 100 105 SAMPEGYIQERTIFFEDDGN PS-CFP2 SAMPEGYIQERTIFFEDDGN g c g c c a t g c c t g a g g g c t a c a t c c a g g a g c g c a c c a t c t t c t t c g a g g a t g a c g g c a a c t c g c g g t a c g g a c t c c c g a t g t a g g t c c t c g c g t g g t a g a a g a a g c t c c t a c t g c c g t t g a

2940 110 115 120 125 YKTRAEVKFEGDTLVSRIEL PS-CFP2 YKTRAEVKFEGDTLVSRIEL a c a a g a c g c g c g c c g a g g t g a a g t t c g a g g g c g a t a c c c t g g t g a g t c g c a t c g a g c t g a t g t t c t g c g c g c g g c t c c a c t t c a a g c t c c c g c t a t g g g a c c a c t c a g c g t a g c t c g a c t

3000 130 135 140 145 TGTDFKEDGNILGNKMEYNY PS-CFP2 TGTDFKEDGNILGNKMEYNY c c g g c a c t g a t t t c a a g g a g g a t g g c a a c a t c c t g g g c a a t a a g a t g g a g t a c a a c t a c a g g c c g t g a c t a a a g t t c c t c c t a c c g t t g t a g g a c c c g t t a t t c t a c c t c a t g t t g a t g t

3060 150 155 160 165 NATNVYIVADKARNGIKVNF PS-CFP2 NATNVYIVADKARNGIKVNF a c g c c a c c a a t g t g t a c a t c g t g g c c g a c a a g g c c a g g a a t g g c a t c a a g g t g a a c t t c a t g c g g t g g t t a c a c a t g t a g c a c c g g c t g t t c c g g t c c t t a c c g t a g t t c c a c t t g a a g t

3120 170 175 180 185 KVRHNIKDGSVQLADHYQQN PS-CFP2 KVRHNIKDGSVQLADHYQQN a g g t c c g c c a c a a c a t c a a g g a t g g c a g c g t g c a g c t g g c c g a c c a c t a c c a g c a g a a t a t c c a g g c g g t g t t g t a g t t c c t a c c g t c g c a c g t c g a c c g g c t g g t g a t g g t c g t c t t a t

3180 190 195 200 205 TPIGDGPVLLPDNHYLSTQS PS-CFP2 TPIGDGPVLLPDNHYLSTQS c c c c c a t c g g c g a t g g c c c t g t g c t g c t g c c c g a t a a c c a c t a c c t g t c c a c c c a g a g c g g g g g g t a g c c g c t a c c g g g a c a c g a c g a c g g g c t a t t g g t g a t g g a c a g g t g g g t c t c g c

PspXIPpuMI 3240 210 215 220 225 ALSKDPNEKRDHMIYLEFVT PS-CFP2 ALSKDPNEKRDHMIYLEFVT c c c t g t c c a a g g a c c c c a a c g a g a a g c g c g a t c a c a t g a t c t a c c t c g a g t t c g t g a c c g g g g a c a g g t t c c t g g g g t t g c t c t t c g c g c t a g t g t a c t a g a t g g a g c t c a a g c a c t g g c

XbaI*NotI 3300 230 235 AAAITHGMDELYK* PS-CFP2 AAAITHGMDELYK* c c g c c g c c a t c a c c c a c g g c a t g g a t g a g c t g t a c a a g t a a a g c g g c c g c g a c t c t a g a t g g c g g c g g t a g t g g g t g c c g t a c c t a c t c g a c a t g t t c a t t t c g c c g g c g c t g a g a t c t a

3360 c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a

HpaIMfeI 3420 SV40 poly(A) signal c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g

3480 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g

3540 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t

3600 f1 ori g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g

3660 f1 ori a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t

3720 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c

DraIII 3780 f1 ori g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a

3840 f1 ori t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t

3900 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c

3960 f1 ori c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c

4020 f1 ori AmpR promoter c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t

4080 AmpR promoter c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g

4140 AmpR promoter SV40 promoter SV40 enhancer c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a

4200 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a

SexAI*CsiI 4260 SV40 promoter SV40 enhancer g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g

4320 SV40 promoter SV40 enhancer a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g

4380 SV40 promoter SV40 ori t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a

4440 SV40 promoter SV40 ori a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t

4500 SV40 promoter SV40 ori g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c

4560 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR MIEQDGLHAGSPAAWV g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c

4620 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R ERLFGYDWAQQTIGCSDAAV a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a

4680 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR FRLSAQGRPVLFVKTDLSGA t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g

4740 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR LNELQDEAARLSWLATTGVP t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a

Tth111IPflFIFspI 4800 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR CAAVLDVVTEAGRDWLLLGE g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c

4860 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR VPGQDLLSSHLAPAEKVSIM *RVKSRGLFYGDH t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c

4920 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR ADAMRRLHTLDPATCPFDHQ SICHPPQMRKIRSGAWEVVL c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c

4980 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR AKHRIERARTRMEAGLVDQD RFMADLSCTSPHFGTKDILI c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c

5040 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR DLDEEHQGLAPAELFARLKA IQVFLMLPERWGFQEGPELR a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t

5100 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR SMPDGEDLVVTHGDACLPNI AHGVALIEDHGMAIGAQRID g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t

RsrII 5160 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR MVENGRFSGFIDCGRLGVAD HHFIAAKRSEDVTAPQTHRV t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g

5220 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F RYQDIALATRDIAEELGGEW AILVYRQSGTINSFLKAAFP g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c

5280 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR ADRFLVLYGIAAPDSQRIAF SVAEEHKVTDGSGIRLADGE c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a

5340 260 265 YRLLDEFF* NeoR/KanR 260 265 YRLLDEFF* NeoR/KanR MTDQA YRLLDEFF* IAKKVLEESRSEPTRFSRGL a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g

5400 TPNLPSRDFDSTAAFYERLG SAWGAM g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c

5460 FGIVFRDAGWMILQRGDLML c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a

5520 A C T T T G T G C C T T C C T C T G T T TK-pA-R EFFAHPRGRLTETRKETIPE g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t

5580 HSV TK poly(A) signal GTRAMTAIKRQNKTHGVGSF a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a

BsaI 5640 HSV TK poly(A) signal VHKRGVRSQGWHSVDTPPRP t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g

5700 HWGQYARVSSFSPPHPPSSG c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c

5760 EGPGLAANVGAAGPAIASGY t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t

5820 SYIL* c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t

5880 g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g

5940 ori g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a

6000 ori c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t

6060 ori g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a

6120 ori t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t

6180 ori c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g

6240 ori c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c

ApaLI 6300 ori t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c

6360 ori t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c

6420 ori G G G A A A C G C C T G G T A T C T pBR322ori-F c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a

6480 ori T T pBR322ori-F t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g

6540 ori a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a

AflIIIPciI 3ʹ 5ʹ 6579 t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.