Addgene pDEST/LIfeAct-mCherry-N1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pDEST/LIfeAct-mCherry-N1 Sequencing Result


Map View

AflIII - PciI (4766) pBR322ori-F (4611 .. 4630) EcoO109I (3946) BsaI (3837) TK-pA-R (3702 .. 3721) PfoI (3623) Neo-F (3384 .. 3403) RsrII (3364) BsrDI (3081) PflFI - Tth111I (2966) FspI (2950) Neo-R (2774 .. 2793) BspDI * - ClaI * (2688) SfiI (2623) CsiI - SexAI * (2437) AseI (0) NdeI (227) SnaBI (333) CMV-F (512 .. 532) NheI (584) BmtI (588) XmnI (630) SalI (637) AccI (638) BclI * (665) EcoRV (706) attB2 BamHI (744) mCherry-R (902 .. 920) AhdI (951) PstI - SbfI (1112) mCherry-F (1170 .. 1189) BbsI (1192) PflMI (1196) BbvCI - Bpu10I (1295) BsgI (1351) SgrAI (1438) XcmI (1443) EcoRI (1468) NotI (1492) MfeI (1598) HpaI (1611) SV40pA-R (1649 .. 1668) BtsI - BtsαI (1687) EBV-rev (1703 .. 1722) AflII (1730) F1ori-F (1877 .. 1898) DraIII (1964) F1ori-R (2089 .. 2108) pBABE 3' (2351 .. 2371) pDEST/LIfeAct-mCherry-N1 4831 bp

Sequence View

AseI 5ʹ 3ʹ 60 CMV enhancer t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t

120 CMV enhancer t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t

180 CMV enhancer a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c

NdeI 240 CMV enhancer g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c

300 CMV enhancer c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g

SnaBI 360 CMV enhancer CMV promoter t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c

420 CMV promoter a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t

480 CMV promoter a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c

BmtINheI 600 CMV promoter attB1 g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c a t c a a a c a a g t c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g t a g t t t g t t c a

SalIAccIXmnI 660 1 MGVA attB1 Lifeact MGVA t t g t a c a a a a a a g c a g g c t t t a a a g g a a c c a a t t c a g t c g a c t g g a t c a t g g g c g t g g c c a a c a t g t t t t t t c g t c c g a a a t t t c c t t g g t t a a g t c a g c t g a c c t a g t a c c c g c a c c g g

EcoRVBclI* 720 5 10 15 DLIKKFESISKEE (in frame with Lifeact) SRYLDPA Lifeact attB2 DLIKKFESISKEESRYLDPA *VWS g a c c t g a t c a a g a a g t t c g a g a g c a t c a g c a a g g a g g a g t c g a g a t a t c t a g a c c c a g c t c t g g a c t a g t t c t t c a a g c t c t c g t a g t c g t t c c t c c t c a g c t c t a t a g a t c t g g g t c g a

BamHI 780 (in frame with Lifeact) FLYKVVRWDPAT 1 5 MVSKGEED mCherry attB2 Kozak sequence FLYKVVRWDPATMVSKGEED EQVFHNSPIGGGHHALALLI t t c t t g t a c a a a g t g g t t c g a t g g g a t c c c g c c a c c a t g g t g a g c a a g g g c g a g g a g g a t a a g a a c a t g t t t c a c c a a g c t a c c c t a g g g c g g t g g t a c c a c t c g t t c c c g c t c c t c c t a

840 10 15 20 25 NMAIIKEFMRFKVHMEGSVN mCherry NMAIIKEFMRFKVHMEGSVN VHGDDLLEHAELHVHLAGHV a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g

900 30 35 40 45 GHEFEIEGEGEGRPYEGTQT mCherry GHEFEIEGEGEGRPYEGTQT AVLELDLALALAAGVLAGLG g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g

AhdI 960 50 55 60 65 AKLKVTKGGPLPFAWDILSP mCherry G G T T C G A C T T C C A C T G G T T mCherry-R AKLKVTKGGPLPFAWDILSP GLQLHGLTAGQGEGPVDQGR g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g a

1020 70 75 80 85 QFMYGSKAYVKHPADIPDYL mCherry QFMYGSKAYVKHPADIPDYL LEHVAGLGVHLVGGVDGVVQ c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g c a c c c c g c c g a c a t c c c c g a c t a c t t g g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t c g t g g g g c g g c t g t a g g g g c t g a t g a a c

1080 90 95 100 105 KLSFPEGFKWERVMNFEDGG mCherry KLSFPEGFKWERVMNFEDGG LQGEGLAELPLAHHVELVAA a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g

SbfIPstI 1140 110 115 120 125 VVTVTQDSSLQDGEFIYKVK mCherry VVTVTQDSSLQDGEFIYKVK HHGHGLVGGQLVALEDVLHL g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c t g c c g c t c a a g t a g a t g t t c c a c t t c

PflMIBbsI 1200 130 135 140 145 LRGTNFPSDGPVMQKKTMGW mCherry C C C C G T A A T G C A G A A G A A G A mCherry-F LRGTNFPSDGPVMQKKTMGW QAAGVEGGVAGYHLLLGHAP c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c

1260 150 155 160 165 EASSERMYPEDGALKGEIKQ mCherry EASSERMYPEDGALKGEIKQ LGGGLPHVGLVAGQLALDLL g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a c g g c g c c c t g a a g g g c g a g a t c a a g c a g c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t g c c g c g g g a c t t c c c g c t c t a g t t c g t c

Bpu10IBbvCI 1320 170 175 180 185 RLKLKDGGHYDAEVKTTYKA mCherry RLKLKDGGHYDAEVKTTYKA PQLQLVAAVVVSLDLGGVLG a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a c g c t g a g g t c a a g a c c a c c t a c a a g g c c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t g c g a c t c c a g t t c t g g t g g a t g t t c c g g

BsgI 1380 190 195 200 205 KKPVQLPGAYNVNIKLDITS mCherry KKPVQLPGAYNVNIKLDITS LLGHLQGAGVVDVDLQVDGG a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a c g t c a a c a t c a a g t t g g a c a t c a c c t c c t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g

SgrAI 1440 210 215 220 225 HNEDYTIVEQYERAEGRHST mCherry HNEDYTIVEQYERAEGRHST VVLVVGDHFLVFAGLAAVGG c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g

NotIEcoRIXcmI 1500 230 235 GGMDELYK* mCherry GGMDELYK* AAHVLQVLLFEFSYLYLPRS g g c g g c a t g g a c g a g c t g t a c a a g t a a g a a t t c g a a g c t g t a c a a g t a a a g c g g c c g c g a c c g c c g t a c c t g c t c g a c a t g t t c a t t c t t a a g c t t c g a c a t g t t c a t t t c g c c g g c g c t

1560 ELDYDAM c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g

HpaIMfeI 1620 SV40 poly(A) signal c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a

1680 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t

AflIIBtsαIBtsI 1740 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t

1800 f1 ori a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a

1860 f1 ori t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a

1920 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t

DraIII 1980 f1 ori c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t

2040 f1 ori a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g

2100 f1 ori A G G G A A G A A A G C F1ori-R c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g

2160 f1 ori G A A A G G A G F1ori-R g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g

2220 f1 ori a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a

2280 AmpR promoter g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c

2340 AmpR promoter SV40 promoter SV40 enhancer a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c

2400 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g

SexAI*CsiI 2460 SV40 promoter SV40 enhancer a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g

2520 SV40 promoter SV40 enhancer t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c

2580 SV40 promoter SV40 ori c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a

SfiI 2640 SV40 promoter SV40 ori g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g

BspDI*ClaI* 2700 SV40 promoter SV40 ori c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g

2760 1 5 10 MIEQDGLHAGSPAA NeoR/KanR 1 5 10 MIEQDGLHAGSPAA NeoR/KanR MIEQDGLHAGSPAA a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g

2820 15 20 25 30 WVERLFGYDWAQQTIGCSDA NeoR/KanR 15 20 25 30 WVERLFGYDWAQQTIGCSDA NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R WVERLFGYDWAQQTIGCSDA t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g

2880 35 40 45 50 AVFRLSAQGRPVLFVKTDLS NeoR/KanR 35 40 45 50 AVFRLSAQGRPVLFVKTDLS NeoR/KanR AVFRLSAQGRPVLFVKTDLS c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g

2940 55 60 65 70 GALNELQDEAARLSWLATTG NeoR/KanR 55 60 65 70 GALNELQDEAARLSWLATTG NeoR/KanR GALNELQDEAARLSWLATTG c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c

Tth111IPflFIFspI 3000 75 80 85 90 VPCAAVLDVVTEAGRDWLLL NeoR/KanR 75 80 85 90 VPCAAVLDVVTEAGRDWLLL NeoR/KanR VPCAAVLDVVTEAGRDWLLL c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a

3060 95 100 105 110 GEVPGQDLLSSHLAPAEKVS NeoR/KanR 95 100 105 110 GEVPGQDLLSSHLAPAEKVS NeoR/KanR GEVPGQDLLSSHLAPAEKVS *RVKSRGLFYG g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g

BsrDI 3120 115 120 125 130 IMADAMRRLHTLDPATCPFD NeoR/KanR 115 120 125 130 IMADAMRRLHTLDPATCPFD NeoR/KanR IMADAMRRLHTLDPATCPFD DHSICHPPQMRKIRSGAWEV c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t

3180 135 140 145 150 HQAKHRIERARTRMEAGLVD NeoR/KanR 135 140 145 150 HQAKHRIERARTRMEAGLVD NeoR/KanR HQAKHRIERARTRMEAGLVD VLRFMADLSCTSPHFGTKDI c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t

3240 155 160 165 170 QDDLDEEHQGLAPAELFARL NeoR/KanR 155 160 165 170 QDDLDEEHQGLAPAELFARL NeoR/KanR QDDLDEEHQGLAPAELFARL LIIQVFLMLPERWGFQEGPE t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a

3300 175 180 185 190 KASMPDGEDLVVTHGDACLP NeoR/KanR 175 180 185 190 KASMPDGEDLVVTHGDACLP NeoR/KanR KASMPDGEDLVVTHGDACLP LRAHGVALIEDHGMAIGAQR c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g

3360 195 200 205 210 NIMVENGRFSGFIDCGRLGV NeoR/KanR 195 200 205 210 NIMVENGRFSGFIDCGRLGV NeoR/KanR NIMVENGRFSGFIDCGRLGV IDHHFIAAKRSEDVTAPQTH g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a

RsrII 3420 215 220 225 230 ADRYQDIALATRDIAEELGG NeoR/KanR 215 220 225 230 ADRYQDIALATRDIAEELGG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F ADRYQDIALATRDIAEELGG RVAILVYRQSGTINSFLKAA g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c

3480 235 240 245 250 EWADRFLVLYGIAAPDSQRI NeoR/KanR 235 240 245 250 EWADRFLVLYGIAAPDSQRI NeoR/KanR EWADRFLVLYGIAAPDSQRI FPSVAEEHKVTDGSGIRLAD c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a

3540 255 260 265 AFYRLLDEFF* NeoR/KanR 255 260 265 AFYRLLDEFF* NeoR/KanR MT AFYRLLDEFF* GEIAKKVLEESRSEPTRFSR c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g

3600 DQATPNLPSRDFDSTAAFYE GLSAWGAM g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t

PfoI 3660 RLGFGIVFRDAGWMILQRGD a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a

3720 A C T T T G T G C C T T C C T C T G T TK-pA-R LMLEFFAHPRGRLTETRKET c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t

3780 HSV TK poly(A) signal T TK-pA-R IPEGTRAMTAIKRQNKTHGV a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a

BsaI 3840 HSV TK poly(A) signal GSFVHKRGVRSQGWHSVDTP g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t

3900 PRPHWGQYARVSSFSPPHPP c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t

EcoO109I 3960 SSGEGPGLAANVGAAGPAIA a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g

4020 SGYSYIL* t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g

4080 t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g

4140 ori c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c

4200 ori c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c

4260 ori g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t

4320 ori a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c

4380 ori c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c

4440 ori t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t

4500 ori a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g

4560 ori c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a

4620 ori G G G A A A C G C C pBR322ori-F c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g

4680 ori T G G T A T C T T T pBR322ori-F t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t

4740 ori t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g

AflIIIPciI 4800 c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c

3ʹ 5ʹ 4831 g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.