Addgene pOPH6 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pOPH6 Sequencing Result


Map View

F1ori-R (5677 .. 5696) NaeI (5655) NgoMIV (5653) F1ori-F (5465 .. 5486) Amp-R (4960 .. 4979) BsaI (4471) pBR322ori-F (3658 .. 3677) PciI (3517) L4440 (3407 .. 3424) BspQI - SapI (3401) CAP binding site M13/pUC Reverse (3189 .. 3211) lac operator M13 Reverse (3176 .. 3192) M13 rev EcoO109I (3114) pBluescriptKS (3093 .. 3109) AbsI - PaeR7I - PspXI - XhoI (3105) KS primer SalI (3099) BspDI - ClaI (3091) M13/pUC Forward (104 .. 126) M13 Forward (118 .. 135) T7 (142 .. 161) T7 promoter Eco53kI (171) SacI (173) AleI (179) SacII (180) NotI (186) XbaI (193) NruI (232) EcoRI (278) MscI (649) BstZ17I (687) NdeI (873) BstBI (940) PfoI (1005) TspMI - XmaI (1041) SmaI (1043) BamHI (1230) BsaBI * (1235) BmgBI (1277) AgeI (1732) EcoNI (1991) BfuAI - BspMI (2095) EcoRV (2201) BssHII (2236) BstEII (2465) LacI-R (2964 .. 2983) pOPH6 5806 bp

Sequence View

5ʹ 3ʹ 60 QQSPRDTRAEESNRWSAFP a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g g g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c

120 M13 fwd C C C A G T C A C G A C G T T G T M13/pUC Forward T G T M13 Forward PHAALRNLQTVGPNEWDRRQ g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t t g t c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a a c a

Eco53kISacIAleISacII 180 M13 fwd T7 promoter A A A A C G M13/pUC Forward A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C A C T A T A G G G T7 LVVALSNYYSESYPSNSSWR a a a a c g a c g g c c a g t g a a t t g t a a t a c g a c t c a c t a t a g g g c g a a t t g g a g c t c c a c c g c t t t t g c t g c c g g t c a c t t a a c a t t a t g c t g a g t g a t a t c c c g c t t a a c c t c g a g g t g g c g

NruIXbaINotI 240 1 5 MKKTAIAIA OmpA signal peptide MKKTAIAIA PPPRELYRPCFIFFVAIAIA g g t g g c g g c c g c t c t a g a t a a c g a g g g c a a a a a a t g a a a a a g a c a g c t a t c g c g a t t g c a c c a c c g c c g g c g a g a t c t a t t g c t c c c g t t t t t t a c t t t t t c t g t c g a t a g c g c t a a c g t

EcoRI 300 10 15 20 VALAGFATVAQA (in frame with OmpA signal peptide) GIPAPADN OmpA signal peptide VALAGFATVAQAGIPAPADN TASAPKAVTACAPIGAGASL g t g g c a c t g g c t g g t t t c g c t a c c g t a g c g c a g g c c g g a a t t c c a g c t c c g g c a g a t a a t c a c c g t g a c c g a c c a a a g c g a t g g c a t c g c g t c c g g c c t t a a g g t c g a g g c c g t c t a t t a

360 (in frame with OmpA signal peptide) TVNIKTFDKVKNAFGDGLSQ TVNIKTFDKVKNAFGDGLSQ VTFILVNSLTFFAKPSPNDW a c c g t a a a t a t t a a a a c a t t c g a c a a a g t a a a a a a t g c c t t t g g t g a c g g a t t g t c c c a a t g g c a t t t a t a a t t t t g t a a g c t g t t t c a t t t t t t a c g g a a a c c a c t g c c t a a c a g g g t t

420 (in frame with OmpA signal peptide) SAEGTFTFPADVTAVKTIKM SAEGTFTFPADVTAVKTIKM LASPVKVNGASTVATFVILI a g t g c g g a a g g a a c c t t t a c a t t t c c g g c c g a t g t a a c a g c c g t a a a a a c g a t t a a g a t g t c a c g c c t t c c t t g g a a a t g t a a a g g c c g g c t a c a t t g t c g g c a t t t t t g c t a a t t c t a c

480 (in frame with OmpA signal peptide) FIKNECPNKTCDEWDRYANV FIKNECPNKTCDEWDRYANV NM t t c a t t a a a a a t g a a t g t c c t a a t a a a a c t t g t g a t g a a t g g g a t c g t t a t g c c a a t g t t a a g t a a t t t t t a c t t a c a g g a t t a t t t t g a a c a c t a c t t a c c c t a g c a a t a c g g t t a c a a

540 (in frame with OmpA signal peptide) YVKNKTTGEWYEIGRFITPY YVKNKTTGEWYEIGRFITPY t a t g t a a a a a a t a a a a c a a c a g g t g a g t g g t a c g a a a t a g g a c g c t t t a t t a c t c c a t a t a t a c a t t t t t t a t t t t g t t g t c c a c t c a c c a t g c t t t a t c c t g c g a a a t a a t g a g g t a t a

600 (in frame with OmpA signal peptide) WVGTEKLPRGLEIDVTDFKS WVGTEKLPRGLEIDVTDFKS t g g g t g g g a a c g g a a a a a t t a c c t c g t g g a c t g g a a a t t g a t g t t a c a g a t t t c a a a t c t a c c c a c c c t t g c c t t t t t a a t g g a g c a c c t g a c c t t t a a c t a c a a t g t c t a a a g t t t a g a

MscI 660 (in frame with OmpA signal peptide) LLSGNTELKIYTETWLAKGR LLSGNTELKIYTETWLAKGR t t a c t a t c c g g a a a t a c a g a a c t t a a a a t t t a t a c g g a g a c a t g g c t g g c c a a a g g a a g a a a t g a t a g g c c t t t a t g t c t t g a a t t t t a a a t a t g c c t c t g t a c c g a c c g g t t t c c t t c t

BstZ17I 720 (in frame with OmpA signal peptide) EYSVDFDIVYGTPDYKYSAV EYSVDFDIVYGTPDYKYSAV g a a t a c a g t g t a g a t t t c g a t a t t g t a t a c g g g a c a c c g g a t t a t a a a t a t t c g g c t g t a c t t a t g t c a c a t c t a a a g c t a t a a c a t a t g c c c t g t g g c c t a a t a t t t a t a a g c c g a c a t

780 (in frame with OmpA signal peptide) VPVVQYNKSSIDGVPYGKAH VPVVQYNKSSIDGVPYGKAH g t a c c t g t a g t t c a g t a t a a c a a a t c a t c t a t t g a c g g a g t c c c t t a t g g t a a a g c a c a t c a t g g a c a t c a a g t c a t a t t g t t t a g t a g a t a a c t g c c t c a g g g a a t a c c a t t t c g t g t a

840 (in frame with OmpA signal peptide) TLALKKNIQLPTNTEKAYLR TLALKKNIQLPTNTEKAYLR a c a t t g g c t t t g a a a a a g a a t a t c c a g t t a c c a a c a a a c a c a g a a a a a g c t t a t c t t a g a t g t a a c c g a a a c t t t t t c t t a t a g g t c a a t g g t t g t t t g t g t c t t t t t c g a a t a g a a t c t

NdeI 900 (in frame with OmpA signal peptide) TTISGWGHAKPYDAGSRGCA TTISGWGHAKPYDAGSRGCA a c t a c t a t t t c c g g a t g g g g a c a t g c t a a g c c a t a t g a t g c g g g a a g c a g a g g t t g t g c a t g a t g a t a a a g g c c t a c c c c t g t a c g a t t c g g t a t a c t a c g c c c t t c g t c t c c a a c a c g t

BstBI 960 (in frame with OmpA signal peptide) EWCFRTHTIAINNSNTFQHQ EWCFRTHTIAINNSNTFQHQ g a a t g g t g c t t c a g a a c a c a c a c t a t a g c a a t a a a t a a t t c g a a t a c t t t c c a g c a t c a g c t t a c c a c g a a g t c t t g t g t g t g a t a t c g t t a t t t a t t a a g c t t a t g a a a g g t c g t a g t c

PfoI 1020 (in frame with OmpA signal peptide) LGALGCSANPINNQSPGNWT LGALGCSANPINNQSPGNWT c t g g g t g c t t t a g g a t g t t c a g c a a a c c c t a t c a a t a a t c a g a g t c c g g g a a a t t g g a c t g a c c c a c g a a a t c c t a c a a g t c g t t t g g g a t a g t t a t t a g t c t c a g g c c c t t t a a c c t g a

TspMIXmaISmaI 1080 (in frame with OmpA signal peptide) PDRAGWCPGMAVPTRIDVLN PDRAGWCPGMAVPTRIDVLN c c c g a c a g a g c c g g t t g g t g c c c g g g a a t g g c a g t t c c a a c a c g t a t a g a t g t a c t g a a t g g g c t g t c t c g g c c a a c c a c g g g c c c t t a c c g t c a a g g t t g t g c a t a t c t a c a t g a c t t a

1140 (in frame with OmpA signal peptide) NSLIGSTFSYEYKFQNWTNN NSLIGSTFSYEYKFQNWTNN a a t t c t t t a a t a g g c a g t a c t t t t a g t t a t g a a t a t a a a t t c c a g a a c t g g a c a a a t a a c t t a a g a a a t t a t c c g t c a t g a a a a t c a a t a c t t a t a t t t a a g g t c t t g a c c t g t t t a t t g

1200 (in frame with OmpA signal peptide) GTNGDAFYAISSFVIAKSNT GTNGDAFYAISSFVIAKSNT g g a a c c a a t g g a g a t g c t t t t t a t g c a a t t t c c a g t t t t g t g a t t g c a a a a a g t a a t a c a c c t t g g t t a c c t c t a c g a a a a a t a c g t t a a a g g t c a a a a c a c t a a c g t t t t t c a t t a t g t

BamHIBsaBI* 1260 (in frame with OmpA signal peptide) PISAPVVTNLDP 1 5 HHHHHH * 6xHis PISAPVVTNLDPHHHHHH* c c t a t t a g t g c t c c g g t a g t t a c a a a c t t g g a t c c g c a t c a c c a t c a c c a c c a t t g a c g a g g a t a a t c a c g a g g c c a t c a a t g t t t g a a c c t a g g c g t a g t g g t a g t g g t g g t a a c t g c t

BmgBI 1320 t c c g g c t g a g c a a c g a c g t g a a c g c a a t g c g t t c c g a c g t t c a g g c t g c t a a a g a t g a c g a g g c c g a c t c g t t g c t g c a c t t g c g t t a c g c a a g g c t g c a a g t c c g a c g a t t t c t a c t g c

1380 c a g c t c g t g c t a a c c a g c g t c t g g a c a a c a t g g c t a c t a a a t a c c g c a a g t a a t a g t a c c g t c g a g c a c g a t t g g t c g c a g a c c t g t t g t a c c g a t g a t t t a t g g c g t t c a t t a t c a t g g

1440 * t g t g a a g t g a a a a a t g g c g c a c a t t g t g c g a c a t t t t t t t t g t c t g c c g t t t a c c g c t a c a c a c t t c a c t t t t t a c c g c g t g t a a c a c g c t g t a a a a a a a a c a g a c g g c a a a t g g c g a t g

1500 QTVRLMNGQEPEGNQASEVS t g c g t c a c g c g t a a c a t a t t c c c t t g c t c t g g t t c a c c a t t c t g c g c t g a c t c t a c t g a a a c g c a g t g c g c a t t g t a t a a g g g a a c g a g a c c a a g t g g t a a g a c g c g a c t g a g a t g a c t t

1560 PANSAAPTAGSSVSVPYGAR g g c g c a t t g c t g g c t g c g g g a g t t g c t c c a c t g c t c a c c g a a a c c g g a t a c c c t g c c c g a c c g c g t a a c g a c c g a c g c c c t c a a c g a g g t g a c g a g t g g c t t t g g c c t a t g g g a c g g g c t

1620 RYLAKDVLKQDVAKNDKFQT c g a t a c a a c g c t t t a t c g a c t a a c t t c t g a t c t a c a g c c t t a t t g t c t t t a a a t t g c g t a g c t a t g t t g c g a a a t a g c t g a t t g a a g a c t a g a t g t c g g a a t a a c a g a a a t t t a a c g c a t

1680 FGAPLTYPMTQVNQQEEASL a a g c c t g c t g g c a g t g t g t a t g g c a t t g t c t g a a c g t t c t g c t g t t c t t c t g c c g a t a g t t t c g g a c g a c c g t c a c a c a t a c c g t a a c a g a c t t g c a a g a c g a c a a g a a g a c g g c t a t c a

AgeI 1740 PRHVEVYRMGNPEVSYKVPE g g t c g a t g t a c t t c a a c a t a a c g c a t c c c g t t a g g c t c c a c g g a a t a t t t c a c c g g t t c g c c a g c t a c a t g a a g t t g t a t t g c g t a g g g c a a t c c g a g g t g c c t t a t a a a g t g g c c a a g c

1800 NIVKVPTGTRVSSFLAKIDP t t g a t c a c t t t c a c c g g c g t t c c c g t c c g c a c g c t g g a g a a c a a g g c t t t a a t a t c c g g t a a c t a g t g a a a g t g g c c g c a a g g g c a g g c g t g c g a c c t c t t g t t c c g a a a t t a t a g g c c a

1860 ANMRICGSSVRLGVSDPAST g c a t t c a t g c g a a t a c a c c c t g a a c t g a c g c g c a a a c c g a c g c t g t c c g g c g c a c t g g t a c g t a a g t a c g c t t a t g t g g g a c t t g a c t g c g c g t t t g g c t g c g a c a g g c c g c g t g a c c a t

1920 GHILYEGNGHALRLAYRGLP c c a t g a a t g a g g t a t t c g c c a t t a c c a t g c g c g a g g c g c a g t g c g t a a c g t c c t a g c g g g g g t a c t t a c t c c a t a a g c g g t a a t g g t a c g c g c t c c g c g t c a c g c a t t g c a g g a t c g c c c

1980 NNPGAPVVPPLKIGRELSRQ t t a t t t g g t c c g g c a g g a a c g a c t g g c g g t a a t t t a a t g c c a c g c t c c a g c g a a c g c t g a a a t a a a c c a g g c c g t c c t t g c t g a c c g c c a t t a a a t t a c g g t g c g a g g t c g c t t g c g a c t

EcoNI 2040 RIGATPTWTPNPIKQGVRTE c g a a t g c c t g c c g t a g g c g t c c a g g t t g g g t t a g g g a t t t t c t g c c c a a c a c g c g t t t c c g c t t a c g g a c g g c a t c c g c a g g t c c a a c c c a a t c c c t a a a a g a c g g g t t g t g c g c a a a g g

BspMIBfuAI 2100 MVPTELGQLGIGIPYVQVIN a t c a c c g g c g t t t c c a g c c c c t g c a a t c c a a t a c c t a t t g g a t a a a c c t g c a c a a t a t t t t a g t g g c c g c a a a g g t c g g g g a c g t t a g g t t a t g g a t a a c c t a t t t g g a c g t g t t a t a a a

2160 EGPPYYYLRLEALNTLPPIY t c t c c c g g c g g a t a a t a a t a a a g g c g c a g c t c t g c a a g g t t g g t c a a c g g c g g g a t a t a a a g a g g g c c g c c t a t t a t t a t t t c c g c g t c g a g a c g t t c c a a c c a g t t g c c g c c c t a t a t t

EcoRV 2220 CSSDETDDYGVVSIDAGVRL c a t g a g c t g t c t t c g g t a t c g t c g t a t c c c a c t a c c g a g a t a t c c g c a c c a a c g c g c a g c g t a c t c g a c a g a a g c c a t a g c a g c a t a g g g t g a t g g c t c t a t a g g c g t g g t t g c g c g t c g

BssHII 2280 GSETIARMAGLAMQDNAVLM c c g g a c t c g g t a a t g g c g c g c a t t g c g c c c a g c g c c a t c t g a t c g t t g g c a a c c a g c a t c g g c c t g a g c c a t t a c c g c g c g t a a c g c g g g t c g c g g t a g a c t a g c a a c c g t t g g t c g t a g

2340 ATPVIGENLMQMTQQFGSMA g c a g t g g g a a c g a t g c c c t c a t t c a g c a t t t g c a t g g t t t g t t g a a a a c c g g a c a t g g c a c g t c a c c c t t g c t a c g g g a g t a a g t c g t a a a c g t a c c a a a c a a c t t t t g g c c t g t a c c g t

2400 SWDGEREAIPQIQNRTLYKH c t c c a g t c g c c t t c c c g t t c c g c t a t c g g c t g a a t t t g a t t g c g a g t g a g a t a t t t a t g c g a g g t c a g c g g a a g g g c a a g g c g a t a g c c g a c t t a a a c t a a c g c t c a c t c t a t a a a t a c g

2460 WGALRLRASVSSLPGALLAI c a g c c a g c c a g a c g c a g a c g c g c c g a g a c a g a a c t t a a t g g g c c c g c t a a c a g c g c g a t t g t c g g t c g g t c t g c g t c t g c g c g g c t c t g t c t t g a a t t a c c c g g g c g a t t g t c g c g c t a a

BstEII 2520 QQHGLAVLHEVGLRTGDEHS t g c t g g t g a c c c a a t g c g a c c a g a t g c t c c a c g c c c a g t c g c g t a c c g t c t t c a t g g g a g a c g a c c a c t g g g t t a c g c t g g t c t a c g a g g t g c g g g t c a g c g c a t g g c a g a a g t a c c c t c

2580 FIISNIPTQDSVDLFLAPVN a a a a t a a t a c t g t t g a t g g g t g t c t g g t c a g a g a c a t c a a g a a a t a a c g c c g g a a c a t t a t t t t a t t a t g a c a a c t a c c c a c a g a c c a g t c t c t g t a g t t c t t t a t t g c g g c c t t g t a a t

2640 TCAAEVAIADQDDLPYNIIL g t g c a g g c a g c t t c c a c a g c a a t g g c a t c c t g g t c a t c c a g c g g a t a g t t a a t g a t c a g c c a c g t c c g t c g a a g g t g t c g t t a c c g t a g g a c c a g t a g g t c g c c t a t c a a t t a c t a g t c g

2700 GSVRQALLNHVAAKCAEVGS c c a c t g a c g c g t t g c g c g a g a a g a t t g t g c a c c g c c g c t t t a c a g g c t t c g a c g c c g c t t g g t g a c t g c g c a a c g c g c t c t t c t a a c a c g t g g c g g c g a a a t g t c c g a a g c t g c g g c g a a

2760 REVMSVVVSAGLQDARSKIA c g t t c t a c c a t c g a c a c c a c c a c g c t g g c a c c c a g t t g a t c g g c g c g a g a t t t a a t c g c c g c a a g a t g g t a g c t g t g g t g g t g c g a c c g t g g g t c a a c t a g c c g c g c t c t a a a t t a g c g g

2820 AVIQSPAHLALSSTAVGILL g c g a c a a t t t g c g a c g g c g c g t g c a g g g c c a g a c t g g a g g t g g c a a c g c c a a t c a g c a a c c g c t g t t a a a c g c t g c c g c g c a c g t c c c g g t c t g a c c t c c a c c g t t g c g g t t a g t c g t t g

2880 SQKGALQQAVRNPIYNLEAM g a c t g t t t g c c c g c c a g t t g t t g t g c c a c g c g g t t g g g a a t g t a a t t c a g c t c c g c c a t c c t g a c a a a c g g g c g g t c a a c a a c a c g g t g c g c c a a c c c t t a c a t t a a g t c g a g g c g g t a g

2940 g c c g c t t c c a c t t t t t c c c g c g t t t t c g c a g a a a c g t g g c t g g c c t g g t t c a c c a c g c g g c g g c g a a g g t g a a a a a g g g c g c a a a a g c g t c t t t g c a c c g a c c g g a c c a a g t g g t g c g c c

3000 G G C A T A C T C T G C G A C A T C G T LacI-R g a a a c g g t c t g a t a a g a g a c a c c g g c a t a c t c t g c g a c a t c g t a t a a c g t t a c t g g t t t c c t t t g c c a g a c t a t t c t c t g t g g c c g t a t g a g a c g c t g t a g c a t a t t g c a a t g a c c a a a g

3060 lacI promoter a c a t t c a c c a c c c t g a a t t g a c t c t c t t c c g g g c g c t a t c a t g c c a t a c c g c g a a a g g t t t g t a a g t g g t g g g a c t t a a c t g a g a g a a g g c c c g c g a t a g t a c g g t a t g g c g c t t t c c a a

EcoO109IPaeR7IPspXIXhoIAbsISalIBspDIClaI 3120 lacI promoter KS primer C T A T G G C A G C T G G A G C T pBluescriptKS t t g c g c c a t t c g a t g g t g t c a t c a a g c t t a t c g a t a c c g t c g a c c t c g a g g g g g g g c c c g a a c g c g g t a a g c t a c c a c a g t a g t t c g a a t a g c t a t g g c a g c t g g a g c t c c c c c c c g g g c

3180 T3 promoter M13 rev C A G T A M13 Reverse g t a c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t t c g a g c t t g g c g t a a t c a t g g t c a t c a t g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a a g c t c g a a c c g c a t t a g t a c c a g t a

3240 -10 M13 rev lac operator lac promoter T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t

3300 -35 lac promoter CAP binding site g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g

3360 g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g

BspQISapI 3420 G A G C G A G T G A C T G A L4440 a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a

3480 G C G A L4440 c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g

PciI 3540 g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t

3600 ori a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c

3660 ori T T T pBR322ori-F a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t

3720 ori C T A T G G T C C G C A A A G G G pBR322ori-F g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g

3780 ori t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g

3840 ori g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g

3900 ori c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c

3960 ori t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a

4020 ori a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t

4080 ori c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a

4140 ori c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t

4200 ori t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c

4260 c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a

4320 t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a

4380 285 280 275 *WHKILSAGIEAIQR AmpR *WHKILSAGIEAIQR a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g

4440 270 265 260 255 NREDMTAQSGTTYIVVIRSP AmpR NREDMTAQSGTTYIVVIRSP t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c

BsaI 4500 250 245 240 235 KGDPGLAAIIGRSGREGAGS AmpR KGDPGLAAIIGRSGREGAGS g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c

4560 230 225 220 215 KDAIFWGAPLASRLLPGAVK AmpR KDAIFWGAPLASRLLPGAVK a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a

4620 210 205 200 195 DAEMWDILQQRSALTLLEGT AmpR DAEMWDILQQRSALTLLEGT t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c

4680 190 185 180 175 LLKRLTTAMAVPMTTDREDN AmpR LLKRLTTAMAVPMTTDREDN t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a

4740 170 165 160 155 PIAENLEPEWRDLRTVHDGM AmpR PIAENLEPEWRDLRTVHDGM t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t

4800 150 145 140 135 NHLFATLEKPGGITTLLLNA AmpR NHLFATLEKPGGITTLLLNA t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c

4860 130 125 120 115 ATNDSMTIAASCLERVTMGD AmpR ATNDSMTIAASCLERVTMGD c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a

4920 110 105 100 95 TLHKETVPSYEVLDNQSYHI AmpR TLHKETVPSYEVLDNQSYHI c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t

4980 90 85 80 75 RRGLQEQGADIRSLVAGCLL AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RRGLQEQGADIRSLVAGCLL t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t

5040 70 65 60 55 VKFTSMMPFREEPRFSELIK AmpR VKFTSMMPFREEPRFSELIK g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a

5100 50 45 40 35 GSNLDLEIYGVRAGLQDEAD AmpR GSNLDLEIYGVRAGLQDEAD t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a

5160 30 25 KVKVLTEPH 20 15 signal sequence AFVPLCFAAFF AmpR KVKVLTEPHAFVPLCFAAFF c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t

5220 10 5 1 signal sequence PILAVRFHQISM AmpR AmpR promoter PILAVRFHQISM a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a

5280 AmpR promoter g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t

5340 AmpR promoter f1 ori a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t t g t a a g c g t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t a a c a t t c g c a

5400 f1 ori t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t

5460 f1 ori g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a

5520 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c

5580 f1 ori *DLKK a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a

5640 f1 ori PDLHRLASFRFGLPLGRNLA g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g

NgoMIVNaeI 5700 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R QRPFGAFTALFSPFFAFPAP t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c

5760 f1 ori ALASALTATVSRTVVVGAAS c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a

3ʹ 5ʹ 5806 f1 ori LAGSCPADWEGNLSR t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c a g g c t g c g c a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g t c c g a c g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.